Belongs to the iron ascorbate-dependent oxidoreductase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 228 |
| AciI | CCGC | 1 cut(s) | 232 |
| AclWI | GGATC | 4 cut(s) | 323, 402, 415, 678 |
| AcsI | RAATTY | 3 cut(s) | 144, 705, 748 |
| AcuI | CTGAAG | 3 cut(s) | 26, 499, 795 |
| AfaI | GTAC | 5 cut(s) | 27, 275, 351, 383, 835 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 199, 620 |
| AflII | CTTAAG | 1 cut(s) | 212 |
| AflIII | ACRYGT | 1 cut(s) | 391 |
| AluBI | AGCT | 7 cut(s) | 68, 77, 290, 502, 537, 626, 720 |
| AluI | AGCT | 7 cut(s) | 68, 77, 290, 502, 537, 626, 720 |
| Alw21I | GWGCWC | 1 cut(s) | 628 |
| Alw26I | GTCTC | 3 cut(s) | 54, 459, 578 |
| AlwI | GGATC | 4 cut(s) | 323, 402, 415, 678 |
| AoxI | GGCC | 3 cut(s) | 698, 728, 764 |
| ApeKI | GCWGC | 1 cut(s) | 487 |
| ApoI | RAATTY | 3 cut(s) | 144, 705, 748 |
| AspS9I | GGNCC | 2 cut(s) | 698, 764 |
| AsuHPI | GGTGA | 1 cut(s) | 535 |
| BamHI | GGATCC | 1 cut(s) | 407 |
| BanII | GRGCYC | 1 cut(s) | 628 |
| Bbv12I | GWGCWC | 1 cut(s) | 628 |
| BbvI | GCAGC | 1 cut(s) | 474 |
| BccI | CCATC | 3 cut(s) | 391, 419, 524 |
| BciVI | GTATCC | 1 cut(s) | 295 |
| BcoDI | GTCTC | 3 cut(s) | 54, 459, 578 |
| BfaI | CTAG | 1 cut(s) | 629 |
| BfrI | CTTAAG | 1 cut(s) | 212 |
| BfuI | GTATCC | 1 cut(s) | 295 |
| BglII | AGATCT | 1 cut(s) | 857 |
| BisI | GCNGC | 1 cut(s) | 488 |
| BlsI | GCNGC | 1 cut(s) | 489 |
| BmcAI | AGTACT | 1 cut(s) | 383 |
| BmgT120I | GGNCC | 2 cut(s) | 698, 764 |
| BmiI | GGNNCC | 2 cut(s) | 409, 455 |
| BsaAI | YACGTR | 2 cut(s) | 25, 185 |
| BsaI | GGTCTC | 2 cut(s) | 54, 578 |
| BsaJI | CCNNGG | 3 cut(s) | 139, 480, 615 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 615, 645, 826, 856 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 199, 620 |
| BseDI | CCNNGG | 3 cut(s) | 139, 480, 615 |
| BseGI | GGATG | 2 cut(s) | 84, 535 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 199, 620 |
| BseMII | CTCAG | 3 cut(s) | 69, 369, 423 |
| BseXI | GCAGC | 1 cut(s) | 474 |
| BseYI | CCCAGC | 1 cut(s) | 64 |
| BshFI | GGCC | 3 cut(s) | 700, 730, 766 |
| BsiHKAI | GWGCWC | 1 cut(s) | 628 |
| BslFI | GGGAC | 1 cut(s) | 253 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 199, 620 |
| BsmAI | GTCTC | 3 cut(s) | 54, 459, 578 |
| BsmFI | GGGAC | 1 cut(s) | 253 |
| BsnI | GGCC | 3 cut(s) | 700, 730, 766 |
