Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 213 |
| AccI | GTMKAC | 1 cut(s) | 423 |
| AcsI | RAATTY | 3 cut(s) | 126, 294, 540 |
| AcuI | CTGAAG | 2 cut(s) | 393, 546 |
| AcyI | GRCGYC | 1 cut(s) | 210 |
| AfaI | GTAC | 5 cut(s) | 348, 415, 810, 819, 855 |
| AgsI | TTSAA | 5 cut(s) | 293, 551, 634, 779, 910 |
| AluBI | AGCT | 3 cut(s) | 62, 583, 738 |
| AluI | AGCT | 3 cut(s) | 62, 583, 738 |
| Alw21I | GWGCWC | 1 cut(s) | 454 |
| Alw26I | GTCTC | 1 cut(s) | 269 |
| Alw44I | GTGCAC | 1 cut(s) | 450 |
| AoxI | GGCC | 3 cut(s) | 91, 217, 670 |
| ApaLI | GTGCAC | 1 cut(s) | 450 |
| ApeKI | GCWGC | 1 cut(s) | 254 |
| ApoI | RAATTY | 3 cut(s) | 126, 294, 540 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 519 |
| AsuC2I | CCSGG | 1 cut(s) | 822 |
| BaeGI | GKGCMC | 1 cut(s) | 454 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 338, 360, 371, 393 |
| BauI | CACGAG | 1 cut(s) | 396 |
| BbsI | GAAGAC | 2 cut(s) | 522, 767 |
| Bbv12I | GWGCWC | 1 cut(s) | 454 |
| BbvI | GCAGC | 1 cut(s) | 266 |
| BccI | CCATC | 3 cut(s) | 164, 335, 505 |
| BciVI | GTATCC | 1 cut(s) | 361 |
| BcnI | CCSGG | 1 cut(s) | 822 |
| BcoDI | GTCTC | 1 cut(s) | 269 |
| BfuI | GTATCC | 1 cut(s) | 361 |
| BisI | GCNGC | 1 cut(s) | 255 |
| BlsI | GCNGC | 1 cut(s) | 256 |
| Bme1390I | CCNGG | 1 cut(s) | 822 |
| BmiI | GGNNCC | 2 cut(s) | 27, 495 |
| BmrFI | CCNGG | 1 cut(s) | 822 |
| BmsI | GCATC | 3 cut(s) | 91, 278, 879 |
| BpiI | GAAGAC | 2 cut(s) | 522, 767 |
| BpmI | CTGGAG | 2 cut(s) | 347, 391 |
| BpuMI | CCSGG | 1 cut(s) | 822 |
| BsaAI | YACGTR | 1 cut(s) | 350 |
| BsaHI | GRCGYC | 1 cut(s) | 210 |
| BsaJI | CCNNGG | 1 cut(s) | 603 |
| BsaWI | WCCGGW | 1 cut(s) | 186 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 460, 490, 837, 867 |
| Bse118I | RCCGGY | 1 cut(s) | 454 |
| Bse1I | ACTGG | 2 cut(s) | 200, 408 |
| BseDI | CCNNGG | 1 cut(s) | 603 |
| BseGI | GGATG | 2 cut(s) | 136, 346 |
| BseNI | ACTGG | 2 cut(s) | 200, 408 |
| BseRI | GAGGAG | 2 cut(s) | 344, 492 |
| BseSI | GKGCMC | 1 cut(s) | 454 |
| BseXI | GCAGC | 1 cut(s) | 266 |
| BshFI | GGCC | 3 cut(s) | 93, 219, 672 |
| BsiHKAI | GWGCWC | 1 cut(s) | 454 |
| BsiSI | CCGG | 3 cut(s) | 187, 455, 821 |
| BsmAI | GTCTC | 1 cut(s) | 269 |
| BsmI | GAATGC | 1 cut(s) | 195 |
| BsnI | GGCC | 3 cut(s) | 93, 219, 672 |
| Bsp1286I | GDGCHC | 1 cut(s) | 454 |
| Bsp1407I | TGTACA | 1 cut(s) | 853 |
| Bsp143I | GATC | 3 cut(s) | 226, 439, 760 |
| BspANI | GGCC | 3 cut(s) | 93, 219, 672 |
| BspHI | TCATGA | 1 cut(s) | 741 |
| BspLI | GGNNCC | 2 cut(s) | 27, 495 |
| BsrFI | RCCGGY | 1 cut(s) | 454 |
| BsrGI | TGTACA | 1 cut(s) | 853 |
| BsrI | ACTGG | 2 cut(s) | 200, 408 |
| BssAI | RCCGGY | 1 cut(s) | 454 |
| BssECI | CCNNGG | 1 cut(s) | 603 |
| BssMI | GATC | 3 cut(s) | 226, 439, 760 |
| BssNI | GRCGYC | 1 cut(s) | 210 |
| BssSI | CACGAG | 1 cut(s) | 396 |
| Bst2BI | CACGAG | 1 cut(s) | 396 |
