Rw0G020260

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00908
OG0000202
Physical Location & Seq
Reverse (-)
64347 .. 69614
5268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020260.1

Sequence Viewer

Length: 2124 bp
ATGTTGATGCCATGGAATGCATCATGGGAAGTGAATACTTATGTTCGGGCAAATACTTATCTCTTGGAGCTCAACTATGCACTGGCAGAGTGCCAAATCTTTGCAAAGGAGTGCAGGGAGCTTAAAACGAAGATTTTACCGTTGAATCCTTCCGCTGTTTTTTTCATCCTACAGATGAAGAAGAGGCTGACGGCACTGAGAAGGAAGTTTGAGACTTTGGTGCTTGAGAGCCCATCAATATCTACTTCTATCTTGAGAGAGGGAAAAGGGAGAAACTCTGTGGAATGTTTGGGATTTACTGAGAAGGATATAGTGGTTTTAAAGAAGAATTCAGAAGAAACTGTGGTGTTTTCTGATGCAATGAATTCAGAAGACTTTACAACTATGGTTGAGCTCTTGGGCTTGCTTGGTGGCTCTGCAGCTGGAATTGGATTTGCTTCCATTGGGATTGTCGGCATGGCTGGCTTGGGTAAGTCCATTTTAGTACAAAGACTTGTGGAAGAGACAGCTGTGCTCTCTGAGTTTTCTCCTATCATTCAGTTATGCTTGTCTGATATAATACAAGAACATAATGTTGTAGACAACACTAATAGTGCCAGTGGGGCCAGCATTAGCATCAGTATTGTGAAACTTATACTTGTAAAGTTGGGGGAGGAGGAGACTGCCGGTATTTCTGGCCTTGAACTTAATTCGCTCTTGGAAAGGCTCAACCACCTTTTATCGGATAAAAAGTATTTAATTGTGTTGGATGATGTGTGGCATAATATGGAGTTCTATAGGGACCTTGGCTATCAGCTTCCTGATGGTGAGAAATTTGGGGATTGGTTATCTGATGGGTTGCCTAAGGATGATTTATCTTATAAGTTACCCGAAGGTGAGAAAATTGGGGTTCAGTTATCTCACGGATTGCCCAAGGACTGTGATGGTACGGTCATTGTCACTAGTAGGATAACTGAAGTGGCTCAACATATGGTTGGCTTGGGCCAACATAACTTGGTTCTTGTTACGCCTTGGGACAGAGGACAGTGCTTGTTATACTTCGACAGCTTTATCTTCCATGAAAGATTACGGTGTGGTAAAGAAAAACGTTCTGGCGTGTCAAATACAGAAACTGTGAGAAAGGTCCATAATGAAATCCTGGATCAAGTTTGTGGTCTGCCATTAATGGTCAAAACTCTCGCATACTTGATCTTTGAAAACCAGATTAGTCAGAATGATTATAGTAGGTCTGCCCTGAAGAAGATGTTGATACCTCGTGAATTTCTTACTGATTGTTCATGGGAGTATGTTCCGATCAACGAAAGAAATTTCGACTGGGCTCATGATAAAATGGACATGCCGAAATGCCCAGTTTTTGTGTTTATTGATTTCCAAAGCGACCTTATAGGAGCTCTAGCTGAAGACTTTCGCTACCAGCTTAATCGTGTGCAGGTTCTTTCTGTGTTACATGAGAGTTTCATTACGCGAAGATGTTCGCCAATGAAACCAGAAAGGGCGTTAGAGGAGTTTTATGGTTCTCTAGCAAAGTTTAAGGGTGAAGGACATGATTTTGCTGATGAAATTCGAAAGAAGATGACATTTATAATTGTTGGTGGAGATGTTTTTGTTAACGGGTTTTTAGGAGTTTTATGCGATCTAAAATTGCCAGACTCACCCTCCATTGTTCCCATTGCACTCGGAATCAAAAGTAACATTCCATTTTTGTTTGGATGGGAATTTGATGCAATGAAACCAGTGAACAAGATATTGGAGACAGTTCTAAAGCATACAAAACATATCAAAACAGATAGCTGGCATATCCTCATCAAGATGGAAGCCGCCATTCCTACAGATTATCAAGAAATACCATATTGTTTGCAGTTATGTCATCCAGTTCACAAACCAAATATGGAAAATGTGGATAACCAGCCATTAATCCGTGGAGAATTTTGGAACTACTTCAGCTTTGGTGTTGACGATCCAAGATTTTCTGTATGTGCATGTAAAGTTGTTTCGGTGATTGGCAATTGCAATCAGATAGACTTGACCCCATCTTTGATAGATGATGGCCGTCTTGAAATTATTGGTTCCAGAGATGATTTGCTTTGTCCGAAGAAAAGGTGGATTCGCCTTGATCAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

