Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 132, 207 |
| AcuI | CTGAAG | 4 cut(s) | 412, 642, 656, 662 |
| AfaI | GTAC | 4 cut(s) | 369, 572, 828, 1040 |
| AfeI | AGCGCT | 1 cut(s) | 627 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 839 |
| AgsI | TTSAA | 4 cut(s) | 236, 652, 797, 1120 |
| AjnI | CCWGG | 3 cut(s) | 120, 222, 838 |
| AleI | CACNNNNGTG | 1 cut(s) | 395 |
| AluBI | AGCT | 4 cut(s) | 202, 409, 877, 1123 |
| AluI | AGCT | 4 cut(s) | 202, 409, 877, 1123 |
| Alw21I | GWGCWC | 1 cut(s) | 472 |
| Alw26I | GTCTC | 3 cut(s) | 290, 549, 1040 |
| Alw44I | GTGCAC | 1 cut(s) | 468 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 616 |
| Aor51HI | AGCGCT | 1 cut(s) | 627 |
| AoxI | GGCC | 3 cut(s) | 97, 229, 1087 |
| ApaLI | GTGCAC | 1 cut(s) | 468 |
| ApoI | RAATTY | 2 cut(s) | 132, 207 |
| AspLEI | GCGC | 2 cut(s) | 628, 734 |
| AspS9I | GGNCC | 3 cut(s) | 935, 998, 1098 |
| AsuHPI | GGTGA | 1 cut(s) | 409 |
| AvaII | GGWCC | 3 cut(s) | 935, 998, 1098 |
| BaeGI | GKGCMC | 1 cut(s) | 472 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 359, 392 |
| BanII | GRGCYC | 1 cut(s) | 847 |
| BauI | CACGAG | 1 cut(s) | 417 |
| BbsI | GAAGAC | 3 cut(s) | 540, 785, 1029 |
| Bbv12I | GWGCWC | 1 cut(s) | 472 |
| BccI | CCATC | 2 cut(s) | 170, 1088 |
| BciT130I | CCWGG | 3 cut(s) | 122, 224, 840 |
| BciVI | GTATCC | 1 cut(s) | 168 |
| BcoDI | GTCTC | 3 cut(s) | 290, 549, 1040 |
| BfaI | CTAG | 1 cut(s) | 848 |
| BfmI | CTRYAG | 1 cut(s) | 872 |
| BfoI | RGCGCY | 1 cut(s) | 629 |
| BfuI | GTATCC | 1 cut(s) | 168 |
| BglII | AGATCT | 1 cut(s) | 865 |
| BmcAI | AGTACT | 1 cut(s) | 572 |
| Bme1390I | CCNGG | 3 cut(s) | 122, 224, 840 |
| Bme18I | GGWCC | 3 cut(s) | 935, 998, 1098 |
| BmgT120I | GGNCC | 3 cut(s) | 935, 998, 1098 |
| BmiI | GGNNCC | 5 cut(s) | 27, 126, 513, 1000, 1099 |
| BmrFI | CCNGG | 3 cut(s) | 122, 224, 840 |
| BmsI | GCATC | 2 cut(s) | 97, 299 |
| BpiI | GAAGAC | 3 cut(s) | 540, 785, 1029 |
| Bpu10I | CCTNAGC | 1 cut(s) | 525 |
| BsaAI | YACGTR | 1 cut(s) | 371 |
| BsaBI | GATNNNNATC | 2 cut(s) | 156, 246 |
| BsaJI | CCNNGG | 2 cut(s) | 120, 839 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 839 |
| Bse118I | RCCGGY | 1 cut(s) | 932 |
| Bse1I | ACTGG | 4 cut(s) | 146, 212, 610, 973 |
| Bse3DI | GCAATG | 1 cut(s) | 980 |
| Bse8I | GATNNNNATC | 2 cut(s) | 156, 246 |
| BseBI | CCWGG | 3 cut(s) | 122, 224, 840 |
| BseDI | CCNNGG | 2 cut(s) | 120, 839 |
| BseJI | GATNNNNATC | 2 cut(s) | 156, 246 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 839 |
| BseMI | GCAATG | 1 cut(s) | 980 |
| BseMII | CTCAG | 2 cut(s) | 217, 384 |
| BseNI | ACTGG | 4 cut(s) | 146, 212, 610, 973 |
