RchiOBHm_Chr1g0372981

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000202
Physical Location & Seq
Forward (+)
61469106 .. 61472612
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59693

Sequence Viewer

Length: 1359 bp
ATGGTACGAGTCCATTTCCAGAGCTTCAATTCTTGTAATTTTGATTGGAATAGTGCTCAGTTTCATGTCTTGGTGAGACGCATTCTTGATAAGTTAAGGGCCGTAGATAGTGGCAATGGTGCCATTGACAATCTCAATGATGCCATAGTGGAGGAGGAAGGAGATATTTCTGGCCTCGGACTTGAGAGGCTCTTGGCAAGGCTTAACCGGCTTTTATCTGGTAAAGGATATTTGATTGTGTTGGATGATGTGTGGCACATTAGCGAATTTTATTCGGATTTAGGTTGTACATTTCAGGATCGGTTATCTTATGGATTGCCTAAGGGTAGTGGTGGTGCTGTTATTGTCACCACTAGGATACCAGAAGTTGCTGAACACATGGTTGGCAGAAATAACTTGATTACTGTTGAGCCTTTGGACACAGAAAGCTGCTGGCGAATATTTATGGAGACTATCAAGGATAATAAGGAAGTTTTAAACATTTCAACTCATGAAACTTTGGATAAGATTAAAAATGAAATTAAGGACCAATGTTACGGTCTACCATTGGCTGCTAAGGAGCTCGCAGGAATCATTCCCAAACGGATTCGTGAGATAGAGTCCAACCGCTTCTTGAAGGAGATGTACATTCCTGATGAATTGCTTCATGCTGATTTGGAAGTCGAACCTGCTGTCCATATACCAAAATTCCCAGTATTGGTGTTTATTGATATGAAAAATATGAACAATAAACAGCTTGGAGAAAAACTCCTTGACAAATTTCGCTATCATCTTAATAAAAAGCAGGTTTTTGCTGTTTTAGAAGAGAAGTCTGATACGAAGAAAAAAGTAAAGGCACATGACCCTGAGAGGGTGCTAAAGGATGTTTATGGCACTTTAGAAAAGCTTAAGCGTAAAGGATATGGTTTTGCTGATGAAATTCAAAAGACGATGACGGTTATAATTGTTGGTGGTGATTCTGTGGCTAACTGGATTTTGGGAGTTATCTGTGATCTGAAATTGCCGGAGTTACCCTCCATTGCTCCAATTCCGCTGCAAACCATTTCTCTTATTGGTGGTAGCATTTCAAGTTCCTTTGGATGGTTTGAAGATAGAGAAATGCAGTTCATGGTTGCTCCTCTTCATGCAGCAATTATAATGCAATTTCAAGATCAGAAAAGCTGGACCTCCAAAGATCTTGCAGCTGCAGTTGGGGTTCCAATAGATACACTTAATCGGAGGATCAATTTTTGGATAAGCAAGGGAATTCTTGCAGAATTACTTGGGGAAGATACTGATGACCATATGTTTACACTAATGGAAGGGATGATTGGTTCTTGCCTCTGTTTTCTATTCTGCTTCGTCATTTTTTATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

452

Amino Acids

51.2

Weight (kDa)