| Bst4CI | ACNGT | 4 cut(s) | 387, 418, 483, 748 |
| Bst6I | CTCTTC | 1 cut(s) | 227 |
| BstACI | GRCGYC | 1 cut(s) | 210 |
| BstAUI | TGTACA | 1 cut(s) | 853 |
| BstBAI | YACGTR | 1 cut(s) | 350 |
| BstC8I | GCNNGC | 2 cut(s) | 722, 726 |
| BstDEI | CTNAG | 1 cut(s) | 507 |
| BstF5I | GGATG | 2 cut(s) | 136, 346 |
| BstKTI | GATC | 3 cut(s) | 229, 442, 763 |
| BstMAI | GTCTC | 1 cut(s) | 269 |
| BstMBI | GATC | 3 cut(s) | 226, 439, 760 |
| BstNSI | RCATGY | 1 cut(s) | 728 |
| BstSCI | CCNGG | 1 cut(s) | 820 |
| BstSLI | GKGCMC | 1 cut(s) | 454 |
| BstV1I | GCAGC | 1 cut(s) | 266 |
| BstV2I | GAAGAC | 2 cut(s) | 522, 767 |
| BsuI | GTATCC | 1 cut(s) | 361 |
| BsuRI | GGCC | 3 cut(s) | 93, 219, 672 |
| BtsCI | GGATG | 2 cut(s) | 136, 346 |
| BtsI | GCAGTG | 1 cut(s) | 655 |
| BtsIMutI | CAGTG | 2 cut(s) | 488, 655 |
| Cac8I | GCNNGC | 2 cut(s) | 722, 726 |
| CciI | TCATGA | 1 cut(s) | 741 |
| Cfr10I | RCCGGY | 1 cut(s) | 454 |
| Csp6I | GTAC | 5 cut(s) | 347, 414, 809, 818, 854 |
| CviAII | CATG | 5 cut(s) | 50, 305, 725, 742, 815 |
| CviQI | GTAC | 5 cut(s) | 347, 414, 809, 818, 854 |
| DdeI | CTNAG | 1 cut(s) | 507 |
| DpnI | GATC | 3 cut(s) | 228, 441, 762 |
| DpnII | GATC | 3 cut(s) | 226, 439, 760 |
| Eam1104I | CTCTTC | 1 cut(s) | 227 |
| EarI | CTCTTC | 1 cut(s) | 227 |
| Eco147I | AGGCCT | 2 cut(s) | 93, 219 |
| Eco57I | CTGAAG | 2 cut(s) | 393, 546 |
| FaeI | CATG | 5 cut(s) | 53, 308, 728, 745, 818 |
| FatI | CATG | 5 cut(s) | 49, 304, 724, 741, 814 |
| FblI | GTMKAC | 1 cut(s) | 423 |
| Fnu4HI | GCNGC | 1 cut(s) | 255 |
| FokI | GGATG | 2 cut(s) | 123, 353 |
| Fsp4HI | GCNGC | 1 cut(s) | 255 |
| GluI | GCNGC | 1 cut(s) | 255 |
| GsuI | CTGGAG | 2 cut(s) | 347, 391 |
| HaeIII | GGCC | 3 cut(s) | 93, 219, 672 |
| HapII | CCGG | 3 cut(s) | 187, 455, 821 |
| Hin1I | GRCGYC | 1 cut(s) | 210 |
| Hin1II | CATG | 5 cut(s) | 53, 308, 728, 745, 818 |
| HincII | GTYRAC | 2 cut(s) | 705, 838 |
| HindII | GTYRAC | 2 cut(s) | 705, 838 |
| HindIII | AAGCTT | 1 cut(s) | 60 |
| HinfI | GANTC | 3 cut(s) | 118, 173, 404 |
| HpaI | GTTAAC | 2 cut(s) | 705, 838 |
| HpaII | CCGG | 3 cut(s) | 187, 455, 821 |
| Hpy166II | GTNNAC | 6 cut(s) | 424, 452, 479, 692, 705, 838 |
| Hpy188I | TCNGA | 6 cut(s) | 172, 178, 274, 604, 792, 845 |
| Hpy188III | TCNNGA | 3 cut(s) | 398, 524, 742 |
| Hpy8I | GTNNAC | 6 cut(s) | 424, 452, 479, 692, 705, 838 |
| Hpy99I | CGWCG | 1 cut(s) | 215 |
| HpyAV | CCTTC | 5 cut(s) | 74, 209, 331, 761, 794 |
| HpyCH4III | ACNGT | 4 cut(s) | 387, 418, 483, 748 |
| HpyCH4IV | ACGT | 2 cut(s) | 210, 349 |
| HpyCH4V | TGCA | 6 cut(s) | 254, 452, 534, 648, 728, 757 |
| HpyF3I | CTNAG | 1 cut(s) | 507 |
| HpySE526I | ACGT | 2 cut(s) | 210, 349 |
| Hsp92I | GRCGYC | 1 cut(s) | 210 |
| Hsp92II | CATG | 5 cut(s) | 53, 308, 728, 745, 818 |
| KspAI | GTTAAC | 2 cut(s) | 705, 838 |
| Kzo9I | GATC | 3 cut(s) | 226, 439, 760 |
