Rh2DG257400

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000202
Physical Location & Seq
Reverse (-)
27406058 .. 27406840
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG257400.1

Sequence Viewer

Length: 783 bp
ATGCCGGAACCTTATTCCTACTCTGAAACGATTAATGTGCTCGACAAACTCAACGATGCATTGACTGAGTGCCGAATCTTCACACATGAGTGCATGGAGCTTAATAATAAGTTCTCACGCTTCAACATTCGAAGATTTACTTTCATTAAAAATATAAAGCGGCGGCTGACCGTAATAAAAAAGGACCTTGAGAAATTGCGGCAACAAGAAATTGGCAATCTTGAGGCAAAGAAAGGAGTCACCGGACCTAAACTTTCAGCAGAAGTTTTGGGATTTGATAAGCAATCAGAGGAGATTGAGGACTTACTACTTGAAGCAGGGGGTTCCAGTTCCAGCAGTGCTGGTGAATTGAGGGCAATTGGGGTAGTTGGTATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTAGAGAGCAAGAGAGTGAAGCTGAAGTTTAATCATATACTTTGGTTACCCTTTTTGTTGAGTGAAGAAGAACAACAATATAGGAAGTCCAGCTTTGAAACATGTATCCATGATAATAATGGCCATATCATAGAGGCGAGAATTGTTGGCCTTGAAGAGCTCCTGGAAAGGCTAAGCCGCCAGTTATCGGATAAATGCTATTTGATTGTGTTGGATGATGTGTGTCATCGGCACATTAACATTATGGAACGGTTATGCAGCGGATTGCCCAGGCATAATGGTGGTGTTGTCATTGTCACTACTAGATTGAAGGTAGTGGCTCAAAAGCTGGAGAAACAACATAGATTGCAATTGACTTGGCCAGTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

260

Amino Acids

29.7

Weight (kDa)

8.83

Isoelectric Point (pI)

