Rh2CG479100

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000202
Physical Location & Seq
Reverse (-)
64403185 .. 64410112
6928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG479100.1

Sequence Viewer

Length: 2292 bp
ATGACACTGGAAGTTGCAGAGTTCTTGAGGCACAAGTTCTTGAAGTCCATTCAAGACGCAGAGTCTGAATTTTCAGGATTTGTGTTCCTCTCCCACTTCAAAGCAATCAAATGCTTCGTGGAAGAGATACAGATAATCCCGGAGATCAAAATCACTCTCGGCTCCGGCGACGACCTCTACCACAAGACCGTGTCTTTGCTCTACGAGCTCAACGACGCGTTGGCCGAATCCCGAGTATTGGCCAACGTGTGCAAGCTGCAAAATAAGAAGCTCTTTTTCTTGTTGCACCCCTTGTTGAACTACTCTATCCGAAAGATGAAGAAGCGACTCAACCAAATGAAACGCAAGTTTGCGGCTTTGGTCGAAGAGGAGAAAATGTATTCCACTCTTGTAATTTGTGATCAGACGAGTCTAGACGGTGAGCCGGGAACTGAAATGGATGATGACCGTACGAGTCAAATCGGCGGCGCAGAAACGAGTATAGTCGGTGCTGATGAGCAGGCGGAGAAGATTGAGGGCTTGCTGTGTGGCGGTGCTACGGCAATTGGGATAGTCGGCATTGCTGGCGTAGGTAAGACCACTCTAGTGCACCAGGTTTTGAATAGGCAGAGAGTGAGGGATGAGTTTTCTCCTATAATTTGGTTATGCTTATCGGACAAAGAAAAATATGAATTTACTAATAGCATCGGCGTTAGCATTGTGAGACGCATTCTTGATAAGTTAAGGGCCGTAGATAGTGGCAATGGTGCCATTGACAATCTCAATGATGCCATAGTGGAGGAGGAGAGAGATATTTCTGGCCTCGGACTTGAGAGGCTCTTGGCAAGGCTTAACCGGCTTTTATCTGGTAAAAGATATTTGATTGTGTTGGATGATGTGTGGCACATTAGCGAATTTTATTCGGATTTAGGTTGTACATTTCAGGATCGGTTATCTTATGGATTGCCTAAGGGTAGTGGTGGTGCTGTTATTGTCACCACTAGGATACCAGAAGTTGCTGAACACATGGTTGGCAGAAATAACTTGATTACTGTTGAGCCTTTGGACACAGAAAGCTGCTGGCGAATATTTATGGAGACTATCAAGGATAATAAGGAAGTTTTAAACATTTCAACTCATGAAACTTTGGATAAGATTAAAAATGAAATTAAGGACCAATGTTACGGTCTACCATTGGCTGCTAAGGAGCTCGCAGGAATCATTCCCAAACGGATTCGTGAGATAGAGTCCAACCGCTTCTTGAAGGAGATGTACATTCCTGATGAATTGCTTCATGCTGATTTGGAAGTCGAACCTGCTGTCCATATACCAAAATTCCCAGTATTGGTGTTTATTGATATGAAAAATATGAACAACAAACAGCTTGGAGAAAAACTCCTTGACAAATTTCGCTATCATCTTAATAAAAAGCAGGTTTTTGCTGTTTTAGAAGAGAAGTCTGATATGAAGAAAAAAGTAAAGGCACATGACCCTGAGAGGGTGCTAAAGGATGTTTATGGCACTTTAGAAAAGCTGAAGCGTAAAGGATATGGTTTTGCTGATGAAATTCAAAAGACGATGACGGTTATAATTGTTGGTGGTGATTCTGTGGCTAACTGGATTTTGGGAGTTATCTGTGATCTAAAATTGCCGGAGTTACCCTCCATTGCTCCAATTCCGCTGCAAACCATTTCTCTTATTGGTGGTAGCATCTCAAGTTCCTTTGGATGGAGGAGCATTTCTCCTGATGATCCAGTGAAATTGTCCCTGCTAGATGTACAATATGCAAAAAAAATGAAAACTGACAGCTGGCATATTCTCATTTGGATGAAAACCACTAAAAGTTCTACAGCTAATCAACAAATACCTCATTCTTTGCATCTATGTCATCCTGTTCACGAACCGGATATGCAAAATGCGGATAATCTCAAATTGTCCGGAGGGTTTTGGAACTACTTCAGCTTGGAAGTTGATGCTCTCCGACAATATGGAAATCACTGCTTTCATCCTACTGAAAGGACCTTATTATCAGTTGCAAATATAAAGGTTATGAAACAACTTGGTCAGTGGGATAAACTTGATATTACTAGCAGCATCAGGTCAGTCCTTTGTTTTAACTTGTCTAGACCACAAATTATGAAGAAAGATCGGAAAGAGAGATACCTGTTTCCGTCTTTCATAGATGATGGACATCTTCAAATTATTGGTTACAGAGATGTTTTCTTTCCTCTTAGTGAAGGTTCCATTCACCTTGGCCAGGTACGGGGAATTCTTTCGAGTTTATTGATGGTGAAGAAGAGGTTGTCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

763

Amino Acids

86.44

Weight (kDa)

7.07

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 162 - 306 2.9e-08 AAA ATPase domain
