Rw4G025050

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000054
Physical Location & Seq
Reverse (-)
51034755 .. 51036977
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025050.1

Sequence Viewer

Length: 1095 bp
ATGTTGGCCGGGAATTCAAGCGACCAAGAAGACCTTCTTCTCAAACAGGTGAAGGCCTTTTACGAATCGAAAGCTGGTGTGAAAGGGCTCGTCGATGCAGGAATCACGAAAATCCCACAAATTTTCATCCATCCGCCAATAGATCCCGGCCAAGTAACCCAAACCGAGTTCAGTATTCCAGTGGTGGACCTCGCTGACGCTGCGGTTGGCATGAGCAGGCATGCGGAGGTGGCTGAGGGAGTCCGGCGGGCTGCGGAGACTGTGGGCTTGTTCCTGGTGGTGAACCATGGGATACCTAAGAGAGTATTGGAGGAGATGCTGCAGGCGATCCGTGGGTTTCATGAGCTGCCCAAGGAAGTGAAAGCTCACTACTATAGTATAGACCCCAAGAGGAAGGTCAAGTCTGCTGGTAGCCATAAGCTCGGTAAACCAACTTCTCTACCTTGGAGGGACTCTTTGTTATGTGACATGATTTCTGAGCCTCTTGATCCTCAAGAATTGCCTGAGATTTGCAGAGACATAACTATGGAGTACTTAAAACGTGTTCACAAATTAGGTGTCAGTTTATTTGAATTGCTATCTGAGGGACTGGGGCTCAAACCTGACCACCTTCTGGGCTTGGGCTGTGCAAATGGACATTTGATTGCTGGTCATTACTCTCCACCATGCCCTGAGCCTGAATTGACCATCAACAATGGTCAACACACAGATCTCGGTTTTCTCAGCATCGTACTTCAAGACAATATCAATGCACTCCAGCTTTTGTATCAAAACCAGTGGATTGATGTCCCTACCGTGCCCGGAGCTCTCGTGGTGAATATTGGAGATTTCTTGCAGCTACTCTCAAATAATAAATACATAAGTGTCATGCATAGAGTACTTGCCAAGAAGGAAGGTCCAAGAGTTTCTGTGGGATGTTTTTTCAATCACATTCCACAAGAAAATTCTGCAGGGAGACTCTTGGAGCCGATTAAGGAGTTGATATCAGAAAAAAATCCCCCAATATACCGTGGAACAAGTGCGATAGATTATATAGGCCACTACAGGAAAGCGTTGATCAATGGGGTGTGTGGACTCGAATATTTGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

40.16

Weight (kDa)

6.4

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 59 - 157 1e-17 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 214 - 309 4.1e-27 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03400 AT1G03400 AT1G03410 AT1G03410 AT1G04350 AT1G06620 AT1G06620 AT1G06640 AT1G06640 AT1G06640 AT1G06645 AT1G06650 AT1G06650 AT2G25450 AT2G30830 AT2G30840 AT2G30840 AT2G30840 AT3G61400 AT3G61400 AT5G43440 AT5G43440 AT5G43450 AT5G43450 AT5G59530 AT5G59540 AT5G59540
fragaria_vesca FvH4_2g03190 FvH4_2g03220 FvH4_3g07861 FvH4_3g31880 FvH4_3g31880 FvH4_3g31880 FvH4_4g03241 FvH4_4g03250 FvH4_4g03251 FvH4_4g03260 FvH4_4g03300 FvH4_4g03301 FvH4_4g03301 FvH4_4g03301 FvH4_4g03302 FvH4_4g03303 FvH4_4g03303 FvH4_4g03304 FvH4_4g03305 FvH4_4g03323 FvH4_4g03323 FvH4_5g19290 FvH4_5g19290 FvH4_5g19290 FvH4_5g19291 FvH4_5g19291 FvH4_5g19310 FvH4_5g19310 FvH4_5g19310 FvH4_5g19320 FvH4_5g19321 FvH4_5g19321 FvH4_5g19321 FvH4_7g31470
malus_domestica MD00G1220300.v1.1 MD02G1050800.v1.1 MD02G1053200.v1.1 MD02G1053300.v1.1 MD02G1054900.v1.1 MD03G1032300.v1.1 MD03G1245000.v1.1 MD03G1245300.v1.1 MD05G1014200.v1.1 MD13G1210900.v1.1 MD13G1211000.v1.1 MD13G1211100.v1.1 MD13G1211200.v1.1 MD13G1211800.v1.1 MD13G1211900.v1.1 MD13G1212000.v1.1 MD16G1214100.v1.1 MD16G1214300.v1.1 MD16G1214400.v1.1 MD16G1214600.v1.1 MD16G1215000.v1.1 MD16G1215200.v1.1 MD16G1215300.v1.1 MD16G1215500.v1.1 MD16G1271000.v1.1
