Rh7AG408200

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000202
Physical Location & Seq
Forward (+)
54399281 .. 54400543
1263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG408200.1

Sequence Viewer

Length: 1263 bp
ATGGCATTAGATGTTGCTGAATTGCTGTGGAGCAAGTTCTTGAAGTCTCTGGCAGAGCTAGACTCTGAATCTAATTCAGGTTCTATTACCATCTCTGTCTTCAAATCATCAATAAAAGCTCTCGTGTCTGATTTCGAAGCAATTAAAGACGTTGTGAATGAGAGAGACATAATGCCGGAACCTTATTCCTACTCTGAAACTATCAATCTGCTCTACAAACTCAACGATGCATTGGCTGACTGCCGAGTCTTCACACACGAGTGTATGGAGCTTAATAATGTGATCTCACGCTTCAACTTTCGAAGATTTACTTTTGTCGCAAAGGTTAAGAGTCGGTTGACCAAATTAAAGCAGGAGCTTGAGCACTTGCCGCAACAACCAATTGGCAATCCTCAGACAAAGACCGAATTCACCAGATCCGGACCGAAAGTGCCTTCAGCCAAAGTTTTCGGATTTGATGAGCAATTAAAGGAGATTAATGAGGAGTTGCTACAAGCAGGGGGTTCCAGTTCTAGCAGTGCTGGTGAACTGAGGGCAATTGGGGTAGTTGGAATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTGGAGAGCAAGGAAGTGAAGCAGAAGTTTAATCATATACTTTGGTTACCCTTTTCGTTGAGTGAGGAAGAAGAACAATATAGTAAGTTCAGCTTTGAGACACGTATCTTTGATAGTAATGGCCAAATCATAGAGGTGAGGATTGTTGGCCTTGATAAGCTCCTGGAAAGGCTAAGCCGCCTGCTGTTATCGGATAAATGCTATTTGATTGTGTTGGATGATGTGTGTCATCGGCACATTAACATTATGGAACGCTTATGCAGCGGATTGCCCAAGCATAATGGTGGTGTTGTCATTGTCACTACTAGATTGAAGGAAGTGGCTCAAAAGCTGGGGAAACAACATAGATTGCATCTGGTTCATGTTAAGCCTCTGGACAGAGAGATTTGCGGGCGTATATTTGAGGAGCAGGCTTATTCCATTAGAAAAAGTTCAAACCTCTCACATGATGAGGCTACGAGAAAGATGGAGGAACTCAAGGATCAGTGCCATGGTCTACCGTTGGTTGCGAAGACGATAGCAAATGCTTTTGCAGTGGGATTTGGAGGCGACGGATCTGAAGAGAGAGATGGAGATCACGGGGAGGAAGTAGGAACTTTTGAAGAATCAGTTGAAGACGACCAGGGTTCAAGAATTGCTGACCTGACCCATCATGTTCAAGAATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

420

Amino Acids

47.16

Weight (kDa)

