pycom17g22110

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000202
Physical Location & Seq
Reverse (-)
20501481 .. 20505219
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22110.6

Sequence Viewer

Length: 2046 bp
ATGGCAGTGGACGTTGCAGAATTTCAATGGAGAAAGTTCTTGAGCTCGCTTCGAGACGCCGAGTCGGAATTATCAGGTTCAATTTCCATCATGTACTTCAGAACAATTAAAAATCTCATCTCTTCCTTCAAGTCGATCAAAAATGGGGCCAGAGAGATAGATATAATGCCCCGCCCTCCTTACGACAACTCTGAAATCATCGATTTTCTCTACAAACTCAACGATGCGTTGGCCGAGTGCCGAATCTTCGCAAGGGAATGCAAGGAGCTCAACAAGAAGCTCTTACTCTTCAACCCTTTTGGATTTTACTTCATCCAGAAGATGAATAATCGACTGGATGGATTGAGGAAGGAGCTGGAGAGTTTGGGGGGCGCCCGGCGGCTGGACAATGACGTCGGAGATTGTTGGGCTGAGGAGGTGGAAATGCCACCTTGTCCATTTGTTGATGGGTTTGATGAGCAGGTTGTTTATGGGTTTGATGAGCAGGCAGAGAAAGTTGAGGACTTGCTGTGTGGGGAGGGCTCTACCGGAAATGGGTTTAGGACAATTGGGGTGTTGGGGATTGCTGGTGTGGGTAAGACCACCCTGGTGCACAAGGTTTTGAAAATGGAGAGAGTTAAGGGTAAGTTTAATCCTATAATTTGGTTACCCTTTTCGGGTATGAGGAAAGAGGAAGAACAGTATAGTAGGTTTAGCTTTGAGACGTGCATTGTCGATAATTTGGGCCAAACTCTGGATGGGAGGATAGTTGGCCTTGGTAAGCTCTTGGAAAGGCTCAACCAACTGTTTTCTGGTAAGAGGTATCTGATTGTGTTGGATGATGTGCAGCAGCGCCACATTAACATTGAGGATCGCTTATGGCGCGGATTGCCTAAGGGTAGCGGTGGTGCGGTCATTGTCACTAGTAGGTTAACAGAAGTGGCTCGAAAGGTGGTGGAAGAAAGGGACTTGATTTATGTTGAGCCTTTGGACAAAGAAATTTGCTGGAGCATATTTGAGGAGACGATGAAGAATAACAAAGAAGTTTTAAACATTTCATGTCACACAACTTTGCGTAAGATTGAGAATGAAATTAAGGATCAATGTCATGGCCTTCCATTGGCTGCTAAGGCGCTTGCAGGAATCATTCCGGAGCAGATTCGTGAGATTGAGTCCAAACGTTTCTTGAAGGAGATGTATATACCTGATGAATTGCTTAAACATGATTTGGTTGGTGAACCTTCTGTCGATATACCAAGCTGCCCAGTTTTAGTGTTTGTTGATATAAAAGATGAAAAGCTTGGAGTACAACTCTATAACGAATTTTGCTACCGTCTTAATAAAAAGCAGGTCTTTGCTGTGCTGGAAGAGGACTCTGATTCTAAGGGACCAATAAAGGTAGAGGATCCTGAGGGCGTGCTAAAGGAGGTTTATGGTGCTCTACATAAGAGTAAAAAATATGAGTTTGCTGATGATATTCAAAAGAGGATGAGAATTATAATTGTTGGTGGGGGTGATGTGGTTAACCGGATTCTTGGAGTTATTTGTGATCTGAAATTACCAGAGTCGCCCTCCATTGTGCCAATATCACATGGGACTGAAAATAACATCCCACTTTCGTTTGGATGGAGGGTGCCTAAAGTTGAGCGTCAATCAGTGACATCATTCCTGTATCAAGTAAAAGAGGCACAACAGATAAAAACTGACAGCTGGCATATTCTCATTAAGATGAGAACTACTCAAAGTTCTACAGATCGTAAAGAAATACCACACTCTTTTCATGTATTTCGTCCTGTTCGCAAAGGGGCCACAGAAACTGAGGTTCACAAAGGGGATACAGAAACTGAGGTTCACAAAGGGGCTACAGAAACTGAGGTTCACAAAGGGGATACAGAAACTGAGATTCACGAAGGGGATACAGAAACAGAGGTTCACAAAGGGGCAACAGAAATTGAGGTTCACAAAGGGGCTACAGAAACTGACGTTCACAAAGGGGCTACAGAAACTAAGGTTCACAAAGGGGCTACAGAAACTGAGATAACACCTTGGCAGTGGAGAAATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

682

Amino Acids

77.43

Weight (kDa)

5.67

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1478
AasI GACNNNNNNGTC 1 cut(s) 392
AatII GACGTC 1 cut(s) 396
