MD17G1089500.v1.1

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000349
Physical Location & Seq
Reverse (-)
7350280 .. 7353141
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1089500.v1.1.491

Sequence Viewer

Length: 972 bp
ATGTACGATTTCCCATTCTCTGTTCTGAGAAATGGGTGTGTTGAGATATTGAGCCTCACCAGGTGTTATCTCAATCTGCCGGCGAATTTGTCCACCATGCGGTTCCGGTCGATTGCGTCAATGGTTCTCGATGACGTTTATTTGACGGACCAGATGGTGGAGGATTTGCTTCTGGGTTGCCCCAATTTGGAGGCTTTCGAGATTCTTGGGTGTCGCGGGCCAATTCATTTGAAGATATGCAGCAAAGGGCTCAAAAGGCTGCGAATTGTATTGGGGTATTATTTTCCTGATGATCCTGAAAAGAGACAGACATTTTTGGTTGATTGCCCCAACATTTGTTCGATCAATTTGGATCTTTGTGCCTTTGAGGAGTCTACTTTTAAGAATGTTTCTTCCCTGGTTGAGTTTCGTGCCGATTTCGTGCACCGAATCCATCAGTCTTATCATCAGTGGAGTAAGGTTATTAGGGTTCTCGAACAAGCGCCTAATCTTAAGATTCTCAATGTGCAAAATTGGTGGTTTAAGTTTCTGACATCAAAGGATACATTCCCCAAAAGTTTCATGCTCCACAACCTGACGGTCTTAGTGCTGCGAACGCGGTTTACCCAATATGATCTCGTTGGCATGGCTGCAATGCTTAAACTTTGTCCCAATCTCAAGACACTGATTCTCGAATTCCTTTTCAAGGTGGAGAAAGATGAGAGTTTACCAGAAGAGTTGTTAGATAAACCGGTCGAGTTCAACATGCCATGTCTCAAGCAAGTAACAATGGAAGCTTATACTGGGAGCGAAGACGATGATAAGTTTATGAAAATCTTGATGAGGCATGAAGGCGCCCTAGAAAAGATTGTAGTTGTTCCCTTCCAAGGTTTCGAGAATGGCGAGAAGAAGTCGCTTCCTCCGGTGGTATATAGAAGAAGGGTCTCGCAAGTTGGAAGTACGGTCTCCTCCGCAGCACCAAATGGTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

324

Amino Acids

37.17

Weight (kDa)

