RchiOBHm_Chr4g0391961

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000054
Physical Location & Seq
Forward (+)
7365382 .. 7371563
6182 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36474

Sequence Viewer

Length: 2238 bp
ATGGCAGCTACCAATAAAAATGATGTTTCTACCATCTATGACAGAAAAAGTGAACTGAAAGCTTTTGATGACACAAAGGAAGGTGTTAAAGGCCTTGTTGATGCTGGCATAACCGAGATACCTCGGATCTTTTATCATCCACCTGATGAGTACTCCATTCACAATACCTCTGATTCAGAAGAAGAGCAGTTCAGTGTTCCAGTCATAGACCTCAAGGGCCTTTCAGATCCAACTAAACGAAAGGGGATCGTTGCAGCCGTTGCAGAAGCATCAGAATCTTGGGGCTTTTTTCAAATTGTCAACCATGGAATATCTGTTGATGTGCTTGAGGAGATAAAGGATGGGGTACGAGGGTTTTTTCAGCAGGATACTGCTGTGAAGAAACAGTTTTACACTCGCGATAACTTCAGTAGTCCATTCGTCTACAATAGCAACTTTGATTTGTATAGTGCACCAGCGACTCATTGGAGGGATTCTTTCACGTGGTACATGATCACTCCTACTCCTAAGACAGAGGACCTGCCAGAAGTATGCAGAGAGATACTTGTTGAATACTCAAATCAAGTAAGGAAGTTGGGGAAGCTATTGTTTGAGTTGTTGTCTGAGGCTCTTGGGCTAAAGCAAAGTCACTTGAATGACATAGATTGTAATGAGGGGCTTTTGGTTACAGGCCATTACTATCCTGCTTGTCCACAACCAGAATTAACAGTAGGCATAAGCAAGCATGCAGATAGGACCTTCATCACTGTTCTTCTGCAAGATAATGTTGGTGGTCTTCAAGTCCTTCATCATAACATGTGGATTGATGTAAACCCTGTGCCGGGTGCCCTAGTGCTTATATCAAATGACAGATTCAAGAGTGTAGAACACAGGGTGCTGGCAAACCATAGAGGTCCCAGAATCTCAGTTGCAGGTTTTTTCAGCACAGGATTGCTGCCATTGGGAAAGCTCTACGGACCAATTAAGGAGATATTATCAGAAGACAATCCTCCAAAGTACAGGGAGACCACTTTAAGGGACTATCATGCATACTACAGAGAAAAAGGCCTTGATGGGAGATATTCAATTAAGGATACGAAGTCGCTTTTCGGCATACTCCTTTGTATGGACGCAGAAGTATATCGTTTGACCAAGATGGTGGCTACCAACAGAAATGAGGTTCCAACCAACTATGACAGAAAACATGAACTAAAAGCTTTTGATGATACAAAGGAAGGTGTTAAAGGCCTTGTTGATGCTGGAATAACCGAGATTCCTCGAATTTTTTATCATCCACCAGATGAGTATTCCATCGACAATACCTCTGATTCAGAAGAAGTCCGGTGTAGCATTCCAGTCATAGACGTCGAAGGCCTCTTCGATCAAACAAAACGAAAGGAGATTGTTGCAGCAGTTGGAGAAGCATCAGAGACTTGGGGCTTCTTTCAAATTTCCAATCATGGAATACCTTTTGATGTGCTGGAGGAGATGAAGGATGGGGTACGTGGGTTTTATGAGCAGGATACTGAAGTGAAGAAACAGTATTATACTCGTGATGACTCCAGTAGTACAGTAGTCTACAACAGCAACTTTGATCTGTATAGTGCACCGGCAACTAATTGGAGGGATAGTTTACTGTGTTATATGGCTCCTACTCCTCCTAAGACGGAAGACTTTCCTGAAGTATGCAGAGAAATTCTTGTTGAATACTCGAAGCAAGTAATGAAGTTGGGGAAGCTATTGTTTGAGTTGTTATCCGAGGCTCTTGGGCTAAATCCAAGTCACTTGAATGACATAGATTGCAGTGAGGGGCTTATAGTTTTAGGCCATTATTATCCTGCTTGTCCGCAACCAGAGTTAACAATAGGCACAAGCAAGCATGCAGATAATAGCTTCATGACTGTCCTTCTGCAGGATCATATTGGTGGGCTTCAAGTCCTTCATCAGAACAAATGGATTGATGTACCCCATGTACCGGGTGCTTTAGTCGTTAACATCGGAGATTTTCTTATATCAAATGACAGATTCAAGAGTGTTGAGCACAGGGTACTGGCAAACCATAGAGGTCCCAGGGTATCAGTTGCAAGCTTTTTTAGCACAGGATTGCTGCCTTCGACAAAGCTCTACGGACCCATCAAGGAGTTATTATCAGAAGACAATCCTCCAAAGTATAGGGAGACCACTGTATGGGACTATAATGCCTACTATGTTAAAAAAGGCCTCGATGGGAAATCTGCCTTGTCTCATTTTAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

745

Amino Acids

84.16

Weight (kDa)

5.38

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 66 - 167 3.5e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 225 - 308 1.8e-23 2OG-Fe(II) oxygenase superfamily