Acc36I ACCTGC 2 cut(s) 451, 1318
AccB1I GGYRCC 2 cut(s) 371, 1612
AccB7I CCANNNNNTGG 1 cut(s) 733
AccII CGCG 1 cut(s) 864
AccIII TCCGGA 1 cut(s) 1129
AciI CCGC 5 cut(s) 172, 379, 864, 882, 890
AclI AACGTT 1 cut(s) 1159
AclWI GGATC 4 cut(s) 858, 1086, 1379, 1392
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 3 cut(s) 20, 978, 1301
AcuI CTGAAG 1 cut(s) 82
AcyI GRCGYC 3 cut(s) 57, 372, 393
AfaI GTAC 2 cut(s) 95, 1287
AfiI CCNNNNNNNGG 5 cut(s) 382, 534, 656, 733, 1099
AgsI TTSAA 7 cut(s) 26, 81, 130, 292, 604, 1168, 1460
AhdI GACNNNNNGTC 1 cut(s) 61
AhlI ACTAGT 1 cut(s) 902
AjiI CACGTC 1 cut(s) 705
AjnI CCWGG 1 cut(s) 585
AleI CACNNNNGTG 1 cut(s) 587
AluBI AGCT 9 cut(s) 45, 268, 280, 355, 696, 763, 1239, 1279, 1689
AluI AGCT 9 cut(s) 45, 268, 280, 355, 696, 763, 1239, 1279, 1689
Alw21I GWGCWC 4 cut(s) 47, 270, 594, 1420
Alw26I GTCTC 3 cut(s) 48, 695, 995
Alw44I GTGCAC 1 cut(s) 590
AlwI GGATC 4 cut(s) 858, 1086, 1379, 1392
AlwNI CAGNNNCTG 6 cut(s) 1796, 1823, 1850, 1877, 1958, 2012
Aor13HI TCCGGA 1 cut(s) 1129
AoxI GGCC 6 cut(s) 147, 231, 724, 751, 1090, 1785
ApaLI GTGCAC 1 cut(s) 590
ApeKI GCWGC 4 cut(s) 826, 829, 1103, 1239
ApoI RAATTY 3 cut(s) 20, 978, 1301
AspLEI GCGC 4 cut(s) 374, 834, 864, 1114
AspS9I GGNCC 4 cut(s) 147, 724, 1367, 1785
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 1226, 1505
AvaII GGWCC 1 cut(s) 1367
AxyI CCTNAGG 2 cut(s) 873, 1389
BaeGI GKGCMC 1 cut(s) 594
BamHI GGATCC 1 cut(s) 1384
BanI GGYRCC 2 cut(s) 371, 1612
BanII GRGCYC 3 cut(s) 47, 270, 524
Bbv12I GWGCWC 4 cut(s) 47, 270, 594, 1420
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 4 cut(s) 838, 841, 1090, 1226
BccI CCATC 5 cut(s) 95, 332, 440, 731, 1599
BciT130I CCWGG 1 cut(s) 587
BciVI GTATCC 3 cut(s) 1807, 1861, 1888
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 3 cut(s) 48, 695, 995
BcuI ACTAGT 1 cut(s) 902
BfaI CTAG 1 cut(s) 903
BfmI CTRYAG 5 cut(s) 1728, 1842, 1950, 1977, 2004
BfoI RGCGCY 3 cut(s) 375, 835, 1115
BfuAI ACCTGC 2 cut(s) 451, 1318
BfuI GTATCC 3 cut(s) 1807, 1861, 1888
BisI GCNGC 5 cut(s) 380, 827, 830, 1104, 1240
BlsI GCNGC 5 cut(s) 381, 828, 831, 1105, 1241
Bme1390I CCNGG 2 cut(s) 376, 587
Bme18I GGWCC 1 cut(s) 1367
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgBI CACGTC 1 cut(s) 705
BmgT120I GGNCC 4 cut(s) 147, 724, 1367, 1785
BmiI GGNNCC 6 cut(s) 148, 373, 1368, 1386, 1614, 1786
BmrFI CCNGG 2 cut(s) 376, 587
BmrI ACTGGG 1 cut(s) 1238
BmsI GCATC 1 cut(s) 214
BmuI ACTGGG 1 cut(s) 1238
BplI GAGNNNNNCTC 6 cut(s) 1275, 1307, 1535, 1567, 1702, 1734