prunus_persica Prupe.1G035500_v2.0.a1 Prupe.1G035700_v2.0.a1 Prupe.1G035800_v2.0.a1 Prupe.1G035900_v2.0.a1 Prupe.1G036000_v2.0.a1 Prupe.1G036100_v2.0.a1 Prupe.1G036200_v2.0.a1 Prupe.1G036400_v2.0.a1 Prupe.1G036500_v2.0.a1 Prupe.1G036600_v2.0.a1 Prupe.1G036700_v2.0.a1 Prupe.1G036800_v2.0.a1 Prupe.1G036800_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G036900_v2.0.a1 Prupe.1G191000_v2.0.a1 Prupe.1G191100_v2.0.a1
pyrus_communis pycom13g18340 pycom13g18350 pycom13g18380 pycom16g17990 pycom16g18010 pycom16g18020
rosa_chinensis RchiOBHm_Chr2g0124051 RchiOBHm_Chr4g0391811 RchiOBHm_Chr4g0391931 RchiOBHm_Chr4g0391961 RchiOBHm_Chr4g0391971 RchiOBHm_Chr4g0391981 RchiOBHm_Chr4g0391991 RchiOBHm_Chr4g0392031 RchiOBHm_Chr4g0392051 RchiOBHm_Chr4g0392061 RchiOBHm_Chr4g0392071 RchiOBHm_Chr4g0392081 RchiOBHm_Chr4g0392091 RchiOBHm_Chr4g0392111 RchiOBHm_Chr4g0392121 RchiOBHm_Chr4g0392131 RchiOBHm_Chr4g0392141 RchiOBHm_Chr4g0392151 RchiOBHm_Chr4g0392211 RchiOBHm_Chr4g0392251 RchiOBHm_Chr4g0392261 RchiOBHm_Chr4g0392301 RchiOBHm_Chr4g0392311 RchiOBHm_Chr4g0392341 RchiOBHm_Chr4g0409221 RchiOBHm_Chr4g0410991 RchiOBHm_Chr4g0429131 RchiOBHm_Chr4g0437051 RchiOBHm_Chr5g0038681 RchiOBHm_Chr5g0047071 RchiOBHm_Chr5g0058651 RchiOBHm_Chr6g0248121 RchiOBHm_Chr6g0248141 RchiOBHm_Chr6g0248291 RchiOBHm_Chr6g0248341 RchiOBHm_Chr6g0248351
rosa_laevigata RLG00000006473 RLG00000006475 RLG00000006667 RLG00000007095 RLG00000009819 RLG00000009820 RLG00000009821 RLG00000009822 RLG00000009825 RLG00000009826 RLG00000009830 RLG00000009831 RLG00000009832 RLG00000009833 RLG00000009834 RLG00000009835 RLG00000009837 RLG00000009838 RLG00000009839 RLG00000009841 RLG00000009842 RLG00000009844 RLG00000009845 RLG00000009846 RLG00000009847 RLG00000009848 RLG00000009849 RLG00000015160 RLG00000015161 RLG00000017791 RLG00000018779 RLG00000034461 RLG00000035237
rosa_multiflora Rmu_co8253791.1_g000001 Rmu_co8348827.1_g000001 Rmu_co8400489.1_g000001 Rmu_sc0000383.1_g000003 Rmu_sc0000383.1_g000005 Rmu_sc0000383.1_g000026 Rmu_sc0000846.1_g000012 Rmu_sc0001231.1_g000013 Rmu_sc0001231.1_g000026 Rmu_sc0001231.1_g000028 Rmu_sc0001800.1_g000027 Rmu_sc0003436.1_g000022 Rmu_sc0004104.1_g000004 Rmu_sc0004295.1_g000021 Rmu_sc0004295.1_g000031 Rmu_sc0004928.1_g000002 Rmu_sc0004928.1_g000008 Rmu_sc0004928.1_g000011 Rmu_sc0004928.1_g000027 Rmu_sc0004977.1_g000007 Rmu_sc0005018.1_g000002 Rmu_sc0005091.1_g000026 Rmu_sc0005091.1_g000032 Rmu_sc0005091.1_g000033 Rmu_sc0005817.1_g000003 Rmu_sc0005817.1_g000008 Rmu_sc0012482.1_g000002 Rmu_sc0014210.1_g000001 Rmu_sc0016150.1_g000001 Rmu_sc0029701.1_g000002 Rmu_sc0036585.1_g000001 Rmu_sc0037489.1_g000002
