NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 4 cut(s) | 375, 452, 552, 633 |
| AccB1I | GGYRCC | 2 cut(s) | 1103, 1271 |
| AccBSI | CCGCTC | 1 cut(s) | 659 |
| AciI | CCGC | 4 cut(s) | 122, 659, 666, 870 |
| AclWI | GGATC | 1 cut(s) | 113 |
| AcsI | RAATTY | 2 cut(s) | 307, 1367 |
| AcuI | CTGAAG | 9 cut(s) | 363, 440, 540, 621, 723, 765, 894, 982, 1150 |
| AfaI | GTAC | 6 cut(s) | 70, 97, 622, 718, 1015, 1183 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 683, 740, 1136, 1304 |
| AflIII | ACRYGT | 1 cut(s) | 623 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 917, 949 |
| Alw21I | GWGCWC | 5 cut(s) | 208, 347, 424, 524, 601 |
| Alw26I | GTCTC | 3 cut(s) | 20, 105, 274 |
| AlwI | GGATC | 1 cut(s) | 113 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 746, 1122, 1290 |
| Ama87I | CYCGRG | 2 cut(s) | 1094, 1262 |
| AoxI | GGCC | 2 cut(s) | 1021, 1189 |
| ApeKI | GCWGC | 6 cut(s) | 3, 944, 1122, 1149, 1152, 1290 |
| ApoI | RAATTY | 2 cut(s) | 307, 1367 |
| AspS9I | GGNCC | 2 cut(s) | 1070, 1238 |
| AsuHPI | GGTGA | 3 cut(s) | 303, 666, 722 |
| AvaI | CYCGRG | 2 cut(s) | 1094, 1262 |
| AvaII | GGWCC | 2 cut(s) | 1070, 1238 |
| BaeGI | GKGCMC | 1 cut(s) | 807 |
| BanI | GGYRCC | 2 cut(s) | 1103, 1271 |
| BanII | GRGCYC | 4 cut(s) | 347, 424, 524, 601 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 997, 1029, 1165, 1197 |
| Bbv12I | GWGCWC | 5 cut(s) | 208, 347, 424, 524, 601 |
| BbvI | GCAGC | 5 cut(s) | 956, 1109, 1161, 1164, 1277 |
| BccI | CCATC | 2 cut(s) | 30, 838 |
| BclI | TGATCA | 1 cut(s) | 116 |
| BcoDI | GTCTC | 3 cut(s) | 20, 105, 274 |
| BfmI | CTRYAG | 3 cut(s) | 975, 1147, 1315 |
| BfuAI | ACCTGC | 4 cut(s) | 375, 452, 552, 633 |
| BisI | GCNGC | 8 cut(s) | 4, 667, 870, 945, 1123, 1150, 1153, 1291 |
| BlpI | GCTNAGC | 1 cut(s) | 207 |
| BlsI | GCNGC | 8 cut(s) | 5, 668, 871, 946, 1124, 1151, 1154, 1292 |
| BmcAI | AGTACT | 1 cut(s) | 70 |
| Bme18I | GGWCC | 2 cut(s) | 1070, 1238 |
| BmeT110I | CYCGRG | 2 cut(s) | 1094, 1262 |
| BmgT120I | GGNCC | 2 cut(s) | 1070, 1238 |
| BmiI | GGNNCC | 2 cut(s) | 1105, 1273 |
| BmsI | GCATC | 9 cut(s) | 182, 389, 466, 489, 566, 983, 1109, 1151, 1277 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 207 |
| BpuEI | CTTGAG | 1 cut(s) | 336 |
| Bsa29I | ATCGAT | 1 cut(s) | 250 |
| BsaJI | CCNNGG | 1 cut(s) | 677 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 683, 740, 1136, 1304 |
| Bse1I | ACTGG | 1 cut(s) | 294 |
| Bse3DI | GCAATG | 3 cut(s) | 42, 202, 1360 |
| BseCI | ATCGAT | 1 cut(s) | 250 |
| BseDI | CCNNGG | 1 cut(s) | 677 |
| BseGI | GGATG | 2 cut(s) | 755, 818 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 683, 740, 1136, 1304 |
| BseMI | GCAATG | 3 cut(s) | 42, 202, 1360 |
| BseMII | CTCAG | 5 cut(s) | 221, 310, 799, 1126, 1294 |
| BseNI | ACTGG | 1 cut(s) | 294 |
| BseRI | GAGGAG | 1 cut(s) | 231 |
| BseSI | GKGCMC | 1 cut(s) | 807 |
| BseXI | GCAGC | 5 cut(s) | 956, 1109, 1161, 1164, 1277 |
| BseYI | CCCAGC | 1 cut(s) | 733 |
| BshFI | GGCC | 2 cut(s) | 1023, 1191 |
