Rh2AG490300

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000202
Physical Location & Seq
Forward (+)
71216669 .. 71251393
34725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG490300.1

Sequence Viewer

Length: 2028 bp
ATGAAGAGCCGGGTGACCACACTGAAAAGGAAGTTTGTGATTTTGGTCTCTAATGCAAGGGAGCAGAAAGAGAGGAACAACACCGTCAAACGTTTGGGACTACTTACTCAAGAGAAAGTGGCTGTAAAGAAGAATTTAGAAGAAATAGTAGTACTTCCTAATGCAAGTACACTGGAGATTCATGAATTGCTTGTTGATACAGATCGAGTTGGATTTACTCCAATTGGAATAGTAGGCATGGCTGGCGTGGAAAAAGAACTAGTAGATAGTGCTAGTGCCACTTGGGTCAGCATCAGCATTGTGACATGCATACTTGACAAGTTGGGCGAGAAAGAAACAAGTATTTCTGGACTCGGCCTTGATTCACTCTTGGAAAGGCTCAACCATATTTTATCGGCTAAAAGGTATCTGATTGTGTTGGATGATGTGTGGCATACTATGGAATTCTACTCGGATATAGGTCATAATCTTCCAGAAGGTGAGAAATCTGGTGATTGCCTATCACAAGGATTACCTAAGCATTGTGGTGGTGCGGTCATTGTCACTAGTAGGATAAGAGAGGTGGCTCAAGACATGGTAGGCCAGAATAATGTATTTCTTGCTAGGCCTTGGGACAAAGGATGGTCCTGGATGCACTTCAGGTCCTTTGTCACAACGAAAGCAGAAGGTTTCGGTGAAGAAAAACGTTTTAGCATGTCAAATTGGGAAATAGTGCAGAAGATTGAGAATGAAATTCTGGATCAAATAGGTGGTCTACCATCAATTGCTAAGGCACTCGCAGAACTAATAGTTGAAAAACGGATTATTGAGACTGATGATAAGGGGTCTGCCCTGAAGGAGATGTTGATACCTCATGAATTTCTTATTCATCGTTCATACATATTTGTTCCAGTCAATGAAAGGAACATAAAATGGATTGCTGATAAAACTGACATACCAAAATGCCCAGTCTTCGTGTTTGTTGATTTAAAAATGGAATTCTTTGCAGTAAGACTTCTCGAAGAATTTCGCTATCAGCTTAATCAAGATCAGGTTGTTGCTGTTCTAGACCAGAGTTTCATTAATAGGCAAAAGATAAAGACAGAGGAACCAGAAAAGGCGCTAAAAGAGTTTTATGGTATTCTCGAAAATCTTAAGTGCAAAGGACATAAATTTGCTGTTGAAATTCAAAAGAAGATGACAATTATGGAGTTTGATGCTGATAAAGTACCAGTGAACAAGATTTTGGGGATGGTACTAAATCATGCAAAGCAGATGAAAACAGACAGCTGGCATATCCTCATTAAGGTGAAAGCGACAGCTCATCAACAAATACCCTATTGTTTGCATTTATTTCATGAAGCAGATATGGAAAATGAGGATAATCCGACATTAATATACGGAGGATTTTGGAACTACTTCAGCTTGGGAGTCGATGACCCAAGGTTGTATGGAGCTTCCCGCTTTCGTCATACTTCAAGGACCTTAAGCATCGCACTTGCAAAAGTAGAGGTTTTGAAACATCATGGTGATCAGTGGGAGGTGCTTAGCATTCCTAGTAGCGTCAGATCAATTGTTTGTCTTAACTTGCCTAGTTTTCTTGGTGGGCTAACTCCTTGGACAACAGCAGATATAAAGAAAGATCAAACAAGAGGCCTGTCCTCATCTTTCATAGATGATGGATATCTTGAAATTATTGGTTCAAAAGATGATTTACTTTCCCAGAAGAAAAGGTGGATTCGCCTTGATCAGGTACAAGAAATGCGCTTCAAGTTTATTGAGGATTCAGATGCGGTTGTAAACATGACAATCGATGGGGTACAGTTGAAAAGAACTTCACTTGATGGTTTACTCGAGATTGAAATCTCTTACCAGTGTCAAGTTAATGTTCTTGTTAAACAAGACTACTCAGCCAAAAATACCCATGCCTCATCGCAGCCCTGTGGTTCAGAAACTGATGATGATAATGATAGAGAGTATTCTCAAAGCTATAAGAAATTTGGGGCAGCAAGCAGCTTTAAAATGCCGGAGGATGACATTAGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