65.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 115 - 250 1.4e-18 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 160, 163, 199, 589, 672
AcoI YGGCCR 2 cut(s) 532, 770
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 1 cut(s) 777
AfiI CCNNNNNNNGG 1 cut(s) 598
AflIII ACRYGT 1 cut(s) 512
AgsI TTSAA 5 cut(s) 124, 314, 509, 566, 721
AjnI CCWGG 3 cut(s) 396, 573, 680
AleI CACNNNNGTG 1 cut(s) 88
AluBI AGCT 5 cut(s) 100, 433, 504, 571, 739
AluI AGCT 5 cut(s) 100, 433, 504, 571, 739
Alw21I GWGCWC 2 cut(s) 42, 573
AoxI GGCC 3 cut(s) 532, 559, 770
ApeKI GCWGC 1 cut(s) 669
AseI ATTAAT 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 184, 245
AsuHPI GGTGA 2 cut(s) 232, 356
AsuII TTCGAA 1 cut(s) 130
AvaII GGWCC 2 cut(s) 184, 245
BalI TGGCCA 2 cut(s) 534, 772
BanII GRGCYC 1 cut(s) 573
Bbv12I GWGCWC 2 cut(s) 42, 573
BbvI GCAGC 1 cut(s) 681
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 3 cut(s) 398, 575, 682
BciVI GTATCC 1 cut(s) 527
BfaI CTAG 1 cut(s) 714
BfuI GTATCC 1 cut(s) 527
BisI GCNGC 5 cut(s) 161, 164, 200, 589, 670
BlpI GCTNAGC 1 cut(s) 584
BlsI GCNGC 5 cut(s) 162, 165, 201, 590, 671
Bme1390I CCNGG 3 cut(s) 398, 575, 682
Bme18I GGWCC 2 cut(s) 184, 245
BmgT120I GGNCC 2 cut(s) 184, 245
BmiI GGNNCC 2 cut(s) 9, 325
BmrFI CCNGG 3 cut(s) 398, 575, 682
BmsI GCATC 1 cut(s) 46
BpmI CTGGAG 2 cut(s) 399, 761
Bpu1102I GCTNAGC 1 cut(s) 584
Bpu14I TTCGAA 1 cut(s) 130
BpuEI CTTGAG 2 cut(s) 209, 242
BsaJI CCNNGG 2 cut(s) 396, 680
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse1I ACTGG 3 cut(s) 327, 592, 773
BseBI CCWGG 3 cut(s) 398, 575, 682
BseDI CCNNGG 2 cut(s) 396, 680
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 1 cut(s) 598
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 3 cut(s) 327, 592, 773
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 681
BshFI GGCC 3 cut(s) 534, 561, 772
BsiHKAI GWGCWC 2 cut(s) 42, 573
BsiSI CCGG 2 cut(s) 5, 243
BslI CCNNNNNNNGG 1 cut(s) 598
BsnI GGCC 3 cut(s) 534, 561, 772
Bsp119I TTCGAA 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 42, 573
Bsp1720I GCTNAGC 1 cut(s) 584
BspACI CCGC 5 cut(s) 160, 163, 199, 589, 672
BspANI GGCC 3 cut(s) 534, 561, 772
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 9, 325
BspQI GCTCTTC 1 cut(s) 561
BspT104I TTCGAA 1 cut(s) 130
BsrI ACTGG 3 cut(s) 327, 592, 773
BssECI CCNNGG 2 cut(s) 396, 680
Bst2UI CCWGG 3 cut(s) 398, 575, 682
Bst4CI ACNGT 2 cut(s) 172, 663
Bst6I CTCTTC 1 cut(s) 561
BstBI TTCGAA 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 66, 584
BstEII GGTNACC 1 cut(s) 456
BstF5I GGATG 1 cut(s) 631
BstNI CCWGG 3 cut(s) 398, 575, 682
BstNSI RCATGY 1 cut(s) 516
BstPI GGTNACC 1 cut(s) 456
BstSCI CCNGG 3 cut(s) 396, 573, 680
BstV1I GCAGC 1 cut(s) 681
BsuI GTATCC 1 cut(s) 527
BsuRI GGCC 3 cut(s) 534, 561, 772
BtsCI GGATG 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 184, 245
Csp6I GTAC 1 cut(s) 776
CviAII CATG 4 cut(s) 86, 94, 513, 521
CviQI GTAC 1 cut(s) 776
DdeI CTNAG 2 cut(s) 66, 584
EaeI YGGCCR 2 cut(s) 532, 770
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Ecl136II GAGCTC 1 cut(s) 571
Eco24I GRGCYC 1 cut(s) 573
Eco47I GGWCC 2 cut(s) 184, 245
Eco53kI GAGCTC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 455
Eco91I GGTNACC 1 cut(s) 456
EcoICRI GAGCTC 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 184
EcoO65I GGTNACC 1 cut(s) 456
EcoRII CCWGG 3 cut(s) 396, 573, 680
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 573
FaeI CATG 4 cut(s) 89, 97, 516, 524
FalI AAGNNNNNCTT 4 cut(s) 124, 156, 488, 520
FatI CATG 4 cut(s) 85, 93, 512, 520
Fnu4HI GCNGC 5 cut(s) 161, 164, 200, 589, 670
