Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 224 |
| AccB1I | GGYRCC | 2 cut(s) | 224, 1054 |
| AccII | CGCG | 1 cut(s) | 294 |
| AccIII | TCCGGA | 2 cut(s) | 973, 1282 |
| AciI | CCGC | 3 cut(s) | 107, 126, 327 |
| AclWI | GGATC | 6 cut(s) | 294, 698, 896, 909, 1274, 1287 |
| AcoI | YGGCCR | 1 cut(s) | 1151 |
| AcsI | RAATTY | 5 cut(s) | 145, 260, 680, 729, 984 |
| AcuI | CTGAAG | 2 cut(s) | 602, 630 |
| AfaI | GTAC | 6 cut(s) | 226, 280, 914, 1237, 1298, 1361 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 603 |
| AflII | CTTAAG | 1 cut(s) | 319 |
| AgsI | TTSAA | 9 cut(s) | 145, 245, 463, 511, 883, 1276, 1391, 1486, 1499 |
| AluBI | AGCT | 9 cut(s) | 318, 434, 757, 857, 1061, 1468, 1490, 1517, 1521 |
| AluI | AGCT | 9 cut(s) | 318, 434, 757, 857, 1061, 1468, 1490, 1517, 1521 |
| Alw26I | GTCTC | 2 cut(s) | 912, 1325 |
| AlwI | GGATC | 6 cut(s) | 294, 698, 896, 909, 1274, 1287 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1171 |
| Ama87I | CYCGRG | 1 cut(s) | 602 |
| Aor13HI | TCCGGA | 2 cut(s) | 973, 1282 |
| AoxI | GGCC | 2 cut(s) | 68, 1151 |
| ApeKI | GCWGC | 5 cut(s) | 251, 716, 749, 857, 860 |
| ApoI | RAATTY | 5 cut(s) | 145, 260, 680, 729, 984 |
| AseI | ATTAAT | 1 cut(s) | 549 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 539, 656, 1161 |
| Asp718I | GGTACC | 1 cut(s) | 224 |
| AspA2I | CCTAGG | 1 cut(s) | 886 |
| AspLEI | GCGC | 3 cut(s) | 1074, 1255, 1375 |
| AspS9I | GGNCC | 3 cut(s) | 272, 671, 1135 |
| AsuC2I | CCSGG | 2 cut(s) | 603, 604 |
| AsuHPI | GGTGA | 3 cut(s) | 803, 958, 1210 |
| AsuNHI | GCTAGC | 1 cut(s) | 1517 |
| AvaI | CYCGRG | 1 cut(s) | 602 |
| AvaII | GGWCC | 3 cut(s) | 272, 671, 1135 |
| AvrII | CCTAGG | 1 cut(s) | 886 |
| BalI | TGGCCA | 1 cut(s) | 1153 |
| BamHI | GGATCC | 2 cut(s) | 901, 1279 |
| BanI | GGYRCC | 2 cut(s) | 224, 1054 |
| BbsI | GAAGAC | 1 cut(s) | 636 |
| BbvI | GCAGC | 5 cut(s) | 263, 728, 761, 844, 872 |
| BccI | CCATC | 6 cut(s) | 43, 166, 305, 565, 885, 1168 |
| BclI | TGATCA | 3 cut(s) | 349, 990, 1267 |
| BcnI | CCSGG | 2 cut(s) | 603, 604 |
| BcoDI | GTCTC | 2 cut(s) | 912, 1325 |
| BfaI | CTAG | 6 cut(s) | 816, 887, 1004, 1044, 1140, 1518 |
| BfmI | CTRYAG | 3 cut(s) | 493, 747, 858 |
| BfoI | RGCGCY | 1 cut(s) | 1376 |
| BfrI | CTTAAG | 1 cut(s) | 319 |
| BisI | GCNGC | 5 cut(s) | 252, 717, 750, 858, 861 |
| BlnI | CCTAGG | 1 cut(s) | 886 |
| BlsI | GCNGC | 5 cut(s) | 253, 718, 751, 859, 862 |
| BmcAI | AGTACT | 1 cut(s) | 1237 |
| Bme1390I | CCNGG | 2 cut(s) | 603, 604 |
| Bme18I | GGWCC | 3 cut(s) | 272, 671, 1135 |
| BmeT110I | CYCGRG | 1 cut(s) | 602 |
| BmgT120I | GGNCC | 3 cut(s) | 272, 671, 1135 |
| BmiI | GGNNCC | 6 cut(s) | 99, 226, 903, 1056, 1137, 1281 |
