Diacylglycerol kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1568 |
| Acc36I | ACCTGC | 2 cut(s) | 1303, 1402 |
| AccB1I | GGYRCC | 1 cut(s) | 746 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2537 |
| AccBSI | CCGCTC | 2 cut(s) | 2181, 2211 |
| AccI | GTMKAC | 1 cut(s) | 1168 |
| AccII | CGCG | 1 cut(s) | 218 |
| AccIII | TCCGGA | 1 cut(s) | 1916 |
| AclWI | GGATC | 2 cut(s) | 933, 1724 |
| AcoI | YGGCCR | 4 cut(s) | 222, 240, 2275, 2440 |
| AcsI | RAATTY | 6 cut(s) | 671, 893, 1313, 1385, 1545, 2454 |
| AcuI | CTGAAG | 2 cut(s) | 1505, 1921 |
| AfaI | GTAC | 6 cut(s) | 451, 916, 1253, 1758, 2448, 2542 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 238, 1324, 1477, 1707, 2443, 2449, 2537, 2635 |
| AflII | CTTAAG | 1 cut(s) | 1514 |
| AflIII | ACRYGT | 2 cut(s) | 216, 246 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 61 |
| AjnI | CCWGG | 2 cut(s) | 591, 2442 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 993, 1025 |
| AleI | CACNNNNGTG | 1 cut(s) | 584 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1284, 1316 |
| Alw21I | GWGCWC | 3 cut(s) | 210, 591, 1191 |
| Alw26I | GTCTC | 2 cut(s) | 697, 1070 |
| Alw44I | GTGCAC | 1 cut(s) | 587 |
| AlwI | GGATC | 2 cut(s) | 933, 1724 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 65 |
| Ama87I | CYCGRG | 1 cut(s) | 231 |
| Aor13HI | TCCGGA | 1 cut(s) | 1916 |
| AoxI | GGCC | 8 cut(s) | 222, 240, 726, 799, 2102, 2204, 2275, 2440 |
| ApaLI | GTGCAC | 1 cut(s) | 587 |
| ApoI | RAATTY | 6 cut(s) | 671, 893, 1313, 1385, 1545, 2454 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 178, 210, 1284, 1316 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1269, 1934 |
| AspLEI | GCGC | 3 cut(s) | 470, 2134, 2263 |
| AspS9I | GGNCC | 4 cut(s) | 726, 1153, 1998, 2102 |
| AsuC2I | CCSGG | 2 cut(s) | 140, 426 |
| AsuHPI | GGTGA | 7 cut(s) | 431, 967, 1592, 2426, 2489, 2653, 2780 |
| AvaI | CYCGRG | 1 cut(s) | 231 |
| AvaII | GGWCC | 2 cut(s) | 1153, 1998 |
| AxyI | CCTNAGG | 1 cut(s) | 948 |
| BaeGI | GKGCMC | 1 cut(s) | 591 |
| BalI | TGGCCA | 2 cut(s) | 242, 2442 |
| BanI | GGYRCC | 1 cut(s) | 746 |
| BanII | GRGCYC | 2 cut(s) | 210, 1191 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1235, 1267 |
| Bbv12I | GWGCWC | 3 cut(s) | 210, 591, 1191 |
| BccI | CCATC | 5 cut(s) | 1701, 2366, 2468, 2501, 2531 |
| BceAI | ACGGC | 2 cut(s) | 555, 713 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2197, 2231 |
| BciT130I | CCWGG | 2 cut(s) | 593, 2444 |
| BciVI | GTATCC | 2 cut(s) | 978, 2636 |
| BclI | TGATCA | 1 cut(s) | 400 |