DIOX_N PF14226 444 - 543 1.2e-18 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 601 - 692 3.5e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03400 AT1G03400 AT1G03410 AT1G03410 AT1G04350 AT1G06620 AT1G06620 AT1G06640 AT1G06640 AT1G06640 AT1G06645 AT1G06650 AT1G06650 AT2G25450 AT2G30830 AT2G30840 AT2G30840 AT2G30840 AT3G61400 AT3G61400 AT5G43440 AT5G43440 AT5G43450 AT5G43450 AT5G59530 AT5G59540 AT5G59540
fragaria_vesca FvH4_2g03190 FvH4_2g03220 FvH4_3g07861 FvH4_3g31880 FvH4_3g31880 FvH4_3g31880 FvH4_4g03241 FvH4_4g03250 FvH4_4g03251 FvH4_4g03260 FvH4_4g03300 FvH4_4g03301 FvH4_4g03301 FvH4_4g03301 FvH4_4g03302 FvH4_4g03303 FvH4_4g03303 FvH4_4g03304 FvH4_4g03305 FvH4_4g03323 FvH4_4g03323 FvH4_5g19290 FvH4_5g19290 FvH4_5g19290 FvH4_5g19291 FvH4_5g19291 FvH4_5g19310 FvH4_5g19310 FvH4_5g19310 FvH4_5g19320 FvH4_5g19321 FvH4_5g19321 FvH4_5g19321 FvH4_7g31470
malus_domestica MD00G1220300.v1.1 MD02G1050800.v1.1 MD02G1053200.v1.1 MD02G1053300.v1.1 MD02G1054900.v1.1 MD03G1032300.v1.1 MD03G1245000.v1.1 MD03G1245300.v1.1 MD05G1014200.v1.1 MD13G1210900.v1.1 MD13G1211000.v1.1 MD13G1211100.v1.1 MD13G1211200.v1.1 MD13G1211800.v1.1 MD13G1211900.v1.1 MD13G1212000.v1.1 MD16G1214100.v1.1 MD16G1214300.v1.1 MD16G1214400.v1.1 MD16G1214600.v1.1 MD16G1215000.v1.1 MD16G1215200.v1.1 MD16G1215300.v1.1 MD16G1215500.v1.1 MD16G1271000.v1.1
prunus_persica Prupe.1G035500_v2.0.a1 Prupe.1G035700_v2.0.a1 Prupe.1G035800_v2.0.a1 Prupe.1G035900_v2.0.a1 Prupe.1G036000_v2.0.a1 Prupe.1G036100_v2.0.a1 Prupe.1G036200_v2.0.a1 Prupe.1G036400_v2.0.a1 Prupe.1G036500_v2.0.a1 Prupe.1G036600_v2.0.a1 Prupe.1G036700_v2.0.a1 Prupe.1G036800_v2.0.a1 Prupe.1G036800_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G191000_v2.0.a1 Prupe.1G191100_v2.0.a1
pyrus_communis pycom13g18340 pycom13g18350 pycom13g18380 pycom16g17990 pycom16g18010 pycom16g18020
rosa_chinensis RchiOBHm_Chr2g0124051 RchiOBHm_Chr4g0391811 RchiOBHm_Chr4g0391931 RchiOBHm_Chr4g0391961 RchiOBHm_Chr4g0391971 RchiOBHm_Chr4g0391981 RchiOBHm_Chr4g0391991 RchiOBHm_Chr4g0392031 RchiOBHm_Chr4g0392051 RchiOBHm_Chr4g0392061 RchiOBHm_Chr4g0392071 RchiOBHm_Chr4g0392081 RchiOBHm_Chr4g0392091 RchiOBHm_Chr4g0392111 RchiOBHm_Chr4g0392121 RchiOBHm_Chr4g0392131 RchiOBHm_Chr4g0392141 RchiOBHm_Chr4g0392151 RchiOBHm_Chr4g0392211 RchiOBHm_Chr4g0392251 RchiOBHm_Chr4g0392261 RchiOBHm_Chr4g0392301 RchiOBHm_Chr4g0392311 RchiOBHm_Chr4g0392341 RchiOBHm_Chr4g0409221 RchiOBHm_Chr4g0410991 RchiOBHm_Chr4g0429131 RchiOBHm_Chr4g0437051 RchiOBHm_Chr5g0038681 RchiOBHm_Chr5g0047071 RchiOBHm_Chr5g0058651 RchiOBHm_Chr6g0248121 RchiOBHm_Chr6g0248141 RchiOBHm_Chr6g0248291 RchiOBHm_Chr6g0248341 RchiOBHm_Chr6g0248351
