Rmu_sc0005294.1_g000007

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005294.1
OG0000202
Physical Location & Seq
Reverse (-)
20644 .. 34685
14042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005294.1_g000007.1.cds

Sequence Viewer

Length: 5082 bp
atgagaaaagaggagaggtgggttggggcttcgttagaagaaagggaggaatggctgtggacttgtggtctcggtgttggtcgaagggaagcagaagcgatggcggcagctgatgttgctcaatttctgtggaaaaagttcttgcggtctctagtggacgcagagtctgaattatcagaaatgttgatgccgtggaatgcatcatgggaagtgaatacttatgttcgggcaaatacttatctcttggagctcaactatgcactggcagagtgccaaatctttgcaaaggagtgcagggagcttaaaaagaagattttaccgttgaatccttccgctgtttttttcatcctacagatgaagaagaggctgacggcactgagaaggaagtttgagactttggtgcttgagagcccatcaatacctacttctatcttgagggagcgaaaagggagaaactctgtggaatgtttgggatttactgagaaggatatagtggtgttaaagaagaattcagaagaaactgtggtgttttctgttgcaatgaattcagaagactttacaactatggttgagctcttgggcttgcttggtggctctgcagctggaattggatttacatccattgggattgtcggcatggctcgtgtgggtaagtccattttagtacaaagacttgtggaagagacagctgtgctctctgagttttctcctatcattcagttatgcttgtctgatataatacaagaacataatgttgtagacaacactaatagtgccagtggggccagcattagcatcagtattgtgaaacttatacttgtaaagttgggggaggaggagactgccggtatttctggccttgaacttaattcgctcttggaaaggctcaaccaccttttatcaggtaaaaagtatttaattgtgttggatgatgtgtggcataatatggagttctgtagggaccttggctatcagcttcctgatggtgagaaatttggggatcggttatctgatgggttgcctaaggatggtggtggtgcagtcattgtggttagtaggataccagaagtggctctagatatggtggggatatgccaaagtactctgcataagcggctgaccatgctcaaaaggaagtctgcagtctccagtctggtgcatgaacggcaaaaacaccaaacacctatatctaacacacccagcccatcaatctctacaccaaaagagaagaccagtgtggcagaagaatgtttgccatttgctgagaaggtaaagaacatttcaagtgaaaacgtgccacttcttaatgtagggatatatagtgaatttaaggactcccgcatgcttgttggtgatccaactggaactggatttacagcaattgggatagttggcatggctggcgtcggtaagtccacttttgtgcacaatgtcttgaaaaccgtgtggtctgaatatgatcccatagtttggttatgcttgtcggatataatcaaaggtagaaaacagggaccaaatgtcactgccagtcatgaggtgatcagcattagcattgtggcatgtatcctcgaaatgttggagatttccatagcgaggcaagaccttggcattgccgagctcttggaaaggctaaatcatagtttatcgggtaaacggtatttgattgtgttggatgatgtgtgggacgttatggagttctgttcagatttatcttataagttacccgaaggtgagaaaattggggatcagttatctcacggattgcccaaggactgtggtggtacggtcattgtcactagtaggataactgaagtggctcaacatatggttggcttgggccaaaataacttgtttcttgttacgccttgggatagaggacagtgcttgttatacttcgacagctttatcttccatgaaagattacggtgtggtaaagaaaaacgttttggcgtgtcaaatacagaaactgtgagaaaggtccataatgaaatcctggatcaagtttgtggtctgccattaatgatcaaaactctcgcatacttaatctttgaaaaccagatcagtcagaatgattatagtaggtctgccctgaagaagatgttgatacctcgtgaatttcttattgattgttcatgggagtatgttccgatcaacaaaagaaatttcgactgggctcatgataaaatggacatgccaaaatgcccagtttttgtgtttattgatttccaaagcgaccttataggagctctagctgaagactttcgctaccagcttaatcgtgtgcaggttctttctgtgttacatgagagtttcattacgcgaagatgttcgccaatgaaaccagaaagggcgttagaggagttttatggttctctagcaaagcttaagggtgaaggacatgattttgctgatgaaattcgaaagaagatgacatttatagaatttgatgcaatgaaaccagtgaacaagatattggagacagttctaaagcatacaaaacatatcaaaacagatagctggcatatcctcatcaagatggaagccgccattcctacagattatcaagaaataccatattgtttgcagttatgtcatccagttcacaaaccaaatatggaaaatgtggataaccagccaataatccgtggagaattttggaactacttcagctttggtgttgacgatccaagattgtatggagctttgcgctttcgccacatttcaaggaccttaaacaggttacttgcaaaagtagaggttttgaaacatcacggtgatcagtggaaaacgcttagcatacctagtagcatcaggtcaattgtttgtcttaacttgcctagctttgttggcggactaaatccttggtcaaaagcaaatgtgaagaaagatcaaagtatagacttgaccccatctttgatagatgatggccgtcttgaaattattggttccagagatgatttgctttgtccgaagaaacggtggattcgccttgatcaggaaggacagggaatggcggcggccgatgttgctcagtttctgtggaaaaagtttttgaggtctcttgtagattcggagtctgaattatcaggtgctatgaatctcttctcttattttgaagcaatcaaaagtgtcttggtaaacacacagataattccattttctttagaaatgttgatgccatggaatgcgtcatgggaaatgagtacttatgttcgggctaatgcttatccgtctgagctcaattacgcattggcagagtgtcgaatctttgcaaatgagggcagggagatcaacaagaaggttttagtgtacagtccttccacggtttatttcctcctaaagatgaagaggcggttgaccacactgaaaaggaagtttgtgattttggtctctaatgcaagggagcagaaagagaggaacaacactgtcaaacgtttgggactacttactcaagagaaagtggctgtaaaggagaatttagaagaaatagtagtacttcctaatgcaagtacactggagattcatgaattgcttgttgatacagatcgagttggatttactccaattggaatagtaggcatggctggcgtgggtaaatctactatggtgcaagaggttttgacttttcatagagtctcaactgagttttcgcccatagtttggttatgcttgtcggatataatccaagaaaaagaactagtagatagtgctagtgccacttgggtcagcatcagcattgtgacatgcatacttgacaagttgggcgagaaagaaacaagtatttctggactcggccttgattcactcttggaaaggctcaaccatattttatcggctaaaaggtatctgattgtgttggatgatgtgtggcatactatggaattctactcggatataggtcataatcttccagaaggtgagaaatttggtgattgcctatcacacggattacctaagcattgtggtggtgcggtcattgtcactagtaggataagagaggtggctcaagatatggtaggccagaataatgtatttcttgctaggccttgggacaaaggatggtgctggatgcacttcaggtcctttgtcacaacgaaagcagaaggtttcggtgaagaaaaacgttttagcatgtcaaattgggaaatagtgcagaagattgagaatgaaattctggatcaaataggtggtctaccatcaattgctaaggcactcgcagaactaatagttgaaaaacggattagtgagactgatgataaggggctgaaagtatattttgttttcaaggttgttgctgttctagaccagagtttcattaataggcaaaagataaagacagaggaaccagagaaggcgctaaaagagttttatggcattctcgaaaatcttaagtgcaaaggacataaatttgctgatgagattcaaaagaagatgacaattatgattgttggcggggatgtttttgctaactggtttatgggagttatctgtgatctaaaattgccaggctcaccctccattgttcctatagcattgggaatcaaagctaacattgcaagttcatttggatgggagtttgatgctgataaagtaccggtgaacaagattttggggatgagaggcctgtcctcatctttcatagatgatggatatcttgaaattattggttcaaaagatgatttactttcccagaagaaaaggtggattcgccttgatcaggtaagagaaattcgcttcaagtttattgaggattcagttgaggttgtaaacatgatgatcgatggagtactgtggaaaaaaaccccacttgatggtttagtcgagattgaaatctcttacaactgtcaagttaacattcttgttaaacaagattactcagcaagaagtatccatgactcatcgcaaccctgtgggtcagaaactgctgatgatgacaatgctgaaggacgtaagaagagtggtggagcaagcacatcaagtttcctcattgatgataatgagcttgaagagaaatctcaaggacataaaaaaattggtgcagcaagcagctttaaaatctttgaggaggatgacattaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

1693

Amino Acids

190.84

Weight (kDa)

5.99

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1716
Acc36I ACCTGC 1 cut(s) 2275
AccB7I CCANNNNNTGG 3 cut(s) 1470, 3660, 4811
AccI GTMKAC 2 cut(s) 759, 4192
AccII CGCG 1 cut(s) 2320
AclI AACGTT 3 cut(s) 1942, 3433, 4123
AclWI GGATC 7 cut(s) 1008, 1349, 1454, 1752, 2004, 2678, 4185
AcoI YGGCCR 2 cut(s) 2926, 3018
AcuI CTGAAG 6 cut(s) 1830, 2111, 2274, 2650, 4060, 4962
AcyI GRCGYC 1 cut(s) 1404
AfaI GTAC 9 cut(s) 666, 1102, 1783, 3205, 3311, 3495, 3511, 4593, 4788
AfiI CCNNNNNNNGG 8 cut(s) 1028, 1470, 1593, 2736, 2995, 3660, 4717, 4811
AflII CTTAAG 2 cut(s) 2384, 4388
AgeI ACCGGT 1 cut(s) 4594
AhdI GACNNNNNGTC 3 cut(s) 66, 163, 1517
AhlI ACTAGT 3 cut(s) 1796, 3697, 3983
AjnI CCWGG 2 cut(s) 1992, 4504
AjuI GAANNNNNNNTTGG 6 cut(s) 1929, 1961, 2456, 2488, 4592, 4624
AleI CACNNNNGTG 1 cut(s) 1421
Alw21I GWGCWC 7 cut(s) 252, 576, 696, 1428, 1620, 2248, 3240
Alw44I GTGCAC 1 cut(s) 1424
AlwI GGATC 7 cut(s) 1008, 1349, 1454, 1752, 2004, 2678, 4185
AlwNI CAGNNNCTG 4 cut(s) 167, 1967, 3037, 4922
AoxI GGCC 9 cut(s) 783, 856, 1837, 2926, 3018, 3793, 4018, 4043, 4621
ApaLI GTGCAC 1 cut(s) 1424
ApeKI GCWGC 4 cut(s) 107, 599, 5039, 5046
ArsI GACNNNNNNTTYG 4 cut(s) 2896, 2928, 4174, 4206
AseI ATTAAT 3 cut(s) 2018, 4317, 5076
AsiGI ACCGGT 1 cut(s) 4594
Asp700I GAANNNNTTC 5 cut(s) 137, 2259, 2326, 2663, 3101
AspLEI GCGC 2 cut(s) 2709, 4357
AspS9I GGNCC 7 cut(s) 783, 961, 1511, 1837, 1978, 2727, 4080
AsuII TTCGAA 1 cut(s) 2419
AvaII GGWCC 5 cut(s) 961, 1511, 1978, 2727, 4080
AxyI CCTNAGG 1 cut(s) 1023
BaeGI GKGCMC 1 cut(s) 1428
BanII GRGCYC 7 cut(s) 252, 413, 576, 1620, 2176, 2248, 3240
BauI CACGAG 2 cut(s) 642, 2109
BbsI GAAGAC 3 cut(s) 558, 1235, 2262
Bbv12I GWGCWC 7 cut(s) 252, 576, 696, 1428, 1620, 2248, 3240
BbvI GCAGC 4 cut(s) 119, 611, 5051, 5058
BceAI ACGGC 4 cut(s) 175, 387, 1181, 2913
BciT130I CCWGG 2 cut(s) 1994, 4506
BciVI GTATCC 3 cut(s) 1053, 1574, 4898
BclI TGATCA 5 cut(s) 1539, 2022, 2776, 2992, 4714
