Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 952 |
| AccI | GTMKAC | 1 cut(s) | 196 |
| AccIII | TCCGGA | 1 cut(s) | 490 |
| AciI | CCGC | 1 cut(s) | 262 |
| AclWI | GGATC | 6 cut(s) | 131, 216, 241, 392, 405, 497 |
| AcoI | YGGCCR | 2 cut(s) | 15, 178 |
| AcsI | RAATTY | 1 cut(s) | 841 |
| AcuI | CTGAAG | 2 cut(s) | 45, 755 |
| AfaI | GTAC | 4 cut(s) | 386, 440, 561, 974 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 164, 815 |
| AgsI | TTSAA | 3 cut(s) | 587, 752, 1099 |
| AjnI | CCWGG | 1 cut(s) | 814 |
| AluBI | AGCT | 6 cut(s) | 43, 68, 361, 429, 697, 821 |
| AluI | AGCT | 6 cut(s) | 43, 68, 361, 429, 697, 821 |
| Alw21I | GWGCWC | 2 cut(s) | 699, 823 |
| AlwI | GGATC | 6 cut(s) | 131, 216, 241, 392, 405, 497 |
| Ama87I | CYCGRG | 1 cut(s) | 335 |
| Aor13HI | TCCGGA | 1 cut(s) | 490 |
| AoxI | GGCC | 4 cut(s) | 15, 48, 178, 233 |
| ApeKI | GCWGC | 3 cut(s) | 266, 361, 455 |
| ApoI | RAATTY | 1 cut(s) | 841 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 57, 69, 89, 101 |
| AspS9I | GGNCC | 2 cut(s) | 911, 1078 |
| AsuC2I | CCSGG | 1 cut(s) | 1082 |
| AsuHPI | GGTGA | 4 cut(s) | 91, 227, 256, 385 |
| AvaI | CYCGRG | 1 cut(s) | 335 |
| AvaII | GGWCC | 2 cut(s) | 911, 1078 |
| BalI | TGGCCA | 2 cut(s) | 17, 180 |
| BamHI | GGATCC | 1 cut(s) | 397 |
| BanII | GRGCYC | 3 cut(s) | 498, 699, 823 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 368, 400, 401, 433 |
| Bbv12I | GWGCWC | 2 cut(s) | 699, 823 |
| BbvCI | CCTCAGC | 1 cut(s) | 142 |
| BbvI | GCAGC | 3 cut(s) | 253, 348, 442 |
| BccI | CCATC | 4 cut(s) | 86, 118, 630, 719 |
| BciT130I | CCWGG | 1 cut(s) | 816 |
| BciVI | GTATCC | 1 cut(s) | 300 |
| BclI | TGATCA | 1 cut(s) | 883 |
| BcnI | CCSGG | 1 cut(s) | 1082 |
| BfaI | CTAG | 2 cut(s) | 393, 663 |
| BfmI | CTRYAG | 1 cut(s) | 267 |
| BfuAI | ACCTGC | 1 cut(s) | 952 |
| BfuI | GTATCC | 1 cut(s) | 300 |
| BisI | GCNGC | 3 cut(s) | 267, 362, 456 |
| BlsI | GCNGC | 3 cut(s) | 268, 363, 457 |
| BmcAI | AGTACT | 1 cut(s) | 386 |
| Bme1390I | CCNGG | 2 cut(s) | 816, 1082 |
| Bme18I | GGWCC | 2 cut(s) | 911, 1078 |
| BmeT110I | CYCGRG | 1 cut(s) | 335 |
| BmgT120I | GGNCC | 2 cut(s) | 911, 1078 |
| BmiI | GGNNCC | 4 cut(s) | 399, 489, 495, 1080 |
| BmrFI | CCNGG | 2 cut(s) | 816, 1082 |
| BmsI | GCATC | 2 cut(s) | 321, 661 |
| BpmI | CTGGAG | 1 cut(s) | 837 |
| Bpu10I | CCTNAGC | 4 cut(s) | 142, 240, 687, 693 |
| BpuMI | CCSGG | 1 cut(s) | 1082 |
| BsaJI | CCNNGG | 2 cut(s) | 301, 365 |
| BsaWI | WCCGGW | 1 cut(s) | 490 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 164, 815 |
| Bse1I | ACTGG | 4 cut(s) | 181, 200, 442, 790 |
