Rorug07G0259400

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000202
Physical Location & Seq
Reverse (-)
24324487 .. 24328915
4429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0259400.1

Sequence Viewer

Length: 2853 bp
ATGCCGATTCTCGAAATACTGTCTGTAGTATTGGGGACTGGCATCACGTCGGCATTTCTGCCTGACGTTGCTAAGAAACTTGTTCAAGTCGGTCGAAAGTTGAGAGCACGAGATCCAGAGATCAATCAAAAGCTCCAAGGGTTGGAAAGAAGATTGTTGAAAATTCAATCCCATGTTGACGATGCCGAAAACAAGGCTTCTATCTCCAATGCCTACCAGATCATAATCGAAGATTTCAAGAATGTTCTTCTGGATGCAGATGATTTGCTGGACAAGACTCTTTTGGAGTATGAGAGGCTGTATTCTCAGAAAGTGAGGCCCGCCAAATTGGAAAAGGATTTGAAATCTTTTGAGGGAAAGCTCGGCAAAGAGATCGGTGAGATGGTGGCTAGGGTGAATGGTTTAGCAAGCGAAATGGAGGGTTACACTATCTCAGAACTAAGCCAACCGAGGCCTGCTCGAACTTCTTCTGCTCCTCCTCAGATTATCAGAGGTTCGTTGGTTGATGATCTCTCTGTAATTGGAAGGGAAAAGGATAAGCAGCAAATCCTGGAGTTGGTGTCTAAGCAGAACAAGACAGCTGTTTCTGTGATTTCAATAGTGGGTATGGTTGGAATTGGGAAGACTACACTTGCCCAGCTAGTCTTCAATGATCAACATCTGGTTGAGGAATTTATGGCCAAAAGGATGTGGTGGGTGAGTGTTTGTTTCGACGATGATGTCCTGAAGATCACCAAATCCATCCTTGAGTCCACTACGAAGACGGTTCCGGATTCCAGTATTGCGCAGTTGTCAAGTGTGGACTCTGTTCAGCTCAAATTAAAGGAGATTGTAGGGGACAAGGAGAAGTTTTTGCTCGTGCTAGATGGCATGTGTGATGAGAATCCTATCAATTGGGATGCCTTGATATTGCCATTTAGATCTGCAGCACCCGGGAGCATAATTGTGGTAACTACTCGAAGCCAAGTTGTTTCATCCATCGTTGCTGATCATGGCAGTACATATTGCTTGGGTGTTTTGTCAGATAAACATTGTTTGGAAATTATCAATCAAGGCTTGAGATCAAGGCTCAGTGAGAAGAGTGGCCTACAGATAGCAAAAAAGTGTAAAGGCCTTCCATTGGTGGCCAAATTGATTGGAAGTGTCATGCGTCTCAAATCAAAAGAGATGGAGTGCGATGAGATCCTAAAATGTGAGCTCTGGAATTTGGCAGAAGATAAGAATCTGATCTATCCAGTGCTTAAACTTGGGTATGATCATTTGCCAGCTTATTTGAAAAGATGTTTTGCTTATTCTTCTCTTCTCCCTCGTAATCGTCCCTTCCAAAGGGATGATTTGATCCAACTGTGGGCAGCTGAAGGCTTTATTGAAGCCCAAGGAACAAGAAGAATCGAAGATATTGGAAGGGAGTACTTTGATGACTTGTGCTCAAGGTCCTTTTTCCAAGTTTCATCAAAGAATCCTGGCGAAGGAAAGTATGAAATGCATGGCTTCATTCATGACTTGGCGAAAATAGTTTCAACCAACTTATGCTTTGCATCTGAGGATACTATGCCACAGGCCTTGCCCATATCTACAAATGTCCGGCATTCCTCAATTTGTGATGAAAAAGTTGAATCAGGTATGTTGGAAGAGTTTTACAAGTATGAGAAGTTAAGGACTTTTATGTTGCTCTCCAAGTACCCATATATGCTTCAAAGTGTTCCTGATGATTTCTTTCGGAAGTTTCCATGCTTAAGAGTGTTAAATTTGAGTGGTACTCGCATCTCTAAATTATCCGGAGATATTAAAATGTTGATACACCTTCGTCACCTCAATGTTAGCGGTACCTTGATTATAAAATTCCCACCGGGTATAGAGCAATTAAATTGGTTAGAAAGACTCAATGTCAAAAATTGTTCCAAACTTGAGGAGCTGCCTGAGAACTTAAACAAGTTGGTTAAGCTACGACATATTGACCTTGACAAACTCGGTAAAGTCACTTCCATGCCTGTTGATGTTGGGAAGTTAACTAGTCTTGAAACCCTGACTGTTTTTGTAGTGGGAAGTGAAGAAGGAAAAAAAATTGAGGAGCTCAAGGACATGAGGTGCCTCCGTGGATCCATTTGCATTCAAAAGCTTGAGAAAGTGGAAGATGACAGAGAAGCTAAGGCTGCTAAGTTACAAGAGAAGCAATACTTGGAGGAATTGGAGTTGCAATGGGAACCAAGGAGATCTGGAACAGTTGACCAAGCAGTTCTTGAGGGTCTTGAACCTCATCAGGGCTTGTTAAAGTTACAAGTAAAAGGTTACTGCGGTTCAAAATTTCCAGATTGGATGAGTAACCCATCGTTTTGCAAGCTTGTGACCATGAATCTAAGAAAATGCGGATCTTGCACACTCCTTCCTTCACTTGGTGGACTTCCTCTTCTCAAACATCTTGTCATTGAAGAAATGAAAAGTTTGAGAACCGTGGATGATCGGTTTTTTCAATCAGATGGTCGGGTATTGCAAAGAGAGGGTTTTCCATCTTTGGTAACGCTAAAATTTGGGGAAATGCCTGCATTGAATACTTGGAAAGGAATGGAAGCATCTGACATGCCTCGCCTTAGAAAGCTTACCATTATAGCTTGTAACGAATTTCGCAACCTACCTTCATTGTACAACCTAGCTGCTCTTAAGGAGTTAGAAATCAATGGTTGCCCTGAGCTTCGATCATTTCCGGGGGAAGGACTACCAAAATCACTCAAAAGGTTGATCATTTCAGAAAGTGACAAATTAAAGCAGAAGTACTGTAGGGTGAATGTGGGTGATTGGGACAAGATCAGATGGGTTAGCCACATAGAGACTGAGGACGGAAGGATTCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

950

Amino Acids

107.4

Weight (kDa)

8.15

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 36 - 100 1.2e-06 Rx N-terminal domain
NB-ARC PF00931 177 - 350 1.1e-23 NB-ARC domain
WHD_DRP PF23559 436 - 503 2.9e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 891 2.8e-33 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 812 5.5e-38 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1838
AasI GACNNNNNNGTC 1 cut(s) 719
Acc16I TGCGCA 1 cut(s) 786
Acc65I GGTACC 1 cut(s) 1826
AccB1I GGYRCC 2 cut(s) 1826, 2088
AccB7I CCANNNNNTGG 1 cut(s) 142
AccIII TCCGGA 2 cut(s) 769, 1778
AciI CCGC 4 cut(s) 321, 1824, 2295, 2367
AclWI GGATC 6 cut(s) 107, 1177, 1333, 2094, 2107, 2377
AcoI YGGCCR 2 cut(s) 678, 1125
AcsI RAATTY 8 cut(s) 162, 671, 1204, 1747, 1841, 2303, 2525, 2618
AcuI CTGAAG 2 cut(s) 746, 1377
AdeI CACNNNGTG 1 cut(s) 2396
AfaI GTAC 7 cut(s) 1000, 1412, 1682, 1759, 1828, 2642, 2771
AfiI CCNNNNNNNGG 9 cut(s) 142, 556, 1120, 1326, 1348, 1469, 2261, 2393, 2708
AflII CTTAAG 2 cut(s) 1735, 2657
AhdI GACNNNNNGTC 1 cut(s) 1886
AhlI ACTAGT 1 cut(s) 2012
AjiI CACGTC 1 cut(s) 48
AjnI CCWGG 2 cut(s) 549, 1462
