Rmu_sc0023925.1_g000004

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023925.1
OG0000202
Physical Location & Seq
Reverse (-)
5438 .. 14025
8588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023925.1_g000004.1.cds

Sequence Viewer

Length: 3270 bp
atggctgccaaggattttgctgaattccagtggaagaagttcttgaggtctctgttagaggcagagtctgaattatcaggttccataaatctcatttcttacttcaaaacaatcaaacagaagttaatagaaacagagacgattcctttttccgagatagctgcagctgcagggatggcattgaatatggtgtcatgggaaaggaatacttacattctggtttaccgttatctcttggagctcaactacgcattggcagagtgccgaatctttgcaaaggagttcaaggagcttaacaagaagatcttgcccttgaatccttccacagtttatttcatcctgaagatgaagaagcggctgacggcactgagcagtaagtttgacattttggtgcgtagcagcccatcaacgtctgtttctgtcctgagggagaaaaaagagaggaacgcagtcgaatgtgtgggatttacagagaaagatatagtggtgataaagaagaactcagaagaaactgtggtgttttctgatgtacgatttgggagacagtatagtggttcactgacgcttagggacttccacttgctcagtgacggtgcaactggaaatggattcacgtccattgggatagtcggcatggctggtgtgggtaagtccactttcttattaaaagaaattttggagtggaatgacatacgctctgagttttctcctatcattcagttatgtttgtctgatataatgcaagaaaacaatgtagtagacggtactagtgccaatggggtcagcatgagcattgtgaaactcattcttcaaaagttgggggaggaggaaactggtatttctggcctaggactcacttcgctcttggaaaggctctacagtcttttatcaggtaaaaagtatttgattgcgttggatgatgtgtttaatgctatggaattctattcggatttgggtaatgaccgtgggaaattcgggaatcggttatcgcatggattgcctaaggatagtggtggtgtagtcattgtcattagtagtaaaccagaagtagctctcgatatggtggggcttcgccaaaattctgtgcataagcggctgaccacactcaaaaggaagtctggagtacggtcgacaacttcagtggaacggcaagaccatcaaacaccaatagataacagccccacatcgatttctacgccgaaagagaagacgaatgttgtagaagaatgtttgccattaacagagaaggcaaagaaaatttcaccagaaaatgtgctactaattcccaatgtagggaggtccaggaaccaaagggattttagggactccagcttgcttgttggtgatccagccggaattggatttacggccattgggatagttggcatggcaggtgttggtaagtccactttcgtgcacaaggtcttgaaaactgtgtggttcgaatttgatcctatagtttggttatgcttgtcggatataatcaaagctaaaaaccaagtgccggatgctactgctgccactgatcaggagatcagcattagcattgtgacatgtatccttgaaatgttgggggtctccatagacaagaaccttacccttgccgagctcttggaaaggctctaccatgttttattaggtaaaaggtatttgattgtgcttgatgatgtttgggacattatggagttctactcggatttatgttttaaactcccagaaggtgaaaaaattcaggatcacttatcacacggattgcctaaagactctggtggtacggtcattgtcactactaggatgacggaagtagcgcgagatatggtgggctttggccaaaataagttgttccttgttctgccattggagagagaagagtgcttttccaactttaacagctttttacaccatgaaaaatacaggtgcggtgaaggaacacgctttggtaagtccaacatggaaactgtgaaaaaggttcatgatgagattctgcatcaagtttgtggcctaccatcaattgccaagactctggcagacttgatcattgcaaatcagattggtcaaaatgattataataggtctgccctgaagaagatgttgatacctcatgaaattcgtattcaatgttcatgggtgactgttgcgacaaaagacaagaaagcgaaatggattcaggataaaaccgaagtaccaaaatgtccaatatttgtgtttattgatttaagaagtgaccaagtaggagatcttgctgaagaccttcgctacttgcttaatcgtgagcaggtcattgctgttctgcacgagagtttctataacaagtataaattatcgacagtgaacccagatagggcgctgactgaggtttatggtgctctagcaacgcttaagggtgtaggacataactttgctgacgaaattcaaaagaagatgacaattataattgttggtgaggatgtttttataaactggtttctgggagttttctgtgatctaaatttgccagactcaccctccattgttcccatagcactcggaatcaagagtaacatcccattctcctttggatgggagtttgatgctatgaaaccagtgaacaagatcttggagacggttctaaatcatacaaaacagatcaaagctgacaggaccttgaacaggttacttgcaaaagtagaggttttaaaacatcatggtgatcagtgggaaatgcttagcattcctagtagcattaggtcaattgtttgtctcaacttgcctagctttgttggtggactaactccttggacaacagcaaatttgaagaaagatcacagaaaaggcttgacctcatctttcacagatgatggacgtcttgaaattattggttcccgagatgatttactttctccgaagaaaaggtggattcgccttgatcaggtcatttaccctctagcctcttgctatcgcttcctcctctccatggcctccatagatgccgtcacagcaagctttgctgcttctcttactctcacagcgggtggtagcgtctcggatctgggacagatttgcggtggtcctgtacgcagatcctcccaatattttctacttggcaaacctttgatcaatgatgacaatgatgtgcctcttgtcactctgaagaagaagacgagaagactactggaaggaagaacaagagccaacgtcccagtaactgcaattggaggttcgatttctagccctagagataagggcaaaggaaagcctttaggctttcctggtctcttgcctaccacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

1089

Amino Acids

121.23

Weight (kDa)

8.73

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2067, 2432, 2456
AarI CACCTGC 1 cut(s) 1382
AatII GACGTC 1 cut(s) 2845
Acc36I ACCTGC 2 cut(s) 1382, 2266
AccI GTMKAC 2 cut(s) 759, 1130
AccII CGCG 1 cut(s) 1810
AciI CCGC 5 cut(s) 355, 1093, 1920, 3008, 3042
AclWI GGATC 5 cut(s) 1340, 1445, 1743, 3033, 3054
AcoI YGGCCR 2 cut(s) 1368, 1828
AcuI CTGAAG 5 cut(s) 362, 1122, 2102, 2265, 3149
AcyI GRCGYC 1 cut(s) 2842
AfaI GTAC 6 cut(s) 531, 766, 1125, 1774, 2184, 3054
AfiI CCNNNNNNNGG 8 cut(s) 1292, 1293, 1504, 2341, 2559, 2649, 2908, 2953
AflII CTTAAG 1 cut(s) 2378
AflIII ACRYGT 1 cut(s) 1553
AhlI ACTAGT 1 cut(s) 767
AjiI CACGTC 1 cut(s) 615
AjnI CCWGG 2 cut(s) 1301, 3247
AjuI GAANNNNNNNTTGG 2 cut(s) 2579, 2611
AleI CACNNNNGTG 1 cut(s) 1412
