ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 1141 |
| AccII | CGCG | 1 cut(s) | 125 |
| AccIII | TCCGGA | 1 cut(s) | 419 |
| AciI | CCGC | 3 cut(s) | 371, 769, 855 |
| AclWI | GGATC | 2 cut(s) | 411, 424 |
| AcoI | YGGCCR | 2 cut(s) | 339, 712 |
| AcsI | RAATTY | 3 cut(s) | 20, 131, 407 |
| AcvI | CACGTG | 1 cut(s) | 1013 |
| AfaI | GTAC | 2 cut(s) | 284, 718 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 188, 644, 968, 1136, 1184 |
| AflIII | ACRYGT | 1 cut(s) | 692 |
| AgsI | TTSAA | 5 cut(s) | 103, 136, 295, 689, 904 |
| AjnI | CCWGG | 2 cut(s) | 573, 753 |
| AleI | CACNNNNGTG | 1 cut(s) | 259 |
| AluBI | AGCT | 6 cut(s) | 58, 358, 684, 751, 914, 922 |
| AluI | AGCT | 6 cut(s) | 58, 358, 684, 751, 914, 922 |
| Alw21I | GWGCWC | 3 cut(s) | 213, 1002, 1071 |
| Alw26I | GTCTC | 1 cut(s) | 51 |
| AlwI | GGATC | 2 cut(s) | 411, 424 |
| Ama87I | CYCGRG | 1 cut(s) | 393 |
| Aor13HI | TCCGGA | 1 cut(s) | 419 |
| AoxI | GGCC | 3 cut(s) | 339, 712, 739 |
| ApeKI | GCWGC | 2 cut(s) | 634, 852 |
| ApoI | RAATTY | 3 cut(s) | 20, 131, 407 |
| AseI | ATTAAT | 1 cut(s) | 474 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 35 |
| AspS9I | GGNCC | 1 cut(s) | 422 |
| AsuII | TTCGAA | 1 cut(s) | 301 |
| AvaI | CYCGRG | 1 cut(s) | 393 |
| AvaII | GGWCC | 1 cut(s) | 422 |
| BalI | TGGCCA | 1 cut(s) | 341 |
| BamHI | GGATCC | 1 cut(s) | 416 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 275, 307 |
| BbrPI | CACGTG | 1 cut(s) | 1013 |
| BbsI | GAAGAC | 3 cut(s) | 91, 241, 1163 |
| Bbv12I | GWGCWC | 3 cut(s) | 213, 1002, 1071 |
| BbvI | GCAGC | 2 cut(s) | 621, 864 |
| BccI | CCATC | 3 cut(s) | 98, 698, 1078 |
| BceAI | ACGGC | 2 cut(s) | 546, 699 |
| BciT130I | CCWGG | 2 cut(s) | 575, 755 |
| BcoDI | GTCTC | 1 cut(s) | 51 |
| BfaI | CTAG | 4 cut(s) | 50, 59, 510, 897 |
| BglII | AGATCT | 1 cut(s) | 118 |
| BisI | GCNGC | 4 cut(s) | 371, 635, 769, 853 |
| BlpI | GCTNAGC | 1 cut(s) | 764 |
| BlsI | GCNGC | 4 cut(s) | 372, 636, 770, 854 |
| Bme1390I | CCNGG | 2 cut(s) | 575, 755 |
| Bme18I | GGWCC | 1 cut(s) | 422 |
| BmeT110I | CYCGRG | 1 cut(s) | 393 |
| BmgT120I | GGNCC | 1 cut(s) | 422 |
| BmiI | GGNNCC | 1 cut(s) | 418 |
| BmrFI | CCNGG | 2 cut(s) | 575, 755 |
| BmsI | GCATC | 1 cut(s) | 217 |
| BpiI | GAAGAC | 3 cut(s) | 91, 241, 1163 |
| BpmI | CTGGAG | 2 cut(s) | 576, 1016 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 764 |