5.76

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 175 - 336 3.8e-21 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 1087
AccIII TCCGGA 1 cut(s) 419
AciI CCGC 4 cut(s) 371, 769, 855, 981
AclWI GGATC 3 cut(s) 411, 1080, 1153
AcoI YGGCCR 1 cut(s) 712
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 2 cut(s) 420, 1170
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 9 cut(s) 43, 103, 295, 904, 1026, 1193, 1205, 1221, 1250
AjnI CCWGG 3 cut(s) 576, 753, 1212
AleI CACNNNNGTG 1 cut(s) 259
AluBI AGCT 7 cut(s) 58, 119, 271, 358, 684, 751, 922
AluI AGCT 7 cut(s) 58, 119, 271, 358, 684, 751, 922
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 3 cut(s) 51, 159, 683
AlwI GGATC 3 cut(s) 411, 1080, 1153
Aor13HI TCCGGA 1 cut(s) 419
AoxI GGCC 2 cut(s) 712, 739
ApeKI GCWGC 1 cut(s) 852
ApoI RAATTY 1 cut(s) 407
AseI ATTAAT 1 cut(s) 477
Asp700I GAANNNNTTC 1 cut(s) 1021
AspS9I GGNCC 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 403, 536, 739
AsuII TTCGAA 2 cut(s) 135, 301
AvaII GGWCC 1 cut(s) 422
BalI TGGCCA 1 cut(s) 714
BauI CACGAG 2 cut(s) 122, 257
BbsI GAAGAC 4 cut(s) 91, 241, 1109, 1212
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 864
BccI CCATC 4 cut(s) 98, 1051, 1154, 1248
BciT130I CCWGG 3 cut(s) 578, 755, 1214
BcoDI GTCTC 3 cut(s) 51, 159, 683
BfaI CTAG 3 cut(s) 59, 513, 897
BisI GCNGC 3 cut(s) 371, 769, 853
BlpI GCTNAGC 1 cut(s) 764
BlsI GCNGC 3 cut(s) 372, 770, 854
Bme1390I CCNGG 3 cut(s) 578, 755, 1214
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 180, 505
BmrFI CCNGG 3 cut(s) 578, 755, 1214
BmsI GCATC 2 cut(s) 217, 952
BpiI GAAGAC 4 cut(s) 91, 241, 1109, 1212
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BpmI CTGGAG 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 764
Bpu14I TTCGAA 2 cut(s) 135, 301
BpuEI CTTGAG 2 cut(s) 380, 1052
BsaAI YACGTR 1 cut(s) 695
BsaBI GATNNNNATC 1 cut(s) 1164
BsaJI CCNNGG 3 cut(s) 576, 1081, 1213
BsaWI WCCGGW 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 1164
BseAI TCCGGA 1 cut(s) 419
BseBI CCWGG 3 cut(s) 578, 755, 1214
BseDI CCNNGG 3 cut(s) 576, 1081, 1213
BseGI GGATG 1 cut(s) 814
BseJI GATNNNNATC 1 cut(s) 1164
BseMII CTCAG 2 cut(s) 407, 521
BseNI ACTGG 1 cut(s) 507
BseRI GAGGAG 2 cut(s) 497, 1010
BseXI GCAGC 1 cut(s) 864
BseYI CCCAGC 1 cut(s) 922
BshFI GGCC 2 cut(s) 714, 741
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 2 cut(s) 176, 420
BsmAI GTCTC 3 cut(s) 51, 159, 683
BsnI GGCC 2 cut(s) 714, 741
Bsp119I TTCGAA 2 cut(s) 135, 301
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 419
Bsp143I GATC 5 cut(s) 282, 416, 1072, 1145, 1165
Bsp1720I GCTNAGC 1 cut(s) 764
Bsp19I CCATGG 1 cut(s) 1081
BspACI CCGC 4 cut(s) 371, 769, 855, 981
BspANI GGCC 2 cut(s) 714, 741
BspCNI CTCAG 2 cut(s) 406, 522
BspEI TCCGGA 1 cut(s) 419
BspLI GGNNCC 2 cut(s) 180, 505
BspPI GGATC 3 cut(s) 411, 1080, 1153
BspT104I TTCGAA 2 cut(s) 135, 301
BsrI ACTGG 1 cut(s) 507
BssECI CCNNGG 3 cut(s) 576, 1081, 1213
BssMI GATC 5 cut(s) 282, 416, 1072, 1145, 1165
BssSI CACGAG 2 cut(s) 122, 257
BssT1I CCWWGG 1 cut(s) 1081
