Rw7G014730

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000349
Physical Location & Seq
Forward (+)
14422039 .. 14427013
4975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014730.1

Sequence Viewer

Length: 1305 bp
ATGAAAGAGACCCCAATGACACCACCTCTTCCAAACTCTAATCAGAACCTAAACGACCTACCGCAGGAAGTCCTCGTCTATATCTTGTCCTTCCTCCCCACAATAGAGGCGATCCAGACCTCTCTCATTTCCAGGAAATGGCGAAACCTCTGGTTCTTTCTCACCTCTTTCAACTTCTCCTTCGAAACCTGCTTTCCTTACTTCGATAATCCTCTTCCTTTTTATACTAGCCGGATGACTCGACCCTTCGCCAACTTTGTGGATCGCGCTCTCACCACTCGTCCCGCCGATCCTGCCGTCCATTCCTTCCGTCTATCATTCATCTACGACTCCGATTACAAAGTTCGTGTCGAGTCTTGGATACGCACCGCCGTGACGCACCTCCAAGCCCGTGAGCTGCACCTCGACTTCTATATCGACTATGACTATGACTTCTCTGTTTACAAGTATGAATTCTTCTTCTCAGAGCTGAGAAATGGCTGCGTACAAGTATTGAAACTCACCAGTTGTAATATTACACTGCCGGACGACATGGATCTTGGCTCGCTGAGATCCATTTTTCTCTATCGGGTTAATATAGAAGACGAGGAGGTGCGAAAATTGTTTTCGGGGTGTCCAAATCTCGAGCATTTTGAGCTCCGAAACTGTCTGGGGGTGATGGGTTTGTTTATACGTAGCACCAAGCTTAAGGAGCTTGCTCTTGGATTTGTTCCTAATCTAGCTGCATTCAGCTGGAACACTACAGAGATCGAGATTGATTGCCCTAACCTTCTTTCATTAAGTTTCACTGAATGCGACTTTAACCATTTTGTGCTTAAAAAAGCTGCACTAGTCGAGCTTTCTATTCATCTTTCAGTGCTAGACTCTGAGGACTATCATTTATGGAGGAAGACTGTGCGGCTACTCCTGCAAGCACCTAATGTTAAGAAGCTTAAGGTGCCAAATTTGTGGATTATGCTTCTGACATCAAGGGATCTCTTCCCTACAAGTTTTAAGCTCCAGAGTCTCAACCATTTAGAGCTAGGGACGGGTTATAGTCAAAAGGATCTGCTTGGTATGGTTGCACTGCTTGGTCATTGTCCCAATCTCGAGACAATGGTTCTTTATCACCTTCGCACGAGGAATGAAGATGAGAAGCTACCAGAAGAAGTCTTATGTAAACTAGTTAAGCTCACAATGCCAAGGATCAAGCTACTTAAGATGAGAGGGTATTTTAGAACACAAGATGTCTTCAAGTTTGTGACTATGTTGAGAAGGCAAGGAGTAGTGGCGGAGAAGATCGTAGTTGATTCTCCTCAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

434

Amino Acids

50.7

Weight (kDa)