Alw21I GWGCWC 4 cut(s) 243, 1419, 1611, 2368
Alw26I GTCTC 8 cut(s) 54, 131, 535, 1582, 2594, 2744, 3025, 3257
Alw44I GTGCAC 1 cut(s) 1415
AlwI GGATC 5 cut(s) 1340, 1445, 1743, 3033, 3054
AlwNI CAGNNNCTG 2 cut(s) 68, 3185
Ama87I CYCGRG 1 cut(s) 2862
AoxI GGCC 5 cut(s) 844, 1368, 1828, 1999, 2955
ApaLI GTGCAC 1 cut(s) 1415
ApeKI GCWGC 7 cut(s) 5, 161, 164, 167, 399, 1517, 2987
Asp700I GAANNNNTTC 3 cut(s) 38, 1728, 2250
AspA2I CCTAGG 1 cut(s) 847
AspLEI GCGC 2 cut(s) 1810, 2347
AspS9I GGNCC 3 cut(s) 1299, 2640, 3047
AsuHPI GGTGA 9 cut(s) 499, 1254, 1355, 1733, 1934, 2140, 2453, 2493, 2699
AsuII TTCGAA 1 cut(s) 1443
AvaI CYCGRG 1 cut(s) 2862
AvaII GGWCC 3 cut(s) 1299, 2640, 3047
AvrII CCTAGG 1 cut(s) 847
AxyI CCTNAGG 2 cut(s) 425, 1002
BaeGI GKGCMC 1 cut(s) 1419
BaeI ACNNNNGTAYC 2 cut(s) 2166, 2199
BalI TGGCCA 1 cut(s) 1830
BanII GRGCYC 2 cut(s) 243, 1611
BauI CACGAG 1 cut(s) 2294
BbsI GAAGAC 4 cut(s) 1214, 2253, 3143, 3151
Bbv12I GWGCWC 4 cut(s) 243, 1419, 1611, 2368
BbvI GCAGC 6 cut(s) 148, 154, 176, 411, 1504, 2974
BccI CCATC 6 cut(s) 169, 412, 1164, 2014, 2553, 2831
BceAI ACGGC 4 cut(s) 378, 1163, 1383, 2954
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 1303, 3249
BciVI GTATCC 1 cut(s) 1568
BclI TGATCA 5 cut(s) 1525, 2034, 2689, 2905, 3093
BcoDI GTCTC 8 cut(s) 54, 131, 535, 1582, 2594, 2744, 3025, 3257
BcuI ACTAGT 1 cut(s) 767
BfaI CTAG 9 cut(s) 768, 848, 1791, 2369, 2715, 2751, 2924, 3207, 3213
BfmI CTRYAG 4 cut(s) 162, 168, 877, 1455
BfoI RGCGCY 1 cut(s) 2348
BfrI CTTAAG 1 cut(s) 2378
BfuAI ACCTGC 2 cut(s) 1382, 2266
BfuI GTATCC 1 cut(s) 1568
BglII AGATCT 3 cut(s) 303, 2236, 2592
BisI GCNGC 9 cut(s) 6, 162, 165, 168, 356, 400, 1094, 1518, 2988
BlnI CCTAGG 1 cut(s) 847
BlpI GCTNAGC 1 cut(s) 2705
BlsI GCNGC 9 cut(s) 7, 163, 166, 169, 357, 401, 1095, 1519, 2989
Bme1390I CCNGG 2 cut(s) 1303, 3249
Bme18I GGWCC 3 cut(s) 1299, 2640, 3047
BmeT110I CYCGRG 1 cut(s) 2862
BmgBI CACGTC 1 cut(s) 615
BmgT120I GGNCC 3 cut(s) 1299, 2640, 3047
BmiI GGNNCC 3 cut(s) 82, 1307, 2860
BmrFI CCNGG 2 cut(s) 1303, 3249
BmrI ACTGGG 1 cut(s) 3173
BmsI GCATC 4 cut(s) 1498, 1996, 2560, 2956
BmuI ACTGGG 1 cut(s) 3173
BpiI GAAGAC 4 cut(s) 1214, 2253, 3143, 3151