BpmI CTGGAG 2 cut(s) 377, 1006
Bpu10I CCTNAGC 2 cut(s) 411, 1107
BpuEI CTTGAG 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 376
Bsa29I ATCGAT 1 cut(s) 201
BsaHI GRCGYC 3 cut(s) 57, 372, 393
BsaJI CCNNGG 3 cut(s) 585, 754, 2024
BsaWI WCCGGW 3 cut(s) 527, 1129, 1506
Bsc4I CCNNNNNNNGG 5 cut(s) 382, 534, 656, 733, 1099
Bse1I ACTGG 2 cut(s) 339, 1244
Bse21I CCTNAGG 2 cut(s) 873, 1389
BseAI TCCGGA 1 cut(s) 1129
BseBI CCWGG 1 cut(s) 587
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 585, 754, 2024
BseGI GGATG 7 cut(s) 312, 343, 742, 823, 1473, 1587, 1610
BseLI CCNNNNNNNGG 5 cut(s) 382, 534, 656, 733, 1099
BseMII CTCAG 7 cut(s) 402, 1380, 1788, 1815, 1842, 1869, 2004
BseNI ACTGG 2 cut(s) 339, 1244
BseRI GAGGAG 2 cut(s) 428, 1013
BseSI GKGCMC 1 cut(s) 594
BseXI GCAGC 4 cut(s) 838, 841, 1090, 1226
BsgI GTGCAG 1 cut(s) 845
Bsh1236I CGCG 1 cut(s) 864
BshFI GGCC 6 cut(s) 149, 233, 726, 753, 1092, 1787
BshNI GGYRCC 2 cut(s) 371, 1612
BshVI ATCGAT 1 cut(s) 201
BsiHKAI GWGCWC 4 cut(s) 47, 270, 594, 1420
BsiSI CCGG 4 cut(s) 376, 528, 1130, 1507
BslFI GGGAC 3 cut(s) 959, 1380, 1588
BslI CCNNNNNNNGG 5 cut(s) 382, 534, 656, 733, 1099
BsmAI GTCTC 3 cut(s) 48, 695, 995
BsmBI CGTCTC 3 cut(s) 48, 695, 995
BsmFI GGGAC 3 cut(s) 959, 1380, 1588
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 6 cut(s) 149, 233, 726, 753, 1092, 1787
Bsp1286I GDGCHC 5 cut(s) 47, 270, 524, 594, 1420
Bsp13I TCCGGA 1 cut(s) 1129
Bsp143I GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
BspACI CCGC 5 cut(s) 172, 379, 864, 882, 890
BspANI GGCC 6 cut(s) 149, 233, 726, 753, 1092, 1787
BspCNI CTCAG 7 cut(s) 403, 1381, 1789, 1816, 1843, 1870, 2005
BspDI ATCGAT 1 cut(s) 201
BspEI TCCGGA 1 cut(s) 1129
BspFNI CGCG 1 cut(s) 864
BspLI GGNNCC 6 cut(s) 148, 373, 1368, 1386, 1614, 1786
BspMI ACCTGC 2 cut(s) 451, 1318
BspPI GGATC 4 cut(s) 858, 1086, 1379, 1392
BspT107I GGYRCC 2 cut(s) 371, 1612
BsrI ACTGG 2 cut(s) 339, 1244
BssECI CCNNGG 3 cut(s) 585, 754, 2024
BssMI GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
BssNI GRCGYC 3 cut(s) 57, 372, 393
BssT1I CCWWGG 2 cut(s) 754, 2024
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 3 cut(s) 681, 786, 1313
Bst6I CTCTTC 3 cut(s) 127, 293, 1341
BstACI GRCGYC 3 cut(s) 57, 372, 393
BstC8I GCNNGC 5 cut(s) 47, 486, 1116, 1397, 1691
BstEII GGTNACC 1 cut(s) 645
BstF5I GGATG 7 cut(s) 312, 343, 742, 823, 1473, 1587, 1610
BstFNI CGCG 1 cut(s) 864
BstH2I RGCGCY 3 cut(s) 375, 835, 1115
BstHHI GCGC 4 cut(s) 374, 834, 864, 1114