rosa_laevigata RLG00000006473 RLG00000006475 RLG00000006667 RLG00000007095 RLG00000009819 RLG00000009820 RLG00000009821 RLG00000009822 RLG00000009825 RLG00000009826 RLG00000009830 RLG00000009831 RLG00000009832 RLG00000009833 RLG00000009834 RLG00000009835 RLG00000009837 RLG00000009838 RLG00000009839 RLG00000009841 RLG00000009842 RLG00000009844 RLG00000009845 RLG00000009846 RLG00000009847 RLG00000009848 RLG00000009849 RLG00000015160 RLG00000015161 RLG00000017791 RLG00000018779 RLG00000034461 RLG00000035237
rosa_multiflora Rmu_co8253791.1_g000001 Rmu_co8348827.1_g000001 Rmu_co8400489.1_g000001 Rmu_sc0000383.1_g000003 Rmu_sc0000383.1_g000005 Rmu_sc0000383.1_g000026 Rmu_sc0000846.1_g000012 Rmu_sc0001231.1_g000013 Rmu_sc0001231.1_g000026 Rmu_sc0001231.1_g000028 Rmu_sc0001800.1_g000027 Rmu_sc0003436.1_g000022 Rmu_sc0004104.1_g000004 Rmu_sc0004295.1_g000021 Rmu_sc0004295.1_g000031 Rmu_sc0004928.1_g000002 Rmu_sc0004928.1_g000008 Rmu_sc0004928.1_g000011 Rmu_sc0004928.1_g000027 Rmu_sc0004977.1_g000007 Rmu_sc0005018.1_g000002 Rmu_sc0005091.1_g000026 Rmu_sc0005091.1_g000032 Rmu_sc0005091.1_g000033 Rmu_sc0005817.1_g000003 Rmu_sc0005817.1_g000008 Rmu_sc0012482.1_g000002 Rmu_sc0014210.1_g000001 Rmu_sc0016150.1_g000001 Rmu_sc0029701.1_g000002 Rmu_sc0036585.1_g000001 Rmu_sc0037489.1_g000002
rosa_roxburghii Rroxscaffold_1G00021790 Rroxscaffold_1G00021800 Rroxscaffold_1G00034360 Rroxscaffold_2G00120130 Rroxscaffold_5G00337210 Rroxscaffold_5G00337220 Rroxscaffold_5G00337230 Rroxscaffold_5G00337250 Rroxscaffold_5G00337260 Rroxscaffold_5G00337270 Rroxscaffold_5G00337300 Rroxscaffold_5G00337310 Rroxscaffold_5G00337320 Rroxscaffold_5G00337330 Rroxscaffold_5G00337350 Rroxscaffold_5G00337370 Rroxscaffold_5G00337390 Rroxscaffold_5G00337400 Rroxscaffold_5G00337420 Rroxscaffold_5G00337430 Rroxscaffold_5G00337450 Rroxscaffold_5G00337470 Rroxscaffold_5G00337480 Rroxscaffold_5G00337520 Rroxscaffold_5G00378130 Rroxscaffold_6G00397800 Rroxscaffold_7G00213320 Rroxscaffold_7G00213390 Rroxscaffold_7G00213420 Rroxscaffold_7G00213470
rosa_rugosa Rorug02G0070100 Rorug02G0070100 Rorug02G0070100 Rorug02G0128600 Rorug02G0128700 Rorug02G0128800 Rorug02G0128900 Rorug02G0248400 Rorug03G0337000 Rorug03G0337000 Rorug03G0337000 Rorug03G0337000 Rorug03G0337100 Rorug03G0337200 Rorug03G0337300 Rorug03G0337600 Rorug03G0337700 Rorug03G0337800 Rorug03G0337900 Rorug03G0337900 Rorug03G0338000 Rorug03G0338100 Rorug03G0338200 Rorug04G0233200.1 Rorug04G0297900 Rorug05G0233700 Rorug05G0316700 Rorug05G0530800 Rorug05G0530900