707

Amino Acids

79.93

Weight (kDa)

5.48

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 147 - 270 2e-11 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 861, 1583
Acc36I ACCTGC 1 cut(s) 1420
AccI GTMKAC 1 cut(s) 579
AccII CGCG 1 cut(s) 1465
AciI CCGC 2 cut(s) 153, 1818
AclI AACGTT 1 cut(s) 1087
AclWI GGATC 2 cut(s) 1149, 1952
AcoI YGGCCR 1 cut(s) 2047
AcsI RAATTY 8 cut(s) 328, 364, 812, 1259, 1306, 1560, 1715, 1925
AcuI CTGAAG 4 cut(s) 975, 1256, 1419, 1924
AfaI GTAC 2 cut(s) 486, 928
AfiI CCNNNNNNNGG 2 cut(s) 721, 2116
AgsI TTSAA 4 cut(s) 145, 683, 1196, 2057
AhlI ACTAGT 1 cut(s) 941
AjnI CCWGG 1 cut(s) 1137
AjuI GAANNNNNNNTTGG 2 cut(s) 1730, 1762
Alw21I GWGCWC 4 cut(s) 72, 396, 516, 1393
Alw26I GTCTC 4 cut(s) 206, 497, 653, 1745
AlwI GGATC 2 cut(s) 1149, 1952
AlwNI CAGNNNCTG 1 cut(s) 1112
AoxI GGCC 4 cut(s) 603, 676, 982, 2047
ApeKI GCWGC 1 cut(s) 419
ApoI RAATTY 8 cut(s) 328, 364, 812, 1259, 1306, 1560, 1715, 1925
ArsI GACNNNNNNTTYG 2 cut(s) 2017, 2049
AseI ATTAAT 2 cut(s) 1163, 1913
Asp700I GAANNNNTTC 3 cut(s) 1404, 1471, 1937
AspS9I GGNCC 4 cut(s) 603, 781, 982, 1123
AsuHPI GGTGA 5 cut(s) 818, 887, 1547, 1644, 2008
AsuII TTCGAA 1 cut(s) 1564
AvaII GGWCC 2 cut(s) 781, 1123
AxyI CCTNAGG 1 cut(s) 843
BanII GRGCYC 5 cut(s) 72, 233, 396, 1321, 1393
BauI CACGAG 1 cut(s) 1254
BbsI GAAGAC 2 cut(s) 378, 1407
Bbv12I GWGCWC 4 cut(s) 72, 396, 516, 1393
BbvI GCAGC 1 cut(s) 431
BccI CCATC 8 cut(s) 241, 797, 827, 917, 1704, 1804, 2038, 2039
BceAI ACGGC 2 cut(s) 207, 2034
BciT130I CCWGG 1 cut(s) 1139
BclI TGATCA 1 cut(s) 2113
BcoDI GTCTC 4 cut(s) 206, 497, 653, 1745
BcuI ACTAGT 1 cut(s) 941
BfaI CTAG 3 cut(s) 942, 1394, 1520
BfmI CTRYAG 4 cut(s) 170, 417, 775, 1827
BfuAI ACCTGC 1 cut(s) 1420
BglI GCCNNNNNGGC 1 cut(s) 602
BisI GCNGC 2 cut(s) 420, 1818
BlsI GCNGC 2 cut(s) 421, 1819
Bme1390I CCNGG 1 cut(s) 1139
Bme18I GGWCC 2 cut(s) 781, 1123
BmgT120I GGNCC 4 cut(s) 603, 781, 982, 1123
BmiI GGNNCC 3 cut(s) 604, 782, 2068
BmrFI CCNGG 1 cut(s) 1139
BmrI ACTGGG 2 cut(s) 1324, 1343