BcuI ACTAGT 3 cut(s) 1796, 3697, 3983
BfmI CTRYAG 6 cut(s) 350, 597, 955, 1140, 2553, 4527
BfoI RGCGCY 1 cut(s) 4358
BfrI CTTAAG 2 cut(s) 2384, 4388
BfuAI ACCTGC 1 cut(s) 2275
BfuI GTATCC 3 cut(s) 1053, 1574, 4898
BglI GCCNNNNNGGC 1 cut(s) 782
BisI GCNGC 9 cut(s) 105, 108, 600, 1115, 2544, 3015, 3018, 5040, 5047
BlpI GCTNAGC 1 cut(s) 2792
BlsI GCNGC 9 cut(s) 106, 109, 601, 1116, 2545, 3016, 3019, 5041, 5048
BmcAI AGTACT 4 cut(s) 1102, 3205, 3495, 4788
Bme1390I CCNGG 2 cut(s) 1994, 4506
Bme18I GGWCC 5 cut(s) 961, 1511, 1978, 2727, 4080
BmeRI GACNNNNNGTC 3 cut(s) 66, 163, 1517
BmgT120I GGNCC 7 cut(s) 783, 961, 1511, 1837, 1978, 2727, 4080
BmiI GGNNCC 5 cut(s) 784, 962, 1512, 2947, 4344
BmrFI CCNGG 2 cut(s) 1994, 4506
BmrI ACTGGG 2 cut(s) 2179, 2198
BmsI GCATC 9 cut(s) 177, 209, 804, 2437, 2817, 3165, 3738, 4059, 4570
BmuI ACTGGG 2 cut(s) 2179, 2198
BpiI GAAGAC 3 cut(s) 558, 1235, 2262
BpmI CTGGAG 2 cut(s) 1132, 3536
Bpu10I CCTNAGC 2 cut(s) 3954, 4206
Bpu1102I GCTNAGC 1 cut(s) 2792
Bpu14I TTCGAA 1 cut(s) 2419
BpuEI CTTGAG 5 cut(s) 425, 454, 3435, 3990, 5001
Bsa29I ATCGAT 1 cut(s) 4779
BsaBI GATNNNNATC 4 cut(s) 616, 3543, 4650, 4829
BsaHI GRCGYC 1 cut(s) 1404
BsaI GGTCTC 4 cut(s) 74, 153, 3063, 3394
BsaWI WCCGGW 1 cut(s) 4594
Bsc4I CCNNNNNNNGG 8 cut(s) 1028, 1470, 1593, 2736, 2995, 3660, 4717, 4811
Bse118I RCCGGY 2 cut(s) 845, 4594
Bse21I CCTNAGG 1 cut(s) 1023
Bse3DI GCAATG 4 cut(s) 546, 1608, 2457, 4551
Bse8I GATNNNNATC 4 cut(s) 616, 3543, 4650, 4829
BseBI CCWGG 2 cut(s) 1994, 4506
BseCI ATCGAT 1 cut(s) 4779
BseJI GATNNNNATC 4 cut(s) 616, 3543, 4650, 4829
BseLI CCNNNNNNNGG 8 cut(s) 1028, 1470, 1593, 2736, 2995, 3660, 4717, 4811
BseMI GCAATG 4 cut(s) 546, 1608, 2457, 4551
BseMII CTCAG 8 cut(s) 368, 471, 690, 1254, 3044, 3225, 3633, 4890
BseRI GAGGAG 5 cut(s) 26, 848, 851, 2372, 5078
BseSI GKGCMC 1 cut(s) 1428
BseX3I CGGCCG 1 cut(s) 3018
BseXI GCAGC 4 cut(s) 119, 611, 5051, 5058
BseYI CCCAGC 1 cut(s) 1199
BsgI GTGCAG 5 cut(s) 313, 1059, 2303, 4172, 5058
Bsh1236I CGCG 1 cut(s) 2320
Bsh1285I CGRYCG 1 cut(s) 3021
BshFI GGCC 9 cut(s) 785, 858, 1839, 2928, 3020, 3795, 4020, 4045, 4623
BshTI ACCGGT 1 cut(s) 4594
BshVI ATCGAT 1 cut(s) 4779
BsiEI CGRYCG 1 cut(s) 3021
BsiHKAI GWGCWC 7 cut(s) 252, 576, 696, 1428, 1620, 2248, 3240
BsiSI CCGG 2 cut(s) 846, 4595
BslFI GGGAC 5 cut(s) 974, 1524, 1697, 3453, 4064
BslI CCNNNNNNNGG 8 cut(s) 1028, 1470, 1593, 2736, 2995, 3660, 4717, 4811
BsmFI GGGAC 5 cut(s) 974, 1524, 1697, 3453, 4064
BsmI GAATGC 3 cut(s) 202, 3190, 4374