AjuI GAANNNNNNNTTGG 2 cut(s) 2162, 2194
Alw21I GWGCWC 4 cut(s) 109, 1200, 1430, 2076
Alw26I GTCTC 2 cut(s) 1157, 2819
AlwI GGATC 6 cut(s) 107, 1177, 1333, 2094, 2107, 2377
Ama87I CYCGRG 1 cut(s) 932
Aor13HI TCCGGA 2 cut(s) 769, 1778
AoxI GGCC 7 cut(s) 317, 452, 678, 1084, 1111, 1125, 1560
ApeKI GCWGC 6 cut(s) 541, 926, 1352, 1915, 2153, 2651
ApoI RAATTY 8 cut(s) 162, 671, 1204, 1747, 1841, 2303, 2525, 2618
ArsI GACNNNNNNTTYG 2 cut(s) 2406, 2438
Asp700I GAANNNNTTC 1 cut(s) 466
Asp718I GGTACC 1 cut(s) 1826
AspLEI GCGC 1 cut(s) 787
AspS9I GGNCC 2 cut(s) 318, 1434
AsuC2I CCSGG 4 cut(s) 933, 934, 1851, 2703
AsuHPI GGTGA 7 cut(s) 389, 406, 709, 724, 1802, 2791, 2801
AvaI CYCGRG 1 cut(s) 932
AvaII GGWCC 1 cut(s) 1434
BalI TGGCCA 2 cut(s) 680, 1127
BamHI GGATCC 1 cut(s) 2099
BanI GGYRCC 2 cut(s) 1826, 2088
BanII GRGCYC 2 cut(s) 1200, 2076
BarI GAAGNNNNNNTAC 2 cut(s) 1966, 1998
BauI CACGAG 2 cut(s) 108, 857
BbsI GAAGAC 3 cut(s) 629, 637, 767
Bbv12I GWGCWC 4 cut(s) 109, 1200, 1430, 2076
BbvI GCAGC 6 cut(s) 553, 938, 1364, 1902, 2140, 2638
BccI CCATC 9 cut(s) 376, 749, 860, 986, 1162, 2335, 2471, 2515, 2802
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BciT130I CCWGG 2 cut(s) 551, 1464
BciVI GTATCC 1 cut(s) 1540
BclI TGATCA 4 cut(s) 652, 988, 1255, 2736
BcnI CCSGG 4 cut(s) 933, 934, 1851, 2703
BcoDI GTCTC 2 cut(s) 1157, 2819
BcuI ACTAGT 1 cut(s) 2012
BfaI CTAG 5 cut(s) 390, 641, 863, 2013, 2648
BfmI CTRYAG 4 cut(s) 24, 924, 1088, 2773
BfrI CTTAAG 2 cut(s) 1735, 2657
BfuI GTATCC 1 cut(s) 1540
BglII AGATCT 2 cut(s) 920, 2213
BisI GCNGC 6 cut(s) 542, 927, 1353, 1916, 2154, 2652
BlsI GCNGC 6 cut(s) 543, 928, 1354, 1917, 2155, 2653
BmcAI AGTACT 2 cut(s) 1412, 2771
Bme1390I CCNGG 6 cut(s) 551, 933, 934, 1464, 1851, 2703
Bme18I GGWCC 1 cut(s) 1434
BmeRI GACNNNNNGTC 1 cut(s) 1886
BmeT110I CYCGRG 1 cut(s) 932
BmgBI CACGTC 1 cut(s) 48
BmgT120I GGNCC 2 cut(s) 318, 1434
BmiI GGNNCC 5 cut(s) 768, 1828, 2090, 2101, 2205
BmrFI CCNGG 6 cut(s) 551, 933, 934, 1464, 1851, 2703
BmsI GCATC 7 cut(s) 51, 172, 244, 889, 1547, 1773, 2579
BpiI GAAGAC 3 cut(s) 629, 637, 767
BplI GAGNNNNNCTC 4 cut(s) 442, 474, 1744, 1776
