Rh2DG516500

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000202
Physical Location & Seq
Reverse (-)
73994791 .. 74001739
6949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG516500.1

Sequence Viewer

Length: 2589 bp
ATGACACTGGAAGTTGCAGAGTTCTTGAGGCACAAGTTCTTGAAGTCCATTCAAGACGCAGAGTCTGAATTGTCAGGATTTGTGTTCCTCTCCCACTTCAAAGCAATCAAATGCTTCGTGGAAGAGATACAGATAATCCCGGAGATCAAAATCACTCTCGGCTCCGGCGACGACCTCTACCACAAGACCGTGTCTTTGCTCTACGAGCTCAACGACGCGTTGGCCGAATCCCGAGTATTGGCCAACGTGTGCAAGCTGCAAAATAAGAAGCTCTTTTTCTTGTTGCACCCGTTGTTGAACTACTCTATCCGAAAGATGAAGAAGCGACTCAACCAAATGAAACGCAAGTTTGCGGCTTTGGTCGAAGAGGAGAAAATGTATTCCACACTTGTAATTTGTGATCAGACGAGTCTAGACGGTGAGCCGGGAACTGAAATGGATGATGACCGTACGAGTCAAATCGGCGGCGCAGAAACGAGTATAGTCGGTGCTGATGAGCAGGCGGAGAAGATTGAGGGCTTGCTGTGTGGCGGTGCTACGGCAATTGGGATAGTCGGCATTGCCGGCGTAGGTAAGACCACTCTAGTGCACCAGGTTTTGAATAGGCAGAGAGTGAGGGATGAGTTTTCTCCTATAATTTGGTTATGCTTATCGGACAAAGAAAAATATGAATTTACTAATAGCATCGGCATTAGCATTGTGAGACGCATTCTTGATAAGTTAAGGGCCGTTGATAGTGGCAATGGTGCCATTGACAATCTCAATGATGCCATAGTGGAGGAGGACAGAGATATTTCTGGCCTCGGACTTGAGAGGCTCTTGGCAAGGCTTAACCGGCTTTTATCTGGTAAAAGATATTTGATTGTGTTGGATGATGTGTGGCACATTAGCGAATTTTATTCGGATTTAGGTTGTACAATTCAGGATCGGTTATCTTATGGATTGCCTAAGGGTAGTGGTGGTGCTGTTATTGTCACCACTAGGATACCAGAAGTTGCTGAACACATGGTTGGCAGAAATAAGTTGATTACTGTTGAGCCTTTGGACACAGAATGCTGCTGGCGAATATTTATGGAGACTATCAAGGATAATAAGGAAGTTTTAAACATTTCAACTCATGAAACTTTGGATAAGATTAAAAATGAAATTAAGGACCAATGTTACGGTCTACCATTGGCTGCTAAGGAGCTCGCAGGAATCATTCCCAAACGGATTCGTGAGATAGAGTCCAACCGCTTCTTGAAGGAGATGTACATTCCTGATGAATTGCTTCATGCTGATTTGGAAGTCGAACCTGCTGTCCATATACCAAAATTCCCAGTGTTGGTGTTTATTGATATGAAAAATATGAACAATAAACAGCTTGGAGAAAAACTCCTTGACAAATTTCGCTATCATCTTAATAAAAAGCAGGTTTTTGCTGTTTTAGAAGAGAAGTCTGATACGAAGAAAAAAGTAAAGGCACATGACCCTGAGAGGGTGCTAAAGGATGTTTATGGCACTTTAGAAAAGCTTAAGCGTAAAGGATATGGTTTTGCTGATGAAATTCAAAAGACGATGACGGTTATAATTGTTGGTGGTGATTCTGTGGCTAACTGGATTTTGGGAGTTATCTGTGATCTAAAATTGCCGGAGTTACCCTCCATTGCTCCAATTCCGCTACAAACCATTTCTCTTATTGGTGGTAGCATCTCAAGTTCCTTTGGATGGAGGAGCATTTCTCCTGACGATCCAGTGAAATTGTCCCTGCTAGATGTACAATATGCAAAAAAAATGAAAACTGACAGCTGGCATATTCTCATTTGGATGAAAACCATTAAAAGTTCTACAGCTAATCAACAAATACCTCATTCTTTGCATCTATGTCATCCTGTTCACGAACCGGATAAGCAAAATGCGGATAATCTCAAATTGTCCGGAGGGTTTTGGAACTACTTCAGCTTGGAAGTTGATGCTCTCCGACAATATGGAAATCACTGCTTTCATCCTACTGAAAGGACCTTATCATCAGTTGCAAATATAAAGGTTATGAAACAACTTGGTCAGTGGGATAAACTTGATATTACTAGCAGCATCAGGTCAATCCTTTGTTTTAACTTGTCTAGACCACAAATTATGAAGAAAGATCGGAAAGAGAGATACCTGTTTCCGTCTTTCATAGATGATGGACATCTTCAAATTATTGGTTACAGAGATGTTTTCTTTCCTCTTAGTGAAGGTTCCATTCACCTTGGCCAGGTACGGGGAATTCGTTTCGAGTTTATTGATGGTGAAGAAGAGGTTGTCAACATGACAATTGATGGAACACCATGGGAGAATATCCCACTTGATGGTACTGGTATCACTGTAATTGAAATCACTCATCACTGTCAAGTCAACATTCTCGCCTATCCATATTGCGATTGCCCACCAAAAAGTATCCACGATCAGGTGGTGATGAATGATGCTTCTGAAAATGATGATGAGAACAATGCTAAAGATTACTCTGAACGACATAAGAAGTTTGGTGCAGCAAGCACTTTCAAACTTAGCTGTGCTGATGAAGGACATGAAACCAATGGTGAAGCAAGCACTTCCAACGTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