BmsI GCATC 4 cut(s) 29, 346, 624, 1711
BmuI ACTGGG 2 cut(s) 1324, 1343
BpiI GAAGAC 2 cut(s) 378, 1407
Bpu14I TTCGAA 1 cut(s) 1564
BpuEI CTTGAG 2 cut(s) 245, 274
BsaJI CCNNGG 5 cut(s) 11, 784, 912, 1010, 1918
BsaXI ACNNNNNCTCC 2 cut(s) 1640, 1670
Bsc4I CCNNNNNNNGG 2 cut(s) 721, 2116
Bse118I RCCGGY 1 cut(s) 665
Bse1I ACTGG 6 cut(s) 87, 597, 1319, 1349, 1733, 1871
Bse21I CCTNAGG 1 cut(s) 843
Bse3DI GCAATG 3 cut(s) 366, 1668, 1731
BseBI CCWGG 1 cut(s) 1139
BseDI CCNNGG 5 cut(s) 11, 784, 912, 1010, 1918
BseGI GGATG 5 cut(s) 165, 754, 853, 1715, 1867
BseLI CCNNNNNNNGG 2 cut(s) 721, 2116
BseMI GCAATG 3 cut(s) 366, 1668, 1731
BseMII CTCAG 3 cut(s) 188, 291, 510
BseNI ACTGG 6 cut(s) 87, 597, 1319, 1349, 1733, 1871
BseRI GAGGAG 3 cut(s) 668, 671, 1517
BseXI GCAGC 1 cut(s) 431
BsgI GTGCAG 2 cut(s) 133, 1448
Bsh1236I CGCG 1 cut(s) 1465
BshFI GGCC 4 cut(s) 605, 678, 984, 2049
BsiHKAI GWGCWC 4 cut(s) 72, 396, 516, 1393
BsiSI CCGG 1 cut(s) 666
BslFI GGGAC 2 cut(s) 794, 1028
BslI CCNNNNNNNGG 2 cut(s) 721, 2116
BsmAI GTCTC 4 cut(s) 206, 497, 653, 1745
BsmFI GGGAC 2 cut(s) 794, 1028
BsmI GAATGC 1 cut(s) 22
BsnI GGCC 4 cut(s) 605, 678, 984, 2049
Bsp119I TTCGAA 1 cut(s) 1564
Bsp1286I GDGCHC 6 cut(s) 72, 233, 396, 516, 1321, 1393
Bsp143I GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
Bsp19I CCATGG 1 cut(s) 11
BspACI CCGC 2 cut(s) 153, 1818
BspANI GGCC 4 cut(s) 605, 678, 984, 2049
BspCNI CTCAG 3 cut(s) 189, 292, 511
BspFNI CGCG 1 cut(s) 1465
BspHI TCATGA 1 cut(s) 1321
BspLI GGNNCC 3 cut(s) 604, 782, 2068
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 1420
BspPI GGATC 2 cut(s) 1149, 1952
BspT104I TTCGAA 1 cut(s) 1564
BsrDI GCAATG 3 cut(s) 366, 1668, 1731
BsrFI RCCGGY 1 cut(s) 665
BsrI ACTGG 6 cut(s) 87, 597, 1319, 1349, 1733, 1871
BssAI RCCGGY 1 cut(s) 665
BssECI CCNNGG 5 cut(s) 11, 784, 912, 1010, 1918
BssMI GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
BssSI CACGAG 1 cut(s) 1254
BssT1I CCWWGG 4 cut(s) 11, 784, 912, 1010
Bst2BI CACGAG 1 cut(s) 1254
Bst2UI CCWGG 1 cut(s) 1139
Bst4CI ACNGT 8 cut(s) 141, 343, 920, 931, 1026, 1071, 1114, 1756
Bst6I CTCTTC 2 cut(s) 176, 495
BstBI TTCGAA 1 cut(s) 1564
BstC8I GCNNGC 4 cut(s) 404, 463, 607, 1793
BstDEI CTNAG 4 cut(s) 197, 300, 519, 843
