Rh7CG427500

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000202
Physical Location & Seq
Forward (+)
55750132 .. 55751124
993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG427500.1

Sequence Viewer

Length: 993 bp
ATGGAGCTTAATAATGTGATCTCACGCTTCAACTTTCGAAGATTTACTTTTGTCGCAAAGGTAAAGAGTCGGTTGACCAAATTAAAGCAGGAGCTTGAGCACTTGCCGCAACAACCAATTGGCAATCCTCGGACAAAGACCGAGTTCACCGGATCCGGACCTAGAGTACCTTCAGCCAAAGTTTTCGGATTTGATGAGCAATTAAAGGAGGTTAATGAGGAGTTGCTACAAGCAGGGGTTTCCAGTTCTAGCAGTGCTGGTGAACTGAGGGCAATTGGGGTAGTTGGAATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTGGAGAGCAAGGAAGTGAAGCAGAAGTTTAATCATATACTTTGGTTACCCTTTTCGTTGAGTGTGGAAGAAGAACAATATAGTAAGTTCAGCTTTGAGACACGTATCTTTGATAGTAATGGCCAAATCATGGAGGCGAAGATTGTTGGCCTTGATAAGCTCCTGGAAAGGCTAAGCCGCCTGCTGTTATCGGATAAATGCTATTTGATTGTGTTGGATGATGTGTGTCATCGGCACATTAACATTATGGAACGCTTATGCAGCGGATTGCCCAAGCATAATGGTGGTGTTGTCATTGTCACTACTAGATTGAAGGAAGTGGCTCAAAAGCTGGGGAAACAACATAGATTGCATCTGGTTCATGTTAAGCCTCTGGAAAGAGAGATTTGCGGGTGTATATTTGAGGAGCAGGCTTATTCCATTAGAAAAAGTTCAAACCTCTCACATGATGAGGCTACGAGAAAGATGGAGGAACTCAAGGATCAGTGCCATGGTCTACCGTTGGTTGCGAAGACGATAGCAAATGCTTTTGCAGTGGGATTTGGAGGCGACGGATCTGAAGAGAGAGATGGAGATCACGGGGAGGAAGTAGGAACTTTTGAAGAATTAGTTGAAGACGATTCAAGAATTGCTGACCTGACCCATCATGTTCAAGAATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

330

Amino Acids

37.02

Weight (kDa)

