RchiOBHm_Chr4g0392121

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000054
Physical Location & Seq
Forward (+)
7527318 .. 7528379
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36490

Sequence Viewer

Length: 555 bp
ATGGAGGCCACACGTGACCGCACAAGTGAACTGAAAGCATTTGATGATACAAAAGCCGGTGTGAATGGACTACTCGATGCAGGAGTCACAACAATCCCGCGCATTTTCCACGCATTTCCCACTCTAGATCACAACCAAGTGTTGATCAGAGGGAGGGAAGTGATGATGATGCCCAGCTCAGTATTCCAATTTCCAATCATTGATCTTCCAGGCATAACCCATGGAAACTCAGCTTTACGAGATGAGGTCATTGAGAAAGTTCGACATGCGTGTCAGAAATTGGGTTTCTTTCAGGTAGTGAATCATGGAATTCCGGTAAATGTTTTGGATGAGGTGATTCGAGGTATACGCGAGTTCCATGAGCAGGATAGTGAGCTCAAGAAAGAGTTCTATACAAGAATTTCAGGGAAGAAAGTGTATTATCAGTCTAATTATGATTTGTACCAAGCTTCATCAACTAACTGGAGAGACACACTTGGCTGTTTTATGGTGCCTAATCCACCCAAACCAGAAGAATTACCCACAATATGCAGAGATATAGTGATCGAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

184

Amino Acids

21.04

Weight (kDa)

