Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 665 |
| AccB1I | GGYRCC | 3 cut(s) | 472, 644, 665 |
| AccI | GTMKAC | 1 cut(s) | 328 |
| AccII | CGCG | 2 cut(s) | 100, 333 |
| AciI | CCGC | 3 cut(s) | 19, 98, 702 |
| AcoI | YGGCCR | 1 cut(s) | 160 |
| AcsI | RAATTY | 4 cut(s) | 171, 291, 632, 889 |
| AcuI | CTGAAG | 1 cut(s) | 770 |
| AcyI | GRCGYC | 1 cut(s) | 645 |
| AfaI | GTAC | 4 cut(s) | 425, 533, 667, 980 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 346, 378, 752 |
| AflIII | ACRYGT | 1 cut(s) | 247 |
| AgsI | TTSAA | 4 cut(s) | 191, 884, 894, 1024 |
| AjnI | CCWGG | 2 cut(s) | 339, 385 |
| AluBI | AGCT | 8 cut(s) | 215, 358, 713, 758, 790, 816, 870, 1027 |
| AluI | AGCT | 8 cut(s) | 215, 358, 713, 758, 790, 816, 870, 1027 |
| Alw21I | GWGCWC | 2 cut(s) | 360, 760 |
| Alw26I | GTCTC | 2 cut(s) | 444, 771 |
| AoxI | GGCC | 5 cut(s) | 6, 160, 601, 703, 848 |
| ApoI | RAATTY | 4 cut(s) | 171, 291, 632, 889 |
| Asp718I | GGTACC | 1 cut(s) | 665 |
| AspLEI | GCGC | 3 cut(s) | 102, 647, 838 |
| AspS9I | GGNCC | 3 cut(s) | 601, 848, 905 |
| AsuHPI | GGTGA | 2 cut(s) | 328, 667 |
| AvaII | GGWCC | 1 cut(s) | 905 |
| BalI | TGGCCA | 1 cut(s) | 162 |
| BanI | GGYRCC | 3 cut(s) | 472, 644, 665 |
| BanII | GRGCYC | 2 cut(s) | 360, 760 |
| Bbv12I | GWGCWC | 2 cut(s) | 360, 760 |
| BccI | CCATC | 4 cut(s) | 152, 541, 656, 656 |
| BciT130I | CCWGG | 2 cut(s) | 341, 387 |
| BclI | TGATCA | 1 cut(s) | 133 |
| BcoDI | GTCTC | 2 cut(s) | 444, 771 |
| BfaI | CTAG | 2 cut(s) | 114, 761 |
| BfoI | RGCGCY | 1 cut(s) | 648 |
| BisI | GCNGC | 1 cut(s) | 703 |
| BlsI | GCNGC | 1 cut(s) | 704 |
| BmcAI | AGTACT | 1 cut(s) | 533 |
| Bme1390I | CCNGG | 2 cut(s) | 341, 387 |
| Bme18I | GGWCC | 1 cut(s) | 905 |
| BmgT120I | GGNCC | 3 cut(s) | 601, 848, 905 |
| BmiI | GGNNCC | 3 cut(s) | 474, 646, 667 |
| BmrFI | CCNGG | 2 cut(s) | 341, 387 |
| BmsI | GCATC | 2 cut(s) | 67, 440 |
| BpmI | CTGGAG | 2 cut(s) | 369, 466 |
| Bpu10I | CCTNAGC | 1 cut(s) | 624 |
| BpuEI | CTTGAG | 1 cut(s) | 344 |
| BsaHI | GRCGYC | 1 cut(s) | 645 |
| BsaI | GGTCTC | 1 cut(s) | 771 |
| BsaJI | CCNNGG | 2 cut(s) | 202, 386 |
| BsaWI | WCCGGW | 1 cut(s) | 295 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 367, 397, 747, 777 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 346, 378, 752 |
| Bse118I | RCCGGY | 1 cut(s) | 56 |
| Bse1I | ACTGG | 2 cut(s) | 163, 449 |
