Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 960, 1434 |
| AclWI | GGATC | 6 cut(s) | 217, 1041, 1054, 1274, 1287, 1328 |
| AcsI | RAATTY | 2 cut(s) | 276, 1290 |
| AcuI | CTGAAG | 3 cut(s) | 150, 365, 1146 |
| AfaI | GTAC | 6 cut(s) | 187, 711, 730, 944, 1111, 1316 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 390, 1418 |
| AgsI | TTSAA | 5 cut(s) | 206, 580, 824, 932, 1295 |
| AjnI | CCWGG | 4 cut(s) | 383, 389, 429, 698 |
| AluBI | AGCT | 7 cut(s) | 77, 456, 527, 805, 905, 1115, 1124 |
| AluI | AGCT | 7 cut(s) | 77, 456, 527, 805, 905, 1115, 1124 |
| Alw21I | GWGCWC | 1 cut(s) | 718 |
| AlwI | GGATC | 6 cut(s) | 217, 1041, 1054, 1274, 1287, 1328 |
| ApeKI | GCWGC | 4 cut(s) | 191, 194, 905, 1121 |
| ApoI | RAATTY | 2 cut(s) | 276, 1290 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 225, 257 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 276 |
| AspS9I | GGNCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| AsuHPI | GGTGA | 2 cut(s) | 6, 1009 |
| AvaII | GGWCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| BamHI | GGATCC | 2 cut(s) | 1046, 1279 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1178, 1210 |
| BbsI | GAAGAC | 1 cut(s) | 903 |
| Bbv12I | GWGCWC | 1 cut(s) | 718 |
| BbvI | GCAGC | 4 cut(s) | 178, 181, 892, 1133 |
| BccI | CCATC | 3 cut(s) | 311, 933, 1321 |
| BceAI | ACGGC | 2 cut(s) | 301, 496 |
| BciT130I | CCWGG | 4 cut(s) | 385, 391, 431, 700 |
| BfmI | CTRYAG | 3 cut(s) | 792, 906, 1163 |
| BisI | GCNGC | 4 cut(s) | 192, 195, 906, 1122 |
| BlsI | GCNGC | 4 cut(s) | 193, 196, 907, 1123 |
| Bme1390I | CCNGG | 4 cut(s) | 385, 391, 431, 700 |
| Bme18I | GGWCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| BmgT120I | GGNCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| BmiI | GGNNCC | 5 cut(s) | 248, 951, 1048, 1281, 1484 |
| BmrFI | CCNGG | 4 cut(s) | 385, 391, 431, 700 |
| BmsI | GCATC | 5 cut(s) | 14, 71, 128, 492, 1074 |
| BpiI | GAAGAC | 1 cut(s) | 903 |
| BpuEI | CTTGAG | 3 cut(s) | 145, 458, 1025 |
| Bsa29I | ATCGAT | 1 cut(s) | 306 |
| BsaBI | GATNNNNATC | 1 cut(s) | 638 |
| BsaJI | CCNNGG | 2 cut(s) | 352, 1236 |
| BsaWI | WCCGGW | 1 cut(s) | 1317 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 606, 636 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 390, 1418 |
| Bse3DI | GCAATG | 1 cut(s) | 48 |
| Bse8I | GATNNNNATC | 1 cut(s) | 638 |
| BseBI | CCWGG | 4 cut(s) | 385, 391, 431, 700 |
| BseCI | ATCGAT | 1 cut(s) | 306 |
| BseDI | CCNNGG | 2 cut(s) | 352, 1236 |
| BseGI | GGATG | 5 cut(s) | 5, 299, 577, 944, 1150 |
| BseJI | GATNNNNATC | 1 cut(s) | 638 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 390, 1418 |
| BseMI | GCAATG | 1 cut(s) | 48 |
| BseXI | GCAGC | 4 cut(s) | 178, 181, 892, 1133 |
| BsgI | GTGCAG | 1 cut(s) | 180 |
| BshVI | ATCGAT | 1 cut(s) | 306 |