675

Amino Acids

76.74

Weight (kDa)

5.97

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 92 - 220 9.8e-09 NB-ARC domain
DAGK_acc PF00609 504 - 599 1.2e-08 Diacylglycerol kinase accessory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 754
AciI CCGC 3 cut(s) 533, 1441, 1772
AclI AACGTT 2 cut(s) 91, 685
AclWI GGATC 1 cut(s) 747
AcsI RAATTY 9 cut(s) 133, 443, 732, 857, 977, 1004, 1151, 1164, 1976
AcuI CTGAAG 3 cut(s) 622, 854, 1384
AfaI GTAC 6 cut(s) 153, 169, 1209, 1236, 1734, 1800
AfiI CCNNNNNNNGG 2 cut(s) 1285, 1729
AflII CTTAAG 2 cut(s) 1133, 1465
AhlI ACTAGT 2 cut(s) 259, 545
AjnI CCWGG 1 cut(s) 626
AjuI GAANNNNNNNTTGG 2 cut(s) 1208, 1240
AluBI AGCT 7 cut(s) 1018, 1269, 1301, 1404, 1436, 1968, 1995
AluI AGCT 7 cut(s) 1018, 1269, 1301, 1404, 1436, 1968, 1995
Alw26I GTCTC 2 cut(s) 52, 803
AlwI GGATC 1 cut(s) 747
AlwNI CAGNNNCTG 1 cut(s) 1934
Ama87I CYCGRG 1 cut(s) 1832
AoxI GGCC 4 cut(s) 355, 580, 605, 1633
ApeKI GCWGC 3 cut(s) 1915, 1985, 1992
ApoI RAATTY 9 cut(s) 133, 443, 732, 857, 977, 1004, 1151, 1164, 1976
ArsI GACNNNNNNTTYG 2 cut(s) 736, 768
AseI ATTAAT 2 cut(s) 1062, 1373
Asp700I GAANNNNTTC 2 cut(s) 1005, 1397
AspLEI GCGC 2 cut(s) 1102, 1746
AspS9I GGNCC 3 cut(s) 624, 642, 1461
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 6 cut(s) 25, 491, 503, 686, 1300, 1520
AvaI CYCGRG 1 cut(s) 1832
AvaII GGWCC 3 cut(s) 624, 642, 1461
BbsI GAAGAC 1 cut(s) 943
BbvI GCAGC 3 cut(s) 1927, 1997, 2004
BccI CCATC 6 cut(s) 615, 766, 1225, 1652, 1787, 1817
BciT130I CCWGG 1 cut(s) 628
BclI TGATCA 2 cut(s) 1510, 1726
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 2 cut(s) 52, 803
BcuI ACTAGT 2 cut(s) 259, 545
BfaI CTAG 7 cut(s) 260, 273, 546, 603, 1046, 1536, 1572
BfoI RGCGCY 1 cut(s) 1103
BfrI CTTAAG 2 cut(s) 1133, 1465
BisI GCNGC 3 cut(s) 1916, 1986, 1993
BlpI GCTNAGC 1 cut(s) 1526
BlsI GCNGC 3 cut(s) 1917, 1987, 1994
BmcAI AGTACT 1 cut(s) 153
Bme1390I CCNGG 2 cut(s) 11, 628
Bme18I GGWCC 3 cut(s) 624, 642, 1461
BmeT110I CYCGRG 1 cut(s) 1832
BmgT120I GGNCC 3 cut(s) 624, 642, 1461
BmiI GGNNCC 1 cut(s) 1089
