ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 661 |
| AciI | CCGC | 1 cut(s) | 350 |
| AclI | AACGTT | 1 cut(s) | 629 |
| AcsI | RAATTY | 2 cut(s) | 266, 702 |
| AcuI | CTGAAG | 2 cut(s) | 276, 413 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 185, 313 |
| AgsI | TTSAA | 3 cut(s) | 131, 266, 311 |
| AjnI | CCWGG | 4 cut(s) | 69, 184, 384, 540 |
| AleI | CACNNNNGTG | 1 cut(s) | 386 |
| AluBI | AGCT | 5 cut(s) | 36, 105, 149, 482, 523 |
| AluI | AGCT | 5 cut(s) | 36, 105, 149, 482, 523 |
| Alw21I | GWGCWC | 1 cut(s) | 337 |
| Alw26I | GTCTC | 1 cut(s) | 71 |
| AoxI | GGCC | 1 cut(s) | 421 |
| ApeKI | GCWGC | 1 cut(s) | 79 |
| ApoI | RAATTY | 2 cut(s) | 266, 702 |
| AsuHPI | GGTGA | 2 cut(s) | 243, 484 |
| BauI | CACGAG | 1 cut(s) | 199 |
| Bbv12I | GWGCWC | 1 cut(s) | 337 |
| BbvI | GCAGC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 4 cut(s) | 71, 186, 386, 542 |
| BcoDI | GTCTC | 1 cut(s) | 71 |
| BfaI | CTAG | 3 cut(s) | 321, 327, 471 |
| BisI | GCNGC | 1 cut(s) | 80 |
| BlsI | GCNGC | 1 cut(s) | 81 |
| Bme1390I | CCNGG | 4 cut(s) | 71, 186, 386, 542 |
| BmrFI | CCNGG | 4 cut(s) | 71, 186, 386, 542 |
| BmsI | GCATC | 2 cut(s) | 255, 612 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 557, 589 |
| BsaJI | CCNNGG | 2 cut(s) | 384, 465 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 185, 313 |
| Bse1I | ACTGG | 1 cut(s) | 495 |
| BseBI | CCWGG | 4 cut(s) | 71, 186, 386, 542 |
| BseDI | CCNNGG | 2 cut(s) | 384, 465 |
| BseGI | GGATG | 2 cut(s) | 103, 127 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 185, 313 |
| BseNI | ACTGG | 1 cut(s) | 495 |
| BseXI | GCAGC | 1 cut(s) | 66 |
| BseYI | CCCAGC | 1 cut(s) | 365 |
| BsgI | GTGCAG | 1 cut(s) | 65 |
| BshFI | GGCC | 1 cut(s) | 423 |
| BsiHKAI | GWGCWC | 1 cut(s) | 337 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 185, 313 |
| BsmAI | GTCTC | 1 cut(s) | 71 |
| BsnI | GGCC | 1 cut(s) | 423 |
| Bsp1286I | GDGCHC | 1 cut(s) | 337 |
| Bsp143I | GATC | 1 cut(s) | 216 |
| BspACI | CCGC | 1 cut(s) | 350 |
| BspANI | GGCC | 1 cut(s) | 423 |
| BspHI | TCATGA | 1 cut(s) | 697 |
| BsrI | ACTGG | 1 cut(s) | 495 |
| BssECI | CCNNGG | 2 cut(s) | 384, 465 |
| BssMI | GATC | 1 cut(s) | 216 |
| BssSI | CACGAG | 1 cut(s) | 199 |
| BssT1I | CCWWGG | 1 cut(s) | 465 |
| Bst2BI | CACGAG | 1 cut(s) | 199 |
| Bst2UI | CCWGG | 4 cut(s) | 71, 186, 386, 542 |
| Bst4CI | ACNGT | 2 cut(s) | 90, 666 |