BstDSI CCRYGG 2 cut(s) 11, 1918
BstENI CCTNNNNNAGG 1 cut(s) 2114
BstF5I GGATG 5 cut(s) 165, 754, 853, 1715, 1867
BstFNI CGCG 1 cut(s) 1465
BstKTI GATC 6 cut(s) 1144, 1191, 1296, 1636, 1960, 2116
BstMAI GTCTC 4 cut(s) 206, 497, 653, 1745
BstMBI GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
BstMWI GCNNNNNNNGC 2 cut(s) 462, 602
BstNI CCWGG 1 cut(s) 1139
BstNSI RCATGY 2 cut(s) 1339, 1983
BstSCI CCNGG 1 cut(s) 1137
BstSFI CTRYAG 4 cut(s) 170, 417, 775, 1827
BstUI CGCG 1 cut(s) 1465
BstV1I GCAGC 1 cut(s) 431
BstV2I GAAGAC 2 cut(s) 378, 1407
Bsu36I CCTNAGG 1 cut(s) 843
BsuRI GGCC 4 cut(s) 605, 678, 984, 2049
BtgI CCRYGG 2 cut(s) 11, 1918
BtsCI GGATG 5 cut(s) 165, 754, 853, 1715, 1867
BtsIMutI CAGTG 5 cut(s) 80, 194, 604, 1031, 1740
BveI ACCTGC 1 cut(s) 1420
Cac8I GCNNGC 4 cut(s) 404, 463, 607, 1793
CaiI CAGNNNCTG 1 cut(s) 1112
CciI TCATGA 1 cut(s) 1321
Cfr10I RCCGGY 1 cut(s) 665
Cfr13I GGNCC 4 cut(s) 603, 781, 982, 1123
Csp6I GTAC 2 cut(s) 485, 927
CviQI GTAC 2 cut(s) 485, 927
DdeI CTNAG 4 cut(s) 197, 300, 519, 843
DpnI GATC 6 cut(s) 1143, 1190, 1295, 1635, 1959, 2115
DpnII GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
DraI TTTAAA 1 cut(s) 321
EaeI YGGCCR 1 cut(s) 2047
Eam1104I CTCTTC 2 cut(s) 176, 495
EarI CTCTTC 2 cut(s) 176, 495
Ecl136II GAGCTC 3 cut(s) 70, 394, 1391
Eco130I CCWWGG 4 cut(s) 11, 784, 912, 1010
Eco24I GRGCYC 5 cut(s) 72, 233, 396, 1321, 1393
Eco47I GGWCC 2 cut(s) 781, 1123
Eco53kI GAGCTC 3 cut(s) 70, 394, 1391
Eco57I CTGAAG 4 cut(s) 975, 1256, 1419, 1924
Eco81I CCTNAGG 1 cut(s) 843
EcoICRI GAGCTC 3 cut(s) 70, 394, 1391
EcoNI CCTNNNNNAGG 1 cut(s) 2114
EcoO109I RGGNCCY 1 cut(s) 781
EcoRI GAATTC 2 cut(s) 328, 364
EcoRII CCWGG 1 cut(s) 1137
EcoT14I CCWWGG 4 cut(s) 11, 784, 912, 1010
EcoT22I ATGCAT 1 cut(s) 22
EcoT38I GRGCYC 5 cut(s) 72, 233, 396, 1321, 1393
ErhI CCWWGG 4 cut(s) 11, 784, 912, 1010
FaqI GGGAC 2 cut(s) 794, 1028
FauNDI CATATG 1 cut(s) 969
FbaI TGATCA 1 cut(s) 2113
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 420, 1818
FokI GGATG 5 cut(s) 152, 761, 860, 1722, 1854
FriOI GRGCYC 5 cut(s) 72, 233, 396, 1321, 1393
Fsp4HI GCNGC 2 cut(s) 420, 1818
FspBI CTAG 3 cut(s) 942, 1394, 1520
GluI GCNGC 2 cut(s) 420, 1818