BpmI CTGGAG 1 cut(s) 572
Bpu10I CCTNAGC 2 cut(s) 2148, 2685
BpuEI CTTGAG 7 cut(s) 767, 1078, 1414, 1928, 2060, 2141, 2261
BpuMI CCSGG 4 cut(s) 933, 934, 1851, 2703
BsaBI GATNNNNATC 4 cut(s) 224, 657, 882, 1221
BsaJI CCNNGG 8 cut(s) 136, 449, 932, 1375, 2095, 2207, 2451, 2702
BsaWI WCCGGW 2 cut(s) 769, 1778
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 556, 1120, 1326, 1348, 1469, 2261, 2393, 2708
Bse1I ACTGG 3 cut(s) 43, 777, 1235
Bse3DI GCAATG 1 cut(s) 2204
Bse8I GATNNNNATC 4 cut(s) 224, 657, 882, 1221
BseAI TCCGGA 2 cut(s) 769, 1778
BseBI CCWGG 2 cut(s) 551, 1464
BseDI CCNNGG 8 cut(s) 136, 449, 932, 1375, 2095, 2207, 2451, 2702
BseGI GGATG 8 cut(s) 259, 693, 741, 904, 974, 1336, 2322, 2461
BseJI GATNNNNATC 4 cut(s) 224, 657, 882, 1221
BseLI CCNNNNNNNGG 9 cut(s) 142, 556, 1120, 1326, 1348, 1469, 2261, 2393, 2708
BseMI GCAATG 1 cut(s) 2204
BseMII CTCAG 8 cut(s) 320, 447, 494, 1084, 1533, 1911, 2676, 2820
BseNI ACTGG 3 cut(s) 43, 777, 1235
BseRI GAGGAG 4 cut(s) 465, 468, 1925, 2084
BseXI GCAGC 6 cut(s) 553, 938, 1364, 1902, 2140, 2638
BseYI CCCAGC 1 cut(s) 636
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 7 cut(s) 319, 454, 680, 1086, 1113, 1127, 1562
BshNI GGYRCC 2 cut(s) 1826, 2088
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 4 cut(s) 109, 1200, 1430, 2076
BsiHKCI CYCGRG 1 cut(s) 932
BsiSI CCGG 6 cut(s) 770, 933, 1585, 1779, 1850, 2702
BslFI GGGAC 4 cut(s) 49, 851, 1302, 2810
BslI CCNNNNNNNGG 9 cut(s) 142, 556, 1120, 1326, 1348, 1469, 2261, 2393, 2708
BsmAI GTCTC 2 cut(s) 1157, 2819
BsmBI CGTCTC 1 cut(s) 1157
BsmFI GGGAC 4 cut(s) 49, 851, 1302, 2810
BsmI GAATGC 2 cut(s) 1588, 2109
BsnI GGCC 7 cut(s) 319, 454, 680, 1086, 1113, 1127, 1562
BsoBI CYCGRG 1 cut(s) 932
Bsp1286I GDGCHC 4 cut(s) 109, 1200, 1430, 2076
Bsp13I TCCGGA 2 cut(s) 769, 1778
Bsp1407I TGTACA 1 cut(s) 2640
BspACI CCGC 4 cut(s) 321, 1824, 2295, 2367
BspANI GGCC 7 cut(s) 319, 454, 680, 1086, 1113, 1127, 1562
BspCNI CTCAG 8 cut(s) 319, 446, 493, 1083, 1534, 1912, 2677, 2821
BspEI TCCGGA 2 cut(s) 769, 1778
BspHI TCATGA 1 cut(s) 1498
BspLI GGNNCC 5 cut(s) 768, 1828, 2090, 2101, 2205
BspMAI CTGCAG 1 cut(s) 928
