NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 55, 578 |
| AccI | GTMKAC | 1 cut(s) | 151 |
| AciI | CCGC | 2 cut(s) | 280, 644 |
| AclWI | GGATC | 2 cut(s) | 271, 401 |
| AcsI | RAATTY | 1 cut(s) | 510 |
| AcuI | CTGAAG | 2 cut(s) | 566, 752 |
| AfaI | GTAC | 3 cut(s) | 228, 255, 696 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 661, 717 |
| AgsI | TTSAA | 5 cut(s) | 73, 212, 220, 233, 542 |
| AjnI | CCWGG | 1 cut(s) | 711 |
| AluBI | AGCT | 4 cut(s) | 424, 548, 628, 723 |
| AluI | AGCT | 4 cut(s) | 424, 548, 628, 723 |
| Alw21I | GWGCWC | 3 cut(s) | 366, 550, 610 |
| Alw26I | GTCTC | 3 cut(s) | 178, 263, 477 |
| Alw44I | GTGCAC | 1 cut(s) | 606 |
| AlwI | GGATC | 2 cut(s) | 271, 401 |
| ApaLI | GTGCAC | 1 cut(s) | 606 |
| ApeKI | GCWGC | 4 cut(s) | 133, 161, 628, 727 |
| ApoI | RAATTY | 1 cut(s) | 510 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 764 |
| AspLEI | GCGC | 1 cut(s) | 175 |
| AsuHPI | GGTGA | 2 cut(s) | 644, 700 |
| BaeGI | GKGCMC | 1 cut(s) | 610 |
| BanII | GRGCYC | 1 cut(s) | 550 |
| BauI | CACGAG | 1 cut(s) | 567 |
| Bbv12I | GWGCWC | 3 cut(s) | 366, 550, 610 |
| BbvI | GCAGC | 4 cut(s) | 120, 148, 615, 714 |
| BccI | CCATC | 2 cut(s) | 188, 418 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 22, 56 |
| BciT130I | CCWGG | 1 cut(s) | 713 |
| BciVI | GTATCC | 1 cut(s) | 11 |
| BclI | TGATCA | 1 cut(s) | 274 |
| BcoDI | GTCTC | 3 cut(s) | 178, 263, 477 |
| BfuAI | ACCTGC | 2 cut(s) | 55, 578 |
| BfuI | GTATCC | 1 cut(s) | 11 |
| BisI | GCNGC | 5 cut(s) | 134, 162, 629, 645, 728 |
| BlpI | GCTNAGC | 1 cut(s) | 365 |
| BlsI | GCNGC | 5 cut(s) | 135, 163, 630, 646, 729 |
| BmcAI | AGTACT | 1 cut(s) | 228 |
| Bme1390I | CCNGG | 1 cut(s) | 713 |
| BmrFI | CCNGG | 1 cut(s) | 713 |
| BmsI | GCATC | 1 cut(s) | 340 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 382, 414 |
| BpmI | CTGGAG | 2 cut(s) | 409, 644 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 365 |
| BpuEI | CTTGAG | 1 cut(s) | 539 |
| Bsa29I | ATCGAT | 1 cut(s) | 453 |
| BsaJI | CCNNGG | 2 cut(s) | 655, 711 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 661, 717 |
| Bse1I | ACTGG | 3 cut(s) | 392, 497, 627 |
| Bse3DI | GCAATG | 2 cut(s) | 200, 360 |
| BseBI | CCWGG | 1 cut(s) | 713 |
| BseCI | ATCGAT | 1 cut(s) | 453 |
| BseDI | CCNNGG | 2 cut(s) | 655, 711 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 661, 717 |
| BseMI | GCAATG | 2 cut(s) | 200, 360 |
| BseMII | CTCAG | 3 cut(s) | 169, 379, 513 |
| BseNI | ACTGG | 3 cut(s) | 392, 497, 627 |
