Rroxscaffold_2G00132310

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000202
Physical Location & Seq
Forward (+)
69344420 .. 69347388
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132310.1

Sequence Viewer

Length: 1023 bp
ATGGAACTAACTGATATGTACTCACGCTTCAACTTTCGAAGATTTACTTTCTTCTCAAAGATAAAGGGTTGGCTGGTCAAAATAAAACAGGAGCTTGAGCACTTGCCGGAACAACAAAATGGCAATCCTCGGGAAAAGACCGAATTCACTGGATCCGGACCTAAATTTTCAGCCAAAGTTTTCGGATTTGATGAGCAATTAAAGGAGATTAAAGCAAAGGGTTCCAGTTCTAGCAGTGCTGGTAAATTGACGGCAATTGGGGTAGTTGGCATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTGGAGAACGAGGAAGTGAAGAAGTTTAATCATATACTGTGGCTACCCTTTTCGGGTATGAGTGAAGGAGAACAACAATATAGTAGGTTTAGCTTTGAAACACGTATCTTTGATGGTAAGGGCCACACCATAGAGGAGAGGGTTGTTGGCCTTGATAAGCTCCTGGAAAGGCTAAGCCGCGCGCGCCTGCTGTTATCAGGTAAATGCTATTTGATTGTGTTGGATGATGTGTGTCATCGGCACATTAACATTATGGAACGCTTATGCGGCGGATTGCCCAAGCATAATGGTAGTGCTATAATTGTCACTACTAGATTGAAGGAAGTAGCACTAAAGCTGGGGAAACAACATGAGTTGAAATTGGTTCATGTTAAGCCTCTGGACGGAAAGATTTGTGGGAGTATATTTCTGGAGCACTTGTTATCACGTGATGAAGTTAAGACAAAGATTGGGGAAAACGTATCAAGAGAAGAGATATTATCAAAGATGGAGGAAATCTCCGTATCAAGAGAAAATATTGAGGAAATCAAGAATCGGTGCCACGGTCTACCGTTGGTAGCGAAGACAATCGCAAACACAATTCCTGTGGAGAAGTCAAAGACTGTAATTCAACTTATCTCAGGAGGCAGTCTTGACTTATCCGCTACCAACCCTAAGGATGGCAAAGGCGGCGATGGGGATGATCGGAGCAGGCTAAGCTCCGAGCCGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

340

Amino Acids

37.93

Weight (kDa)