| LmnI | GCTCC | 1 cut(s) | 499 |
| Lsp1109I | GCAGC | 1 cut(s) | 266 |
| LweI | GCATC | 3 cut(s) | 91, 278, 879 |
| MaeII | ACGT | 2 cut(s) | 210, 349 |
| MaeIII | GTNAC | 1 cut(s) | 626 |
| MalI | GATC | 3 cut(s) | 228, 441, 762 |
| MboI | GATC | 3 cut(s) | 226, 439, 760 |
| MboII | GAAGA | 5 cut(s) | 191, 214, 391, 527, 767 |
| MhlI | GDGCHC | 1 cut(s) | 454 |
| MluCI | AATT | 4 cut(s) | 126, 294, 463, 540 |
| MlyI | GAGTC | 1 cut(s) | 398 |
| MmeI | TCCRAC | 2 cut(s) | 53, 241 |
| MroXI | GAANNNNTTC | 1 cut(s) | 519 |
| MseI | TTAA | 4 cut(s) | 87, 704, 837, 866 |
| MslI | CAYNNNNRTG | 2 cut(s) | 633, 768 |
| MspI | CCGG | 3 cut(s) | 187, 455, 821 |
| MspR9I | CCNGG | 1 cut(s) | 822 |
| Mva1269I | GAATGC | 1 cut(s) | 195 |
| NciI | CCSGG | 1 cut(s) | 822 |
| NdeII | GATC | 3 cut(s) | 226, 439, 760 |
| NlaIII | CATG | 5 cut(s) | 53, 308, 728, 745, 818 |
| NlaIV | GGNNCC | 2 cut(s) | 27, 495 |
| NmuCI | GTSAC | 1 cut(s) | 626 |
| NspI | RCATGY | 1 cut(s) | 728 |
| PaeI | GCATGC | 1 cut(s) | 728 |
| PagI | TCATGA | 1 cut(s) | 741 |
| PceI | AGGCCT | 2 cut(s) | 93, 219 |
| PctI | GAATGC | 1 cut(s) | 195 |
| PdmI | GAANNNNTTC | 1 cut(s) | 519 |
| PfeI | GAWTC | 2 cut(s) | 118, 173 |
| PkrI | GCNGC | 1 cut(s) | 256 |
| PleI | GAGTC | 1 cut(s) | 398 |
| PpsI | GAGTC | 1 cut(s) | 398 |
| Ppu21I | YACGTR | 1 cut(s) | 350 |
| PspN4I | GGNNCC | 2 cut(s) | 27, 495 |
| RsaI | GTAC | 5 cut(s) | 348, 415, 810, 819, 855 |
| RsaNI | GTAC | 5 cut(s) | 347, 414, 809, 818, 854 |
| RseI | CAYNNNNRTG | 2 cut(s) | 633, 768 |
| SaqAI | TTAA | 4 cut(s) | 87, 704, 837, 866 |
| SatI | GCNGC | 1 cut(s) | 255 |
| Sau3AI | GATC | 3 cut(s) | 226, 439, 760 |
| SchI | GAGTC | 1 cut(s) | 398 |
| ScrFI | CCNGG | 1 cut(s) | 822 |
| SduI | GDGCHC | 1 cut(s) | 454 |
| SfaNI | GCATC | 3 cut(s) | 91, 278, 879 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 633, 768 |
| SphI | GCATGC | 1 cut(s) | 728 |
| Sse9I | AATT | 4 cut(s) | 126, 294, 463, 540 |
| SseBI | AGGCCT | 2 cut(s) | 93, 219 |
| StuI | AGGCCT | 2 cut(s) | 93, 219 |
| StyD4I | CCNGG | 1 cut(s) | 820 |
| TaaI | ACNGT | 4 cut(s) | 387, 418, 483, 748 |
| TaiI | ACGT | 2 cut(s) | 213, 352 |
| TaqI | TCGA | 6 cut(s) | 124, 159, 213, 225, 557, 903 |
| TasI | AATT | 4 cut(s) | 126, 294, 463, 540 |
| TatI | WGTACW | 2 cut(s) | 413, 853 |
| TfiI | GAWTC | 2 cut(s) | 118, 173 |
| Tru1I | TTAA | 4 cut(s) | 87, 704, 837, 866 |
| Tru9I | TTAA | 4 cut(s) | 87, 704, 837, 866 |
| TscAI | CASTG | 2 cut(s) | 488, 655 |
| TseFI | GTSAC | 1 cut(s) | 626 |
| TseI | GCWGC | 1 cut(s) | 254 |
| Tsp45I | GTSAC | 1 cut(s) | 626 |
| TspDTI | ATGAA | 5 cut(s) | 66, 350, 584, 730, 776 |
| TspRI | CASTG | 2 cut(s) | 488, 655 |
| VneI | GTGCAC | 1 cut(s) | 450 |
| XapI | RAATTY | 3 cut(s) | 126, 294, 540 |
| XceI | RCATGY | 1 cut(s) | 728 |
| XmiI | GTMKAC | 1 cut(s) | 423 |
| XmnI | GAANNNNTTC | 1 cut(s) | 519 |
| ZraI | GACGTC | 1 cut(s) | 211 |