Rh2BG251200

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000202
Physical Location & Seq
Reverse (-)
25848259 .. 25850152
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG251200.1

Sequence Viewer

Length: 1110 bp
ATGACATTGGATGTTACTGAATTCCTCTGGAACAAGTTCTTGAAGTCTCTGGCAGAGCTAGAGTCTGAATCTAGTTCAGGTTTTGTTACCATCTCTTTCTTCAACTCATCATTGAAAAATCTCGTGTCTAACTTCAAATCAATCAGAGATGTTGTGATTGAGAGAGATCTAATTTCGGAACGTTATACCTACTCTAAAACTATCGATGTGCTCTACGAGCTCAACGATGCGTTGGCCGAGTGCCGAATCATCTCACATGAGTGCAAAGAAATTAAGAATAAGTTGTCAAGCCTCAACCCTCGAAGAGTTATTTCTCTCACCACGATCAAGGAAAAGCTGAAAACAATTGAGGATAAGCTTTACAATTTGGCGTCGTCACCGCAGGAAACTGGCCTTCCCCTGGTGATGGCTGGACCTATGCTTACACCAGAAGTTTTCGGATTTGAGAAGCTATCAGAGGAGATCGAGGGTTTGTTACTTGGGGGTTCTAGTTCTAGCAGTGTGAGATTTACGGAAATAGGGTTAGTTGGCATGGCTGGCGTGGGTAAGACCACTCTGGTACAGAAGGTTTTGGCGAGCAAGGAAGTGAAAAACAAGTTTGATCTTATACTTTGGTTACCCTTTTCGGGTATAACGGAAAGAGAACAACAGTATAGTAGGTTCACTTTTGAGACCTGCATCCTAAATAATTCGGGCGAAACAGAAGCGAAGATTGTTGGCCTTGATAGTCTCCTGGAAAAGCTGGGCCAACTGTTATCGGATAAAAGCTATTTGATTGTGTTGGATGATGTGTGTTTTCAGCTGATTAACATTATGGAACGCCTAGTCAGTGGATTGCCCAAGGGTAGTGGTGGTGCTGTCATCGTCACTACTAGACTGAAGGAAGTGGCTCAAAAGCTTGGGGAACACCATCACTTCGTTCGTGTTAAGCCTCTGCCCAGAAAAAGTTGCTCGAGTATATTTGAGGAGACCATTGAAAATGAGAAGAAATTGTCAAGCATGTCACCTGGTGAAACTCTGGAAAAGATTAAGGAGAAGATGAAGGATCAATGTCATGGTCTGCCGTTGGTTGCGAAGGCACTGGCACAGATCATTCCTGAAACTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

369

Amino Acids

41.16

Weight (kDa)

