NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 661 |
| AccI | GTMKAC | 4 cut(s) | 51, 251, 266, 643 |
| AciI | CCGC | 3 cut(s) | 210, 596, 610 |
| AcoI | YGGCCR | 1 cut(s) | 337 |
| AcsI | RAATTY | 2 cut(s) | 132, 757 |
| AcuI | CTGAAG | 3 cut(s) | 189, 424, 882 |
| AfaI | GTAC | 2 cut(s) | 230, 570 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 209, 694 |
| AgsI | TTSAA | 7 cut(s) | 164, 398, 512, 560, 771, 810, 904 |
| AjnI | CCWGG | 1 cut(s) | 339 |
| AluBI | AGCT | 6 cut(s) | 46, 170, 310, 563, 605, 872 |
| AluI | AGCT | 6 cut(s) | 46, 170, 310, 563, 605, 872 |
| Alw21I | GWGCWC | 1 cut(s) | 172 |
| Alw26I | GTCTC | 2 cut(s) | 122, 295 |
| Ama87I | CYCGRG | 1 cut(s) | 652 |
| AoxI | GGCC | 1 cut(s) | 337 |
| ApeKI | GCWGC | 2 cut(s) | 310, 680 |
| ApoI | RAATTY | 2 cut(s) | 132, 757 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 625, 657 |
| AspS9I | GGNCC | 2 cut(s) | 628, 803 |
| AsuHPI | GGTGA | 1 cut(s) | 128 |
| AvaI | CYCGRG | 1 cut(s) | 652 |
| AvaII | GGWCC | 2 cut(s) | 628, 803 |
| BalI | TGGCCA | 1 cut(s) | 339 |
| BanI | GGYRCC | 1 cut(s) | 661 |
| BanII | GRGCYC | 1 cut(s) | 172 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 552, 584 |
| BauI | CACGAG | 1 cut(s) | 189 |
| Bbv12I | GWGCWC | 1 cut(s) | 172 |
| BbvI | GCAGC | 2 cut(s) | 297, 667 |
| BciT130I | CCWGG | 1 cut(s) | 341 |
| BcoDI | GTCTC | 2 cut(s) | 122, 295 |
| BfaI | CTAG | 3 cut(s) | 47, 869, 911 |
| BfmI | CTRYAG | 1 cut(s) | 705 |
| BisI | GCNGC | 2 cut(s) | 311, 681 |
| BlsI | GCNGC | 2 cut(s) | 312, 682 |
| Bme1390I | CCNGG | 1 cut(s) | 341 |
| Bme18I | GGWCC | 2 cut(s) | 628, 803 |
| BmeT110I | CYCGRG | 1 cut(s) | 652 |
| BmgT120I | GGNCC | 2 cut(s) | 628, 803 |
| BmiI | GGNNCC | 1 cut(s) | 663 |
| BmrFI | CCNGG | 1 cut(s) | 341 |
| BmsI | GCATC | 6 cut(s) | 117, 442, 501, 703, 826, 833 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 34 |
| BpmI | CTGGAG | 1 cut(s) | 326 |
| BpuEI | CTTGAG | 2 cut(s) | 161, 858 |
| Bsa29I | ATCGAT | 1 cut(s) | 75 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 209, 694 |
| Bse1I | ACTGG | 1 cut(s) | 309 |
| Bse3DI | GCAATG | 1 cut(s) | 750 |
| BseBI | CCWGG | 1 cut(s) | 341 |
| BseCI | ATCGAT | 1 cut(s) | 75 |
| BseGI | GGATG | 2 cut(s) | 477, 886 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 209, 694 |
| BseMI | GCAATG | 1 cut(s) | 750 |
| BseMII | CTCAG | 2 cut(s) | 135, 684 |
| BseNI | ACTGG | 1 cut(s) | 309 |
| BseRI | GAGGAG | 1 cut(s) | 23 |
| BseXI | GCAGC | 2 cut(s) | 297, 667 |
| BseYI | CCCAGC | 1 cut(s) | 537 |