| Bpu14I | TTCGAA | 1 cut(s) | 301 |
| BpuEI | CTTGAG | 1 cut(s) | 380 |
| BsaAI | YACGTR | 2 cut(s) | 695, 1013 |
| BsaJI | CCNNGG | 3 cut(s) | 392, 573, 1135 |
| BsaWI | WCCGGW | 1 cut(s) | 419 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1058, 1088 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 188, 644, 968, 1136, 1184 |
| Bse118I | RCCGGY | 1 cut(s) | 188 |
| Bse1I | ACTGG | 2 cut(s) | 418, 504 |
| Bse3DI | GCAATG | 1 cut(s) | 549 |
| BseAI | TCCGGA | 1 cut(s) | 419 |
| BseBI | CCWGG | 2 cut(s) | 575, 755 |
| BseDI | CCNNGG | 3 cut(s) | 392, 573, 1135 |
| BseGI | GGATG | 2 cut(s) | 16, 814 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 188, 644, 968, 1136, 1184 |
| BseMI | GCAATG | 1 cut(s) | 549 |
| BseMII | CTCAG | 2 cut(s) | 207, 228 |
| BseNI | ACTGG | 2 cut(s) | 418, 504 |
| BseRI | GAGGAG | 2 cut(s) | 740, 1079 |
| BseXI | GCAGC | 2 cut(s) | 621, 864 |
| BseYI | CCCAGC | 1 cut(s) | 922 |
| Bsh1236I | CGCG | 1 cut(s) | 125 |
| BshFI | GGCC | 3 cut(s) | 341, 714, 741 |
| BsiHKAI | GWGCWC | 3 cut(s) | 213, 1002, 1071 |
| BsiHKCI | CYCGRG | 1 cut(s) | 393 |
| BsiSI | CCGG | 2 cut(s) | 189, 420 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 188, 644, 968, 1136, 1184 |
| BsmAI | GTCTC | 1 cut(s) | 51 |
| BsnI | GGCC | 3 cut(s) | 341, 714, 741 |
| BsoBI | CYCGRG | 1 cut(s) | 393 |
| Bsp119I | TTCGAA | 1 cut(s) | 301 |
| Bsp1286I | GDGCHC | 3 cut(s) | 213, 1002, 1071 |
| Bsp13I | TCCGGA | 1 cut(s) | 419 |
| Bsp143I | GATC | 2 cut(s) | 118, 416 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 764 |
| BspACI | CCGC | 3 cut(s) | 371, 769, 855 |
| BspANI | GGCC | 3 cut(s) | 341, 714, 741 |
| BspCNI | CTCAG | 2 cut(s) | 206, 229 |
| BspEI | TCCGGA | 1 cut(s) | 419 |
| BspFNI | CGCG | 1 cut(s) | 125 |
| BspLI | GGNNCC | 1 cut(s) | 418 |
| BspPI | GGATC | 2 cut(s) | 411, 424 |
| BspT104I | TTCGAA | 1 cut(s) | 301 |
| BsrDI | GCAATG | 1 cut(s) | 549 |
| BsrFI | RCCGGY | 1 cut(s) | 188 |
| BsrI | ACTGG | 2 cut(s) | 418, 504 |
| BssAI | RCCGGY | 1 cut(s) | 188 |
| BssECI | CCNNGG | 3 cut(s) | 392, 573, 1135 |
| BssMI | GATC | 2 cut(s) | 118, 416 |
| Bst2UI | CCWGG | 2 cut(s) | 575, 755 |
| Bst4CI | ACNGT | 3 cut(s) | 630, 1139, 1146 |
| Bst6I | CTCTTC | 1 cut(s) | 1050 |
| BstBAI | YACGTR | 2 cut(s) | 695, 1013 |
| BstBI | TTCGAA | 1 cut(s) | 301 |
| BstC8I | GCNNGC | 2 cut(s) | 339, 773 |
