Rh2AG493200

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000202
Physical Location & Seq
Forward (+)
71466209 .. 71470246
4038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG493200.1

Sequence Viewer

Length: 1008 bp
ATGTACTTCTTTGTTATTGAACTTGCAGATCAGCTTCAAAAATTGAAGGTGGAACCGGTATTGCTCCATTACTTTTCACACATTAAAGTTCTCCAGGGCATGGAGCTGAGAATGGCCACAAGTACAAGGTTAAAGACTTGTTTGTATAACTTCACTTCTCCTGGTGGTCCAATGTACCCTACAAGAGTTGTTCGTCATGCTGCCTGGGATGCTTTAGATTTACTTTTTCCATTATCTCTTGATTTTAGATTGGCAATAAGCTCAAGAAGTTTGCGACTTGTAAATATGTTACATATTTGTCAGGAGCATGAACTATCAAAATGTGATTTCAAGCTGTTGAGTAGCTTGAAAGTTTTGTGGATTACTTTCATTAAAAATATAAAGCGTCAGCTGACCGTAATAAAAAAGGACCTTGAGAAATTGCGGCAACAAGAAATTGGCAATCGTGAGGCAAAGAAAGGAGTCACCGGACCTAAACTTTCAGCAGAAGTTTTGGGATTTGATAAGCAATCAGAGGAGATTGAGGACTTACTACTTGAAGCAGGGGGTTCCAATTCCAGCAGTGCTGGTGAATTGAGGGCAATTGGGGTAGTTGGTATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTGGAGAGCAAGAGAGTGAAGCTGAAGTTTAATCATATACTTTGGTTACCCTTTTCGTTGAGTGAAGAAGAACAACAATATAGGAAGTCCAGCTTTGAAACATGTATCCATGATAATAATGGCCAAATCATAGAGGCGAGAATTGTTGGCCTTGACGAGCTCCTGGAAAGGCTAAGCCGCCAGTTATCGGATAAATGCTATTTGATTGTGTTGGATGATGTGTGTCATCGGCACATTAACATTATGGAACGGTTATGCAGCGGATTGCCCAGGCATAATGGTGGTGTTGTCATTGTCACTACTAGATTGAAGGTAGTGGCTCAAAAGCTGGAGAAACAACATAGATTGCAATTGACTTGGCCAGTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

335

Amino Acids

38.2

Weight (kDa)