| BsiHKAI | GWGCWC | 1 cut(s) | 718 |
| BsiSI | CCGG | 1 cut(s) | 1318 |
| BslFI | GGGAC | 1 cut(s) | 260 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 390, 1418 |
| BsmFI | GGGAC | 1 cut(s) | 260 |
| BsmI | GAATGC | 2 cut(s) | 91, 1458 |
| Bsp1286I | GDGCHC | 1 cut(s) | 718 |
| Bsp143I | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| BspDI | ATCGAT | 1 cut(s) | 306 |
| BspHI | TCATGA | 1 cut(s) | 1182 |
| BspLI | GGNNCC | 5 cut(s) | 248, 951, 1048, 1281, 1484 |
| BspMAI | CTGCAG | 2 cut(s) | 910, 1167 |
| BspPI | GGATC | 6 cut(s) | 217, 1041, 1054, 1274, 1287, 1328 |
| BsrDI | GCAATG | 1 cut(s) | 48 |
| BssECI | CCNNGG | 2 cut(s) | 352, 1236 |
| BssMI | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| BssNAI | GTATAC | 1 cut(s) | 961 |
| BssT1I | CCWWGG | 1 cut(s) | 352 |
| Bst1107I | GTATAC | 1 cut(s) | 961 |
| Bst2UI | CCWGG | 4 cut(s) | 385, 391, 431, 700 |
| Bst4CI | ACNGT | 3 cut(s) | 590, 733, 796 |
| Bst6I | CTCTTC | 1 cut(s) | 1020 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 109 |
| BstDEI | CTNAG | 3 cut(s) | 663, 1488, 1509 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 388 |
| BstF5I | GGATG | 5 cut(s) | 5, 299, 577, 944, 1150 |
| BstKTI | GATC | 4 cut(s) | 212, 1049, 1282, 1323 |
| BstMBI | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 59, 109, 221, 695, 1112, 1121 |
| BstNI | CCWGG | 4 cut(s) | 385, 391, 431, 700 |
| BstSCI | CCNGG | 4 cut(s) | 383, 389, 429, 698 |
| BstSFI | CTRYAG | 3 cut(s) | 792, 906, 1163 |
| BstV1I | GCAGC | 4 cut(s) | 178, 181, 892, 1133 |
| BstV2I | GAAGAC | 1 cut(s) | 903 |
| BstX2I | RGATCY | 2 cut(s) | 1046, 1279 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 243 |
| BstYI | RGATCY | 2 cut(s) | 1046, 1279 |
| BstZ17I | GTATAC | 1 cut(s) | 961 |
| Bsu15I | ATCGAT | 1 cut(s) | 306 |
| BsuTUI | ATCGAT | 1 cut(s) | 306 |
| BtsCI | GGATG | 5 cut(s) | 5, 299, 577, 944, 1150 |
| BtsIMutI | CAGTG | 1 cut(s) | 595 |
| CciI | TCATGA | 1 cut(s) | 1182 |
| Cfr13I | GGNCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| ClaI | ATCGAT | 1 cut(s) | 306 |
| Csp6I | GTAC | 6 cut(s) | 186, 710, 729, 943, 1110, 1315 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 510, 545 |
| CviAII | CATG | 4 cut(s) | 726, 788, 946, 1183 |
| CviQI | GTAC | 6 cut(s) | 186, 710, 729, 943, 1110, 1315 |
| DdeI | CTNAG | 3 cut(s) | 663, 1488, 1509 |
| DpnI | GATC | 4 cut(s) | 211, 1048, 1281, 1322 |
| DpnII | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| DraI | TTTAAA | 1 cut(s) | 604 |
| Eam1104I | CTCTTC | 1 cut(s) | 1020 |
| EarI | CTCTTC | 1 cut(s) | 1020 |
| Eco130I | CCWWGG | 1 cut(s) | 352 |
| Eco47I | GGWCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| Eco57I | CTGAAG | 3 cut(s) | 150, 365, 1146 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 388 |
| EcoO109I | RGGNCCY | 1 cut(s) | 349 |