rosa_roxburghii Rroxscaffold_1G00021790 Rroxscaffold_1G00021800 Rroxscaffold_1G00034360 Rroxscaffold_2G00120130 Rroxscaffold_5G00337210 Rroxscaffold_5G00337220 Rroxscaffold_5G00337230 Rroxscaffold_5G00337250 Rroxscaffold_5G00337260 Rroxscaffold_5G00337270 Rroxscaffold_5G00337300 Rroxscaffold_5G00337310 Rroxscaffold_5G00337320 Rroxscaffold_5G00337330 Rroxscaffold_5G00337350 Rroxscaffold_5G00337370 Rroxscaffold_5G00337390 Rroxscaffold_5G00337400 Rroxscaffold_5G00337420 Rroxscaffold_5G00337430 Rroxscaffold_5G00337450 Rroxscaffold_5G00337470 Rroxscaffold_5G00337480 Rroxscaffold_5G00337520 Rroxscaffold_5G00378130 Rroxscaffold_6G00397800 Rroxscaffold_7G00213320 Rroxscaffold_7G00213390 Rroxscaffold_7G00213420 Rroxscaffold_7G00213470
rosa_rugosa Rorug02G0070100 Rorug02G0070100 Rorug02G0070100 Rorug02G0128600 Rorug02G0128700 Rorug02G0128800 Rorug02G0128900 Rorug02G0248400 Rorug03G0337000 Rorug03G0337000 Rorug03G0337000 Rorug03G0337000 Rorug03G0337100 Rorug03G0337200 Rorug03G0337300 Rorug03G0337600 Rorug03G0337700 Rorug03G0337800 Rorug03G0337900 Rorug03G0337900 Rorug03G0338000 Rorug03G0338100 Rorug03G0338200 Rorug04G0233200.1 Rorug04G0297900 Rorug05G0233700 Rorug05G0316700 Rorug05G0530800 Rorug05G0530900
rosa_samantha Rh2AG307400 Rh2BG316500 Rh2DG331200 Rh4AG039300 Rh4AG039400 Rh4AG039600 Rh4AG039700 Rh4AG039900 Rh4AG040000 Rh4AG040100 Rh4AG040300 Rh4AG040500 Rh4AG040700 Rh4AG041000 Rh4AG041100 Rh4AG041600 Rh4AG041800 Rh4AG041900 Rh4AG352400 Rh4BG033200 Rh4BG034100 Rh4BG034300 Rh4BG034700 Rh4BG034800 Rh4BG035200 Rh4BG035400 Rh4BG036000 Rh4BG036200 Rh4BG036300 Rh4BG036500 Rh4BG036800 Rh4BG361700 Rh4CG042000 Rh4CG042200 Rh4CG042300 Rh4CG042500 Rh4CG042600 Rh4CG042800 Rh4CG043200 Rh4CG043400 Rh4CG043500 Rh4CG043700 Rh4CG043800 Rh4CG044200 Rh4CG044500 Rh4CG044700 Rh4CG177000 Rh4CG310600 Rh4CG376000 Rh4DG036300 Rh4DG036900 Rh4DG037000 Rh4DG037100 Rh4DG037200 Rh4DG037300 Rh4DG037500 Rh4DG037700 Rh4DG037800 Rh4DG037900 Rh4DG038000 Rh4DG038200 Rh4DG038500 Rh4DG038700 Rh4DG038900 Rh4DG159600 Rh5CG347900 Rh5DG409900 Rh6AG045300 Rh6AG045800 Rh6AG046000 Rh6AG046100 Rh6BG040600 Rh6BG040700 Rh6BG042200 Rh6BG042700 Rh6BG042800 Rh6DG035900 Rh6DG036100 Rh6DG036200