BplI GAGNNNNNCTC 2 cut(s) 1676, 1708
BpmI CTGGAG 2 cut(s) 1140, 1312
Bpu10I CCTNAGC 1 cut(s) 566
Bpu1102I GCTNAGC 1 cut(s) 2705
Bpu14I TTCGAA 1 cut(s) 1443
BpuEI CTTGAG 1 cut(s) 64
Bsa29I ATCGAT 1 cut(s) 1187
BsaHI GRCGYC 1 cut(s) 2842
BsaI GGTCTC 3 cut(s) 54, 1582, 3257
BsaJI CCNNGG 5 cut(s) 9, 847, 964, 2774, 2952
BsaXI ACNNNNNCTCC 2 cut(s) 2489, 2519
Bsc4I CCNNNNNNNGG 8 cut(s) 1292, 1293, 1504, 2341, 2559, 2649, 2908, 2953
Bse1I ACTGG 7 cut(s) 28, 604, 838, 2465, 2582, 3156, 3179
Bse21I CCTNAGG 2 cut(s) 425, 1002
Bse3DI GCAATG 2 cut(s) 2037, 2280
BseBI CCWGG 2 cut(s) 1303, 3249
BseCI ATCGAT 1 cut(s) 1187
BseDI CCNNGG 5 cut(s) 9, 847, 964, 2774, 2952
BseGI GGATG 8 cut(s) 180, 336, 922, 1513, 1800, 2452, 2541, 2564
BseLI CCNNNNNNNGG 8 cut(s) 1292, 1293, 1504, 2341, 2559, 2649, 2908, 2953
BseMI GCAATG 2 cut(s) 2037, 2280
BseMII CTCAG 6 cut(s) 359, 416, 516, 598, 690, 2343
BseNI ACTGG 7 cut(s) 28, 604, 838, 2465, 2582, 3156, 3179
BseRI GAGGAG 2 cut(s) 839, 2936
BseSI GKGCMC 1 cut(s) 1419
BseXI GCAGC 6 cut(s) 148, 154, 176, 411, 1504, 2974
BsgI GTGCAG 1 cut(s) 2276
Bsh1236I CGCG 1 cut(s) 1810
Bsh1285I CGRYCG 1 cut(s) 1130
BshFI GGCC 5 cut(s) 846, 1370, 1830, 2001, 2957
BshVI ATCGAT 1 cut(s) 1187
BsiEI CGRYCG 1 cut(s) 1130
BsiHKAI GWGCWC 4 cut(s) 243, 1419, 1611, 2368
BsiHKCI CYCGRG 1 cut(s) 2862
BsiSI CCGG 2 cut(s) 1353, 1505
BslFI GGGAC 5 cut(s) 584, 1337, 1688, 3045, 3161
BslI CCNNNNNNNGG 8 cut(s) 1292, 1293, 1504, 2341, 2559, 2649, 2908, 2953
BsmAI GTCTC 8 cut(s) 54, 131, 535, 1582, 2594, 2744, 3025, 3257
BsmBI CGTCTC 3 cut(s) 131, 2594, 3025
BsmFI GGGAC 5 cut(s) 584, 1337, 1688, 3045, 3161
BsmI GAATGC 1 cut(s) 2709
BsnI GGCC 5 cut(s) 846, 1370, 1830, 2001, 2957
Bso31I GGTCTC 3 cut(s) 54, 1582, 3257
BsoBI CYCGRG 1 cut(s) 2862
Bsp119I TTCGAA 1 cut(s) 1443
Bsp1286I GDGCHC 4 cut(s) 243, 1419, 1611, 2368
Bsp1720I GCTNAGC 1 cut(s) 2705
Bsp19I CCATGG 1 cut(s) 2952
BspACI CCGC 5 cut(s) 355, 1093, 1920, 3008, 3042
BspANI GGCC 5 cut(s) 846, 1370, 1830, 2001, 2957
BspCNI CTCAG 6 cut(s) 360, 417, 515, 597, 691, 2344
BspDI ATCGAT 1 cut(s) 1187
BspFNI CGCG 1 cut(s) 1810
BspHI TCATGA 2 cut(s) 1972, 2101
BspLI GGNNCC 3 cut(s) 82, 1307, 2860
BspMAI CTGCAG 2 cut(s) 166, 172