6.09

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 172 - 326 4.2e-24 NB-ARC domain
NACHT PF05729 175 - 327 3.3e-07 NACHT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 683
AciI CCGC 1 cut(s) 380
AclI AACGTT 1 cut(s) 181
AclWI GGATC 1 cut(s) 1053
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 371
AdeI CACNNNGTG 1 cut(s) 1010
AfaI GTAC 1 cut(s) 561
AfiI CCNNNNNNNGG 3 cut(s) 400, 406, 626
AgsI TTSAA 5 cut(s) 43, 103, 115, 136, 977
AjnI CCWGG 3 cut(s) 399, 732, 1006
AleI CACNNNNGTG 1 cut(s) 259
AluBI AGCT 9 cut(s) 58, 220, 337, 358, 451, 742, 768, 802, 898
AluI AGCT 9 cut(s) 58, 220, 337, 358, 451, 742, 768, 802, 898
Alw21I GWGCWC 2 cut(s) 213, 222
Alw26I GTCTC 4 cut(s) 51, 665, 734, 962
AlwI GGATC 1 cut(s) 1053
Ama87I CYCGRG 1 cut(s) 952
AoxI GGCC 4 cut(s) 234, 391, 718, 745
ApoI RAATTY 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 413, 745
AsuHPI GGTGA 5 cut(s) 310, 369, 415, 996, 1022
AvaI CYCGRG 1 cut(s) 952
AvaII GGWCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 222
BauI CACGAG 1 cut(s) 122
Bbv12I GWGCWC 2 cut(s) 213, 222
BccI CCATC 3 cut(s) 98, 400, 918
BceAI ACGGC 1 cut(s) 1048
BciT130I CCWGG 3 cut(s) 401, 734, 1008
BcoDI GTCTC 4 cut(s) 51, 665, 734, 962
BfaI CTAG 6 cut(s) 59, 72, 489, 495, 824, 873
BfuAI ACCTGC 1 cut(s) 683
BglII AGATCT 1 cut(s) 166
Bme1390I CCNGG 3 cut(s) 401, 734, 1008
Bme18I GGWCC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 952
BmgT120I GGNCC 2 cut(s) 413, 745
BmrFI CCNGG 3 cut(s) 401, 734, 1008
BmsI GCATC 2 cut(s) 217, 687
Bsa29I ATCGAT 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 371
BsaI GGTCTC 2 cut(s) 665, 962
BsaJI CCNNGG 2 cut(s) 399, 840
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 406, 626
Bse1I ACTGG 2 cut(s) 394, 1086
BseBI CCWGG 3 cut(s) 401, 734, 1008
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 399, 840
BseGI GGATG 3 cut(s) 16, 678, 790
BseLI CCNNNNNNNGG 3 cut(s) 400, 406, 626
BseNI ACTGG 2 cut(s) 394, 1086
BseRI GAGGAG 2 cut(s) 473, 980
BseYI CCCAGC 1 cut(s) 742
BshFI GGCC 4 cut(s) 236, 393, 720, 747
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 2 cut(s) 213, 222
BsiHKCI CYCGRG 1 cut(s) 952
BslI CCNNNNNNNGG 3 cut(s) 400, 406, 626
BsmAI GTCTC 4 cut(s) 51, 665, 734, 962
BsnI GGCC 4 cut(s) 236, 393, 720, 747
Bso31I GGTCTC 2 cut(s) 665, 962
BsoBI CYCGRG 1 cut(s) 952
Bsp1286I GDGCHC 2 cut(s) 213, 222
Bsp143I GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
BspACI CCGC 1 cut(s) 380
BspANI GGCC 4 cut(s) 236, 393, 720, 747
BspDI ATCGAT 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 683
BspPI GGATC 1 cut(s) 1053
BspTNI GGTCTC 2 cut(s) 665, 962
BsrI ACTGG 2 cut(s) 394, 1086
BssECI CCNNGG 2 cut(s) 399, 840
BssMI GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
BssNI GRCGYC 1 cut(s) 371
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 840
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 401, 734, 1008
Bst4CI ACNGT 2 cut(s) 651, 753
Bst6I CTCTTC 1 cut(s) 298
BstACI GRCGYC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 538, 577
BstEII GGTNACC 1 cut(s) 615
BstF5I GGATG 3 cut(s) 16, 678, 790
BstKTI GATC 6 cut(s) 169, 327, 465, 604, 1048, 1092
BstMAI GTCTC 4 cut(s) 51, 665, 734, 962
BstMBI GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
BstMWI GCNNNNNNNGC 2 cut(s) 217, 537
BstNI CCWGG 3 cut(s) 401, 734, 1008
BstNSI RCATGY 1 cut(s) 1003
BstPI GGTNACC 1 cut(s) 615
BstSCI CCNGG 3 cut(s) 399, 732, 1006
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 4 cut(s) 236, 393, 720, 747
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 3 cut(s) 16, 678, 790
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 3 cut(s) 505, 835, 1079
BveI ACCTGC 1 cut(s) 683
Cac8I GCNNGC 2 cut(s) 538, 577
Cfr13I GGNCC 2 cut(s) 413, 745
ClaI ATCGAT 1 cut(s) 204
CseI GACGC 1 cut(s) 360
CsiI ACCWGGT 1 cut(s) 1006
Csp6I GTAC 1 cut(s) 560
CspCI CAANNNNNGTGG 2 cut(s) 829, 864
CviAII CATG 4 cut(s) 257, 532, 1000, 1055
CviQI GTAC 1 cut(s) 560
DpnI GATC 6 cut(s) 168, 326, 464, 603, 1047, 1091
DpnII GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
DraIII CACNNNGTG 1 cut(s) 1010
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Ecl136II GAGCTC 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 840
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 2 cut(s) 665, 962
Eco47I GGWCC 1 cut(s) 413
Eco53kI GAGCTC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 899