NB-ARC PF00931 167 - 364 1.4e-24 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1568
Acc36I ACCTGC 2 cut(s) 1303, 1402
AccB1I GGYRCC 1 cut(s) 746
AccI GTMKAC 1 cut(s) 1168
AccII CGCG 1 cut(s) 218
AccIII TCCGGA 1 cut(s) 1916
AciI CCGC 7 cut(s) 353, 465, 503, 531, 1234, 1658, 1898
AclWI GGATC 2 cut(s) 933, 1724
AcoI YGGCCR 3 cut(s) 222, 240, 2233
AcsI RAATTY 7 cut(s) 68, 671, 893, 1313, 1385, 1545, 2247
AcuI CTGAAG 2 cut(s) 1535, 1921
AfaI GTAC 5 cut(s) 451, 916, 1253, 1758, 2241
AfiI CCNNNNNNNGG 6 cut(s) 238, 1324, 1477, 1707, 2236, 2242
AflIII ACRYGT 2 cut(s) 216, 246
AgsI TTSAA 9 cut(s) 43, 53, 100, 298, 601, 1113, 1243, 1550, 2177
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 2 cut(s) 591, 2235
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
AleI CACNNNNGTG 1 cut(s) 584
AloI GAACNNNNNNTCC 2 cut(s) 1284, 1316
Alw21I GWGCWC 3 cut(s) 210, 591, 1191
Alw26I GTCTC 2 cut(s) 697, 1070
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 2 cut(s) 933, 1724
AlwNI CAGNNNCTG 1 cut(s) 65
Ama87I CYCGRG 1 cut(s) 231
Aor13HI TCCGGA 1 cut(s) 1916
AoxI GGCC 5 cut(s) 222, 240, 726, 799, 2233
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 5 cut(s) 256, 1056, 1178, 1660, 2070
ApoI RAATTY 7 cut(s) 68, 671, 893, 1313, 1385, 1545, 2247
ArsI GACNNNNNNTTYG 4 cut(s) 178, 210, 1284, 1316
Asp700I GAANNNNTTC 3 cut(s) 1269, 1934, 2251
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 3 cut(s) 726, 1153, 1998
AsuC2I CCSGG 2 cut(s) 140, 426
AsuHPI GGTGA 5 cut(s) 431, 967, 1592, 2219, 2281
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 2 cut(s) 1153, 1998
AxyI CCTNAGG 1 cut(s) 948
BaeGI GKGCMC 1 cut(s) 591
BalI TGGCCA 2 cut(s) 242, 2235
BanI GGYRCC 1 cut(s) 746
BanII GRGCYC 2 cut(s) 210, 1191
BarI GAAGNNNNNNTAC 2 cut(s) 1235, 1267
Bbv12I GWGCWC 3 cut(s) 210, 591, 1191
BbvI GCAGC 5 cut(s) 243, 1043, 1165, 1647, 2082
BccI CCATC 3 cut(s) 1701, 2159, 2260
BceAI ACGGC 2 cut(s) 555, 713
BciT130I CCWGG 2 cut(s) 593, 2237
BciVI GTATCC 1 cut(s) 978
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 2 cut(s) 140, 426
BcoDI GTCTC 2 cut(s) 697, 1070
BfaI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
BfmI CTRYAG 1 cut(s) 1827
BfuAI ACCTGC 2 cut(s) 1303, 1402
BfuI GTATCC 1 cut(s) 978
BisI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 1661, 2071
BlsI GCNGC 7 cut(s) 258, 355, 467, 1058, 1180, 1662, 2072
Bme1390I CCNGG 4 cut(s) 140, 426, 593, 2237
Bme18I GGWCC 2 cut(s) 1153, 1998
BmeRI GACNNNNNGTC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 3 cut(s) 726, 1153, 1998
BmiI GGNNCC 3 cut(s) 163, 748, 2221
BmrFI CCNGG 4 cut(s) 140, 426, 593, 2237
BmrI ACTGGG 1 cut(s) 1313
