RLG00000020616

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000202
Physical Location & Seq
Reverse (-)
66728872 .. 66735096
6225 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020616

Sequence Viewer

Length: 2544 bp
ATGACACCGGAAGTTGCTGAGTTCCTACGGCACAAGTTCTTGAAGTCCATTCGAGACGCAGAGTCTGAATTATCAGGTTTCATCTTCATTCGTTACTTCACAGCCATCAAATGTTTCGTGGAGGAGATAAAGATAAACCCAGCGATAAAAACCCCACTTGGATCCGGAGTTGATCTTCGCCTCAAGGCTATGTCTTTGCTCTACGATCTTAACGACGCATTGGCCGAGTGCCGAGTCTTCGCAAATGAGTGTAAGCAGATCAACAAGAAGCTCTTGCACTTCAACCCTTTCATGTATTATTTCATCCGAAAGATGAAGAAGCGCCTCCACGCAATGAAATGCAAGTTTGCTGCTTTGGTTGAAGAGGACAAATTTTATTCCATTCTTGTACTCTCTGACCAGACGAGTGTAATGGCTGACCGGATGATTCTAATTGGTGATCAGAACGAGGTTGTATTTCGAGAGCCAAAAGTGATCAGAGCCGATGACCATGCAGAGGACATTGAGGGCCTGCTCTTTGGCGGTGACGGAAAAGGATTTACGGCAATTGGGATAGTTGGCATTGCTGGTGTAGGCAAGACTACTCTGGTCCACCAGGTTTTGAATAGGCAGAGAGTGAAGGATGAGTTTTCTCCTATAATTTGGTTGTGTTTGTCGGATATAGTCAAAGAAAAAGATGTGTTTACAGATAGGATCAGCATTAGGATTGTGACCTGTATTCTCCATAAGTTGGAGGATATGGGTAATGACAATGGTGCCATAGTCGAGGAAGAGGGAGATATTTCTAGCCTTGGTTTTCTCTTGGAGAGGCTCAACGAACTTTTATCGGGTAGAAGGTATTTGATTGTATTGGATGATGTGTGGCACATTAACAACTTCTATTCGGATTTAGGTAGTACATTTCAGGATCGGTTATCGCATGCATTGCCTAAGGGTAGCGGTGGTGCTGTCATTGTCACCACTAGGATACCCGAAGTTGCTCAATACATGGTTGGCACAAGGAATATGATTCTTGTTAAGCCTTTGGATAGAGAAAGCTGCTGGCGTATATTTTTGGAGACTATCAAGGATAACAATGAAGTTTTACACATGTCAACTCATGAAACTTTGGAAAAGATTAAGAATGAAATTAAGGATCAATGTTACGGTCTACCACTTGCTGCTAAGGAACTAGCAGGAATCATTCCCAAACGGATTCGTGACATTGAGTCCAACCGCTTCTTGAAGGAGAGCTACATACCTGATGAATTGCTTCAACCTGACTTGGATGTTGAACCTGCTGTCCATATACCAGAATTCCCAGTGCTAGTGTTCATTGATATGAAAAGTAAACAGCTTGGAGAAAAACTACTTGACAAATTTCGCTACCATCTTAATAGAAAGCAGGTTTTTGCTGTTTTTGAAGAGAATTCTGATTGCAAGCAAAAGGTAAAGGCCGATAATCCTGAGAAGGTGCTAAAGGATATTTACAGTACTTTAGAAAAGCTTAAGCGTAAAGGATATGCTTTTGCTGATGAAATTCAAAAGACGATGACGATTATAATTGTTGGTCGGGATTCTGTGGCTAACTGGATATTGGGAGTTATCTGTGATCTAAAATTGCCAGAGTTACCCTTCATTGCTCCGATACCGTTGGAAGCTAGTTCTCTGATAAGTGGTAGTATCTCAATGTCATTTGGATGGAAGTGCATTTCTCTTGATAATCGAACACTGATATTCTCCCTGGAAGATATACAATATGCAAAGAAAATGAAAACTGACAGCTGGCATGTTCGCATTAGGATGAAAGCCACTCAAGAAATACCACATTCTTTGCATCTATTTCATCCTGTTCATGAAGGGAATATTCATAATGCGGATCCGCTCACATTCTTGGGAGGATTTTGGAACTACTTCAGCCTGGAGTATGTTGCTCCCCGAGGATGTGGAAATCACTGCTTTCAACCTACTCAAAGGACCTTATCATCAGTTGCATGTATAAAGGTTATGAAACAACTTGGTCAATGGGATATACTTCATATTTCTAGCAGCATCAAGTCAATTCTTTGCCTTAACTTGCCTAGACCTCAAATTATTAAGAAAGATCGGAAAGAGAGAGACCTGATTCCGTCTGTCATTGATGATGGACATCTTGAAATTATTGGTTACAGAGATGTTTTCTTTCCTCTTAGTGAAGGGTCGATTCACCGTGACCAGGTGCGGGGACTTCGTTTCGAGTTTATTGATGGTGAAGAAGGGGTTGTGAAGATGACCATTGATGGAGCATCATGGGAAAATATCCCCCTTGATGGTACTGGTATCACTATGATTGAAATCACTCATCACTGTCAAGTCAACATTCTTGCCAATTGGTTTATCGATTCCCCACTGAAAAGTATACACGATTCATCACCACCTGATGCTTCCGAAAATGATGATGAGAATGATCCTAAAGATTACTATGAACGACATAAGAAGTTTGGTGCAGCAAGCACTTTCAAACTTGCCTGTGCTGATGAAGGACGTAACACCTTTGGTGAAGCAAGCACTTCCAACGTCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