rosa_samantha Rh2AG307400 Rh2BG316500 Rh2DG331200 Rh4AG039300 Rh4AG039400 Rh4AG039600 Rh4AG039700 Rh4AG039900 Rh4AG040000 Rh4AG040100 Rh4AG040300 Rh4AG040500 Rh4AG040700 Rh4AG041000 Rh4AG041100 Rh4AG041600 Rh4AG041800 Rh4AG041900 Rh4AG352400 Rh4BG033200 Rh4BG034100 Rh4BG034300 Rh4BG034700 Rh4BG034800 Rh4BG035200 Rh4BG035400 Rh4BG036000 Rh4BG036200 Rh4BG036300 Rh4BG036500 Rh4BG036800 Rh4BG361700 Rh4CG042000 Rh4CG042200 Rh4CG042300 Rh4CG042500 Rh4CG042600 Rh4CG042800 Rh4CG043200 Rh4CG043400 Rh4CG043500 Rh4CG043700 Rh4CG043800 Rh4CG044200 Rh4CG044500 Rh4CG044700 Rh4CG177000 Rh4CG310600 Rh4CG376000 Rh4DG036300 Rh4DG036900 Rh4DG037000 Rh4DG037100 Rh4DG037200 Rh4DG037300 Rh4DG037500 Rh4DG037700 Rh4DG037800 Rh4DG037900 Rh4DG038000 Rh4DG038200 Rh4DG038500 Rh4DG038700 Rh4DG038900 Rh4DG159600 Rh5CG347900 Rh5DG409900 Rh6AG045300 Rh6AG045800 Rh6AG046000 Rh6AG046100 Rh6BG040600 Rh6BG040700 Rh6BG042200 Rh6BG042700 Rh6BG042800 Rh6DG035900 Rh6DG036100 Rh6DG036200
rosa_wichuraiana Rw0G010420 Rw2G024780 Rw4G003020 Rw4G003070 Rw4G003080 Rw4G003090 Rw4G003100 Rw4G003130 Rw4G003140 Rw4G003150 Rw4G003180 Rw4G003190 Rw4G003200 Rw4G003210 Rw4G003220 Rw4G003240 Rw4G003270 Rw4G025050 Rw4G030850 Rw6G003930 Rw6G004000 Rw6G004030 Rw6G004040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1347
Acc36I ACCTGC 2 cut(s) 528, 902
AccB1I GGYRCC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 2166
AccI GTMKAC 2 cut(s) 423, 1557
AccII CGCG 1 cut(s) 399
AciI CCGC 1 cut(s) 1826
AclWI GGATC 4 cut(s) 134, 221, 254, 1902
AcsI RAATTY 3 cut(s) 1260, 1428, 1674
AcuI CTGAAG 3 cut(s) 391, 1527, 1680
AcvI CACGTG 1 cut(s) 483
AcyI GRCGYC 1 cut(s) 1344
AdeI CACNNNGTG 1 cut(s) 874
AfaI GTAC 9 cut(s) 152, 348, 488, 998, 1482, 1549, 1944, 1953, 2028
AfiI CCNNNNNNNGG 7 cut(s) 820, 821, 1014, 1105, 1891, 1954, 2166
AflIII ACRYGT 1 cut(s) 795
AjnI CCWGG 1 cut(s) 2048
Alw21I GWGCWC 3 cut(s) 454, 1588, 2022
Alw26I GTCTC 4 cut(s) 998, 1403, 2150, 2226
Alw44I GTGCAC 2 cut(s) 450, 1584
AlwI GGATC 4 cut(s) 134, 221, 254, 1902
AoxI GGCC 8 cut(s) 91, 217, 670, 1045, 1225, 1351, 1804, 2197
ApaLI GTGCAC 2 cut(s) 450, 1584
ApeKI GCWGC 5 cut(s) 5, 254, 934, 1388, 2086
ApoI RAATTY 3 cut(s) 1260, 1428, 1674
Asp700I GAANNNNTTC 1 cut(s) 1653
AspS9I GGNCC 7 cut(s) 217, 517, 735, 893, 956, 2045, 2108
AsuC2I CCSGG 2 cut(s) 822, 1956
AvaII GGWCC 6 cut(s) 517, 735, 893, 956, 2045, 2108
BaeGI GKGCMC 3 cut(s) 454, 829, 1588
BaeI ACNNNNGTAYC 8 cut(s) 338, 360, 371, 393, 1472, 1494, 1505, 1527
BanI GGYRCC 1 cut(s) 824
BauI CACGAG 1 cut(s) 1530
BbrPI CACGTG 1 cut(s) 483
BbsI GAAGAC 4 cut(s) 767, 987, 1656, 2139
Bbv12I GWGCWC 3 cut(s) 454, 1588, 2022
BbvI GCAGC 5 cut(s) 17, 266, 921, 1400, 2073
BccI CCATC 8 cut(s) 41, 335, 1046, 1129, 1298, 1469, 2120, 2198
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 1 cut(s) 2050
BciVI GTATCC 3 cut(s) 361, 1066, 1495