rosa_wichuraiana Rw0G010420 Rw2G024780 Rw4G003020 Rw4G003070 Rw4G003080 Rw4G003090 Rw4G003100 Rw4G003130 Rw4G003140 Rw4G003150 Rw4G003180 Rw4G003190 Rw4G003200 Rw4G003210 Rw4G003220 Rw4G003240 Rw4G003270 Rw4G025050 Rw4G030850 Rw6G003930 Rw6G004000 Rw6G004030 Rw6G004040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 613
AciI CCGC 5 cut(s) 134, 203, 224, 247, 254
AclWI GGATC 3 cut(s) 137, 322, 482
AcoI YGGCCR 2 cut(s) 6, 148
AcsI RAATTY 3 cut(s) 13, 120, 943
AfaI GTAC 3 cut(s) 533, 732, 879
AfiI CCNNNNNNNGG 1 cut(s) 613
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 5 cut(s) 18, 572, 737, 925, 1087
AjnI CCWGG 1 cut(s) 273
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
AluBI AGCT 8 cut(s) 74, 346, 365, 421, 760, 806, 838, 1090
AluI AGCT 8 cut(s) 74, 346, 365, 421, 760, 806, 838, 1090
Alw21I GWGCWC 1 cut(s) 808
Alw26I GTCTC 3 cut(s) 251, 510, 949
AlwI GGATC 3 cut(s) 137, 322, 482
AoxI GGCC 4 cut(s) 6, 54, 148, 1036
ApeKI GCWGC 5 cut(s) 200, 251, 319, 346, 835
ApoI RAATTY 3 cut(s) 13, 120, 943
ArsI GACNNNNNNTTYG 2 cut(s) 543, 575
Asp700I GAANNNNTTC 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 187, 896
AsuC2I CCSGG 3 cut(s) 10, 147, 801
AsuHPI GGTGA 3 cut(s) 61, 292, 826
AvaII GGWCC 2 cut(s) 187, 896
BaeGI GKGCMC 1 cut(s) 801
BanII GRGCYC 3 cut(s) 90, 597, 808
BarI GAAGNNNNNNTAC 4 cut(s) 44, 76, 418, 450
BauI CACGAG 1 cut(s) 809
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 808
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 5 cut(s) 187, 238, 306, 333, 847
BccI CCATC 2 cut(s) 138, 695
BciT130I CCWGG 1 cut(s) 275
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 1 cut(s) 1056
BcnI CCSGG 3 cut(s) 10, 147, 801
BcoDI GTCTC 3 cut(s) 251, 510, 949
BfmI CTRYAG 4 cut(s) 320, 373, 948, 1042
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 709
BisI GCNGC 5 cut(s) 201, 252, 320, 347, 836
BlsI GCNGC 5 cut(s) 202, 253, 321, 348, 837
BmcAI AGTACT 2 cut(s) 533, 879
Bme1390I CCNGG 4 cut(s) 10, 147, 275, 801
Bme18I GGWCC 2 cut(s) 187, 896
BmgT120I GGNCC 2 cut(s) 187, 896
BmiI GGNNCC 1 cut(s) 966
BmrFI CCNGG 4 cut(s) 10, 147, 275, 801
BmrI ACTGGG 1 cut(s) 599
BmsI GCATC 3 cut(s) 85, 306, 735
BmuI ACTGGG 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 740
Bpu10I CCTNAGC 2 cut(s) 234, 672
BpuEI CTTGAG 1 cut(s) 477
BpuMI CCSGG 3 cut(s) 10, 147, 801
BsaJI CCNNGG 5 cut(s) 286, 331, 351, 443, 1009
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 1 cut(s) 613
Bse1I ACTGG 3 cut(s) 179, 594, 775
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 5 cut(s) 286, 331, 351, 443, 1009
BseGI GGATG 3 cut(s) 126, 130, 920
BseLI CCNNNNNNNGG 1 cut(s) 613