BmrFI CCNGG 2 cut(s) 11, 628
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 5 cut(s) 300, 621, 1186, 1479, 1759
BmuI ACTGGG 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 943
BpmI CTGGAG 1 cut(s) 194
Bpu10I CCTNAGC 2 cut(s) 516, 768
Bpu1102I GCTNAGC 1 cut(s) 1526
BpuEI CTTGAG 2 cut(s) 93, 552
BpuMI CCSGG 1 cut(s) 11
Bsa29I ATCGAT 1 cut(s) 1791
BsaBI GATNNNNATC 3 cut(s) 201, 1662, 1841
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 3 cut(s) 608, 1421, 1595
Bsc4I CCNNNNNNNGG 2 cut(s) 1285, 1729
Bse1I ACTGG 5 cut(s) 177, 890, 947, 1211, 1852
Bse8I GATNNNNATC 3 cut(s) 201, 1662, 1841
BseBI CCWGG 1 cut(s) 628
BseCI ATCGAT 1 cut(s) 1791
BseDI CCNNGG 3 cut(s) 608, 1421, 1595
BseGI GGATG 5 cut(s) 427, 626, 636, 1236, 2017
BseJI GATNNNNATC 3 cut(s) 201, 1662, 1841
BseLI CCNNNNNNNGG 2 cut(s) 1285, 1729
BseMII CTCAG 1 cut(s) 1902
BseNI ACTGG 5 cut(s) 177, 890, 947, 1211, 1852
BseXI GCAGC 3 cut(s) 1927, 1997, 2004
BsgI GTGCAG 1 cut(s) 734
BshFI GGCC 4 cut(s) 357, 582, 607, 1635
BshVI ATCGAT 1 cut(s) 1791
BsiHKCI CYCGRG 1 cut(s) 1832
BsiSI CCGG 2 cut(s) 10, 2006
BslFI GGGAC 2 cut(s) 111, 626
BslI CCNNNNNNNGG 2 cut(s) 1285, 1729
BsmAI GTCTC 2 cut(s) 52, 803
BsmFI GGGAC 2 cut(s) 111, 626
BsmI GAATGC 1 cut(s) 1530
BsnI GGCC 4 cut(s) 357, 582, 607, 1635
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 1832
Bsp143I GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
Bsp1720I GCTNAGC 1 cut(s) 1526
BspACI CCGC 3 cut(s) 533, 1441, 1772
BspANI GGCC 4 cut(s) 357, 582, 607, 1635
BspCNI CTCAG 1 cut(s) 1901
BspDI ATCGAT 1 cut(s) 1791
BspHI TCATGA 3 cut(s) 181, 853, 1336
BspLI GGNNCC 1 cut(s) 1089
BspPI GGATC 1 cut(s) 747
BspTI CTTAAG 2 cut(s) 1133, 1465
BspTNI GGTCTC 1 cut(s) 52
BsrI ACTGG 5 cut(s) 177, 890, 947, 1211, 1852
BssECI CCNNGG 3 cut(s) 608, 1421, 1595
BssMI GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
BssT1I CCWWGG 3 cut(s) 608, 1421, 1595
Bst2UI CCWGG 1 cut(s) 628
Bst4CI ACNGT 2 cut(s) 85, 1803
BstAFI CTTAAG 2 cut(s) 1133, 1465
BstC8I GCNNGC 3 cut(s) 244, 1271, 1990
BstDEI CTNAG 4 cut(s) 516, 768, 1526, 1888
BstEII GGTNACC 1 cut(s) 13
BstENI CCTNNNNNAGG 2 cut(s) 1283, 1727
BstF5I GGATG 5 cut(s) 427, 626, 636, 1236, 2017