| BmrFI | CCNGG | 2 cut(s) | 603, 604 |
| BmsI | GCATC | 1 cut(s) | 1240 |
| BmtI | GCTAGC | 1 cut(s) | 1521 |
| BpiI | GAAGAC | 1 cut(s) | 636 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1062, 1464 |
| BpuMI | CCSGG | 2 cut(s) | 603, 604 |
| Bsa29I | ATCGAT | 1 cut(s) | 1434 |
| BsaJI | CCNNGG | 2 cut(s) | 602, 886 |
| BsaWI | WCCGGW | 2 cut(s) | 973, 1282 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 513, 543, 955, 985, 1335, 1365 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 603 |
| Bse3DI | GCAATG | 2 cut(s) | 711, 1510 |
| BseAI | TCCGGA | 2 cut(s) | 973, 1282 |
| BseCI | ATCGAT | 1 cut(s) | 1434 |
| BseDI | CCNNGG | 2 cut(s) | 602, 886 |
| BseGI | GGATG | 3 cut(s) | 628, 695, 896 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 603 |
| BseMI | GCAATG | 2 cut(s) | 711, 1510 |
| BseXI | GCAGC | 5 cut(s) | 263, 728, 761, 844, 872 |
| BseYI | CCCAGC | 1 cut(s) | 1368 |
| Bsh1236I | CGCG | 1 cut(s) | 294 |
| BshFI | GGCC | 2 cut(s) | 70, 1153 |
| BshNI | GGYRCC | 2 cut(s) | 224, 1054 |
| BshVI | ATCGAT | 1 cut(s) | 1434 |
| BsiHKCI | CYCGRG | 1 cut(s) | 602 |
| BsiSI | CCGG | 3 cut(s) | 603, 974, 1283 |
| BslFI | GGGAC | 1 cut(s) | 647 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 603 |
| BsmAI | GTCTC | 2 cut(s) | 912, 1325 |
| BsmBI | CGTCTC | 1 cut(s) | 1325 |
| BsmFI | GGGAC | 1 cut(s) | 647 |
| BsnI | GGCC | 2 cut(s) | 70, 1153 |
| BsoBI | CYCGRG | 1 cut(s) | 602 |
| Bsp13I | TCCGGA | 2 cut(s) | 973, 1282 |
| Bsp143I | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| BspACI | CCGC | 3 cut(s) | 107, 126, 327 |
| BspANI | GGCC | 2 cut(s) | 70, 1153 |
| BspDI | ATCGAT | 1 cut(s) | 1434 |
| BspEI | TCCGGA | 2 cut(s) | 973, 1282 |
| BspFNI | CGCG | 1 cut(s) | 294 |
| BspLI | GGNNCC | 6 cut(s) | 99, 226, 903, 1056, 1137, 1281 |
| BspMAI | CTGCAG | 2 cut(s) | 751, 862 |
| BspOI | GCTAGC | 1 cut(s) | 1521 |
| BspPI | GGATC | 6 cut(s) | 294, 698, 896, 909, 1274, 1287 |
| BspT107I | GGYRCC | 2 cut(s) | 224, 1054 |
| BspTI | CTTAAG | 1 cut(s) | 319 |
| BsrDI | GCAATG | 2 cut(s) | 711, 1510 |
| BssECI | CCNNGG | 2 cut(s) | 602, 886 |
| BssMI | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| BssT1I | CCWWGG | 1 cut(s) | 886 |
| Bst4CI | ACNGT | 3 cut(s) | 121, 362, 497 |
| Bst6I | CTCTTC | 2 cut(s) | 435, 972 |
| BstAFI | CTTAAG | 1 cut(s) | 319 |
| BstC8I | GCNNGC | 4 cut(s) | 64, 68, 1460, 1519 |
| BstDEI | CTNAG | 3 cut(s) | 1062, 1232, 1464 |
| BstF5I | GGATG | 3 cut(s) | 628, 695, 896 |
| BstFNI | CGCG | 1 cut(s) | 294 |
| BstH2I | RGCGCY | 1 cut(s) | 1376 |
| BstHHI | GCGC | 3 cut(s) | 1074, 1255, 1375 |
| BstKTI | GATC | 7 cut(s) | 289, 352, 706, 904, 993, 1270, 1282 |
| BstMAI | GTCTC | 2 cut(s) | 912, 1325 |