| BsgI | GTGCAG | 1 cut(s) | 357 |
| Bsh1285I | CGRYCG | 1 cut(s) | 600 |
| BshFI | GGCC | 1 cut(s) | 339 |
| BshNI | GGYRCC | 1 cut(s) | 661 |
| BshVI | ATCGAT | 1 cut(s) | 75 |
| BsiEI | CGRYCG | 1 cut(s) | 600 |
| BsiHKAI | GWGCWC | 1 cut(s) | 172 |
| BsiHKCI | CYCGRG | 1 cut(s) | 652 |
| BslFI | GGGAC | 2 cut(s) | 634, 669 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 209, 694 |
| BsmAI | GTCTC | 2 cut(s) | 122, 295 |
| BsmFI | GGGAC | 2 cut(s) | 634, 669 |
| BsnI | GGCC | 1 cut(s) | 339 |
| BsoBI | CYCGRG | 1 cut(s) | 652 |
| Bsp1286I | GDGCHC | 1 cut(s) | 172 |
| Bsp143I | GATC | 2 cut(s) | 28, 587 |
| BspACI | CCGC | 3 cut(s) | 210, 596, 610 |
| BspANI | GGCC | 1 cut(s) | 339 |
| BspCNI | CTCAG | 2 cut(s) | 134, 685 |
| BspDI | ATCGAT | 1 cut(s) | 75 |
| BspLI | GGNNCC | 1 cut(s) | 663 |
| BspMAI | CTGCAG | 1 cut(s) | 709 |
| BspT107I | GGYRCC | 1 cut(s) | 661 |
| BsrDI | GCAATG | 1 cut(s) | 750 |
| BsrI | ACTGG | 1 cut(s) | 309 |
| BssMI | GATC | 2 cut(s) | 28, 587 |
| BssSI | CACGAG | 1 cut(s) | 189 |
| Bst2BI | CACGAG | 1 cut(s) | 189 |
| Bst2UI | CCWGG | 1 cut(s) | 341 |
| Bst4CI | ACNGT | 1 cut(s) | 116 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 704, 713 |
| BstC8I | GCNNGC | 1 cut(s) | 392 |
| BstDEI | CTNAG | 4 cut(s) | 101, 121, 257, 693 |
| BstF5I | GGATG | 2 cut(s) | 477, 886 |
| BstKTI | GATC | 2 cut(s) | 31, 590 |
| BstMAI | GTCTC | 2 cut(s) | 122, 295 |
| BstMBI | GATC | 2 cut(s) | 28, 587 |
| BstMCI | CGRYCG | 1 cut(s) | 600 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 400, 602, 704, 713, 781 |
| BstNI | CCWGG | 1 cut(s) | 341 |
| BstSCI | CCNGG | 1 cut(s) | 339 |
| BstSFI | CTRYAG | 1 cut(s) | 705 |
| BstV1I | GCAGC | 2 cut(s) | 297, 667 |
| Bsu15I | ATCGAT | 1 cut(s) | 75 |
| BsuRI | GGCC | 1 cut(s) | 339 |
| BsuTUI | ATCGAT | 1 cut(s) | 75 |
| BtgZI | GCGATG | 1 cut(s) | 518 |
| BtsCI | GGATG | 2 cut(s) | 477, 886 |
| BtsIMutI | CAGTG | 2 cut(s) | 129, 340 |
| Cac8I | GCNNGC | 1 cut(s) | 392 |
| Cfr13I | GGNCC | 2 cut(s) | 628, 803 |
| ClaI | ATCGAT | 1 cut(s) | 75 |
| Csp6I | GTAC | 2 cut(s) | 229, 569 |
| CviAII | CATG | 4 cut(s) | 97, 173, 433, 574 |
| CviQI | GTAC | 2 cut(s) | 229, 569 |
| DdeI | CTNAG | 4 cut(s) | 101, 121, 257, 693 |
| DpnI | GATC | 2 cut(s) | 30, 589 |
| DpnII | GATC | 2 cut(s) | 28, 587 |
| EaeI | YGGCCR | 1 cut(s) | 337 |
| Ecl136II | GAGCTC | 1 cut(s) | 170 |
| Eco24I | GRGCYC | 1 cut(s) | 172 |
| Eco47I | GGWCC | 2 cut(s) | 628, 803 |
| Eco53kI | GAGCTC | 1 cut(s) | 170 |