| BseSI | GKGCMC | 1 cut(s) | 472 |
| BseYI | CCCAGC | 2 cut(s) | 110, 198 |
| BshFI | GGCC | 3 cut(s) | 99, 231, 1089 |
| BsiHKAI | GWGCWC | 1 cut(s) | 472 |
| BsiSI | CCGG | 1 cut(s) | 933 |
| BslFI | GGGAC | 2 cut(s) | 503, 1111 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 839 |
| BsmAI | GTCTC | 3 cut(s) | 290, 549, 1040 |
| BsmFI | GGGAC | 2 cut(s) | 503, 1111 |
| BsnI | GGCC | 3 cut(s) | 99, 231, 1089 |
| Bsp1286I | GDGCHC | 2 cut(s) | 472, 847 |
| Bsp143I | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| BspANI | GGCC | 3 cut(s) | 99, 231, 1089 |
| BspCNI | CTCAG | 2 cut(s) | 216, 385 |
| BspLI | GGNNCC | 5 cut(s) | 27, 126, 513, 1000, 1099 |
| BsrDI | GCAATG | 1 cut(s) | 980 |
| BsrFI | RCCGGY | 1 cut(s) | 932 |
| BsrI | ACTGG | 4 cut(s) | 146, 212, 610, 973 |
| BssAI | RCCGGY | 1 cut(s) | 932 |
| BssECI | CCNNGG | 2 cut(s) | 120, 839 |
| BssMI | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| BssSI | CACGAG | 1 cut(s) | 417 |
| Bst2BI | CACGAG | 1 cut(s) | 417 |
| Bst2UI | CCWGG | 3 cut(s) | 122, 224, 840 |
| Bst4CI | ACNGT | 2 cut(s) | 766, 1054 |
| Bst6I | CTCTTC | 1 cut(s) | 345 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 929 |
| BstBAI | YACGTR | 1 cut(s) | 371 |
| BstC8I | GCNNGC | 3 cut(s) | 736, 740, 744 |
| BstDEI | CTNAG | 3 cut(s) | 203, 393, 525 |
| BstH2I | RGCGCY | 1 cut(s) | 629 |
| BstHHI | GCGC | 2 cut(s) | 628, 734 |
| BstKTI | GATC | 5 cut(s) | 154, 441, 460, 781, 868 |
| BstMAI | GTCTC | 3 cut(s) | 290, 549, 1040 |
| BstMBI | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 929 |
| BstNI | CCWGG | 3 cut(s) | 122, 224, 840 |
| BstSCI | CCNGG | 3 cut(s) | 120, 222, 838 |
| BstSFI | CTRYAG | 1 cut(s) | 872 |
| BstSLI | GKGCMC | 1 cut(s) | 472 |
| BstV2I | GAAGAC | 3 cut(s) | 540, 785, 1029 |
| BstX2I | RGATCY | 1 cut(s) | 865 |
| BstYI | RGATCY | 1 cut(s) | 865 |
| BsuI | GTATCC | 1 cut(s) | 168 |
| BsuRI | GGCC | 3 cut(s) | 99, 231, 1089 |
| BtsIMutI | CAGTG | 2 cut(s) | 390, 966 |
| Cac8I | GCNNGC | 3 cut(s) | 736, 740, 744 |
| CaiI | CAGNNNCTG | 1 cut(s) | 616 |
| CfoI | GCGC | 2 cut(s) | 628, 734 |
| Cfr10I | RCCGGY | 1 cut(s) | 932 |
| Cfr13I | GGNCC | 3 cut(s) | 935, 998, 1098 |
| Csp6I | GTAC | 4 cut(s) | 368, 571, 827, 1039 |
| CviAII | CATG | 2 cut(s) | 326, 658 |
| CviQI | GTAC | 4 cut(s) | 368, 571, 827, 1039 |
| DdeI | CTNAG | 3 cut(s) | 203, 393, 525 |
| DpnI | GATC | 5 cut(s) | 153, 440, 459, 780, 867 |
| DpnII | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| Eam1104I | CTCTTC | 1 cut(s) | 345 |
| EarI | CTCTTC | 1 cut(s) | 345 |
| Eco147I | AGGCCT | 3 cut(s) | 99, 231, 1089 |
| Eco24I | GRGCYC | 1 cut(s) | 847 |