5.86

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 65 - 164 2.1e-18 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03400 AT1G03400 AT1G03410 AT1G03410 AT1G04350 AT1G06620 AT1G06620 AT1G06640 AT1G06640 AT1G06640 AT1G06645 AT1G06650 AT1G06650 AT2G25450 AT2G30830 AT2G30840 AT2G30840 AT2G30840 AT3G61400 AT3G61400 AT5G43440 AT5G43440 AT5G43450 AT5G43450 AT5G59530 AT5G59540 AT5G59540
fragaria_vesca FvH4_2g03190 FvH4_2g03220 FvH4_3g07861 FvH4_3g31880 FvH4_3g31880 FvH4_3g31880 FvH4_4g03241 FvH4_4g03250 FvH4_4g03251 FvH4_4g03260 FvH4_4g03300 FvH4_4g03301 FvH4_4g03301 FvH4_4g03301 FvH4_4g03302 FvH4_4g03303 FvH4_4g03303 FvH4_4g03304 FvH4_4g03305 FvH4_4g03323 FvH4_4g03323 FvH4_5g19290 FvH4_5g19290 FvH4_5g19290 FvH4_5g19291 FvH4_5g19291 FvH4_5g19310 FvH4_5g19310 FvH4_5g19310 FvH4_5g19320 FvH4_5g19321 FvH4_5g19321 FvH4_5g19321 FvH4_7g31470
malus_domestica MD00G1220300.v1.1 MD02G1050800.v1.1 MD02G1053200.v1.1 MD02G1053300.v1.1 MD02G1054900.v1.1 MD03G1032300.v1.1 MD03G1245000.v1.1 MD03G1245300.v1.1 MD05G1014200.v1.1 MD13G1210900.v1.1 MD13G1211000.v1.1 MD13G1211100.v1.1 MD13G1211200.v1.1 MD13G1211800.v1.1 MD13G1211900.v1.1 MD13G1212000.v1.1 MD16G1214100.v1.1 MD16G1214300.v1.1 MD16G1214400.v1.1 MD16G1214600.v1.1 MD16G1215000.v1.1 MD16G1215200.v1.1 MD16G1215300.v1.1 MD16G1215500.v1.1 MD16G1271000.v1.1
prunus_persica Prupe.1G035500_v2.0.a1 Prupe.1G035700_v2.0.a1 Prupe.1G035800_v2.0.a1 Prupe.1G035900_v2.0.a1 Prupe.1G036000_v2.0.a1 Prupe.1G036100_v2.0.a1 Prupe.1G036200_v2.0.a1 Prupe.1G036400_v2.0.a1 Prupe.1G036500_v2.0.a1 Prupe.1G036600_v2.0.a1 Prupe.1G036700_v2.0.a1 Prupe.1G036800_v2.0.a1 Prupe.1G036800_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G191000_v2.0.a1 Prupe.1G191100_v2.0.a1
pyrus_communis pycom13g18340 pycom13g18350 pycom13g18380 pycom16g17990 pycom16g18010 pycom16g18020
rosa_chinensis RchiOBHm_Chr2g0124051 RchiOBHm_Chr4g0391811 RchiOBHm_Chr4g0391931 RchiOBHm_Chr4g0391961 RchiOBHm_Chr4g0391971 RchiOBHm_Chr4g0391981 RchiOBHm_Chr4g0391991 RchiOBHm_Chr4g0392031 RchiOBHm_Chr4g0392051 RchiOBHm_Chr4g0392061 RchiOBHm_Chr4g0392071 RchiOBHm_Chr4g0392081 RchiOBHm_Chr4g0392091 RchiOBHm_Chr4g0392111 RchiOBHm_Chr4g0392121 RchiOBHm_Chr4g0392131 RchiOBHm_Chr4g0392141 RchiOBHm_Chr4g0392151 RchiOBHm_Chr4g0392211 RchiOBHm_Chr4g0392251 RchiOBHm_Chr4g0392261 RchiOBHm_Chr4g0392301 RchiOBHm_Chr4g0392311 RchiOBHm_Chr4g0392341 RchiOBHm_Chr4g0409221 RchiOBHm_Chr4g0410991 RchiOBHm_Chr4g0429131 RchiOBHm_Chr4g0437051 RchiOBHm_Chr5g0038681 RchiOBHm_Chr5g0047071 RchiOBHm_Chr5g0058651 RchiOBHm_Chr6g0248121 RchiOBHm_Chr6g0248141 RchiOBHm_Chr6g0248291 RchiOBHm_Chr6g0248341 RchiOBHm_Chr6g0248351
rosa_laevigata RLG00000006473 RLG00000006475 RLG00000006667 RLG00000007095 RLG00000009819 RLG00000009820 RLG00000009821 RLG00000009822 RLG00000009825 RLG00000009826 RLG00000009830 RLG00000009831 RLG00000009832 RLG00000009833 RLG00000009834 RLG00000009835 RLG00000009837 RLG00000009838 RLG00000009839 RLG00000009841 RLG00000009842 RLG00000009844 RLG00000009845 RLG00000009846 RLG00000009847 RLG00000009848 RLG00000009849 RLG00000015160 RLG00000015161 RLG00000017791 RLG00000018779 RLG00000034461 RLG00000035237
rosa_multiflora Rmu_co8253791.1_g000001 Rmu_co8348827.1_g000001 Rmu_co8400489.1_g000001 Rmu_sc0000383.1_g000003 Rmu_sc0000383.1_g000005 Rmu_sc0000383.1_g000026 Rmu_sc0000846.1_g000012 Rmu_sc0001231.1_g000013 Rmu_sc0001231.1_g000026 Rmu_sc0001231.1_g000028 Rmu_sc0001800.1_g000027 Rmu_sc0003436.1_g000022 Rmu_sc0004104.1_g000004 Rmu_sc0004295.1_g000021 Rmu_sc0004295.1_g000031 Rmu_sc0004928.1_g000002 Rmu_sc0004928.1_g000008 Rmu_sc0004928.1_g000011 Rmu_sc0004928.1_g000027 Rmu_sc0004977.1_g000007 Rmu_sc0005018.1_g000002 Rmu_sc0005091.1_g000026 Rmu_sc0005091.1_g000032 Rmu_sc0005091.1_g000033 Rmu_sc0005817.1_g000003 Rmu_sc0005817.1_g000008 Rmu_sc0012482.1_g000002 Rmu_sc0014210.1_g000001 Rmu_sc0016150.1_g000001 Rmu_sc0029701.1_g000002 Rmu_sc0036585.1_g000001 Rmu_sc0037489.1_g000002