BstH2I RGCGCY 1 cut(s) 1103
BstHHI GCGC 2 cut(s) 1102, 1746
BstKTI GATC 7 cut(s) 205, 742, 1030, 1513, 1550, 1624, 1729
BstMAI GTCTC 2 cut(s) 52, 803
BstMBI GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
BstMWI GCNNNNNNNGC 1 cut(s) 243
BstNI CCWGG 1 cut(s) 628
BstNSI RCATGY 2 cut(s) 309, 697
BstPI GGTNACC 1 cut(s) 13
BstSCI CCNGG 2 cut(s) 9, 626
BstV1I GCAGC 3 cut(s) 1927, 1997, 2004
BstV2I GAAGAC 1 cut(s) 943
Bsu15I ATCGAT 1 cut(s) 1791
BsuRI GGCC 4 cut(s) 357, 582, 607, 1635
BsuTUI ATCGAT 1 cut(s) 1791
BtgZI GCGATG 2 cut(s) 1456, 1896
BtsCI GGATG 5 cut(s) 427, 626, 636, 1236, 2017
BtsIMutI CAGTG 5 cut(s) 20, 170, 1218, 1520, 1859
Cac8I GCNNGC 3 cut(s) 244, 1271, 1990
CaiI CAGNNNCTG 1 cut(s) 1934
CciI TCATGA 3 cut(s) 181, 853, 1336
CfoI GCGC 2 cut(s) 1102, 1746
Cfr13I GGNCC 3 cut(s) 624, 642, 1461
ClaI ATCGAT 1 cut(s) 1791
CseI GACGC 1 cut(s) 1531
Csp6I GTAC 6 cut(s) 152, 168, 1208, 1235, 1733, 1799
CviQI GTAC 6 cut(s) 152, 168, 1208, 1235, 1733, 1799
DdeI CTNAG 4 cut(s) 516, 768, 1526, 1888
DpnI GATC 7 cut(s) 204, 741, 1029, 1512, 1549, 1623, 1728
DpnII GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
DraI TTTAAA 2 cut(s) 969, 1999
Eco130I CCWWGG 3 cut(s) 608, 1421, 1595
Eco147I AGGCCT 2 cut(s) 607, 1635
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 1664
Eco47I GGWCC 3 cut(s) 624, 642, 1461
Eco57I CTGAAG 3 cut(s) 622, 854, 1384
Eco88I CYCGRG 1 cut(s) 1832
Eco91I GGTNACC 1 cut(s) 13
EcoNI CCTNNNNNAGG 2 cut(s) 1283, 1727
EcoO109I RGGNCCY 2 cut(s) 642, 1461
EcoO65I GGTNACC 1 cut(s) 13
EcoRI GAATTC 2 cut(s) 443, 977
EcoRII CCWGG 1 cut(s) 626
EcoRV GATATC 1 cut(s) 1664
EcoT14I CCWWGG 3 cut(s) 608, 1421, 1595
EcoT22I ATGCAT 1 cut(s) 311
ErhI CCWWGG 3 cut(s) 608, 1421, 1595
FaqI GGGAC 2 cut(s) 111, 626
FauI CCCGC 1 cut(s) 1448
FbaI TGATCA 2 cut(s) 1510, 1726
FblI GTMKAC 1 cut(s) 754
Fnu4HI GCNGC 3 cut(s) 1916, 1986, 1993
FokI GGATG 5 cut(s) 434, 633, 643, 1243, 2024
Fsp4HI GCNGC 3 cut(s) 1916, 1986, 1993
FspBI CTAG 7 cut(s) 260, 273, 546, 603, 1046, 1536, 1572
GlaI GCGC 2 cut(s) 1101, 1745
GluI GCNGC 3 cut(s) 1916, 1986, 1993
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 1103
HaeIII GGCC 4 cut(s) 357, 582, 607, 1635