BspMI ACCTGC 2 cut(s) 1382, 2266
BspPI GGATC 5 cut(s) 1340, 1445, 1743, 3033, 3054
BspT104I TTCGAA 1 cut(s) 1443
BspTI CTTAAG 1 cut(s) 2378
BspTNI GGTCTC 3 cut(s) 54, 1582, 3257
BsrDI GCAATG 2 cut(s) 2037, 2280
BsrI ACTGG 7 cut(s) 28, 604, 838, 2465, 2582, 3156, 3179
BssECI CCNNGG 5 cut(s) 9, 847, 964, 2774, 2952
BssNI GRCGYC 1 cut(s) 2842
BssSI CACGAG 1 cut(s) 2294
BssT1I CCWWGG 4 cut(s) 9, 847, 2774, 2952
Bst2BI CACGAG 1 cut(s) 2294
Bst2UI CCWGG 2 cut(s) 1303, 3249
Bst6I CTCTTC 1 cut(s) 1863
BstACI GRCGYC 1 cut(s) 2842
BstAFI CTTAAG 1 cut(s) 2378
BstAPI GCANNNNNTGC 2 cut(s) 997, 2984
BstBI TTCGAA 1 cut(s) 1443
BstC8I GCNNGC 2 cut(s) 1334, 2980
BstDEI CTNAG 9 cut(s) 368, 425, 502, 566, 584, 699, 1002, 2352, 2705
BstDSI CCRYGG 2 cut(s) 964, 2952
BstENI CCTNNNNNAGG 2 cut(s) 2647, 2906
BstF5I GGATG 8 cut(s) 180, 336, 922, 1513, 1800, 2452, 2541, 2564
BstFNI CGCG 1 cut(s) 1810
BstH2I RGCGCY 1 cut(s) 2348
BstHHI GCGC 2 cut(s) 1810, 2347
BstMAI GTCTC 8 cut(s) 54, 131, 535, 1582, 2594, 2744, 3025, 3257
BstMCI CGRYCG 1 cut(s) 1130
BstMWI GCNNNNNNNGC 7 cut(s) 167, 176, 997, 1093, 1517, 2975, 2984
BstNI CCWGG 2 cut(s) 1303, 3249
BstNSI RCATGY 1 cut(s) 1557
BstSCI CCNGG 2 cut(s) 1301, 3247
BstSFI CTRYAG 4 cut(s) 162, 168, 877, 1455
BstSLI GKGCMC 1 cut(s) 1419
BstUI CGCG 1 cut(s) 1810
BstV1I GCAGC 6 cut(s) 148, 154, 176, 411, 1504, 2974
BstV2I GAAGAC 4 cut(s) 1214, 2253, 3143, 3151
BstX2I RGATCY 5 cut(s) 303, 2236, 2592, 3025, 3059
BstXI CCANNNNNNTGG 1 cut(s) 2023
BstYI RGATCY 5 cut(s) 303, 2236, 2592, 3025, 3059
Bsu15I ATCGAT 1 cut(s) 1187
Bsu36I CCTNAGG 2 cut(s) 425, 1002
BsuI GTATCC 1 cut(s) 1568
BsuRI GGCC 5 cut(s) 846, 1370, 1830, 2001, 2957
BsuTUI ATCGAT 1 cut(s) 1187
BtgI CCRYGG 2 cut(s) 964, 2952
BtrI CACGTC 1 cut(s) 615
BtsCI GGATG 8 cut(s) 180, 336, 922, 1513, 1800, 2452, 2541, 2564
BtsIMutI CAGTG 9 cut(s) 35, 365, 557, 592, 1146, 1521, 2334, 2589, 2699
BveI ACCTGC 2 cut(s) 1382, 2266
Cac8I GCNNGC 2 cut(s) 1334, 2980
CaiI CAGNNNCTG 2 cut(s) 68, 3185
CciI TCATGA 2 cut(s) 1972, 2101
CfoI GCGC 2 cut(s) 1810, 2347
Cfr13I GGNCC 3 cut(s) 1299, 2640, 3047
ClaI ATCGAT 1 cut(s) 1187
CseI GACGC 2 cut(s) 571, 3007
Csp6I GTAC 6 cut(s) 530, 765, 1124, 1773, 2183, 3053