5.57

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 49 - 155 6e-12 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 941, 1136
Acc36I ACCTGC 2 cut(s) 676, 775
AccB1I GGYRCC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 541
AciI CCGC 2 cut(s) 607, 1031
AclWI GGATC 2 cut(s) 306, 1229
AcsI RAATTY 5 cut(s) 266, 686, 758, 918, 1245
AfaI GTAC 3 cut(s) 6, 289, 626
AfiI CCNNNNNNNGG 3 cut(s) 697, 850, 1080
AflII CTTAAG 1 cut(s) 887
AgsI TTSAA 7 cut(s) 28, 486, 616, 923, 1068, 1088, 1148
AjuI GAANNNNNNNTTGG 4 cut(s) 366, 398, 1293, 1325
AloI GAACNNNNNNTCC 4 cut(s) 657, 689, 1297, 1329
AluBI AGCT 7 cut(s) 24, 429, 562, 736, 886, 1161, 1184
AluI AGCT 7 cut(s) 24, 429, 562, 736, 886, 1161, 1184
Alw21I GWGCWC 2 cut(s) 58, 564
Alw26I GTCTC 2 cut(s) 70, 443
AlwI GGATC 2 cut(s) 306, 1229
AoxI GGCC 2 cut(s) 99, 172
ApeKI GCWGC 6 cut(s) 429, 551, 1033, 1127, 1181, 1184
ApoI RAATTY 5 cut(s) 266, 686, 758, 918, 1245
ArsI GACNNNNNNTTYG 2 cut(s) 657, 689
Asp700I GAANNNNTTC 1 cut(s) 642
AspS9I GGNCC 3 cut(s) 99, 526, 1164
AsuHPI GGTGA 3 cut(s) 85, 340, 965
AvaII GGWCC 2 cut(s) 526, 1164
AxyI CCTNAGG 1 cut(s) 321
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 564
BarI GAAGNNNNNNTAC 2 cut(s) 608, 640
Bbv12I GWGCWC 2 cut(s) 58, 564
BbvI GCAGC 6 cut(s) 416, 538, 1020, 1139, 1171, 1193
BccI CCATC 1 cut(s) 1074
BceAI ACGGC 1 cut(s) 86
BciVI GTATCC 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 70, 443
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 1185
BfrI CTTAAG 1 cut(s) 887
BfuAI ACCTGC 2 cut(s) 676, 775
BfuI GTATCC 1 cut(s) 351
BglII AGATCT 1 cut(s) 1174
BisI GCNGC 6 cut(s) 430, 552, 1034, 1128, 1182, 1185
BlsI GCNGC 6 cut(s) 431, 553, 1035, 1129, 1183, 1186
Bme18I GGWCC 2 cut(s) 526, 1164
BmgT120I GGNCC 3 cut(s) 99, 526, 1164
BmiI GGNNCC 2 cut(s) 121, 1197
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 686
BplI GAGNNNNNCTC 4 cut(s) 732, 764, 998, 1030
Bpu10I CCTNAGC 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 3 cut(s) 697, 850, 1080
Bse118I RCCGGY 1 cut(s) 207
Bse1I ACTGG 2 cut(s) 692, 974
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 2 cut(s) 121, 1017
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 4 cut(s) 250, 868, 1085, 1311
BseLI CCNNNNNNNGG 3 cut(s) 697, 850, 1080
BseMI GCAATG 2 cut(s) 121, 1017
BseMII CTCAG 2 cut(s) 71, 837
BseNI ACTGG 2 cut(s) 692, 974
BseRI GAGGAG 2 cut(s) 167, 1107
BseXI GCAGC 6 cut(s) 416, 538, 1020, 1139, 1171, 1193
BshFI GGCC 2 cut(s) 101, 174
BshNI GGYRCC 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 58, 564
BsiSI CCGG 2 cut(s) 208, 1004
BslI CCNNNNNNNGG 3 cut(s) 697, 850, 1080
BsmAI GTCTC 2 cut(s) 70, 443
BsmBI CGTCTC 1 cut(s) 70
BsmI GAATGC 1 cut(s) 81
BsnI GGCC 2 cut(s) 101, 174
Bsp1286I GDGCHC 2 cut(s) 58, 564
Bsp1407I TGTACA 2 cut(s) 287, 624
Bsp143I GATC 5 cut(s) 298, 991, 1150, 1174, 1221