HaeIII GGCC 4 cut(s) 605, 678, 984, 2049
HapII CCGG 1 cut(s) 666
HincII GTYRAC 2 cut(s) 1609, 1954
HindII GTYRAC 2 cut(s) 1609, 1954
HinfI GANTC 4 cut(s) 145, 1649, 1680, 2104
HpaI GTTAAC 1 cut(s) 1609
HpaII CCGG 1 cut(s) 666
HphI GGTGA 5 cut(s) 818, 887, 1547, 1644, 2008
Hpy166II GTNNAC 5 cut(s) 580, 1609, 1738, 1876, 1954
Hpy188III TCNNGA 8 cut(s) 253, 800, 1256, 1322, 1807, 1838, 2054, 2070
Hpy8I GTNNAC 5 cut(s) 580, 1609, 1738, 1876, 1954
HpyAV CCTTC 5 cut(s) 159, 195, 298, 866, 1532
HpyCH4III ACNGT 8 cut(s) 141, 343, 920, 931, 1026, 1071, 1114, 1756
HpyCH4IV ACGT 1 cut(s) 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 462, 602
HpyF3I CTNAG 4 cut(s) 197, 300, 519, 843
HpySE526I ACGT 1 cut(s) 1087
Ksp22I TGATCA 1 cut(s) 2113
KspAI GTTAAC 1 cut(s) 1609
Kzo9I GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
LmnI GCTCC 3 cut(s) 67, 118, 1388
Lsp1109I GCAGC 1 cut(s) 431
LweI GCATC 4 cut(s) 29, 346, 624, 1711
MaeI CTAG 3 cut(s) 942, 1394, 1520
MaeII ACGT 1 cut(s) 1087
MaeIII GTNAC 5 cut(s) 864, 937, 1003, 1443, 1688
MalI GATC 6 cut(s) 1143, 1190, 1295, 1635, 1959, 2115
MboI GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
MfeI CAATTG 1 cut(s) 2005
MhlI GDGCHC 6 cut(s) 72, 233, 396, 516, 1321, 1393
MlyI GAGTC 1 cut(s) 1643
MmeI TCCRAC 1 cut(s) 726
MnlI CCTC 9 cut(s) 177, 253, 646, 649, 1013, 1263, 1495, 1666, 1811
Mph1103I ATGCAT 1 cut(s) 22
MroXI GAANNNNTTC 3 cut(s) 1404, 1471, 1937
MseI TTAA 9 cut(s) 123, 320, 687, 737, 1163, 1419, 1530, 1608, 1913
MslI CAYNNNNRTG 1 cut(s) 1808
MspA1I CMGCKG 3 cut(s) 155, 422, 509
MspI CCGG 1 cut(s) 666
MspR9I CCNGG 1 cut(s) 1139
MunI CAATTG 1 cut(s) 2005
Mva1269I GAATGC 1 cut(s) 22
MvaI CCWGG 1 cut(s) 1139
MvnI CGCG 1 cut(s) 1465
MwoI GCNNNNNNNGC 2 cut(s) 462, 602
NcoI CCATGG 1 cut(s) 11
NdeI CATATG 1 cut(s) 969
NdeII GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
NlaIV GGNNCC 3 cut(s) 604, 782, 2068
NmuCI GTSAC 1 cut(s) 937
NsiI ATGCAT 1 cut(s) 22
NspI RCATGY 2 cut(s) 1339, 1983
NspV TTCGAA 1 cut(s) 1564
PagI TCATGA 1 cut(s) 1321
PctI GAATGC 1 cut(s) 22
PdmI GAANNNNTTC 3 cut(s) 1404, 1471, 1937
PfeI GAWTC 3 cut(s) 145, 1680, 2104
PfoI TCCNGGA 1 cut(s) 1137
PkrI GCNGC 2 cut(s) 421, 1819
PleI GAGTC 1 cut(s) 1643
PpsI GAGTC 1 cut(s) 1643