862

Amino Acids

97.33

Weight (kDa)

5.91

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 162 - 297 3.2e-07 AAA ATPase domain
NB-ARC PF00931 167 - 364 4.7e-24 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1568
Acc36I ACCTGC 2 cut(s) 1303, 1402
AccB1I GGYRCC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 2330
AccI GTMKAC 1 cut(s) 1168
AccII CGCG 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 1916
AciI CCGC 7 cut(s) 353, 465, 503, 531, 1234, 1658, 1898
AclWI GGATC 2 cut(s) 933, 1724
AcoI YGGCCR 3 cut(s) 222, 240, 2233
AcsI RAATTY 6 cut(s) 671, 893, 1313, 1385, 1545, 2247
AcuI CTGAAG 1 cut(s) 1921
AfaI GTAC 6 cut(s) 451, 916, 1253, 1758, 2241, 2335
AfiI CCNNNNNNNGG 8 cut(s) 238, 1324, 1477, 1707, 2236, 2242, 2330, 2428
AflII CTTAAG 1 cut(s) 1514
AflIII ACRYGT 2 cut(s) 216, 246
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 2 cut(s) 591, 2235
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
AleI CACNNNNGTG 1 cut(s) 584
AloI GAACNNNNNNTCC 2 cut(s) 1284, 1316
Alw21I GWGCWC 3 cut(s) 210, 591, 1191
Alw26I GTCTC 2 cut(s) 697, 1070
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 2 cut(s) 933, 1724
AlwNI CAGNNNCTG 1 cut(s) 65
Ama87I CYCGRG 1 cut(s) 231
Aor13HI TCCGGA 1 cut(s) 1916
AoxI GGCC 5 cut(s) 222, 240, 726, 799, 2233
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 5 cut(s) 256, 1056, 1178, 2070, 2510
ApoI RAATTY 6 cut(s) 671, 893, 1313, 1385, 1545, 2247
ArsI GACNNNNNNTTYG 4 cut(s) 178, 210, 1284, 1316
Asp700I GAANNNNTTC 2 cut(s) 1269, 1934
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 3 cut(s) 726, 1153, 1998
AsuC2I CCSGG 2 cut(s) 140, 426
AsuHPI GGTGA 7 cut(s) 431, 967, 1592, 2219, 2282, 2446, 2573
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 2 cut(s) 1153, 1998
AxyI CCTNAGG 1 cut(s) 948
BaeGI GKGCMC 1 cut(s) 591
BalI TGGCCA 2 cut(s) 242, 2235
BanI GGYRCC 1 cut(s) 746
BanII GRGCYC 2 cut(s) 210, 1191
BarI GAAGNNNNNNTAC 2 cut(s) 1235, 1267
Bbv12I GWGCWC 3 cut(s) 210, 591, 1191
BbvI GCAGC 5 cut(s) 243, 1043, 1165, 2082, 2522
BccI CCATC 5 cut(s) 1701, 2159, 2261, 2294, 2324
BceAI ACGGC 2 cut(s) 555, 713
BciT130I CCWGG 2 cut(s) 593, 2237