| BcnI | CCSGG | 2 cut(s) | 140, 426 |
| BcoDI | GTCTC | 2 cut(s) | 697, 1070 |
| BfaI | CTAG | 7 cut(s) | 413, 584, 981, 1751, 2067, 2139, 2310 |
| BfmI | CTRYAG | 2 cut(s) | 1827, 2094 |
| BfoI | RGCGCY | 1 cut(s) | 2264 |
| BfrI | CTTAAG | 1 cut(s) | 1514 |
| BfuAI | ACCTGC | 2 cut(s) | 1303, 1402 |
| BfuI | GTATCC | 2 cut(s) | 978, 2636 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2212 |
| BlpI | GCTNAGC | 1 cut(s) | 2177 |
| Bme1390I | CCNGG | 4 cut(s) | 140, 426, 593, 2444 |
| Bme18I | GGWCC | 2 cut(s) | 1153, 1998 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 61 |
| BmeT110I | CYCGRG | 1 cut(s) | 231 |
| BmgT120I | GGNCC | 4 cut(s) | 726, 1153, 1998, 2102 |
| BmiI | GGNNCC | 3 cut(s) | 163, 748, 2428 |
| BmrFI | CCNGG | 4 cut(s) | 140, 426, 593, 2444 |
| BmrI | ACTGGG | 1 cut(s) | 1313 |
| BmsI | GCATC | 7 cut(s) | 693, 757, 1698, 1867, 1942, 2289, 2641 |
| BmuI | ACTGGG | 1 cut(s) | 1313 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1359, 1391, 1625, 1657, 1705, 1737 |
| BpmI | CTGGAG | 3 cut(s) | 2138, 2212, 2285 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1182 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2177 |
| BpuEI | CTTGAG | 3 cut(s) | 46, 830, 1678 |
| BpuMI | CCSGG | 2 cut(s) | 140, 426 |
| BsaBI | GATNNNNATC | 2 cut(s) | 149, 2376 |
| BsaJI | CCNNGG | 3 cut(s) | 802, 2437, 2516 |
| BsaWI | WCCGGW | 2 cut(s) | 1882, 1916 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 362, 392 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 238, 1324, 1477, 1707, 2443, 2449, 2537, 2635 |
| Bse118I | RCCGGY | 3 cut(s) | 563, 834, 2104 |
| Bse1I | ACTGG | 5 cut(s) | 12, 1319, 1601, 1733, 2548 |
| Bse21I | CCTNAGG | 1 cut(s) | 948 |
| Bse3DI | GCAATG | 3 cut(s) | 558, 748, 1644 |
| Bse8I | GATNNNNATC | 2 cut(s) | 149, 2376 |
| BseAI | TCCGGA | 1 cut(s) | 1916 |
| BseBI | CCWGG | 2 cut(s) | 593, 2444 |
| BseDI | CCNNGG | 3 cut(s) | 802, 2437, 2516 |
| BseGI | GGATG | 8 cut(s) | 445, 625, 877, 1495, 1712, 1812, 1867, 1984 |
| BseJI | GATNNNNATC | 2 cut(s) | 149, 2376 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 238, 1324, 1477, 1707, 2443, 2449, 2537, 2635 |
| BseMI | GCAATG | 3 cut(s) | 558, 748, 1644 |
| BseMII | CTCAG | 2 cut(s) | 1464, 2168 |
| BseNI | ACTGG | 5 cut(s) | 12, 1319, 1601, 1733, 2548 |
| BseRI | GAGGAG | 4 cut(s) | 383, 794, 797, 1726 |
| BseSI | GKGCMC | 1 cut(s) | 591 |
| BseX3I | CGGCCG | 1 cut(s) | 2275 |
| BsgI | GTGCAG | 2 cut(s) | 2148, 2736 |
| Bsh1236I | CGCG | 1 cut(s) | 218 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2278 |