| Bso31I | GGTCTC | 2 cut(s) | 54, 578 |
| Bsp1286I | GDGCHC | 1 cut(s) | 628 |
| Bsp143I | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| Bsp19I | CCATGG | 1 cut(s) | 139 |
| BspACI | CCGC | 1 cut(s) | 232 |
| BspANI | GGCC | 3 cut(s) | 700, 730, 766 |
| BspCNI | CTCAG | 3 cut(s) | 70, 368, 424 |
| BspLI | GGNNCC | 2 cut(s) | 409, 455 |
| BspPI | GGATC | 4 cut(s) | 323, 402, 415, 678 |
| BspTI | CTTAAG | 1 cut(s) | 212 |
| BspTNI | GGTCTC | 2 cut(s) | 54, 578 |
| BssECI | CCNNGG | 3 cut(s) | 139, 480, 615 |
| BssMI | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| BssT1I | CCWWGG | 1 cut(s) | 139 |
| BstAFI | CTTAAG | 1 cut(s) | 212 |
| BstBAI | YACGTR | 2 cut(s) | 25, 185 |
| BstC8I | GCNNGC | 2 cut(s) | 521, 718 |
| BstDEI | CTNAG | 4 cut(s) | 78, 355, 432, 461 |
| BstDSI | CCRYGG | 2 cut(s) | 139, 615 |
| BstF5I | GGATG | 2 cut(s) | 84, 535 |
| BstKTI | GATC | 6 cut(s) | 40, 331, 410, 562, 673, 860 |
| BstMAI | GTCTC | 3 cut(s) | 54, 459, 578 |
| BstMBI | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 74 |
| BstNSI | RCATGY | 2 cut(s) | 108, 395 |
| BstSNI | TACGTA | 1 cut(s) | 25 |
| BstV1I | GCAGC | 1 cut(s) | 474 |
| BstX2I | RGATCY | 2 cut(s) | 407, 857 |
| BstYI | RGATCY | 2 cut(s) | 407, 857 |
| BsuI | GTATCC | 1 cut(s) | 295 |
| BsuRI | GGCC | 3 cut(s) | 700, 730, 766 |
| BtgI | CCRYGG | 2 cut(s) | 139, 615 |
| BtsCI | GGATG | 2 cut(s) | 84, 535 |
| BtsIMutI | CAGTG | 1 cut(s) | 602 |
| Cac8I | GCNNGC | 2 cut(s) | 521, 718 |
| Cfr13I | GGNCC | 2 cut(s) | 698, 764 |
| Csp6I | GTAC | 5 cut(s) | 26, 274, 350, 382, 834 |
| CviAII | CATG | 5 cut(s) | 105, 140, 194, 392, 469 |
| CviQI | GTAC | 5 cut(s) | 26, 274, 350, 382, 834 |
| DdeI | CTNAG | 4 cut(s) | 78, 355, 432, 461 |
| DpnI | GATC | 6 cut(s) | 39, 330, 409, 561, 672, 859 |
| DpnII | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| DraI | TTTAAA | 1 cut(s) | 753 |
| Ecl136II | GAGCTC | 1 cut(s) | 626 |
| Eco105I | TACGTA | 1 cut(s) | 25 |
| Eco130I | CCWWGG | 1 cut(s) | 139 |
| Eco24I | GRGCYC | 1 cut(s) | 628 |
| Eco31I | GGTCTC | 2 cut(s) | 54, 578 |
| Eco53kI | GAGCTC | 1 cut(s) | 626 |
| Eco57I | CTGAAG | 3 cut(s) | 26, 499, 795 |
| EcoICRI | GAGCTC | 1 cut(s) | 626 |
| EcoRI | GAATTC | 1 cut(s) | 144 |
| EcoT14I | CCWWGG | 1 cut(s) | 139 |
| EcoT22I | ATGCAT | 1 cut(s) | 106 |
| EcoT38I | GRGCYC | 1 cut(s) | 628 |
