Rw2G017530

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000202
Physical Location & Seq
Reverse (-)
21649394 .. 21650569
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017530.1

Sequence Viewer

Length: 1176 bp
ATGGCATTGGATGTTGCTGAATTCCTGTGGAACAAGTTCTTGAAGTCTCTAGCAGAGCTAGAGTCTGAATCTAATTCAGGTTCTATTACCATCTCTGTCTTCAAATCATCTATAAAAGATCTCGTGTCTGAATTCAAAGCAATCAAAGATGTTGTGAATGAGAGAGATATAATGCCGAAACCTTATACCCGCTCAGAAATTATTGATGTGCTCTACAATCTCAACGATGCATTGGCCGAGTGCCGAGTCTTCGCACATGAGTGCATAGAAATAACTGATATGCACTCACGCTTCAACTTTCGAAGATTTACTTTCATCTCAAAGATAAAGGGTCGGCTGGCCACAATAAAACAGGAGCTTGAGGACTTGCCGCAACAACAAAATGGCAATCCTCGGGAAAAGACCGAATTCACTGGATCCGGACCTAAACTTTCAGCCAAAGTTTTCGGATTTGATGAGCAATTAAAGGAGATTAATGAAAAGTTGCTACAAGCAGGGGATTCCAGTTCTAGCAGTGCTGGTAAATTGACGGCAATTGGGGTAGTTGGCATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTGGAGAGCGAGGAAGTGATGAAGAAGTTTAATCATATACTGTGGCTGCCCTTGTCGGGTATGAGTGAAGGAGAACAACAATATAGTAGGTTTAGCTTTGAAACACGTATCTTCGATGGTAATGGCCGTACCATAGAGGAGAGGGTTGTTGGCCTTGATAAGCTCCTGGAAAGGCTAAGCCGCCTGCTGTTATCAGGTAAATGCTATTTGATTGTGTTGGATGATGTGTGTCATCGGCACATTAACATTATGGAGCGCTTATGCAGCGGACTGCCCAAGCATAATGGTAGTGCTATAATTGTCACTACTAGATTGAAGGAAGTAGCTCTGAAGCTGGGGAAACAACACGAGTTGAAATTGGTTCATGTTAAGCCTCTGGACCGAGAGATTTGTGGGCGTATATTTGAGGAGATTTACAGGGCTAGAGTGGGGAAGCTGAGTGAAGAAGTTACGAGAAAGATTGAGGACATCAAGGATCGGTGCCACGGTCTACCGTTGGTCGCCAAGGCAATCGCAGACACTCTTGATCCTGTGGAAGACTATTATCAGTCTGACTTGTTATCCGCTGTACCCTTTTTCAGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

391

Amino Acids

44.22

Weight (kDa)