6.99

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 2 - 259 3.1e-15 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 5 - 222 2.7e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43415
fragaria_vesca FvH4_1g28610 FvH4_6g43940 FvH4_6g43940 FvH4_6g43940 FvH4_6g43950 FvH4_6g43960 FvH4_6g43970 FvH4_6g43990 FvH4_6g44000 FvH4_7g14510
malus_domestica MD09G1101800.v1.1 MD17G1089200.v1.1 MD17G1089300.v1.1 MD17G1089400.v1.1 MD17G1089500.v1.1 MD17G1089600.v1.1
prunus_persica Prupe.3G223900_v2.0.a1 Prupe.3G223900_v2.0.a1 Prupe.3G224000_v2.0.a1 Prupe.3G224100_v2.0.a1 Prupe.3G224200_v2.0.a1
pyrus_communis pycom09g02550 pycom17g02610 pycom17g08610 pycom17g08620 pycom17g08630 pycom17g08640 pycom17g08670
rosa_chinensis RchiOBHm_Chr2g0160711 RchiOBHm_Chr2g0160721 RchiOBHm_Chr2g0160731 RchiOBHm_Chr2g0160741 RchiOBHm_Chr4g0433831 RchiOBHm_Chr7g0197991
rosa_laevigata RLG00000001614 RLG00000002340 RLG00000003895 RLG00000005223 RLG00000021235 RLG00000021236 RLG00000021237 RLG00000021238 RLG00000028664 RLG00000029542 RLG00000029654 RLG00000035034
rosa_multiflora Rmu_co8177938.1_g000001 Rmu_co8474907.1_g000002 Rmu_sc0000064.1_g000011 Rmu_sc0000221.1_g000038 Rmu_sc0002773.1_g000001 Rmu_sc0002773.1_g000004 Rmu_sc0002773.1_g000006 Rmu_sc0002773.1_g000008 Rmu_sc0003465.1_g000005 Rmu_sc0006325.1_g000010 Rmu_sc0024525.1_g000002 Rmu_sc0030848.1_g000001 Rmu_sc0030848.1_g000002 Rmu_sc0030848.1_g000004
rosa_roxburghii Rroxscaffold_2G00089410 Rroxscaffold_2G00089420 Rroxscaffold_2G00089430 Rroxscaffold_2G00089450 Rroxscaffold_2G00089460 Rroxscaffold_3G00258030 Rroxscaffold_5G00351500
rosa_rugosa Rorug02G0485200 Rorug02G0485400 Rorug02G0485500 Rorug02G0485600 Rorug06G0149200
rosa_samantha Rh1AG011000 Rh2AG501800 Rh2AG550200 Rh2AG550300 Rh2AG550400 Rh2AG550500 Rh2BG217000 Rh2BG564800 Rh2BG564900 Rh2BG565000 Rh2BG565100 Rh2BG607100 Rh2CG487700 Rh2CG534600 Rh2CG534700 Rh2CG534800 Rh2CG534900 Rh2DG573500 Rh2DG573600 Rh2DG574000 Rh2DG574100 Rh3CG290600 Rh7AG168600 Rh7BG171600 Rh7BG171700 Rh7CG178100 Rh7DG170700 Rh7DG170900
rosa_wichuraiana Rw2G022070 Rw2G045590 Rw2G045600 Rw2G045610 Rw2G045620 Rw3G021600 Rw7G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 157
AccI GTMKAC 1 cut(s) 374
AccII CGCG 2 cut(s) 216, 598
AciI CCGC 4 cut(s) 100, 216, 598, 951
AclWI GGATC 2 cut(s) 287, 360
AcsI RAATTY 2 cut(s) 85, 674
AcyI GRCGYC 1 cut(s) 834
AdeI CACNNNGTG 1 cut(s) 63
AfaI GTAC 2 cut(s) 5, 940
AfiI CCNNNNNNNGG 5 cut(s) 99, 157, 187, 685, 866
AflII CTTAAG 1 cut(s) 491
AgeI ACCGGT 1 cut(s) 730
AgsI TTSAA 3 cut(s) 232, 685, 742
AjnI CCWGG 2 cut(s) 59, 396
AluBI AGCT 1 cut(s) 776
AluI AGCT 1 cut(s) 776
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 4 cut(s) 298, 758, 928, 949
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 2 cut(s) 287, 360
AoxI GGCC 1 cut(s) 218
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 5 cut(s) 240, 259, 589, 629, 953
ApoI RAATTY 2 cut(s) 85, 674