BsnI GGCC 9 cut(s) 785, 858, 1839, 2928, 3020, 3795, 4020, 4045, 4623
Bso31I GGTCTC 4 cut(s) 74, 153, 3063, 3394
Bsp119I TTCGAA 1 cut(s) 2419
Bsp1286I GDGCHC 9 cut(s) 252, 413, 576, 696, 1428, 1620, 2176, 2248, 3240
Bsp1407I TGTACA 1 cut(s) 3309
Bsp1720I GCTNAGC 1 cut(s) 2792
Bsp19I CCATGG 1 cut(s) 3179
BspANI GGCC 9 cut(s) 785, 858, 1839, 2928, 3020, 3795, 4020, 4045, 4623
BspCNI CTCAG 8 cut(s) 369, 472, 691, 1255, 3043, 3226, 3634, 4889
BspDI ATCGAT 1 cut(s) 4779
BspFNI CGCG 1 cut(s) 2320
BspHI TCATGA 3 cut(s) 1531, 2176, 3523
BspLI GGNNCC 5 cut(s) 784, 962, 1512, 2947, 4344
BspMAI CTGCAG 2 cut(s) 601, 1144
BspMI ACCTGC 1 cut(s) 2275
BspPI GGATC 7 cut(s) 1008, 1349, 1454, 1752, 2004, 2678, 4185
BspT104I TTCGAA 1 cut(s) 2419
BspTI CTTAAG 2 cut(s) 2384, 4388
BspTNI GGTCTC 4 cut(s) 74, 153, 3063, 3394
BsrDI GCAATG 4 cut(s) 546, 1608, 2457, 4551
BsrFI RCCGGY 2 cut(s) 845, 4594
BsrGI TGTACA 1 cut(s) 3309
BssAI RCCGGY 2 cut(s) 845, 4594
BssNI GRCGYC 1 cut(s) 1404
BssSI CACGAG 2 cut(s) 642, 2109
BssT1I CCWWGG 7 cut(s) 964, 1603, 1767, 1865, 2861, 3179, 4046
Bst2BI CACGAG 2 cut(s) 642, 2109
Bst2UI CCWGG 2 cut(s) 1994, 4506
Bst6I CTCTTC 6 cut(s) 356, 675, 3107, 3341, 4949, 5001
BstACI GRCGYC 1 cut(s) 1404
BstAFI CTTAAG 2 cut(s) 2384, 4388
BstAUI TGTACA 1 cut(s) 3309
BstBI TTCGAA 1 cut(s) 2419
BstC8I GCNNGC 8 cut(s) 584, 787, 1343, 1402, 2519, 3586, 4969, 5044
BstDSI CCRYGG 4 cut(s) 191, 2644, 3179, 3321
BstENI CCTNNNNNAGG 3 cut(s) 2734, 2993, 4715
BstFNI CGCG 1 cut(s) 2320
BstH2I RGCGCY 1 cut(s) 4358
BstHHI GCGC 2 cut(s) 2709, 4357
BstMCI CGRYCG 1 cut(s) 3021
BstNI CCWGG 2 cut(s) 1994, 4506
BstNSI RCATGY 5 cut(s) 1345, 1563, 2194, 3747, 4135
BstSCI CCNGG 2 cut(s) 1992, 4504
BstSFI CTRYAG 6 cut(s) 350, 597, 955, 1140, 2553, 4527
BstSLI GKGCMC 1 cut(s) 1428
BstUI CGCG 1 cut(s) 2320
BstV1I GCAGC 4 cut(s) 119, 611, 5051, 5058
BstV2I GAAGAC 3 cut(s) 558, 1235, 2262
BstZI CGGCCG 1 cut(s) 3018
Bsu15I ATCGAT 1 cut(s) 4779
Bsu36I CCTNAGG 1 cut(s) 1023
BsuI GTATCC 3 cut(s) 1053, 1574, 4898
BsuRI GGCC 9 cut(s) 785, 858, 1839, 2928, 3020, 3795, 4020, 4045, 4623
BsuTUI ATCGAT 1 cut(s) 4779
BtgI CCRYGG 4 cut(s) 191, 2644, 3179, 3321
BtgZI GCGATG 2 cut(s) 113, 4884
BtsI GCAGTG 1 cut(s) 1521
BveI ACCTGC 1 cut(s) 2275
Cac8I GCNNGC 8 cut(s) 584, 787, 1343, 1402, 2519, 3586, 4969, 5044
CaiI CAGNNNCTG 4 cut(s) 167, 1967, 3037, 4922
CciI TCATGA 3 cut(s) 1531, 2176, 3523
CfoI GCGC 2 cut(s) 2709, 4357
Cfr10I RCCGGY 2 cut(s) 845, 4594