BmsI GCATC 6 cut(s) 693, 757, 1698, 1867, 1942, 2082
BmuI ACTGGG 1 cut(s) 1313
BplI GAGNNNNNCTC 6 cut(s) 1359, 1391, 1625, 1657, 1705, 1737
Bpu10I CCTNAGC 1 cut(s) 1182
BpuEI CTTGAG 3 cut(s) 46, 830, 1678
BpuMI CCSGG 2 cut(s) 140, 426
BsaBI GATNNNNATC 2 cut(s) 149, 2169
BsaJI CCNNGG 2 cut(s) 802, 2230
BsaWI WCCGGW 2 cut(s) 1882, 1916
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 1324, 1477, 1707, 2236, 2242
Bse118I RCCGGY 1 cut(s) 834
Bse1I ACTGG 4 cut(s) 12, 1319, 1601, 1733
Bse21I CCTNAGG 1 cut(s) 948
Bse3DI GCAATG 3 cut(s) 558, 748, 1644
Bse8I GATNNNNATC 2 cut(s) 149, 2169
BseAI TCCGGA 1 cut(s) 1916
BseBI CCWGG 2 cut(s) 593, 2237
BseDI CCNNGG 2 cut(s) 802, 2230
BseGI GGATG 8 cut(s) 445, 625, 877, 1495, 1712, 1812, 1867, 1984
BseJI GATNNNNATC 2 cut(s) 149, 2169
BseLI CCNNNNNNNGG 6 cut(s) 238, 1324, 1477, 1707, 2236, 2242
BseMI GCAATG 3 cut(s) 558, 748, 1644
BseMII CTCAG 1 cut(s) 1464
BseNI ACTGG 4 cut(s) 12, 1319, 1601, 1733
BseRI GAGGAG 4 cut(s) 383, 794, 797, 1726
BseSI GKGCMC 1 cut(s) 591
BseXI GCAGC 5 cut(s) 243, 1043, 1165, 1647, 2082
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BshNI GGYRCC 1 cut(s) 746
BsiHKAI GWGCWC 3 cut(s) 210, 591, 1191
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 7 cut(s) 140, 165, 425, 835, 1631, 1883, 1917
BsiWI CGTACG 1 cut(s) 449
BslFI GGGAC 1 cut(s) 1729
BslI CCNNNNNNNGG 6 cut(s) 238, 1324, 1477, 1707, 2236, 2242
BsmAI GTCTC 2 cut(s) 697, 1070
BsmBI CGTCTC 1 cut(s) 697
BsmFI GGGAC 1 cut(s) 1729
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BsoBI CYCGRG 1 cut(s) 231
Bsp1286I GDGCHC 3 cut(s) 210, 591, 1191
Bsp13I TCCGGA 1 cut(s) 1916
Bsp1407I TGTACA 3 cut(s) 914, 1251, 1756
Bsp143I GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
BspACI CCGC 7 cut(s) 353, 465, 503, 531, 1234, 1658, 1898
BspANI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BspCNI CTCAG 1 cut(s) 1465
BspEI TCCGGA 1 cut(s) 1916
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 3 cut(s) 163, 748, 2221
BspMI ACCTGC 2 cut(s) 1303, 1402
BspPI GGATC 2 cut(s) 933, 1724
BspT107I GGYRCC 1 cut(s) 746
BsrDI GCAATG 3 cut(s) 558, 748, 1644
BsrFI RCCGGY 1 cut(s) 834
BsrGI TGTACA 3 cut(s) 914, 1251, 1756
BsrI ACTGG 4 cut(s) 12, 1319, 1601, 1733
BssAI RCCGGY 1 cut(s) 834
BssECI CCNNGG 2 cut(s) 802, 2230
BssMI GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
BssT1I CCWWGG 1 cut(s) 2230
Bst2UI CCWGG 2 cut(s) 593, 2237
Bst4CI ACNGT 6 cut(s) 190, 419, 449, 1033, 1166, 1564
Bst6I CTCTTC 4 cut(s) 117, 360, 1425, 2270
BstAUI TGTACA 3 cut(s) 914, 1251, 1756
BstC8I GCNNGC 7 cut(s) 254, 501, 521, 565, 1061, 1191, 1790
BstDEI CTNAG 4 cut(s) 948, 1182, 1473, 2210
BstENI CCTNNNNNAGG 1 cut(s) 2234