9.11

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 79 - 220 7.3e-17 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 846
AccI GTMKAC 1 cut(s) 856
AccII CGCG 3 cut(s) 489, 491, 493
AccIII TCCGGA 1 cut(s) 155
AciI CCGC 5 cut(s) 487, 576, 579, 951, 978
AclWI GGATC 2 cut(s) 147, 160
AcsI RAATTY 2 cut(s) 143, 164
AcvI CACGTG 1 cut(s) 737
AfaI GTAC 1 cut(s) 20
AfiI CCNNNNNNNGG 3 cut(s) 362, 692, 968
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 5 cut(s) 31, 407, 628, 667, 920
AjnI CCWGG 2 cut(s) 294, 471
AluBI AGCT 5 cut(s) 94, 402, 469, 646, 1008
AluI AGCT 5 cut(s) 94, 402, 469, 646, 1008
Alw21I GWGCWC 2 cut(s) 102, 726
AlwI GGATC 2 cut(s) 147, 160
Ama87I CYCGRG 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 155
AoxI GGCC 2 cut(s) 430, 457
ApoI RAATTY 2 cut(s) 143, 164
AspLEI GCGC 3 cut(s) 491, 493, 495
AspS9I GGNCC 2 cut(s) 158, 430
AsuII TTCGAA 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 1 cut(s) 158
AxyI CCTNAGG 1 cut(s) 963
BamHI GGATCC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 846
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BbrPI CACGTG 1 cut(s) 737
BbsI GAAGAC 1 cut(s) 878
Bbv12I GWGCWC 2 cut(s) 102, 726
BccI CCATC 4 cut(s) 416, 790, 962, 977
BceAI ACGGC 1 cut(s) 267
BciT130I CCWGG 2 cut(s) 296, 473
BfaI CTAG 2 cut(s) 231, 621
BisI GCNGC 3 cut(s) 487, 577, 979
BlpI GCTNAGC 2 cut(s) 482, 1004
BlsI GCNGC 3 cut(s) 488, 578, 980
Bme1390I CCNGG 2 cut(s) 296, 473
Bme18I GGWCC 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 158, 430
BmiI GGNNCC 3 cut(s) 154, 223, 848
BmrFI CCNGG 2 cut(s) 296, 473
BpiI GAAGAC 1 cut(s) 878
BplI GAGNNNNNCTC 2 cut(s) 791, 823
BpmI CTGGAG 2 cut(s) 297, 740
Bpu1102I GCTNAGC 2 cut(s) 482, 1004
Bpu14I TTCGAA 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 116
BsaAI YACGTR 2 cut(s) 413, 737
BsaJI CCNNGG 3 cut(s) 128, 294, 850
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 3 cut(s) 362, 692, 968
Bse118I RCCGGY 1 cut(s) 1015
Bse1I ACTGG 2 cut(s) 154, 225
Bse21I CCTNAGG 1 cut(s) 963
Bse3DI GCAATG 1 cut(s) 270
BseAI TCCGGA 1 cut(s) 155
BseBI CCWGG 2 cut(s) 296, 473
BseDI CCNNGG 3 cut(s) 128, 294, 850
BseGI GGATG 3 cut(s) 538, 973, 994
BseLI CCNNNNNNNGG 3 cut(s) 362, 692, 968
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 942
BseNI ACTGG 2 cut(s) 154, 225
BsePI GCGCGC 2 cut(s) 489, 491
BseRI GAGGAG 1 cut(s) 458
BseYI CCCAGC 1 cut(s) 646
Bsh1236I CGCG 3 cut(s) 489, 491, 493
BshFI GGCC 2 cut(s) 432, 459
BshNI GGYRCC 1 cut(s) 846
BsiHKAI GWGCWC 2 cut(s) 102, 726
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 3 cut(s) 107, 156, 1016
BslI CCNNNNNNNGG 3 cut(s) 362, 692, 968
BsnI GGCC 2 cut(s) 432, 459
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 102, 726
Bsp13I TCCGGA 1 cut(s) 155
Bsp143I GATC 2 cut(s) 152, 991
Bsp1720I GCTNAGC 2 cut(s) 482, 1004
BspACI CCGC 5 cut(s) 487, 576, 579, 951, 978
BspANI GGCC 2 cut(s) 432, 459
BspCNI CTCAG 1 cut(s) 941
BspEI TCCGGA 1 cut(s) 155