rosa_roxburghii Rroxscaffold_1G00021790 Rroxscaffold_1G00021800 Rroxscaffold_1G00034360 Rroxscaffold_2G00120130 Rroxscaffold_5G00337210 Rroxscaffold_5G00337220 Rroxscaffold_5G00337230 Rroxscaffold_5G00337250 Rroxscaffold_5G00337260 Rroxscaffold_5G00337270 Rroxscaffold_5G00337300 Rroxscaffold_5G00337310 Rroxscaffold_5G00337320 Rroxscaffold_5G00337330 Rroxscaffold_5G00337350 Rroxscaffold_5G00337370 Rroxscaffold_5G00337390 Rroxscaffold_5G00337400 Rroxscaffold_5G00337420 Rroxscaffold_5G00337430 Rroxscaffold_5G00337450 Rroxscaffold_5G00337470 Rroxscaffold_5G00337480 Rroxscaffold_5G00337520 Rroxscaffold_5G00378130 Rroxscaffold_6G00397800 Rroxscaffold_7G00213320 Rroxscaffold_7G00213390 Rroxscaffold_7G00213420 Rroxscaffold_7G00213470
rosa_rugosa Rorug02G0070100 Rorug02G0070100 Rorug02G0070100 Rorug02G0128600 Rorug02G0128700 Rorug02G0128800 Rorug02G0128900 Rorug02G0248400 Rorug03G0337000 Rorug03G0337000 Rorug03G0337000 Rorug03G0337000 Rorug03G0337100 Rorug03G0337200 Rorug03G0337300 Rorug03G0337600 Rorug03G0337700 Rorug03G0337800 Rorug03G0337900 Rorug03G0337900 Rorug03G0338000 Rorug03G0338100 Rorug03G0338200 Rorug04G0233200.1 Rorug04G0297900 Rorug05G0233700 Rorug05G0316700 Rorug05G0530800 Rorug05G0530900
rosa_samantha Rh2AG307400 Rh2BG316500 Rh2DG331200 Rh4AG039300 Rh4AG039400 Rh4AG039600 Rh4AG039700 Rh4AG039900 Rh4AG040000 Rh4AG040100 Rh4AG040300 Rh4AG040500 Rh4AG040700 Rh4AG041000 Rh4AG041100 Rh4AG041600 Rh4AG041800 Rh4AG041900 Rh4AG352400 Rh4BG033200 Rh4BG034100 Rh4BG034300 Rh4BG034700 Rh4BG034800 Rh4BG035200 Rh4BG035400 Rh4BG036000 Rh4BG036200 Rh4BG036300 Rh4BG036500 Rh4BG036800 Rh4BG361700 Rh4CG042000 Rh4CG042200 Rh4CG042300 Rh4CG042500 Rh4CG042600 Rh4CG042800 Rh4CG043200 Rh4CG043400 Rh4CG043500 Rh4CG043700 Rh4CG043800 Rh4CG044200 Rh4CG044500 Rh4CG044700 Rh4CG177000 Rh4CG310600 Rh4CG376000 Rh4DG036300 Rh4DG036900 Rh4DG037000 Rh4DG037100 Rh4DG037200 Rh4DG037300 Rh4DG037500 Rh4DG037700 Rh4DG037800 Rh4DG037900 Rh4DG038000 Rh4DG038200 Rh4DG038500 Rh4DG038700 Rh4DG038900 Rh4DG159600 Rh5CG347900 Rh5DG409900 Rh6AG045300 Rh6AG045800 Rh6AG046000 Rh6AG046100 Rh6BG040600 Rh6BG040700 Rh6BG042200 Rh6BG042700 Rh6BG042800 Rh6DG035900 Rh6DG036100 Rh6DG036200
rosa_wichuraiana Rw0G010420 Rw2G024780 Rw4G003020 Rw4G003070 Rw4G003080 Rw4G003090 Rw4G003100 Rw4G003130 Rw4G003140 Rw4G003150 Rw4G003180 Rw4G003190 Rw4G003200 Rw4G003210 Rw4G003220 Rw4G003240 Rw4G003270 Rw4G025050 Rw4G030850 Rw6G003930 Rw6G004000 Rw6G004030 Rw6G004040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 346
AccII CGCG 2 cut(s) 100, 351
AciI CCGC 2 cut(s) 19, 98
AcsI RAATTY 2 cut(s) 309, 399
AcvI CACGTG 1 cut(s) 14
AfaI GTAC 2 cut(s) 443, 551
AfiI CCNNNNNNNGG 1 cut(s) 364
AflIII ACRYGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 4 cut(s) 177, 233, 376, 449
AluI AGCT 4 cut(s) 177, 233, 376, 449
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 1 cut(s) 462
AoxI GGCC 1 cut(s) 6
ApoI RAATTY 2 cut(s) 309, 399
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 346
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 378
BbrPI CACGTG 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 378
BciT130I CCWGG 1 cut(s) 210
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 1 cut(s) 125
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 2 cut(s) 67, 159
BpmI CTGGAG 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 313
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 467
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMII CTCAG 2 cut(s) 192, 243
BseNI ACTGG 1 cut(s) 467
BseYI CCCAGC 1 cut(s) 173
Bsh1236I CGCG 2 cut(s) 100, 351
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 378
BsiSI CCGG 2 cut(s) 57, 314
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 1 cut(s) 462
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 4 cut(s) 127, 144, 202, 543
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 2 cut(s) 19, 98
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 191, 242
BspFNI CGCG 2 cut(s) 100, 351
BspLI GGNNCC 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 4 cut(s) 127, 144, 202, 543
BssNAI GTATAC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 220