| Eco57I | CTGAAG | 3 cut(s) | 189, 424, 882 |
| Eco88I | CYCGRG | 1 cut(s) | 652 |
| EcoICRI | GAGCTC | 1 cut(s) | 170 |
| EcoO109I | RGGNCCY | 1 cut(s) | 803 |
| EcoRI | GAATTC | 1 cut(s) | 132 |
| EcoRII | CCWGG | 1 cut(s) | 339 |
| EcoT22I | ATGCAT | 1 cut(s) | 826 |
| EcoT38I | GRGCYC | 1 cut(s) | 172 |
| FaeI | CATG | 4 cut(s) | 100, 176, 436, 577 |
| FaqI | GGGAC | 2 cut(s) | 634, 669 |
| FatI | CATG | 4 cut(s) | 96, 172, 432, 573 |
| FauI | CCCGC | 2 cut(s) | 203, 603 |
| FblI | GTMKAC | 4 cut(s) | 51, 251, 266, 643 |
| Fnu4HI | GCNGC | 2 cut(s) | 311, 681 |
| FokI | GGATG | 2 cut(s) | 484, 893 |
| FriOI | GRGCYC | 1 cut(s) | 172 |
| Fsp4HI | GCNGC | 2 cut(s) | 311, 681 |
| FspBI | CTAG | 3 cut(s) | 47, 869, 911 |
| GluI | GCNGC | 2 cut(s) | 311, 681 |
| GsaI | CCCAGC | 1 cut(s) | 541 |
| GsuI | CTGGAG | 1 cut(s) | 326 |
| HaeIII | GGCC | 1 cut(s) | 339 |
| Hin1II | CATG | 4 cut(s) | 100, 176, 436, 577 |
| HincII | GTYRAC | 3 cut(s) | 52, 366, 644 |
| HindII | GTYRAC | 3 cut(s) | 52, 366, 644 |
| HindIII | AAGCTT | 1 cut(s) | 603 |
| HinfI | GANTC | 7 cut(s) | 11, 182, 199, 281, 410, 512, 551 |
| HphI | GGTGA | 1 cut(s) | 128 |
| Hpy166II | GTNNAC | 7 cut(s) | 52, 229, 252, 267, 366, 527, 644 |
| Hpy188I | TCNGA | 6 cut(s) | 241, 286, 443, 694, 736, 829 |
| Hpy188III | TCNNGA | 6 cut(s) | 161, 222, 482, 509, 654, 911 |
| Hpy8I | GTNNAC | 7 cut(s) | 52, 229, 252, 267, 366, 527, 644 |
| HpyAV | CCTTC | 8 cut(s) | 69, 149, 213, 311, 352, 816, 843, 910 |
| HpyCH4III | ACNGT | 1 cut(s) | 116 |
| HpyCH4IV | ACGT | 1 cut(s) | 812 |
| HpyCH4V | TGCA | 9 cut(s) | 70, 374, 455, 492, 707, 743, 755, 824, 839 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 400, 602, 704, 713, 781 |
| HpyF3I | CTNAG | 4 cut(s) | 101, 121, 257, 693 |
| HpySE526I | ACGT | 1 cut(s) | 812 |
| Hsp92II | CATG | 4 cut(s) | 100, 176, 436, 577 |
| Kzo9I | GATC | 2 cut(s) | 28, 587 |
| LmnI | GCTCC | 1 cut(s) | 307 |
| Lsp1109I | GCAGC | 2 cut(s) | 297, 667 |
| LweI | GCATC | 6 cut(s) | 117, 442, 501, 703, 826, 833 |
| MaeI | CTAG | 3 cut(s) | 47, 869, 911 |
| MaeII | ACGT | 1 cut(s) | 812 |
| MalI | GATC | 2 cut(s) | 30, 589 |
| MboI | GATC | 2 cut(s) | 28, 587 |
| MboII | GAAGA | 1 cut(s) | 875 |
| MhlI | GDGCHC | 1 cut(s) | 172 |
| MlsI | TGGCCA | 1 cut(s) | 339 |
| MluCI | AATT | 4 cut(s) | 36, 132, 699, 757 |
| MluNI | TGGCCA | 1 cut(s) | 339 |
| MlyI | GAGTC | 3 cut(s) | 20, 176, 193 |
| MnlI | CCTC | 6 cut(s) | 98, 462, 510, 611, 688, 794 |
| Mox20I | TGGCCA | 1 cut(s) | 339 |
| Mph1103I | ATGCAT | 1 cut(s) | 826 |