6.78

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 153 - 333 1.2e-20 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1068
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 1078
AccIII TCCGGA 1 cut(s) 419
AciI CCGC 5 cut(s) 190, 371, 769, 855, 1152
AclWI GGATC 4 cut(s) 411, 424, 1071, 1109
AcoI YGGCCR 3 cut(s) 234, 339, 712
AcsI RAATTY 3 cut(s) 20, 131, 407
AcuI CTGAAG 1 cut(s) 938
AfaI GTAC 2 cut(s) 718, 1158
AfeI AGCGCT 1 cut(s) 845
AfiI CCNNNNNNNGG 1 cut(s) 644
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 7 cut(s) 43, 103, 136, 295, 689, 904, 943
AjnI CCWGG 2 cut(s) 573, 753
AleI CACNNNNGTG 1 cut(s) 259
AluBI AGCT 7 cut(s) 58, 358, 684, 751, 914, 922, 1024
AluI AGCT 7 cut(s) 58, 358, 684, 751, 914, 922, 1024
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 4 cut(s) 411, 424, 1071, 1109
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 419
Aor51HI AGCGCT 1 cut(s) 845
AoxI GGCC 4 cut(s) 234, 339, 712, 739
ApeKI GCWGC 2 cut(s) 634, 852
ApoI RAATTY 3 cut(s) 20, 131, 407
AseI ATTAAT 1 cut(s) 474
Asp700I GAANNNNTTC 1 cut(s) 35
AspLEI GCGC 1 cut(s) 846
AspS9I GGNCC 2 cut(s) 422, 967
AsuII TTCGAA 1 cut(s) 301
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 2 cut(s) 422, 967
BalI TGGCCA 1 cut(s) 341
BamHI GGATCC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 1068
BauI CACGAG 2 cut(s) 122, 935
BbsI GAAGAC 3 cut(s) 91, 241, 1131
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 621, 864
BccI CCATC 2 cut(s) 98, 698
BceAI ACGGC 2 cut(s) 546, 699
BciT130I CCWGG 2 cut(s) 575, 755
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 5 cut(s) 50, 59, 510, 897, 1011
BfoI RGCGCY 1 cut(s) 847
BglII AGATCT 1 cut(s) 118
BisI GCNGC 4 cut(s) 371, 635, 769, 853
BlpI GCTNAGC 1 cut(s) 764
BlsI GCNGC 4 cut(s) 372, 636, 770, 854
Bme1390I CCNGG 2 cut(s) 575, 755
Bme18I GGWCC 2 cut(s) 422, 967
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 422, 967
BmiI GGNNCC 2 cut(s) 418, 1070
BmrFI CCNGG 2 cut(s) 575, 755
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 3 cut(s) 91, 241, 1131
BpmI CTGGAG 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 764
Bpu14I TTCGAA 1 cut(s) 301
BpuEI CTTGAG 1 cut(s) 380
BsaAI YACGTR 1 cut(s) 695
BsaJI CCNNGG 4 cut(s) 392, 573, 1072, 1092
BsaWI WCCGGW 1 cut(s) 419
Bsc4I CCNNNNNNNGG 1 cut(s) 644
Bse1I ACTGG 2 cut(s) 418, 504
Bse3DI GCAATG 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 419
BseBI CCWGG 2 cut(s) 575, 755
BseDI CCNNGG 4 cut(s) 392, 573, 1072, 1092
BseGI GGATG 2 cut(s) 16, 814
BseLI CCNNNNNNNGG 1 cut(s) 644
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 2 cut(s) 207, 1016
BseNI ACTGG 2 cut(s) 418, 504
BseRI GAGGAG 2 cut(s) 740, 1010
BseXI GCAGC 2 cut(s) 621, 864
BseYI CCCAGC 1 cut(s) 922
BshFI GGCC 4 cut(s) 236, 341, 714, 741
BshNI GGYRCC 1 cut(s) 1068
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 644
BsmAI GTCTC 1 cut(s) 51
BsnI GGCC 4 cut(s) 236, 341, 714, 741
BsoBI CYCGRG 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 301
Bsp1286I GDGCHC 1 cut(s) 213
Bsp13I TCCGGA 1 cut(s) 419
Bsp143I GATC 4 cut(s) 118, 416, 1063, 1114
Bsp1720I GCTNAGC 1 cut(s) 764
BspACI CCGC 5 cut(s) 190, 371, 769, 855, 1152