BstF5I GGATG 8 cut(s) 445, 625, 877, 1495, 1712, 1812, 1867, 1984
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 6 cut(s) 147, 403, 928, 1621, 1732, 2128
BstMAI GTCTC 2 cut(s) 697, 1070
BstMBI GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
BstMWI GCNNNNNNNGC 3 cut(s) 205, 564, 835
BstNI CCWGG 2 cut(s) 593, 2237
BstSCI CCNGG 4 cut(s) 138, 424, 591, 2235
BstSFI CTRYAG 1 cut(s) 1827
BstSLI GKGCMC 1 cut(s) 591
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 5 cut(s) 243, 1043, 1165, 1647, 2082
Bsu36I CCTNAGG 1 cut(s) 948
BsuI GTATCC 1 cut(s) 978
BsuRI GGCC 5 cut(s) 224, 242, 728, 801, 2235
BtsCI GGATG 8 cut(s) 445, 625, 877, 1495, 1712, 1812, 1867, 1984
BtsI GCAGTG 1 cut(s) 1975
BtsIMutI CAGTG 4 cut(s) 5, 1740, 1975, 2051
BveI ACCTGC 2 cut(s) 1303, 1402
Cac8I GCNNGC 7 cut(s) 254, 501, 521, 565, 1061, 1191, 1790
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 1 cut(s) 1117
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 1 cut(s) 834
Cfr13I GGNCC 3 cut(s) 726, 1153, 1998
CseI GACGC 3 cut(s) 65, 224, 714
CsiI ACCWGGT 1 cut(s) 591
Csp6I GTAC 5 cut(s) 450, 915, 1252, 1757, 2240
CviAII CATG 5 cut(s) 1006, 1118, 1274, 1466, 2289
CviQI GTAC 5 cut(s) 450, 915, 1252, 1757, 2240
DdeI CTNAG 4 cut(s) 948, 1182, 1473, 2210
DpnI GATC 6 cut(s) 146, 402, 927, 1620, 1731, 2127
DpnII GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
DraI TTTAAA 1 cut(s) 1104
DriI GACNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 3 cut(s) 222, 240, 2233
Eam1104I CTCTTC 4 cut(s) 117, 360, 1425, 2270
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 4 cut(s) 117, 360, 1425, 2270
EciI GGCGGA 1 cut(s) 518
Ecl136II GAGCTC 2 cut(s) 208, 1189
Eco130I CCWWGG 1 cut(s) 2230
Eco24I GRGCYC 2 cut(s) 210, 1191
Eco47I GGWCC 2 cut(s) 1153, 1998
Eco53kI GAGCTC 2 cut(s) 208, 1189
Eco57I CTGAAG 2 cut(s) 1535, 1921
Eco81I CCTNAGG 1 cut(s) 948
Eco88I CYCGRG 1 cut(s) 231
EcoICRI GAGCTC 2 cut(s) 208, 1189
EcoNI CCTNNNNNAGG 1 cut(s) 2234
EcoO109I RGGNCCY 1 cut(s) 1998
EcoRI GAATTC 1 cut(s) 2247
EcoRII CCWGG 2 cut(s) 591, 2235
EcoT14I CCWWGG 1 cut(s) 2230
EcoT38I GRGCYC 2 cut(s) 210, 1191
ErhI CCWWGG 1 cut(s) 2230
Esp3I CGTCTC 1 cut(s) 697
FaeI CATG 5 cut(s) 1009, 1121, 1277, 1469, 2292
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 1729
FatI CATG 5 cut(s) 1005, 1117, 1273, 1465, 2288
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 1661, 2071
FokI GGATG 8 cut(s) 452, 632, 884, 1502, 1719, 1819, 1854, 1971
FriOI GRGCYC 2 cut(s) 210, 1191
Fsp4HI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 1661, 2071
FspBI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
GlaI GCGC 1 cut(s) 469