BspPI GGATC 6 cut(s) 107, 1177, 1333, 2094, 2107, 2377
BspT107I GGYRCC 2 cut(s) 1826, 2088
BspTI CTTAAG 2 cut(s) 1735, 2657
BsrDI GCAATG 1 cut(s) 2204
BsrGI TGTACA 1 cut(s) 2640
BsrI ACTGG 3 cut(s) 43, 777, 1235
BssECI CCNNGG 8 cut(s) 136, 449, 932, 1375, 2095, 2207, 2451, 2702
BssSI CACGAG 2 cut(s) 108, 857
BssT1I CCWWGG 3 cut(s) 136, 1375, 2207
Bst2BI CACGAG 2 cut(s) 108, 857
Bst2UI CCWGG 2 cut(s) 551, 1464
Bst4CI ACNGT 8 cut(s) 21, 766, 1347, 2032, 2224, 2452, 2774, 2850
Bst6I CTCTTC 4 cut(s) 1073, 1305, 1626, 2412
BstAFI CTTAAG 2 cut(s) 1735, 2657
BstAUI TGTACA 1 cut(s) 2640
BstC8I GCNNGC 6 cut(s) 321, 409, 456, 1266, 2339, 2541
BstDSI CCRYGG 2 cut(s) 2095, 2451
BstENI CCTNNNNNAGG 2 cut(s) 1324, 1467
BstF5I GGATG 8 cut(s) 259, 693, 741, 904, 974, 1336, 2322, 2461
BstHHI GCGC 1 cut(s) 787
BstMAI GTCTC 2 cut(s) 1157, 2819
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 2 cut(s) 2153, 2373
BstNI CCWGG 2 cut(s) 551, 1464
BstNSI RCATGY 2 cut(s) 874, 2581
BstSCI CCNGG 6 cut(s) 549, 931, 932, 1462, 1849, 2701
BstSFI CTRYAG 4 cut(s) 24, 924, 1088, 2773
BstV1I GCAGC 6 cut(s) 553, 938, 1364, 1902, 2140, 2638
BstV2I GAAGAC 3 cut(s) 629, 637, 767
BstX2I RGATCY 6 cut(s) 112, 920, 1182, 2099, 2213, 2369
BstYI RGATCY 6 cut(s) 112, 920, 1182, 2099, 2213, 2369
BsuI GTATCC 1 cut(s) 1540
BsuRI GGCC 7 cut(s) 319, 454, 680, 1086, 1113, 1127, 1562
BtgI CCRYGG 2 cut(s) 2095, 2451
BtgZI GCGATG 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 8 cut(s) 259, 693, 741, 904, 974, 1336, 2322, 2461
BtsIMutI CAGTG 2 cut(s) 1078, 1242
Cac8I GCNNGC 6 cut(s) 321, 409, 456, 1266, 2339, 2541
CciI TCATGA 1 cut(s) 1498
CfoI GCGC 1 cut(s) 787
Cfr13I GGNCC 2 cut(s) 318, 1434
Cfr9I CCCGGG 1 cut(s) 932
CseI GACGC 1 cut(s) 1139
Csp6I GTAC 7 cut(s) 999, 1411, 1681, 1758, 1827, 2641, 2770
CspCI CAANNNNNGTGG 2 cut(s) 1545, 1580
CviQI GTAC 7 cut(s) 999, 1411, 1681, 1758, 1827, 2641, 2770
DraIII CACNNNGTG 1 cut(s) 2396
DrdI GACNNNNNNGTC 1 cut(s) 719
DriI GACNNNNNGTC 1 cut(s) 1886
DseDI GACNNNNNNGTC 1 cut(s) 719
EaeI YGGCCR 2 cut(s) 678, 1125
Eam1104I CTCTTC 4 cut(s) 1073, 1305, 1626, 2412
Eam1105I GACNNNNNGTC 1 cut(s) 1886