| BseAI | TCCGGA | 1 cut(s) | 490 |
| BseBI | CCWGG | 1 cut(s) | 816 |
| BseDI | CCNNGG | 2 cut(s) | 301, 365 |
| BseGI | GGATG | 3 cut(s) | 110, 153, 935 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 164, 815 |
| BseMII | CTCAG | 6 cut(s) | 156, 395, 611, 678, 684, 735 |
| BseNI | ACTGG | 4 cut(s) | 181, 200, 442, 790 |
| BseRI | GAGGAG | 1 cut(s) | 341 |
| BseXI | GCAGC | 3 cut(s) | 253, 348, 442 |
| BshFI | GGCC | 4 cut(s) | 17, 50, 180, 235 |
| BsiHKAI | GWGCWC | 2 cut(s) | 699, 823 |
| BsiHKCI | CYCGRG | 1 cut(s) | 335 |
| BsiSI | CCGG | 3 cut(s) | 259, 491, 1082 |
| BslFI | GGGAC | 2 cut(s) | 92, 1064 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 164, 815 |
| BsmFI | GGGAC | 2 cut(s) | 92, 1064 |
| BsnI | GGCC | 4 cut(s) | 17, 50, 180, 235 |
| BsoBI | CYCGRG | 1 cut(s) | 335 |
| Bsp1286I | GDGCHC | 3 cut(s) | 498, 699, 823 |
| Bsp13I | TCCGGA | 1 cut(s) | 490 |
| Bsp143I | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| Bsp19I | CCATGG | 1 cut(s) | 301 |
| BspACI | CCGC | 1 cut(s) | 262 |
| BspANI | GGCC | 4 cut(s) | 17, 50, 180, 235 |
| BspCNI | CTCAG | 6 cut(s) | 155, 394, 610, 679, 685, 734 |
| BspEI | TCCGGA | 1 cut(s) | 490 |
| BspHI | TCATGA | 1 cut(s) | 355 |
| BspLI | GGNNCC | 4 cut(s) | 399, 489, 495, 1080 |
| BspMAI | CTGCAG | 1 cut(s) | 271 |
| BspMI | ACCTGC | 1 cut(s) | 952 |
| BspPI | GGATC | 6 cut(s) | 131, 216, 241, 392, 405, 497 |
| BspQI | GCTCTTC | 1 cut(s) | 21 |
| BsrI | ACTGG | 4 cut(s) | 181, 200, 442, 790 |
| BssECI | CCNNGG | 2 cut(s) | 301, 365 |
| BssMI | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| BssNAI | GTATAC | 1 cut(s) | 197 |
| BssT1I | CCWWGG | 2 cut(s) | 301, 365 |
| Bst1107I | GTATAC | 1 cut(s) | 197 |
| Bst2UI | CCWGG | 1 cut(s) | 816 |
| Bst4CI | ACNGT | 1 cut(s) | 319 |
| Bst6I | CTCTTC | 1 cut(s) | 21 |
| BstC8I | GCNNGC | 3 cut(s) | 128, 237, 264 |
| BstDEI | CTNAG | 8 cut(s) | 142, 240, 381, 499, 597, 687, 693, 721 |
| BstDSI | CCRYGG | 1 cut(s) | 301 |
| BstF5I | GGATG | 3 cut(s) | 110, 153, 935 |
| BstKTI | GATC | 7 cut(s) | 139, 211, 249, 400, 505, 757, 886 |
| BstMBI | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 976 |
| BstNI | CCWGG | 1 cut(s) | 816 |
| BstNSI | RCATGY | 1 cut(s) | 475 |
| BstSCI | CCNGG | 2 cut(s) | 814, 1080 |
| BstSFI | CTRYAG | 1 cut(s) | 267 |
| BstV1I | GCAGC | 3 cut(s) | 253, 348, 442 |
| BstX2I | RGATCY | 4 cut(s) | 136, 208, 397, 502 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 13, 725 |
| BstYI | RGATCY | 4 cut(s) | 136, 208, 397, 502 |
| BstZ17I | GTATAC | 1 cut(s) | 197 |
| BsuI | GTATCC | 1 cut(s) | 300 |