| BseBI | CCWGG | 2 cut(s) | 341, 387 |
| BseDI | CCNNGG | 2 cut(s) | 202, 386 |
| BseGI | GGATG | 1 cut(s) | 316 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 346, 378, 752 |
| BseMII | CTCAG | 5 cut(s) | 225, 519, 573, 615, 1002 |
| BseNI | ACTGG | 2 cut(s) | 163, 449 |
| Bsh1236I | CGCG | 2 cut(s) | 100, 333 |
| BshFI | GGCC | 5 cut(s) | 8, 162, 603, 705, 850 |
| BshNI | GGYRCC | 3 cut(s) | 472, 644, 665 |
| BsiHKAI | GWGCWC | 2 cut(s) | 360, 760 |
| BsiSI | CCGG | 2 cut(s) | 57, 296 |
| BslFI | GGGAC | 1 cut(s) | 600 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 346, 378, 752 |
| BsmAI | GTCTC | 2 cut(s) | 444, 771 |
| BsmFI | GGGAC | 1 cut(s) | 600 |
| BsnI | GGCC | 5 cut(s) | 8, 162, 603, 705, 850 |
| Bso31I | GGTCTC | 1 cut(s) | 771 |
| Bsp1286I | GDGCHC | 2 cut(s) | 360, 760 |
| Bsp143I | GATC | 4 cut(s) | 116, 133, 184, 525 |
| Bsp19I | CCATGG | 1 cut(s) | 202 |
| BspACI | CCGC | 3 cut(s) | 19, 98, 702 |
| BspANI | GGCC | 5 cut(s) | 8, 162, 603, 705, 850 |
| BspCNI | CTCAG | 5 cut(s) | 224, 518, 574, 616, 1001 |
| BspFNI | CGCG | 2 cut(s) | 100, 333 |
| BspLI | GGNNCC | 3 cut(s) | 474, 646, 667 |
| BspT107I | GGYRCC | 3 cut(s) | 472, 644, 665 |
| BspTNI | GGTCTC | 1 cut(s) | 771 |
| BsrFI | RCCGGY | 1 cut(s) | 56 |
| BsrI | ACTGG | 2 cut(s) | 163, 449 |
| BssAI | RCCGGY | 1 cut(s) | 56 |
| BssECI | CCNNGG | 2 cut(s) | 202, 386 |
| BssMI | GATC | 4 cut(s) | 116, 133, 184, 525 |
| BssNAI | GTATAC | 1 cut(s) | 329 |
| BssNI | GRCGYC | 1 cut(s) | 645 |
| BssT1I | CCWWGG | 1 cut(s) | 202 |
| Bst1107I | GTATAC | 1 cut(s) | 329 |
| Bst2UI | CCWGG | 2 cut(s) | 341, 387 |
| Bst4CI | ACNGT | 1 cut(s) | 579 |
| BstACI | GRCGYC | 1 cut(s) | 645 |
| BstC8I | GCNNGC | 2 cut(s) | 619, 868 |
| BstDEI | CTNAG | 5 cut(s) | 211, 505, 582, 624, 988 |
| BstDSI | CCRYGG | 1 cut(s) | 202 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 376 |
| BstF5I | GGATG | 1 cut(s) | 316 |
| BstFNI | CGCG | 2 cut(s) | 100, 333 |
| BstH2I | RGCGCY | 1 cut(s) | 648 |
| BstHHI | GCGC | 3 cut(s) | 102, 647, 838 |
| BstKTI | GATC | 4 cut(s) | 119, 136, 187, 528 |
| BstMAI | GTCTC | 2 cut(s) | 444, 771 |
| BstMBI | GATC | 4 cut(s) | 116, 133, 184, 525 |
| BstNI | CCWGG | 2 cut(s) | 341, 387 |
| BstNSI | RCATGY | 3 cut(s) | 251, 545, 621 |
| BstSCI | CCNGG | 2 cut(s) | 339, 385 |
| BstUI | CGCG | 2 cut(s) | 100, 333 |
| BstZ17I | GTATAC | 1 cut(s) | 329 |