EarI CTCTTC 4 cut(s) 1073, 1305, 1626, 2412
Ecl136II GAGCTC 2 cut(s) 1198, 2074
Eco130I CCWWGG 3 cut(s) 136, 1375, 2207
Eco147I AGGCCT 3 cut(s) 454, 1113, 1562
Eco24I GRGCYC 2 cut(s) 1200, 2076
Eco47I GGWCC 1 cut(s) 1434
Eco53kI GAGCTC 2 cut(s) 1198, 2074
Eco57I CTGAAG 2 cut(s) 746, 1377
Eco88I CYCGRG 1 cut(s) 932
EcoICRI GAGCTC 2 cut(s) 1198, 2074
EcoNI CCTNNNNNAGG 2 cut(s) 1324, 1467
EcoO109I RGGNCCY 1 cut(s) 1434
EcoRII CCWGG 2 cut(s) 549, 1462
EcoT14I CCWWGG 3 cut(s) 136, 1375, 2207
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 2 cut(s) 1200, 2076
ErhI CCWWGG 3 cut(s) 136, 1375, 2207
Esp3I CGTCTC 1 cut(s) 1157
FalI AAGNNNNNCTT 4 cut(s) 2162, 2194, 2223, 2255
FaqI GGGAC 4 cut(s) 49, 851, 1302, 2810
FauI CCCGC 1 cut(s) 328
FbaI TGATCA 4 cut(s) 652, 988, 1255, 2736
Fnu4HI GCNGC 6 cut(s) 542, 927, 1353, 1916, 2154, 2652
FokI GGATG 8 cut(s) 266, 700, 728, 911, 961, 1343, 2329, 2468
FriOI GRGCYC 2 cut(s) 1200, 2076
Fsp4HI GCNGC 6 cut(s) 542, 927, 1353, 1916, 2154, 2652
FspBI CTAG 5 cut(s) 390, 641, 863, 2013, 2648
FspI TGCGCA 1 cut(s) 786
GlaI GCGC 1 cut(s) 786
GluI GCNGC 6 cut(s) 542, 927, 1353, 1916, 2154, 2652
GsaI CCCAGC 1 cut(s) 640
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 7 cut(s) 319, 454, 680, 1086, 1113, 1127, 1562
HapII CCGG 6 cut(s) 770, 933, 1585, 1779, 1850, 2702
HgaI GACGC 1 cut(s) 1139
HhaI GCGC 1 cut(s) 787
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HincII GTYRAC 3 cut(s) 178, 2010, 2227
HindII GTYRAC 3 cut(s) 178, 2010, 2227
HindIII AAGCTT 3 cut(s) 2117, 2339, 2594
HpaI GTTAAC 1 cut(s) 2010
HpaII CCGG 6 cut(s) 770, 933, 1585, 1779, 1850, 2702
HphI GGTGA 7 cut(s) 389, 406, 709, 724, 1802, 2791, 2801
Hpy166II GTNNAC 6 cut(s) 178, 753, 802, 2010, 2227, 2399
Hpy8I GTNNAC 6 cut(s) 178, 753, 802, 2010, 2227, 2399
Hpy99I CGWCG 2 cut(s) 52, 716
HpyCH4III ACNGT 8 cut(s) 21, 766, 1347, 2032, 2224, 2452, 2774, 2850
HpyCH4IV ACGT 2 cut(s) 47, 66
HpyF10VI GCNNNNNNNGC 2 cut(s) 2153, 2373
HpySE526I ACGT 2 cut(s) 47, 66
HspAI GCGC 1 cut(s) 785
Kpn2I TCCGGA 2 cut(s) 769, 1778
KpnI GGTACC 1 cut(s) 1830
Ksp22I TGATCA 4 cut(s) 652, 988, 1255, 2736
KspAI GTTAAC 1 cut(s) 2010