7.13

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 17 - 51 1.6e-08 F-box domain
F-box-like PF12937 17 - 50 1.4e-06 F-box-like
LRR_At1g61320_AtMIF1 PF23622 103 - 380 1.9e-21 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 118 - 235 6.4e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G43415
fragaria_vesca FvH4_1g28610 FvH4_6g43940 FvH4_6g43940 FvH4_6g43940 FvH4_6g43950 FvH4_6g43960 FvH4_6g43970 FvH4_6g43990 FvH4_6g44000 FvH4_7g14510
malus_domestica MD09G1101800.v1.1 MD17G1089200.v1.1 MD17G1089300.v1.1 MD17G1089400.v1.1 MD17G1089500.v1.1 MD17G1089600.v1.1
prunus_persica Prupe.3G223900_v2.0.a1 Prupe.3G223900_v2.0.a1 Prupe.3G224000_v2.0.a1 Prupe.3G224100_v2.0.a1 Prupe.3G224200_v2.0.a1
pyrus_communis pycom09g02550 pycom17g02610 pycom17g08610 pycom17g08620 pycom17g08630 pycom17g08640 pycom17g08670
rosa_chinensis RchiOBHm_Chr2g0160711 RchiOBHm_Chr2g0160721 RchiOBHm_Chr2g0160731 RchiOBHm_Chr2g0160741 RchiOBHm_Chr4g0433831 RchiOBHm_Chr7g0197991
rosa_laevigata RLG00000001614 RLG00000002340 RLG00000003895 RLG00000005223 RLG00000021235 RLG00000021236 RLG00000021237 RLG00000021238 RLG00000028664 RLG00000029542 RLG00000029654 RLG00000035034
rosa_multiflora Rmu_co8177938.1_g000001 Rmu_co8474907.1_g000002 Rmu_sc0000064.1_g000011 Rmu_sc0000221.1_g000038 Rmu_sc0002773.1_g000001 Rmu_sc0002773.1_g000004 Rmu_sc0002773.1_g000006 Rmu_sc0002773.1_g000008 Rmu_sc0003465.1_g000005 Rmu_sc0006325.1_g000010 Rmu_sc0024525.1_g000002 Rmu_sc0030848.1_g000001 Rmu_sc0030848.1_g000002 Rmu_sc0030848.1_g000004
rosa_roxburghii Rroxscaffold_2G00089410 Rroxscaffold_2G00089420 Rroxscaffold_2G00089430 Rroxscaffold_2G00089450 Rroxscaffold_2G00089460 Rroxscaffold_3G00258030 Rroxscaffold_5G00351500
rosa_rugosa Rorug02G0485200 Rorug02G0485400 Rorug02G0485500 Rorug02G0485600 Rorug06G0149200
rosa_samantha Rh1AG011000 Rh2AG501800 Rh2AG550200 Rh2AG550300 Rh2AG550400 Rh2AG550500 Rh2BG217000 Rh2BG564800 Rh2BG564900 Rh2BG565000 Rh2BG565100 Rh2BG607100 Rh2CG487700 Rh2CG534600 Rh2CG534700 Rh2CG534800 Rh2CG534900 Rh2DG573500 Rh2DG573600 Rh2DG574000 Rh2DG574100 Rh3CG290600 Rh7AG168600 Rh7BG171600 Rh7BG171700 Rh7CG178100 Rh7DG170700 Rh7DG170900
rosa_wichuraiana Rw2G022070 Rw2G045590 Rw2G045600 Rw2G045610 Rw2G045620 Rw3G021600 Rw7G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 937
AccB7I CCANNNNNTGG 1 cut(s) 138
AccII CGCG 1 cut(s) 267
AciI CCGC 5 cut(s) 62, 285, 369, 898, 1271
AclWI GGATC 8 cut(s) 106, 270, 284, 543, 546, 981, 1053, 1193
AcsI RAATTY 2 cut(s) 452, 943
AfaI GTAC 1 cut(s) 486
AfiI CCNNNNNNNGG 2 cut(s) 64, 138
AflII CTTAAG 3 cut(s) 686, 932, 1196
AgsI TTSAA 3 cut(s) 172, 496, 1234
AhlI ACTAGT 2 cut(s) 829, 1162
AjnI CCWGG 1 cut(s) 131
AleI CACNNNNGTG 1 cut(s) 371
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 3 cut(s) 2, 1010, 1085
