pycom15g07900

calcium ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000732
Physical Location & Seq
Forward (+)
4881198 .. 4881518
321 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 321 bp
ATGCCCAATCTAATCCCCCCAAAGCAGCGTGAGACTTCACCAAATGAAAAAAACAAGGATACGTATAAGCGGGTCAAAATACCCTACACAAAGGAGCAGATATTGAAGATTTTCAAAAGATACGACAAGGACGGAGACGGCCAACTTTCCAAGGCCGAGCTCAAGGAAGCTTTCGCTGAACTCGGCGCGATATGCCCTGGCCTTAGAGCTTGGGATGGTCGTAGGCATGCTGATGACGACGGCGACGGCTTCATTTCTATAGACAAGGAGTTGGACAAGCTTGGCGACTATACCGTGGGCATTGGGTATATCGTAAATTAG

Protein Analysis

107

Amino Acids

12.03

Weight (kDa)

6.58

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 31 - 60 3.8e-06 EF-hand domain pair
EF-hand_1 PF00036 34 - 60 9.5e-08 EF hand domain
EF-hand_6 PF13405 34 - 62 9.3e-08 EF-hand domain
EF-hand_5 PF13202 37 - 58 6.9e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g34500 FvH4_2g34510 FvH4_2g34530
prunus_persica Prupe.1G436400_v2.0.a1 Prupe.1G436500_v2.0.a1 Prupe.1G436600_v2.0.a1 Prupe.1G436700_v2.0.a1 Prupe.1G436800_v2.0.a1 Prupe.1G436900_v2.0.a1 Prupe.1G437000_v2.0.a1 Prupe.1G437100_v2.0.a1 Prupe.1G437200_v2.0.a1
pyrus_communis pycom08g08330 pycom08g08350 pycom08g08360 pycom08g08370 pycom08g08380 pycom15g07870 pycom15g07900
rosa_chinensis RchiOBHm_Chr3g0490571 RchiOBHm_Chr6g0278301 RchiOBHm_Chr6g0278331 RchiOBHm_Chr6g0307631 RchiOBHm_Chr6g0307841 RchiOBHm_Chr7g0227521
rosa_laevigata RLG00000001641 RLG00000006852 RLG00000010696 RLG00000010698 RLG00000010699 RLG00000010700 RLG00000013236
rosa_multiflora Rmu_co8134650.1_g000001 Rmu_sc0000031.1_g000033 Rmu_sc0000031.1_g000046 Rmu_sc0000791.1_g000066 Rmu_sc0001016.1_g000015 Rmu_sc0001016.1_g000032 Rmu_sc0001772.1_g000026 Rmu_sc0001772.1_g000027 Rmu_sc0001772.1_g000034 Rmu_sc0001772.1_g000067 Rmu_sc0003252.1_g000012 Rmu_sc0003862.1_g000006
rosa_roxburghii Rroxscaffold_1G00006670 Rroxscaffold_3G00232100 Rroxscaffold_5G00373780 Rroxscaffold_6G00393410 Rroxscaffold_7G00160830 Rroxscaffold_7G00160860 Rroxscaffold_7G00160880 Rroxscaffold_7G00190470
rosa_rugosa Rorug03G0243900 Rorug04G0257200 Rorug06G0116100 Rorug06G0361100 Rorug06G0361200 Rorug06G0361200 Rorug06G0361300
rosa_samantha Rh2AG314100 Rh4AG313500 Rh6BG229500 Rh6BG480600 Rh6BG480800 Rh6BG480900 Rh6BG481100 Rh7AG392700 Rh7AG394500 Rh7AG394600 Rh7AG394700 Rh7CG412100
rosa_wichuraiana Rw0G001040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 188
AciI CCGC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 139
AgsI TTSAA 2 cut(s) 106, 115
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 4 cut(s) 160, 170, 209, 280
AluI AGCT 4 cut(s) 160, 170, 209, 280
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 2 cut(s) 26, 129
AoxI GGCC 3 cut(s) 139, 153, 199
ApeKI GCWGC 1 cut(s) 25
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 30
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 37
BccI CCATC 1 cut(s) 209
BceAI ACGGC 3 cut(s) 154, 256, 262
BciT130I CCWGG 1 cut(s) 198
BciVI GTATCC 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 26, 129
BfmI CTRYAG 1 cut(s) 258
BfuI GTATCC 1 cut(s) 52
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 150, 196, 294
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 3 cut(s) 150, 196, 294
BseGI GGATG 1 cut(s) 220
BseXI GCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 3 cut(s) 141, 155, 201
BsiHKAI GWGCWC 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 26, 129
BsmBI CGTCTC 1 cut(s) 129
BsnI GGCC 3 cut(s) 141, 155, 201
Bsp1286I GDGCHC 1 cut(s) 162
BspACI CCGC 1 cut(s) 70
BspANI GGCC 3 cut(s) 141, 155, 201
BspFNI CGCG 1 cut(s) 188
BssECI CCNNGG 3 cut(s) 150, 196, 294
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 203
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 188
BstMAI GTCTC 2 cut(s) 26, 129
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 198
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 1 cut(s) 258
BstSNI TACGTA 1 cut(s) 63
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 37
BsuI GTATCC 1 cut(s) 52
BsuRI GGCC 3 cut(s) 141, 155, 201
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 220
Cac8I GCNNGC 1 cut(s) 228
CfoI GCGC 1 cut(s) 188
CviAII CATG 1 cut(s) 227
CviJI RGCY 8 cut(s) 141, 155, 160, 170, 201, 209, 249, 280
CviKI_1 RGCY 8 cut(s) 141, 155, 160, 170, 201, 209, 249, 280
DdeI CTNAG 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 160
Eco105I TACGTA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 1 cut(s) 230
FaiI YATR 6 cut(s) 66, 193, 228, 260, 291, 309
FatI CATG 1 cut(s) 226
FauI CCCGC 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 26
GlaI GCGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 26
HaeIII GGCC 3 cut(s) 141, 155, 201
HhaI GCGC 1 cut(s) 188
Hin1II CATG 1 cut(s) 230
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HindIII AAGCTT 2 cut(s) 168, 278
HphI GGTGA 1 cut(s) 30
Hpy99I CGWCG 2 cut(s) 242, 248
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 1 cut(s) 230
HspAI GCGC 1 cut(s) 186
LmnI GCTCC 1 cut(s) 94
LpnPI CCDG 2 cut(s) 183, 210
Lsp1109I GCAGC 1 cut(s) 37
MaeII ACGT 1 cut(s) 62
MboII GAAGA 1 cut(s) 118
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 198
MvaI CCWGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 192
NlaIII CATG 1 cut(s) 230
NmeAIII GCCGAG 2 cut(s) 162, 181
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 110
PkrI GCNGC 1 cut(s) 27
Ppu21I YACGTR 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 231
SacI GAGCTC 1 cut(s) 162
SatI GCNGC 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 162
SetI ASST 5 cut(s) 65, 162, 172, 211, 282
SfcI CTRYAG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SnaBI TACGTA 1 cut(s) 63
SphI GCATGC 1 cut(s) 230
Sse9I AATT 1 cut(s) 316
SsiI CCGC 1 cut(s) 70
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 1 cut(s) 65
TasI AATT 1 cut(s) 316
TseI GCWGC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 60, 241
TspGWI ACGGA 1 cut(s) 147
XceI RCATGY 1 cut(s) 230
XmnI GAANNNNTTC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.