LmnI GCTCC 5 cut(s) 138, 478, 936, 1912, 2071
Lsp1109I GCAGC 6 cut(s) 553, 938, 1364, 1902, 2140, 2638
LweI GCATC 7 cut(s) 51, 172, 244, 889, 1547, 1773, 2579
MaeI CTAG 5 cut(s) 390, 641, 863, 2013, 2648
MaeII ACGT 2 cut(s) 47, 66
MfeI CAATTG 1 cut(s) 892
MflI RGATCY 6 cut(s) 112, 920, 1182, 2099, 2213, 2369
MhlI GDGCHC 4 cut(s) 109, 1200, 1430, 2076
MlsI TGGCCA 2 cut(s) 680, 1127
MluNI TGGCCA 2 cut(s) 680, 1127
MlyI GAGTC 4 cut(s) 271, 758, 797, 1875
MmeI TCCRAC 4 cut(s) 123, 592, 1366, 1608
Mox20I TGGCCA 2 cut(s) 680, 1127
Mph1103I ATGCAT 1 cut(s) 1488
MroI TCCGGA 2 cut(s) 769, 1778
MroXI GAANNNNTTC 1 cut(s) 466
MscI TGGCCA 2 cut(s) 680, 1127
MslI CAYNNNNRTG 3 cut(s) 944, 1815, 1985
Msp20I TGGCCA 2 cut(s) 680, 1127
MspA1I CMGCKG 2 cut(s) 581, 1355
MspCI CTTAAG 2 cut(s) 1735, 2657
MspI CCGG 6 cut(s) 770, 933, 1585, 1779, 1850, 2702
MspR9I CCNGG 6 cut(s) 551, 933, 934, 1464, 1851, 2703
MunI CAATTG 1 cut(s) 892
Mva1269I GAATGC 2 cut(s) 1588, 2109
MvaI CCWGG 2 cut(s) 551, 1464
MwoI GCNNNNNNNGC 2 cut(s) 2153, 2373
NciI CCSGG 4 cut(s) 933, 934, 1851, 2703
NlaIV GGNNCC 5 cut(s) 768, 1828, 2090, 2101, 2205
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 4 cut(s) 1808, 1978, 2344, 2750
NsbI TGCGCA 1 cut(s) 786
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 2 cut(s) 874, 2581
PagI TCATGA 1 cut(s) 1498
PceI AGGCCT 3 cut(s) 454, 1113, 1562
PctI GAATGC 2 cut(s) 1588, 2109
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 9 cut(s) 7, 773, 883, 1222, 1389, 1459, 1616, 2353, 2842
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 1 cut(s) 549
PkrI GCNGC 6 cut(s) 543, 928, 1354, 1917, 2155, 2653
PleI GAGTC 4 cut(s) 271, 757, 797, 1875
PpsI GAGTC 4 cut(s) 271, 757, 797, 1875
PpuMI RGGWCCY 1 cut(s) 1434
PsiI TTATAA 1 cut(s) 1838
Psp124BI GAGCTC 2 cut(s) 1200, 2076
Psp5II RGGWCCY 1 cut(s) 1434
Psp6I CCWGG 2 cut(s) 549, 1462
PspFI CCCAGC 1 cut(s) 636
PspGI CCWGG 2 cut(s) 549, 1462
PspN4I GGNNCC 5 cut(s) 768, 1828, 2090, 2101, 2205
PspPI GGNCC 2 cut(s) 318, 1434
PspPPI RGGWCCY 1 cut(s) 1434
PstI CTGCAG 1 cut(s) 928
PsuI RGATCY 6 cut(s) 112, 920, 1182, 2099, 2213, 2369
PvuII CAGCTG 2 cut(s) 581, 1355