BspANI GGCC 4 cut(s) 236, 341, 714, 741
BspCNI CTCAG 2 cut(s) 206, 1017
BspEI TCCGGA 1 cut(s) 419
BspLI GGNNCC 2 cut(s) 418, 1070
BspPI GGATC 4 cut(s) 411, 424, 1071, 1109
BspT104I TTCGAA 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 1068
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 2 cut(s) 418, 504
BssECI CCNNGG 4 cut(s) 392, 573, 1072, 1092
BssMI GATC 4 cut(s) 118, 416, 1063, 1114
BssSI CACGAG 2 cut(s) 122, 935
BssT1I CCWWGG 1 cut(s) 1092
Bst2BI CACGAG 2 cut(s) 122, 935
Bst2UI CCWGG 2 cut(s) 575, 755
Bst4CI ACNGT 3 cut(s) 630, 1076, 1083
BstBAI YACGTR 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 301
BstC8I GCNNGC 2 cut(s) 339, 773
BstDEI CTNAG 3 cut(s) 193, 764, 1025
BstDSI CCRYGG 1 cut(s) 1072
BstF5I GGATG 2 cut(s) 16, 814
BstH2I RGCGCY 1 cut(s) 847
BstHHI GCGC 1 cut(s) 846
BstKTI GATC 4 cut(s) 121, 419, 1066, 1117
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 4 cut(s) 118, 416, 1063, 1114
BstMWI GCNNNNNNNGC 1 cut(s) 852
BstNI CCWGG 2 cut(s) 575, 755
BstSCI CCNGG 2 cut(s) 573, 753
BstV1I GCAGC 2 cut(s) 621, 864
BstV2I GAAGAC 3 cut(s) 91, 241, 1131
BstX2I RGATCY 2 cut(s) 118, 416
BstYI RGATCY 2 cut(s) 118, 416
BsuRI GGCC 4 cut(s) 236, 341, 714, 741
BtgI CCRYGG 1 cut(s) 1072
BtsCI GGATG 2 cut(s) 16, 814
BtsI GCAGTG 1 cut(s) 520
BtsIMutI CAGTG 2 cut(s) 411, 520
Cac8I GCNNGC 2 cut(s) 339, 773
CfoI GCGC 1 cut(s) 846
Cfr13I GGNCC 2 cut(s) 422, 967
Csp6I GTAC 2 cut(s) 717, 1157
CviAII CATG 2 cut(s) 257, 953
CviQI GTAC 2 cut(s) 717, 1157
DdeI CTNAG 3 cut(s) 193, 764, 1025
DpnI GATC 4 cut(s) 120, 418, 1065, 1116
DpnII GATC 4 cut(s) 118, 416, 1063, 1114
EaeI YGGCCR 3 cut(s) 234, 339, 712
Eco130I CCWWGG 1 cut(s) 1092
Eco47I GGWCC 2 cut(s) 422, 967
Eco47III AGCGCT 1 cut(s) 845
Eco57I CTGAAG 1 cut(s) 938
Eco88I CYCGRG 1 cut(s) 393
EcoRI GAATTC 3 cut(s) 20, 131, 407
EcoRII CCWGG 2 cut(s) 573, 753
EcoT14I CCWWGG 1 cut(s) 1092
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 1092
FaeI CATG 2 cut(s) 260, 956
FalI AAGNNNNNCTT 2 cut(s) 295, 327
FatI CATG 2 cut(s) 256, 952
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 1 cut(s) 1078
Fnu4HI GCNGC 4 cut(s) 371, 635, 769, 853
FokI GGATG 2 cut(s) 23, 821
Fsp4HI GCNGC 4 cut(s) 371, 635, 769, 853
FspBI CTAG 5 cut(s) 50, 59, 510, 897, 1011
GlaI GCGC 1 cut(s) 845
GluI GCNGC 4 cut(s) 371, 635, 769, 853
GsaI CCCAGC 1 cut(s) 926
GsuI CTGGAG 1 cut(s) 576
HaeII RGCGCY 1 cut(s) 847
HaeIII GGCC 4 cut(s) 236, 341, 714, 741
HapII CCGG 1 cut(s) 420
HhaI GCGC 1 cut(s) 846
Hin1II CATG 2 cut(s) 260, 956
Hin6I GCGC 1 cut(s) 844
HinP1I GCGC 1 cut(s) 844
HinfI GANTC 4 cut(s) 62, 68, 246, 500
HpaII CCGG 1 cut(s) 420
Hpy166II GTNNAC 1 cut(s) 1079
Hpy188I TCNGA 8 cut(s) 67, 130, 196, 449, 582, 918, 1141, 1169
Hpy188III TCNNGA 5 cut(s) 40, 395, 420, 965, 1112
Hpy8I GTNNAC 1 cut(s) 1079
HpyAV CCTTC 3 cut(s) 577, 650, 898
HpyCH4III ACNGT 3 cut(s) 630, 1076, 1083
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 4 cut(s) 230, 264, 283, 852
HpyF10VI GCNNNNNNNGC 1 cut(s) 852
HpyF3I CTNAG 3 cut(s) 193, 764, 1025
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 2 cut(s) 260, 956
HspAI GCGC 1 cut(s) 844