BstKTI GATC 6 cut(s) 138, 853, 1081, 1387, 1531, 1735
BstMAI GTCTC 3 cut(s) 48, 695, 995
BstMBI GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
BstMWI GCNNNNNNNGC 3 cut(s) 861, 868, 1109
BstNI CCWGG 1 cut(s) 587
BstPI GGTNACC 1 cut(s) 645
BstSCI CCNGG 2 cut(s) 374, 585
BstSFI CTRYAG 5 cut(s) 1728, 1842, 1950, 1977, 2004
BstSLI GKGCMC 1 cut(s) 594
BstUI CGCG 1 cut(s) 864
BstV1I GCAGC 4 cut(s) 838, 841, 1090, 1226
BstX2I RGATCY 1 cut(s) 1384
BstYI RGATCY 1 cut(s) 1384
Bsu15I ATCGAT 1 cut(s) 201
Bsu36I CCTNAGG 2 cut(s) 873, 1389
BsuI GTATCC 3 cut(s) 1807, 1861, 1888
BsuRI GGCC 6 cut(s) 149, 233, 726, 753, 1092, 1787
BsuTUI ATCGAT 1 cut(s) 201
BtrI CACGTC 1 cut(s) 705
BtsCI GGATG 7 cut(s) 312, 343, 742, 823, 1473, 1587, 1610
BtsI GCAGTG 2 cut(s) 12, 2036
BtsIMutI CAGTG 3 cut(s) 12, 1641, 2036
BveI ACCTGC 2 cut(s) 451, 1318
Cac8I GCNNGC 5 cut(s) 47, 486, 1116, 1397, 1691
CaiI CAGNNNCTG 6 cut(s) 1796, 1823, 1850, 1877, 1958, 2012
CfoI GCGC 4 cut(s) 374, 834, 864, 1114
Cfr13I GGNCC 4 cut(s) 147, 724, 1367, 1785
ClaI ATCGAT 1 cut(s) 201
CseI GACGC 2 cut(s) 65, 1616
Csp6I GTAC 2 cut(s) 94, 1286
CviAII CATG 6 cut(s) 91, 1038, 1088, 1202, 1571, 1760
CviQI GTAC 2 cut(s) 94, 1286
DinI GGCGCC 1 cut(s) 373
DpnI GATC 6 cut(s) 137, 852, 1080, 1386, 1530, 1734
DpnII GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
DraI TTTAAA 1 cut(s) 1029
DrdI GACNNNNNNGTC 1 cut(s) 392
DriI GACNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 3 cut(s) 127, 293, 1341
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 3 cut(s) 127, 293, 1341
Ecl136II GAGCTC 2 cut(s) 45, 268
Eco130I CCWWGG 2 cut(s) 754, 2024
Eco24I GRGCYC 3 cut(s) 47, 270, 524
Eco47I GGWCC 1 cut(s) 1367
Eco53kI GAGCTC 2 cut(s) 45, 268
Eco57I CTGAAG 1 cut(s) 82
Eco81I CCTNAGG 2 cut(s) 873, 1389
Eco91I GGTNACC 1 cut(s) 645
EcoICRI GAGCTC 2 cut(s) 45, 268
EcoO65I GGTNACC 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 585
EcoT14I CCWWGG 2 cut(s) 754, 2024
EcoT38I GRGCYC 3 cut(s) 47, 270, 524
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
ErhI CCWWGG 2 cut(s) 754, 2024
Esp3I CGTCTC 3 cut(s) 48, 695, 995
FaeI CATG 6 cut(s) 94, 1041, 1091, 1205, 1574, 1763
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 1316, 1348
FaqI GGGAC 3 cut(s) 959, 1380, 1588
FatI CATG 6 cut(s) 90, 1037, 1087, 1201, 1570, 1759
FauI CCCGC 1 cut(s) 179
Fnu4HI GCNGC 5 cut(s) 380, 827, 830, 1104, 1240
FokI GGATG 7 cut(s) 299, 350, 749, 830, 1480, 1574, 1617
FriOI GRGCYC 3 cut(s) 47, 270, 524