HapII CCGG 2 cut(s) 10, 2006
HgaI GACGC 1 cut(s) 1531
HhaI GCGC 2 cut(s) 1102, 1746
Hin6I GCGC 2 cut(s) 1100, 1744
HinP1I GCGC 2 cut(s) 1100, 1744
HinfI GANTC 6 cut(s) 178, 351, 362, 1410, 1717, 1763
HpaII CCGG 2 cut(s) 10, 2006
HphI GGTGA 6 cut(s) 25, 491, 503, 686, 1300, 1520
Hpy166II GTNNAC 5 cut(s) 170, 755, 1216, 1780, 1829
Hpy188I TCNGA 6 cut(s) 411, 454, 1368, 1547, 1768, 1930
Hpy8I GTNNAC 5 cut(s) 170, 755, 1216, 1780, 1829
HpyAV CCTTC 3 cut(s) 470, 659, 829
HpyCH4III ACNGT 2 cut(s) 85, 1803
HpyCH4IV ACGT 2 cut(s) 91, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 243
HpyF3I CTNAG 4 cut(s) 516, 768, 1526, 1888
HpySE526I ACGT 2 cut(s) 91, 685
HspAI GCGC 2 cut(s) 1100, 1744
Ksp22I TGATCA 2 cut(s) 1510, 1726
Kzo9I GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
LmnI GCTCC 2 cut(s) 61, 1433
Lsp1109I GCAGC 3 cut(s) 1927, 1997, 2004
LweI GCATC 5 cut(s) 300, 621, 1186, 1479, 1759
MaeI CTAG 7 cut(s) 260, 273, 546, 603, 1046, 1536, 1572
MaeII ACGT 2 cut(s) 91, 685
MaeIII GTNAC 4 cut(s) 13, 301, 541, 649
MalI GATC 7 cut(s) 204, 741, 1029, 1512, 1549, 1623, 1728
MboI GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
MfeI CAATTG 3 cut(s) 222, 762, 1551
MlyI GAGTC 2 cut(s) 345, 1419
MmeI TCCRAC 3 cut(s) 190, 399, 1391
Mph1103I ATGCAT 1 cut(s) 311
MroXI GAANNNNTTC 2 cut(s) 1005, 1397
MslI CAYNNNNRTG 3 cut(s) 525, 1286, 1506
MspA1I CMGCKG 1 cut(s) 1269
MspCI CTTAAG 2 cut(s) 1133, 1465
MspI CCGG 2 cut(s) 10, 2006
MspR9I CCNGG 2 cut(s) 11, 628
MunI CAATTG 3 cut(s) 222, 762, 1551
Mva1269I GAATGC 1 cut(s) 1530
MvaI CCWGG 1 cut(s) 628
MwoI GCNNNNNNNGC 1 cut(s) 243
NciI CCSGG 1 cut(s) 11
NdeII GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
NlaIV GGNNCC 1 cut(s) 1089
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 4 cut(s) 13, 301, 541, 649
NsiI ATGCAT 1 cut(s) 311
NspI RCATGY 2 cut(s) 309, 697
PaeR7I CTCGAG 1 cut(s) 1832
PagI TCATGA 3 cut(s) 181, 853, 1336
PceI AGGCCT 2 cut(s) 607, 1635
PctI GAATGC 1 cut(s) 1530
PdmI GAANNNNTTC 2 cut(s) 1005, 1397
PfeI GAWTC 4 cut(s) 178, 362, 1717, 1763
PfoI TCCNGGA 1 cut(s) 626
PkrI GCNGC 3 cut(s) 1917, 1987, 1994
PleI GAGTC 2 cut(s) 345, 1418
PpsI GAGTC 2 cut(s) 345, 1418