FokI GGATG 1 cut(s) 638
FriOI GRGCYC 1 cut(s) 573
Fsp4HI GCNGC 5 cut(s) 161, 164, 200, 589, 670
FspBI CTAG 1 cut(s) 714
GluI GCNGC 5 cut(s) 161, 164, 200, 589, 670
GsuI CTGGAG 2 cut(s) 399, 761
HaeIII GGCC 3 cut(s) 534, 561, 772
HapII CCGG 2 cut(s) 5, 243
Hin1II CATG 4 cut(s) 89, 97, 516, 524
HinfI GANTC 2 cut(s) 75, 237
HpaII CCGG 2 cut(s) 5, 243
HphI GGTGA 2 cut(s) 232, 356
Hpy188I TCNGA 4 cut(s) 25, 289, 405, 601
Hpy188III TCNNGA 1 cut(s) 221
HpyAV CCTTC 2 cut(s) 400, 715
HpyCH4III ACNGT 2 cut(s) 172, 663
HpyCH4V TGCA 4 cut(s) 59, 93, 669, 760
HpyF3I CTNAG 2 cut(s) 66, 584
Hsp92II CATG 4 cut(s) 89, 97, 516, 524
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 97, 576
Lsp1109I GCAGC 1 cut(s) 681
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 714
MaeIII GTNAC 3 cut(s) 238, 456, 706
MboII GAAGA 5 cut(s) 70, 144, 488, 491, 578
MfeI CAATTG 2 cut(s) 357, 761
MhlI GDGCHC 2 cut(s) 42, 573
MlsI TGGCCA 2 cut(s) 534, 772
MluCI AATT 6 cut(s) 194, 210, 347, 357, 552, 761
MluNI TGGCCA 2 cut(s) 534, 772
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 603
MnlI CCTC 5 cut(s) 217, 283, 292, 345, 538
Mox20I TGGCCA 2 cut(s) 534, 772
Mph1103I ATGCAT 1 cut(s) 61
MscI TGGCCA 2 cut(s) 534, 772
MseI TTAA 5 cut(s) 33, 102, 147, 441, 648
MslI CAYNNNNRTG 2 cut(s) 88, 690
Msp20I TGGCCA 2 cut(s) 534, 772
MspA1I CMGCKG 1 cut(s) 672
MspI CCGG 2 cut(s) 5, 243
MspR9I CCNGG 3 cut(s) 398, 575, 682
MunI CAATTG 2 cut(s) 357, 761
MvaI CCWGG 3 cut(s) 398, 575, 682
NlaIII CATG 4 cut(s) 89, 97, 516, 524
NlaIV GGNNCC 2 cut(s) 9, 325
NmuCI GTSAC 2 cut(s) 238, 706
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 516
NspV TTCGAA 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 88
PciI ACATGT 1 cut(s) 512
PciSI GCTCTTC 1 cut(s) 561
PfeI GAWTC 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 573
PkrI GCNGC 5 cut(s) 162, 165, 201, 590, 671
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 1 cut(s) 512
PshBI ATTAAT 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 573
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 3 cut(s) 396, 573, 680
PspEI GGTNACC 1 cut(s) 456
PspGI CCWGG 3 cut(s) 396, 573, 680
PspN4I GGNNCC 2 cut(s) 9, 325
PspPI GGNCC 2 cut(s) 184, 245
PspPPI RGGWCCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 777
RsaNI GTAC 1 cut(s) 776
RseI CAYNNNNRTG 2 cut(s) 88, 690
SacI GAGCTC 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 5 cut(s) 33, 102, 147, 441, 648
SatI GCNGC 5 cut(s) 161, 164, 200, 589, 670
Sau96I GGNCC 2 cut(s) 184, 245
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 3 cut(s) 398, 575, 682
SduI GDGCHC 2 cut(s) 42, 573
SfaNI GCATC 1 cut(s) 46
SfuI TTCGAA 1 cut(s) 130
SinI GGWCC 2 cut(s) 184, 245
SmiMI CAYNNNNRTG 2 cut(s) 88, 690
SmlI CTYRAG 2 cut(s) 188, 221
SmoI CTYRAG 2 cut(s) 188, 221
Sse9I AATT 6 cut(s) 194, 210, 347, 357, 552, 761
SsiI CCGC 5 cut(s) 160, 163, 199, 589, 672
SspMI CTAG 1 cut(s) 714
SstI GAGCTC 1 cut(s) 573
StyD4I CCNGG 3 cut(s) 396, 573, 680
TaaI ACNGT 2 cut(s) 172, 663
TaqI TCGA 2 cut(s) 42, 130
TasI AATT 6 cut(s) 194, 210, 347, 357, 552, 761
TauI GCSGC 4 cut(s) 163, 166, 202, 591
TfiI GAWTC 1 cut(s) 75
Tru1I TTAA 5 cut(s) 33, 102, 147, 441, 648
Tru9I TTAA 5 cut(s) 33, 102, 147, 441, 648
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 2 cut(s) 238, 706
TseI GCWGC 1 cut(s) 669
Tsp45I GTSAC 2 cut(s) 238, 706
TspDTI ATGAA 1 cut(s) 133
TspRI CASTG 1 cut(s) 343
VpaK11BI GGWCC 2 cut(s) 184, 245
VspI ATTAAT 1 cut(s) 33
XceI RCATGY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 527
XspI CTAG 1 cut(s) 714
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.