CspCI CAANNNNNGTGG 2 cut(s) 1122, 1157
CviQI GTAC 6 cut(s) 530, 765, 1124, 1773, 2183, 3053
DdeI CTNAG 9 cut(s) 368, 425, 502, 566, 584, 699, 1002, 2352, 2705
DraI TTTAAA 2 cut(s) 1708, 2676
EaeI YGGCCR 2 cut(s) 1368, 1828
Eam1104I CTCTTC 1 cut(s) 1863
EarI CTCTTC 1 cut(s) 1863
Ecl136II GAGCTC 2 cut(s) 241, 1609
Eco130I CCWWGG 4 cut(s) 9, 847, 2774, 2952
Eco24I GRGCYC 2 cut(s) 243, 1611
Eco31I GGTCTC 3 cut(s) 54, 1582, 3257
Eco47I GGWCC 3 cut(s) 1299, 2640, 3047
Eco53kI GAGCTC 2 cut(s) 241, 1609
Eco57I CTGAAG 5 cut(s) 362, 1122, 2102, 2265, 3149
Eco81I CCTNAGG 2 cut(s) 425, 1002
Eco88I CYCGRG 1 cut(s) 2862
EcoICRI GAGCTC 2 cut(s) 241, 1609
EcoNI CCTNNNNNAGG 2 cut(s) 2647, 2906
EcoO109I RGGNCCY 1 cut(s) 2640
EcoRI GAATTC 2 cut(s) 23, 938
EcoRII CCWGG 2 cut(s) 1301, 3247
EcoT14I CCWWGG 4 cut(s) 9, 847, 2774, 2952
EcoT38I GRGCYC 2 cut(s) 243, 1611
ErhI CCWWGG 4 cut(s) 9, 847, 2774, 2952
Esp3I CGTCTC 3 cut(s) 131, 2594, 3025
FalI AAGNNNNNCTT 6 cut(s) 26, 58, 193, 225, 290, 322
FaqI GGGAC 5 cut(s) 584, 1337, 1688, 3045, 3161
FauI CCCGC 1 cut(s) 3001
FbaI TGATCA 5 cut(s) 1525, 2034, 2689, 2905, 3093
FblI GTMKAC 2 cut(s) 759, 1130
Fnu4HI GCNGC 9 cut(s) 6, 162, 165, 168, 356, 400, 1094, 1518, 2988
FokI GGATG 8 cut(s) 187, 323, 929, 1520, 1807, 2459, 2528, 2571
FriOI GRGCYC 2 cut(s) 243, 1611
Fsp4HI GCNGC 9 cut(s) 6, 162, 165, 168, 356, 400, 1094, 1518, 2988
FspBI CTAG 9 cut(s) 768, 848, 1791, 2369, 2715, 2751, 2924, 3207, 3213
GlaI GCGC 2 cut(s) 1809, 2346
GluI GCNGC 9 cut(s) 6, 162, 165, 168, 356, 400, 1094, 1518, 2988
GsuI CTGGAG 2 cut(s) 1140, 1312
HaeII RGCGCY 1 cut(s) 2348
HaeIII GGCC 5 cut(s) 846, 1370, 1830, 2001, 2957
HapII CCGG 2 cut(s) 1353, 1505
HgaI GACGC 2 cut(s) 571, 3007
HhaI GCGC 2 cut(s) 1810, 2347
Hin1I GRCGYC 1 cut(s) 2842
Hin6I GCGC 2 cut(s) 1808, 2345
HinP1I GCGC 2 cut(s) 1808, 2345
HincII GTYRAC 1 cut(s) 1131
HindII GTYRAC 1 cut(s) 1131
HindIII AAGCTT 1 cut(s) 2980
HpaII CCGG 2 cut(s) 1353, 1505
HphI GGTGA 9 cut(s) 499, 1254, 1355, 1733, 1934, 2140, 2453, 2493, 2699
HpyAV CCTTC 6 cut(s) 330, 1240, 1712, 1919, 2261, 3149
HpyCH4IV ACGT 4 cut(s) 410, 614, 2842, 3174
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 176, 997, 1093, 1517, 2975, 2984