| BshNI | GGYRCC | 2 cut(s) | 1103, 1271 |
| BshVI | ATCGAT | 1 cut(s) | 250 |
| BsiHKAI | GWGCWC | 5 cut(s) | 208, 347, 424, 524, 601 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1094, 1262 |
| BslFI | GGGAC | 4 cut(s) | 1076, 1111, 1244, 1279 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 683, 740, 1136, 1304 |
| BsmAI | GTCTC | 3 cut(s) | 20, 105, 274 |
| BsmFI | GGGAC | 4 cut(s) | 1076, 1111, 1244, 1279 |
| BsnI | GGCC | 2 cut(s) | 1023, 1191 |
| BsoBI | CYCGRG | 2 cut(s) | 1094, 1262 |
| Bsp1286I | GDGCHC | 6 cut(s) | 208, 347, 424, 524, 601, 807 |
| Bsp1407I | TGTACA | 2 cut(s) | 620, 716 |
| Bsp143I | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 207 |
| BspACI | CCGC | 4 cut(s) | 122, 659, 666, 870 |
| BspANI | GGCC | 2 cut(s) | 1023, 1191 |
| BspCNI | CTCAG | 5 cut(s) | 220, 309, 800, 1127, 1295 |
| BspDI | ATCGAT | 1 cut(s) | 250 |
| BspHI | TCATGA | 1 cut(s) | 130 |
| BspLI | GGNNCC | 2 cut(s) | 1105, 1273 |
| BspMAI | CTGCAG | 2 cut(s) | 1151, 1319 |
| BspMI | ACCTGC | 4 cut(s) | 375, 452, 552, 633 |
| BspPI | GGATC | 1 cut(s) | 113 |
| BspT107I | GGYRCC | 2 cut(s) | 1103, 1271 |
| BsrBI | CCGCTC | 1 cut(s) | 659 |
| BsrDI | GCAATG | 3 cut(s) | 42, 202, 1360 |
| BsrGI | TGTACA | 2 cut(s) | 620, 716 |
| BsrI | ACTGG | 1 cut(s) | 294 |
| BssECI | CCNNGG | 1 cut(s) | 677 |
| BssMI | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| Bst4CI | ACNGT | 4 cut(s) | 14, 678, 896, 979 |
| Bst6I | CTCTTC | 2 cut(s) | 18, 619 |
| BstAPI | GCANNNNNTGC | 5 cut(s) | 993, 1146, 1161, 1314, 1323 |
| BstAUI | TGTACA | 2 cut(s) | 620, 716 |
| BstC8I | GCNNGC | 2 cut(s) | 124, 867 |
| BstDEI | CTNAG | 8 cut(s) | 207, 276, 296, 808, 1043, 1135, 1211, 1303 |
| BstDSI | CCRYGG | 1 cut(s) | 677 |
| BstF5I | GGATG | 2 cut(s) | 755, 818 |
| BstKTI | GATC | 4 cut(s) | 108, 119, 1035, 1203 |
| BstMAI | GTCTC | 3 cut(s) | 20, 105, 274 |
| BstMBI | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 743, 875, 993, 1146, 1161, 1314, 1323 |
| BstNSI | RCATGY | 2 cut(s) | 33, 627 |
| BstSFI | CTRYAG | 3 cut(s) | 975, 1147, 1315 |
| BstSLI | GKGCMC | 1 cut(s) | 807 |
| BstV1I | GCAGC | 5 cut(s) | 956, 1109, 1161, 1164, 1277 |
| Bsu15I | ATCGAT | 1 cut(s) | 250 |
| BsuRI | GGCC | 2 cut(s) | 1023, 1191 |
| BsuTUI | ATCGAT | 1 cut(s) | 250 |
| BtgI | CCRYGG | 1 cut(s) | 677 |
| BtsCI | GGATG | 2 cut(s) | 755, 818 |
| BtsI | GCAGTG | 2 cut(s) | 1086, 1254 |
| BtsIMutI | CAGTG | 6 cut(s) | 304, 805, 901, 984, 1086, 1254 |
| BveI | ACCTGC | 4 cut(s) | 375, 452, 552, 633 |
| Cac8I | GCNNGC | 2 cut(s) | 124, 867 |
| CaiI | CAGNNNCTG | 3 cut(s) | 746, 1122, 1290 |
| CciI | TCATGA | 1 cut(s) | 130 |
| Cfr13I | GGNCC | 2 cut(s) | 1070, 1238 |
| ClaI | ATCGAT | 1 cut(s) | 250 |
| Csp6I | GTAC | 6 cut(s) | 69, 96, 621, 717, 1014, 1182 |
| CviQI | GTAC | 6 cut(s) | 69, 96, 621, 717, 1014, 1182 |
| DdeI | CTNAG | 8 cut(s) | 207, 276, 296, 808, 1043, 1135, 1211, 1303 |
| DpnI | GATC | 4 cut(s) | 107, 118, 1034, 1202 |