AlwI GGATC 8 cut(s) 106, 270, 284, 543, 546, 981, 1053, 1193
Ama87I CYCGRG 2 cut(s) 623, 1088
ApeKI GCWGC 4 cut(s) 397, 480, 722, 824
ApoI RAATTY 2 cut(s) 452, 943
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
AspLEI GCGC 1 cut(s) 269
AsuHPI GGTGA 5 cut(s) 154, 265, 493, 667, 1100
AsuII TTCGAA 1 cut(s) 183
AvaI CYCGRG 2 cut(s) 623, 1088
BanI GGYRCC 1 cut(s) 937
BanII GRGCYC 1 cut(s) 639
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BauI CACGAG 1 cut(s) 1117
BbsI GAAGAC 3 cut(s) 588, 896, 1222
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 4 cut(s) 384, 467, 709, 811
BccI CCATC 1 cut(s) 652
BceAI ACGGC 2 cut(s) 281, 356
BciT130I CCWGG 1 cut(s) 133
BciVI GTATCC 1 cut(s) 354
BcoDI GTCTC 3 cut(s) 2, 1010, 1085
BcuI ACTAGT 2 cut(s) 829, 1162
BfaI CTAG 6 cut(s) 228, 719, 830, 860, 1022, 1163
BfmI CTRYAG 1 cut(s) 741
BfrI CTTAAG 3 cut(s) 686, 932, 1196
BfuAI ACCTGC 1 cut(s) 197
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 5 cut(s) 398, 481, 723, 825, 899
BlsI GCNGC 5 cut(s) 399, 482, 724, 826, 900
Bme1390I CCNGG 1 cut(s) 133
BmeT110I CYCGRG 2 cut(s) 623, 1088
BmiI GGNNCC 1 cut(s) 939
BmrFI CCNGG 1 cut(s) 133
BpiI GAAGAC 3 cut(s) 588, 896, 1222
BpmI CTGGAG 1 cut(s) 983
Bpu14I TTCGAA 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 674
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 1 cut(s) 1181
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 138
Bse1I ACTGG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 1181
BseGI GGATG 1 cut(s) 240
BseLI CCNNNNNNNGG 2 cut(s) 64, 138
BseMII CTCAG 4 cut(s) 461, 477, 539, 858
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 2 cut(s) 602, 1284
BseXI GCAGC 4 cut(s) 384, 467, 709, 811
BsgI GTGCAG 2 cut(s) 383, 810
Bsh1236I CGCG 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 937
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 2 cut(s) 623, 1088
BsiSI CCGG 2 cut(s) 232, 524
BslFI GGGAC 3 cut(s) 267, 1039, 1065
BslI CCNNNNNNNGG 2 cut(s) 64, 138
BsmAI GTCTC 3 cut(s) 2, 1010, 1085
BsmFI GGGAC 3 cut(s) 267, 1039, 1065
BsmI GAATGC 2 cut(s) 725, 797
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 2 cut(s) 623, 1088
Bsp119I TTCGAA 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 639
BspACI CCGC 5 cut(s) 62, 285, 369, 898, 1271
BspCNI CTCAG 4 cut(s) 462, 476, 540, 859
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 939
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 8 cut(s) 106, 270, 284, 543, 546, 981, 1053, 1193
BspT104I TTCGAA 1 cut(s) 183
BspT107I GGYRCC 1 cut(s) 937
BspTI CTTAAG 3 cut(s) 686, 932, 1196
BspTNI GGTCTC 1 cut(s) 2
BsrI ACTGG 1 cut(s) 504
BssECI CCNNGG 1 cut(s) 1181
BssSI CACGAG 1 cut(s) 1117
BssT1I CCWWGG 1 cut(s) 1181
Bst2BI CACGAG 1 cut(s) 1117
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 2 cut(s) 647, 895