Cfr13I GGNCC 7 cut(s) 783, 961, 1511, 1837, 1978, 2727, 4080
ClaI ATCGAT 1 cut(s) 4779
CseI GACGC 3 cut(s) 167, 1393, 3177
Csp6I GTAC 9 cut(s) 665, 1101, 1782, 3204, 3310, 3494, 3510, 4592, 4787
CspAI ACCGGT 1 cut(s) 4594
CspCI CAANNNNNGTGG 4 cut(s) 110, 145, 1756, 1791
CviQI GTAC 9 cut(s) 665, 1101, 1782, 3204, 3310, 3494, 3510, 4592, 4787
DraI TTTAAA 1 cut(s) 5053
DriI GACNNNNNGTC 3 cut(s) 66, 163, 1517
EaeI YGGCCR 2 cut(s) 2926, 3018
EagI CGGCCG 1 cut(s) 3018
Eam1104I CTCTTC 6 cut(s) 356, 675, 3107, 3341, 4949, 5001
Eam1105I GACNNNNNGTC 3 cut(s) 66, 163, 1517
EarI CTCTTC 6 cut(s) 356, 675, 3107, 3341, 4949, 5001
EciI GGCGGA 1 cut(s) 2865
Ecl136II GAGCTC 5 cut(s) 250, 574, 1618, 2246, 3238
EclXI CGGCCG 1 cut(s) 3018
Eco130I CCWWGG 7 cut(s) 964, 1603, 1767, 1865, 2861, 3179, 4046
Eco147I AGGCCT 2 cut(s) 4045, 4623
Eco24I GRGCYC 7 cut(s) 252, 413, 576, 1620, 2176, 2248, 3240
Eco31I GGTCTC 4 cut(s) 74, 153, 3063, 3394
Eco32I GATATC 1 cut(s) 4652
Eco47I GGWCC 5 cut(s) 961, 1511, 1978, 2727, 4080
Eco52I CGGCCG 1 cut(s) 3018
Eco53kI GAGCTC 5 cut(s) 250, 574, 1618, 2246, 3238
Eco57I CTGAAG 6 cut(s) 1830, 2111, 2274, 2650, 4060, 4962
Eco81I CCTNAGG 1 cut(s) 1023
EcoICRI GAGCTC 5 cut(s) 250, 574, 1618, 2246, 3238
EcoNI CCTNNNNNAGG 3 cut(s) 2734, 2993, 4715
EcoO109I RGGNCCY 3 cut(s) 961, 2727, 4080
EcoRI GAATTC 3 cut(s) 508, 544, 3881
EcoRII CCWGG 2 cut(s) 1992, 4504
EcoRV GATATC 1 cut(s) 4652
EcoT14I CCWWGG 7 cut(s) 964, 1603, 1767, 1865, 2861, 3179, 4046
EcoT22I ATGCAT 2 cut(s) 202, 3749
EcoT38I GRGCYC 7 cut(s) 252, 413, 576, 1620, 2176, 2248, 3240
ErhI CCWWGG 7 cut(s) 964, 1603, 1767, 1865, 2861, 3179, 4046
FaqI GGGAC 5 cut(s) 974, 1524, 1697, 3453, 4064
FauI CCCGC 2 cut(s) 1346, 4445
FauNDI CATATG 1 cut(s) 1824
FbaI TGATCA 5 cut(s) 1539, 2022, 2776, 2992, 4714
FblI GTMKAC 2 cut(s) 759, 4192
Fnu4HI GCNGC 9 cut(s) 105, 108, 600, 1115, 2544, 3015, 3018, 5040, 5047
FriOI GRGCYC 7 cut(s) 252, 413, 576, 1620, 2176, 2248, 3240
Fsp4HI GCNGC 9 cut(s) 105, 108, 600, 1115, 2544, 3015, 3018, 5040, 5047
GlaI GCGC 2 cut(s) 2708, 4356
GluI GCNGC 9 cut(s) 105, 108, 600, 1115, 2544, 3015, 3018, 5040, 5047
GsaI CCCAGC 1 cut(s) 1203
GsuI CTGGAG 2 cut(s) 1132, 3536
HaeII RGCGCY 1 cut(s) 4358
HaeIII GGCC 9 cut(s) 785, 858, 1839, 2928, 3020, 3795, 4020, 4045, 4623
HapII CCGG 2 cut(s) 846, 4595
HgaI GACGC 3 cut(s) 167, 1393, 3177
HhaI GCGC 2 cut(s) 2709, 4357
Hin1I GRCGYC 1 cut(s) 1404
Hin6I GCGC 2 cut(s) 2707, 4355
HinP1I GCGC 2 cut(s) 2707, 4355