848

Amino Acids

96.38

Weight (kDa)

6.04

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 356 8.8e-26 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000202)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28130
fragaria_vesca FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230 FvH4_6g29230
malus_domestica MD17G1216600.v1.1
prunus_persica Prupe.3G175700_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175800_v2.0.a1 Prupe.3G175900_v2.0.a1 Prupe.3G176000_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.3G176200_v2.0.a1 Prupe.8G030500_v2.0.a1 Prupe.8G030500_v2.0.a1
pyrus_communis pycom17g22110
rosa_chinensis RchiOBHm_Chr1g0372981 RchiOBHm_Chr2g0112801 RchiOBHm_Chr2g0151871 RchiOBHm_Chr2g0151881 RchiOBHm_Chr2g0151891 RchiOBHm_Chr2g0151901 RchiOBHm_Chr2g0151911 RchiOBHm_Chr2g0151921 RchiOBHm_Chr2g0152191 RchiOBHm_Chr2g0152201 RchiOBHm_Chr2g0152211 RchiOBHm_Chr7g0229501 RchiOBHm_Chr7g0229661 RchiOBHm_Chr7g0229861 RchiOBHm_Chr7g0229991 RchiOBHm_Chr7g0230141
rosa_laevigata RLG00000018011 RLG00000018012 RLG00000020585 RLG00000020586 RLG00000020587 RLG00000020616
rosa_multiflora Rmu_co8278997.1_g000001 Rmu_sc0000693.1_g000084 Rmu_sc0000851.1_g000003 Rmu_sc0000851.1_g000022 Rmu_sc0001552.1_g000055 Rmu_sc0001621.1_g000002 Rmu_sc0001708.1_g000015 Rmu_sc0001708.1_g000016 Rmu_sc0001836.1_g000045 Rmu_sc0002351.1_g000009 Rmu_sc0002351.1_g000011 Rmu_sc0002351.1_g000063 Rmu_sc0002666.1_g000014 Rmu_sc0003043.1_g000011 Rmu_sc0004929.1_g000004 Rmu_sc0005294.1_g000007 Rmu_sc0005294.1_g000016 Rmu_sc0005791.1_g000002 Rmu_sc0021251.1_g000001 Rmu_sc0023925.1_g000004 Rmu_sc0034442.1_g000001
rosa_roxburghii Rroxscaffold_2G00095850 Rroxscaffold_2G00096120 Rroxscaffold_2G00131130 Rroxscaffold_2G00132310 Rroxscaffold_3G00230340
rosa_rugosa Rorug02G0174300 Rorug02G0174400 Rorug02G0182700 Rorug02G0429300 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429400 Rorug02G0429500 Rorug02G0431300 Rorug02G0431300 Rorug07G0258400 Rorug07G0258400 Rorug07G0258600 Rorug07G0259000 Rorug07G0259400