BseMII CTCAG 6 cut(s) 225, 468, 495, 573, 663, 736
BseNI ACTGG 3 cut(s) 179, 594, 775
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 5 cut(s) 187, 238, 306, 333, 847
BshFI GGCC 4 cut(s) 8, 56, 150, 1038
BsiHKAI GWGCWC 1 cut(s) 808
BsiSI CCGG 4 cut(s) 9, 147, 244, 801
BslFI GGGAC 3 cut(s) 464, 600, 773
BslI CCNNNNNNNGG 1 cut(s) 613
BsmAI GTCTC 3 cut(s) 251, 510, 949
BsmFI GGGAC 3 cut(s) 464, 600, 773
BsnI GGCC 4 cut(s) 8, 56, 150, 1038
Bsp1286I GDGCHC 4 cut(s) 90, 597, 801, 808
Bsp143I GATC 5 cut(s) 142, 327, 487, 709, 1056
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 5 cut(s) 134, 203, 224, 247, 254
BspANI GGCC 4 cut(s) 8, 56, 150, 1038
BspCNI CTCAG 6 cut(s) 226, 469, 496, 574, 664, 735
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 966
BspMAI CTGCAG 2 cut(s) 324, 952
BspPI GGATC 3 cut(s) 137, 322, 482
BsrI ACTGG 3 cut(s) 179, 594, 775
BssECI CCNNGG 5 cut(s) 286, 331, 351, 443, 1009
BssMI GATC 5 cut(s) 142, 327, 487, 709, 1056
BssSI CACGAG 1 cut(s) 809
BssT1I CCWWGG 3 cut(s) 286, 351, 443
Bst2BI CACGAG 1 cut(s) 809
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 3 cut(s) 262, 796, 1010
BstC8I GCNNGC 4 cut(s) 218, 222, 249, 324
BstDEI CTNAG 7 cut(s) 234, 297, 477, 504, 582, 672, 722
BstDSI CCRYGG 3 cut(s) 286, 331, 1009
BstF5I GGATG 3 cut(s) 126, 130, 920
BstKTI GATC 5 cut(s) 145, 330, 490, 712, 1059
BstMAI GTCTC 3 cut(s) 251, 510, 949
BstMBI GATC 5 cut(s) 142, 327, 487, 709, 1056
BstMWI GCNNNNNNNGC 2 cut(s) 200, 230
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 224
BstSCI CCNGG 4 cut(s) 8, 145, 273, 799
BstSFI CTRYAG 4 cut(s) 320, 373, 948, 1042
BstSLI GKGCMC 1 cut(s) 801
BstV1I GCAGC 5 cut(s) 187, 238, 306, 333, 847
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 2 cut(s) 142, 709
BstYI RGATCY 2 cut(s) 142, 709
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 4 cut(s) 8, 56, 150, 1038
BtgI CCRYGG 3 cut(s) 286, 331, 1009
BtsCI GGATG 3 cut(s) 126, 130, 920
BtsIMutI CAGTG 2 cut(s) 186, 782
Cac8I GCNNGC 4 cut(s) 218, 222, 249, 324
CciI TCATGA 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 187, 896
CseI GACGC 1 cut(s) 206
Csp6I GTAC 3 cut(s) 532, 731, 878
CspCI CAANNNNNGTGG 2 cut(s) 758, 793
CviAII CATG 7 cut(s) 211, 221, 287, 341, 469, 666, 868
CviQI GTAC 3 cut(s) 532, 731, 878
DdeI CTNAG 7 cut(s) 234, 297, 477, 504, 582, 672, 722
DpnI GATC 5 cut(s) 144, 329, 489, 711, 1058
DpnII GATC 5 cut(s) 142, 327, 487, 709, 1056
EaeI YGGCCR 2 cut(s) 6, 148
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 806
Eco130I CCWWGG 3 cut(s) 286, 351, 443