BspACI CCGC 2 cut(s) 607, 1031
BspANI GGCC 2 cut(s) 101, 174
BspCNI CTCAG 2 cut(s) 70, 838
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 121, 1197
BspMAI CTGCAG 1 cut(s) 1189
BspMI ACCTGC 2 cut(s) 676, 775
BspPI GGATC 2 cut(s) 306, 1229
BspT107I GGYRCC 1 cut(s) 119
BspTI CTTAAG 1 cut(s) 887
BsrDI GCAATG 2 cut(s) 121, 1017
BsrFI RCCGGY 1 cut(s) 207
BsrGI TGTACA 2 cut(s) 287, 624
BsrI ACTGG 2 cut(s) 692, 974
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 5 cut(s) 298, 991, 1150, 1174, 1221
Bst4CI ACNGT 3 cut(s) 406, 539, 937
Bst6I CTCTTC 2 cut(s) 798, 1125
BstAFI CTTAAG 1 cut(s) 887
BstAUI TGTACA 2 cut(s) 287, 624
BstC8I GCNNGC 2 cut(s) 434, 564
BstDEI CTNAG 4 cut(s) 57, 321, 555, 846
BstF5I GGATG 4 cut(s) 250, 868, 1085, 1311
BstKTI GATC 5 cut(s) 301, 994, 1153, 1177, 1224
BstMAI GTCTC 2 cut(s) 70, 443
BstMBI GATC 5 cut(s) 298, 991, 1150, 1174, 1221
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstSFI CTRYAG 1 cut(s) 1185
BstV1I GCAGC 6 cut(s) 416, 538, 1020, 1139, 1171, 1193
BstX2I RGATCY 1 cut(s) 1174
BstYI RGATCY 1 cut(s) 1174
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 2 cut(s) 101, 174
BtsCI GGATG 4 cut(s) 250, 868, 1085, 1311
BveI ACCTGC 2 cut(s) 676, 775
Cac8I GCNNGC 2 cut(s) 434, 564
CciI TCATGA 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 3 cut(s) 99, 526, 1164
CseI GACGC 1 cut(s) 87
Csp6I GTAC 3 cut(s) 5, 288, 625
CviAII CATG 7 cut(s) 65, 379, 491, 647, 839, 1108, 1124
CviQI GTAC 3 cut(s) 5, 288, 625
DdeI CTNAG 4 cut(s) 57, 321, 555, 846
DpnI GATC 5 cut(s) 300, 993, 1152, 1176, 1223
DpnII GATC 5 cut(s) 298, 991, 1150, 1174, 1221
DraI TTTAAA 1 cut(s) 477
Eam1104I CTCTTC 2 cut(s) 798, 1125
EarI CTCTTC 2 cut(s) 798, 1125
Ecl136II GAGCTC 1 cut(s) 562
Eco24I GRGCYC 1 cut(s) 564
Eco47I GGWCC 2 cut(s) 526, 1164
Eco53kI GAGCTC 1 cut(s) 562
Eco81I CCTNAGG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 1245
EcoT38I GRGCYC 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 7 cut(s) 68, 382, 494, 650, 842, 1111, 1127
FatI CATG 7 cut(s) 64, 378, 490, 646, 838, 1107, 1123
FauNDI CATATG 1 cut(s) 1284
FblI GTMKAC 1 cut(s) 541
Fnu4HI GCNGC 6 cut(s) 430, 552, 1034, 1128, 1182, 1185
FokI GGATG 4 cut(s) 257, 875, 1092, 1318
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 6 cut(s) 430, 552, 1034, 1128, 1182, 1185
FspBI CTAG 1 cut(s) 354
GluI GCNGC 6 cut(s) 430, 552, 1034, 1128, 1182, 1185
HaeIII GGCC 2 cut(s) 101, 174
HapII CCGG 2 cut(s) 208, 1004
HgaI GACGC 1 cut(s) 87
Hin1II CATG 7 cut(s) 68, 382, 494, 650, 842, 1111, 1127
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 5 cut(s) 9, 570, 586, 599, 956
HpaII CCGG 2 cut(s) 208, 1004
HphI GGTGA 3 cut(s) 85, 340, 965
Hpy166II GTNNAC 2 cut(s) 542, 1290
Hpy188I TCNGA 6 cut(s) 179, 277, 814, 996, 1155, 1218
Hpy188III TCNNGA 8 cut(s) 19, 86, 296, 491, 590, 613, 632, 1148