HincII GTYRAC 3 cut(s) 2680, 3357, 4852
HindII GTYRAC 3 cut(s) 2680, 3357, 4852
HindIII AAGCTT 1 cut(s) 2381
HpaI GTTAAC 1 cut(s) 4852
HpaII CCGG 2 cut(s) 846, 4595
Hpy99I CGWCG 1 cut(s) 1409
HpyCH4IV ACGT 6 cut(s) 1293, 1686, 1942, 3433, 4123, 4948
HpySE526I ACGT 6 cut(s) 1293, 1686, 1942, 3433, 4123, 4948
Hsp92I GRCGYC 1 cut(s) 1404
HspAI GCGC 2 cut(s) 2707, 4355
Ksp22I TGATCA 5 cut(s) 1539, 2022, 2776, 2992, 4714
KspAI GTTAAC 1 cut(s) 4852
LmnI GCTCC 7 cut(s) 247, 298, 439, 2243, 2699, 3403, 4964
Lsp1109I GCAGC 4 cut(s) 119, 611, 5051, 5058
LweI GCATC 9 cut(s) 177, 209, 804, 2437, 2817, 3165, 3738, 4059, 4570
MaeII ACGT 6 cut(s) 1293, 1686, 1942, 3433, 4123, 4948
MaeIII GTNAC 9 cut(s) 1519, 1719, 1792, 1858, 2298, 2739, 3739, 3979, 4087
MfeI CAATTG 4 cut(s) 1380, 2817, 3564, 4200
MhlI GDGCHC 9 cut(s) 252, 413, 576, 696, 1428, 1620, 2176, 2248, 3240
MlyI GAGTC 6 cut(s) 173, 1328, 3083, 3642, 3783, 4889
MmeI TCCRAC 8 cut(s) 906, 1382, 1464, 1557, 1650, 3532, 3654, 3837
Mph1103I ATGCAT 2 cut(s) 202, 3749
MroXI GAANNNNTTC 5 cut(s) 137, 2259, 2326, 2663, 3101
MslI CAYNNNNRTG 5 cut(s) 1421, 2534, 2772, 3963, 4567
MspA1I CMGCKG 4 cut(s) 110, 335, 602, 689
MspCI CTTAAG 2 cut(s) 2384, 4388
MspI CCGG 2 cut(s) 846, 4595
MspR9I CCNGG 2 cut(s) 1994, 4506
MunI CAATTG 4 cut(s) 1380, 2817, 3564, 4200
Mva1269I GAATGC 3 cut(s) 202, 3190, 4374
MvaI CCWGG 2 cut(s) 1994, 4506
MvnI CGCG 1 cut(s) 2320
NcoI CCATGG 1 cut(s) 3179
NdeI CATATG 1 cut(s) 1824
NlaIV GGNNCC 5 cut(s) 784, 962, 1512, 2947, 4344
NmeAIII GCCGAG 2 cut(s) 1639, 3771
NmuCI GTSAC 5 cut(s) 1519, 1792, 3739, 3979, 4087
NsiI ATGCAT 2 cut(s) 202, 3749
NspI RCATGY 5 cut(s) 1345, 1563, 2194, 3747, 4135
NspV TTCGAA 1 cut(s) 2419
OliI CACNNNNGTG 1 cut(s) 1421
PaeI GCATGC 1 cut(s) 1345
PagI TCATGA 3 cut(s) 1531, 2176, 3523
PceI AGGCCT 2 cut(s) 4045, 4623
PctI GAATGC 3 cut(s) 202, 3190, 4374
PdmI GAANNNNTTC 5 cut(s) 137, 2259, 2326, 2663, 3101
PflMI CCANNNNNTGG 3 cut(s) 1470, 3660, 4811
PfoI TCCNGGA 1 cut(s) 1992
PinAI ACCGGT 1 cut(s) 4594
PkrI GCNGC 9 cut(s) 106, 109, 601, 1116, 2545, 3016, 3019, 5041, 5048
PleI GAGTC 6 cut(s) 172, 1328, 3082, 3641, 3783, 4889
PpsI GAGTC 6 cut(s) 172, 1328, 3082, 3641, 3783, 4889
PpuMI RGGWCCY 3 cut(s) 961, 2727, 4080
PshBI ATTAAT 3 cut(s) 2018, 4317, 5076
PsiI TTATAA 1 cut(s) 1716
Psp124BI GAGCTC 5 cut(s) 252, 576, 1620, 2248, 3240
Psp1406I AACGTT 3 cut(s) 1942, 3433, 4123
Psp5II RGGWCCY 3 cut(s) 961, 2727, 4080
Psp6I CCWGG 2 cut(s) 1992, 4504