Fsp4HI GCNGC 5 cut(s) 380, 827, 830, 1104, 1240
FspBI CTAG 1 cut(s) 903
GlaI GCGC 4 cut(s) 373, 833, 863, 1113
GluI GCNGC 5 cut(s) 380, 827, 830, 1104, 1240
GsuI CTGGAG 2 cut(s) 377, 1006
HaeII RGCGCY 3 cut(s) 375, 835, 1115
HaeIII GGCC 6 cut(s) 149, 233, 726, 753, 1092, 1787
HapII CCGG 4 cut(s) 376, 528, 1130, 1507
HgaI GACGC 2 cut(s) 65, 1616
HhaI GCGC 4 cut(s) 374, 834, 864, 1114
Hin1I GRCGYC 3 cut(s) 57, 372, 393
Hin1II CATG 6 cut(s) 94, 1041, 1091, 1205, 1574, 1763
Hin6I GCGC 4 cut(s) 372, 832, 862, 1112
HinP1I GCGC 4 cut(s) 372, 832, 862, 1112
HincII GTYRAC 2 cut(s) 912, 1504
HindII GTYRAC 2 cut(s) 912, 1504
HindIII AAGCTT 1 cut(s) 1277
HpaI GTTAAC 2 cut(s) 912, 1504
HpaII CCGG 4 cut(s) 376, 528, 1130, 1507
HphI GGTGA 2 cut(s) 1226, 1505
Hpy188I TCNGA 7 cut(s) 67, 101, 193, 398, 807, 1357, 1533
Hpy188III TCNNGA 9 cut(s) 40, 53, 316, 734, 1130, 1142, 1165, 1388, 1886
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 6 cut(s) 136, 343, 1103, 1162, 1230, 1883
HpyCH4III ACNGT 3 cut(s) 681, 786, 1313
HpyCH4IV ACGT 5 cut(s) 12, 393, 704, 1159, 1962
HpyCH4V TGCA 6 cut(s) 17, 261, 592, 708, 826, 1118
HpyF10VI GCNNNNNNNGC 3 cut(s) 861, 868, 1109
HpySE526I ACGT 5 cut(s) 12, 393, 704, 1159, 1962
Hsp92I GRCGYC 3 cut(s) 57, 372, 393
Hsp92II CATG 6 cut(s) 94, 1041, 1091, 1205, 1574, 1763
HspAI GCGC 4 cut(s) 372, 832, 862, 1112
KasI GGCGCC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 1129
KspAI GTTAAC 2 cut(s) 912, 1504
Kzo9I GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
LmnI GCTCC 4 cut(s) 265, 352, 987, 1132
Lsp1109I GCAGC 4 cut(s) 838, 841, 1090, 1226
LweI GCATC 1 cut(s) 214
MaeI CTAG 1 cut(s) 903
MaeII ACGT 5 cut(s) 12, 393, 704, 1159, 1962
MaeIII GTNAC 4 cut(s) 645, 898, 1040, 1636
MalI GATC 6 cut(s) 137, 852, 1080, 1386, 1530, 1734
MboI GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
MboII GAAGA 8 cut(s) 114, 238, 280, 331, 686, 950, 1021, 1358
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 1 cut(s) 1384
MhlI GDGCHC 5 cut(s) 47, 270, 524, 594, 1420
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 4 cut(s) 71, 1160, 1346, 1553
MmeI TCCRAC 3 cut(s) 45, 376, 795
MroI TCCGGA 1 cut(s) 1129
MslI CAYNNNNRTG 2 cut(s) 587, 1706
MspA1I CMGCKG 1 cut(s) 1689
MspI CCGG 4 cut(s) 376, 528, 1130, 1507
MspR9I CCNGG 2 cut(s) 376, 587
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 587
MvnI CGCG 1 cut(s) 864
MwoI GCNNNNNNNGC 3 cut(s) 861, 868, 1109
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 1 cut(s) 376