rosa_samantha Rh1BG314200 Rh1CG329100 Rh1DG131500 Rh1DG345900 Rh2AG227100 Rh2AG490100 Rh2AG490300 Rh2AG492700 Rh2AG493200 Rh2BG238900 Rh2BG251200 Rh2BG502800 Rh2BG505300 Rh2CG476400 Rh2CG476600 Rh2CG479100 Rh2CG597400 Rh2DG234700 Rh2DG244900 Rh2DG257400 Rh2DG514000 Rh2DG516500 Rh4DG198400 Rh5BG245300 Rh5BG245400 Rh5BG245500 Rh7AG408200 Rh7CG427400 Rh7CG427500 Rh7DG404000 Rh7DG404700 Rh7DG405300
rosa_wichuraiana Rw0G020250 Rw0G020260 Rw2G017530 Rw2G018370 Rw2G018410 Rw2G018560 Rw2G040190 Rw2G040310 Rw2G040490 Rw7G033910 Rw7G034050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1541
Acc36I ACCTGC 2 cut(s) 1285, 1375
AccB1I GGYRCC 1 cut(s) 755
AccB7I CCANNNNNTGG 1 cut(s) 730
AccBSI CCGCTC 1 cut(s) 1864
AccI GTMKAC 2 cut(s) 1150, 2377
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 6 cut(s) 522, 939, 1216, 1856, 1862, 2200
AclWI GGATC 8 cut(s) 156, 169, 701, 915, 1143, 1853, 1866, 2420
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 5 cut(s) 371, 1295, 1358, 1408, 1518
AcuI CTGAAG 1 cut(s) 1879
AfaI GTAC 4 cut(s) 390, 898, 1474, 2293
AfiI CCNNNNNNNGG 5 cut(s) 496, 730, 2194, 2200, 2288
AflII CTTAAG 1 cut(s) 1487
AflIII ACRYGT 1 cut(s) 1089
AhdI GACNNNNNGTC 2 cut(s) 61, 1207
AjnI CCWGG 4 cut(s) 594, 1722, 1899, 2193
AloI GAACNNNNNNTCC 2 cut(s) 1266, 1298
AluBI AGCT 7 cut(s) 271, 1038, 1233, 1336, 1486, 1640, 1764
AluI AGCT 7 cut(s) 271, 1038, 1233, 1336, 1486, 1640, 1764
Alw26I GTCTC 3 cut(s) 48, 1052, 2091
AlwI GGATC 8 cut(s) 156, 169, 701, 915, 1143, 1853, 1866, 2420
AlwNI CAGNNNCTG 1 cut(s) 65
Ama87I CYCGRG 1 cut(s) 1917
Aor13HI TCCGGA 1 cut(s) 164
AoxI GGCC 3 cut(s) 222, 508, 1434
ApeKI GCWGC 5 cut(s) 350, 1038, 1160, 2028, 2465
ApoI RAATTY 5 cut(s) 371, 1295, 1358, 1408, 1518
Asp700I GAANNNNTTC 2 cut(s) 1251, 1892
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 3 cut(s) 508, 589, 1956
AsuHPI GGTGA 7 cut(s) 449, 536, 949, 2177, 2240, 2382, 2528
AvaI CYCGRG 1 cut(s) 1917
AvaII GGWCC 2 cut(s) 589, 1956
AxyI CCTNAGG 1 cut(s) 930
BamHI GGATCC 2 cut(s) 161, 1858
BanI GGYRCC 1 cut(s) 755
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BbsI GAAGAC 1 cut(s) 229
BbvI GCAGC 5 cut(s) 337, 1025, 1147, 2040, 2477
BccI CCATC 7 cut(s) 113, 1377, 1674, 2117, 2219, 2252, 2282
BceAI ACGGC 2 cut(s) 44, 558
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 4 cut(s) 596, 1724, 1901, 2195
BciVI GTATCC 1 cut(s) 960
BclI TGATCA 2 cut(s) 439, 474
BcoDI GTCTC 3 cut(s) 48, 1052, 2091
BfaI CTAG 7 cut(s) 786, 963, 1172, 1307, 1641, 2025, 2061
BfoI RGCGCY 1 cut(s) 325
BfrI CTTAAG 1 cut(s) 1487
BfuAI ACCTGC 2 cut(s) 1285, 1375
BfuI GTATCC 1 cut(s) 960
BisI GCNGC 5 cut(s) 351, 1039, 1161, 2029, 2466