| BsuRI | GGCC | 4 cut(s) | 17, 50, 180, 235 |
| BtgI | CCRYGG | 1 cut(s) | 301 |
| BtsCI | GGATG | 3 cut(s) | 110, 153, 935 |
| BtsIMutI | CAGTG | 2 cut(s) | 324, 797 |
| BveI | ACCTGC | 1 cut(s) | 952 |
| Cac8I | GCNNGC | 3 cut(s) | 128, 237, 264 |
| CciI | TCATGA | 1 cut(s) | 355 |
| Cfr13I | GGNCC | 2 cut(s) | 911, 1078 |
| CseI | GACGC | 2 cut(s) | 20, 352 |
| Csp6I | GTAC | 4 cut(s) | 385, 439, 560, 973 |
| CviAII | CATG | 7 cut(s) | 302, 347, 356, 472, 484, 681, 782 |
| CviQI | GTAC | 4 cut(s) | 385, 439, 560, 973 |
| DdeI | CTNAG | 8 cut(s) | 142, 240, 381, 499, 597, 687, 693, 721 |
| DpnI | GATC | 7 cut(s) | 138, 210, 248, 399, 504, 756, 885 |
| DpnII | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| EaeI | YGGCCR | 2 cut(s) | 15, 178 |
| Eam1104I | CTCTTC | 1 cut(s) | 21 |
| EarI | CTCTTC | 1 cut(s) | 21 |
| Ecl136II | GAGCTC | 2 cut(s) | 697, 821 |
| Eco130I | CCWWGG | 2 cut(s) | 301, 365 |
| Eco147I | AGGCCT | 2 cut(s) | 50, 235 |
| Eco24I | GRGCYC | 3 cut(s) | 498, 699, 823 |
| Eco32I | GATATC | 1 cut(s) | 256 |
| Eco47I | GGWCC | 2 cut(s) | 911, 1078 |
| Eco53kI | GAGCTC | 2 cut(s) | 697, 821 |
| Eco57I | CTGAAG | 2 cut(s) | 45, 755 |
| Eco88I | CYCGRG | 1 cut(s) | 335 |
| EcoICRI | GAGCTC | 2 cut(s) | 697, 821 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1078 |
| EcoRII | CCWGG | 1 cut(s) | 814 |
| EcoRV | GATATC | 1 cut(s) | 256 |
| EcoT14I | CCWWGG | 2 cut(s) | 301, 365 |
| EcoT38I | GRGCYC | 3 cut(s) | 498, 699, 823 |
| ErhI | CCWWGG | 2 cut(s) | 301, 365 |
| FaeI | CATG | 7 cut(s) | 305, 350, 359, 475, 487, 684, 785 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 639, 671 |
| FaqI | GGGAC | 2 cut(s) | 92, 1064 |
| FatI | CATG | 7 cut(s) | 301, 346, 355, 471, 483, 680, 781 |
| FauI | CCCGC | 1 cut(s) | 255 |
| FbaI | TGATCA | 1 cut(s) | 883 |
| FblI | GTMKAC | 1 cut(s) | 196 |
| Fnu4HI | GCNGC | 3 cut(s) | 267, 362, 456 |
| FokI | GGATG | 3 cut(s) | 97, 140, 942 |
| FriOI | GRGCYC | 3 cut(s) | 498, 699, 823 |
| Fsp4HI | GCNGC | 3 cut(s) | 267, 362, 456 |
| FspBI | CTAG | 2 cut(s) | 393, 663 |
| GluI | GCNGC | 3 cut(s) | 267, 362, 456 |
| GsuI | CTGGAG | 1 cut(s) | 837 |
| HaeIII | GGCC | 4 cut(s) | 17, 50, 180, 235 |
| HapII | CCGG | 3 cut(s) | 259, 491, 1082 |
| HgaI | GACGC | 2 cut(s) | 20, 352 |
| Hin1II | CATG | 7 cut(s) | 305, 350, 359, 475, 487, 684, 785 |
| HincII | GTYRAC | 3 cut(s) | 298, 832, 993 |
| HindII | GTYRAC | 3 cut(s) | 298, 832, 993 |
| HinfI | GANTC | 3 cut(s) | 59, 96, 609 |
| HpaI | GTTAAC | 1 cut(s) | 298 |
| HpaII | CCGG | 3 cut(s) | 259, 491, 1082 |