| MscI | TGGCCA | 1 cut(s) | 339 |
| MseI | TTAA | 1 cut(s) | 195 |
| Msp20I | TGGCCA | 1 cut(s) | 339 |
| MspR9I | CCNGG | 1 cut(s) | 341 |
| MvaI | CCWGG | 1 cut(s) | 341 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 400, 602, 704, 713, 781 |
| NdeII | GATC | 2 cut(s) | 28, 587 |
| NlaIII | CATG | 4 cut(s) | 100, 176, 436, 577 |
| NlaIV | GGNNCC | 1 cut(s) | 663 |
| NsiI | ATGCAT | 1 cut(s) | 826 |
| PfeI | GAWTC | 4 cut(s) | 281, 410, 512, 551 |
| PkrI | GCNGC | 2 cut(s) | 312, 682 |
| PleI | GAGTC | 3 cut(s) | 19, 176, 193 |
| PpsI | GAGTC | 3 cut(s) | 19, 176, 193 |
| PpuMI | RGGWCCY | 1 cut(s) | 803 |
| Psp124BI | GAGCTC | 1 cut(s) | 172 |
| Psp5II | RGGWCCY | 1 cut(s) | 803 |
| Psp6I | CCWGG | 1 cut(s) | 339 |
| PspFI | CCCAGC | 1 cut(s) | 537 |
| PspGI | CCWGG | 1 cut(s) | 339 |
| PspN4I | GGNNCC | 1 cut(s) | 663 |
| PspPI | GGNCC | 2 cut(s) | 628, 803 |
| PspPPI | RGGWCCY | 1 cut(s) | 803 |
| PstI | CTGCAG | 1 cut(s) | 709 |
| RsaI | GTAC | 2 cut(s) | 230, 570 |
| RsaNI | GTAC | 2 cut(s) | 229, 569 |
| SacI | GAGCTC | 1 cut(s) | 172 |
| SalI | GTCGAC | 2 cut(s) | 50, 642 |
| SaqAI | TTAA | 1 cut(s) | 195 |
| SatI | GCNGC | 2 cut(s) | 311, 681 |
| Sau3AI | GATC | 2 cut(s) | 28, 587 |
| Sau96I | GGNCC | 2 cut(s) | 628, 803 |
| SchI | GAGTC | 3 cut(s) | 20, 176, 193 |
| ScrFI | CCNGG | 1 cut(s) | 341 |
| SduI | GDGCHC | 1 cut(s) | 172 |
| SfaNI | GCATC | 6 cut(s) | 117, 442, 501, 703, 826, 833 |
| SfcI | CTRYAG | 1 cut(s) | 705 |
| SinI | GGWCC | 2 cut(s) | 628, 803 |
| SmlI | CTYRAG | 2 cut(s) | 140, 873 |
| SmoI | CTYRAG | 2 cut(s) | 140, 873 |
| Sse9I | AATT | 4 cut(s) | 36, 132, 699, 757 |
| SsiI | CCGC | 3 cut(s) | 210, 596, 610 |
| SspMI | CTAG | 3 cut(s) | 47, 869, 911 |
| SstI | GAGCTC | 1 cut(s) | 172 |
| StyD4I | CCNGG | 1 cut(s) | 339 |
| TaaI | ACNGT | 1 cut(s) | 116 |
| TaiI | ACGT | 1 cut(s) | 815 |
| TaqI | TCGA | 5 cut(s) | 51, 75, 600, 643, 711 |
| TasI | AATT | 4 cut(s) | 36, 132, 699, 757 |
| TatI | WGTACW | 1 cut(s) | 228 |
| TfiI | GAWTC | 4 cut(s) | 281, 410, 512, 551 |
| Tru1I | TTAA | 1 cut(s) | 195 |
| Tru9I | TTAA | 1 cut(s) | 195 |
| TscAI | CASTG | 2 cut(s) | 129, 340 |
| TseI | GCWGC | 2 cut(s) | 310, 680 |
| TspDTI | ATGAA | 4 cut(s) | 85, 371, 423, 876 |
| TspRI | CASTG | 2 cut(s) | 129, 340 |
| VpaK11BI | GGWCC | 2 cut(s) | 628, 803 |
| XapI | RAATTY | 2 cut(s) | 132, 757 |
| XbaI | TCTAGA | 1 cut(s) | 910 |
| XmiI | GTMKAC | 4 cut(s) | 51, 251, 266, 643 |
| XspI | CTAG | 3 cut(s) | 47, 869, 911 |
| Zsp2I | ATGCAT | 1 cut(s) | 826 |