Rmu_sc0034442.1_g000001

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0034442.1
OG0000202
Physical Location & Seq
Reverse (-)
1615 .. 9733
8119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0034442.1_g000001.1.cds

Sequence Viewer

Length: 1002 bp
atggttaatagtgtagctgaatcttttaatgcttggtttgctgttgagcgtgagatgccagtatacactatgcttgatcagactcagattagggagcttgagcacttgccgcaacaaccaattggcaatcctcggacaaagaccgaattcaccggatccggacctaaagtaccttcggccaaagttttcggatttgatgagcaattaaaggagattagtgaggagttgctacaagcagggggttccagttttagcagtgctggtgaactgagggcaattggggtagttggaattgctggagtgggtaagaccaccctggttcagaaggttttggagagcaaggaagtgaagcagaagtttaatcatatactttggttgcccttttcgttgagtgaggaagaagaacaatatggtaagttcagctttgagacacgtatctttgatagtaatggccaaatcatggaggcgaggattgttggccttgataagctcctggaaaggctaagccgcctgctgttatcggataaatgctatttgattgtgttggatgatgtgtgtcatcggcacattaacattatggaacgcttatgcagcggattgcccaagcataatggtggtgttgtcattgtcactactagattgaaggaagtggctcaaaagctggggaaacaacatagattgcatctggttcatgttaagcctctggacagagagatttgcgggcatatatttgaggagcaggcttattccattagaaaaagttcaaacttctcaggtgatgaagctacgagaaagatggaggaactcaaggatcagtgccatggtctaccgttggttgcgaagacgatagcaaatgcttttgcagtgggatttggaggcgacggatctgaacagagaggtggtgatcacggggaggaagtaggaatttttgaagaattagttgaagacgaccagggttcaagaattgctgacctgatccatcatgttcaagaatcttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

333

Amino Acids

37.14

Weight (kDa)