AsiGI ACCGGT 1 cut(s) 730
AspLEI GCGC 2 cut(s) 484, 836
AspS9I GGNCC 2 cut(s) 148, 218
AsuHPI GGTGA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 426
BanI GGYRCC 1 cut(s) 833
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 5 cut(s) 246, 252, 576, 616, 965
BccI CCATC 2 cut(s) 148, 441
BciT130I CCWGG 2 cut(s) 61, 398
BciVI GTATCC 1 cut(s) 535
BcoDI GTCTC 4 cut(s) 298, 758, 928, 949
BfaI CTAG 1 cut(s) 839
BfoI RGCGCY 2 cut(s) 485, 837
BfrI CTTAAG 1 cut(s) 491
BfuI GTATCC 1 cut(s) 535
BisI GCNGC 5 cut(s) 241, 260, 590, 630, 954
BlsI GCNGC 5 cut(s) 242, 261, 591, 631, 955
Bme1390I CCNGG 2 cut(s) 61, 398
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 148, 218
BmiI GGNNCC 2 cut(s) 104, 835
BmrFI CCNGG 2 cut(s) 61, 398
BmrI ACTGGG 1 cut(s) 792
BmuI ACTGGG 1 cut(s) 792
BpiI GAAGAC 1 cut(s) 798
BpuEI CTTGAG 2 cut(s) 641, 740
BsaHI GRCGYC 1 cut(s) 834
BsaI GGTCTC 2 cut(s) 928, 949
BsaJI CCNNGG 2 cut(s) 396, 865
BsaWI WCCGGW 3 cut(s) 105, 730, 901
Bsc4I CCNNNNNNNGG 5 cut(s) 99, 157, 187, 685, 866
Bse118I RCCGGY 2 cut(s) 79, 730
Bse1I ACTGG 1 cut(s) 787
Bse3DI GCAATG 1 cut(s) 639
BseBI CCWGG 2 cut(s) 61, 398
BseDI CCNNGG 2 cut(s) 396, 865
BseLI CCNNNNNNNGG 5 cut(s) 99, 157, 187, 685, 866
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 1 cut(s) 17
BseNI ACTGG 1 cut(s) 787
BseRI GAGGAG 2 cut(s) 383, 937
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 5 cut(s) 246, 252, 576, 616, 965
Bsh1236I CGCG 2 cut(s) 216, 598
Bsh1285I CGRYCG 2 cut(s) 110, 735
BshFI GGCC 1 cut(s) 220
BshNI GGYRCC 1 cut(s) 833
BshTI ACCGGT 1 cut(s) 730
BsiEI CGRYCG 2 cut(s) 110, 735
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 4 cut(s) 80, 106, 731, 902
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 5 cut(s) 99, 157, 187, 685, 866
BsmAI GTCTC 4 cut(s) 298, 758, 928, 949
BsmFI GGGAC 1 cut(s) 633
BsnI GGCC 1 cut(s) 220
Bso31I GGTCTC 2 cut(s) 928, 949
Bsp1286I GDGCHC 2 cut(s) 252, 426
Bsp143I GATC 4 cut(s) 292, 342, 352, 613
BspACI CCGC 4 cut(s) 100, 216, 598, 951
BspANI GGCC 1 cut(s) 220
BspCNI CTCAG 1 cut(s) 18
BspFNI CGCG 2 cut(s) 216, 598
BspLI GGNNCC 2 cut(s) 104, 835
BspPI GGATC 2 cut(s) 287, 360
BspT107I GGYRCC 1 cut(s) 833
BspTI CTTAAG 1 cut(s) 491
BspTNI GGTCTC 2 cut(s) 928, 949
BsrDI GCAATG 1 cut(s) 639
BsrFI RCCGGY 2 cut(s) 79, 730
BsrI ACTGG 1 cut(s) 787
BssAI RCCGGY 2 cut(s) 79, 730
BssECI CCNNGG 2 cut(s) 396, 865
BssMI GATC 4 cut(s) 292, 342, 352, 613
BssNI GRCGYC 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 865
Bst2UI CCWGG 2 cut(s) 61, 398
Bst4CI ACNGT 2 cut(s) 580, 943
Bst6I CTCTTC 1 cut(s) 708
BstACI GRCGYC 1 cut(s) 834
BstAFI CTTAAG 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 81, 218
BstDEI CTNAG 2 cut(s) 26, 583
BstENI CCTNNNNNAGG 1 cut(s) 683
BstFNI CGCG 2 cut(s) 216, 598
BstH2I RGCGCY 2 cut(s) 485, 837
BstHHI GCGC 2 cut(s) 484, 836
BstKTI GATC 4 cut(s) 295, 345, 355, 616
BstMAI GTCTC 4 cut(s) 298, 758, 928, 949
BstMBI GATC 4 cut(s) 292, 342, 352, 613