9.22

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 190 - 325 8.2e-20 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 100
AciI CCGC 3 cut(s) 424, 814, 897
AcoI YGGCCR 3 cut(s) 114, 757, 995
AcuI CTGAAG 1 cut(s) 680
AfaI GTAC 4 cut(s) 5, 124, 176, 1002
AfiI CCNNNNNNNGG 2 cut(s) 100, 823
AflIII ACRYGT 1 cut(s) 737
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 8 cut(s) 20, 38, 46, 331, 349, 539, 734, 946
AjnI CCWGG 6 cut(s) 93, 160, 203, 621, 798, 905
Alw21I GWGCWC 1 cut(s) 798
AoxI GGCC 4 cut(s) 114, 757, 784, 995
ApeKI GCWGC 2 cut(s) 200, 894
AsiGI ACCGGT 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 167, 409, 470
AsuHPI GGTGA 2 cut(s) 457, 581
AvaII GGWCC 3 cut(s) 167, 409, 470
BalI TGGCCA 3 cut(s) 116, 759, 997
BanII GRGCYC 1 cut(s) 798
Bbv12I GWGCWC 1 cut(s) 798
BbvI GCAGC 2 cut(s) 187, 906
BciT130I CCWGG 6 cut(s) 95, 162, 205, 623, 800, 907
BciVI GTATCC 1 cut(s) 752
BfaI CTAG 1 cut(s) 939
BfuI GTATCC 1 cut(s) 752
BisI GCNGC 4 cut(s) 201, 425, 814, 895
BlpI GCTNAGC 1 cut(s) 809
BlsI GCNGC 4 cut(s) 202, 426, 815, 896
Bme1390I CCNGG 6 cut(s) 95, 162, 205, 623, 800, 907
Bme18I GGWCC 3 cut(s) 167, 409, 470
BmgT120I GGNCC 3 cut(s) 167, 409, 470
BmiI GGNNCC 2 cut(s) 54, 550
BmrFI CCNGG 6 cut(s) 95, 162, 205, 623, 800, 907
BmsI GCATC 1 cut(s) 199
BpmI CTGGAG 3 cut(s) 77, 624, 986
Bpu1102I GCTNAGC 1 cut(s) 809
BpuEI CTTGAG 2 cut(s) 247, 434
BsaJI CCNNGG 4 cut(s) 94, 204, 621, 905
BsaWI WCCGGW 2 cut(s) 55, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 823
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 2 cut(s) 817, 998
BseBI CCWGG 6 cut(s) 95, 162, 205, 623, 800, 907
BseDI CCNNGG 4 cut(s) 94, 204, 621, 905
BseGI GGATG 2 cut(s) 214, 856
BseLI CCNNNNNNNGG 2 cut(s) 100, 823
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 817, 998
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 2 cut(s) 187, 906
BshFI GGCC 4 cut(s) 116, 759, 786, 997
BshTI ACCGGT 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 798
BsiSI CCGG 2 cut(s) 56, 468
BslI CCNNNNNNNGG 2 cut(s) 100, 823
BsnI GGCC 4 cut(s) 116, 759, 786, 997
Bsp1286I GDGCHC 1 cut(s) 798
Bsp143I GATC 1 cut(s) 28
Bsp1720I GCTNAGC 1 cut(s) 809
BspACI CCGC 3 cut(s) 424, 814, 897
BspANI GGCC 4 cut(s) 116, 759, 786, 997
BspCNI CTCAG 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 54, 550
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 2 cut(s) 817, 998
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 4 cut(s) 94, 204, 621, 905
BssMI GATC 1 cut(s) 28
Bst2UI CCWGG 6 cut(s) 95, 162, 205, 623, 800, 907
Bst4CI ACNGT 2 cut(s) 397, 888
BstDEI CTNAG 2 cut(s) 107, 809
BstEII GGTNACC 1 cut(s) 681
BstF5I GGATG 2 cut(s) 214, 856
BstKTI GATC 1 cut(s) 31
BstMBI GATC 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 6 cut(s) 95, 162, 205, 623, 800, 907
BstNSI RCATGY 1 cut(s) 741
BstPI GGTNACC 1 cut(s) 681
BstSCI CCNGG 6 cut(s) 93, 160, 203, 621, 798, 905
BstV1I GCAGC 2 cut(s) 187, 906
BsuI GTATCC 1 cut(s) 752
BsuRI GGCC 4 cut(s) 116, 759, 786, 997
BtsCI GGATG 2 cut(s) 214, 856
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 167, 409, 470
CseI GACGC 1 cut(s) 374
Csp6I GTAC 4 cut(s) 4, 123, 175, 1001
CspAI ACCGGT 1 cut(s) 55
CviAII CATG 5 cut(s) 100, 197, 308, 738, 746
CviQI GTAC 4 cut(s) 4, 123, 175, 1001
DdeI CTNAG 2 cut(s) 107, 809
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
EaeI YGGCCR 3 cut(s) 114, 757, 995
Ecl136II GAGCTC 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 3 cut(s) 167, 409, 470
Eco53kI GAGCTC 1 cut(s) 796
Eco57I CTGAAG 1 cut(s) 680
Eco91I GGTNACC 1 cut(s) 681
EcoICRI GAGCTC 1 cut(s) 796
EcoO109I RGGNCCY 1 cut(s) 409
EcoO65I GGTNACC 1 cut(s) 681
EcoRII CCWGG 6 cut(s) 93, 160, 203, 621, 798, 905
EcoT38I GRGCYC 1 cut(s) 798
FaeI CATG 5 cut(s) 103, 200, 311, 741, 749
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FatI CATG 5 cut(s) 99, 196, 307, 737, 745