Bst6I CTCTTC 3 cut(s) 33, 219, 983
BstAFI CTTAAG 3 cut(s) 686, 932, 1196
BstBAI YACGTR 1 cut(s) 674
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 545, 696, 912
BstDEI CTNAG 4 cut(s) 463, 470, 548, 867
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 269
BstMAI GTCTC 3 cut(s) 2, 1010, 1085
BstMWI GCNNNNNNNGC 6 cut(s) 293, 634, 691, 907, 937, 1177
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 1 cut(s) 741
BstSNI TACGTA 1 cut(s) 674
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 4 cut(s) 384, 467, 709, 811
BstV2I GAAGAC 3 cut(s) 588, 896, 1222
BstX2I RGATCY 4 cut(s) 535, 551, 973, 1045
BstXI CCANNNNNNTGG 2 cut(s) 259, 948
BstYI RGATCY 4 cut(s) 535, 551, 973, 1045
BsuI GTATCC 1 cut(s) 354
BtsCI GGATG 1 cut(s) 240
BtsI GCAGTG 2 cut(s) 518, 1064
BtsIMutI CAGTG 4 cut(s) 518, 786, 861, 1064
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 3 cut(s) 545, 696, 912
CfoI GCGC 1 cut(s) 269
CseI GACGC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 485
CviAII CATG 1 cut(s) 532
CviQI GTAC 1 cut(s) 485
DdeI CTNAG 4 cut(s) 463, 470, 548, 867
Eam1104I CTCTTC 3 cut(s) 33, 219, 983
EarI CTCTTC 3 cut(s) 33, 219, 983
EciI GGCGGA 1 cut(s) 1286
Ecl136II GAGCTC 1 cut(s) 637
Eco105I TACGTA 1 cut(s) 674
Eco130I CCWWGG 1 cut(s) 1181
Eco24I GRGCYC 1 cut(s) 639
Eco31I GGTCTC 1 cut(s) 2
Eco53kI GAGCTC 1 cut(s) 637
Eco88I CYCGRG 2 cut(s) 623, 1088
EcoICRI GAGCTC 1 cut(s) 637
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 1181
EcoT38I GRGCYC 1 cut(s) 639
ErhI CCWWGG 1 cut(s) 1181
FaeI CATG 1 cut(s) 535
FaqI GGGAC 3 cut(s) 267, 1039, 1065
FatI CATG 1 cut(s) 531
FauI CCCGC 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 398, 481, 723, 825, 899
FokI GGATG 1 cut(s) 247
FriOI GRGCYC 1 cut(s) 639
Fsp4HI GCNGC 5 cut(s) 398, 481, 723, 825, 899
FspBI CTAG 6 cut(s) 228, 719, 830, 860, 1022, 1163
GlaI GCGC 1 cut(s) 268
GluI GCNGC 5 cut(s) 398, 481, 723, 825, 899
GsuI CTGGAG 1 cut(s) 983
HapII CCGG 2 cut(s) 232, 524
HgaI GACGC 1 cut(s) 385
HhaI GCGC 1 cut(s) 269
Hin1II CATG 1 cut(s) 535
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HindIII AAGCTT 2 cut(s) 683, 929
HinfI GANTC 6 cut(s) 238, 329, 353, 863, 1003, 1289
HpaII CCGG 2 cut(s) 232, 524
HphI GGTGA 5 cut(s) 154, 265, 493, 667, 1100
Hpy166II GTNNAC 2 cut(s) 442, 1160
Hpy188I TCNGA 6 cut(s) 45, 334, 466, 641, 868, 963
Hpy188III TCNNGA 6 cut(s) 115, 623, 751, 1000, 1088, 1090
Hpy8I GTNNAC 2 cut(s) 442, 1160
HpyAV CCTTC 7 cut(s) 100, 190, 256, 316, 779, 1121, 1248
HpyCH4III ACNGT 2 cut(s) 647, 895
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 5 cut(s) 400, 725, 827, 910, 1064
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 634, 691, 907, 937, 1177
HpyF3I CTNAG 4 cut(s) 463, 470, 548, 867
HpySE526I ACGT 1 cut(s) 673
Hsp92II CATG 1 cut(s) 535