BstMCI CGRYCG 2 cut(s) 110, 735
BstMWI GCNNNNNNNGC 2 cut(s) 256, 595
BstNI CCWGG 2 cut(s) 61, 398
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 2 cut(s) 59, 396
BstSLI GKGCMC 1 cut(s) 426
BstUI CGCG 2 cut(s) 216, 598
BstV1I GCAGC 5 cut(s) 246, 252, 576, 616, 965
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 220
BtsIMutI CAGTG 2 cut(s) 455, 662
Cac8I GCNNGC 2 cut(s) 81, 218
CfoI GCGC 2 cut(s) 484, 836
Cfr10I RCCGGY 2 cut(s) 79, 730
Cfr13I GGNCC 2 cut(s) 148, 218
CseI GACGC 1 cut(s) 105
CsiI ACCWGGT 1 cut(s) 59
Csp6I GTAC 2 cut(s) 4, 939
CspAI ACCGGT 1 cut(s) 730
CspCI CAANNNNNGTGG 2 cut(s) 497, 532
CviAII CATG 6 cut(s) 97, 562, 625, 745, 750, 827
CviJI RGCY 7 cut(s) 54, 194, 220, 250, 259, 629, 776
CviKI_1 RGCY 7 cut(s) 54, 194, 220, 250, 259, 629, 776
CviQI GTAC 2 cut(s) 4, 939
DdeI CTNAG 2 cut(s) 26, 583
DinI GGCGCC 1 cut(s) 835
DpnI GATC 4 cut(s) 294, 344, 354, 615
DpnII GATC 4 cut(s) 292, 342, 352, 613
DraIII CACNNNGTG 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 865
Eco24I GRGCYC 1 cut(s) 252
Eco31I GGTCTC 2 cut(s) 928, 949
Eco47I GGWCC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 59, 396
EcoT14I CCWWGG 1 cut(s) 865
EcoT38I GRGCYC 1 cut(s) 252
EgeI GGCGCC 1 cut(s) 835
EheI GGCGCC 1 cut(s) 835
ErhI CCWWGG 1 cut(s) 865
FaeI CATG 6 cut(s) 100, 565, 628, 748, 753, 830
FaqI GGGAC 1 cut(s) 633
FatI CATG 6 cut(s) 96, 561, 624, 744, 749, 826
FauI CCCGC 1 cut(s) 209
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 5 cut(s) 241, 260, 590, 630, 954
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 5 cut(s) 241, 260, 590, 630, 954
FspBI CTAG 1 cut(s) 839
GlaI GCGC 2 cut(s) 483, 835
GluI GCNGC 5 cut(s) 241, 260, 590, 630, 954
HaeII RGCGCY 2 cut(s) 485, 837
HaeIII GGCC 1 cut(s) 220
HapII CCGG 4 cut(s) 80, 106, 731, 902
HgaI GACGC 1 cut(s) 105
HhaI GCGC 2 cut(s) 484, 836
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 6 cut(s) 100, 565, 628, 748, 753, 830
Hin6I GCGC 2 cut(s) 482, 834
HinP1I GCGC 2 cut(s) 482, 834
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 5 cut(s) 202, 371, 429, 496, 667
HpaII CCGG 4 cut(s) 80, 106, 731, 902
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 5 cut(s) 93, 375, 424, 603, 707
Hpy188I TCNGA 2 cut(s) 27, 531
Hpy188III TCNNGA 9 cut(s) 128, 199, 287, 296, 473, 658, 671, 817, 874
Hpy8I GTNNAC 5 cut(s) 93, 375, 424, 603, 707
HpyAV CCTTC 3 cut(s) 824, 871, 912
HpyCH4III ACNGT 2 cut(s) 580, 943
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 240, 424, 508, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 256, 595
HpyF3I CTNAG 2 cut(s) 26, 583
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 6 cut(s) 100, 565, 628, 748, 753, 830
HspAI GCGC 2 cut(s) 482, 834
KasI GGCGCC 1 cut(s) 833
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Kzo9I GATC 4 cut(s) 292, 342, 352, 613
LmnI GCTCC 2 cut(s) 570, 786
Lsp1109I GCAGC 5 cut(s) 246, 252, 576, 616, 965
MabI ACCWGGT 1 cut(s) 59