| BshFI | GGCC | 8 cut(s) | 224, 242, 728, 801, 2104, 2206, 2277, 2442 |
| BshNI | GGYRCC | 1 cut(s) | 746 |
| BsiEI | CGRYCG | 1 cut(s) | 2278 |
| BsiHKAI | GWGCWC | 3 cut(s) | 210, 591, 1191 |
| BsiHKCI | CYCGRG | 1 cut(s) | 231 |
| BsiWI | CGTACG | 1 cut(s) | 449 |
| BslFI | GGGAC | 1 cut(s) | 1729 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 238, 1324, 1477, 1707, 2443, 2449, 2537, 2635 |
| BsmAI | GTCTC | 2 cut(s) | 697, 1070 |
| BsmBI | CGTCTC | 1 cut(s) | 697 |
| BsmFI | GGGAC | 1 cut(s) | 1729 |
| BsmI | GAATGC | 2 cut(s) | 708, 1058 |
| BsnI | GGCC | 8 cut(s) | 224, 242, 728, 801, 2104, 2206, 2277, 2442 |
| BsoBI | CYCGRG | 1 cut(s) | 231 |
| Bsp1286I | GDGCHC | 3 cut(s) | 210, 591, 1191 |
| Bsp13I | TCCGGA | 1 cut(s) | 1916 |
| Bsp1407I | TGTACA | 3 cut(s) | 914, 1251, 1756 |
| Bsp143I | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2177 |
| Bsp19I | CCATGG | 1 cut(s) | 2516 |
| BspANI | GGCC | 8 cut(s) | 224, 242, 728, 801, 2104, 2206, 2277, 2442 |
| BspCNI | CTCAG | 2 cut(s) | 1465, 2169 |
| BspEI | TCCGGA | 1 cut(s) | 1916 |
| BspFNI | CGCG | 1 cut(s) | 218 |
| BspHI | TCATGA | 1 cut(s) | 1117 |
| BspLI | GGNNCC | 3 cut(s) | 163, 748, 2428 |
| BspMAI | CTGCAG | 1 cut(s) | 2098 |
| BspMI | ACCTGC | 2 cut(s) | 1303, 1402 |
| BspPI | GGATC | 2 cut(s) | 933, 1724 |
| BspT107I | GGYRCC | 1 cut(s) | 746 |
| BspTI | CTTAAG | 1 cut(s) | 1514 |
| BsrBI | CCGCTC | 2 cut(s) | 2181, 2211 |
| BsrDI | GCAATG | 3 cut(s) | 558, 748, 1644 |
| BsrFI | RCCGGY | 3 cut(s) | 563, 834, 2104 |
| BsrGI | TGTACA | 3 cut(s) | 914, 1251, 1756 |
| BsrI | ACTGG | 5 cut(s) | 12, 1319, 1601, 1733, 2548 |
| BssAI | RCCGGY | 3 cut(s) | 563, 834, 2104 |
| BssECI | CCNNGG | 3 cut(s) | 802, 2437, 2516 |
| BssMI | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| BssT1I | CCWWGG | 2 cut(s) | 2437, 2516 |
| Bst2UI | CCWGG | 2 cut(s) | 593, 2444 |
| Bst4CI | ACNGT | 8 cut(s) | 190, 419, 449, 1033, 1166, 1564, 2554, 2576 |
| Bst6I | CTCTTC | 3 cut(s) | 360, 1425, 2478 |
| BstAFI | CTTAAG | 1 cut(s) | 1514 |
| BstAUI | TGTACA | 3 cut(s) | 914, 1251, 1756 |
| BstDEI | CTNAG | 6 cut(s) | 948, 1182, 1473, 2177, 2417, 2735 |
| BstDSI | CCRYGG | 1 cut(s) | 2516 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2441 |
| BstF5I | GGATG | 8 cut(s) | 445, 625, 877, 1495, 1712, 1812, 1867, 1984 |
| BstFNI | CGCG | 1 cut(s) | 218 |
| BstH2I | RGCGCY | 1 cut(s) | 2264 |
| BstHHI | GCGC | 3 cut(s) | 470, 2134, 2263 |
| BstKTI | GATC | 7 cut(s) | 147, 403, 928, 1621, 1732, 2335, 2635 |
| BstMAI | GTCTC | 2 cut(s) | 697, 1070 |