| BstDEI | CTNAG | 3 cut(s) | 193, 237, 764 |
| BstDSI | CCRYGG | 1 cut(s) | 1135 |
| BstF5I | GGATG | 2 cut(s) | 16, 814 |
| BstFNI | CGCG | 1 cut(s) | 125 |
| BstKTI | GATC | 2 cut(s) | 121, 419 |
| BstMAI | GTCTC | 1 cut(s) | 51 |
| BstMBI | GATC | 2 cut(s) | 118, 416 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 852 |
| BstNI | CCWGG | 2 cut(s) | 575, 755 |
| BstSCI | CCNGG | 2 cut(s) | 573, 753 |
| BstUI | CGCG | 1 cut(s) | 125 |
| BstV1I | GCAGC | 2 cut(s) | 621, 864 |
| BstV2I | GAAGAC | 3 cut(s) | 91, 241, 1163 |
| BstX2I | RGATCY | 2 cut(s) | 118, 416 |
| BstYI | RGATCY | 2 cut(s) | 118, 416 |
| BsuRI | GGCC | 3 cut(s) | 341, 714, 741 |
| BtgI | CCRYGG | 1 cut(s) | 1135 |
| BtsCI | GGATG | 2 cut(s) | 16, 814 |
| BtsI | GCAGTG | 1 cut(s) | 520 |
| BtsIMutI | CAGTG | 2 cut(s) | 411, 520 |
| Cac8I | GCNNGC | 2 cut(s) | 339, 773 |
| Cfr10I | RCCGGY | 1 cut(s) | 188 |
| Cfr13I | GGNCC | 1 cut(s) | 422 |
| CseI | GACGC | 1 cut(s) | 114 |
| Csp6I | GTAC | 2 cut(s) | 283, 717 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1161, 1196 |
| CviAII | CATG | 3 cut(s) | 257, 935, 953 |
| CviQI | GTAC | 2 cut(s) | 283, 717 |
| DdeI | CTNAG | 3 cut(s) | 193, 237, 764 |
| DpnI | GATC | 2 cut(s) | 120, 418 |
| DpnII | GATC | 2 cut(s) | 118, 416 |
| EaeI | YGGCCR | 2 cut(s) | 339, 712 |
| Eam1104I | CTCTTC | 1 cut(s) | 1050 |
| EarI | CTCTTC | 1 cut(s) | 1050 |
| Eco47I | GGWCC | 1 cut(s) | 422 |
| Eco72I | CACGTG | 1 cut(s) | 1013 |
| Eco88I | CYCGRG | 1 cut(s) | 393 |
| EcoRI | GAATTC | 3 cut(s) | 20, 131, 407 |
| EcoRII | CCWGG | 2 cut(s) | 573, 753 |
| EcoT22I | ATGCAT | 1 cut(s) | 232 |
| FaeI | CATG | 3 cut(s) | 260, 938, 956 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 295, 327 |
| FatI | CATG | 3 cut(s) | 256, 934, 952 |
| FblI | GTMKAC | 1 cut(s) | 1141 |
| Fnu4HI | GCNGC | 4 cut(s) | 371, 635, 769, 853 |
| FokI | GGATG | 2 cut(s) | 23, 821 |
| Fsp4HI | GCNGC | 4 cut(s) | 371, 635, 769, 853 |
| FspBI | CTAG | 4 cut(s) | 50, 59, 510, 897 |
| GluI | GCNGC | 4 cut(s) | 371, 635, 769, 853 |
| GsaI | CCCAGC | 1 cut(s) | 926 |
| GsuI | CTGGAG | 2 cut(s) | 576, 1016 |
| HaeIII | GGCC | 3 cut(s) | 341, 714, 741 |
| HapII | CCGG | 2 cut(s) | 189, 420 |
| HgaI | GACGC | 1 cut(s) | 114 |
| Hin1II | CATG | 3 cut(s) | 260, 938, 956 |
| HincII | GTYRAC | 1 cut(s) | 942 |
| HindII | GTYRAC | 1 cut(s) | 942 |