Hpy8I GTNNAC 2 cut(s) 542, 1290
HpyAV CCTTC 3 cut(s) 152, 610, 1295
HpyCH4III ACNGT 3 cut(s) 406, 539, 937
HpyCH4V TGCA 7 cut(s) 1036, 1102, 1127, 1141, 1181, 1187, 1253
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpyF3I CTNAG 4 cut(s) 57, 321, 555, 846
Hsp92II CATG 7 cut(s) 68, 382, 494, 650, 842, 1111, 1127
Kzo9I GATC 5 cut(s) 298, 991, 1150, 1174, 1221
LmnI GCTCC 3 cut(s) 559, 1027, 1120
Lsp1109I GCAGC 6 cut(s) 416, 538, 1020, 1139, 1171, 1193
LweI GCATC 1 cut(s) 130
MaeI CTAG 1 cut(s) 354
MaeIII GTNAC 3 cut(s) 346, 533, 1008
MalI GATC 5 cut(s) 300, 993, 1152, 1176, 1223
MboI GATC 5 cut(s) 298, 991, 1150, 1174, 1221
MboII GAAGA 5 cut(s) 815, 832, 1100, 1112, 1280
MflI RGATCY 1 cut(s) 1174
MhlI GDGCHC 2 cut(s) 58, 564
MlyI GAGTC 2 cut(s) 18, 608
MmeI TCCRAC 2 cut(s) 222, 627
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 8 cut(s) 95, 204, 476, 510, 522, 774, 888, 1212
MspA1I CMGCKG 2 cut(s) 1033, 1184
MspCI CTTAAG 1 cut(s) 887
MspI CCGG 2 cut(s) 208, 1004
Mva1269I GAATGC 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 208
NdeI CATATG 1 cut(s) 1284
NdeII GATC 5 cut(s) 298, 991, 1150, 1174, 1221
NlaIII CATG 7 cut(s) 68, 382, 494, 650, 842, 1111, 1127
NlaIV GGNNCC 2 cut(s) 121, 1197
NmuCI GTSAC 1 cut(s) 346
PagI TCATGA 1 cut(s) 490
PctI GAATGC 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 570, 586, 956
PkrI GCNGC 6 cut(s) 431, 553, 1035, 1129, 1183, 1186
PleI GAGTC 2 cut(s) 17, 607
PpsI GAGTC 2 cut(s) 17, 607
PsiI TTATAA 2 cut(s) 941, 1136
Psp124BI GAGCTC 1 cut(s) 564
PspN4I GGNNCC 2 cut(s) 121, 1197
PspPI GGNCC 3 cut(s) 99, 526, 1164
PstI CTGCAG 1 cut(s) 1189
PsuI RGATCY 1 cut(s) 1174
PvuII CAGCTG 1 cut(s) 1184
RsaI GTAC 3 cut(s) 6, 289, 626
RsaNI GTAC 3 cut(s) 5, 288, 625
SacI GAGCTC 1 cut(s) 564
SaqAI TTAA 8 cut(s) 95, 204, 476, 510, 522, 774, 888, 1212
SatI GCNGC 6 cut(s) 430, 552, 1034, 1128, 1182, 1185
Sau3AI GATC 5 cut(s) 298, 991, 1150, 1174, 1221
Sau96I GGNCC 3 cut(s) 99, 526, 1164
SchI GAGTC 2 cut(s) 18, 608
SduI GDGCHC 2 cut(s) 58, 564
SfaNI GCATC 1 cut(s) 130
SfcI CTRYAG 1 cut(s) 1185
SinI GGWCC 2 cut(s) 526, 1164
SmlI CTYRAG 2 cut(s) 182, 887
SmoI CTYRAG 2 cut(s) 182, 887
SsiI CCGC 2 cut(s) 607, 1031
SspI AATATT 1 cut(s) 441
SspMI CTAG 1 cut(s) 354
SstI GAGCTC 1 cut(s) 564
TaaI ACNGT 3 cut(s) 406, 539, 937
TaqI TCGA 1 cut(s) 663
TatI WGTACW 2 cut(s) 287, 624
TfiI GAWTC 3 cut(s) 570, 586, 956
Tru1I TTAA 8 cut(s) 95, 204, 476, 510, 522, 774, 888, 1212
Tru9I TTAA 8 cut(s) 95, 204, 476, 510, 522, 774, 888, 1212
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 6 cut(s) 429, 551, 1033, 1127, 1181, 1184
Tsp45I GTSAC 1 cut(s) 346
TspGWI ACGGA 1 cut(s) 598
Vha464I CTTAAG 1 cut(s) 887
VpaK11BI GGWCC 2 cut(s) 526, 1164
XapI RAATTY 5 cut(s) 266, 686, 758, 918, 1245
XmiI GTMKAC 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.