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 2 cut(s) 822, 1956
BcoDI GTCTC 4 cut(s) 998, 1403, 2150, 2226
BfaI CTAG 1 cut(s) 830
BfmI CTRYAG 2 cut(s) 1033, 1889
BfuAI ACCTGC 2 cut(s) 528, 902
BfuI GTATCC 3 cut(s) 361, 1066, 1495
BisI GCNGC 5 cut(s) 6, 255, 935, 1389, 2087
BlsI GCNGC 5 cut(s) 7, 256, 936, 1390, 2088
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 822, 1956, 2050
Bme18I GGWCC 6 cut(s) 517, 735, 893, 956, 2045, 2108
BmgT120I GGNCC 7 cut(s) 217, 517, 735, 893, 956, 2045, 2108
BmiI GGNNCC 6 cut(s) 826, 895, 1161, 1629, 2047, 2110
BmrFI CCNGG 3 cut(s) 822, 1956, 2050
BmsI GCATC 4 cut(s) 91, 278, 1225, 1412
BpiI GAAGAC 4 cut(s) 767, 987, 1656, 2139
BpmI CTGGAG 2 cut(s) 1481, 1525
BpuEI CTTGAG 2 cut(s) 197, 347
BpuMI CCSGG 2 cut(s) 822, 1956
BsaAI YACGTR 2 cut(s) 483, 1484
BsaHI GRCGYC 1 cut(s) 1344
BsaI GGTCTC 2 cut(s) 998, 2150
BsaJI CCNNGG 5 cut(s) 122, 304, 1737, 2048, 2049
BsaWI WCCGGW 1 cut(s) 1320
BsaXI ACNNNNNCTCC 4 cut(s) 487, 517, 1594, 1624
Bsc4I CCNNNNNNNGG 7 cut(s) 820, 821, 1014, 1105, 1891, 1954, 2166
Bse118I RCCGGY 1 cut(s) 1588
Bse1I ACTGG 4 cut(s) 200, 1334, 1542, 2034
BseBI CCWGG 1 cut(s) 2050
BseDI CCNNGG 5 cut(s) 122, 304, 1737, 2048, 2049
BseGI GGATG 4 cut(s) 136, 346, 1270, 1480
BseLI CCNNNNNNNGG 7 cut(s) 820, 821, 1014, 1105, 1891, 1954, 2166
BseMII CTCAG 2 cut(s) 594, 918
BseNI ACTGG 4 cut(s) 200, 1334, 1542, 2034
BseRI GAGGAG 3 cut(s) 344, 1478, 1626
BseSI GKGCMC 3 cut(s) 454, 829, 1588
BseXI GCAGC 5 cut(s) 17, 266, 921, 1400, 2073
Bsh1236I CGCG 1 cut(s) 399
BshFI GGCC 8 cut(s) 93, 219, 672, 1047, 1227, 1353, 1806, 2199
BshNI GGYRCC 1 cut(s) 824
BsiHKAI GWGCWC 3 cut(s) 454, 1588, 2022
BsiSI CCGG 4 cut(s) 821, 1321, 1589, 1955
BslFI GGGAC 4 cut(s) 879, 1031, 2031, 2183
BslI CCNNNNNNNGG 7 cut(s) 820, 821, 1014, 1105, 1891, 1954, 2166
BsmAI GTCTC 4 cut(s) 998, 1403, 2150, 2226
BsmFI GGGAC 4 cut(s) 879, 1031, 2031, 2183
BsmI GAATGC 1 cut(s) 1329
BsnI GGCC 8 cut(s) 93, 219, 672, 1047, 1227, 1353, 1806, 2199
Bso31I GGTCTC 2 cut(s) 998, 2150
Bsp1286I GDGCHC 4 cut(s) 454, 829, 1588, 2022
Bsp143I GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
Bsp19I CCATGG 1 cut(s) 304
Bsp68I TCGCGA 1 cut(s) 399
BspACI CCGC 1 cut(s) 1826
BspANI GGCC 8 cut(s) 93, 219, 672, 1047, 1227, 1353, 1806, 2199
BspCNI CTCAG 2 cut(s) 595, 917
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 1875
BspLI GGNNCC 6 cut(s) 826, 895, 1161, 1629, 2047, 2110
BspMAI CTGCAG 1 cut(s) 1893
BspMI ACCTGC 2 cut(s) 528, 902
BspPI GGATC 4 cut(s) 134, 221, 254, 1902
BspQI GCTCTTC 1 cut(s) 177
BspT107I GGYRCC 1 cut(s) 824
BspTNI GGTCTC 2 cut(s) 998, 2150
BsrFI RCCGGY 1 cut(s) 1588
BsrI ACTGG 4 cut(s) 200, 1334, 1542, 2034
BssAI RCCGGY 1 cut(s) 1588