5.59

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 460
AccI GTMKAC 2 cut(s) 63, 826
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 4 cut(s) 110, 508, 594, 720
AclWI GGATC 5 cut(s) 150, 163, 819, 892, 970
AcoI YGGCCR 2 cut(s) 177, 451
AcsI RAATTY 2 cut(s) 146, 924
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 460
AflIII ACRYGT 1 cut(s) 431
AgsI TTSAA 6 cut(s) 643, 765, 932, 944, 960, 989
AjnI CCWGG 3 cut(s) 315, 492, 951
AluBI AGCT 6 cut(s) 17, 97, 423, 490, 661, 785
AluI AGCT 6 cut(s) 17, 97, 423, 490, 661, 785
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 5 cut(s) 150, 163, 819, 892, 970
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 3 cut(s) 177, 451, 478
ApeKI GCWGC 1 cut(s) 591
ApoI RAATTY 2 cut(s) 146, 924
Asp700I GAANNNNTTC 1 cut(s) 760
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 4 cut(s) 142, 275, 788, 914
AvaII GGWCC 1 cut(s) 161
BalI TGGCCA 1 cut(s) 453
BamHI GGATCC 1 cut(s) 155
BbsI GAAGAC 2 cut(s) 848, 951
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 603
BccI CCATC 2 cut(s) 790, 987
BciT130I CCWGG 3 cut(s) 317, 494, 953
BclI TGATCA 2 cut(s) 76, 904
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 1 cut(s) 636
BisI GCNGC 3 cut(s) 110, 508, 592
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 3 cut(s) 111, 509, 593
Bme1390I CCNGG 3 cut(s) 317, 494, 953
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 157, 244
BmrFI CCNGG 3 cut(s) 317, 494, 953
BmsI GCATC 2 cut(s) 45, 691
BpiI GAAGAC 2 cut(s) 848, 951
BpmI CTGGAG 1 cut(s) 318
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 119, 791
BsaAI YACGTR 1 cut(s) 434
BsaJI CCNNGG 4 cut(s) 131, 315, 820, 952
BsaWI WCCGGW 2 cut(s) 152, 158
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 59, 246
BseAI TCCGGA 1 cut(s) 158
BseBI CCWGG 3 cut(s) 317, 494, 953
BseDI CCNNGG 4 cut(s) 131, 315, 820, 952
BseGI GGATG 1 cut(s) 553
BseLI CCNNNNNNNGG 1 cut(s) 460
BseMII CTCAG 3 cut(s) 98, 260, 786
BseNI ACTGG 2 cut(s) 59, 246
BseRI GAGGAG 2 cut(s) 236, 749
BseXI GCAGC 1 cut(s) 603
BseYI CCCAGC 1 cut(s) 661
BshFI GGCC 3 cut(s) 179, 453, 480
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 2 cut(s) 153, 159
BslI CCNNNNNNNGG 1 cut(s) 460
BsmAI GTCTC 1 cut(s) 422
BsnI GGCC 3 cut(s) 179, 453, 480
Bsp1286I GDGCHC 1 cut(s) 105
Bsp13I TCCGGA 1 cut(s) 158
Bsp143I GATC 6 cut(s) 76, 155, 811, 884, 904, 975
Bsp1720I GCTNAGC 1 cut(s) 503
Bsp19I CCATGG 1 cut(s) 820
BspACI CCGC 4 cut(s) 110, 508, 594, 720
BspANI GGCC 3 cut(s) 179, 453, 480
BspCNI CTCAG 3 cut(s) 97, 261, 785
BspEI TCCGGA 1 cut(s) 158
BspLI GGNNCC 2 cut(s) 157, 244
BspPI GGATC 5 cut(s) 150, 163, 819, 892, 970
BsrI ACTGG 2 cut(s) 59, 246
BssECI CCNNGG 4 cut(s) 131, 315, 820, 952
BssMI GATC 6 cut(s) 76, 155, 811, 884, 904, 975
BssNAI GTATAC 1 cut(s) 64
BssT1I CCWWGG 1 cut(s) 820
Bst1107I GTATAC 1 cut(s) 64
Bst2UI CCWGG 3 cut(s) 317, 494, 953
Bst4CI ACNGT 1 cut(s) 831
BstBAI YACGTR 1 cut(s) 434
BstC8I GCNNGC 3 cut(s) 512, 722, 741
BstDEI CTNAG 4 cut(s) 84, 269, 503, 772
BstDSI CCRYGG 1 cut(s) 820
BstF5I GGATG 1 cut(s) 553
BstKTI GATC 6 cut(s) 79, 158, 814, 887, 907, 978
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 6 cut(s) 76, 155, 811, 884, 904, 975
BstMWI GCNNNNNNNGC 4 cut(s) 38, 55, 109, 591
BstNI CCWGG 3 cut(s) 317, 494, 953
BstSCI CCNGG 3 cut(s) 315, 492, 951
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 2 cut(s) 848, 951
BstX2I RGATCY 2 cut(s) 155, 884
BstYI RGATCY 2 cut(s) 155, 884
BstZ17I GTATAC 1 cut(s) 64
BsuRI GGCC 3 cut(s) 179, 453, 480
BtgI CCRYGG 1 cut(s) 820
BtsCI GGATG 1 cut(s) 553
BtsI GCAGTG 2 cut(s) 262, 870
BtsIMutI CAGTG 3 cut(s) 262, 821, 870
Cac8I GCNNGC 3 cut(s) 512, 722, 741
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 170
CviAII CATG 4 cut(s) 460, 692, 821, 983
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 4 cut(s) 84, 269, 503, 772
DpnI GATC 6 cut(s) 78, 157, 813, 886, 906, 977
DpnII GATC 6 cut(s) 76, 155, 811, 884, 904, 975
EaeI YGGCCR 2 cut(s) 177, 451
Eco130I CCWWGG 1 cut(s) 820
Eco47I GGWCC 1 cut(s) 161
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 315, 492, 951
EcoT14I CCWWGG 1 cut(s) 820
ErhI CCWWGG 1 cut(s) 820
FaeI CATG 4 cut(s) 463, 695, 824, 986