BlsI GCNGC 5 cut(s) 352, 1040, 1162, 2030, 2467
BmcAI AGTACT 1 cut(s) 1474
Bme1390I CCNGG 4 cut(s) 596, 1724, 1901, 2195
Bme18I GGWCC 2 cut(s) 589, 1956
BmeRI GACNNNNNGTC 2 cut(s) 61, 1207
BmeT110I CYCGRG 1 cut(s) 1917
BmgT120I GGNCC 3 cut(s) 508, 589, 1956
BmiI GGNNCC 3 cut(s) 163, 757, 1860
BmrFI CCNGG 4 cut(s) 596, 1724, 1901, 2195
BmrI ACTGGG 1 cut(s) 1295
BmsI GCATC 4 cut(s) 1825, 2040, 2273, 2389
BmuI ACTGGG 1 cut(s) 1295
BpiI GAAGAC 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 1922
Bpu10I CCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 2 cut(s) 167, 1779
Bsa29I ATCGAT 1 cut(s) 2358
BsaBI GATNNNNATC 2 cut(s) 2127, 2312
BsaI GGTCTC 1 cut(s) 2091
BsaJI CCNNGG 3 cut(s) 790, 1722, 1918
BsaWI WCCGGW 3 cut(s) 7, 164, 420
Bsc4I CCNNNNNNNGG 5 cut(s) 496, 730, 2194, 2200, 2288
Bse1I ACTGG 3 cut(s) 1301, 1574, 2299
Bse21I CCTNAGG 1 cut(s) 930
Bse3DI GCAATG 4 cut(s) 339, 561, 923, 1617
Bse8I GATNNNNATC 2 cut(s) 2127, 2312
BseAI TCCGGA 1 cut(s) 164
BseBI CCWGG 4 cut(s) 596, 1724, 1901, 2195
BseCI ATCGAT 1 cut(s) 2358
BseDI CCNNGG 3 cut(s) 790, 1722, 1918
BseGI GGATG 9 cut(s) 303, 429, 628, 859, 1273, 1685, 1788, 1825, 1928
BseJI GATNNNNATC 2 cut(s) 2127, 2312
BseLI CCNNNNNNNGG 5 cut(s) 496, 730, 2194, 2200, 2288
BseMI GCAATG 4 cut(s) 339, 561, 923, 1617
BseMII CTCAG 2 cut(s) 9, 1437
BseNI ACTGG 3 cut(s) 1301, 1574, 2299
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 5 cut(s) 337, 1025, 1147, 2040, 2477
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 2484
BshFI GGCC 3 cut(s) 224, 510, 1436
BshNI GGYRCC 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 2358
BsiHKCI CYCGRG 1 cut(s) 1917
BsiSI CCGG 3 cut(s) 8, 165, 421
BslFI GGGAC 1 cut(s) 2217
BslI CCNNNNNNNGG 5 cut(s) 496, 730, 2194, 2200, 2288
BsmAI GTCTC 3 cut(s) 48, 1052, 2091
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 2217
BsnI GGCC 3 cut(s) 224, 510, 1436
Bso31I GGTCTC 1 cut(s) 2091
BsoBI CYCGRG 1 cut(s) 1917
Bsp13I TCCGGA 1 cut(s) 164
BspACI CCGC 6 cut(s) 522, 939, 1216, 1856, 1862, 2200
BspANI GGCC 3 cut(s) 224, 510, 1436
BspCNI CTCAG 2 cut(s) 10, 1438
BspDI ATCGAT 1 cut(s) 2358
BspEI TCCGGA 1 cut(s) 164
BspHI TCATGA 2 cut(s) 1099, 1834
BspLI GGNNCC 3 cut(s) 163, 757, 1860
BspMI ACCTGC 2 cut(s) 1285, 1375
BspPI GGATC 8 cut(s) 156, 169, 701, 915, 1143, 1853, 1866, 2420
BspT107I GGYRCC 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 1487
BspTNI GGTCTC 1 cut(s) 2091
BsrBI CCGCTC 1 cut(s) 1864
BsrDI GCAATG 4 cut(s) 339, 561, 923, 1617
BsrI ACTGG 3 cut(s) 1301, 1574, 2299