BssECI CCNNGG 5 cut(s) 122, 304, 1737, 2048, 2049
BssMI GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
BssNI GRCGYC 1 cut(s) 1344
BssSI CACGAG 1 cut(s) 1530
BssT1I CCWWGG 1 cut(s) 304
Bst2BI CACGAG 1 cut(s) 1530
Bst2UI CCWGG 1 cut(s) 2050
Bst4CI ACNGT 8 cut(s) 387, 709, 748, 1521, 1552, 1617, 1882, 2164
Bst6I CTCTTC 2 cut(s) 177, 1361
BstACI GRCGYC 1 cut(s) 1344
BstAPI GCANNNNNTGC 1 cut(s) 260
BstBAI YACGTR 2 cut(s) 483, 1484
BstC8I GCNNGC 7 cut(s) 106, 722, 726, 879, 1856, 1860, 2065
BstDEI CTNAG 4 cut(s) 507, 603, 904, 1641
BstDSI CCRYGG 1 cut(s) 304
BstENI CCTNNNNNAGG 1 cut(s) 1889
BstF5I GGATG 4 cut(s) 136, 346, 1270, 1480
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 7 cut(s) 129, 229, 249, 495, 1363, 1576, 1897
BstMAI GTCTC 4 cut(s) 998, 1403, 2150, 2226
BstMBI GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
BstMWI GCNNNNNNNGC 2 cut(s) 260, 2073
BstNI CCWGG 1 cut(s) 2050
BstNSI RCATGY 3 cut(s) 728, 799, 1862
BstSCI CCNGG 3 cut(s) 820, 1954, 2048
BstSFI CTRYAG 2 cut(s) 1033, 1889
BstSLI GKGCMC 3 cut(s) 454, 829, 1588
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 5 cut(s) 17, 266, 921, 1400, 2073
BstV2I GAAGAC 4 cut(s) 767, 987, 1656, 2139
BstX2I RGATCY 2 cut(s) 126, 226
BstXI CCANNNNNNTGG 1 cut(s) 1138
BstYI RGATCY 2 cut(s) 126, 226
BsuI GTATCC 3 cut(s) 361, 1066, 1495
BsuRI GGCC 8 cut(s) 93, 219, 672, 1047, 1227, 1353, 1806, 2199
BtgI CCRYGG 1 cut(s) 304
BtsCI GGATG 4 cut(s) 136, 346, 1270, 1480
BtsI GCAGTG 1 cut(s) 1789
BtsIMutI CAGTG 4 cut(s) 199, 744, 1789, 2160
BtuMI TCGCGA 1 cut(s) 399
BveI ACCTGC 2 cut(s) 528, 902
Cac8I GCNNGC 7 cut(s) 106, 722, 726, 879, 1856, 1860, 2065
CciI TCATGA 1 cut(s) 1875
Cfr10I RCCGGY 1 cut(s) 1588
Cfr13I GGNCC 7 cut(s) 217, 517, 735, 893, 956, 2045, 2108
CseI GACGC 1 cut(s) 1118
Csp6I GTAC 9 cut(s) 151, 347, 487, 997, 1481, 1548, 1943, 1952, 2027
CviQI GTAC 9 cut(s) 151, 347, 487, 997, 1481, 1548, 1943, 1952, 2027
DdeI CTNAG 4 cut(s) 507, 603, 904, 1641
DpnI GATC 7 cut(s) 128, 228, 248, 494, 1362, 1575, 1896
DpnII GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
DraIII CACNNNGTG 1 cut(s) 874
Eam1104I CTCTTC 2 cut(s) 177, 1361
EarI CTCTTC 2 cut(s) 177, 1361
Eco130I CCWWGG 1 cut(s) 304
Eco147I AGGCCT 5 cut(s) 93, 1047, 1227, 1353, 2199
Eco31I GGTCTC 2 cut(s) 998, 2150
Eco47I GGWCC 6 cut(s) 517, 735, 893, 956, 2045, 2108
Eco57I CTGAAG 3 cut(s) 391, 1527, 1680
Eco72I CACGTG 1 cut(s) 483
EcoNI CCTNNNNNAGG 1 cut(s) 1889
EcoO109I RGGNCCY 5 cut(s) 217, 517, 735, 893, 2045
EcoRII CCWGG 1 cut(s) 2048
EcoT14I CCWWGG 1 cut(s) 304
EcoT22I ATGCAT 1 cut(s) 1030
ErhI CCWWGG 1 cut(s) 304
FaqI GGGAC 4 cut(s) 879, 1031, 2031, 2183
FbaI TGATCA 1 cut(s) 492
FblI GTMKAC 2 cut(s) 423, 1557