HpyF3I CTNAG 9 cut(s) 368, 425, 502, 566, 584, 699, 1002, 2352, 2705
HpySE526I ACGT 4 cut(s) 410, 614, 2842, 3174
Hsp92I GRCGYC 1 cut(s) 2842
HspAI GCGC 2 cut(s) 1808, 2345
Ksp22I TGATCA 5 cut(s) 1525, 2034, 2689, 2905, 3093
LmnI GCTCC 2 cut(s) 238, 289
Lsp1109I GCAGC 6 cut(s) 148, 154, 176, 411, 1504, 2974
LweI GCATC 4 cut(s) 1498, 1996, 2560, 2956
MaeI CTAG 9 cut(s) 768, 848, 1791, 2369, 2715, 2751, 2924, 3207, 3213
MaeII ACGT 4 cut(s) 410, 614, 2842, 3174
MfeI CAATTG 3 cut(s) 2010, 2730, 3189
MflI RGATCY 5 cut(s) 303, 2236, 2592, 3025, 3059
MhlI GDGCHC 4 cut(s) 243, 1419, 1611, 2368
MlsI TGGCCA 1 cut(s) 1830
MluNI TGGCCA 1 cut(s) 1830
MlyI GAGTC 6 cut(s) 74, 846, 1319, 1757, 2014, 2492
MmeI TCCRAC 4 cut(s) 894, 1455, 1905, 1971
Mox20I TGGCCA 1 cut(s) 1830
MroXI GAANNNNTTC 3 cut(s) 38, 1728, 2250
MscI TGGCCA 1 cut(s) 1830
MslI CAYNNNNRTG 3 cut(s) 389, 1412, 2685
Msp20I TGGCCA 1 cut(s) 1830
MspA1I CMGCKG 2 cut(s) 167, 3008
MspCI CTTAAG 1 cut(s) 2378
MspI CCGG 2 cut(s) 1353, 1505
MspR9I CCNGG 2 cut(s) 1303, 3249
MunI CAATTG 3 cut(s) 2010, 2730, 3189
Mva1269I GAATGC 1 cut(s) 2709
MvaI CCWGG 2 cut(s) 1303, 3249
MvnI CGCG 1 cut(s) 1810
MwoI GCNNNNNNNGC 7 cut(s) 167, 176, 997, 1093, 1517, 2975, 2984
NcoI CCATGG 1 cut(s) 2952
NlaIV GGNNCC 3 cut(s) 82, 1307, 2860
NmeAIII GCCGAG 1 cut(s) 1630
NmuCI GTSAC 7 cut(s) 587, 1549, 1783, 2128, 2222, 2971, 3121
NspI RCATGY 1 cut(s) 1557
NspV TTCGAA 1 cut(s) 1443
OliI CACNNNNGTG 1 cut(s) 1412
PagI TCATGA 2 cut(s) 1972, 2101
PaqCI CACCTGC 1 cut(s) 1382
PciI ACATGT 1 cut(s) 1553
PctI GAATGC 1 cut(s) 2709
PdmI GAANNNNTTC 3 cut(s) 38, 1728, 2250
PfeI GAWTC 9 cut(s) 142, 267, 316, 609, 979, 1981, 2164, 2529, 2896
PfoI TCCNGGA 1 cut(s) 1301
PkrI GCNGC 9 cut(s) 7, 163, 166, 169, 357, 401, 1095, 1519, 2989
PleI GAGTC 6 cut(s) 73, 846, 1319, 1757, 2014, 2492
PpsI GAGTC 6 cut(s) 73, 846, 1319, 1757, 2014, 2492
PpuMI RGGWCCY 1 cut(s) 2640
PscI ACATGT 1 cut(s) 1553
PsiI TTATAA 3 cut(s) 2067, 2432, 2456
Psp124BI GAGCTC 2 cut(s) 243, 1611
Psp5II RGGWCCY 1 cut(s) 2640
Psp6I CCWGG 2 cut(s) 1301, 3247
PspGI CCWGG 2 cut(s) 1301, 3247
PspN4I GGNNCC 3 cut(s) 82, 1307, 2860
PspPI GGNCC 3 cut(s) 1299, 2640, 3047