Eco88I CYCGRG 1 cut(s) 952
Eco91I GGTNACC 1 cut(s) 615
EcoICRI GAGCTC 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 3 cut(s) 399, 732, 1006
EcoT14I CCWWGG 1 cut(s) 840
EcoT38I GRGCYC 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 4 cut(s) 260, 535, 1003, 1058
FatI CATG 4 cut(s) 256, 531, 999, 1054
FokI GGATG 3 cut(s) 23, 665, 797
FriOI GRGCYC 1 cut(s) 222
FspBI CTAG 6 cut(s) 59, 72, 489, 495, 824, 873
GsaI CCCAGC 1 cut(s) 746
HaeIII GGCC 4 cut(s) 236, 393, 720, 747
HgaI GACGC 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 4 cut(s) 260, 535, 1003, 1058
HindIII AAGCTT 2 cut(s) 356, 896
HinfI GANTC 3 cut(s) 62, 68, 246
HphI GGTGA 5 cut(s) 310, 369, 415, 996, 1022
Hpy166II GTNNAC 1 cut(s) 663
Hpy188I TCNGA 6 cut(s) 67, 146, 178, 440, 457, 760
Hpy188III TCNNGA 4 cut(s) 28, 40, 1019, 1097
Hpy8I GTNNAC 1 cut(s) 663
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 5 cut(s) 404, 559, 874, 1036, 1069
HpyCH4III ACNGT 2 cut(s) 651, 753
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 2 cut(s) 264, 678
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 537
HpySE526I ACGT 1 cut(s) 181
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 4 cut(s) 260, 535, 1003, 1058
Kzo9I GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
LweI GCATC 2 cut(s) 217, 687
MabI ACCWGGT 1 cut(s) 1006
MaeI CTAG 6 cut(s) 59, 72, 489, 495, 824, 873
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 7 cut(s) 13, 85, 375, 474, 615, 865, 1002
MalI GATC 6 cut(s) 168, 326, 464, 603, 1047, 1091
MboI GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
MboII GAAGA 5 cut(s) 91, 315, 721, 997, 1048
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 1 cut(s) 166
MhlI GDGCHC 2 cut(s) 213, 222
MluCI AATT 7 cut(s) 20, 171, 270, 345, 364, 688, 989
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 762
MnlI CCTC 8 cut(s) 35, 302, 309, 343, 451, 460, 942, 958
MroXI GAANNNNTTC 1 cut(s) 35
MseI TTAA 4 cut(s) 273, 807, 927, 1029
MslI CAYNNNNRTG 1 cut(s) 259
MspA1I CMGCKG 1 cut(s) 802
MspR9I CCNGG 3 cut(s) 401, 734, 1008
MunI CAATTG 1 cut(s) 345
MvaI CCWGG 3 cut(s) 401, 734, 1008
MwoI GCNNNNNNNGC 2 cut(s) 217, 537
NdeII GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
NlaIII CATG 4 cut(s) 260, 535, 1003, 1058
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 375, 865, 1002
NspI RCATGY 1 cut(s) 1003
OliI CACNNNNGTG 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 952
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 2 cut(s) 68, 246
PfoI TCCNGGA 1 cut(s) 732
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 399, 732, 1006
PspEI GGTNACC 1 cut(s) 615
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 3 cut(s) 399, 732, 1006
PspPI GGNCC 2 cut(s) 413, 745
PspXI VCTCGAGB 1 cut(s) 952
PsrI GAACNNNNNNTAC 2 cut(s) 644, 676
PsuI RGATCY 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 802
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 259
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 4 cut(s) 273, 807, 927, 1029
Sau3AI GATC 6 cut(s) 166, 324, 462, 601, 1045, 1089
Sau96I GGNCC 2 cut(s) 413, 745
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 401, 734, 1008
SduI GDGCHC 2 cut(s) 213, 222
SexAI ACCWGGT 1 cut(s) 1006
SfaNI GCATC 2 cut(s) 217, 687
Sfr274I CTCGAG 1 cut(s) 952
SinI GGWCC 1 cut(s) 413
SlaI CTCGAG 1 cut(s) 952
SmiMI CAYNNNNRTG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 952
SmoI CTYRAG 1 cut(s) 952
Sse9I AATT 7 cut(s) 20, 171, 270, 345, 364, 688, 989
SsiI CCGC 1 cut(s) 380
SspMI CTAG 6 cut(s) 59, 72, 489, 495, 824, 873
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 399, 732, 1006
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 2 cut(s) 651, 753
TaiI ACGT 1 cut(s) 184
TaqI TCGA 4 cut(s) 204, 301, 465, 953
TasI AATT 7 cut(s) 20, 171, 270, 345, 364, 688, 989
TfiI GAWTC 2 cut(s) 68, 246
Tru1I TTAA 4 cut(s) 273, 807, 927, 1029
Tru9I TTAA 4 cut(s) 273, 807, 927, 1029
TscAI CASTG 3 cut(s) 505, 835, 1086
TseFI GTSAC 3 cut(s) 375, 865, 1002
Tsp45I GTSAC 3 cut(s) 375, 865, 1002
TspDTI ATGAA 1 cut(s) 1055
TspGWI ACGGA 2 cut(s) 527, 650
TspRI CASTG 3 cut(s) 505, 835, 1086
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 1003
XhoI CTCGAG 1 cut(s) 952
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 6 cut(s) 59, 72, 489, 495, 824, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.