BspFNI CGCG 3 cut(s) 489, 491, 493
BspLI GGNNCC 3 cut(s) 154, 223, 848
BspPI GGATC 2 cut(s) 147, 160
BspT104I TTCGAA 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 846
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 1015
BsrI ACTGG 2 cut(s) 154, 225
BssAI RCCGGY 1 cut(s) 1015
BssECI CCNNGG 3 cut(s) 128, 294, 850
BssHII GCGCGC 2 cut(s) 489, 491
BssMI GATC 2 cut(s) 152, 991
Bst2UI CCWGG 2 cut(s) 296, 473
Bst4CI ACNGT 4 cut(s) 348, 854, 861, 913
Bst6I CTCTTC 1 cut(s) 774
BstBAI YACGTR 2 cut(s) 413, 737
BstBI TTCGAA 1 cut(s) 37
BstC8I GCNNGC 5 cut(s) 491, 493, 497, 1001, 1017
BstDEI CTNAG 4 cut(s) 482, 928, 963, 1004
BstDSI CCRYGG 1 cut(s) 850
BstF5I GGATG 3 cut(s) 538, 973, 994
BstFNI CGCG 3 cut(s) 489, 491, 493
BstHHI GCGC 3 cut(s) 491, 493, 495
BstKTI GATC 2 cut(s) 155, 994
BstMBI GATC 2 cut(s) 152, 991
BstMWI GCNNNNNNNGC 4 cut(s) 492, 576, 978, 1005
BstNI CCWGG 2 cut(s) 296, 473
BstSCI CCNGG 2 cut(s) 294, 471
BstUI CGCG 3 cut(s) 489, 491, 493
BstV2I GAAGAC 1 cut(s) 878
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 963
BsuRI GGCC 2 cut(s) 432, 459
BtgI CCRYGG 1 cut(s) 850
BtgZI GCGATG 1 cut(s) 996
BtsCI GGATG 3 cut(s) 538, 973, 994
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 147, 241
Cac8I GCNNGC 5 cut(s) 491, 493, 497, 1001, 1017
CfoI GCGC 3 cut(s) 491, 493, 495
Cfr10I RCCGGY 1 cut(s) 1015
Cfr13I GGNCC 2 cut(s) 158, 430
Csp6I GTAC 1 cut(s) 19
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviAII CATG 2 cut(s) 659, 677
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 4 cut(s) 482, 928, 963, 1004
DpnI GATC 2 cut(s) 154, 993
DpnII GATC 2 cut(s) 152, 991
Eam1104I CTCTTC 1 cut(s) 774
EarI CTCTTC 1 cut(s) 774
EciI GGCGGA 1 cut(s) 594
Eco47I GGWCC 1 cut(s) 158
Eco72I CACGTG 1 cut(s) 737
Eco81I CCTNAGG 1 cut(s) 963
Eco88I CYCGRG 1 cut(s) 129
EcoRI GAATTC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 294, 471
FaeI CATG 2 cut(s) 662, 680
FalI AAGNNNNNCTT 2 cut(s) 31, 63
FatI CATG 2 cut(s) 658, 676
FblI GTMKAC 1 cut(s) 856
Fnu4HI GCNGC 3 cut(s) 487, 577, 979
FokI GGATG 3 cut(s) 545, 980, 1001
Fsp4HI GCNGC 3 cut(s) 487, 577, 979
FspBI CTAG 2 cut(s) 231, 621
GlaI GCGC 3 cut(s) 490, 492, 494
GluI GCNGC 3 cut(s) 487, 577, 979
GsaI CCCAGC 1 cut(s) 650
GsuI CTGGAG 2 cut(s) 297, 740
HaeIII GGCC 2 cut(s) 432, 459
HapII CCGG 3 cut(s) 107, 156, 1016
HhaI GCGC 3 cut(s) 491, 493, 495
Hin1II CATG 2 cut(s) 662, 680
Hin6I GCGC 3 cut(s) 489, 491, 493
HinP1I GCGC 3 cut(s) 489, 491, 493
HinfI GANTC 1 cut(s) 841
HpaII CCGG 3 cut(s) 107, 156, 1016
Hpy166II GTNNAC 1 cut(s) 857
Hpy188I TCNGA 4 cut(s) 185, 303, 996, 1012
Hpy188III TCNNGA 9 cut(s) 131, 156, 689, 719, 774, 816, 838, 930, 941
Hpy8I GTNNAC 1 cut(s) 857
HpyAV CCTTC 3 cut(s) 298, 368, 622
HpyCH4III ACNGT 4 cut(s) 348, 854, 861, 913
HpyCH4IV ACGT 3 cut(s) 412, 736, 768
HpyF10VI GCNNNNNNNGC 4 cut(s) 492, 576, 978, 1005
HpyF3I CTNAG 4 cut(s) 482, 928, 963, 1004
HpySE526I ACGT 3 cut(s) 412, 736, 768