| Eco47I | GGWCC | 3 cut(s) | 935, 998, 1098 |
| Eco47III | AGCGCT | 1 cut(s) | 627 |
| Eco57I | CTGAAG | 4 cut(s) | 412, 642, 656, 662 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1098 |
| EcoRI | GAATTC | 1 cut(s) | 207 |
| EcoRII | CCWGG | 3 cut(s) | 120, 222, 838 |
| EcoT38I | GRGCYC | 1 cut(s) | 847 |
| FaeI | CATG | 2 cut(s) | 329, 661 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1014, 1046 |
| FaqI | GGGAC | 2 cut(s) | 503, 1111 |
| FatI | CATG | 2 cut(s) | 325, 657 |
| FriOI | GRGCYC | 1 cut(s) | 847 |
| FspBI | CTAG | 1 cut(s) | 848 |
| GlaI | GCGC | 2 cut(s) | 627, 733 |
| GsaI | CCCAGC | 2 cut(s) | 114, 202 |
| HaeII | RGCGCY | 1 cut(s) | 629 |
| HaeIII | GGCC | 3 cut(s) | 99, 231, 1089 |
| HapII | CCGG | 1 cut(s) | 933 |
| HhaI | GCGC | 2 cut(s) | 628, 734 |
| Hin1II | CATG | 2 cut(s) | 329, 661 |
| Hin6I | GCGC | 2 cut(s) | 626, 732 |
| HinP1I | GCGC | 2 cut(s) | 626, 732 |
| HincII | GTYRAC | 2 cut(s) | 723, 856 |
| HindII | GTYRAC | 2 cut(s) | 723, 856 |
| HinfI | GANTC | 3 cut(s) | 179, 491, 894 |
| HpaI | GTTAAC | 2 cut(s) | 723, 856 |
| HpaII | CCGG | 1 cut(s) | 933 |
| HphI | GGTGA | 1 cut(s) | 409 |
| Hpy166II | GTNNAC | 4 cut(s) | 470, 710, 723, 856 |
| Hpy188I | TCNGA | 8 cut(s) | 184, 206, 295, 431, 622, 810, 1021, 1127 |
| Hpy188III | TCNNGA | 2 cut(s) | 419, 898 |
| Hpy8I | GTNNAC | 4 cut(s) | 470, 710, 723, 856 |
| HpyAV | CCTTC | 3 cut(s) | 80, 221, 1051 |
| HpyCH4III | ACNGT | 2 cut(s) | 766, 1054 |
| HpyCH4IV | ACGT | 1 cut(s) | 370 |
| HpyCH4V | TGCA | 4 cut(s) | 470, 552, 690, 775 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 929 |
| HpyF3I | CTNAG | 3 cut(s) | 203, 393, 525 |
| HpySE526I | ACGT | 1 cut(s) | 370 |
| Hsp92II | CATG | 2 cut(s) | 329, 661 |
| HspAI | GCGC | 2 cut(s) | 626, 732 |
| KspAI | GTTAAC | 2 cut(s) | 723, 856 |
| Kzo9I | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| LmnI | GCTCC | 1 cut(s) | 517 |
| LweI | GCATC | 2 cut(s) | 97, 299 |
| MaeI | CTAG | 1 cut(s) | 848 |
| MaeII | ACGT | 1 cut(s) | 370 |
| MaeIII | GTNAC | 1 cut(s) | 644 |
| MalI | GATC | 5 cut(s) | 153, 440, 459, 780, 867 |
| MboI | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| MboII | GAAGA | 8 cut(s) | 362, 412, 545, 785, 794, 860, 1022, 1034 |
| MflI | RGATCY | 1 cut(s) | 865 |
| MhlI | GDGCHC | 2 cut(s) | 472, 847 |
| MluCI | AATT | 6 cut(s) | 40, 132, 207, 315, 481, 691 |
| MlyI | GAGTC | 1 cut(s) | 485 |
| MmeI | TCCRAC | 1 cut(s) | 275 |
| MnlI | CCTC | 5 cut(s) | 280, 388, 480, 531, 1111 |
| MseI | TTAA | 6 cut(s) | 93, 722, 855, 1005, 1080, 1109 |
| MslI | CAYNNNNRTG | 4 cut(s) | 395, 547, 651, 786 |
| MspI | CCGG | 1 cut(s) | 933 |
| MspR9I | CCNGG | 3 cut(s) | 122, 224, 840 |