6.37

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 86 - 247 3.6e-22 NB-ARC domain
NACHT PF05729 92 - 237 7.1e-06 NACHT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 457
AccI GTMKAC 1 cut(s) 823
AccIII TCCGGA 1 cut(s) 155
AciI CCGC 4 cut(s) 107, 505, 591, 717
AclWI GGATC 4 cut(s) 147, 160, 816, 889
AcoI YGGCCR 1 cut(s) 448
AcuI CTGAAG 2 cut(s) 156, 906
AfaI GTAC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 457
AflIII ACRYGT 1 cut(s) 428
AgsI TTSAA 7 cut(s) 31, 640, 762, 929, 941, 951, 980
AjnI CCWGG 2 cut(s) 312, 489
AluBI AGCT 5 cut(s) 7, 94, 420, 487, 658
AluI AGCT 5 cut(s) 7, 94, 420, 487, 658
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 4 cut(s) 147, 160, 816, 889
Aor13HI TCCGGA 1 cut(s) 155
AoxI GGCC 2 cut(s) 448, 475
ApeKI GCWGC 1 cut(s) 588
Asp700I GAANNNNTTC 1 cut(s) 757
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 139, 272
AsuII TTCGAA 1 cut(s) 37
AvaII GGWCC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 450
BamHI GGATCC 1 cut(s) 152
BbsI GAAGAC 2 cut(s) 845, 948
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 600
BccI CCATC 3 cut(s) 787, 890, 978
BciT130I CCWGG 2 cut(s) 314, 491
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 3 cut(s) 162, 249, 633
BisI GCNGC 3 cut(s) 107, 505, 589
BlpI GCTNAGC 1 cut(s) 500
BlsI GCNGC 3 cut(s) 108, 506, 590
Bme1390I CCNGG 2 cut(s) 314, 491
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 2 cut(s) 314, 491
BmsI GCATC 1 cut(s) 688
BpiI GAAGAC 2 cut(s) 845, 948
BpmI CTGGAG 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 37
BpuEI CTTGAG 2 cut(s) 116, 788
BsaAI YACGTR 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 900
BsaJI CCNNGG 3 cut(s) 128, 312, 817
BsaWI WCCGGW 2 cut(s) 149, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 243
Bse8I GATNNNNATC 1 cut(s) 900
BseAI TCCGGA 1 cut(s) 155
BseBI CCWGG 2 cut(s) 314, 491
BseDI CCNNGG 3 cut(s) 128, 312, 817
BseGI GGATG 1 cut(s) 550
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 243
BseRI GAGGAG 2 cut(s) 233, 746
BseXI GCAGC 1 cut(s) 600
BseYI CCCAGC 1 cut(s) 658
BshFI GGCC 2 cut(s) 450, 477
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 150, 156
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 419
BsnI GGCC 2 cut(s) 450, 477
Bsp119I TTCGAA 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 102
Bsp13I TCCGGA 1 cut(s) 155
Bsp143I GATC 5 cut(s) 18, 152, 808, 881, 901
Bsp1720I GCTNAGC 1 cut(s) 500
Bsp19I CCATGG 1 cut(s) 817
BspACI CCGC 4 cut(s) 107, 505, 591, 717
BspANI GGCC 2 cut(s) 450, 477
BspCNI CTCAG 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 154
BspPI GGATC 4 cut(s) 147, 160, 816, 889
BspT104I TTCGAA 1 cut(s) 37
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 3 cut(s) 128, 312, 817
BssMI GATC 5 cut(s) 18, 152, 808, 881, 901
BssT1I CCWWGG 1 cut(s) 817
Bst2UI CCWGG 2 cut(s) 314, 491
Bst4CI ACNGT 1 cut(s) 828
Bst6I CTCTTC 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 509, 738
BstDEI CTNAG 2 cut(s) 266, 500
BstDSI CCRYGG 1 cut(s) 817
BstEII GGTNACC 1 cut(s) 372
BstF5I GGATG 1 cut(s) 550
BstKTI GATC 5 cut(s) 21, 155, 811, 884, 904
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 5 cut(s) 18, 152, 808, 881, 901
BstMWI GCNNNNNNNGC 2 cut(s) 106, 588
BstNI CCWGG 2 cut(s) 314, 491
BstPI GGTNACC 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 312, 489
BstV1I GCAGC 1 cut(s) 600
BstV2I GAAGAC 2 cut(s) 845, 948
BstX2I RGATCY 2 cut(s) 152, 881
BstYI RGATCY 2 cut(s) 152, 881
BsuRI GGCC 2 cut(s) 450, 477
BtgI CCRYGG 1 cut(s) 817
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 2 cut(s) 259, 867
BtsIMutI CAGTG 3 cut(s) 259, 818, 867
Cac8I GCNNGC 2 cut(s) 509, 738
Cfr13I GGNCC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 167
CviAII CATG 5 cut(s) 457, 689, 773, 818, 974
CviQI GTAC 1 cut(s) 167
DdeI CTNAG 2 cut(s) 266, 500
DpnI GATC 5 cut(s) 20, 154, 810, 883, 903
DpnII GATC 5 cut(s) 18, 152, 808, 881, 901
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Eco130I CCWWGG 1 cut(s) 817
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 2 cut(s) 156, 906
Eco91I GGTNACC 1 cut(s) 372
EcoO65I GGTNACC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 312, 489
EcoT14I CCWWGG 1 cut(s) 817
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 5 cut(s) 460, 692, 776, 821, 977
FalI AAGNNNNNCTT 4 cut(s) 31, 63, 404, 436