Fnu4HI GCNGC 5 cut(s) 6, 255, 935, 1389, 2087
FokI GGATG 4 cut(s) 123, 353, 1257, 1487
Fsp4HI GCNGC 5 cut(s) 6, 255, 935, 1389, 2087
FspBI CTAG 1 cut(s) 830
GluI GCNGC 5 cut(s) 6, 255, 935, 1389, 2087
GsuI CTGGAG 2 cut(s) 1481, 1525
HaeIII GGCC 8 cut(s) 93, 219, 672, 1047, 1227, 1353, 1806, 2199
HapII CCGG 4 cut(s) 821, 1321, 1589, 1955
HgaI GACGC 1 cut(s) 1118
Hin1I GRCGYC 1 cut(s) 1344
HincII GTYRAC 3 cut(s) 301, 1839, 1972
HindII GTYRAC 3 cut(s) 301, 1839, 1972
HindIII AAGCTT 4 cut(s) 60, 1194, 2065, 2231
HpaI GTTAAC 2 cut(s) 1839, 1972
HpaII CCGG 4 cut(s) 821, 1321, 1589, 1955
Hpy188III TCNNGA 6 cut(s) 398, 856, 1532, 1658, 1876, 2008
Hpy99I CGWCG 1 cut(s) 1349
HpyAV CCTTC 9 cut(s) 74, 748, 794, 1208, 1343, 1465, 1895, 1928, 2100
HpyCH4III ACNGT 8 cut(s) 387, 709, 748, 1521, 1552, 1617, 1882, 2164
HpyCH4IV ACGT 3 cut(s) 482, 1344, 1483
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 2073
HpyF3I CTNAG 4 cut(s) 507, 603, 904, 1641
HpySE526I ACGT 3 cut(s) 482, 1344, 1483
Hsp92I GRCGYC 1 cut(s) 1344
Ksp22I TGATCA 1 cut(s) 492
KspAI GTTAAC 2 cut(s) 1839, 1972
Kzo9I GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 1633
Lsp1109I GCAGC 5 cut(s) 17, 266, 921, 1400, 2073
LweI GCATC 4 cut(s) 91, 278, 1225, 1412
MaeI CTAG 1 cut(s) 830
MaeII ACGT 3 cut(s) 482, 1344, 1483
MaeIII GTNAC 3 cut(s) 626, 664, 1760
MalI GATC 7 cut(s) 128, 228, 248, 494, 1362, 1575, 1896
MboI GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
MflI RGATCY 2 cut(s) 126, 226
MhlI GDGCHC 4 cut(s) 454, 829, 1588, 2022
MluCI AATT 8 cut(s) 294, 701, 960, 1065, 1260, 1428, 1597, 1674
MlyI GAGTC 2 cut(s) 454, 1532
MmeI TCCRAC 3 cut(s) 254, 1187, 1375
Mph1103I ATGCAT 1 cut(s) 1030
MroXI GAANNNNTTC 1 cut(s) 1653
MslI CAYNNNNRTG 3 cut(s) 633, 1767, 1902
MspI CCGG 4 cut(s) 821, 1321, 1589, 1955
MspR9I CCNGG 3 cut(s) 822, 1956, 2050
Mva1269I GAATGC 1 cut(s) 1329
MvaI CCWGG 1 cut(s) 2050
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 2 cut(s) 260, 2073
NciI CCSGG 2 cut(s) 822, 1956
NcoI CCATGG 1 cut(s) 304
NdeII GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
NlaIV GGNNCC 6 cut(s) 826, 895, 1161, 1629, 2047, 2110
NmuCI GTSAC 2 cut(s) 626, 1760
NruI TCGCGA 1 cut(s) 399
NsiI ATGCAT 1 cut(s) 1030
NspI RCATGY 3 cut(s) 728, 799, 1862
PaeI GCATGC 2 cut(s) 728, 1862
PagI TCATGA 1 cut(s) 1875
PasI CCCWGGG 1 cut(s) 2049
PceI AGGCCT 5 cut(s) 93, 1047, 1227, 1353, 2199
PciI ACATGT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 177
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PctI GAATGC 1 cut(s) 1329
PdmI GAANNNNTTC 1 cut(s) 1653
PfeI GAWTC 8 cut(s) 173, 275, 473, 852, 900, 1252, 1307, 2004
PflMI CCANNNNNTGG 1 cut(s) 2166
PkrI GCNGC 5 cut(s) 7, 256, 936, 1390, 2088