Bst1107I GTATAC 1 cut(s) 347
Bst2UI CCWGG 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 14
BstDEI CTNAG 2 cut(s) 178, 229
BstDSI CCRYGG 1 cut(s) 220
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 2 cut(s) 100, 351
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 4 cut(s) 130, 147, 205, 546
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 4 cut(s) 127, 144, 202, 543
BstNI CCWGG 1 cut(s) 210
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 208
BstUI CGCG 2 cut(s) 100, 351
BstZ17I GTATAC 1 cut(s) 347
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 334
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 56
Csp6I GTAC 2 cut(s) 442, 550
CviAII CATG 4 cut(s) 221, 266, 305, 359
CviJI RGCY 7 cut(s) 8, 56, 177, 233, 376, 449, 480
CviKI_1 RGCY 7 cut(s) 8, 56, 177, 233, 376, 449, 480
CviQI GTAC 2 cut(s) 442, 550
DdeI CTNAG 2 cut(s) 178, 229
DpnI GATC 4 cut(s) 129, 146, 204, 545
DpnII GATC 4 cut(s) 127, 144, 202, 543
DrdI GACNNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 376
Eco72I CACGTG 1 cut(s) 14
EcoICRI GAGCTC 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 309
EcoRII CCWGG 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 4 cut(s) 224, 269, 308, 362
FatI CATG 4 cut(s) 220, 265, 304, 358
FauI CCCGC 1 cut(s) 105
FbaI TGATCA 1 cut(s) 144
FblI GTMKAC 1 cut(s) 346
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 1 cut(s) 378
FspBI CTAG 1 cut(s) 125
GlaI GCGC 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 1 cut(s) 484
HaeIII GGCC 1 cut(s) 8
HapII CCGG 2 cut(s) 57, 314
HhaI GCGC 1 cut(s) 102
Hin1II CATG 4 cut(s) 224, 269, 308, 362
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 3 cut(s) 84, 301, 337
HpaII CCGG 2 cut(s) 57, 314
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 2 cut(s) 29, 347
Hpy188I TCNGA 2 cut(s) 149, 276
Hpy188III TCNNGA 2 cut(s) 125, 379
Hpy8I GTNNAC 2 cut(s) 29, 347
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 2 cut(s) 80, 531
HpyF3I CTNAG 2 cut(s) 178, 229
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 4 cut(s) 224, 269, 308, 362
HspAI GCGC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 4 cut(s) 127, 144, 202, 543
LweI GCATC 2 cut(s) 67, 159
MaeI CTAG 1 cut(s) 125
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 2 cut(s) 14, 85
MalI GATC 4 cut(s) 129, 146, 204, 545
MboI GATC 4 cut(s) 127, 144, 202, 543
MboII GAAGA 3 cut(s) 197, 421, 524
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 6 cut(s) 188, 278, 309, 399, 430, 515
MlyI GAGTC 1 cut(s) 93
MnlI CCTC 5 cut(s) 143, 147, 238, 325, 335
MroXI GAANNNNTTC 1 cut(s) 386
MspI CCGG 2 cut(s) 57, 314
MspR9I CCNGG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 210
MvnI CGCG 2 cut(s) 100, 351
NcoI CCATGG 1 cut(s) 220
NdeII GATC 4 cut(s) 127, 144, 202, 543
NlaIII CATG 4 cut(s) 224, 269, 308, 362
NlaIV GGNNCC 1 cut(s) 492
NmuCI GTSAC 2 cut(s) 14, 85
NspI RCATGY 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 301, 337
PleI GAGTC 1 cut(s) 92
PmaCI CACGTG 1 cut(s) 14
PmlI CACGTG 1 cut(s) 14
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 208
PspCI CACGTG 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 492
RsaI GTAC 2 cut(s) 443, 551
RsaNI GTAC 2 cut(s) 442, 550
SacI GAGCTC 1 cut(s) 378
Sau3AI GATC 4 cut(s) 127, 144, 202, 543
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 378
SetI ASST 9 cut(s) 16, 179, 235, 249, 297, 336, 346, 378, 451
SfaNI GCATC 2 cut(s) 67, 159
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 188, 278, 309, 399, 430, 515
SsiI CCGC 2 cut(s) 19, 98
SspMI CTAG 1 cut(s) 125
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 220
TaiI ACGT 1 cut(s) 16
TaqI TCGA 4 cut(s) 75, 262, 340, 546
TasI AATT 6 cut(s) 188, 278, 309, 399, 430, 515
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 2 cut(s) 301, 337
TseFI GTSAC 2 cut(s) 14, 85
Tsp45I GTSAC 2 cut(s) 14, 85
TspDTI ATGAA 1 cut(s) 441
XapI RAATTY 2 cut(s) 309, 399
XbaI TCTAGA 1 cut(s) 124
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 1 cut(s) 125
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.