Hsp92II CATG 2 cut(s) 662, 680
HspAI GCGC 3 cut(s) 489, 491, 493
Kpn2I TCCGGA 1 cut(s) 155
KroI GCCGGC 1 cut(s) 1015
KroNI GCCGGC 1 cut(s) 1017
Kzo9I GATC 2 cut(s) 152, 991
LmnI GCTCC 5 cut(s) 91, 474, 721, 996, 1013
MaeI CTAG 2 cut(s) 231, 621
MaeII ACGT 3 cut(s) 412, 736, 768
MaeIII GTNAC 1 cut(s) 613
MalI GATC 2 cut(s) 154, 993
MauBI CGCGCGCG 1 cut(s) 489
MboI GATC 2 cut(s) 152, 991
MboII GAAGA 5 cut(s) 43, 51, 340, 791, 883
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 2 cut(s) 102, 726
MluCI AATT 9 cut(s) 143, 164, 197, 245, 255, 609, 668, 888, 915
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 8 cut(s) 138, 313, 436, 441, 696, 793, 823, 926
MroI TCCGGA 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 1015
MseI TTAA 7 cut(s) 200, 210, 336, 555, 681, 747, 1021
MspI CCGG 3 cut(s) 107, 156, 1016
MspR9I CCNGG 2 cut(s) 296, 473
MunI CAATTG 1 cut(s) 255
MvaI CCWGG 2 cut(s) 296, 473
MvnI CGCG 3 cut(s) 489, 491, 493
MwoI GCNNNNNNNGC 4 cut(s) 492, 576, 978, 1005
NaeI GCCGGC 1 cut(s) 1017
NdeII GATC 2 cut(s) 152, 991
NgoMIV GCCGGC 1 cut(s) 1015
NlaIII CATG 2 cut(s) 662, 680
NlaIV GGNNCC 3 cut(s) 154, 223, 848
NmuCI GTSAC 1 cut(s) 613
NspV TTCGAA 1 cut(s) 37
PauI GCGCGC 2 cut(s) 489, 491
PdiI GCCGGC 1 cut(s) 1017
PfeI GAWTC 1 cut(s) 841
PfoI TCCNGGA 1 cut(s) 471
PkrI GCNGC 3 cut(s) 488, 578, 980
PmaCI CACGTG 1 cut(s) 737
PmlI CACGTG 1 cut(s) 737
Ppu21I YACGTR 2 cut(s) 413, 737
Psp6I CCWGG 2 cut(s) 294, 471
PspCI CACGTG 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 646
PspGI CCWGG 2 cut(s) 294, 471
PspN4I GGNNCC 3 cut(s) 154, 223, 848
PspPI GGNCC 2 cut(s) 158, 430
PsuI RGATCY 1 cut(s) 152
PteI GCGCGC 2 cut(s) 489, 491
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
SaqAI TTAA 7 cut(s) 200, 210, 336, 555, 681, 747, 1021
SatI GCNGC 3 cut(s) 487, 577, 979
Sau3AI GATC 2 cut(s) 152, 991
Sau96I GGNCC 2 cut(s) 158, 430
ScrFI CCNGG 2 cut(s) 296, 473
SduI GDGCHC 2 cut(s) 102, 726
SfuI TTCGAA 1 cut(s) 37
SinI GGWCC 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 9 cut(s) 143, 164, 197, 245, 255, 609, 668, 888, 915
SsiI CCGC 5 cut(s) 487, 576, 579, 951, 978
SspI AATATT 1 cut(s) 826
SspMI CTAG 2 cut(s) 231, 621
StyD4I CCNGG 2 cut(s) 294, 471
TaaI ACNGT 4 cut(s) 348, 854, 861, 913
TaiI ACGT 3 cut(s) 415, 739, 771
TaqI TCGA 1 cut(s) 37
TaqII GACCGA 1 cut(s) 155
TasI AATT 9 cut(s) 143, 164, 197, 245, 255, 609, 668, 888, 915
TatI WGTACW 1 cut(s) 18
TauI GCSGC 3 cut(s) 489, 579, 981
TfiI GAWTC 1 cut(s) 841
Tru1I TTAA 7 cut(s) 200, 210, 336, 555, 681, 747, 1021
Tru9I TTAA 7 cut(s) 200, 210, 336, 555, 681, 747, 1021
TscAI CASTG 2 cut(s) 154, 241
TseFI GTSAC 1 cut(s) 613
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 2 cut(s) 665, 756
TspGWI ACGGA 2 cut(s) 708, 799
TspRI CASTG 2 cut(s) 154, 241
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 2 cut(s) 143, 164
XmiI GTMKAC 1 cut(s) 856
XspI CTAG 2 cut(s) 231, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.