Bst2BI CACGAG 2 cut(s) 122, 257
Bst2UI CCWGG 3 cut(s) 578, 755, 1214
Bst4CI ACNGT 1 cut(s) 1092
Bst6I CTCTTC 1 cut(s) 1146
BstBAI YACGTR 1 cut(s) 695
BstBI TTCGAA 2 cut(s) 135, 301
BstC8I GCNNGC 3 cut(s) 773, 983, 1002
BstDEI CTNAG 3 cut(s) 393, 530, 764
BstDSI CCRYGG 1 cut(s) 1081
BstEII GGTNACC 1 cut(s) 636
BstF5I GGATG 1 cut(s) 814
BstKTI GATC 5 cut(s) 285, 419, 1075, 1148, 1168
BstMAI GTCTC 3 cut(s) 51, 159, 683
BstMBI GATC 5 cut(s) 282, 416, 1072, 1145, 1165
BstMWI GCNNNNNNNGC 2 cut(s) 370, 852
BstNI CCWGG 3 cut(s) 578, 755, 1214
BstPI GGTNACC 1 cut(s) 636
BstSCI CCNGG 3 cut(s) 576, 753, 1212
BstV1I GCAGC 1 cut(s) 864
BstV2I GAAGAC 4 cut(s) 91, 241, 1109, 1212
BstX2I RGATCY 2 cut(s) 416, 1145
BstYI RGATCY 2 cut(s) 416, 1145
BsuRI GGCC 2 cut(s) 714, 741
BtgI CCRYGG 1 cut(s) 1081
BtsCI GGATG 1 cut(s) 814
BtsI GCAGTG 2 cut(s) 523, 1131
BtsIMutI CAGTG 3 cut(s) 523, 1082, 1131
Cac8I GCNNGC 3 cut(s) 773, 983, 1002
Cfr13I GGNCC 1 cut(s) 422
CpoI CGGWCCG 1 cut(s) 422
CspI CGGWCCG 1 cut(s) 422
CviAII CATG 4 cut(s) 953, 1037, 1082, 1244
DdeI CTNAG 3 cut(s) 393, 530, 764
DpnI GATC 5 cut(s) 284, 418, 1074, 1147, 1167
DpnII GATC 5 cut(s) 282, 416, 1072, 1145, 1165
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 712
Eam1104I CTCTTC 1 cut(s) 1146
EarI CTCTTC 1 cut(s) 1146
Eco130I CCWWGG 1 cut(s) 1081
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 2 cut(s) 420, 1170
Eco91I GGTNACC 1 cut(s) 636
EcoO65I GGTNACC 1 cut(s) 636
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 3 cut(s) 576, 753, 1212
EcoT14I CCWWGG 1 cut(s) 1081
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 1081
FaeI CATG 4 cut(s) 956, 1040, 1085, 1247
FalI AAGNNNNNCTT 4 cut(s) 295, 327, 668, 700
FatI CATG 4 cut(s) 952, 1036, 1081, 1243
FauI CCCGC 1 cut(s) 974
FblI GTMKAC 1 cut(s) 1087
Fnu4HI GCNGC 3 cut(s) 371, 769, 853
FokI GGATG 1 cut(s) 821
Fsp4HI GCNGC 3 cut(s) 371, 769, 853
FspBI CTAG 3 cut(s) 59, 513, 897
GluI GCNGC 3 cut(s) 371, 769, 853
GsaI CCCAGC 1 cut(s) 926
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 2 cut(s) 714, 741
HapII CCGG 2 cut(s) 176, 420
Hin1II CATG 4 cut(s) 956, 1040, 1085, 1247
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HinfI GANTC 6 cut(s) 62, 68, 246, 331, 1196, 1253
HpaII CCGG 2 cut(s) 176, 420
HphI GGTGA 3 cut(s) 403, 536, 739
Hpy166II GTNNAC 3 cut(s) 339, 527, 1088
Hpy188I TCNGA 8 cut(s) 67, 130, 196, 396, 452, 585, 784, 1150
Hpy188III TCNNGA 6 cut(s) 40, 420, 965, 1221, 1250, 1260
Hpy8I GTNNAC 3 cut(s) 339, 527, 1088
Hpy99I CGWCG 1 cut(s) 1145
HpyAV CCTTC 3 cut(s) 444, 580, 898
HpyCH4III ACNGT 1 cut(s) 1092
HpyCH4IV ACGT 2 cut(s) 150, 694
HpyCH4V TGCA 4 cut(s) 230, 852, 943, 1124
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 852
HpyF3I CTNAG 3 cut(s) 393, 530, 764
HpySE526I ACGT 2 cut(s) 150, 694
Hsp92II CATG 4 cut(s) 956, 1040, 1085, 1247
Kpn2I TCCGGA 1 cut(s) 419
Kzo9I GATC 5 cut(s) 282, 416, 1072, 1145, 1165
LmnI GCTCC 5 cut(s) 30, 268, 355, 756, 997
Lsp1109I GCAGC 1 cut(s) 864
LweI GCATC 2 cut(s) 217, 952
MaeI CTAG 3 cut(s) 59, 513, 897
MaeII ACGT 2 cut(s) 150, 694