FatI CATG 5 cut(s) 456, 688, 772, 817, 973
FauI CCCGC 1 cut(s) 710
FblI GTMKAC 1 cut(s) 823
Fnu4HI GCNGC 3 cut(s) 107, 505, 589
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 3 cut(s) 107, 505, 589
FspBI CTAG 3 cut(s) 162, 249, 633
GluI GCNGC 3 cut(s) 107, 505, 589
GsaI CCCAGC 1 cut(s) 662
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 2 cut(s) 450, 477
HapII CCGG 2 cut(s) 150, 156
Hin1II CATG 5 cut(s) 460, 692, 776, 821, 977
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 3 cut(s) 67, 947, 983
HpaII CCGG 2 cut(s) 150, 156
HphI GGTGA 2 cut(s) 139, 272
Hpy166II GTNNAC 4 cut(s) 75, 147, 263, 824
Hpy188I TCNGA 5 cut(s) 132, 188, 321, 520, 886
Hpy188III TCNNGA 5 cut(s) 156, 701, 951, 980, 990
Hpy8I GTNNAC 4 cut(s) 75, 147, 263, 824
Hpy99I CGWCG 1 cut(s) 881
HpyAV CCTTC 3 cut(s) 180, 316, 634
HpyCH4III ACNGT 1 cut(s) 828
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 3 cut(s) 588, 679, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 588
HpyF3I CTNAG 2 cut(s) 266, 500
HpySE526I ACGT 1 cut(s) 430
Hsp92II CATG 5 cut(s) 460, 692, 776, 821, 977
Kpn2I TCCGGA 1 cut(s) 155
Kzo9I GATC 5 cut(s) 18, 152, 808, 881, 901
LmnI GCTCC 4 cut(s) 4, 91, 492, 733
Lsp1109I GCAGC 1 cut(s) 600
LweI GCATC 1 cut(s) 688
MaeI CTAG 3 cut(s) 162, 249, 633
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 2 cut(s) 372, 625
MalI GATC 5 cut(s) 20, 154, 810, 883, 903
MboI GATC 5 cut(s) 18, 152, 808, 881, 901
MboII GAAGA 9 cut(s) 51, 407, 410, 478, 850, 899, 941, 953, 978
MfeI CAATTG 2 cut(s) 117, 273
MflI RGATCY 2 cut(s) 152, 881
MhlI GDGCHC 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 450
MluCI AATT 7 cut(s) 80, 117, 200, 273, 288, 932, 954
MluNI TGGCCA 1 cut(s) 450
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 265, 522
Mox20I TGGCCA 1 cut(s) 450
MroI TCCGGA 1 cut(s) 155
MroXI GAANNNNTTC 1 cut(s) 757
MscI TGGCCA 1 cut(s) 450
MseI TTAA 7 cut(s) 9, 83, 203, 213, 357, 567, 693
MslI CAYNNNNRTG 1 cut(s) 609
Msp20I TGGCCA 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 591
MspI CCGG 2 cut(s) 150, 156
MspR9I CCNGG 2 cut(s) 314, 491
MunI CAATTG 2 cut(s) 117, 273
MvaI CCWGG 2 cut(s) 314, 491
MwoI GCNNNNNNNGC 2 cut(s) 106, 588
NcoI CCATGG 1 cut(s) 817
NdeII GATC 5 cut(s) 18, 152, 808, 881, 901
NlaIII CATG 5 cut(s) 460, 692, 776, 821, 977
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 625
NspV TTCGAA 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 757
PfeI GAWTC 2 cut(s) 947, 983
PflMI CCANNNNNTGG 1 cut(s) 457
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 3 cut(s) 108, 506, 590
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
Ppu21I YACGTR 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 312, 489
PspEI GGTNACC 1 cut(s) 372
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 2 cut(s) 312, 489
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 2 cut(s) 152, 881
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
RseI CAYNNNNRTG 1 cut(s) 609
SaqAI TTAA 7 cut(s) 9, 83, 203, 213, 357, 567, 693
SatI GCNGC 3 cut(s) 107, 505, 589
Sau3AI GATC 5 cut(s) 18, 152, 808, 881, 901
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 2 cut(s) 314, 491
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 1 cut(s) 688
SfuI TTCGAA 1 cut(s) 37
SinI GGWCC 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 2 cut(s) 95, 803
SmoI CTYRAG 2 cut(s) 95, 803
Sse9I AATT 7 cut(s) 80, 117, 200, 273, 288, 932, 954
SsiI CCGC 4 cut(s) 107, 505, 591, 717
SspMI CTAG 3 cut(s) 162, 249, 633
StyD4I CCNGG 2 cut(s) 312, 489
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 1 cut(s) 828
TaiI ACGT 1 cut(s) 433
TaqI TCGA 1 cut(s) 37
TaqII GACCGA 1 cut(s) 155
TasI AATT 7 cut(s) 80, 117, 200, 273, 288, 932, 954
TauI GCSGC 2 cut(s) 109, 507
TfiI GAWTC 2 cut(s) 947, 983
Tru1I TTAA 7 cut(s) 9, 83, 203, 213, 357, 567, 693
Tru9I TTAA 7 cut(s) 9, 83, 203, 213, 357, 567, 693
TscAI CASTG 3 cut(s) 259, 818, 867
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 1 cut(s) 588
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 1 cut(s) 677
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 3 cut(s) 259, 818, 867
Van91I CCANNNNNTGG 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 158
XmiI GTMKAC 1 cut(s) 823
XmnI GAANNNNTTC 1 cut(s) 757
XspI CTAG 3 cut(s) 162, 249, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.