| EcoRI | GAATTC | 2 cut(s) | 276, 1290 |
| EcoRII | CCWGG | 4 cut(s) | 383, 389, 429, 698 |
| EcoT14I | CCWWGG | 1 cut(s) | 352 |
| EcoT22I | ATGCAT | 1 cut(s) | 121 |
| ErhI | CCWWGG | 1 cut(s) | 352 |
| FaeI | CATG | 4 cut(s) | 729, 791, 949, 1186 |
| FaqI | GGGAC | 1 cut(s) | 260 |
| FatI | CATG | 4 cut(s) | 725, 787, 945, 1182 |
| FblI | GTMKAC | 2 cut(s) | 960, 1434 |
| Fnu4HI | GCNGC | 4 cut(s) | 192, 195, 906, 1122 |
| FokI | GGATG | 4 cut(s) | 286, 584, 951, 1137 |
| Fsp4HI | GCNGC | 4 cut(s) | 192, 195, 906, 1122 |
| GluI | GCNGC | 4 cut(s) | 192, 195, 906, 1122 |
| HapII | CCGG | 1 cut(s) | 1318 |
| Hin1II | CATG | 4 cut(s) | 729, 791, 949, 1186 |
| HincII | GTYRAC | 1 cut(s) | 1435 |
| HindII | GTYRAC | 1 cut(s) | 1435 |
| HindIII | AAGCTT | 1 cut(s) | 525 |
| HinfI | GANTC | 8 cut(s) | 180, 308, 362, 394, 849, 887, 1416, 1469 |
| HpaII | CCGG | 1 cut(s) | 1318 |
| HphI | GGTGA | 2 cut(s) | 6, 1009 |
| Hpy166II | GTNNAC | 6 cut(s) | 427, 593, 839, 961, 1259, 1435 |
| Hpy188I | TCNGA | 5 cut(s) | 130, 154, 345, 886, 1054 |
| Hpy188III | TCNNGA | 6 cut(s) | 281, 475, 625, 1154, 1183, 1324 |
| Hpy8I | GTNNAC | 6 cut(s) | 427, 593, 839, 961, 1259, 1435 |
| Hpy99I | CGWCG | 1 cut(s) | 1436 |
| HpyAV | CCTTC | 9 cut(s) | 200, 331, 340, 428, 785, 1121, 1151, 1180, 1394 |
| HpyCH4III | ACNGT | 3 cut(s) | 590, 733, 796 |
| HpyCH4IV | ACGT | 3 cut(s) | 718, 1255, 1431 |
| HpyCH4V | TGCA | 7 cut(s) | 62, 103, 112, 119, 197, 908, 1165 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 59, 109, 221, 695, 1112, 1121 |
| HpyF3I | CTNAG | 3 cut(s) | 663, 1488, 1509 |
| HpySE526I | ACGT | 3 cut(s) | 718, 1255, 1431 |
| Hsp92II | CATG | 4 cut(s) | 729, 791, 949, 1186 |
| Kzo9I | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| LmnI | GCTCC | 4 cut(s) | 82, 694, 949, 1488 |
| Lsp1109I | GCAGC | 4 cut(s) | 178, 181, 892, 1133 |
| LweI | GCATC | 5 cut(s) | 14, 71, 128, 492, 1074 |
| MaeII | ACGT | 3 cut(s) | 718, 1255, 1431 |
| MaeIII | GTNAC | 2 cut(s) | 69, 496 |
| MalI | GATC | 4 cut(s) | 211, 1048, 1281, 1322 |
| MboI | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| MboII | GAAGA | 6 cut(s) | 143, 341, 389, 908, 1037, 1042 |
| MflI | RGATCY | 2 cut(s) | 1046, 1279 |
| MhlI | GDGCHC | 1 cut(s) | 718 |
| MlyI | GAGTC | 2 cut(s) | 189, 1463 |
| MmeI | TCCRAC | 1 cut(s) | 841 |
| MnlI | CCTC | 8 cut(s) | 19, 87, 141, 528, 539, 1060, 1231, 1498 |
| Mph1103I | ATGCAT | 1 cut(s) | 121 |
| MroXI | GAANNNNTTC | 1 cut(s) | 276 |
| MseI | TTAA | 2 cut(s) | 603, 1092 |
| MspA1I | CMGCKG | 1 cut(s) | 1124 |
| MspI | CCGG | 1 cut(s) | 1318 |
| MspR9I | CCNGG | 4 cut(s) | 385, 391, 431, 700 |
| Mva1269I | GAATGC | 2 cut(s) | 91, 1458 |
| MvaI | CCWGG | 4 cut(s) | 385, 391, 431, 700 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 59, 109, 221, 695, 1112, 1121 |