GluI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 1661, 2071
HaeIII GGCC 5 cut(s) 224, 242, 728, 801, 2235
HapII CCGG 7 cut(s) 140, 165, 425, 835, 1631, 1883, 1917
HgaI GACGC 3 cut(s) 65, 224, 714
HhaI GCGC 1 cut(s) 470
Hin1II CATG 5 cut(s) 1009, 1121, 1277, 1469, 2292
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 1 cut(s) 2286
HindII GTYRAC 1 cut(s) 2286
HinfI GANTC 9 cut(s) 62, 227, 327, 409, 454, 1197, 1213, 1226, 1583
HpaII CCGG 7 cut(s) 140, 165, 425, 835, 1631, 1883, 1917
HphI GGTGA 5 cut(s) 431, 967, 1592, 2219, 2281
Hpy166II GTNNAC 4 cut(s) 589, 1169, 1876, 2286
Hpy188I TCNGA 9 cut(s) 67, 311, 405, 655, 806, 904, 1441, 1961, 2130
Hpy8I GTNNAC 4 cut(s) 589, 1169, 1876, 2286
Hpy99I CGWCG 2 cut(s) 173, 218
HpyAV CCTTC 2 cut(s) 1237, 2210
HpyCH4III ACNGT 6 cut(s) 190, 419, 449, 1033, 1166, 1564
HpyCH4IV ACGT 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 564, 835
HpyF3I CTNAG 4 cut(s) 948, 1182, 1473, 2210
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 5 cut(s) 1009, 1121, 1277, 1469, 2292
HspAI GCGC 1 cut(s) 468
Kpn2I TCCGGA 1 cut(s) 1916
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
LmnI GCTCC 4 cut(s) 167, 1186, 1654, 1713
Lsp1109I GCAGC 5 cut(s) 243, 1043, 1165, 1647, 2082
LweI GCATC 6 cut(s) 693, 757, 1698, 1867, 1942, 2082
MabI ACCWGGT 1 cut(s) 591
MaeI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 4 cut(s) 973, 1160, 1635, 2186
MalI GATC 6 cut(s) 146, 402, 927, 1620, 1731, 2127
MboI GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
MfeI CAATTG 1 cut(s) 543
MhlI GDGCHC 3 cut(s) 210, 591, 1191
MlsI TGGCCA 2 cut(s) 242, 2235
MluI ACGCGT 1 cut(s) 216
MluNI TGGCCA 2 cut(s) 242, 2235
MlyI GAGTC 5 cut(s) 71, 321, 418, 463, 1235
MmeI TCCRAC 3 cut(s) 849, 1254, 1984
Mox20I TGGCCA 2 cut(s) 242, 2235
MroI TCCGGA 1 cut(s) 1916
MroXI GAANNNNTTC 3 cut(s) 1269, 1934, 2251
MscI TGGCCA 2 cut(s) 242, 2235
MseI TTAA 7 cut(s) 722, 831, 1103, 1137, 1149, 1401, 2094
MslI CAYNNNNRTG 2 cut(s) 584, 1805
Msp20I TGGCCA 2 cut(s) 242, 2235
MspA1I CMGCKG 2 cut(s) 1660, 1788
MspI CCGG 7 cut(s) 140, 165, 425, 835, 1631, 1883, 1917
MspR9I CCNGG 4 cut(s) 140, 426, 593, 2237
MunI CAATTG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 2 cut(s) 593, 2237
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 3 cut(s) 205, 564, 835
NciI CCSGG 2 cut(s) 140, 426
NdeII GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
NlaIII CATG 5 cut(s) 1009, 1121, 1277, 1469, 2292
NlaIV GGNNCC 3 cut(s) 163, 748, 2221
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 973
OliI CACNNNNGTG 1 cut(s) 584
PagI TCATGA 1 cut(s) 1117
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 210, 222
PctI GAATGC 1 cut(s) 708