Eco147I AGGCCT 1 cut(s) 56
Eco24I GRGCYC 3 cut(s) 90, 597, 808
Eco32I GATATC 1 cut(s) 984
Eco47I GGWCC 2 cut(s) 187, 896
Eco53kI GAGCTC 1 cut(s) 806
EcoICRI GAGCTC 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 984
EcoT14I CCWWGG 3 cut(s) 286, 351, 443
EcoT22I ATGCAT 1 cut(s) 873
EcoT38I GRGCYC 3 cut(s) 90, 597, 808
ErhI CCWWGG 3 cut(s) 286, 351, 443
FaeI CATG 7 cut(s) 214, 224, 290, 344, 472, 669, 871
FalI AAGNNNNNCTT 4 cut(s) 18, 50, 21, 53
FaqI GGGAC 3 cut(s) 464, 600, 773
FatI CATG 7 cut(s) 210, 220, 286, 340, 468, 665, 867
FauI CCCGC 1 cut(s) 240
FbaI TGATCA 1 cut(s) 1056
Fnu4HI GCNGC 5 cut(s) 201, 252, 320, 347, 836
FokI GGATG 3 cut(s) 113, 117, 927
FriOI GRGCYC 3 cut(s) 90, 597, 808
Fsp4HI GCNGC 5 cut(s) 201, 252, 320, 347, 836
GluI GCNGC 5 cut(s) 201, 252, 320, 347, 836
GsuI CTGGAG 1 cut(s) 740
HaeIII GGCC 4 cut(s) 8, 56, 150, 1038
HapII CCGG 4 cut(s) 9, 147, 244, 801
HgaI GACGC 1 cut(s) 206
Hin1II CATG 7 cut(s) 214, 224, 290, 344, 472, 669, 871
HincII GTYRAC 1 cut(s) 701
HindII GTYRAC 1 cut(s) 701
HinfI GANTC 6 cut(s) 65, 102, 240, 452, 957, 1074
HpaII CCGG 4 cut(s) 9, 147, 244, 801
HphI GGTGA 3 cut(s) 61, 292, 826
Hpy166II GTNNAC 6 cut(s) 187, 283, 428, 547, 701, 1073
Hpy188I TCNGA 3 cut(s) 478, 583, 988
Hpy188III TCNNGA 5 cut(s) 106, 341, 485, 494, 737
Hpy8I GTNNAC 6 cut(s) 187, 283, 428, 547, 701, 1073
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 6 cut(s) 44, 46, 388, 620, 883, 887
HpyCH4III ACNGT 3 cut(s) 262, 796, 1010
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 8 cut(s) 98, 322, 513, 629, 752, 835, 871, 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 230
HpyF3I CTNAG 7 cut(s) 234, 297, 477, 504, 582, 672, 722
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 7 cut(s) 214, 224, 290, 344, 472, 669, 871
Ksp22I TGATCA 1 cut(s) 1056
Kzo9I GATC 5 cut(s) 142, 327, 487, 709, 1056
LmnI GCTCC 2 cut(s) 803, 964
Lsp1109I GCAGC 5 cut(s) 187, 238, 306, 333, 847
LweI GCATC 3 cut(s) 85, 306, 735
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 154, 464
MalI GATC 5 cut(s) 144, 329, 489, 711, 1058
MboI GATC 5 cut(s) 142, 327, 487, 709, 1056
MboII GAAGA 2 cut(s) 29, 41
MflI RGATCY 2 cut(s) 142, 709
MhlI GDGCHC 4 cut(s) 90, 597, 801, 808
MluCI AATT 7 cut(s) 13, 120, 497, 551, 572, 680, 943
MlyI GAGTC 4 cut(s) 249, 446, 951, 1068
MnlI CCTC 9 cut(s) 200, 220, 229, 304, 384, 441, 492, 501, 577
Mph1103I ATGCAT 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 33
MseI TTAA 2 cut(s) 536, 972
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 4 cut(s) 9, 147, 244, 801