| NdeII | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| NlaIII | CATG | 4 cut(s) | 729, 791, 949, 1186 |
| NlaIV | GGNNCC | 5 cut(s) | 248, 951, 1048, 1281, 1484 |
| NmeAIII | GCCGAG | 1 cut(s) | 115 |
| NmuCI | GTSAC | 2 cut(s) | 69, 496 |
| NsiI | ATGCAT | 1 cut(s) | 121 |
| PagI | TCATGA | 1 cut(s) | 1182 |
| PctI | GAATGC | 2 cut(s) | 91, 1458 |
| PdmI | GAANNNNTTC | 1 cut(s) | 276 |
| PfeI | GAWTC | 6 cut(s) | 308, 362, 394, 849, 887, 1416 |
| PfoI | TCCNGGA | 1 cut(s) | 383 |
| PkrI | GCNGC | 4 cut(s) | 193, 196, 907, 1123 |
| PleI | GAGTC | 2 cut(s) | 188, 1463 |
| PpsI | GAGTC | 2 cut(s) | 188, 1463 |
| PpuMI | RGGWCCY | 1 cut(s) | 349 |
| Psp5II | RGGWCCY | 1 cut(s) | 349 |
| Psp6I | CCWGG | 4 cut(s) | 383, 389, 429, 698 |
| PspGI | CCWGG | 4 cut(s) | 383, 389, 429, 698 |
| PspN4I | GGNNCC | 5 cut(s) | 248, 951, 1048, 1281, 1484 |
| PspPI | GGNCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| PspPPI | RGGWCCY | 1 cut(s) | 349 |
| PstI | CTGCAG | 2 cut(s) | 910, 1167 |
| PsuI | RGATCY | 2 cut(s) | 1046, 1279 |
| PvuII | CAGCTG | 1 cut(s) | 1124 |
| RsaI | GTAC | 6 cut(s) | 187, 711, 730, 944, 1111, 1316 |
| RsaNI | GTAC | 6 cut(s) | 186, 710, 729, 943, 1110, 1315 |
| SalI | GTCGAC | 1 cut(s) | 1433 |
| SaqAI | TTAA | 2 cut(s) | 603, 1092 |
| SatI | GCNGC | 4 cut(s) | 192, 195, 906, 1122 |
| Sau3AI | GATC | 4 cut(s) | 209, 1046, 1279, 1320 |
| Sau96I | GGNCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| SchI | GAGTC | 2 cut(s) | 189, 1463 |
| ScrFI | CCNGG | 4 cut(s) | 385, 391, 431, 700 |
| SduI | GDGCHC | 1 cut(s) | 718 |
| SfaNI | GCATC | 5 cut(s) | 14, 71, 128, 492, 1074 |
| SfcI | CTRYAG | 3 cut(s) | 792, 906, 1163 |
| SinI | GGWCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| SmlI | CTYRAG | 3 cut(s) | 160, 473, 1040 |
| SmoI | CTYRAG | 3 cut(s) | 160, 473, 1040 |
| StyD4I | CCNGG | 4 cut(s) | 383, 389, 429, 698 |
| StyI | CCWWGG | 1 cut(s) | 352 |
| TaaI | ACNGT | 3 cut(s) | 590, 733, 796 |
| TaiI | ACGT | 3 cut(s) | 721, 1258, 1434 |
| TaqI | TCGA | 3 cut(s) | 306, 513, 1434 |
| TatI | WGTACW | 2 cut(s) | 185, 1109 |
| TfiI | GAWTC | 6 cut(s) | 308, 362, 394, 849, 887, 1416 |
| Tru1I | TTAA | 2 cut(s) | 603, 1092 |
| Tru9I | TTAA | 2 cut(s) | 603, 1092 |
| TscAI | CASTG | 1 cut(s) | 595 |
| TseFI | GTSAC | 2 cut(s) | 69, 496 |
| TseI | GCWGC | 4 cut(s) | 191, 194, 905, 1121 |
| Tsp45I | GTSAC | 2 cut(s) | 69, 496 |
| TspDTI | ATGAA | 4 cut(s) | 300, 480, 1171, 1199 |
| TspGWI | ACGGA | 1 cut(s) | 978 |
| TspRI | CASTG | 1 cut(s) | 595 |
| VpaK11BI | GGWCC | 5 cut(s) | 233, 247, 349, 387, 863 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 388 |
| XapI | RAATTY | 2 cut(s) | 276, 1290 |
| XmiI | GTMKAC | 2 cut(s) | 960, 1434 |
| XmnI | GAANNNNTTC | 1 cut(s) | 276 |
| Zsp2I | ATGCAT | 1 cut(s) | 121 |