| BseRI | GAGGAG | 1 cut(s) | 389 |
| BseSI | GKGCMC | 1 cut(s) | 610 |
| BseXI | GCAGC | 4 cut(s) | 120, 148, 615, 714 |
| BshVI | ATCGAT | 1 cut(s) | 453 |
| BsiHKAI | GWGCWC | 3 cut(s) | 366, 550, 610 |
| BsiSI | CCGG | 1 cut(s) | 137 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 661, 717 |
| BsmAI | GTCTC | 3 cut(s) | 178, 263, 477 |
| BsmI | GAATGC | 1 cut(s) | 764 |
| Bsp1286I | GDGCHC | 3 cut(s) | 366, 550, 610 |
| Bsp1407I | TGTACA | 1 cut(s) | 694 |
| Bsp143I | GATC | 3 cut(s) | 263, 274, 406 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 365 |
| BspACI | CCGC | 2 cut(s) | 280, 644 |
| BspCNI | CTCAG | 3 cut(s) | 168, 378, 512 |
| BspDI | ATCGAT | 1 cut(s) | 453 |
| BspHI | TCATGA | 1 cut(s) | 288 |
| BspMI | ACCTGC | 2 cut(s) | 55, 578 |
| BspPI | GGATC | 2 cut(s) | 271, 401 |
| BsrDI | GCAATG | 2 cut(s) | 200, 360 |
| BsrGI | TGTACA | 1 cut(s) | 694 |
| BsrI | ACTGG | 3 cut(s) | 392, 497, 627 |
| BssECI | CCNNGG | 2 cut(s) | 655, 711 |
| BssMI | GATC | 3 cut(s) | 263, 274, 406 |
| BssSI | CACGAG | 1 cut(s) | 567 |
| Bst2BI | CACGAG | 1 cut(s) | 567 |
| Bst2UI | CCWGG | 1 cut(s) | 713 |
| Bst4CI | ACNGT | 3 cut(s) | 65, 656, 754 |
| Bst6I | CTCTTC | 2 cut(s) | 176, 568 |
| BstAUI | TGTACA | 1 cut(s) | 694 |
| BstC8I | GCNNGC | 3 cut(s) | 282, 606, 725 |
| BstDEI | CTNAG | 4 cut(s) | 155, 365, 479, 499 |
| BstDSI | CCRYGG | 1 cut(s) | 655 |
| BstHHI | GCGC | 1 cut(s) | 175 |
| BstKTI | GATC | 3 cut(s) | 266, 277, 409 |
| BstMAI | GTCTC | 3 cut(s) | 178, 263, 477 |
| BstMBI | GATC | 3 cut(s) | 263, 274, 406 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 56, 170, 733 |
| BstNI | CCWGG | 1 cut(s) | 713 |
| BstNSI | RCATGY | 1 cut(s) | 191 |
| BstSCI | CCNGG | 1 cut(s) | 711 |
| BstSLI | GKGCMC | 1 cut(s) | 610 |
| BstV1I | GCAGC | 4 cut(s) | 120, 148, 615, 714 |
| Bsu15I | ATCGAT | 1 cut(s) | 453 |
| BsuI | GTATCC | 1 cut(s) | 11 |
| BsuTUI | ATCGAT | 1 cut(s) | 453 |
| BtgI | CCRYGG | 1 cut(s) | 655 |
| BtsIMutI | CAGTG | 3 cut(s) | 61, 507, 759 |
| BveI | ACCTGC | 2 cut(s) | 55, 578 |
| Cac8I | GCNNGC | 3 cut(s) | 282, 606, 725 |
| CciI | TCATGA | 1 cut(s) | 288 |
| CfoI | GCGC | 1 cut(s) | 175 |
| ClaI | ATCGAT | 1 cut(s) | 453 |
| Csp6I | GTAC | 3 cut(s) | 227, 254, 695 |
| CviAII | CATG | 5 cut(s) | 188, 289, 370, 475, 551 |
| CviQI | GTAC | 3 cut(s) | 227, 254, 695 |
| DdeI | CTNAG | 4 cut(s) | 155, 365, 479, 499 |
| DpnI | GATC | 3 cut(s) | 265, 276, 408 |
| DpnII | GATC | 3 cut(s) | 263, 274, 406 |
| Eam1104I | CTCTTC | 2 cut(s) | 176, 568 |
| EarI | CTCTTC | 2 cut(s) | 176, 568 |
| Ecl136II | GAGCTC | 1 cut(s) | 548 |
| Eco24I | GRGCYC | 1 cut(s) | 550 |
| Eco53kI | GAGCTC | 1 cut(s) | 548 |
| Eco57I | CTGAAG | 2 cut(s) | 566, 752 |
| EcoICRI | GAGCTC | 1 cut(s) | 548 |
| EcoRI | GAATTC | 1 cut(s) | 510 |
| EcoRII | CCWGG | 1 cut(s) | 711 |
| EcoT38I | GRGCYC | 1 cut(s) | 550 |
| FaeI | CATG | 5 cut(s) | 191, 292, 373, 478, 554 |
| FatI | CATG | 5 cut(s) | 187, 288, 369, 474, 550 |
| FauI | CCCGC | 1 cut(s) | 273 |
| FbaI | TGATCA | 1 cut(s) | 274 |
| FblI | GTMKAC | 1 cut(s) | 151 |
| Fnu4HI | GCNGC | 5 cut(s) | 134, 162, 629, 645, 728 |
| FriOI | GRGCYC | 1 cut(s) | 550 |
| Fsp4HI | GCNGC | 5 cut(s) | 134, 162, 629, 645, 728 |
| GlaI | GCGC | 1 cut(s) | 174 |
| GluI | GCNGC | 5 cut(s) | 134, 162, 629, 645, 728 |
| GsuI | CTGGAG | 2 cut(s) | 409, 644 |
| HapII | CCGG | 1 cut(s) | 137 |
| HhaI | GCGC | 1 cut(s) | 175 |
| Hin1II | CATG | 5 cut(s) | 191, 292, 373, 478, 554 |
| Hin6I | GCGC | 1 cut(s) | 173 |
| HinP1I | GCGC | 1 cut(s) | 173 |
| HincII | GTYRAC | 1 cut(s) | 687 |
| HindII | GTYRAC | 1 cut(s) | 687 |
| HinfI | GANTC | 3 cut(s) | 153, 560, 576 |
| HpaII | CCGG | 1 cut(s) | 137 |
| HphI | GGTGA | 2 cut(s) | 644, 700 |
| Hpy166II | GTNNAC | 4 cut(s) | 152, 608, 687, 708 |
| Hpy188I | TCNGA | 3 cut(s) | 87, 240, 268 |
| Hpy188III | TCNNGA | 3 cut(s) | 289, 539, 599 |
| Hpy8I | GTNNAC | 4 cut(s) | 152, 608, 687, 708 |
| HpyAV | CCTTC | 8 cut(s) | 134, 138, 447, 527, 590, 629, 673, 754 |
| HpyCH4III | ACNGT | 3 cut(s) | 65, 656, 754 |
| HpyCH4V | TGCA | 6 cut(s) | 12, 50, 317, 353, 448, 608 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 56, 170, 733 |
| HpyF3I | CTNAG | 4 cut(s) | 155, 365, 479, 499 |
| Hsp92II | CATG | 5 cut(s) | 191, 292, 373, 478, 554 |
| HspAI | GCGC | 1 cut(s) | 173 |
| Ksp22I | TGATCA | 1 cut(s) | 274 |
| Kzo9I | GATC | 3 cut(s) | 263, 274, 406 |
| LmnI | GCTCC | 3 cut(s) | 169, 625, 720 |
| Lsp1109I | GCAGC | 4 cut(s) | 120, 148, 615, 714 |
| LweI | GCATC | 1 cut(s) | 340 |
| MaeIII | GTNAC | 3 cut(s) | 303, 383, 805 |
| MalI | GATC | 3 cut(s) | 265, 276, 408 |
| MboI | GATC | 3 cut(s) | 263, 274, 406 |
| MboII | GAAGA | 6 cut(s) | 48, 193, 224, 259, 555, 786 |
| MfeI | CAATTG | 1 cut(s) | 207 |
| MhlI | GDGCHC | 3 cut(s) | 366, 550, 610 |
| MluCI | AATT | 4 cut(s) | 207, 340, 510, 790 |
| MlyI | GAGTC | 3 cut(s) | 147, 554, 570 |
| MmeI | TCCRAC | 1 cut(s) | 191 |
| MnlI | CCTC | 5 cut(s) | 32, 367, 370, 384, 736 |