NdeII GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
NlaIII CATG 6 cut(s) 94, 1041, 1091, 1205, 1574, 1763
NlaIV GGNNCC 6 cut(s) 148, 373, 1368, 1386, 1614, 1786
NmeAIII GCCGAG 2 cut(s) 85, 259
NmuCI GTSAC 3 cut(s) 898, 1040, 1636
OliI CACNNNNGTG 1 cut(s) 587
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 6 cut(s) 243, 1122, 1138, 1358, 1510, 1882
PflMI CCANNNNNTGG 1 cut(s) 733
PkrI GCNGC 5 cut(s) 381, 828, 831, 1105, 1241
PleI GAGTC 4 cut(s) 70, 1159, 1346, 1552
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 4 cut(s) 70, 1159, 1346, 1552
PsiI TTATAA 1 cut(s) 1478
Psp124BI GAGCTC 2 cut(s) 47, 270
Psp1406I AACGTT 1 cut(s) 1159
Psp6I CCWGG 1 cut(s) 585
PspEI GGTNACC 1 cut(s) 645
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 6 cut(s) 148, 373, 1368, 1386, 1614, 1786
PspPI GGNCC 4 cut(s) 147, 724, 1367, 1785
PstNI CAGNNNCTG 6 cut(s) 1796, 1823, 1850, 1877, 1958, 2012
PsuI RGATCY 1 cut(s) 1384
PvuII CAGCTG 1 cut(s) 1689
RsaI GTAC 2 cut(s) 95, 1287
RsaNI GTAC 2 cut(s) 94, 1286
RseI CAYNNNNRTG 2 cut(s) 587, 1706
SacI GAGCTC 2 cut(s) 47, 270
SatI GCNGC 5 cut(s) 380, 827, 830, 1104, 1240
Sau3AI GATC 6 cut(s) 135, 850, 1078, 1384, 1528, 1732
Sau96I GGNCC 4 cut(s) 147, 724, 1367, 1785
SchI GAGTC 4 cut(s) 71, 1160, 1346, 1553
ScrFI CCNGG 2 cut(s) 376, 587
SduI GDGCHC 5 cut(s) 47, 270, 524, 594, 1420
SfaNI GCATC 1 cut(s) 214
SfcI CTRYAG 5 cut(s) 1728, 1842, 1950, 1977, 2004
SfoI GGCGCC 1 cut(s) 373
SinI GGWCC 1 cut(s) 1367
SmiMI CAYNNNNRTG 2 cut(s) 587, 1706
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
SpeI ACTAGT 1 cut(s) 902
SsiI CCGC 5 cut(s) 172, 379, 864, 882, 890
SspDI GGCGCC 1 cut(s) 371
SspMI CTAG 1 cut(s) 903
SstI GAGCTC 2 cut(s) 47, 270
StyD4I CCNGG 2 cut(s) 374, 585
StyI CCWWGG 2 cut(s) 754, 2024
TaaI ACNGT 3 cut(s) 681, 786, 1313
TaiI ACGT 5 cut(s) 15, 396, 707, 1162, 1965
TaqI TCGA 7 cut(s) 52, 134, 201, 331, 714, 925, 1227
TatI WGTACW 2 cut(s) 93, 1285
TauI GCSGC 1 cut(s) 382
TfiI GAWTC 6 cut(s) 243, 1122, 1138, 1358, 1510, 1882
TscAI CASTG 3 cut(s) 12, 1641, 2036
TseFI GTSAC 3 cut(s) 898, 1040, 1636
TseI GCWGC 4 cut(s) 826, 829, 1103, 1239
Tsp45I GTSAC 3 cut(s) 898, 1040, 1636
TspDTI ATGAA 8 cut(s) 301, 338, 1022, 1026, 1083, 1203, 1287, 1748
TspRI CASTG 3 cut(s) 12, 1641, 2036
Van91I CCANNNNNTGG 1 cut(s) 733
VneI GTGCAC 1 cut(s) 590
VpaK11BI GGWCC 1 cut(s) 1367
XapI RAATTY 3 cut(s) 20, 978, 1301
XcmI CCANNNNNNNNNTGG 2 cut(s) 734, 788
XspI CTAG 1 cut(s) 903
ZraI GACGTC 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.