Rmu_sc0001708.1_g000016

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001708.1
OG0000202
Physical Location & Seq
Forward (+)
57848 .. 60192
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001708.1_g000016.1.cds

Sequence Viewer

Length: 987 bp
atggaggagttgctagttgggcgttctagttctagttctagcggcgtgacatttatggaaattggggtaattggcattgctggcgtgggtaagaccaccctgctcattaacattatggaacgcttagtcagtggattacctaaggctagtggtgatgctgtcattgtcactagtagattgaaggaagtggctcaaaagcttgggaaacagatcactttgttcgtgagcctctgccaggaaaagattgctcgttatttgatgcatactattataagttctgtgtcttacggcattctattagaagtttttgaatttcgtgacgaatcaaagcagattgaggacttgctactccttgaagagggtcctatttctagcagtgctcgtaggttgagggcaattgggatatttggcactgctggggtgggtaagaccaccctggtgcagaaggttttggagagccagagagtgaaggacaagtttaagcgcactttcgttgcccttttgggctatttgattgtgttggatgatgtgtgtcatcagcacattaacattatggaacgctttgtcagtggattgcccaaggctagtggtgttgctctcattgtcactagtagattgagggaagtggctcaaaagcttggggaacgagaacacctgattcatgttcagccacaggaagaggtttgctcgcaaatgcttgataagaaattctcaagcatctcacaaggcgaaatcaacgaaatgtttgaaaaatcccttaccttggcaacttgcactctttatgtatctttgttgcctcagaaaatcacctcggcctatacagccgagggatcaaggaacaaacttgagtggaattcggcctcctcttggctcagtgactctatcttgattcggcttcaatacttatcgtcggctgacttgacgctggactctatccggattggctttgatggacgtcatcaacggctgcaattaatcttgaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

328

Amino Acids

36.34

Weight (kDa)