| HphI | GGTGA | 4 cut(s) | 91, 227, 256, 385 |
| Hpy166II | GTNNAC | 5 cut(s) | 197, 298, 767, 832, 993 |
| Hpy188I | TCNGA | 4 cut(s) | 608, 724, 941, 955 |
| Hpy188III | TCNNGA | 4 cut(s) | 356, 491, 686, 752 |
| Hpy8I | GTNNAC | 5 cut(s) | 197, 298, 767, 832, 993 |
| Hpy99I | CGWCG | 2 cut(s) | 346, 967 |
| HpyAV | CCTTC | 4 cut(s) | 225, 370, 403, 902 |
| HpyCH4III | ACNGT | 1 cut(s) | 319 |
| HpyCH4V | TGCA | 5 cut(s) | 126, 269, 528, 781, 850 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 976 |
| HpyF3I | CTNAG | 8 cut(s) | 142, 240, 381, 499, 597, 687, 693, 721 |
| Hsp92II | CATG | 7 cut(s) | 305, 350, 359, 475, 487, 684, 785 |
| Kpn2I | TCCGGA | 1 cut(s) | 490 |
| Ksp22I | TGATCA | 1 cut(s) | 883 |
| KspAI | GTTAAC | 1 cut(s) | 298 |
| Kzo9I | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| LguI | GCTCTTC | 1 cut(s) | 21 |
| LmnI | GCTCC | 3 cut(s) | 493, 493, 818 |
| Lsp1109I | GCAGC | 3 cut(s) | 253, 348, 442 |
| LweI | GCATC | 2 cut(s) | 321, 661 |
| MaeI | CTAG | 2 cut(s) | 393, 663 |
| MaeIII | GTNAC | 3 cut(s) | 479, 574, 665 |
| MalI | GATC | 7 cut(s) | 138, 210, 248, 399, 504, 756, 885 |
| MboI | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| MboII | GAAGA | 2 cut(s) | 38, 146 |
| MfeI | CAATTG | 1 cut(s) | 702 |
| MflI | RGATCY | 4 cut(s) | 136, 208, 397, 502 |
| MhlI | GDGCHC | 3 cut(s) | 498, 699, 823 |
| MlsI | TGGCCA | 2 cut(s) | 17, 180 |
| MluNI | TGGCCA | 2 cut(s) | 17, 180 |
| MlyI | GAGTC | 1 cut(s) | 603 |
| MmeI | TCCRAC | 3 cut(s) | 197, 303, 964 |
| Mox20I | TGGCCA | 2 cut(s) | 17, 180 |
| MroI | TCCGGA | 1 cut(s) | 490 |
| MscI | TGGCCA | 2 cut(s) | 17, 180 |
| MseI | TTAA | 2 cut(s) | 297, 984 |
| MslI | CAYNNNNRTG | 2 cut(s) | 723, 762 |
| Msp20I | TGGCCA | 2 cut(s) | 17, 180 |
| MspI | CCGG | 3 cut(s) | 259, 491, 1082 |
| MspR9I | CCNGG | 2 cut(s) | 816, 1082 |
| MunI | CAATTG | 1 cut(s) | 702 |
| MvaI | CCWGG | 1 cut(s) | 816 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 976 |
| NciI | CCSGG | 1 cut(s) | 1082 |
| NcoI | CCATGG | 1 cut(s) | 301 |
| NdeII | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| NlaIII | CATG | 7 cut(s) | 305, 350, 359, 475, 487, 684, 785 |
| NlaIV | GGNNCC | 4 cut(s) | 399, 489, 495, 1080 |
| NmeAIII | GCCGAG | 1 cut(s) | 172 |
| NmuCI | GTSAC | 3 cut(s) | 479, 574, 665 |
| NspI | RCATGY | 1 cut(s) | 475 |
| PaeR7I | CTCGAG | 1 cut(s) | 335 |
| PagI | TCATGA | 1 cut(s) | 355 |
| PceI | AGGCCT | 2 cut(s) | 50, 235 |
| PciSI | GCTCTTC | 1 cut(s) | 21 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 959 |
| PfeI | GAWTC | 2 cut(s) | 59, 96 |