MaeIII GTNAC 2 cut(s) 636, 889
MalI GATC 5 cut(s) 284, 418, 1074, 1147, 1167
MboI GATC 5 cut(s) 282, 416, 1072, 1145, 1165
MfeI CAATTG 2 cut(s) 381, 537
MflI RGATCY 2 cut(s) 416, 1145
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 714
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 3 cut(s) 56, 255, 340
MmeI TCCRAC 2 cut(s) 529, 786
Mox20I TGGCCA 1 cut(s) 714
Mph1103I ATGCAT 1 cut(s) 232
MroI TCCGGA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 1021
MscI TGGCCA 1 cut(s) 714
MseI TTAA 9 cut(s) 144, 273, 327, 347, 467, 477, 621, 831, 957
MslI CAYNNNNRTG 3 cut(s) 259, 725, 873
Msp20I TGGCCA 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 2 cut(s) 176, 420
MspR9I CCNGG 3 cut(s) 578, 755, 1214
MunI CAATTG 2 cut(s) 381, 537
MvaI CCWGG 3 cut(s) 578, 755, 1214
MwoI GCNNNNNNNGC 2 cut(s) 370, 852
NcoI CCATGG 1 cut(s) 1081
NdeII GATC 5 cut(s) 282, 416, 1072, 1145, 1165
NlaIII CATG 4 cut(s) 956, 1040, 1085, 1247
NlaIV GGNNCC 2 cut(s) 180, 505
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 889
NsiI ATGCAT 1 cut(s) 232
NspV TTCGAA 2 cut(s) 135, 301
OliI CACNNNNGTG 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 1021
PfeI GAWTC 3 cut(s) 68, 1196, 1253
PfoI TCCNGGA 1 cut(s) 753
PkrI GCNGC 3 cut(s) 372, 770, 854
PleI GAGTC 3 cut(s) 56, 254, 339
PpsI GAGTC 3 cut(s) 56, 254, 339
Ppu21I YACGTR 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 477
Psp6I CCWGG 3 cut(s) 576, 753, 1212
PspEI GGTNACC 1 cut(s) 636
PspFI CCCAGC 1 cut(s) 922
PspGI CCWGG 3 cut(s) 576, 753, 1212
PspN4I GGNNCC 2 cut(s) 180, 505
PspPI GGNCC 1 cut(s) 422
PsuI RGATCY 2 cut(s) 416, 1145
RseI CAYNNNNRTG 3 cut(s) 259, 725, 873
Rsr2I CGGWCCG 1 cut(s) 422
RsrII CGGWCCG 1 cut(s) 422
SaqAI TTAA 9 cut(s) 144, 273, 327, 347, 467, 477, 621, 831, 957
SatI GCNGC 3 cut(s) 371, 769, 853
Sau3AI GATC 5 cut(s) 282, 416, 1072, 1145, 1165
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 3 cut(s) 56, 255, 340
ScrFI CCNGG 3 cut(s) 578, 755, 1214
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 2 cut(s) 217, 952
SfuI TTCGAA 2 cut(s) 135, 301
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 3 cut(s) 259, 725, 873
SmlI CTYRAG 2 cut(s) 359, 1067
SmoI CTYRAG 2 cut(s) 359, 1067
SsiI CCGC 4 cut(s) 371, 769, 855, 981
SspMI CTAG 3 cut(s) 59, 513, 897
StyD4I CCNGG 3 cut(s) 576, 753, 1212
StyI CCWWGG 1 cut(s) 1081
TaaI ACNGT 1 cut(s) 1092
TaiI ACGT 2 cut(s) 153, 697
TaqI TCGA 2 cut(s) 135, 301
TaqII GACCGA 2 cut(s) 419, 439
TauI GCSGC 2 cut(s) 373, 771
TfiI GAWTC 3 cut(s) 68, 1196, 1253
Tru1I TTAA 9 cut(s) 144, 273, 327, 347, 467, 477, 621, 831, 957
Tru9I TTAA 9 cut(s) 144, 273, 327, 347, 467, 477, 621, 831, 957
TscAI CASTG 3 cut(s) 523, 1082, 1131
TseFI GTSAC 1 cut(s) 889
TseI GCWGC 1 cut(s) 852
Tsp45I GTSAC 1 cut(s) 889
TspDTI ATGAA 1 cut(s) 941
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 3 cut(s) 523, 1082, 1131
VpaK11BI GGWCC 1 cut(s) 422
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 1087
XmnI GAANNNNTTC 1 cut(s) 1021
XspI CTAG 3 cut(s) 59, 513, 897
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.