| BstMBI | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 59, 324, 749 |
| BstNSI | RCATGY | 1 cut(s) | 1462 |
| BstSCI | CCNGG | 2 cut(s) | 601, 602 |
| BstSFI | CTRYAG | 3 cut(s) | 493, 747, 858 |
| BstUI | CGCG | 1 cut(s) | 294 |
| BstV1I | GCAGC | 5 cut(s) | 263, 728, 761, 844, 872 |
| BstV2I | GAAGAC | 1 cut(s) | 636 |
| BstX2I | RGATCY | 3 cut(s) | 703, 901, 1279 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 282 |
| BstYI | RGATCY | 3 cut(s) | 703, 901, 1279 |
| Bsu15I | ATCGAT | 1 cut(s) | 1434 |
| BsuRI | GGCC | 2 cut(s) | 70, 1153 |
| BsuTUI | ATCGAT | 1 cut(s) | 1434 |
| BtgZI | GCGATG | 2 cut(s) | 62, 275 |
| BtsCI | GGATG | 3 cut(s) | 628, 695, 896 |
| BtsI | GCAGTG | 1 cut(s) | 744 |
| BtsIMutI | CAGTG | 2 cut(s) | 502, 744 |
| Cac8I | GCNNGC | 4 cut(s) | 64, 68, 1460, 1519 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1171 |
| CfoI | GCGC | 3 cut(s) | 1074, 1255, 1375 |
| Cfr13I | GGNCC | 3 cut(s) | 272, 671, 1135 |
| Cfr9I | CCCGGG | 1 cut(s) | 602 |
| ClaI | ATCGAT | 1 cut(s) | 1434 |
| Csp6I | GTAC | 6 cut(s) | 225, 279, 913, 1236, 1297, 1360 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 667, 702 |
| CviAII | CATG | 6 cut(s) | 282, 675, 740, 1399, 1459, 1479 |
| CviQI | GTAC | 6 cut(s) | 225, 279, 913, 1236, 1297, 1360 |
| DdeI | CTNAG | 3 cut(s) | 1062, 1232, 1464 |
| DpnI | GATC | 7 cut(s) | 288, 351, 705, 903, 992, 1269, 1281 |
| DpnII | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| DraI | TTTAAA | 1 cut(s) | 487 |
| EaeI | YGGCCR | 1 cut(s) | 1151 |
| Eam1104I | CTCTTC | 2 cut(s) | 435, 972 |
| EarI | CTCTTC | 2 cut(s) | 435, 972 |
| EciI | GGCGGA | 2 cut(s) | 141, 342 |
| Eco130I | CCWWGG | 1 cut(s) | 886 |
| Eco47I | GGWCC | 3 cut(s) | 272, 671, 1135 |
| Eco57I | CTGAAG | 2 cut(s) | 602, 630 |
| Eco88I | CYCGRG | 1 cut(s) | 602 |
| EcoT14I | CCWWGG | 1 cut(s) | 886 |
| ErhI | CCWWGG | 1 cut(s) | 886 |
| Esp3I | CGTCTC | 1 cut(s) | 1325 |
| FaeI | CATG | 6 cut(s) | 285, 678, 743, 1402, 1462, 1482 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1479, 1511 |
| FaqI | GGGAC | 1 cut(s) | 647 |
| FatI | CATG | 6 cut(s) | 281, 674, 739, 1398, 1458, 1478 |
| FbaI | TGATCA | 3 cut(s) | 349, 990, 1267 |
| Fnu4HI | GCNGC | 5 cut(s) | 252, 717, 750, 858, 861 |
| FokI | GGATG | 3 cut(s) | 635, 682, 903 |
| Fsp4HI | GCNGC | 5 cut(s) | 252, 717, 750, 858, 861 |
| FspBI | CTAG | 6 cut(s) | 816, 887, 1004, 1044, 1140, 1518 |
| GlaI | GCGC | 3 cut(s) | 1073, 1254, 1374 |
| GluI | GCNGC | 5 cut(s) | 252, 717, 750, 858, 861 |
| GsaI | CCCAGC | 1 cut(s) | 1372 |
| HaeII | RGCGCY | 1 cut(s) | 1376 |
| HaeIII | GGCC | 2 cut(s) | 70, 1153 |
| HapII | CCGG | 3 cut(s) | 603, 974, 1283 |