| Bst6I | CTCTTC | 2 cut(s) | 306, 558 |
| BstC8I | GCNNGC | 1 cut(s) | 575 |
| BstDEI | CTNAG | 1 cut(s) | 37 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 183, 311 |
| BstF5I | GGATG | 2 cut(s) | 103, 127 |
| BstKTI | GATC | 1 cut(s) | 219 |
| BstMAI | GTCTC | 1 cut(s) | 71 |
| BstMBI | GATC | 1 cut(s) | 216 |
| BstNI | CCWGG | 4 cut(s) | 71, 186, 386, 542 |
| BstSCI | CCNGG | 4 cut(s) | 69, 184, 384, 540 |
| BstV1I | GCAGC | 1 cut(s) | 66 |
| BsuRI | GGCC | 1 cut(s) | 423 |
| BtsCI | GGATG | 2 cut(s) | 103, 127 |
| BtsI | GCAGTG | 2 cut(s) | 337, 360 |
| BtsIMutI | CAGTG | 4 cut(s) | 337, 360, 460, 502 |
| Cac8I | GCNNGC | 1 cut(s) | 575 |
| CciI | TCATGA | 1 cut(s) | 697 |
| CsiI | ACCWGGT | 1 cut(s) | 540 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 454, 489 |
| CviAII | CATG | 3 cut(s) | 548, 656, 698 |
| DdeI | CTNAG | 1 cut(s) | 37 |
| DpnI | GATC | 1 cut(s) | 218 |
| DpnII | GATC | 1 cut(s) | 216 |
| Eam1104I | CTCTTC | 2 cut(s) | 306, 558 |
| EarI | CTCTTC | 2 cut(s) | 306, 558 |
| Eco130I | CCWWGG | 1 cut(s) | 465 |
| Eco57I | CTGAAG | 2 cut(s) | 276, 413 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 183, 311 |
| EcoRII | CCWGG | 4 cut(s) | 69, 184, 384, 540 |
| EcoT14I | CCWWGG | 1 cut(s) | 465 |
| ErhI | CCWWGG | 1 cut(s) | 465 |
| FaeI | CATG | 3 cut(s) | 551, 659, 701 |
| FaiI | YATR | 7 cut(s) | 11, 206, 440, 549, 657, 699, 709 |
| FatI | CATG | 3 cut(s) | 547, 655, 697 |
| FblI | GTMKAC | 1 cut(s) | 661 |
| Fnu4HI | GCNGC | 1 cut(s) | 80 |
| FokI | GGATG | 2 cut(s) | 110, 134 |
| Fsp4HI | GCNGC | 1 cut(s) | 80 |
| FspBI | CTAG | 3 cut(s) | 321, 327, 471 |
| GluI | GCNGC | 1 cut(s) | 80 |
| GsaI | CCCAGC | 1 cut(s) | 369 |
| HaeIII | GGCC | 1 cut(s) | 423 |
| Hin1II | CATG | 3 cut(s) | 551, 659, 701 |
| HindIII | AAGCTT | 3 cut(s) | 34, 147, 521 |
| HinfI | GANTC | 1 cut(s) | 278 |
| HphI | GGTGA | 2 cut(s) | 243, 484 |
| Hpy166II | GTNNAC | 1 cut(s) | 662 |
| Hpy188III | TCNNGA | 4 cut(s) | 59, 173, 272, 698 |
| Hpy8I | GTNNAC | 1 cut(s) | 662 |
| HpyAV | CCTTC | 3 cut(s) | 125, 388, 413 |
| HpyCH4III | ACNGT | 2 cut(s) | 90, 666 |
| HpyCH4IV | ACGT | 1 cut(s) | 629 |
| HpyCH4V | TGCA | 3 cut(s) | 82, 674, 686 |
| HpyF3I | CTNAG | 1 cut(s) | 37 |
| HpySE526I | ACGT | 1 cut(s) | 629 |
| Hsp92II | CATG | 3 cut(s) | 551, 659, 701 |
| Kzo9I | GATC | 1 cut(s) | 216 |
| Lsp1109I | GCAGC | 1 cut(s) | 66 |
| LweI | GCATC | 2 cut(s) | 255, 612 |
| MabI | ACCWGGT | 1 cut(s) | 540 |