BssECI CCNNGG 3 cut(s) 790, 1722, 1918
BssNAI GTATAC 1 cut(s) 2378
BssT1I CCWWGG 1 cut(s) 790
Bst1107I GTATAC 1 cut(s) 2378
Bst2UI CCWGG 4 cut(s) 596, 1724, 1901, 2195
Bst4CI ACNGT 5 cut(s) 1148, 1472, 1632, 2189, 2327
Bst6I CTCTTC 3 cut(s) 357, 765, 1398
BstAFI CTTAAG 1 cut(s) 1487
BstAPI GCANNNNNTGC 1 cut(s) 925
BstC8I GCNNGC 7 cut(s) 512, 921, 1043, 1421, 1766, 2470, 2524
BstDEI CTNAG 5 cut(s) 18, 930, 1164, 1446, 2168
BstF5I GGATG 9 cut(s) 303, 429, 628, 859, 1273, 1685, 1788, 1825, 1928
BstH2I RGCGCY 1 cut(s) 325
BstHHI GCGC 1 cut(s) 324
BstMAI GTCTC 3 cut(s) 48, 1052, 2091
BstMWI GCNNNNNNNGC 1 cut(s) 925
BstNI CCWGG 4 cut(s) 596, 1724, 1901, 2195
BstNSI RCATGY 4 cut(s) 923, 1093, 1772, 1977
BstSCI CCNGG 4 cut(s) 594, 1722, 1899, 2193
BstV1I GCAGC 5 cut(s) 337, 1025, 1147, 2040, 2477
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 2 cut(s) 161, 1858
BstYI RGATCY 2 cut(s) 161, 1858
BstZ17I GTATAC 1 cut(s) 2378
Bsu15I ATCGAT 1 cut(s) 2358
Bsu36I CCTNAGG 1 cut(s) 930
BsuI GTATCC 1 cut(s) 960
BsuRI GGCC 3 cut(s) 224, 510, 1436
BsuTUI ATCGAT 1 cut(s) 2358
BtsCI GGATG 9 cut(s) 303, 429, 628, 859, 1273, 1685, 1788, 1825, 1928
BtsI GCAGTG 1 cut(s) 1933
BtsIMutI CAGTG 5 cut(s) 1308, 1709, 1933, 2323, 2366
BveI ACCTGC 2 cut(s) 1285, 1375
Cac8I GCNNGC 7 cut(s) 512, 921, 1043, 1421, 1766, 2470, 2524
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 2 cut(s) 1099, 1834
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 3 cut(s) 508, 589, 1956
ClaI ATCGAT 1 cut(s) 2358
CseI GACGC 2 cut(s) 65, 224
CsiI ACCWGGT 2 cut(s) 594, 2193
Csp6I GTAC 4 cut(s) 389, 897, 1473, 2292
CspCI CAANNNNNGTGG 2 cut(s) 1794, 1829
CviQI GTAC 4 cut(s) 389, 897, 1473, 2292
DdeI CTNAG 5 cut(s) 18, 930, 1164, 1446, 2168
DriI GACNNNNNGTC 2 cut(s) 61, 1207
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 3 cut(s) 357, 765, 1398
Eam1105I GACNNNNNGTC 2 cut(s) 61, 1207
EarI CTCTTC 3 cut(s) 357, 765, 1398
Eco130I CCWWGG 1 cut(s) 790
Eco31I GGTCTC 1 cut(s) 2091
Eco47I GGWCC 2 cut(s) 589, 1956
Eco57I CTGAAG 1 cut(s) 1879
Eco81I CCTNAGG 1 cut(s) 930
Eco88I CYCGRG 1 cut(s) 1917
EcoO109I RGGNCCY 2 cut(s) 508, 1956
EcoRI GAATTC 2 cut(s) 1295, 1408
EcoRII CCWGG 4 cut(s) 594, 1722, 1899, 2193
EcoT14I CCWWGG 1 cut(s) 790
EcoT22I ATGCAT 1 cut(s) 925
ErhI CCWWGG 1 cut(s) 790
Esp3I CGTCTC 1 cut(s) 48
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 2217
FauI CCCGC 1 cut(s) 2193
FbaI TGATCA 2 cut(s) 439, 474
FblI GTMKAC 2 cut(s) 1150, 2377
Fnu4HI GCNGC 5 cut(s) 351, 1039, 1161, 2029, 2466