| HhaI | GCGC | 3 cut(s) | 1074, 1255, 1375 |
| Hin1II | CATG | 6 cut(s) | 285, 678, 743, 1402, 1462, 1482 |
| Hin6I | GCGC | 3 cut(s) | 1072, 1253, 1373 |
| HinP1I | GCGC | 3 cut(s) | 1072, 1253, 1373 |
| HindIII | AAGCTT | 4 cut(s) | 316, 755, 1466, 1488 |
| HinfI | GANTC | 5 cut(s) | 463, 801, 839, 1017, 1441 |
| HpaII | CCGG | 3 cut(s) | 603, 974, 1283 |
| HphI | GGTGA | 3 cut(s) | 803, 958, 1210 |
| Hpy166II | GTNNAC | 6 cut(s) | 500, 543, 614, 913, 1348, 1510 |
| Hpy188I | TCNGA | 5 cut(s) | 140, 193, 649, 838, 1167 |
| Hpy8I | GTNNAC | 6 cut(s) | 500, 543, 614, 913, 1348, 1510 |
| Hpy99I | CGWCG | 1 cut(s) | 1385 |
| HpyAV | CCTTC | 5 cut(s) | 83, 239, 1151, 1270, 1505 |
| HpyCH4III | ACNGT | 3 cut(s) | 121, 362, 497 |
| HpyCH4IV | ACGT | 1 cut(s) | 1350 |
| HpyCH4V | TGCA | 5 cut(s) | 415, 678, 716, 749, 860 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 59, 324, 749 |
| HpyF3I | CTNAG | 3 cut(s) | 1062, 1232, 1464 |
| HpySE526I | ACGT | 1 cut(s) | 1350 |
| Hsp92II | CATG | 6 cut(s) | 285, 678, 743, 1402, 1462, 1482 |
| HspAI | GCGC | 3 cut(s) | 1072, 1253, 1373 |
| Kpn2I | TCCGGA | 2 cut(s) | 973, 1282 |
| KpnI | GGTACC | 1 cut(s) | 228 |
| Ksp22I | TGATCA | 3 cut(s) | 349, 990, 1267 |
| Kzo9I | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| LmnI | GCTCC | 1 cut(s) | 1177 |
| Lsp1109I | GCAGC | 5 cut(s) | 263, 728, 761, 844, 872 |
| LweI | GCATC | 1 cut(s) | 1240 |
| MaeI | CTAG | 6 cut(s) | 816, 887, 1004, 1044, 1140, 1518 |
| MaeII | ACGT | 1 cut(s) | 1350 |
| MaeIII | GTNAC | 3 cut(s) | 181, 403, 455 |
| MalI | GATC | 7 cut(s) | 288, 351, 705, 903, 992, 1269, 1281 |
| MboI | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| MboII | GAAGA | 6 cut(s) | 182, 452, 636, 860, 989, 1109 |
| MfeI | CAATTG | 1 cut(s) | 1271 |
| MflI | RGATCY | 3 cut(s) | 703, 901, 1279 |
| MlsI | TGGCCA | 1 cut(s) | 1153 |
| MluNI | TGGCCA | 1 cut(s) | 1153 |
| MmeI | TCCRAC | 2 cut(s) | 125, 994 |
| MnlI | CCTC | 4 cut(s) | 70, 238, 263, 1136 |
| Mox20I | TGGCCA | 1 cut(s) | 1153 |
| MroI | TCCGGA | 2 cut(s) | 973, 1282 |
| MroXI | GAANNNNTTC | 3 cut(s) | 539, 656, 1161 |
| MscI | TGGCCA | 1 cut(s) | 1153 |
| MseI | TTAA | 6 cut(s) | 149, 320, 333, 486, 531, 549 |
| Msp20I | TGGCCA | 1 cut(s) | 1153 |
| MspCI | CTTAAG | 1 cut(s) | 319 |
| MspI | CCGG | 3 cut(s) | 603, 974, 1283 |
| MspR9I | CCNGG | 2 cut(s) | 603, 604 |
| MunI | CAATTG | 1 cut(s) | 1271 |
| MvnI | CGCG | 1 cut(s) | 294 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 59, 324, 749 |
| NciI | CCSGG | 2 cut(s) | 603, 604 |
| NdeII | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| NheI | GCTAGC | 1 cut(s) | 1517 |
| NlaIII | CATG | 6 cut(s) | 285, 678, 743, 1402, 1462, 1482 |