BciVI GTATCC 2 cut(s) 978, 2429
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 2 cut(s) 140, 426
BcoDI GTCTC 2 cut(s) 697, 1070
BfaI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
BfmI CTRYAG 1 cut(s) 1827
BfrI CTTAAG 1 cut(s) 1514
BfuAI ACCTGC 2 cut(s) 1303, 1402
BfuI GTATCC 2 cut(s) 978, 2429
BisI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 2071, 2511
BlsI GCNGC 7 cut(s) 258, 355, 467, 1058, 1180, 2072, 2512
Bme1390I CCNGG 4 cut(s) 140, 426, 593, 2237
Bme18I GGWCC 2 cut(s) 1153, 1998
BmeRI GACNNNNNGTC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 3 cut(s) 726, 1153, 1998
BmiI GGNNCC 3 cut(s) 163, 748, 2221
BmrFI CCNGG 4 cut(s) 140, 426, 593, 2237
BmrI ACTGGG 1 cut(s) 1313
BmsI GCATC 7 cut(s) 693, 757, 1698, 1867, 1942, 2082, 2434
BmuI ACTGGG 1 cut(s) 1313
BplI GAGNNNNNCTC 6 cut(s) 1359, 1391, 1625, 1657, 1705, 1737
Bpu10I CCTNAGC 1 cut(s) 1182
BpuEI CTTGAG 3 cut(s) 46, 830, 1678
BpuMI CCSGG 2 cut(s) 140, 426
BsaBI GATNNNNATC 2 cut(s) 149, 2169
BsaJI CCNNGG 3 cut(s) 802, 2230, 2309
BsaWI WCCGGW 2 cut(s) 1882, 1916
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 8 cut(s) 238, 1324, 1477, 1707, 2236, 2242, 2330, 2428
Bse118I RCCGGY 2 cut(s) 563, 834
Bse1I ACTGG 5 cut(s) 12, 1319, 1601, 1733, 2341
Bse21I CCTNAGG 1 cut(s) 948
Bse3DI GCAATG 3 cut(s) 558, 748, 1644
Bse8I GATNNNNATC 2 cut(s) 149, 2169
BseAI TCCGGA 1 cut(s) 1916
BseBI CCWGG 2 cut(s) 593, 2237
BseDI CCNNGG 3 cut(s) 802, 2230, 2309
BseGI GGATG 8 cut(s) 445, 625, 877, 1495, 1712, 1812, 1867, 1984
BseJI GATNNNNATC 2 cut(s) 149, 2169
BseLI CCNNNNNNNGG 8 cut(s) 238, 1324, 1477, 1707, 2236, 2242, 2330, 2428
BseMI GCAATG 3 cut(s) 558, 748, 1644
BseMII CTCAG 1 cut(s) 1464
BseNI ACTGG 5 cut(s) 12, 1319, 1601, 1733, 2341
BseRI GAGGAG 3 cut(s) 383, 794, 1726
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 5 cut(s) 243, 1043, 1165, 2082, 2522
BsgI GTGCAG 1 cut(s) 2529
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BshNI GGYRCC 1 cut(s) 746
BsiHKAI GWGCWC 3 cut(s) 210, 591, 1191
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 8 cut(s) 140, 165, 425, 564, 835, 1631, 1883, 1917
BsiWI CGTACG 1 cut(s) 449
BslFI GGGAC 1 cut(s) 1729
BslI CCNNNNNNNGG 8 cut(s) 238, 1324, 1477, 1707, 2236, 2242, 2330, 2428
BsmAI GTCTC 2 cut(s) 697, 1070
BsmBI CGTCTC 1 cut(s) 697
BsmFI GGGAC 1 cut(s) 1729
BsmI GAATGC 2 cut(s) 708, 1058
BsnI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BsoBI CYCGRG 1 cut(s) 231
Bsp1286I GDGCHC 3 cut(s) 210, 591, 1191