RsaI GTAC 7 cut(s) 1000, 1412, 1682, 1759, 1828, 2642, 2771
RsaNI GTAC 7 cut(s) 999, 1411, 1681, 1758, 1827, 2641, 2770
RseI CAYNNNNRTG 3 cut(s) 944, 1815, 1985
SacI GAGCTC 2 cut(s) 1200, 2076
SatI GCNGC 6 cut(s) 542, 927, 1353, 1916, 2154, 2652
Sau96I GGNCC 2 cut(s) 318, 1434
ScaI AGTACT 2 cut(s) 1412, 2771
SchI GAGTC 4 cut(s) 271, 758, 797, 1875
ScrFI CCNGG 6 cut(s) 551, 933, 934, 1464, 1851, 2703
SduI GDGCHC 4 cut(s) 109, 1200, 1430, 2076
SfaNI GCATC 7 cut(s) 51, 172, 244, 889, 1547, 1773, 2579
SfcI CTRYAG 4 cut(s) 24, 924, 1088, 2773
SinI GGWCC 1 cut(s) 1434
SmaI CCCGGG 1 cut(s) 934
SmiMI CAYNNNNRTG 3 cut(s) 944, 1815, 1985
SmlI CTYRAG 9 cut(s) 746, 1057, 1429, 1735, 1907, 2075, 2120, 2240, 2657
SmoI CTYRAG 9 cut(s) 746, 1057, 1429, 1735, 1907, 2075, 2120, 2240, 2657
SpeI ACTAGT 1 cut(s) 2012
SseBI AGGCCT 3 cut(s) 454, 1113, 1562
SsiI CCGC 4 cut(s) 321, 1824, 2295, 2367
SspMI CTAG 5 cut(s) 390, 641, 863, 2013, 2648
SstI GAGCTC 2 cut(s) 1200, 2076
StuI AGGCCT 3 cut(s) 454, 1113, 1562
StyD4I CCNGG 6 cut(s) 549, 931, 932, 1462, 1849, 2701
StyI CCWWGG 3 cut(s) 136, 1375, 2207
TaaI ACNGT 8 cut(s) 21, 766, 1347, 2032, 2224, 2452, 2774, 2850
TaiI ACGT 2 cut(s) 50, 69
TaqI TCGA 8 cut(s) 12, 94, 228, 460, 711, 958, 1392, 2692
TaqII GACCGA 1 cut(s) 80
TatI WGTACW 4 cut(s) 998, 1410, 2640, 2769
TfiI GAWTC 9 cut(s) 7, 773, 883, 1222, 1389, 1459, 1616, 2353, 2842
TscAI CASTG 2 cut(s) 1078, 1242
TseFI GTSAC 4 cut(s) 1808, 1978, 2344, 2750
TseI GCWGC 6 cut(s) 541, 926, 1352, 1915, 2153, 2651
Tsp45I GTSAC 4 cut(s) 1808, 1978, 2344, 2750
TspDTI ATGAA 9 cut(s) 963, 1440, 1483, 1487, 1494, 1620, 2366, 2450, 2625
TspGWI ACGGA 2 cut(s) 2084, 2850
TspMI CCCGGG 1 cut(s) 932
TspRI CASTG 2 cut(s) 1078, 1242
Van91I CCANNNNNTGG 1 cut(s) 142
Vha464I CTTAAG 2 cut(s) 1735, 2657
VpaK11BI GGWCC 1 cut(s) 1434
XagI CCTNNNNNAGG 2 cut(s) 1324, 1467
XapI RAATTY 8 cut(s) 162, 671, 1204, 1747, 1841, 2303, 2525, 2618
XceI RCATGY 2 cut(s) 874, 2581
XmaI CCCGGG 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 5 cut(s) 390, 641, 863, 2013, 2648
ZrmI AGTACT 2 cut(s) 1412, 2771
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.