PdmI GAANNNNTTC 3 cut(s) 1269, 1934, 2251
PfeI GAWTC 4 cut(s) 227, 1197, 1213, 1583
Pfl23II CGTACG 1 cut(s) 449
PflFI GACNNNGTC 1 cut(s) 190
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 7 cut(s) 258, 355, 467, 1058, 1180, 1662, 2072
PleI GAGTC 5 cut(s) 70, 321, 417, 462, 1234
PpsI GAGTC 5 cut(s) 70, 321, 417, 462, 1234
PpuMI RGGWCCY 1 cut(s) 1998
PsiI TTATAA 1 cut(s) 1568
Psp124BI GAGCTC 2 cut(s) 210, 1191
Psp5II RGGWCCY 1 cut(s) 1998
Psp6I CCWGG 2 cut(s) 591, 2235
PspGI CCWGG 2 cut(s) 591, 2235
PspLI CGTACG 1 cut(s) 449
PspN4I GGNNCC 3 cut(s) 163, 748, 2221
PspPI GGNCC 3 cut(s) 726, 1153, 1998
PspPPI RGGWCCY 1 cut(s) 1998
PstNI CAGNNNCTG 1 cut(s) 65
PsyI GACNNNGTC 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 1788
RsaI GTAC 5 cut(s) 451, 916, 1253, 1758, 2241
RsaNI GTAC 5 cut(s) 450, 915, 1252, 1757, 2240
RseI CAYNNNNRTG 2 cut(s) 584, 1805
SacI GAGCTC 2 cut(s) 210, 1191
SaqAI TTAA 7 cut(s) 722, 831, 1103, 1137, 1149, 1401, 2094
SatI GCNGC 7 cut(s) 257, 354, 466, 1057, 1179, 1661, 2071
Sau3AI GATC 6 cut(s) 144, 400, 925, 1618, 1729, 2125
Sau96I GGNCC 3 cut(s) 726, 1153, 1998
SchI GAGTC 5 cut(s) 71, 321, 418, 463, 1235
ScrFI CCNGG 4 cut(s) 140, 426, 593, 2237
SduI GDGCHC 3 cut(s) 210, 591, 1191
SexAI ACCWGGT 1 cut(s) 591
SfaNI GCATC 6 cut(s) 693, 757, 1698, 1867, 1942, 2082
SfcI CTRYAG 1 cut(s) 1827
SinI GGWCC 2 cut(s) 1153, 1998
SmiMI CAYNNNNRTG 2 cut(s) 584, 1805
SmlI CTYRAG 3 cut(s) 25, 809, 1693
SmoI CTYRAG 3 cut(s) 25, 809, 1693
SsiI CCGC 7 cut(s) 353, 465, 503, 531, 1234, 1658, 1898
SspI AATATT 1 cut(s) 1068
SspMI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
SstI GAGCTC 2 cut(s) 210, 1191
StyD4I CCNGG 4 cut(s) 138, 424, 591, 2235
StyI CCWWGG 1 cut(s) 2230
TaaI ACNGT 6 cut(s) 190, 419, 449, 1033, 1166, 1564
TaiI ACGT 1 cut(s) 249
TaqI TCGA 3 cut(s) 363, 1290, 2255
TatI WGTACW 3 cut(s) 914, 1251, 1756
TauI GCSGC 2 cut(s) 356, 468
TfiI GAWTC 4 cut(s) 227, 1197, 1213, 1583
Tru1I TTAA 7 cut(s) 722, 831, 1103, 1137, 1149, 1401, 2094
Tru9I TTAA 7 cut(s) 722, 831, 1103, 1137, 1149, 1401, 2094
TscAI CASTG 4 cut(s) 12, 1740, 1982, 2051
TseFI GTSAC 1 cut(s) 973
TseI GCWGC 5 cut(s) 256, 1056, 1178, 1660, 2070
Tsp45I GTSAC 1 cut(s) 973
TspGWI ACGGA 2 cut(s) 1225, 2139
TspRI CASTG 4 cut(s) 12, 1740, 1982, 2051
Tth111I GACNNNGTC 1 cut(s) 190
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 2 cut(s) 1153, 1998
XagI CCTNNNNNAGG 1 cut(s) 2234
XapI RAATTY 7 cut(s) 68, 671, 893, 1313, 1385, 1545, 2247
XbaI TCTAGA 2 cut(s) 412, 2102
XmiI GTMKAC 1 cut(s) 1168
XmnI GAANNNNTTC 3 cut(s) 1269, 1934, 2251
XspI CTAG 6 cut(s) 413, 584, 981, 1751, 2067, 2103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.