Bsp13I TCCGGA 1 cut(s) 1916
Bsp1407I TGTACA 3 cut(s) 914, 1251, 1756
Bsp143I GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
Bsp19I CCATGG 1 cut(s) 2309
BspACI CCGC 7 cut(s) 353, 465, 503, 531, 1234, 1658, 1898
BspANI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BspCNI CTCAG 1 cut(s) 1465
BspEI TCCGGA 1 cut(s) 1916
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 3 cut(s) 163, 748, 2221
BspMI ACCTGC 2 cut(s) 1303, 1402
BspPI GGATC 2 cut(s) 933, 1724
BspT107I GGYRCC 1 cut(s) 746
BspTI CTTAAG 1 cut(s) 1514
BsrDI GCAATG 3 cut(s) 558, 748, 1644
BsrFI RCCGGY 2 cut(s) 563, 834
BsrGI TGTACA 3 cut(s) 914, 1251, 1756
BsrI ACTGG 5 cut(s) 12, 1319, 1601, 1733, 2341
BssAI RCCGGY 2 cut(s) 563, 834
BssECI CCNNGG 3 cut(s) 802, 2230, 2309
BssMI GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
BssT1I CCWWGG 2 cut(s) 2230, 2309
Bst2UI CCWGG 2 cut(s) 593, 2237
Bst4CI ACNGT 8 cut(s) 190, 419, 449, 1033, 1166, 1564, 2347, 2369
Bst6I CTCTTC 4 cut(s) 117, 360, 1425, 2271
BstAFI CTTAAG 1 cut(s) 1514
BstAUI TGTACA 3 cut(s) 914, 1251, 1756
BstC8I GCNNGC 9 cut(s) 254, 501, 521, 565, 1061, 1191, 1790, 2515, 2569
BstDEI CTNAG 5 cut(s) 948, 1182, 1473, 2210, 2528
BstDSI CCRYGG 1 cut(s) 2309
BstENI CCTNNNNNAGG 1 cut(s) 2234
BstF5I GGATG 8 cut(s) 445, 625, 877, 1495, 1712, 1812, 1867, 1984
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 7 cut(s) 147, 403, 928, 1621, 1732, 2128, 2428
BstMAI GTCTC 2 cut(s) 697, 1070
BstMBI GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
BstMWI GCNNNNNNNGC 3 cut(s) 205, 564, 835
BstNI CCWGG 2 cut(s) 593, 2237
BstSCI CCNGG 4 cut(s) 138, 424, 591, 2235
BstSFI CTRYAG 1 cut(s) 1827
BstSLI GKGCMC 1 cut(s) 591
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 5 cut(s) 243, 1043, 1165, 2082, 2522
Bsu36I CCTNAGG 1 cut(s) 948
BsuI GTATCC 2 cut(s) 978, 2429
BsuRI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BtgI CCRYGG 1 cut(s) 2309
BtsCI GGATG 8 cut(s) 445, 625, 877, 1495, 1712, 1812, 1867, 1984
BtsI GCAGTG 1 cut(s) 1975
BtsIMutI CAGTG 7 cut(s) 5, 1326, 1740, 1975, 2051, 2343, 2365
BveI ACCTGC 2 cut(s) 1303, 1402
Cac8I GCNNGC 9 cut(s) 254, 501, 521, 565, 1061, 1191, 1790, 2515, 2569
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 1 cut(s) 1117
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 2 cut(s) 563, 834
Cfr13I GGNCC 3 cut(s) 726, 1153, 1998
CseI GACGC 3 cut(s) 65, 224, 714
CsiI ACCWGGT 1 cut(s) 591
Csp6I GTAC 6 cut(s) 450, 915, 1252, 1757, 2240, 2334
CviAII CATG 7 cut(s) 1006, 1118, 1274, 1466, 2290, 2310, 2549