Fnu4HI GCNGC 4 cut(s) 201, 425, 814, 895
FokI GGATG 2 cut(s) 221, 863
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 4 cut(s) 201, 425, 814, 895
FspBI CTAG 1 cut(s) 939
GluI GCNGC 4 cut(s) 201, 425, 814, 895
GsuI CTGGAG 3 cut(s) 77, 624, 986
HaeIII GGCC 4 cut(s) 116, 759, 786, 997
HapII CCGG 2 cut(s) 56, 468
HgaI GACGC 1 cut(s) 374
Hin1II CATG 5 cut(s) 103, 200, 311, 741, 749
HinfI GANTC 1 cut(s) 462
HpaII CCGG 2 cut(s) 56, 468
HphI GGTGA 2 cut(s) 457, 581
Hpy188I TCNGA 3 cut(s) 514, 630, 826
Hpy188III TCNNGA 4 cut(s) 239, 264, 302, 446
HpyAV CCTTC 3 cut(s) 40, 625, 940
HpyCH4III ACNGT 2 cut(s) 397, 888
HpyCH4V TGCA 3 cut(s) 26, 894, 985
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 2 cut(s) 107, 809
Hsp92II CATG 5 cut(s) 103, 200, 311, 741, 749
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 4 cut(s) 69, 103, 304, 801
Lsp1109I GCAGC 2 cut(s) 187, 906
LweI GCATC 1 cut(s) 199
MaeI CTAG 1 cut(s) 939
MaeIII GTNAC 4 cut(s) 288, 463, 681, 931
MalI GATC 1 cut(s) 30
MboI GATC 1 cut(s) 28
MboII GAAGA 2 cut(s) 713, 716
MfeI CAATTG 2 cut(s) 582, 986
MhlI GDGCHC 1 cut(s) 798
MlsI TGGCCA 3 cut(s) 116, 759, 997
MluCI AATT 8 cut(s) 41, 419, 435, 553, 572, 582, 777, 986
MluNI TGGCCA 3 cut(s) 116, 759, 997
MlyI GAGTC 1 cut(s) 471
MmeI TCCRAC 1 cut(s) 828
MnlI CCTC 5 cut(s) 442, 508, 517, 570, 763
Mox20I TGGCCA 3 cut(s) 116, 759, 997
MscI TGGCCA 3 cut(s) 116, 759, 997
MseI TTAA 5 cut(s) 84, 131, 372, 666, 873
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 3 cut(s) 116, 759, 997
MspA1I CMGCKG 2 cut(s) 391, 897
MspI CCGG 2 cut(s) 56, 468
MspR9I CCNGG 6 cut(s) 95, 162, 205, 623, 800, 907
MunI CAATTG 2 cut(s) 582, 986
MvaI CCWGG 6 cut(s) 95, 162, 205, 623, 800, 907
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 1 cut(s) 28
NlaIII CATG 5 cut(s) 103, 200, 311, 741, 749
NlaIV GGNNCC 2 cut(s) 54, 550
NmuCI GTSAC 2 cut(s) 463, 931
NspI RCATGY 1 cut(s) 741
PciI ACATGT 1 cut(s) 737
PflMI CCANNNNNTGG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 798
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 4 cut(s) 202, 426, 815, 896
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PpuMI RGGWCCY 1 cut(s) 409
PscI ACATGT 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 6 cut(s) 93, 160, 203, 621, 798, 905
PspEI GGTNACC 1 cut(s) 681
PspGI CCWGG 6 cut(s) 93, 160, 203, 621, 798, 905
PspN4I GGNNCC 2 cut(s) 54, 550
PspPI GGNCC 3 cut(s) 167, 409, 470
PspPPI RGGWCCY 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 4 cut(s) 5, 124, 176, 1002
RsaNI GTAC 4 cut(s) 4, 123, 175, 1001
RseI CAYNNNNRTG 1 cut(s) 915
SacI GAGCTC 1 cut(s) 798
SaqAI TTAA 5 cut(s) 84, 131, 372, 666, 873
SatI GCNGC 4 cut(s) 201, 425, 814, 895
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 3 cut(s) 167, 409, 470
SchI GAGTC 1 cut(s) 471
ScrFI CCNGG 6 cut(s) 95, 162, 205, 623, 800, 907
SduI GDGCHC 1 cut(s) 798
SfaNI GCATC 1 cut(s) 199
SinI GGWCC 3 cut(s) 167, 409, 470
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 2 cut(s) 262, 413
SmoI CTYRAG 2 cut(s) 262, 413
Sse9I AATT 8 cut(s) 41, 419, 435, 553, 572, 582, 777, 986
SsiI CCGC 3 cut(s) 424, 814, 897
SspMI CTAG 1 cut(s) 939
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 6 cut(s) 93, 160, 203, 621, 798, 905
TaaI ACNGT 2 cut(s) 397, 888
TasI AATT 8 cut(s) 41, 419, 435, 553, 572, 582, 777, 986
TatI WGTACW 2 cut(s) 3, 122
TauI GCSGC 2 cut(s) 427, 816
Tru1I TTAA 5 cut(s) 84, 131, 372, 666, 873
Tru9I TTAA 5 cut(s) 84, 131, 372, 666, 873
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 2 cut(s) 463, 931
TseI GCWGC 2 cut(s) 200, 894
Tsp45I GTSAC 2 cut(s) 463, 931
TspDTI ATGAA 2 cut(s) 324, 358
TspRI CASTG 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 3 cut(s) 167, 409, 470
XceI RCATGY 1 cut(s) 741
XcmI CCANNNNNNNNNTGG 1 cut(s) 752
XspI CTAG 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.