Kpn2I TCCGGA 1 cut(s) 419
Kzo9I GATC 4 cut(s) 118, 416, 1063, 1114
LmnI GCTCC 3 cut(s) 355, 756, 841
Lsp1109I GCAGC 2 cut(s) 621, 864
LweI GCATC 1 cut(s) 217
MaeI CTAG 5 cut(s) 50, 59, 510, 897, 1011
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 2 cut(s) 889, 1036
MalI GATC 4 cut(s) 120, 418, 1065, 1116
MbiI CCGCTC 1 cut(s) 192
MboI GATC 4 cut(s) 118, 416, 1063, 1114
MboII GAAGA 7 cut(s) 91, 241, 315, 622, 691, 1043, 1136
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 2 cut(s) 118, 416
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 2 cut(s) 71, 255
MmeI TCCRAC 1 cut(s) 786
MnlI CCTC 8 cut(s) 355, 402, 592, 718, 723, 972, 988, 1045
Mox20I TGGCCA 1 cut(s) 341
Mph1103I ATGCAT 1 cut(s) 232
MroI TCCGGA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 341
MseI TTAA 5 cut(s) 464, 474, 618, 831, 957
MslI CAYNNNNRTG 1 cut(s) 259
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 2 cut(s) 855, 1154
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 2 cut(s) 575, 755
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 2 cut(s) 575, 755
MwoI GCNNNNNNNGC 1 cut(s) 852
NdeII GATC 4 cut(s) 118, 416, 1063, 1114
NlaIII CATG 2 cut(s) 260, 956
NlaIV GGNNCC 2 cut(s) 418, 1070
NmeAIII GCCGAG 2 cut(s) 262, 269
NmuCI GTSAC 1 cut(s) 889
NsiI ATGCAT 1 cut(s) 232
NspV TTCGAA 1 cut(s) 301
OliI CACNNNNGTG 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 2 cut(s) 68, 500
PfoI TCCNGGA 1 cut(s) 753
PkrI GCNGC 4 cut(s) 372, 636, 770, 854
PleI GAGTC 2 cut(s) 70, 254
PpsI GAGTC 2 cut(s) 70, 254
Ppu21I YACGTR 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 474
Psp6I CCWGG 2 cut(s) 573, 753
PspFI CCCAGC 1 cut(s) 922
PspGI CCWGG 2 cut(s) 573, 753
PspN4I GGNNCC 2 cut(s) 418, 1070
PspPI GGNCC 2 cut(s) 422, 967
PsuI RGATCY 2 cut(s) 118, 416
RsaI GTAC 2 cut(s) 718, 1158
RsaNI GTAC 2 cut(s) 717, 1157
RseI CAYNNNNRTG 1 cut(s) 259
SaqAI TTAA 5 cut(s) 464, 474, 618, 831, 957
SatI GCNGC 4 cut(s) 371, 635, 769, 853
Sau3AI GATC 4 cut(s) 118, 416, 1063, 1114
Sau96I GGNCC 2 cut(s) 422, 967
SchI GAGTC 2 cut(s) 71, 255
ScrFI CCNGG 2 cut(s) 575, 755
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 1 cut(s) 217
SfuI TTCGAA 1 cut(s) 301
SinI GGWCC 2 cut(s) 422, 967
SmiMI CAYNNNNRTG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
SsiI CCGC 5 cut(s) 190, 371, 769, 855, 1152
SspMI CTAG 5 cut(s) 50, 59, 510, 897, 1011
StyD4I CCNGG 2 cut(s) 573, 753
StyI CCWWGG 1 cut(s) 1092
TaaI ACNGT 3 cut(s) 630, 1076, 1083
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 301, 702
TaqII GACCGA 2 cut(s) 419, 984
TauI GCSGC 2 cut(s) 373, 771
TfiI GAWTC 2 cut(s) 68, 500
Tru1I TTAA 5 cut(s) 464, 474, 618, 831, 957
Tru9I TTAA 5 cut(s) 464, 474, 618, 831, 957
TscAI CASTG 2 cut(s) 418, 520
TseFI GTSAC 1 cut(s) 889
TseI GCWGC 2 cut(s) 634, 852
Tsp45I GTSAC 1 cut(s) 889
TspDTI ATGAA 4 cut(s) 304, 492, 623, 941
TspRI CASTG 2 cut(s) 418, 520
VpaK11BI GGWCC 2 cut(s) 422, 967
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 3 cut(s) 20, 131, 407
XmiI GTMKAC 1 cut(s) 1078
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 5 cut(s) 50, 59, 510, 897, 1011
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.