| BstMBI | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| BstMCI | CGRYCG | 1 cut(s) | 2278 |
| BstNI | CCWGG | 2 cut(s) | 593, 2444 |
| BstSCI | CCNGG | 4 cut(s) | 138, 424, 591, 2442 |
| BstSFI | CTRYAG | 2 cut(s) | 1827, 2094 |
| BstSLI | GKGCMC | 1 cut(s) | 591 |
| BstUI | CGCG | 1 cut(s) | 218 |
| BstZI | CGGCCG | 1 cut(s) | 2275 |
| Bsu36I | CCTNAGG | 1 cut(s) | 948 |
| BsuI | GTATCC | 2 cut(s) | 978, 2636 |
| BsuRI | GGCC | 8 cut(s) | 224, 242, 728, 801, 2104, 2206, 2277, 2442 |
| BtgI | CCRYGG | 1 cut(s) | 2516 |
| BtsCI | GGATG | 8 cut(s) | 445, 625, 877, 1495, 1712, 1812, 1867, 1984 |
| BtsI | GCAGTG | 2 cut(s) | 1975, 2236 |
| BtsIMutI | CAGTG | 8 cut(s) | 5, 1326, 1740, 1975, 2051, 2236, 2550, 2572 |
| BveI | ACCTGC | 2 cut(s) | 1303, 1402 |
| CaiI | CAGNNNCTG | 1 cut(s) | 65 |
| CciI | TCATGA | 1 cut(s) | 1117 |
| CfoI | GCGC | 3 cut(s) | 470, 2134, 2263 |
| Cfr10I | RCCGGY | 3 cut(s) | 563, 834, 2104 |
| Cfr13I | GGNCC | 4 cut(s) | 726, 1153, 1998, 2102 |
| CseI | GACGC | 3 cut(s) | 65, 224, 714 |
| CsiI | ACCWGGT | 1 cut(s) | 591 |
| Csp6I | GTAC | 6 cut(s) | 450, 915, 1252, 1757, 2447, 2541 |
| CviAII | CATG | 7 cut(s) | 1006, 1118, 1274, 1466, 2497, 2517, 2756 |
| CviQI | GTAC | 6 cut(s) | 450, 915, 1252, 1757, 2447, 2541 |
| DdeI | CTNAG | 6 cut(s) | 948, 1182, 1473, 2177, 2417, 2735 |
| DpnI | GATC | 7 cut(s) | 146, 402, 927, 1620, 1731, 2334, 2634 |
| DpnII | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| DraI | TTTAAA | 1 cut(s) | 1104 |
| DriI | GACNNNNNGTC | 1 cut(s) | 61 |
| EaeI | YGGCCR | 4 cut(s) | 222, 240, 2275, 2440 |
| EagI | CGGCCG | 1 cut(s) | 2275 |
| Eam1104I | CTCTTC | 3 cut(s) | 360, 1425, 2478 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 61 |
| EarI | CTCTTC | 3 cut(s) | 360, 1425, 2478 |
| EciI | GGCGGA | 1 cut(s) | 518 |
| Ecl136II | GAGCTC | 2 cut(s) | 208, 1189 |
| EclXI | CGGCCG | 1 cut(s) | 2275 |
| Eco130I | CCWWGG | 2 cut(s) | 2437, 2516 |
| Eco24I | GRGCYC | 2 cut(s) | 210, 1191 |
| Eco47I | GGWCC | 2 cut(s) | 1153, 1998 |
| Eco52I | CGGCCG | 1 cut(s) | 2275 |
| Eco53kI | GAGCTC | 2 cut(s) | 208, 1189 |
| Eco57I | CTGAAG | 2 cut(s) | 1505, 1921 |
| Eco81I | CCTNAGG | 1 cut(s) | 948 |
| Eco88I | CYCGRG | 1 cut(s) | 231 |
| EcoICRI | GAGCTC | 2 cut(s) | 208, 1189 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2441 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1998 |
| EcoRI | GAATTC | 1 cut(s) | 2454 |
| EcoRII | CCWGG | 2 cut(s) | 591, 2442 |
| EcoT14I | CCWWGG | 2 cut(s) | 2437, 2516 |
| EcoT38I | GRGCYC | 2 cut(s) | 210, 1191 |