8.79

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
AatII GACGTC 1 cut(s) 958
AccIII TCCGGA 1 cut(s) 936
AciI CCGC 1 cut(s) 42
AclWI GGATC 1 cut(s) 838
AcsI RAATTY 3 cut(s) 311, 707, 853
AcyI GRCGYC 1 cut(s) 955
AfiI CCNNNNNNNGG 4 cut(s) 235, 358, 763, 867
AgsI TTSAA 6 cut(s) 181, 311, 356, 749, 899, 982
AhlI ACTAGT 2 cut(s) 170, 608
AjnI CCWGG 2 cut(s) 234, 435
AleI CACNNNNGTG 1 cut(s) 437
AluBI AGCT 2 cut(s) 199, 637
AluI AGCT 2 cut(s) 199, 637
Alw21I GWGCWC 1 cut(s) 382
AlwI GGATC 1 cut(s) 838
Aor13HI TCCGGA 1 cut(s) 936
AoxI GGCC 2 cut(s) 813, 858
ApeKI GCWGC 1 cut(s) 967
ApoI RAATTY 3 cut(s) 311, 707, 853
AseI ATTAAT 1 cut(s) 974
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 1 cut(s) 362
AsuHPI GGTGA 2 cut(s) 164, 799
AvaII GGWCC 1 cut(s) 362
AxyI CCTNAGG 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 1 cut(s) 954
BccI CCATC 1 cut(s) 944
BceAI ACGGC 2 cut(s) 304, 980
BciT130I CCWGG 2 cut(s) 236, 437
BcuI ACTAGT 2 cut(s) 170, 608
BfaI CTAG 9 cut(s) 14, 27, 33, 39, 147, 171, 372, 585, 609
BisI GCNGC 2 cut(s) 43, 968
BlsI GCNGC 2 cut(s) 44, 969
Bme1390I CCNGG 2 cut(s) 236, 437
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 362
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 2 cut(s) 236, 437
BmsI GCATC 3 cut(s) 145, 249, 726
BplI GAGNNNNNCTC 2 cut(s) 671, 703
BpuEI CTTGAG 2 cut(s) 697, 866
BsaHI GRCGYC 1 cut(s) 955
BsaJI CCNNGG 5 cut(s) 435, 579, 762, 810, 825
BsaWI WCCGGW 1 cut(s) 936
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 358, 763, 867
Bse21I CCTNAGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 75
BseAI TCCGGA 1 cut(s) 936
BseBI CCWGG 2 cut(s) 236, 437
BseDI CCNNGG 5 cut(s) 435, 579, 762, 810, 825
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 4 cut(s) 235, 358, 763, 867
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 2 cut(s) 812, 886
BseRI GAGGAG 2 cut(s) 20, 853
BseXI GCAGC 1 cut(s) 954
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 461
BshFI GGCC 2 cut(s) 815, 860
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 1 cut(s) 937
BslI CCNNNNNNNGG 4 cut(s) 235, 358, 763, 867
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 2 cut(s) 815, 860
Bsp1286I GDGCHC 1 cut(s) 382
Bsp13I TCCGGA 1 cut(s) 936
Bsp143I GATC 2 cut(s) 210, 830
BspACI CCGC 1 cut(s) 42
BspANI GGCC 2 cut(s) 815, 860
BspCNI CTCAG 2 cut(s) 811, 885
BspEI TCCGGA 1 cut(s) 936
BspLI GGNNCC 1 cut(s) 363
BspPI GGATC 1 cut(s) 838
BsrDI GCAATG 1 cut(s) 75
BssECI CCNNGG 5 cut(s) 435, 579, 762, 810, 825
BssMI GATC 2 cut(s) 210, 830
BssNI GRCGYC 1 cut(s) 955
BssT1I CCWWGG 2 cut(s) 579, 762
Bst2UI CCWGG 2 cut(s) 236, 437
Bst6I CTCTTC 2 cut(s) 351, 672
BstACI GRCGYC 1 cut(s) 955
BstC8I GCNNGC 2 cut(s) 82, 689
BstDEI CTNAG 4 cut(s) 124, 141, 798, 872
BstENI CCTNNNNNAGG 2 cut(s) 233, 356
BstF5I GGATG 1 cut(s) 529
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 2 cut(s) 213, 833
BstMBI GATC 2 cut(s) 210, 830
BstMWI GCNNNNNNNGC 3 cut(s) 19, 81, 821
BstNI CCWGG 2 cut(s) 236, 437
BstSCI CCNGG 2 cut(s) 234, 435
BstV1I GCAGC 1 cut(s) 954
Bsu36I CCTNAGG 1 cut(s) 141
BsuRI GGCC 2 cut(s) 815, 860
BtsCI GGATG 1 cut(s) 529
BtsI GCAGTG 2 cut(s) 382, 411
BtsIMutI CAGTG 5 cut(s) 136, 382, 411, 574, 880
Cac8I GCNNGC 2 cut(s) 82, 689
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 362
CseI GACGC 1 cut(s) 931
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 1 cut(s) 662
DdeI CTNAG 4 cut(s) 124, 141, 798, 872
DpnI GATC 2 cut(s) 212, 832
DpnII GATC 2 cut(s) 210, 830
Eam1104I CTCTTC 2 cut(s) 351, 672
EarI CTCTTC 2 cut(s) 351, 672
Eco130I CCWWGG 2 cut(s) 579, 762
Eco47I GGWCC 1 cut(s) 362
Eco81I CCTNAGG 1 cut(s) 141
EcoNI CCTNNNNNAGG 2 cut(s) 233, 356
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 2 cut(s) 234, 435
EcoT14I CCWWGG 2 cut(s) 579, 762
EcoT22I ATGCAT 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 579, 762
FaeI CATG 1 cut(s) 665