PpuMI RGGWCCY 1 cut(s) 781
PshBI ATTAAT 2 cut(s) 1163, 1913
PsiI TTATAA 2 cut(s) 861, 1583
Psp124BI GAGCTC 3 cut(s) 72, 396, 1393
Psp1406I AACGTT 1 cut(s) 1087
Psp5II RGGWCCY 1 cut(s) 781
Psp6I CCWGG 1 cut(s) 1137
PspGI CCWGG 1 cut(s) 1137
PspN4I GGNNCC 3 cut(s) 604, 782, 2068
PspPI GGNCC 4 cut(s) 603, 781, 982, 1123
PspPPI RGGWCCY 1 cut(s) 781
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 1112
PvuII CAGCTG 2 cut(s) 422, 509
RsaI GTAC 2 cut(s) 486, 928
RsaNI GTAC 2 cut(s) 485, 927
RseI CAYNNNNRTG 1 cut(s) 1808
SacI GAGCTC 3 cut(s) 72, 396, 1393
SaqAI TTAA 9 cut(s) 123, 320, 687, 737, 1163, 1419, 1530, 1608, 1913
SatI GCNGC 2 cut(s) 420, 1818
Sau3AI GATC 6 cut(s) 1141, 1188, 1293, 1633, 1957, 2113
Sau96I GGNCC 4 cut(s) 603, 781, 982, 1123
SchI GAGTC 1 cut(s) 1643
ScrFI CCNGG 1 cut(s) 1139
SduI GDGCHC 6 cut(s) 72, 233, 396, 516, 1321, 1393
SfaNI GCATC 4 cut(s) 29, 346, 624, 1711
SfcI CTRYAG 4 cut(s) 170, 417, 775, 1827
SfuI TTCGAA 1 cut(s) 1564
SinI GGWCC 2 cut(s) 781, 1123
SmiMI CAYNNNNRTG 1 cut(s) 1808
SmlI CTYRAG 2 cut(s) 224, 253
SmoI CTYRAG 2 cut(s) 224, 253
SpeI ACTAGT 1 cut(s) 941
SsiI CCGC 2 cut(s) 153, 1818
SspMI CTAG 3 cut(s) 942, 1394, 1520
SstI GAGCTC 3 cut(s) 72, 396, 1393
StyD4I CCNGG 1 cut(s) 1137
StyI CCWWGG 4 cut(s) 11, 784, 912, 1010
TaaI ACNGT 8 cut(s) 141, 343, 920, 931, 1026, 1071, 1114, 1756
TaiI ACGT 1 cut(s) 1090
TaqI TCGA 3 cut(s) 1041, 1311, 1564
TatI WGTACW 1 cut(s) 484
TauI GCSGC 1 cut(s) 1820
TfiI GAWTC 3 cut(s) 145, 1680, 2104
Tru1I TTAA 9 cut(s) 123, 320, 687, 737, 1163, 1419, 1530, 1608, 1913
Tru9I TTAA 9 cut(s) 123, 320, 687, 737, 1163, 1419, 1530, 1608, 1913
TscAI CASTG 5 cut(s) 87, 201, 604, 1031, 1740
TseFI GTSAC 1 cut(s) 937
TseI GCWGC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 937
TspGWI ACGGA 2 cut(s) 918, 1907
TspRI CASTG 5 cut(s) 87, 201, 604, 1031, 1740
VpaK11BI GGWCC 2 cut(s) 781, 1123
VspI ATTAAT 2 cut(s) 1163, 1913
XagI CCTNNNNNAGG 1 cut(s) 2114
XapI RAATTY 8 cut(s) 328, 364, 812, 1259, 1306, 1560, 1715, 1925
XceI RCATGY 2 cut(s) 1339, 1983
XmiI GTMKAC 1 cut(s) 579
XmnI GAANNNNTTC 3 cut(s) 1404, 1471, 1937
XspI CTAG 3 cut(s) 942, 1394, 1520
Zsp2I ATGCAT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.