| ErhI | CCWWGG | 2 cut(s) | 2437, 2516 |
| Esp3I | CGTCTC | 1 cut(s) | 697 |
| FaeI | CATG | 7 cut(s) | 1009, 1121, 1277, 1469, 2500, 2520, 2759 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 257, 289 |
| FaqI | GGGAC | 1 cut(s) | 1729 |
| FatI | CATG | 7 cut(s) | 1005, 1117, 1273, 1465, 2496, 2516, 2755 |
| FauI | CCCGC | 3 cut(s) | 2093, 2179, 2204 |
| FbaI | TGATCA | 1 cut(s) | 400 |
| FblI | GTMKAC | 1 cut(s) | 1168 |
| FokI | GGATG | 8 cut(s) | 452, 632, 884, 1502, 1719, 1819, 1854, 1971 |
| FriOI | GRGCYC | 2 cut(s) | 210, 1191 |
| FspBI | CTAG | 7 cut(s) | 413, 584, 981, 1751, 2067, 2139, 2310 |
| GlaI | GCGC | 3 cut(s) | 469, 2133, 2262 |
| GsuI | CTGGAG | 3 cut(s) | 2138, 2212, 2285 |
| HaeII | RGCGCY | 1 cut(s) | 2264 |
| HaeIII | GGCC | 8 cut(s) | 224, 242, 728, 801, 2104, 2206, 2277, 2442 |
| HgaI | GACGC | 3 cut(s) | 65, 224, 714 |
| HhaI | GCGC | 3 cut(s) | 470, 2134, 2263 |
| Hin1II | CATG | 7 cut(s) | 1009, 1121, 1277, 1469, 2500, 2520, 2759 |
| Hin6I | GCGC | 3 cut(s) | 468, 2132, 2261 |
| HinP1I | GCGC | 3 cut(s) | 468, 2132, 2261 |
| HincII | GTYRAC | 2 cut(s) | 2494, 2584 |
| HindII | GTYRAC | 2 cut(s) | 2494, 2584 |
| HindIII | AAGCTT | 1 cut(s) | 1511 |
| HphI | GGTGA | 7 cut(s) | 431, 967, 1592, 2426, 2489, 2653, 2780 |
| Hpy166II | GTNNAC | 5 cut(s) | 589, 1169, 1876, 2494, 2584 |
| Hpy8I | GTNNAC | 5 cut(s) | 589, 1169, 1876, 2494, 2584 |
| Hpy99I | CGWCG | 2 cut(s) | 173, 218 |
| HpyAV | CCTTC | 4 cut(s) | 1237, 1480, 2417, 2744 |
| HpyCH4III | ACNGT | 8 cut(s) | 190, 419, 449, 1033, 1166, 1564, 2554, 2576 |
| HpyCH4IV | ACGT | 2 cut(s) | 246, 2787 |
| HpyF3I | CTNAG | 6 cut(s) | 948, 1182, 1473, 2177, 2417, 2735 |
| HpySE526I | ACGT | 2 cut(s) | 246, 2787 |
| Hsp92II | CATG | 7 cut(s) | 1009, 1121, 1277, 1469, 2500, 2520, 2759 |
| HspAI | GCGC | 3 cut(s) | 468, 2132, 2261 |
| Kpn2I | TCCGGA | 1 cut(s) | 1916 |
| KroI | GCCGGC | 1 cut(s) | 563 |
| KroNI | GCCGGC | 1 cut(s) | 565 |
| Ksp22I | TGATCA | 1 cut(s) | 400 |
| Kzo9I | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| LmnI | GCTCC | 4 cut(s) | 167, 1186, 1654, 1713 |
| LweI | GCATC | 7 cut(s) | 693, 757, 1698, 1867, 1942, 2289, 2641 |
| MabI | ACCWGGT | 1 cut(s) | 591 |
| MaeI | CTAG | 7 cut(s) | 413, 584, 981, 1751, 2067, 2139, 2310 |
| MaeII | ACGT | 2 cut(s) | 246, 2787 |
| MaeIII | GTNAC | 4 cut(s) | 973, 1160, 1635, 2393 |
| MalI | GATC | 7 cut(s) | 146, 402, 927, 1620, 1731, 2334, 2634 |
| MbiI | CCGCTC | 2 cut(s) | 2181, 2211 |
| MboI | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| MboII | GAAGA | 9 cut(s) | 331, 377, 520, 1442, 1459, 2338, 2372, 2492, 2495 |