| BsuRI | GGCC | 5 cut(s) | 8, 162, 603, 705, 850 |
| BtgI | CCRYGG | 1 cut(s) | 202 |
| BtsCI | GGATG | 1 cut(s) | 316 |
| BtsIMutI | CAGTG | 1 cut(s) | 657 |
| Cac8I | GCNNGC | 2 cut(s) | 619, 868 |
| CfoI | GCGC | 3 cut(s) | 102, 647, 838 |
| Cfr10I | RCCGGY | 1 cut(s) | 56 |
| Cfr13I | GGNCC | 3 cut(s) | 601, 848, 905 |
| Csp6I | GTAC | 4 cut(s) | 424, 532, 666, 979 |
| CviAII | CATG | 7 cut(s) | 203, 248, 287, 542, 618, 748, 752 |
| CviQI | GTAC | 4 cut(s) | 424, 532, 666, 979 |
| DdeI | CTNAG | 5 cut(s) | 211, 505, 582, 624, 988 |
| DinI | GGCGCC | 1 cut(s) | 646 |
| DpnI | GATC | 4 cut(s) | 118, 135, 186, 527 |
| DpnII | GATC | 4 cut(s) | 116, 133, 184, 525 |
| EaeI | YGGCCR | 1 cut(s) | 160 |
| Ecl136II | GAGCTC | 2 cut(s) | 358, 758 |
| Eco130I | CCWWGG | 1 cut(s) | 202 |
| Eco24I | GRGCYC | 2 cut(s) | 360, 760 |
| Eco31I | GGTCTC | 1 cut(s) | 771 |
| Eco47I | GGWCC | 1 cut(s) | 905 |
| Eco53kI | GAGCTC | 2 cut(s) | 358, 758 |
| Eco57I | CTGAAG | 1 cut(s) | 770 |
| EcoICRI | GAGCTC | 2 cut(s) | 358, 758 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 376 |
| EcoRI | GAATTC | 4 cut(s) | 171, 291, 632, 889 |
| EcoRII | CCWGG | 2 cut(s) | 339, 385 |
| EcoT14I | CCWWGG | 1 cut(s) | 202 |
| EcoT22I | ATGCAT | 1 cut(s) | 753 |
| EcoT38I | GRGCYC | 2 cut(s) | 360, 760 |
| EgeI | GGCGCC | 1 cut(s) | 646 |
| EheI | GGCGCC | 1 cut(s) | 646 |
| ErhI | CCWWGG | 1 cut(s) | 202 |
| FaeI | CATG | 7 cut(s) | 206, 251, 290, 545, 621, 751, 755 |
| FaqI | GGGAC | 1 cut(s) | 600 |
| FatI | CATG | 7 cut(s) | 202, 247, 286, 541, 617, 747, 751 |
| FauI | CCCGC | 1 cut(s) | 105 |
| FbaI | TGATCA | 1 cut(s) | 133 |
| FblI | GTMKAC | 1 cut(s) | 328 |
| Fnu4HI | GCNGC | 1 cut(s) | 703 |
| FokI | GGATG | 1 cut(s) | 323 |
| FriOI | GRGCYC | 2 cut(s) | 360, 760 |
| Fsp4HI | GCNGC | 1 cut(s) | 703 |
| FspBI | CTAG | 2 cut(s) | 114, 761 |
| GlaI | GCGC | 3 cut(s) | 101, 646, 837 |
| GluI | GCNGC | 1 cut(s) | 703 |
| GsuI | CTGGAG | 2 cut(s) | 369, 466 |
| HaeII | RGCGCY | 1 cut(s) | 648 |
| HaeIII | GGCC | 5 cut(s) | 8, 162, 603, 705, 850 |
| HapII | CCGG | 2 cut(s) | 57, 296 |
| HhaI | GCGC | 3 cut(s) | 102, 647, 838 |
| Hin1I | GRCGYC | 1 cut(s) | 645 |
| Hin1II | CATG | 7 cut(s) | 206, 251, 290, 545, 621, 751, 755 |
| Hin6I | GCGC | 3 cut(s) | 100, 645, 836 |
| HinP1I | GCGC | 3 cut(s) | 100, 645, 836 |
| HindIII | AAGCTT | 2 cut(s) | 711, 868 |