| HinfI | GANTC | 5 cut(s) | 62, 68, 246, 1126, 1188 |
| HpaII | CCGG | 2 cut(s) | 189, 420 |
| Hpy166II | GTNNAC | 2 cut(s) | 942, 1142 |
| Hpy188I | TCNGA | 5 cut(s) | 67, 130, 196, 449, 582 |
| Hpy8I | GTNNAC | 2 cut(s) | 942, 1142 |
| HpyAV | CCTTC | 3 cut(s) | 577, 650, 898 |
| HpyCH4III | ACNGT | 3 cut(s) | 630, 1139, 1146 |
| HpyCH4IV | ACGT | 3 cut(s) | 694, 1012, 1044 |
| HpyCH4V | TGCA | 3 cut(s) | 230, 264, 852 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 852 |
| HpyF3I | CTNAG | 3 cut(s) | 193, 237, 764 |
| HpySE526I | ACGT | 3 cut(s) | 694, 1012, 1044 |
| Hsp92II | CATG | 3 cut(s) | 260, 938, 956 |
| Kpn2I | TCCGGA | 1 cut(s) | 419 |
| Kzo9I | GATC | 2 cut(s) | 118, 416 |
| LmnI | GCTCC | 4 cut(s) | 355, 756, 997, 1066 |
| Lsp1109I | GCAGC | 2 cut(s) | 621, 864 |
| LweI | GCATC | 1 cut(s) | 217 |
| MaeI | CTAG | 4 cut(s) | 50, 59, 510, 897 |
| MaeII | ACGT | 3 cut(s) | 694, 1012, 1044 |
| MaeIII | GTNAC | 2 cut(s) | 889, 1013 |
| MalI | GATC | 2 cut(s) | 120, 418 |
| MboI | GATC | 2 cut(s) | 118, 416 |
| MboII | GAAGA | 7 cut(s) | 91, 241, 315, 622, 691, 1067, 1168 |
| MfeI | CAATTG | 1 cut(s) | 534 |
| MflI | RGATCY | 2 cut(s) | 118, 416 |
| MhlI | GDGCHC | 3 cut(s) | 213, 1002, 1071 |
| MlsI | TGGCCA | 1 cut(s) | 341 |
| MluNI | TGGCCA | 1 cut(s) | 341 |
| MlyI | GAGTC | 3 cut(s) | 71, 255, 1197 |
| MmeI | TCCRAC | 1 cut(s) | 786 |
| Mox20I | TGGCCA | 1 cut(s) | 341 |
| Mph1103I | ATGCAT | 1 cut(s) | 232 |
| MroI | TCCGGA | 1 cut(s) | 419 |
| MroXI | GAANNNNTTC | 1 cut(s) | 35 |
| MscI | TGGCCA | 1 cut(s) | 341 |
| MseI | TTAA | 6 cut(s) | 464, 474, 618, 831, 957, 1023 |
| MslI | CAYNNNNRTG | 1 cut(s) | 259 |
| Msp20I | TGGCCA | 1 cut(s) | 341 |
| MspA1I | CMGCKG | 1 cut(s) | 855 |
| MspI | CCGG | 2 cut(s) | 189, 420 |
| MspR9I | CCNGG | 2 cut(s) | 575, 755 |
| MunI | CAATTG | 1 cut(s) | 534 |
| MvaI | CCWGG | 2 cut(s) | 575, 755 |
| MvnI | CGCG | 1 cut(s) | 125 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 852 |
| NdeII | GATC | 2 cut(s) | 118, 416 |
| NlaIII | CATG | 3 cut(s) | 260, 938, 956 |
| NlaIV | GGNNCC | 1 cut(s) | 418 |
| NmeAIII | GCCGAG | 1 cut(s) | 269 |
| NmuCI | GTSAC | 2 cut(s) | 889, 1013 |
| NsiI | ATGCAT | 1 cut(s) | 232 |
| NspV | TTCGAA | 1 cut(s) | 301 |
| OliI | CACNNNNGTG | 1 cut(s) | 259 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 453 |
| PdmI | GAANNNNTTC | 1 cut(s) | 35 |