CviQI GTAC 6 cut(s) 450, 915, 1252, 1757, 2240, 2334
DdeI CTNAG 5 cut(s) 948, 1182, 1473, 2210, 2528
DpnI GATC 7 cut(s) 146, 402, 927, 1620, 1731, 2127, 2427
DpnII GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
DraI TTTAAA 1 cut(s) 1104
DriI GACNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 3 cut(s) 222, 240, 2233
Eam1104I CTCTTC 4 cut(s) 117, 360, 1425, 2271
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 4 cut(s) 117, 360, 1425, 2271
EciI GGCGGA 1 cut(s) 518
Ecl136II GAGCTC 2 cut(s) 208, 1189
Eco130I CCWWGG 2 cut(s) 2230, 2309
Eco24I GRGCYC 2 cut(s) 210, 1191
Eco47I GGWCC 2 cut(s) 1153, 1998
Eco53kI GAGCTC 2 cut(s) 208, 1189
Eco57I CTGAAG 1 cut(s) 1921
Eco81I CCTNAGG 1 cut(s) 948
Eco88I CYCGRG 1 cut(s) 231
EcoICRI GAGCTC 2 cut(s) 208, 1189
EcoNI CCTNNNNNAGG 1 cut(s) 2234
EcoO109I RGGNCCY 1 cut(s) 1998
EcoRI GAATTC 1 cut(s) 2247
EcoRII CCWGG 2 cut(s) 591, 2235
EcoT14I CCWWGG 2 cut(s) 2230, 2309
EcoT38I GRGCYC 2 cut(s) 210, 1191
ErhI CCWWGG 2 cut(s) 2230, 2309
Esp3I CGTCTC 1 cut(s) 697
FaeI CATG 7 cut(s) 1009, 1121, 1277, 1469, 2293, 2313, 2552
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 1729
FatI CATG 7 cut(s) 1005, 1117, 1273, 1465, 2289, 2309, 2548
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 2071, 2511
FokI GGATG 8 cut(s) 452, 632, 884, 1502, 1719, 1819, 1854, 1971
FriOI GRGCYC 2 cut(s) 210, 1191
Fsp4HI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 2071, 2511
FspBI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
GlaI GCGC 1 cut(s) 469
GluI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 2071, 2511
HaeIII GGCC 5 cut(s) 224, 242, 728, 801, 2235
HapII CCGG 8 cut(s) 140, 165, 425, 564, 835, 1631, 1883, 1917
HgaI GACGC 3 cut(s) 65, 224, 714
HhaI GCGC 1 cut(s) 470
Hin1II CATG 7 cut(s) 1009, 1121, 1277, 1469, 2293, 2313, 2552
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 2 cut(s) 2287, 2377
HindII GTYRAC 2 cut(s) 2287, 2377
HindIII AAGCTT 1 cut(s) 1511
HinfI GANTC 9 cut(s) 62, 227, 327, 409, 454, 1197, 1213, 1226, 1583
HpaII CCGG 8 cut(s) 140, 165, 425, 564, 835, 1631, 1883, 1917
HphI GGTGA 7 cut(s) 431, 967, 1592, 2219, 2282, 2446, 2573
Hpy166II GTNNAC 5 cut(s) 589, 1169, 1876, 2287, 2377
Hpy8I GTNNAC 5 cut(s) 589, 1169, 1876, 2287, 2377
Hpy99I CGWCG 2 cut(s) 173, 218
HpyAV CCTTC 3 cut(s) 1237, 2210, 2537
HpyCH4III ACNGT 8 cut(s) 190, 419, 449, 1033, 1166, 1564, 2347, 2369
HpyCH4IV ACGT 2 cut(s) 246, 2580
HpyCH4V TGCA 9 cut(s) 17, 252, 259, 286, 589, 1766, 1858, 2015, 2510