| MaeI | CTAG | 3 cut(s) | 321, 327, 471 |
| MaeII | ACGT | 1 cut(s) | 629 |
| MaeIII | GTNAC | 3 cut(s) | 272, 490, 640 |
| MalI | GATC | 1 cut(s) | 218 |
| MboI | GATC | 1 cut(s) | 216 |
| MboII | GAAGA | 4 cut(s) | 226, 244, 323, 575 |
| MfeI | CAATTG | 1 cut(s) | 681 |
| MhlI | GDGCHC | 1 cut(s) | 337 |
| MluCI | AATT | 5 cut(s) | 15, 266, 593, 681, 702 |
| MmeI | TCCRAC | 3 cut(s) | 75, 99, 612 |
| MnlI | CCTC | 7 cut(s) | 39, 189, 286, 307, 339, 498, 559 |
| MseI | TTAA | 1 cut(s) | 432 |
| MslI | CAYNNNNRTG | 1 cut(s) | 386 |
| MspR9I | CCNGG | 4 cut(s) | 71, 186, 386, 542 |
| MunI | CAATTG | 1 cut(s) | 681 |
| MvaI | CCWGG | 4 cut(s) | 71, 186, 386, 542 |
| NdeII | GATC | 1 cut(s) | 216 |
| NlaIII | CATG | 3 cut(s) | 551, 659, 701 |
| NmuCI | GTSAC | 2 cut(s) | 272, 490 |
| OliI | CACNNNNGTG | 1 cut(s) | 386 |
| PagI | TCATGA | 1 cut(s) | 697 |
| PfeI | GAWTC | 1 cut(s) | 278 |
| PfoI | TCCNGGA | 1 cut(s) | 69 |
| PkrI | GCNGC | 1 cut(s) | 81 |
| Psp1406I | AACGTT | 1 cut(s) | 629 |
| Psp6I | CCWGG | 4 cut(s) | 69, 184, 384, 540 |
| PspFI | CCCAGC | 1 cut(s) | 365 |
| PspGI | CCWGG | 4 cut(s) | 69, 184, 384, 540 |
| RseI | CAYNNNNRTG | 1 cut(s) | 386 |
| SaqAI | TTAA | 1 cut(s) | 432 |
| SatI | GCNGC | 1 cut(s) | 80 |
| Sau3AI | GATC | 1 cut(s) | 216 |
| ScrFI | CCNGG | 4 cut(s) | 71, 186, 386, 542 |
| SduI | GDGCHC | 1 cut(s) | 337 |
| SexAI | ACCWGGT | 1 cut(s) | 540 |
| SfaNI | GCATC | 2 cut(s) | 255, 612 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 386 |
| Sse9I | AATT | 5 cut(s) | 15, 266, 593, 681, 702 |
| SsiI | CCGC | 1 cut(s) | 350 |
| SspMI | CTAG | 3 cut(s) | 321, 327, 471 |
| StyD4I | CCNGG | 4 cut(s) | 69, 184, 384, 540 |
| StyI | CCWWGG | 1 cut(s) | 465 |
| TaaI | ACNGT | 2 cut(s) | 90, 666 |
| TaiI | ACGT | 1 cut(s) | 632 |
| TaqII | GACCGA | 1 cut(s) | 44 |
| TasI | AATT | 5 cut(s) | 15, 266, 593, 681, 702 |
| TfiI | GAWTC | 1 cut(s) | 278 |
| Tru1I | TTAA | 1 cut(s) | 432 |
| Tru9I | TTAA | 1 cut(s) | 432 |
| TscAI | CASTG | 4 cut(s) | 337, 367, 460, 502 |
| TseFI | GTSAC | 2 cut(s) | 272, 490 |
| TseI | GCWGC | 1 cut(s) | 79 |
| Tsp45I | GTSAC | 2 cut(s) | 272, 490 |
| TspDTI | ATGAA | 4 cut(s) | 114, 536, 663, 686 |
| TspRI | CASTG | 4 cut(s) | 337, 367, 460, 502 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 183, 311 |
| XapI | RAATTY | 2 cut(s) | 266, 702 |
| XmiI | GTMKAC | 1 cut(s) | 661 |
| XspI | CTAG | 3 cut(s) | 321, 327, 471 |