| HinfI | GANTC | 7 cut(s) | 84, 283, 319, 368, 382, 724, 934 |
| HpaII | CCGG | 2 cut(s) | 57, 296 |
| HphI | GGTGA | 2 cut(s) | 328, 667 |
| Hpy166II | GTNNAC | 2 cut(s) | 29, 329 |
| Hpy188I | TCNGA | 6 cut(s) | 138, 170, 583, 878, 905, 991 |
| Hpy188III | TCNNGA | 3 cut(s) | 114, 361, 571 |
| Hpy8I | GTNNAC | 2 cut(s) | 29, 329 |
| HpyAV | CCTTC | 4 cut(s) | 679, 791, 878, 986 |
| HpyCH4III | ACNGT | 1 cut(s) | 579 |
| HpyCH4V | TGCA | 4 cut(s) | 80, 513, 688, 751 |
| HpyF3I | CTNAG | 5 cut(s) | 211, 505, 582, 624, 988 |
| Hsp92I | GRCGYC | 1 cut(s) | 645 |
| Hsp92II | CATG | 7 cut(s) | 206, 251, 290, 545, 621, 751, 755 |
| HspAI | GCGC | 3 cut(s) | 100, 645, 836 |
| KasI | GGCGCC | 1 cut(s) | 644 |
| KpnI | GGTACC | 1 cut(s) | 669 |
| Ksp22I | TGATCA | 1 cut(s) | 133 |
| Kzo9I | GATC | 4 cut(s) | 116, 133, 184, 525 |
| LmnI | GCTCC | 2 cut(s) | 343, 755 |
| LweI | GCATC | 2 cut(s) | 67, 440 |
| MaeI | CTAG | 2 cut(s) | 114, 761 |
| MaeIII | GTNAC | 2 cut(s) | 14, 85 |
| MalI | GATC | 4 cut(s) | 118, 135, 186, 527 |
| MboI | GATC | 4 cut(s) | 116, 133, 184, 525 |
| MboII | GAAGA | 6 cut(s) | 179, 403, 506, 584, 587, 776 |
| MhlI | GDGCHC | 2 cut(s) | 360, 760 |
| MlsI | TGGCCA | 1 cut(s) | 162 |
| MluCI | AATT | 6 cut(s) | 171, 291, 412, 497, 632, 889 |
| MluNI | TGGCCA | 1 cut(s) | 162 |
| Mly113I | GGCGCC | 1 cut(s) | 645 |
| MlyI | GAGTC | 3 cut(s) | 93, 376, 377 |
| MmeI | TCCRAC | 1 cut(s) | 754 |
| MnlI | CCTC | 8 cut(s) | 132, 136, 220, 307, 317, 514, 577, 716 |
| Mox20I | TGGCCA | 1 cut(s) | 162 |
| Mph1103I | ATGCAT | 1 cut(s) | 753 |
| MscI | TGGCCA | 1 cut(s) | 162 |
| MseI | TTAA | 3 cut(s) | 560, 735, 963 |
| MslI | CAYNNNNRTG | 2 cut(s) | 252, 660 |
| Msp20I | TGGCCA | 1 cut(s) | 162 |
| MspI | CCGG | 2 cut(s) | 57, 296 |
| MspR9I | CCNGG | 2 cut(s) | 341, 387 |
| MvaI | CCWGG | 2 cut(s) | 341, 387 |
| MvnI | CGCG | 2 cut(s) | 100, 333 |
| NarI | GGCGCC | 1 cut(s) | 645 |
| NcoI | CCATGG | 1 cut(s) | 202 |
| NdeII | GATC | 4 cut(s) | 116, 133, 184, 525 |
| NlaIII | CATG | 7 cut(s) | 206, 251, 290, 545, 621, 751, 755 |
| NlaIV | GGNNCC | 3 cut(s) | 474, 646, 667 |
| NmuCI | GTSAC | 2 cut(s) | 14, 85 |
| NsiI | ATGCAT | 1 cut(s) | 753 |
| NspI | RCATGY | 3 cut(s) | 251, 545, 621 |
| PaeI | GCATGC | 1 cut(s) | 621 |
| PciI | ACATGT | 1 cut(s) | 247 |
| PfeI | GAWTC | 4 cut(s) | 283, 319, 724, 934 |
| PfoI | TCCNGGA | 1 cut(s) | 339 |