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 564, 835
HpyF3I CTNAG 5 cut(s) 948, 1182, 1473, 2210, 2528
HpySE526I ACGT 2 cut(s) 246, 2580
Hsp92II CATG 7 cut(s) 1009, 1121, 1277, 1469, 2293, 2313, 2552
HspAI GCGC 1 cut(s) 468
Kpn2I TCCGGA 1 cut(s) 1916
KroI GCCGGC 1 cut(s) 563
KroNI GCCGGC 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
LmnI GCTCC 4 cut(s) 167, 1186, 1654, 1713
Lsp1109I GCAGC 5 cut(s) 243, 1043, 1165, 2082, 2522
LweI GCATC 7 cut(s) 693, 757, 1698, 1867, 1942, 2082, 2434
MabI ACCWGGT 1 cut(s) 591
MaeI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
MaeII ACGT 2 cut(s) 246, 2580
MaeIII GTNAC 4 cut(s) 973, 1160, 1635, 2186
MalI GATC 7 cut(s) 146, 402, 927, 1620, 1731, 2127, 2427
MboI GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
MfeI CAATTG 2 cut(s) 543, 2295
MhlI GDGCHC 3 cut(s) 210, 591, 1191
MlsI TGGCCA 2 cut(s) 242, 2235
MluI ACGCGT 1 cut(s) 216
MluNI TGGCCA 2 cut(s) 242, 2235
MlyI GAGTC 5 cut(s) 71, 321, 418, 463, 1235
MmeI TCCRAC 3 cut(s) 849, 1254, 1984
Mox20I TGGCCA 2 cut(s) 242, 2235
MroI TCCGGA 1 cut(s) 1916
MroNI GCCGGC 1 cut(s) 563
MroXI GAANNNNTTC 2 cut(s) 1269, 1934
MscI TGGCCA 2 cut(s) 242, 2235
MseI TTAA 9 cut(s) 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2094
MslI CAYNNNNRTG 2 cut(s) 584, 1805
Msp20I TGGCCA 2 cut(s) 242, 2235
MspA1I CMGCKG 1 cut(s) 1788
MspCI CTTAAG 1 cut(s) 1514
MspI CCGG 8 cut(s) 140, 165, 425, 564, 835, 1631, 1883, 1917
MspR9I CCNGG 4 cut(s) 140, 426, 593, 2237
MunI CAATTG 2 cut(s) 543, 2295
Mva1269I GAATGC 2 cut(s) 708, 1058
MvaI CCWGG 2 cut(s) 593, 2237
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 3 cut(s) 205, 564, 835
NaeI GCCGGC 1 cut(s) 565
NciI CCSGG 2 cut(s) 140, 426
NcoI CCATGG 1 cut(s) 2309
NdeII GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
NgoMIV GCCGGC 1 cut(s) 563
NlaIII CATG 7 cut(s) 1009, 1121, 1277, 1469, 2293, 2313, 2552
NlaIV GGNNCC 3 cut(s) 163, 748, 2221
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 973
OliI CACNNNNGTG 1 cut(s) 584
PagI TCATGA 1 cut(s) 1117
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 210, 222, 2248
PctI GAATGC 2 cut(s) 708, 1058
PdiI GCCGGC 1 cut(s) 565
PdmI GAANNNNTTC 2 cut(s) 1269, 1934
PfeI GAWTC 4 cut(s) 227, 1197, 1213, 1583
Pfl23II CGTACG 1 cut(s) 449
PflFI GACNNNGTC 1 cut(s) 190
PflMI CCANNNNNTGG 1 cut(s) 2330
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 7 cut(s) 258, 355, 467, 1058, 1180, 2072, 2512
PleI GAGTC 5 cut(s) 70, 321, 417, 462, 1234
PpsI GAGTC 5 cut(s) 70, 321, 417, 462, 1234