PpuMI RGGWCCY 2 cut(s) 642, 1461
PshBI ATTAAT 2 cut(s) 1062, 1373
Psp1406I AACGTT 2 cut(s) 91, 685
Psp5II RGGWCCY 2 cut(s) 642, 1461
Psp6I CCWGG 1 cut(s) 626
PspEI GGTNACC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 626
PspN4I GGNNCC 1 cut(s) 1089
PspPI GGNCC 3 cut(s) 624, 642, 1461
PspPPI RGGWCCY 2 cut(s) 642, 1461
PstNI CAGNNNCTG 1 cut(s) 1934
PvuII CAGCTG 1 cut(s) 1269
RsaI GTAC 6 cut(s) 153, 169, 1209, 1236, 1734, 1800
RsaNI GTAC 6 cut(s) 152, 168, 1208, 1235, 1733, 1799
RseI CAYNNNNRTG 3 cut(s) 525, 1286, 1506
SatI GCNGC 3 cut(s) 1916, 1986, 1993
Sau3AI GATC 7 cut(s) 202, 739, 1027, 1510, 1547, 1621, 1726
Sau96I GGNCC 3 cut(s) 624, 642, 1461
ScaI AGTACT 1 cut(s) 153
SchI GAGTC 2 cut(s) 345, 1419
ScrFI CCNGG 2 cut(s) 11, 628
SfaNI GCATC 5 cut(s) 300, 621, 1186, 1479, 1759
Sfr274I CTCGAG 1 cut(s) 1832
SinI GGWCC 3 cut(s) 624, 642, 1461
SlaI CTCGAG 1 cut(s) 1832
SmiMI CAYNNNNRTG 3 cut(s) 525, 1286, 1506
SmlI CTYRAG 5 cut(s) 108, 567, 1133, 1465, 1832
SmoI CTYRAG 5 cut(s) 108, 567, 1133, 1465, 1832
SpeI ACTAGT 2 cut(s) 259, 545
SseBI AGGCCT 2 cut(s) 607, 1635
SsiI CCGC 3 cut(s) 533, 1441, 1772
SspMI CTAG 7 cut(s) 260, 273, 546, 603, 1046, 1536, 1572
StuI AGGCCT 2 cut(s) 607, 1635
StyD4I CCNGG 2 cut(s) 9, 626
StyI CCWWGG 3 cut(s) 608, 1421, 1595
TaaI ACNGT 2 cut(s) 85, 1803
TaiI ACGT 2 cut(s) 94, 688
TaqI TCGA 6 cut(s) 205, 999, 1125, 1413, 1791, 1833
TatI WGTACW 2 cut(s) 151, 167
TfiI GAWTC 4 cut(s) 178, 362, 1717, 1763
TscAI CASTG 5 cut(s) 27, 177, 1218, 1520, 1859
TseFI GTSAC 4 cut(s) 13, 301, 541, 649
TseI GCWGC 3 cut(s) 1915, 1985, 1992
Tsp45I GTSAC 4 cut(s) 13, 301, 541, 649
TspGWI ACGGA 2 cut(s) 814, 1395
TspRI CASTG 5 cut(s) 27, 177, 1218, 1520, 1859
Vha464I CTTAAG 2 cut(s) 1133, 1465
VpaK11BI GGWCC 3 cut(s) 624, 642, 1461
VspI ATTAAT 2 cut(s) 1062, 1373
XagI CCTNNNNNAGG 2 cut(s) 1283, 1727
XapI RAATTY 9 cut(s) 133, 443, 732, 857, 977, 1004, 1151, 1164, 1976
XbaI TCTAGA 1 cut(s) 1045
XceI RCATGY 2 cut(s) 309, 697
XhoI CTCGAG 1 cut(s) 1832
XmiI GTMKAC 1 cut(s) 754
XmnI GAANNNNTTC 2 cut(s) 1005, 1397
XspI CTAG 7 cut(s) 260, 273, 546, 603, 1046, 1536, 1572
ZrmI AGTACT 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.