FaiI YATR 8 cut(s) 56, 116, 264, 272, 554, 663, 783, 819
FatI CATG 1 cut(s) 661
Fnu4HI GCNGC 2 cut(s) 43, 968
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 2 cut(s) 43, 968
FspBI CTAG 9 cut(s) 14, 27, 33, 39, 147, 171, 372, 585, 609
GlaI GCGC 1 cut(s) 485
GluI GCNGC 2 cut(s) 43, 968
GsaI CCCAGC 1 cut(s) 420
HaeIII GGCC 2 cut(s) 815, 860
HapII CCGG 1 cut(s) 937
HgaI GACGC 1 cut(s) 931
HhaI GCGC 1 cut(s) 486
Hin1I GRCGYC 1 cut(s) 955
Hin1II CATG 1 cut(s) 665
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HindIII AAGCTT 2 cut(s) 197, 635
HinfI GANTC 5 cut(s) 323, 658, 878, 889, 929
HpaII CCGG 1 cut(s) 937
HphI GGTGA 2 cut(s) 164, 799
Hpy188I TCNGA 1 cut(s) 801
Hpy188III TCNNGA 5 cut(s) 223, 317, 886, 937, 979
Hpy99I CGWCG 1 cut(s) 913
HpyAV CCTTC 3 cut(s) 175, 439, 463
HpyCH4IV ACGT 1 cut(s) 955
HpyCH4V TGCA 4 cut(s) 262, 442, 774, 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 81, 821
HpyF3I CTNAG 4 cut(s) 124, 141, 798, 872
HpySE526I ACGT 1 cut(s) 955
Hsp92I GRCGYC 1 cut(s) 955
Hsp92II CATG 1 cut(s) 665
HspAI GCGC 1 cut(s) 484
Kpn2I TCCGGA 1 cut(s) 936
Kzo9I GATC 2 cut(s) 210, 830
Lsp1109I GCAGC 1 cut(s) 954
LweI GCATC 3 cut(s) 145, 249, 726
MaeI CTAG 9 cut(s) 14, 27, 33, 39, 147, 171, 372, 585, 609
MaeII ACGT 1 cut(s) 955
MaeIII GTNAC 5 cut(s) 46, 166, 317, 604, 875
MalI GATC 2 cut(s) 212, 832
MboI GATC 2 cut(s) 210, 830
MboII GAAGA 2 cut(s) 368, 689
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 7 cut(s) 60, 69, 311, 396, 707, 853, 971
MlyI GAGTC 2 cut(s) 872, 923
MmeI TCCRAC 1 cut(s) 501
Mph1103I ATGCAT 1 cut(s) 264
MroI TCCGGA 1 cut(s) 936
MseI TTAA 4 cut(s) 108, 480, 546, 974
MslI CAYNNNNRTG 1 cut(s) 437
MspI CCGG 1 cut(s) 937
MspR9I CCNGG 2 cut(s) 236, 437
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 291
MvaI CCWGG 2 cut(s) 236, 437
MwoI GCNNNNNNNGC 3 cut(s) 19, 81, 821
NdeII GATC 2 cut(s) 210, 830
NlaIII CATG 1 cut(s) 665
NlaIV GGNNCC 1 cut(s) 363
NmeAIII GCCGAG 2 cut(s) 791, 850
NmuCI GTSAC 5 cut(s) 46, 166, 317, 604, 875
NsiI ATGCAT 1 cut(s) 264
OliI CACNNNNGTG 1 cut(s) 437
PctI GAATGC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 323, 658, 889
PkrI GCNGC 2 cut(s) 44, 969
PleI GAGTC 2 cut(s) 872, 923
PpsI GAGTC 2 cut(s) 872, 923
PpuMI RGGWCCY 1 cut(s) 362
PshBI ATTAAT 1 cut(s) 974
PsiI TTATAA 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 234, 435
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 2 cut(s) 234, 435
PspN4I GGNNCC 1 cut(s) 363
PspPI GGNCC 1 cut(s) 362
PspPPI RGGWCCY 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 437
SaqAI TTAA 4 cut(s) 108, 480, 546, 974
SatI GCNGC 2 cut(s) 43, 968
Sau3AI GATC 2 cut(s) 210, 830
Sau96I GGNCC 1 cut(s) 362
SchI GAGTC 2 cut(s) 872, 923
ScrFI CCNGG 2 cut(s) 236, 437
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 3 cut(s) 145, 249, 726
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 712, 845
SmoI CTYRAG 2 cut(s) 712, 845
SpeI ACTAGT 2 cut(s) 170, 608
Sse9I AATT 7 cut(s) 60, 69, 311, 396, 707, 853, 971
SsiI CCGC 1 cut(s) 42
SspMI CTAG 9 cut(s) 14, 27, 33, 39, 147, 171, 372, 585, 609
StyD4I CCNGG 2 cut(s) 234, 435
StyI CCWWGG 2 cut(s) 579, 762
TaiI ACGT 1 cut(s) 958
TasI AATT 7 cut(s) 60, 69, 311, 396, 707, 853, 971
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 3 cut(s) 323, 658, 889
Tru1I TTAA 4 cut(s) 108, 480, 546, 974
Tru9I TTAA 4 cut(s) 108, 480, 546, 974
TscAI CASTG 5 cut(s) 136, 382, 418, 574, 880
TseFI GTSAC 5 cut(s) 46, 166, 317, 604, 875
TseI GCWGC 1 cut(s) 967
Tsp45I GTSAC 5 cut(s) 46, 166, 317, 604, 875
TspDTI ATGAA 1 cut(s) 650
TspRI CASTG 5 cut(s) 136, 382, 418, 574, 880
VpaK11BI GGWCC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 974
XagI CCTNNNNNAGG 2 cut(s) 233, 356
XapI RAATTY 3 cut(s) 311, 707, 853
XspI CTAG 9 cut(s) 14, 27, 33, 39, 147, 171, 372, 585, 609
ZraI GACGTC 1 cut(s) 956
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.