| ErhI | CCWWGG | 1 cut(s) | 139 |
| FaeI | CATG | 5 cut(s) | 108, 143, 197, 395, 472 |
| FaqI | GGGAC | 1 cut(s) | 253 |
| FatI | CATG | 5 cut(s) | 104, 139, 193, 391, 468 |
| FauNDI | CATATG | 1 cut(s) | 733 |
| Fnu4HI | GCNGC | 1 cut(s) | 488 |
| FokI | GGATG | 2 cut(s) | 71, 542 |
| FriOI | GRGCYC | 1 cut(s) | 628 |
| Fsp4HI | GCNGC | 1 cut(s) | 488 |
| FspBI | CTAG | 1 cut(s) | 629 |
| GluI | GCNGC | 1 cut(s) | 488 |
| GsaI | CCCAGC | 1 cut(s) | 68 |
| HaeIII | GGCC | 3 cut(s) | 700, 730, 766 |
| Hin1II | CATG | 5 cut(s) | 108, 143, 197, 395, 472 |
| HincII | GTYRAC | 2 cut(s) | 604, 637 |
| HindII | GTYRAC | 2 cut(s) | 604, 637 |
| HindIII | AAGCTT | 1 cut(s) | 718 |
| HinfI | GANTC | 1 cut(s) | 592 |
| HpaI | GTTAAC | 1 cut(s) | 604 |
| HphI | GGTGA | 1 cut(s) | 535 |
| Hpy166II | GTNNAC | 6 cut(s) | 22, 136, 209, 604, 637, 760 |
| Hpy188I | TCNGA | 3 cut(s) | 433, 453, 775 |
| Hpy188III | TCNNGA | 2 cut(s) | 88, 326 |
| Hpy8I | GTNNAC | 6 cut(s) | 22, 136, 209, 604, 637, 760 |
| HpyAV | CCTTC | 2 cut(s) | 238, 614 |
| HpyCH4IV | ACGT | 3 cut(s) | 24, 184, 606 |
| HpyCH4V | TGCA | 3 cut(s) | 104, 363, 556 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 74 |
| HpyF3I | CTNAG | 4 cut(s) | 78, 355, 432, 461 |
| HpySE526I | ACGT | 3 cut(s) | 24, 184, 606 |
| Hsp92II | CATG | 5 cut(s) | 108, 143, 197, 395, 472 |
| KspAI | GTTAAC | 1 cut(s) | 604 |
| Kzo9I | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| LmnI | GCTCC | 1 cut(s) | 623 |
| LpnPI | CCDG | 9 cut(s) | 29, 78, 87, 101, 113, 162, 185, 354, 712 |
| Lsp1109I | GCAGC | 1 cut(s) | 474 |
| MaeI | CTAG | 1 cut(s) | 629 |
| MaeII | ACGT | 3 cut(s) | 24, 184, 606 |
| MaeIII | GTNAC | 1 cut(s) | 423 |
| MalI | GATC | 6 cut(s) | 39, 330, 409, 561, 672, 859 |
| MboI | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| MboII | GAAGA | 6 cut(s) | 32, 229, 273, 356, 700, 788 |
| MflI | RGATCY | 2 cut(s) | 407, 857 |
| MhlI | GDGCHC | 1 cut(s) | 628 |
| MluCI | AATT | 7 cut(s) | 144, 262, 268, 292, 472, 705, 748 |
| MnlI | CCTC | 6 cut(s) | 291, 364, 427, 475, 621, 650 |
| Mph1103I | ATGCAT | 1 cut(s) | 106 |
| MseI | TTAA | 6 cut(s) | 213, 447, 603, 752, 810, 818 |
| MslI | CAYNNNNRTG | 1 cut(s) | 528 |
| MspA1I | CMGCKG | 1 cut(s) | 77 |
| MspCI | CTTAAG | 1 cut(s) | 212 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 74 |