PleI GAGTC 2 cut(s) 454, 1532
PmaCI CACGTG 1 cut(s) 483
PmlI CACGTG 1 cut(s) 483
PpsI GAGTC 2 cut(s) 454, 1532
Ppu21I YACGTR 2 cut(s) 483, 1484
PpuMI RGGWCCY 4 cut(s) 517, 735, 893, 2045
PscI ACATGT 1 cut(s) 795
Psp5II RGGWCCY 4 cut(s) 517, 735, 893, 2045
Psp6I CCWGG 1 cut(s) 2048
PspCI CACGTG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 2048
PspN4I GGNNCC 6 cut(s) 826, 895, 1161, 1629, 2047, 2110
PspPI GGNCC 7 cut(s) 217, 517, 735, 893, 956, 2045, 2108
PspPPI RGGWCCY 4 cut(s) 517, 735, 893, 2045
PstI CTGCAG 1 cut(s) 1893
PsuI RGATCY 2 cut(s) 126, 226
RruI TCGCGA 1 cut(s) 399
RsaI GTAC 9 cut(s) 152, 348, 488, 998, 1482, 1549, 1944, 1953, 2028
RsaNI GTAC 9 cut(s) 151, 347, 487, 997, 1481, 1548, 1943, 1952, 2027
RseI CAYNNNNRTG 3 cut(s) 633, 1767, 1902
SapI GCTCTTC 1 cut(s) 177
SatI GCNGC 5 cut(s) 6, 255, 935, 1389, 2087
Sau3AI GATC 7 cut(s) 126, 226, 246, 492, 1360, 1573, 1894
Sau96I GGNCC 7 cut(s) 217, 517, 735, 893, 956, 2045, 2108
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 2 cut(s) 454, 1532
ScrFI CCNGG 3 cut(s) 822, 1956, 2050
SduI GDGCHC 4 cut(s) 454, 829, 1588, 2022
SfaNI GCATC 4 cut(s) 91, 278, 1225, 1412
SfcI CTRYAG 2 cut(s) 1033, 1889
SinI GGWCC 6 cut(s) 517, 735, 893, 956, 2045, 2108
SmiMI CAYNNNNRTG 3 cut(s) 633, 1767, 1902
SmlI CTYRAG 2 cut(s) 212, 326
SmoI CTYRAG 2 cut(s) 212, 326
SphI GCATGC 2 cut(s) 728, 1862
Sse9I AATT 8 cut(s) 294, 701, 960, 1065, 1260, 1428, 1597, 1674
SseBI AGGCCT 5 cut(s) 93, 1047, 1227, 1353, 2199
SsiI CCGC 1 cut(s) 1826
SspMI CTAG 1 cut(s) 830
StuI AGGCCT 5 cut(s) 93, 1047, 1227, 1353, 2199
StyD4I CCNGG 3 cut(s) 820, 1954, 2048
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 8 cut(s) 387, 709, 748, 1521, 1552, 1617, 1882, 2164
TaiI ACGT 3 cut(s) 485, 1347, 1486
TaqI TCGA 7 cut(s) 1258, 1293, 1347, 1359, 1691, 2093, 2202
TasI AATT 8 cut(s) 294, 701, 960, 1065, 1260, 1428, 1597, 1674
TatI WGTACW 3 cut(s) 150, 996, 1547
TfiI GAWTC 8 cut(s) 173, 275, 473, 852, 900, 1252, 1307, 2004
TscAI CASTG 4 cut(s) 199, 751, 1789, 2167
TseFI GTSAC 2 cut(s) 626, 1760
TseI GCWGC 5 cut(s) 5, 254, 934, 1388, 2086
Tsp45I GTSAC 2 cut(s) 626, 1760
TspDTI ATGAA 7 cut(s) 730, 776, 1200, 1484, 1718, 1864, 1910
TspGWI ACGGA 3 cut(s) 969, 1661, 2121
TspRI CASTG 4 cut(s) 199, 751, 1789, 2167
Van91I CCANNNNNTGG 1 cut(s) 2166
VneI GTGCAC 2 cut(s) 450, 1584
VpaK11BI GGWCC 6 cut(s) 517, 735, 893, 956, 2045, 2108
XagI CCTNNNNNAGG 1 cut(s) 1889
XapI RAATTY 3 cut(s) 1260, 1428, 1674
XceI RCATGY 3 cut(s) 728, 799, 1862
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XmiI GTMKAC 2 cut(s) 423, 1557
XmnI GAANNNNTTC 1 cut(s) 1653
XspI CTAG 1 cut(s) 830
ZraI GACGTC 1 cut(s) 1345
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.