| MfeI | CAATTG | 2 cut(s) | 543, 2502 |
| MhlI | GDGCHC | 3 cut(s) | 210, 591, 1191 |
| MlsI | TGGCCA | 2 cut(s) | 242, 2442 |
| MluI | ACGCGT | 1 cut(s) | 216 |
| MluNI | TGGCCA | 2 cut(s) | 242, 2442 |
| MlyI | GAGTC | 5 cut(s) | 71, 321, 418, 463, 1235 |
| MmeI | TCCRAC | 3 cut(s) | 849, 1254, 1984 |
| Mox20I | TGGCCA | 2 cut(s) | 242, 2442 |
| MroI | TCCGGA | 1 cut(s) | 1916 |
| MroNI | GCCGGC | 1 cut(s) | 563 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1269, 1934 |
| MscI | TGGCCA | 2 cut(s) | 242, 2442 |
| MseI | TTAA | 9 cut(s) | 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2301 |
| MslI | CAYNNNNRTG | 2 cut(s) | 584, 1805 |
| Msp20I | TGGCCA | 2 cut(s) | 242, 2442 |
| MspA1I | CMGCKG | 1 cut(s) | 1788 |
| MspCI | CTTAAG | 1 cut(s) | 1514 |
| MspR9I | CCNGG | 4 cut(s) | 140, 426, 593, 2444 |
| MunI | CAATTG | 2 cut(s) | 543, 2502 |
| Mva1269I | GAATGC | 2 cut(s) | 708, 1058 |
| MvaI | CCWGG | 2 cut(s) | 593, 2444 |
| MvnI | CGCG | 1 cut(s) | 218 |
| NaeI | GCCGGC | 1 cut(s) | 565 |
| NciI | CCSGG | 2 cut(s) | 140, 426 |
| NcoI | CCATGG | 1 cut(s) | 2516 |
| NdeII | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| NgoMIV | GCCGGC | 1 cut(s) | 563 |
| NlaIII | CATG | 7 cut(s) | 1009, 1121, 1277, 1469, 2500, 2520, 2759 |
| NlaIV | GGNNCC | 3 cut(s) | 163, 748, 2428 |
| NmeAIII | GCCGAG | 2 cut(s) | 138, 2232 |
| NmuCI | GTSAC | 1 cut(s) | 973 |
| OliI | CACNNNNGTG | 1 cut(s) | 584 |
| PagI | TCATGA | 1 cut(s) | 1117 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 165, 210, 222, 2455 |
| PctI | GAATGC | 2 cut(s) | 708, 1058 |
| PdiI | GCCGGC | 1 cut(s) | 565 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1269, 1934 |
| PfeI | GAWTC | 5 cut(s) | 227, 340, 1197, 1213, 1583 |
| Pfl23II | CGTACG | 1 cut(s) | 449 |
| PflFI | GACNNNGTC | 1 cut(s) | 190 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2537 |
| PfoI | TCCNGGA | 1 cut(s) | 138 |
| PleI | GAGTC | 5 cut(s) | 70, 321, 417, 462, 1234 |
| PpsI | GAGTC | 5 cut(s) | 70, 321, 417, 462, 1234 |
| PpuMI | RGGWCCY | 1 cut(s) | 1998 |
| PsiI | TTATAA | 1 cut(s) | 1568 |
| Psp124BI | GAGCTC | 2 cut(s) | 210, 1191 |
| Psp5II | RGGWCCY | 1 cut(s) | 1998 |
| Psp6I | CCWGG | 2 cut(s) | 591, 2442 |
| PspGI | CCWGG | 2 cut(s) | 591, 2442 |
| PspLI | CGTACG | 1 cut(s) | 449 |
| PspN4I | GGNNCC | 3 cut(s) | 163, 748, 2428 |
| PspPI | GGNCC | 4 cut(s) | 726, 1153, 1998, 2102 |
| PspPPI | RGGWCCY | 1 cut(s) | 1998 |
| PstI | CTGCAG | 1 cut(s) | 2098 |
| PstNI | CAGNNNCTG | 1 cut(s) | 65 |