MspR9I CCNGG 4 cut(s) 10, 147, 275, 801
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 200, 230
NciI CCSGG 3 cut(s) 10, 147, 801
NcoI CCATGG 1 cut(s) 286
NdeII GATC 5 cut(s) 142, 327, 487, 709, 1056
NlaIII CATG 7 cut(s) 214, 224, 290, 344, 472, 669, 871
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 1 cut(s) 464
NsiI ATGCAT 1 cut(s) 873
NspI RCATGY 1 cut(s) 224
PaeI GCATGC 1 cut(s) 224
PagI TCATGA 1 cut(s) 340
PceI AGGCCT 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 65, 102
PflMI CCANNNNNTGG 1 cut(s) 613
PkrI GCNGC 5 cut(s) 202, 253, 321, 348, 837
PleI GAGTC 4 cut(s) 248, 446, 951, 1068
PpsI GAGTC 4 cut(s) 248, 446, 951, 1068
Psp124BI GAGCTC 1 cut(s) 808
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 966
PspPI GGNCC 2 cut(s) 187, 896
PstI CTGCAG 2 cut(s) 324, 952
PsuI RGATCY 2 cut(s) 142, 709
RsaI GTAC 3 cut(s) 533, 732, 879
RsaNI GTAC 3 cut(s) 532, 731, 878
RseI CAYNNNNRTG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 808
SaqAI TTAA 2 cut(s) 536, 972
SatI GCNGC 5 cut(s) 201, 252, 320, 347, 836
Sau3AI GATC 5 cut(s) 142, 327, 487, 709, 1056
Sau96I GGNCC 2 cut(s) 187, 896
ScaI AGTACT 2 cut(s) 533, 879
SchI GAGTC 4 cut(s) 249, 446, 951, 1068
ScrFI CCNGG 4 cut(s) 10, 147, 275, 801
SduI GDGCHC 4 cut(s) 90, 597, 801, 808
SfaNI GCATC 3 cut(s) 85, 306, 735
SfcI CTRYAG 4 cut(s) 320, 373, 948, 1042
SinI GGWCC 2 cut(s) 187, 896
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 492
SmoI CTYRAG 1 cut(s) 492
SphI GCATGC 1 cut(s) 224
Sse9I AATT 7 cut(s) 13, 120, 497, 551, 572, 680, 943
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 5 cut(s) 134, 203, 224, 247, 254
SspI AATATT 2 cut(s) 820, 1082
SstI GAGCTC 1 cut(s) 808
StuI AGGCCT 1 cut(s) 56
StyD4I CCNGG 4 cut(s) 8, 145, 273, 799
StyI CCWWGG 3 cut(s) 286, 351, 443
TaaI ACNGT 3 cut(s) 262, 796, 1010
TaiI ACGT 1 cut(s) 544
TaqI TCGA 3 cut(s) 68, 93, 1077
TasI AATT 7 cut(s) 13, 120, 497, 551, 572, 680, 943
TatI WGTACW 2 cut(s) 531, 877
TfiI GAWTC 2 cut(s) 65, 102
Tru1I TTAA 2 cut(s) 536, 972
Tru9I TTAA 2 cut(s) 536, 972
TscAI CASTG 2 cut(s) 186, 782
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 5 cut(s) 200, 251, 319, 346, 835
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 2 cut(s) 115, 329
TspGWI ACGGA 1 cut(s) 320
TspRI CASTG 2 cut(s) 186, 782
Van91I CCANNNNNTGG 1 cut(s) 613
VpaK11BI GGWCC 2 cut(s) 187, 896
XapI RAATTY 3 cut(s) 13, 120, 943
XceI RCATGY 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 33
ZrmI AGTACT 2 cut(s) 533, 879
Zsp2I ATGCAT 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.