| PfeI | GAWTC | 2 cut(s) | 68, 1126 |
| PfoI | TCCNGGA | 1 cut(s) | 753 |
| PkrI | GCNGC | 4 cut(s) | 372, 636, 770, 854 |
| PleI | GAGTC | 3 cut(s) | 70, 254, 1196 |
| PmaCI | CACGTG | 1 cut(s) | 1013 |
| PmlI | CACGTG | 1 cut(s) | 1013 |
| PpsI | GAGTC | 3 cut(s) | 70, 254, 1196 |
| Ppu21I | YACGTR | 2 cut(s) | 695, 1013 |
| PshBI | ATTAAT | 1 cut(s) | 474 |
| Psp6I | CCWGG | 2 cut(s) | 573, 753 |
| PspCI | CACGTG | 1 cut(s) | 1013 |
| PspFI | CCCAGC | 1 cut(s) | 922 |
| PspGI | CCWGG | 2 cut(s) | 573, 753 |
| PspN4I | GGNNCC | 1 cut(s) | 418 |
| PspPI | GGNCC | 1 cut(s) | 422 |
| PsuI | RGATCY | 2 cut(s) | 118, 416 |
| RsaI | GTAC | 2 cut(s) | 284, 718 |
| RsaNI | GTAC | 2 cut(s) | 283, 717 |
| RseI | CAYNNNNRTG | 1 cut(s) | 259 |
| SaqAI | TTAA | 6 cut(s) | 464, 474, 618, 831, 957, 1023 |
| SatI | GCNGC | 4 cut(s) | 371, 635, 769, 853 |
| Sau3AI | GATC | 2 cut(s) | 118, 416 |
| Sau96I | GGNCC | 1 cut(s) | 422 |
| SchI | GAGTC | 3 cut(s) | 71, 255, 1197 |
| ScrFI | CCNGG | 2 cut(s) | 575, 755 |
| SduI | GDGCHC | 3 cut(s) | 213, 1002, 1071 |
| SfaNI | GCATC | 1 cut(s) | 217 |
| SfuI | TTCGAA | 1 cut(s) | 301 |
| SinI | GGWCC | 1 cut(s) | 422 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 259 |
| SmlI | CTYRAG | 1 cut(s) | 359 |
| SmoI | CTYRAG | 1 cut(s) | 359 |
| SsiI | CCGC | 3 cut(s) | 371, 769, 855 |
| SspMI | CTAG | 4 cut(s) | 50, 59, 510, 897 |
| StyD4I | CCNGG | 2 cut(s) | 573, 753 |
| TaaI | ACNGT | 3 cut(s) | 630, 1139, 1146 |
| TaiI | ACGT | 3 cut(s) | 697, 1015, 1047 |
| TaqI | TCGA | 2 cut(s) | 301, 702 |
| TaqII | GACCGA | 1 cut(s) | 419 |
| TatI | WGTACW | 1 cut(s) | 282 |
| TauI | GCSGC | 2 cut(s) | 373, 771 |
| TfiI | GAWTC | 2 cut(s) | 68, 1126 |
| Tru1I | TTAA | 6 cut(s) | 464, 474, 618, 831, 957, 1023 |
| Tru9I | TTAA | 6 cut(s) | 464, 474, 618, 831, 957, 1023 |
| TscAI | CASTG | 2 cut(s) | 418, 520 |
| TseFI | GTSAC | 2 cut(s) | 889, 1013 |
| TseI | GCWGC | 2 cut(s) | 634, 852 |
| Tsp45I | GTSAC | 2 cut(s) | 889, 1013 |
| TspDTI | ATGAA | 4 cut(s) | 304, 492, 623, 941 |
| TspGWI | ACGGA | 2 cut(s) | 984, 1119 |
| TspRI | CASTG | 2 cut(s) | 418, 520 |
| VpaK11BI | GGWCC | 1 cut(s) | 422 |
| VspI | ATTAAT | 1 cut(s) | 474 |
| XapI | RAATTY | 3 cut(s) | 20, 131, 407 |
| XmiI | GTMKAC | 1 cut(s) | 1141 |
| XmnI | GAANNNNTTC | 1 cut(s) | 35 |
| XspI | CTAG | 4 cut(s) | 50, 59, 510, 897 |
| Zsp2I | ATGCAT | 1 cut(s) | 232 |