| PsyI | GACNNNGTC | 1 cut(s) | 190 |
| PvuII | CAGCTG | 1 cut(s) | 1788 |
| RsaI | GTAC | 6 cut(s) | 451, 916, 1253, 1758, 2448, 2542 |
| RsaNI | GTAC | 6 cut(s) | 450, 915, 1252, 1757, 2447, 2541 |
| RseI | CAYNNNNRTG | 2 cut(s) | 584, 1805 |
| SacI | GAGCTC | 2 cut(s) | 210, 1191 |
| SaqAI | TTAA | 9 cut(s) | 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2301 |
| Sau3AI | GATC | 7 cut(s) | 144, 400, 925, 1618, 1729, 2332, 2632 |
| Sau96I | GGNCC | 4 cut(s) | 726, 1153, 1998, 2102 |
| SchI | GAGTC | 5 cut(s) | 71, 321, 418, 463, 1235 |
| ScrFI | CCNGG | 4 cut(s) | 140, 426, 593, 2444 |
| SduI | GDGCHC | 3 cut(s) | 210, 591, 1191 |
| SexAI | ACCWGGT | 1 cut(s) | 591 |
| SfaNI | GCATC | 7 cut(s) | 693, 757, 1698, 1867, 1942, 2289, 2641 |
| SfcI | CTRYAG | 2 cut(s) | 1827, 2094 |
| SinI | GGWCC | 2 cut(s) | 1153, 1998 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 584, 1805 |
| SmlI | CTYRAG | 4 cut(s) | 25, 809, 1514, 1693 |
| SmoI | CTYRAG | 4 cut(s) | 25, 809, 1514, 1693 |
| SspI | AATATT | 1 cut(s) | 1068 |
| SspMI | CTAG | 7 cut(s) | 413, 584, 981, 1751, 2067, 2139, 2310 |
| SstI | GAGCTC | 2 cut(s) | 210, 1191 |
| StyD4I | CCNGG | 4 cut(s) | 138, 424, 591, 2442 |
| StyI | CCWWGG | 2 cut(s) | 2437, 2516 |
| TaaI | ACNGT | 8 cut(s) | 190, 419, 449, 1033, 1166, 1564, 2554, 2576 |
| TaiI | ACGT | 2 cut(s) | 249, 2790 |
| TaqI | TCGA | 3 cut(s) | 363, 1290, 2463 |
| TatI | WGTACW | 3 cut(s) | 914, 1251, 1756 |
| TauI | GCSGC | 4 cut(s) | 356, 468, 2241, 2280 |
| TfiI | GAWTC | 5 cut(s) | 227, 340, 1197, 1213, 1583 |
| Tru1I | TTAA | 9 cut(s) | 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2301 |
| Tru9I | TTAA | 9 cut(s) | 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2301 |
| TscAI | CASTG | 8 cut(s) | 12, 1326, 1740, 1982, 2051, 2236, 2557, 2579 |
| TseFI | GTSAC | 1 cut(s) | 973 |
| Tsp45I | GTSAC | 1 cut(s) | 973 |
| TspGWI | ACGGA | 2 cut(s) | 1225, 2346 |
| TspRI | CASTG | 8 cut(s) | 12, 1326, 1740, 1982, 2051, 2236, 2557, 2579 |
| Tth111I | GACNNNGTC | 1 cut(s) | 190 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2537 |
| Vha464I | CTTAAG | 1 cut(s) | 1514 |
| VneI | GTGCAC | 1 cut(s) | 587 |
| VpaK11BI | GGWCC | 2 cut(s) | 1153, 1998 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2441 |
| XapI | RAATTY | 6 cut(s) | 671, 893, 1313, 1385, 1545, 2454 |
| XbaI | TCTAGA | 2 cut(s) | 412, 2309 |
| XmiI | GTMKAC | 1 cut(s) | 1168 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1269, 1934 |
| XspI | CTAG | 7 cut(s) | 413, 584, 981, 1751, 2067, 2139, 2310 |