| MroXI | GAANNNNTTC | 1 cut(s) | 764 |
| MseI | TTAA | 2 cut(s) | 330, 420 |
| MslI | CAYNNNNRTG | 3 cut(s) | 192, 312, 803 |
| MspA1I | CMGCKG | 1 cut(s) | 280 |
| MspI | CCGG | 1 cut(s) | 137 |
| MspR9I | CCNGG | 1 cut(s) | 713 |
| MunI | CAATTG | 1 cut(s) | 207 |
| Mva1269I | GAATGC | 1 cut(s) | 764 |
| MvaI | CCWGG | 1 cut(s) | 713 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 56, 170, 733 |
| NdeII | GATC | 3 cut(s) | 263, 274, 406 |
| NlaIII | CATG | 5 cut(s) | 191, 292, 373, 478, 554 |
| NmuCI | GTSAC | 1 cut(s) | 805 |
| NspI | RCATGY | 1 cut(s) | 191 |
| PagI | TCATGA | 1 cut(s) | 288 |
| PctI | GAATGC | 1 cut(s) | 764 |
| PdmI | GAANNNNTTC | 1 cut(s) | 764 |
| PkrI | GCNGC | 5 cut(s) | 135, 163, 630, 646, 729 |
| PleI | GAGTC | 3 cut(s) | 147, 554, 570 |
| PpsI | GAGTC | 3 cut(s) | 147, 554, 570 |
| Psp124BI | GAGCTC | 1 cut(s) | 550 |
| Psp6I | CCWGG | 1 cut(s) | 711 |
| PspGI | CCWGG | 1 cut(s) | 711 |
| RsaI | GTAC | 3 cut(s) | 228, 255, 696 |
| RsaNI | GTAC | 3 cut(s) | 227, 254, 695 |
| RseI | CAYNNNNRTG | 3 cut(s) | 192, 312, 803 |
| SacI | GAGCTC | 1 cut(s) | 550 |
| SaqAI | TTAA | 2 cut(s) | 330, 420 |
| SatI | GCNGC | 5 cut(s) | 134, 162, 629, 645, 728 |
| Sau3AI | GATC | 3 cut(s) | 263, 274, 406 |
| ScaI | AGTACT | 1 cut(s) | 228 |
| SchI | GAGTC | 3 cut(s) | 147, 554, 570 |
| ScrFI | CCNGG | 1 cut(s) | 713 |
| SduI | GDGCHC | 3 cut(s) | 366, 550, 610 |
| SfaNI | GCATC | 1 cut(s) | 340 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 192, 312, 803 |
| SmlI | CTYRAG | 1 cut(s) | 518 |
| SmoI | CTYRAG | 1 cut(s) | 518 |
| Sse9I | AATT | 4 cut(s) | 207, 340, 510, 790 |
| SsiI | CCGC | 2 cut(s) | 280, 644 |
| SstI | GAGCTC | 1 cut(s) | 550 |
| StyD4I | CCNGG | 1 cut(s) | 711 |
| TaaI | ACNGT | 3 cut(s) | 65, 656, 754 |
| TaqI | TCGA | 5 cut(s) | 93, 179, 429, 453, 768 |
| TasI | AATT | 4 cut(s) | 207, 340, 510, 790 |
| TatI | WGTACW | 3 cut(s) | 226, 253, 694 |
| TauI | GCSGC | 1 cut(s) | 647 |
| Tru1I | TTAA | 2 cut(s) | 330, 420 |
| Tru9I | TTAA | 2 cut(s) | 330, 420 |
| TscAI | CASTG | 3 cut(s) | 68, 507, 759 |
| TseFI | GTSAC | 1 cut(s) | 805 |
| TseI | GCWGC | 4 cut(s) | 133, 161, 628, 727 |
| Tsp45I | GTSAC | 1 cut(s) | 805 |
| TspDTI | ATGAA | 3 cut(s) | 189, 463, 692 |
| TspRI | CASTG | 3 cut(s) | 68, 507, 759 |
| VneI | GTGCAC | 1 cut(s) | 606 |
| XapI | RAATTY | 1 cut(s) | 510 |
| XceI | RCATGY | 1 cut(s) | 191 |
| XmiI | GTMKAC | 1 cut(s) | 151 |
| XmnI | GAANNNNTTC | 1 cut(s) | 764 |
| ZrmI | AGTACT | 1 cut(s) | 228 |