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FatI CATG 4 cut(s) 459, 691, 820, 982
FauI CCCGC 1 cut(s) 713
FbaI TGATCA 2 cut(s) 76, 904
FblI GTMKAC 2 cut(s) 63, 826
Fnu4HI GCNGC 3 cut(s) 110, 508, 592
FokI GGATG 1 cut(s) 560
Fsp4HI GCNGC 3 cut(s) 110, 508, 592
FspBI CTAG 1 cut(s) 636
GluI GCNGC 3 cut(s) 110, 508, 592
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 3 cut(s) 179, 453, 480
HapII CCGG 2 cut(s) 153, 159
Hin1II CATG 4 cut(s) 463, 695, 824, 986
HinfI GANTC 3 cut(s) 20, 82, 992
HpaII CCGG 2 cut(s) 153, 159
HphI GGTGA 4 cut(s) 142, 275, 788, 914
Hpy166II GTNNAC 3 cut(s) 64, 266, 827
Hpy188I TCNGA 7 cut(s) 81, 87, 135, 191, 324, 523, 889
Hpy188III TCNNGA 5 cut(s) 159, 704, 960, 989, 999
Hpy8I GTNNAC 3 cut(s) 64, 266, 827
Hpy99I CGWCG 1 cut(s) 884
HpyAV CCTTC 3 cut(s) 183, 319, 637
HpyCH4III ACNGT 1 cut(s) 831
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 3 cut(s) 591, 682, 863
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 55, 109, 591
HpyF3I CTNAG 4 cut(s) 84, 269, 503, 772
HpySE526I ACGT 1 cut(s) 433
Hsp92II CATG 4 cut(s) 463, 695, 824, 986
Kpn2I TCCGGA 1 cut(s) 158
Ksp22I TGATCA 2 cut(s) 76, 904
Kzo9I GATC 6 cut(s) 76, 155, 811, 884, 904, 975
LmnI GCTCC 3 cut(s) 94, 495, 736
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 2 cut(s) 45, 691
MaeI CTAG 1 cut(s) 636
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 1 cut(s) 628
MalI GATC 6 cut(s) 78, 157, 813, 886, 906, 977
MboI GATC 6 cut(s) 76, 155, 811, 884, 904, 975
MboII GAAGA 6 cut(s) 410, 413, 853, 944, 956, 987
MfeI CAATTG 2 cut(s) 120, 276
MflI RGATCY 2 cut(s) 155, 884
MhlI GDGCHC 1 cut(s) 105
MlsI TGGCCA 1 cut(s) 453
MluCI AATT 8 cut(s) 120, 146, 203, 276, 291, 924, 935, 963
MluNI TGGCCA 1 cut(s) 453
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 268, 525
Mox20I TGGCCA 1 cut(s) 453
MroI TCCGGA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 760
MscI TGGCCA 1 cut(s) 453
MseI TTAA 6 cut(s) 6, 27, 206, 360, 570, 696
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 594
MspI CCGG 2 cut(s) 153, 159
MspR9I CCNGG 3 cut(s) 317, 494, 953
MunI CAATTG 2 cut(s) 120, 276
MvaI CCWGG 3 cut(s) 317, 494, 953
MwoI GCNNNNNNNGC 4 cut(s) 38, 55, 109, 591
NcoI CCATGG 1 cut(s) 820
NdeII GATC 6 cut(s) 76, 155, 811, 884, 904, 975
NlaIII CATG 4 cut(s) 463, 695, 824, 986
NlaIV GGNNCC 2 cut(s) 157, 244
NmuCI GTSAC 1 cut(s) 628
PdmI GAANNNNTTC 1 cut(s) 760
PfeI GAWTC 2 cut(s) 20, 992
PflMI CCANNNNNTGG 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 3 cut(s) 111, 509, 593
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 1 cut(s) 434
Psp6I CCWGG 3 cut(s) 315, 492, 951
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 3 cut(s) 315, 492, 951
PspN4I GGNNCC 2 cut(s) 157, 244
PspPI GGNCC 1 cut(s) 161
PsuI RGATCY 2 cut(s) 155, 884
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 6 cut(s) 6, 27, 206, 360, 570, 696
SatI GCNGC 3 cut(s) 110, 508, 592
Sau3AI GATC 6 cut(s) 76, 155, 811, 884, 904, 975
Sau96I GGNCC 1 cut(s) 161
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 317, 494, 953
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 2 cut(s) 45, 691
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 98, 806
SmoI CTYRAG 2 cut(s) 98, 806
Sse9I AATT 8 cut(s) 120, 146, 203, 276, 291, 924, 935, 963
SsiI CCGC 4 cut(s) 110, 508, 594, 720
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 3 cut(s) 315, 492, 951
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 1 cut(s) 831
TaiI ACGT 1 cut(s) 436
TaqII GACCGA 1 cut(s) 158
TasI AATT 8 cut(s) 120, 146, 203, 276, 291, 924, 935, 963
TauI GCSGC 2 cut(s) 112, 510
TfiI GAWTC 2 cut(s) 20, 992
Tru1I TTAA 6 cut(s) 6, 27, 206, 360, 570, 696
Tru9I TTAA 6 cut(s) 6, 27, 206, 360, 570, 696
TscAI CASTG 3 cut(s) 262, 821, 870
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 1 cut(s) 591
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 2 cut(s) 680, 795
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 3 cut(s) 262, 821, 870
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 161
XapI RAATTY 2 cut(s) 146, 924
XmiI GTMKAC 2 cut(s) 63, 826
XmnI GAANNNNTTC 1 cut(s) 760
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.