PspPPI RGGWCCY 1 cut(s) 2640
PsrI GAACNNNNNNTAC 2 cut(s) 2104, 2136
PstI CTGCAG 2 cut(s) 166, 172
PstNI CAGNNNCTG 2 cut(s) 68, 3185
PsuI RGATCY 5 cut(s) 303, 2236, 2592, 3025, 3059
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 6 cut(s) 531, 766, 1125, 1774, 2184, 3054
RsaNI GTAC 6 cut(s) 530, 765, 1124, 1773, 2183, 3053
RseI CAYNNNNRTG 3 cut(s) 389, 1412, 2685
SacI GAGCTC 2 cut(s) 243, 1611
SalI GTCGAC 1 cut(s) 1129
SatI GCNGC 9 cut(s) 6, 162, 165, 168, 356, 400, 1094, 1518, 2988
Sau96I GGNCC 3 cut(s) 1299, 2640, 3047
SchI GAGTC 6 cut(s) 74, 846, 1319, 1757, 2014, 2492
ScrFI CCNGG 2 cut(s) 1303, 3249
SduI GDGCHC 4 cut(s) 243, 1419, 1611, 2368
SfaNI GCATC 4 cut(s) 1498, 1996, 2560, 2956
SfcI CTRYAG 4 cut(s) 162, 168, 877, 1455
SfuI TTCGAA 1 cut(s) 1443
SinI GGWCC 3 cut(s) 1299, 2640, 3047
SmiMI CAYNNNNRTG 3 cut(s) 389, 1412, 2685
SmlI CTYRAG 2 cut(s) 43, 2378
SmoI CTYRAG 2 cut(s) 43, 2378
SpeI ACTAGT 1 cut(s) 767
SsiI CCGC 5 cut(s) 355, 1093, 1920, 3008, 3042
SspI AATATT 2 cut(s) 2199, 3071
SspMI CTAG 9 cut(s) 768, 848, 1791, 2369, 2715, 2751, 2924, 3207, 3213
SstI GAGCTC 2 cut(s) 243, 1611
StyD4I CCNGG 2 cut(s) 1301, 3247
StyI CCWWGG 4 cut(s) 9, 847, 2774, 2952
TaiI ACGT 4 cut(s) 413, 617, 2845, 3177
TaqI TCGA 7 cut(s) 453, 1056, 1130, 1187, 1443, 2324, 3200
TauI GCSGC 2 cut(s) 358, 1096
TfiI GAWTC 9 cut(s) 142, 267, 316, 609, 979, 1981, 2164, 2529, 2896
TscAI CASTG 9 cut(s) 35, 372, 564, 592, 1146, 1528, 2334, 2589, 2699
TseFI GTSAC 7 cut(s) 587, 1549, 1783, 2128, 2222, 2971, 3121
TseI GCWGC 7 cut(s) 5, 161, 164, 167, 399, 1517, 2987
Tsp45I GTSAC 7 cut(s) 587, 1549, 1783, 2128, 2222, 2971, 3121
TspDTI ATGAA 7 cut(s) 325, 362, 1920, 1961, 2112, 2118, 2591
TspGWI ACGGA 2 cut(s) 1764, 1814
TspRI CASTG 9 cut(s) 35, 372, 564, 592, 1146, 1528, 2334, 2589, 2699
Vha464I CTTAAG 1 cut(s) 2378
VneI GTGCAC 1 cut(s) 1415
VpaK11BI GGWCC 3 cut(s) 1299, 2640, 3047
XagI CCTNNNNNAGG 2 cut(s) 2647, 2906
XceI RCATGY 1 cut(s) 1557
XmaJI CCTAGG 1 cut(s) 847
XmiI GTMKAC 2 cut(s) 759, 1130
XmnI GAANNNNTTC 3 cut(s) 38, 1728, 2250
XspI CTAG 9 cut(s) 768, 848, 1791, 2369, 2715, 2751, 2924, 3207, 3213
ZraI GACGTC 1 cut(s) 2843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.