FokI GGATG 9 cut(s) 290, 436, 635, 866, 1280, 1692, 1795, 1812, 1935
Fsp4HI GCNGC 5 cut(s) 351, 1039, 1161, 2029, 2466
FspBI CTAG 7 cut(s) 786, 963, 1172, 1307, 1641, 2025, 2061
GlaI GCGC 1 cut(s) 323
GluI GCNGC 5 cut(s) 351, 1039, 1161, 2029, 2466
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 1922
HaeII RGCGCY 1 cut(s) 325
HaeIII GGCC 3 cut(s) 224, 510, 1436
HapII CCGG 3 cut(s) 8, 165, 421
HgaI GACGC 2 cut(s) 65, 224
HhaI GCGC 1 cut(s) 324
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HincII GTYRAC 2 cut(s) 1095, 2335
HindII GTYRAC 2 cut(s) 1095, 2335
HindIII AAGCTT 1 cut(s) 1484
HpaII CCGG 3 cut(s) 8, 165, 421
HphI GGTGA 7 cut(s) 449, 536, 949, 2177, 2240, 2382, 2528
Hpy166II GTNNAC 7 cut(s) 592, 684, 1095, 1151, 1331, 2335, 2378
Hpy8I GTNNAC 7 cut(s) 592, 684, 1095, 1151, 1331, 2335, 2378
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 9 cut(s) 613, 828, 1219, 1444, 1624, 1832, 2168, 2228, 2492
HpyCH4III ACNGT 5 cut(s) 1148, 1472, 1632, 2189, 2327
HpyCH4IV ACGT 2 cut(s) 2503, 2535
HpyF10VI GCNNNNNNNGC 1 cut(s) 925
HpyF3I CTNAG 5 cut(s) 18, 930, 1164, 1446, 2168
HpySE526I ACGT 2 cut(s) 2503, 2535
HspAI GCGC 1 cut(s) 322
Kpn2I TCCGGA 1 cut(s) 164
Ksp22I TGATCA 2 cut(s) 439, 474
LmnI GCTCC 3 cut(s) 1627, 1918, 2261
Lsp1109I GCAGC 5 cut(s) 337, 1025, 1147, 2040, 2477
LweI GCATC 4 cut(s) 1825, 2040, 2273, 2389
MabI ACCWGGT 2 cut(s) 594, 2193
MaeI CTAG 7 cut(s) 786, 963, 1172, 1307, 1641, 2025, 2061
MaeII ACGT 2 cut(s) 2503, 2535
MbiI CCGCTC 1 cut(s) 1864
MfeI CAATTG 2 cut(s) 546, 2347
MflI RGATCY 2 cut(s) 161, 1858
MlyI GAGTC 3 cut(s) 71, 243, 1217
MmeI TCCRAC 4 cut(s) 636, 711, 1236, 1614
Mph1103I ATGCAT 1 cut(s) 925
MroI TCCGGA 1 cut(s) 164
MroXI GAANNNNTTC 2 cut(s) 1251, 1892
MseI TTAA 9 cut(s) 210, 870, 1017, 1119, 1131, 1374, 1488, 2052, 2076
MslI CAYNNNNRTG 3 cut(s) 1319, 1678, 1781
MspA1I CMGCKG 1 cut(s) 1764
MspCI CTTAAG 1 cut(s) 1487
MspI CCGG 3 cut(s) 8, 165, 421
MspR9I CCNGG 4 cut(s) 596, 1724, 1901, 2195
MunI CAATTG 2 cut(s) 546, 2347
MvaI CCWGG 4 cut(s) 596, 1724, 1901, 2195
MwoI GCNNNNNNNGC 1 cut(s) 925
NlaIV GGNNCC 3 cut(s) 163, 757, 1860
NmeAIII GCCGAG 2 cut(s) 250, 257
NmuCI GTSAC 5 cut(s) 524, 709, 955, 1199, 2189
NsiI ATGCAT 1 cut(s) 925
NspI RCATGY 4 cut(s) 923, 1093, 1772, 1977
PaeI GCATGC 1 cut(s) 923
PagI TCATGA 2 cut(s) 1099, 1834
PciI ACATGT 1 cut(s) 1089
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 222
PdmI GAANNNNTTC 2 cut(s) 1251, 1892
PfeI GAWTC 9 cut(s) 427, 1009, 1179, 1195, 1556, 2104, 2182, 2360, 2384