| NcoI | CCATGG | 1 cut(s) | 139 |
| NdeI | CATATG | 1 cut(s) | 733 |
| NdeII | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| NlaIII | CATG | 5 cut(s) | 108, 143, 197, 395, 472 |
| NlaIV | GGNNCC | 2 cut(s) | 409, 455 |
| NmeAIII | GCCGAG | 1 cut(s) | 505 |
| NsiI | ATGCAT | 1 cut(s) | 106 |
| NspI | RCATGY | 2 cut(s) | 108, 395 |
| PciI | ACATGT | 1 cut(s) | 391 |
| PfeI | GAWTC | 1 cut(s) | 592 |
| PkrI | GCNGC | 1 cut(s) | 489 |
| Ppu21I | YACGTR | 2 cut(s) | 25, 185 |
| PscI | ACATGT | 1 cut(s) | 391 |
| PsiI | TTATAA | 1 cut(s) | 228 |
| Psp124BI | GAGCTC | 1 cut(s) | 628 |
| PspFI | CCCAGC | 1 cut(s) | 64 |
| PspN4I | GGNNCC | 2 cut(s) | 409, 455 |
| PspPI | GGNCC | 2 cut(s) | 698, 764 |
| PsuI | RGATCY | 2 cut(s) | 407, 857 |
| PvuII | CAGCTG | 1 cut(s) | 77 |
| RsaI | GTAC | 5 cut(s) | 27, 275, 351, 383, 835 |
| RsaNI | GTAC | 5 cut(s) | 26, 274, 350, 382, 834 |
| RseI | CAYNNNNRTG | 1 cut(s) | 528 |
| SacI | GAGCTC | 1 cut(s) | 628 |
| SaqAI | TTAA | 6 cut(s) | 213, 447, 603, 752, 810, 818 |
| SatI | GCNGC | 1 cut(s) | 488 |
| Sau3AI | GATC | 6 cut(s) | 37, 328, 407, 559, 670, 857 |
| Sau96I | GGNCC | 2 cut(s) | 698, 764 |
| ScaI | AGTACT | 1 cut(s) | 383 |
| SduI | GDGCHC | 1 cut(s) | 628 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 528 |
| SmlI | CTYRAG | 1 cut(s) | 212 |
| SmoI | CTYRAG | 1 cut(s) | 212 |
| SnaBI | TACGTA | 1 cut(s) | 25 |
| Sse9I | AATT | 7 cut(s) | 144, 262, 268, 292, 472, 705, 748 |
| SsiI | CCGC | 1 cut(s) | 232 |
| SspMI | CTAG | 1 cut(s) | 629 |
| SstI | GAGCTC | 1 cut(s) | 628 |
| StyI | CCWWGG | 1 cut(s) | 139 |
| TaiI | ACGT | 3 cut(s) | 27, 187, 609 |
| TaqI | TCGA | 1 cut(s) | 97 |
| TasI | AATT | 7 cut(s) | 144, 262, 268, 292, 472, 705, 748 |
| TatI | WGTACW | 1 cut(s) | 381 |
| TfiI | GAWTC | 1 cut(s) | 592 |
| Tru1I | TTAA | 6 cut(s) | 213, 447, 603, 752, 810, 818 |
| Tru9I | TTAA | 6 cut(s) | 213, 447, 603, 752, 810, 818 |
| TscAI | CASTG | 1 cut(s) | 602 |
| TseI | GCWGC | 1 cut(s) | 487 |
| TspDTI | ATGAA | 4 cut(s) | 73, 273, 485, 529 |
| TspGWI | ACGGA | 1 cut(s) | 632 |
| TspRI | CASTG | 1 cut(s) | 602 |
| Vha464I | CTTAAG | 1 cut(s) | 212 |
| XapI | RAATTY | 3 cut(s) | 144, 705, 748 |
| XceI | RCATGY | 2 cut(s) | 108, 395 |
| XspI | CTAG | 1 cut(s) | 629 |
| ZrmI | AGTACT | 1 cut(s) | 383 |
| Zsp2I | ATGCAT | 1 cut(s) | 106 |