| PkrI | GCNGC | 1 cut(s) | 704 |
| PleI | GAGTC | 3 cut(s) | 92, 376, 376 |
| PluTI | GGCGCC | 1 cut(s) | 648 |
| PpsI | GAGTC | 3 cut(s) | 92, 376, 376 |
| PscI | ACATGT | 1 cut(s) | 247 |
| Psp124BI | GAGCTC | 2 cut(s) | 360, 760 |
| Psp6I | CCWGG | 2 cut(s) | 339, 385 |
| PspGI | CCWGG | 2 cut(s) | 339, 385 |
| PspN4I | GGNNCC | 3 cut(s) | 474, 646, 667 |
| PspPI | GGNCC | 3 cut(s) | 601, 848, 905 |
| RsaI | GTAC | 4 cut(s) | 425, 533, 667, 980 |
| RsaNI | GTAC | 4 cut(s) | 424, 532, 666, 979 |
| RseI | CAYNNNNRTG | 2 cut(s) | 252, 660 |
| SacI | GAGCTC | 2 cut(s) | 360, 760 |
| SaqAI | TTAA | 3 cut(s) | 560, 735, 963 |
| SatI | GCNGC | 1 cut(s) | 703 |
| Sau3AI | GATC | 4 cut(s) | 116, 133, 184, 525 |
| Sau96I | GGNCC | 3 cut(s) | 601, 848, 905 |
| ScaI | AGTACT | 1 cut(s) | 533 |
| SchI | GAGTC | 3 cut(s) | 93, 376, 377 |
| ScrFI | CCNGG | 2 cut(s) | 341, 387 |
| SduI | GDGCHC | 2 cut(s) | 360, 760 |
| SfaNI | GCATC | 2 cut(s) | 67, 440 |
| SfoI | GGCGCC | 1 cut(s) | 646 |
| SinI | GGWCC | 1 cut(s) | 905 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 252, 660 |
| SmlI | CTYRAG | 1 cut(s) | 359 |
| SmoI | CTYRAG | 1 cut(s) | 359 |
| SphI | GCATGC | 1 cut(s) | 621 |
| Sse9I | AATT | 6 cut(s) | 171, 291, 412, 497, 632, 889 |
| SsiI | CCGC | 3 cut(s) | 19, 98, 702 |
| SspDI | GGCGCC | 1 cut(s) | 644 |
| SspMI | CTAG | 2 cut(s) | 114, 761 |
| SstI | GAGCTC | 2 cut(s) | 360, 760 |
| StyD4I | CCNGG | 2 cut(s) | 339, 385 |
| StyI | CCWWGG | 1 cut(s) | 202 |
| TaaI | ACNGT | 1 cut(s) | 579 |
| TaqI | TCGA | 5 cut(s) | 75, 244, 322, 528, 570 |
| TasI | AATT | 6 cut(s) | 171, 291, 412, 497, 632, 889 |
| TatI | WGTACW | 1 cut(s) | 531 |
| TauI | GCSGC | 1 cut(s) | 705 |
| TfiI | GAWTC | 4 cut(s) | 283, 319, 724, 934 |
| Tru1I | TTAA | 3 cut(s) | 560, 735, 963 |
| Tru9I | TTAA | 3 cut(s) | 560, 735, 963 |
| TscAI | CASTG | 1 cut(s) | 664 |
| TseFI | GTSAC | 2 cut(s) | 14, 85 |
| Tsp45I | GTSAC | 2 cut(s) | 14, 85 |
| TspDTI | ATGAA | 1 cut(s) | 661 |
| TspRI | CASTG | 1 cut(s) | 664 |
| VpaK11BI | GGWCC | 1 cut(s) | 905 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 376 |
| XapI | RAATTY | 4 cut(s) | 171, 291, 632, 889 |
| XbaI | TCTAGA | 1 cut(s) | 113 |
| XceI | RCATGY | 3 cut(s) | 251, 545, 621 |
| XmiI | GTMKAC | 1 cut(s) | 328 |
| XspI | CTAG | 2 cut(s) | 114, 761 |
| ZrmI | AGTACT | 1 cut(s) | 533 |
| Zsp2I | ATGCAT | 1 cut(s) | 753 |