PspFI CCCAGC 1 cut(s) 1199
PspGI CCWGG 2 cut(s) 1992, 4504
PspN4I GGNNCC 5 cut(s) 784, 962, 1512, 2947, 4344
PspPI GGNCC 7 cut(s) 783, 961, 1511, 1837, 1978, 2727, 4080
PspPPI RGGWCCY 3 cut(s) 961, 2727, 4080
PsrI GAACNNNNNNTAC 2 cut(s) 1357, 1389
PstI CTGCAG 2 cut(s) 601, 1144
PstNI CAGNNNCTG 4 cut(s) 167, 1967, 3037, 4922
PvuII CAGCTG 3 cut(s) 110, 602, 689
RsaI GTAC 9 cut(s) 666, 1102, 1783, 3205, 3311, 3495, 3511, 4593, 4788
RsaNI GTAC 9 cut(s) 665, 1101, 1782, 3204, 3310, 3494, 3510, 4592, 4787
RseI CAYNNNNRTG 5 cut(s) 1421, 2534, 2772, 3963, 4567
SacI GAGCTC 5 cut(s) 252, 576, 1620, 2248, 3240
SatI GCNGC 9 cut(s) 105, 108, 600, 1115, 2544, 3015, 3018, 5040, 5047
Sau96I GGNCC 7 cut(s) 783, 961, 1511, 1837, 1978, 2727, 4080
ScaI AGTACT 4 cut(s) 1102, 3205, 3495, 4788
SchI GAGTC 6 cut(s) 173, 1328, 3083, 3642, 3783, 4889
ScrFI CCNGG 2 cut(s) 1994, 4506
SduI GDGCHC 9 cut(s) 252, 413, 576, 696, 1428, 1620, 2176, 2248, 3240
SfaNI GCATC 9 cut(s) 177, 209, 804, 2437, 2817, 3165, 3738, 4059, 4570
SfcI CTRYAG 6 cut(s) 350, 597, 955, 1140, 2553, 4527
SfuI TTCGAA 1 cut(s) 2419
SinI GGWCC 5 cut(s) 961, 1511, 1978, 2727, 4080
SmiMI CAYNNNNRTG 5 cut(s) 1421, 2534, 2772, 3963, 4567
SmlI CTYRAG 7 cut(s) 404, 433, 2384, 3450, 4005, 4388, 5016
SmoI CTYRAG 7 cut(s) 404, 433, 2384, 3450, 4005, 4388, 5016
SpeI ACTAGT 3 cut(s) 1796, 3697, 3983
SphI GCATGC 1 cut(s) 1345
SseBI AGGCCT 2 cut(s) 4045, 4623
SstI GAGCTC 5 cut(s) 252, 576, 1620, 2248, 3240
StuI AGGCCT 2 cut(s) 4045, 4623
StyD4I CCNGG 2 cut(s) 1992, 4504
StyI CCWWGG 7 cut(s) 964, 1603, 1767, 1865, 2861, 3179, 4046
TaiI ACGT 6 cut(s) 1296, 1689, 1945, 3436, 4126, 4951
TatI WGTACW 7 cut(s) 664, 1100, 3203, 3309, 3493, 3509, 4786
TauI GCSGC 5 cut(s) 107, 1117, 2546, 3017, 3020
TseFI GTSAC 5 cut(s) 1519, 1792, 3739, 3979, 4087
TseI GCWGC 4 cut(s) 107, 599, 5039, 5046
Tsp45I GTSAC 5 cut(s) 1519, 1792, 3739, 3979, 4087
TspGWI ACGGA 5 cut(s) 1773, 2633, 3219, 3960, 4252
Van91I CCANNNNNTGG 3 cut(s) 1470, 3660, 4811
Vha464I CTTAAG 2 cut(s) 2384, 4388
VneI GTGCAC 1 cut(s) 1424
VpaK11BI GGWCC 5 cut(s) 961, 1511, 1978, 2727, 4080
VspI ATTAAT 3 cut(s) 2018, 4317, 5076
XagI CCTNNNNNAGG 3 cut(s) 2734, 2993, 4715
XbaI TCTAGA 2 cut(s) 1075, 4300
XceI RCATGY 5 cut(s) 1345, 1563, 2194, 3747, 4135
XmiI GTMKAC 2 cut(s) 759, 4192
XmnI GAANNNNTTC 5 cut(s) 137, 2259, 2326, 2663, 3101
ZrmI AGTACT 4 cut(s) 1102, 3205, 3495, 4788
Zsp2I ATGCAT 2 cut(s) 202, 3749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.