MaeI CTAG 1 cut(s) 839
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 763
MalI GATC 4 cut(s) 294, 344, 354, 615
MboI GATC 4 cut(s) 292, 342, 352, 613
MboII GAAGA 6 cut(s) 244, 384, 725, 803, 898, 927
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 252, 426
MluCI AATT 7 cut(s) 85, 184, 222, 264, 346, 511, 674
Mly113I GGCGCC 1 cut(s) 834
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 1 cut(s) 913
MnlI CCTC 7 cut(s) 65, 154, 184, 361, 816, 909, 958
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 4 cut(s) 381, 492, 522, 639
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 4 cut(s) 80, 106, 731, 902
MspR9I CCNGG 2 cut(s) 61, 398
MvaI CCWGG 2 cut(s) 61, 398
MvnI CGCG 2 cut(s) 216, 598
MwoI GCNNNNNNNGC 2 cut(s) 256, 595
NaeI GCCGGC 1 cut(s) 81
NarI GGCGCC 1 cut(s) 834
NdeII GATC 4 cut(s) 292, 342, 352, 613
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 6 cut(s) 100, 565, 628, 748, 753, 830
NlaIV GGNNCC 2 cut(s) 104, 835
NspI RCATGY 1 cut(s) 748
PcsI WCGNNNNNNNCGW 1 cut(s) 879
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 4 cut(s) 202, 429, 496, 667
PflMI CCANNNNNTGG 1 cut(s) 157
PinAI ACCGGT 1 cut(s) 730
PkrI GCNGC 5 cut(s) 242, 261, 591, 631, 955
PleI GAGTC 1 cut(s) 379
PluTI GGCGCC 1 cut(s) 837
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 59, 396
PspGI CCWGG 2 cut(s) 59, 396
PspN4I GGNNCC 2 cut(s) 104, 835
PspPI GGNCC 2 cut(s) 148, 218
PsrI GAACNNNNNNTAC 2 cut(s) 586, 618
PsuI RGATCY 1 cut(s) 352
RsaI GTAC 2 cut(s) 5, 940
RsaNI GTAC 2 cut(s) 4, 939
SaqAI TTAA 4 cut(s) 381, 492, 522, 639
SatI GCNGC 5 cut(s) 241, 260, 590, 630, 954
Sau3AI GATC 4 cut(s) 292, 342, 352, 613
Sau96I GGNCC 2 cut(s) 148, 218
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 2 cut(s) 61, 398
SduI GDGCHC 2 cut(s) 252, 426
SetI ASST 7 cut(s) 65, 138, 462, 576, 690, 778, 871
SexAI ACCWGGT 1 cut(s) 59
SfoI GGCGCC 1 cut(s) 835
SinI GGWCC 1 cut(s) 148
SmlI CTYRAG 3 cut(s) 491, 656, 755
SmoI CTYRAG 3 cut(s) 491, 656, 755
Sse9I AATT 7 cut(s) 85, 184, 222, 264, 346, 511, 674
SsiI CCGC 4 cut(s) 100, 216, 598, 951
SspDI GGCGCC 1 cut(s) 833
SspMI CTAG 1 cut(s) 839
StyD4I CCNGG 2 cut(s) 59, 396
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 2 cut(s) 580, 943
TaiI ACGT 1 cut(s) 138
TaqI TCGA 8 cut(s) 110, 129, 198, 341, 474, 672, 735, 873
TasI AATT 7 cut(s) 85, 184, 222, 264, 346, 511, 674
TfiI GAWTC 4 cut(s) 202, 429, 496, 667
Tru1I TTAA 4 cut(s) 381, 492, 522, 639
Tru9I TTAA 4 cut(s) 381, 492, 522, 639
TscAI CASTG 2 cut(s) 455, 669
TseI GCWGC 5 cut(s) 240, 259, 589, 629, 953
TspDTI ATGAA 4 cut(s) 215, 550, 824, 843
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 2 cut(s) 455, 669
Van91I CCANNNNNTGG 1 cut(s) 157
Vha464I CTTAAG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 683
XapI RAATTY 2 cut(s) 85, 674
XceI RCATGY 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 374
XspI CTAG 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.