| NlaIV | GGNNCC | 6 cut(s) | 99, 226, 903, 1056, 1137, 1281 |
| NmuCI | GTSAC | 1 cut(s) | 403 |
| NspI | RCATGY | 1 cut(s) | 1462 |
| PaeI | GCATGC | 1 cut(s) | 1462 |
| PdmI | GAANNNNTTC | 3 cut(s) | 539, 656, 1161 |
| PfeI | GAWTC | 5 cut(s) | 463, 801, 839, 1017, 1441 |
| PkrI | GCNGC | 5 cut(s) | 253, 718, 751, 859, 862 |
| PshBI | ATTAAT | 1 cut(s) | 549 |
| PspFI | CCCAGC | 1 cut(s) | 1368 |
| PspN4I | GGNNCC | 6 cut(s) | 99, 226, 903, 1056, 1137, 1281 |
| PspPI | GGNCC | 3 cut(s) | 272, 671, 1135 |
| PstI | CTGCAG | 2 cut(s) | 751, 862 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1171 |
| PsuI | RGATCY | 3 cut(s) | 703, 901, 1279 |
| RsaI | GTAC | 6 cut(s) | 226, 280, 914, 1237, 1298, 1361 |
| RsaNI | GTAC | 6 cut(s) | 225, 279, 913, 1236, 1297, 1360 |
| SaqAI | TTAA | 6 cut(s) | 149, 320, 333, 486, 531, 549 |
| SatI | GCNGC | 5 cut(s) | 252, 717, 750, 858, 861 |
| Sau3AI | GATC | 7 cut(s) | 286, 349, 703, 901, 990, 1267, 1279 |
| Sau96I | GGNCC | 3 cut(s) | 272, 671, 1135 |
| ScaI | AGTACT | 1 cut(s) | 1237 |
| ScrFI | CCNGG | 2 cut(s) | 603, 604 |
| SfaNI | GCATC | 1 cut(s) | 1240 |
| SfcI | CTRYAG | 3 cut(s) | 493, 747, 858 |
| SinI | GGWCC | 3 cut(s) | 272, 671, 1135 |
| SmaI | CCCGGG | 1 cut(s) | 604 |
| SmlI | CTYRAG | 1 cut(s) | 319 |
| SmoI | CTYRAG | 1 cut(s) | 319 |
| SphI | GCATGC | 1 cut(s) | 1462 |
| SsiI | CCGC | 3 cut(s) | 107, 126, 327 |
| SspI | AATATT | 1 cut(s) | 1122 |
| SspMI | CTAG | 6 cut(s) | 816, 887, 1004, 1044, 1140, 1518 |
| StyD4I | CCNGG | 2 cut(s) | 601, 602 |
| StyI | CCWWGG | 1 cut(s) | 886 |
| TaaI | ACNGT | 3 cut(s) | 121, 362, 497 |
| TaiI | ACGT | 1 cut(s) | 1353 |
| TaqI | TCGA | 5 cut(s) | 75, 168, 876, 1380, 1434 |
| TatI | WGTACW | 2 cut(s) | 278, 1235 |
| TfiI | GAWTC | 5 cut(s) | 463, 801, 839, 1017, 1441 |
| Tru1I | TTAA | 6 cut(s) | 149, 320, 333, 486, 531, 549 |
| Tru9I | TTAA | 6 cut(s) | 149, 320, 333, 486, 531, 549 |
| TscAI | CASTG | 2 cut(s) | 502, 751 |
| TseFI | GTSAC | 1 cut(s) | 403 |
| TseI | GCWGC | 5 cut(s) | 251, 716, 749, 857, 860 |
| Tsp45I | GTSAC | 1 cut(s) | 403 |
| TspDTI | ATGAA | 3 cut(s) | 597, 684, 950 |
| TspGWI | ACGGA | 1 cut(s) | 930 |
| TspMI | CCCGGG | 1 cut(s) | 602 |
| TspRI | CASTG | 2 cut(s) | 502, 751 |
| Vha464I | CTTAAG | 1 cut(s) | 319 |
| VpaK11BI | GGWCC | 3 cut(s) | 272, 671, 1135 |
| VspI | ATTAAT | 1 cut(s) | 549 |
| XapI | RAATTY | 5 cut(s) | 145, 260, 680, 729, 984 |
| XbaI | TCTAGA | 2 cut(s) | 815, 1003 |
| XceI | RCATGY | 1 cut(s) | 1462 |
| XmaI | CCCGGG | 1 cut(s) | 602 |
| XmaJI | CCTAGG | 1 cut(s) | 886 |
| XmnI | GAANNNNTTC | 3 cut(s) | 539, 656, 1161 |
| XspI | CTAG | 6 cut(s) | 816, 887, 1004, 1044, 1140, 1518 |
| ZrmI | AGTACT | 1 cut(s) | 1237 |