PpuMI RGGWCCY 1 cut(s) 1998
PsiI TTATAA 1 cut(s) 1568
Psp124BI GAGCTC 2 cut(s) 210, 1191
Psp5II RGGWCCY 1 cut(s) 1998
Psp6I CCWGG 2 cut(s) 591, 2235
PspGI CCWGG 2 cut(s) 591, 2235
PspLI CGTACG 1 cut(s) 449
PspN4I GGNNCC 3 cut(s) 163, 748, 2221
PspPI GGNCC 3 cut(s) 726, 1153, 1998
PspPPI RGGWCCY 1 cut(s) 1998
PstNI CAGNNNCTG 1 cut(s) 65
PsyI GACNNNGTC 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 1788
RsaI GTAC 6 cut(s) 451, 916, 1253, 1758, 2241, 2335
RsaNI GTAC 6 cut(s) 450, 915, 1252, 1757, 2240, 2334
RseI CAYNNNNRTG 2 cut(s) 584, 1805
SacI GAGCTC 2 cut(s) 210, 1191
SaqAI TTAA 9 cut(s) 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2094
SatI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 2071, 2511
Sau3AI GATC 7 cut(s) 144, 400, 925, 1618, 1729, 2125, 2425
Sau96I GGNCC 3 cut(s) 726, 1153, 1998
SchI GAGTC 5 cut(s) 71, 321, 418, 463, 1235
ScrFI CCNGG 4 cut(s) 140, 426, 593, 2237
SduI GDGCHC 3 cut(s) 210, 591, 1191
SexAI ACCWGGT 1 cut(s) 591
SfaNI GCATC 7 cut(s) 693, 757, 1698, 1867, 1942, 2082, 2434
SfcI CTRYAG 1 cut(s) 1827
SinI GGWCC 2 cut(s) 1153, 1998
SmiMI CAYNNNNRTG 2 cut(s) 584, 1805
SmlI CTYRAG 4 cut(s) 25, 809, 1514, 1693
SmoI CTYRAG 4 cut(s) 25, 809, 1514, 1693
SsiI CCGC 7 cut(s) 353, 465, 503, 531, 1234, 1658, 1898
SspI AATATT 1 cut(s) 1068
SspMI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
SstI GAGCTC 2 cut(s) 210, 1191
StyD4I CCNGG 4 cut(s) 138, 424, 591, 2235
StyI CCWWGG 2 cut(s) 2230, 2309
TaaI ACNGT 8 cut(s) 190, 419, 449, 1033, 1166, 1564, 2347, 2369
TaiI ACGT 2 cut(s) 249, 2583
TaqI TCGA 3 cut(s) 363, 1290, 2256
TatI WGTACW 3 cut(s) 914, 1251, 1756
TauI GCSGC 2 cut(s) 356, 468
TfiI GAWTC 4 cut(s) 227, 1197, 1213, 1583
Tru1I TTAA 9 cut(s) 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2094
Tru9I TTAA 9 cut(s) 722, 831, 1103, 1137, 1149, 1401, 1515, 1818, 2094
TscAI CASTG 7 cut(s) 12, 1326, 1740, 1982, 2051, 2350, 2372
TseFI GTSAC 1 cut(s) 973
TseI GCWGC 5 cut(s) 256, 1056, 1178, 2070, 2510
Tsp45I GTSAC 1 cut(s) 973
TspGWI ACGGA 2 cut(s) 1225, 2139
TspRI CASTG 7 cut(s) 12, 1326, 1740, 1982, 2051, 2350, 2372
Tth111I GACNNNGTC 1 cut(s) 190
Van91I CCANNNNNTGG 1 cut(s) 2330
Vha464I CTTAAG 1 cut(s) 1514
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 2 cut(s) 1153, 1998
XagI CCTNNNNNAGG 1 cut(s) 2234
XapI RAATTY 6 cut(s) 671, 893, 1313, 1385, 1545, 2247
XbaI TCTAGA 2 cut(s) 412, 2102
XmiI GTMKAC 1 cut(s) 1168
XmnI GAANNNNTTC 2 cut(s) 1269, 1934
XspI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.