| PkrI | GCNGC | 3 cut(s) | 268, 363, 457 |
| PleI | GAGTC | 1 cut(s) | 603 |
| PpsI | GAGTC | 1 cut(s) | 603 |
| PpuMI | RGGWCCY | 1 cut(s) | 1078 |
| Psp124BI | GAGCTC | 2 cut(s) | 699, 823 |
| Psp5II | RGGWCCY | 1 cut(s) | 1078 |
| Psp6I | CCWGG | 1 cut(s) | 814 |
| PspGI | CCWGG | 1 cut(s) | 814 |
| PspN4I | GGNNCC | 4 cut(s) | 399, 489, 495, 1080 |
| PspPI | GGNCC | 2 cut(s) | 911, 1078 |
| PspPPI | RGGWCCY | 1 cut(s) | 1078 |
| PspXI | VCTCGAGB | 1 cut(s) | 335 |
| PstI | CTGCAG | 1 cut(s) | 271 |
| PsuI | RGATCY | 4 cut(s) | 136, 208, 397, 502 |
| RsaI | GTAC | 4 cut(s) | 386, 440, 561, 974 |
| RsaNI | GTAC | 4 cut(s) | 385, 439, 560, 973 |
| RseI | CAYNNNNRTG | 2 cut(s) | 723, 762 |
| SacI | GAGCTC | 2 cut(s) | 699, 823 |
| SapI | GCTCTTC | 1 cut(s) | 21 |
| SaqAI | TTAA | 2 cut(s) | 297, 984 |
| SatI | GCNGC | 3 cut(s) | 267, 362, 456 |
| Sau3AI | GATC | 7 cut(s) | 136, 208, 246, 397, 502, 754, 883 |
| Sau96I | GGNCC | 2 cut(s) | 911, 1078 |
| ScaI | AGTACT | 1 cut(s) | 386 |
| SchI | GAGTC | 1 cut(s) | 603 |
| ScrFI | CCNGG | 2 cut(s) | 816, 1082 |
| SduI | GDGCHC | 3 cut(s) | 498, 699, 823 |
| SfaNI | GCATC | 2 cut(s) | 321, 661 |
| SfcI | CTRYAG | 1 cut(s) | 267 |
| Sfr274I | CTCGAG | 1 cut(s) | 335 |
| SinI | GGWCC | 2 cut(s) | 911, 1078 |
| SlaI | CTCGAG | 1 cut(s) | 335 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 723, 762 |
| SmlI | CTYRAG | 1 cut(s) | 335 |
| SmoI | CTYRAG | 1 cut(s) | 335 |
| SseBI | AGGCCT | 2 cut(s) | 50, 235 |
| SsiI | CCGC | 1 cut(s) | 262 |
| SspI | AATATT | 1 cut(s) | 1043 |
| SspMI | CTAG | 2 cut(s) | 393, 663 |
| SstI | GAGCTC | 2 cut(s) | 699, 823 |
| StuI | AGGCCT | 2 cut(s) | 50, 235 |
| StyD4I | CCNGG | 2 cut(s) | 814, 1080 |
| StyI | CCWWGG | 2 cut(s) | 301, 365 |
| TaaI | ACNGT | 1 cut(s) | 319 |
| TaqI | TCGA | 4 cut(s) | 87, 336, 965, 1021 |
| TatI | WGTACW | 2 cut(s) | 384, 559 |
| TfiI | GAWTC | 2 cut(s) | 59, 96 |
| Tru1I | TTAA | 2 cut(s) | 297, 984 |
| Tru9I | TTAA | 2 cut(s) | 297, 984 |
| TscAI | CASTG | 2 cut(s) | 324, 797 |
| TseFI | GTSAC | 3 cut(s) | 479, 574, 665 |
| TseI | GCWGC | 3 cut(s) | 266, 361, 455 |
| Tsp45I | GTSAC | 3 cut(s) | 479, 574, 665 |
| TspDTI | ATGAA | 4 cut(s) | 109, 344, 724, 798 |
| TspGWI | ACGGA | 1 cut(s) | 1064 |
| TspRI | CASTG | 2 cut(s) | 324, 797 |
| VpaK11BI | GGWCC | 2 cut(s) | 911, 1078 |
| XapI | RAATTY | 1 cut(s) | 841 |
| XceI | RCATGY | 1 cut(s) | 475 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 319 |
| XhoI | CTCGAG | 1 cut(s) | 335 |
| XmiI | GTMKAC | 1 cut(s) | 196 |
| XspI | CTAG | 2 cut(s) | 393, 663 |
| ZrmI | AGTACT | 1 cut(s) | 386 |