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 5 cut(s) 352, 1040, 1162, 2030, 2467
PleI GAGTC 3 cut(s) 70, 242, 1216
PpsI GAGTC 3 cut(s) 70, 242, 1216
PpuMI RGGWCCY 1 cut(s) 1956
PscI ACATGT 1 cut(s) 1089
PsiI TTATAA 1 cut(s) 1541
Psp5II RGGWCCY 1 cut(s) 1956
Psp6I CCWGG 4 cut(s) 594, 1722, 1899, 2193
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 4 cut(s) 594, 1722, 1899, 2193
PspN4I GGNNCC 3 cut(s) 163, 757, 1860
PspPI GGNCC 3 cut(s) 508, 589, 1956
PspPPI RGGWCCY 1 cut(s) 1956
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 2 cut(s) 161, 1858
PvuII CAGCTG 1 cut(s) 1764
RsaI GTAC 4 cut(s) 390, 898, 1474, 2293
RsaNI GTAC 4 cut(s) 389, 897, 1473, 2292
RseI CAYNNNNRTG 3 cut(s) 1319, 1678, 1781
SaqAI TTAA 9 cut(s) 210, 870, 1017, 1119, 1131, 1374, 1488, 2052, 2076
SatI GCNGC 5 cut(s) 351, 1039, 1161, 2029, 2466
Sau96I GGNCC 3 cut(s) 508, 589, 1956
ScaI AGTACT 1 cut(s) 1474
SchI GAGTC 3 cut(s) 71, 243, 1217
ScrFI CCNGG 4 cut(s) 596, 1724, 1901, 2195
SexAI ACCWGGT 2 cut(s) 594, 2193
SfaNI GCATC 4 cut(s) 1825, 2040, 2273, 2389
SinI GGWCC 2 cut(s) 589, 1956
SmiMI CAYNNNNRTG 3 cut(s) 1319, 1678, 1781
SmlI CTYRAG 3 cut(s) 182, 1487, 1794
SmoI CTYRAG 3 cut(s) 182, 1487, 1794
SphI GCATGC 1 cut(s) 923
SsiI CCGC 6 cut(s) 522, 939, 1216, 1856, 1862, 2200
SspI AATATT 1 cut(s) 1846
SspMI CTAG 7 cut(s) 786, 963, 1172, 1307, 1641, 2025, 2061
StyD4I CCNGG 4 cut(s) 594, 1722, 1899, 2193
StyI CCWWGG 1 cut(s) 790
TaaI ACNGT 5 cut(s) 1148, 1472, 1632, 2189, 2327
TaiI ACGT 2 cut(s) 2506, 2538
TaqI TCGA 7 cut(s) 52, 460, 765, 1705, 2180, 2214, 2358
TatI WGTACW 3 cut(s) 388, 896, 1472
TfiI GAWTC 9 cut(s) 427, 1009, 1179, 1195, 1556, 2104, 2182, 2360, 2384
Tru1I TTAA 9 cut(s) 210, 870, 1017, 1119, 1131, 1374, 1488, 2052, 2076
Tru9I TTAA 9 cut(s) 210, 870, 1017, 1119, 1131, 1374, 1488, 2052, 2076
TscAI CASTG 5 cut(s) 1308, 1716, 1940, 2330, 2373
TseFI GTSAC 5 cut(s) 524, 709, 955, 1199, 2189
TseI GCWGC 5 cut(s) 350, 1038, 1160, 2028, 2465
Tsp45I GTSAC 5 cut(s) 524, 709, 955, 1199, 2189
TspGWI ACGGA 3 cut(s) 543, 1207, 2097
TspRI CASTG 5 cut(s) 1308, 1716, 1940, 2330, 2373
Van91I CCANNNNNTGG 1 cut(s) 730
Vha464I CTTAAG 1 cut(s) 1487
VpaK11BI GGWCC 2 cut(s) 589, 1956
XapI RAATTY 5 cut(s) 371, 1295, 1358, 1408, 1518
XceI RCATGY 4 cut(s) 923, 1093, 1772, 1977
XmiI GTMKAC 2 cut(s) 1150, 2377
XmnI GAANNNNTTC 2 cut(s) 1251, 1892
XspI CTAG 7 cut(s) 786, 963, 1172, 1307, 1641, 2025, 2061
ZrmI AGTACT 1 cut(s) 1474
Zsp2I ATGCAT 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.