| MvaI | CCWGG | 3 cut(s) | 122, 224, 840 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 929 |
| NdeII | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| NlaIII | CATG | 2 cut(s) | 329, 661 |
| NlaIV | GGNNCC | 5 cut(s) | 27, 126, 513, 1000, 1099 |
| NmuCI | GTSAC | 1 cut(s) | 644 |
| OliI | CACNNNNGTG | 1 cut(s) | 395 |
| PceI | AGGCCT | 3 cut(s) | 99, 231, 1089 |
| PfeI | GAWTC | 2 cut(s) | 179, 894 |
| PleI | GAGTC | 1 cut(s) | 485 |
| PpsI | GAGTC | 1 cut(s) | 485 |
| Ppu21I | YACGTR | 1 cut(s) | 371 |
| PpuMI | RGGWCCY | 1 cut(s) | 1098 |
| Psp5II | RGGWCCY | 1 cut(s) | 1098 |
| Psp6I | CCWGG | 3 cut(s) | 120, 222, 838 |
| PspFI | CCCAGC | 2 cut(s) | 110, 198 |
| PspGI | CCWGG | 3 cut(s) | 120, 222, 838 |
| PspN4I | GGNNCC | 5 cut(s) | 27, 126, 513, 1000, 1099 |
| PspPI | GGNCC | 3 cut(s) | 935, 998, 1098 |
| PspPPI | RGGWCCY | 1 cut(s) | 1098 |
| PstNI | CAGNNNCTG | 1 cut(s) | 616 |
| PsuI | RGATCY | 1 cut(s) | 865 |
| RsaI | GTAC | 4 cut(s) | 369, 572, 828, 1040 |
| RsaNI | GTAC | 4 cut(s) | 368, 571, 827, 1039 |
| RseI | CAYNNNNRTG | 4 cut(s) | 395, 547, 651, 786 |
| SaqAI | TTAA | 6 cut(s) | 93, 722, 855, 1005, 1080, 1109 |
| Sau3AI | GATC | 5 cut(s) | 151, 438, 457, 778, 865 |
| Sau96I | GGNCC | 3 cut(s) | 935, 998, 1098 |
| ScaI | AGTACT | 1 cut(s) | 572 |
| SchI | GAGTC | 1 cut(s) | 485 |
| ScrFI | CCNGG | 3 cut(s) | 122, 224, 840 |
| SduI | GDGCHC | 2 cut(s) | 472, 847 |
| SfaNI | GCATC | 2 cut(s) | 97, 299 |
| SfcI | CTRYAG | 1 cut(s) | 872 |
| SinI | GGWCC | 3 cut(s) | 935, 998, 1098 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 395, 547, 651, 786 |
| Sse9I | AATT | 6 cut(s) | 40, 132, 207, 315, 481, 691 |
| SseBI | AGGCCT | 3 cut(s) | 99, 231, 1089 |
| SspMI | CTAG | 1 cut(s) | 848 |
| StuI | AGGCCT | 3 cut(s) | 99, 231, 1089 |
| StyD4I | CCNGG | 3 cut(s) | 120, 222, 838 |
| TaaI | ACNGT | 2 cut(s) | 766, 1054 |
| TaiI | ACGT | 1 cut(s) | 373 |
| TaqI | TCGA | 1 cut(s) | 456 |
| TasI | AATT | 6 cut(s) | 40, 132, 207, 315, 481, 691 |
| TatI | WGTACW | 3 cut(s) | 570, 826, 1038 |
| TfiI | GAWTC | 2 cut(s) | 179, 894 |
| Tru1I | TTAA | 6 cut(s) | 93, 722, 855, 1005, 1080, 1109 |
| Tru9I | TTAA | 6 cut(s) | 93, 722, 855, 1005, 1080, 1109 |
| TscAI | CASTG | 2 cut(s) | 397, 973 |
| TseFI | GTSAC | 1 cut(s) | 644 |
| Tsp45I | GTSAC | 1 cut(s) | 644 |
| TspDTI | ATGAA | 3 cut(s) | 128, 602, 794 |
| TspRI | CASTG | 2 cut(s) | 397, 973 |
| VneI | GTGCAC | 1 cut(s) | 468 |
| VpaK11BI | GGWCC | 3 cut(s) | 935, 998, 1098 |
| XapI | RAATTY | 2 cut(s) | 132, 207 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 480 |
| XspI | CTAG | 1 cut(s) | 848 |
| ZrmI | AGTACT | 1 cut(s) | 572 |