HspAI GCGC 1 cut(s) 267
LmnI GCTCC 3 cut(s) 642, 691, 1002
Lsp1109I GCAGC 4 cut(s) 384, 467, 709, 811
MaeI CTAG 6 cut(s) 228, 719, 830, 860, 1022, 1163
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 2 cut(s) 373, 1240
MflI RGATCY 4 cut(s) 535, 551, 973, 1045
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 3 cut(s) 452, 599, 943
MlyI GAGTC 5 cut(s) 232, 323, 362, 857, 1012
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 732
MspCI CTTAAG 3 cut(s) 686, 932, 1196
MspI CCGG 2 cut(s) 232, 524
MspR9I CCNGG 1 cut(s) 133
Mva1269I GAATGC 2 cut(s) 725, 797
MvaI CCWGG 1 cut(s) 133
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 6 cut(s) 293, 634, 691, 907, 937, 1177
NlaIII CATG 1 cut(s) 535
NlaIV GGNNCC 1 cut(s) 939
NmuCI GTSAC 2 cut(s) 373, 1240
NspV TTCGAA 1 cut(s) 183
OliI CACNNNNGTG 1 cut(s) 371
PaeR7I CTCGAG 2 cut(s) 623, 1088
PctI GAATGC 2 cut(s) 725, 797
PfeI GAWTC 1 cut(s) 1289
PflMI CCANNNNNTGG 1 cut(s) 138
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 5 cut(s) 399, 482, 724, 826, 900
PleI GAGTC 5 cut(s) 232, 323, 361, 857, 1011
PpsI GAGTC 5 cut(s) 232, 323, 361, 857, 1011
Ppu21I YACGTR 1 cut(s) 674
Psp124BI GAGCTC 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 939
PsuI RGATCY 4 cut(s) 535, 551, 973, 1045
PvuII CAGCTG 1 cut(s) 732
RsaI GTAC 1 cut(s) 486
RsaNI GTAC 1 cut(s) 485
RseI CAYNNNNRTG 1 cut(s) 371
SacI GAGCTC 1 cut(s) 639
SatI GCNGC 5 cut(s) 398, 481, 723, 825, 899
SchI GAGTC 5 cut(s) 232, 323, 362, 857, 1012
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 639
SfcI CTRYAG 1 cut(s) 741
Sfr274I CTCGAG 2 cut(s) 623, 1088
SfuI TTCGAA 1 cut(s) 183
SlaI CTCGAG 2 cut(s) 623, 1088
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 5 cut(s) 623, 686, 932, 1088, 1196
SmoI CTYRAG 5 cut(s) 623, 686, 932, 1088, 1196
SnaBI TACGTA 1 cut(s) 674
SpeI ACTAGT 2 cut(s) 829, 1162
Sse9I AATT 3 cut(s) 452, 599, 943
SsiI CCGC 5 cut(s) 62, 285, 369, 898, 1271
SspI AATATT 1 cut(s) 514
SspMI CTAG 6 cut(s) 228, 719, 830, 860, 1022, 1163
SstI GAGCTC 1 cut(s) 639
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 1 cut(s) 1181
TaaI ACNGT 2 cut(s) 647, 895
TaiI ACGT 1 cut(s) 676
TasI AATT 3 cut(s) 452, 599, 943
TauI GCSGC 1 cut(s) 901
TfiI GAWTC 1 cut(s) 1289
TscAI CASTG 4 cut(s) 525, 793, 861, 1071
TseFI GTSAC 2 cut(s) 373, 1240
TseI GCWGC 4 cut(s) 397, 480, 722, 824
Tsp45I GTSAC 2 cut(s) 373, 1240
TspDTI ATGAA 6 cut(s) 17, 310, 465, 765, 836, 1140
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 4 cut(s) 525, 793, 861, 1071
Van91I CCANNNNNTGG 1 cut(s) 138
Vha464I CTTAAG 3 cut(s) 686, 932, 1196
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 2 cut(s) 452, 943
XhoI CTCGAG 2 cut(s) 623, 1088
XspI CTAG 6 cut(s) 228, 719, 830, 860, 1022, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.