| DpnII | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| Eam1104I | CTCTTC | 2 cut(s) | 18, 619 |
| EarI | CTCTTC | 2 cut(s) | 18, 619 |
| Ecl136II | GAGCTC | 4 cut(s) | 345, 422, 522, 599 |
| Eco24I | GRGCYC | 4 cut(s) | 347, 424, 524, 601 |
| Eco47I | GGWCC | 2 cut(s) | 1070, 1238 |
| Eco53kI | GAGCTC | 4 cut(s) | 345, 422, 522, 599 |
| Eco57I | CTGAAG | 9 cut(s) | 363, 440, 540, 621, 723, 765, 894, 982, 1150 |
| Eco88I | CYCGRG | 2 cut(s) | 1094, 1262 |
| EcoICRI | GAGCTC | 4 cut(s) | 345, 422, 522, 599 |
| EcoRI | GAATTC | 1 cut(s) | 307 |
| EcoT38I | GRGCYC | 4 cut(s) | 347, 424, 524, 601 |
| FaqI | GGGAC | 4 cut(s) | 1076, 1111, 1244, 1279 |
| FauI | CCCGC | 2 cut(s) | 115, 652 |
| FbaI | TGATCA | 1 cut(s) | 116 |
| Fnu4HI | GCNGC | 8 cut(s) | 4, 667, 870, 945, 1123, 1150, 1153, 1291 |
| FokI | GGATG | 2 cut(s) | 762, 825 |
| FriOI | GRGCYC | 4 cut(s) | 347, 424, 524, 601 |
| Fsp4HI | GCNGC | 8 cut(s) | 4, 667, 870, 945, 1123, 1150, 1153, 1291 |
| GluI | GCNGC | 8 cut(s) | 4, 667, 870, 945, 1123, 1150, 1153, 1291 |
| GsaI | CCCAGC | 1 cut(s) | 737 |
| HaeIII | GGCC | 2 cut(s) | 1023, 1191 |
| HincII | GTYRAC | 1 cut(s) | 709 |
| HindII | GTYRAC | 1 cut(s) | 709 |
| HindIII | AAGCTT | 2 cut(s) | 1006, 1174 |
| HphI | GGTGA | 3 cut(s) | 303, 666, 722 |
| Hpy166II | GTNNAC | 5 cut(s) | 709, 730, 972, 1086, 1254 |
| Hpy188I | TCNGA | 6 cut(s) | 82, 110, 784, 1136, 1304, 1346 |
| Hpy8I | GTNNAC | 5 cut(s) | 709, 730, 972, 1086, 1254 |
| HpyAV | CCTTC | 8 cut(s) | 244, 324, 387, 464, 564, 645, 695, 896 |
| HpyCH4III | ACNGT | 4 cut(s) | 14, 678, 896, 979 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 743, 875, 993, 1146, 1161, 1314, 1323 |
| HpyF3I | CTNAG | 8 cut(s) | 207, 276, 296, 808, 1043, 1135, 1211, 1303 |
| Ksp22I | TGATCA | 1 cut(s) | 116 |
| Kzo9I | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| LmnI | GCTCC | 3 cut(s) | 11, 218, 862 |
| Lsp1109I | GCAGC | 5 cut(s) | 956, 1109, 1161, 1164, 1277 |
| LweI | GCATC | 9 cut(s) | 182, 389, 466, 489, 566, 983, 1109, 1151, 1277 |
| MaeIII | GTNAC | 1 cut(s) | 145 |
| MalI | GATC | 4 cut(s) | 107, 118, 1034, 1202 |
| MbiI | CCGCTC | 1 cut(s) | 659 |
| MboI | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| MboII | GAAGA | 5 cut(s) | 35, 66, 101, 606, 928 |
| MfeI | CAATTG | 2 cut(s) | 49, 1354 |
| MhlI | GDGCHC | 6 cut(s) | 208, 347, 424, 524, 601, 807 |
| MluCI | AATT | 8 cut(s) | 49, 182, 307, 932, 1141, 1309, 1354, 1367 |
| MlyI | GAGTC | 8 cut(s) | 351, 367, 428, 444, 528, 544, 605, 625 |
| MmeI | TCCRAC | 1 cut(s) | 33 |
| MseI | TTAA | 1 cut(s) | 172 |
| MslI | CAYNNNNRTG | 2 cut(s) | 34, 154 |
| MspA1I | CMGCKG | 2 cut(s) | 122, 872 |
| MunI | CAATTG | 2 cut(s) | 49, 1354 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 743, 875, 993, 1146, 1161, 1314, 1323 |
| NdeII | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| NlaIV | GGNNCC | 2 cut(s) | 1105, 1273 |
| NspI | RCATGY | 2 cut(s) | 33, 627 |
| PagI | TCATGA | 1 cut(s) | 130 |
| PciI | ACATGT | 1 cut(s) | 623 |
| PfeI | GAWTC | 3 cut(s) | 753, 826, 957 |
| PkrI | GCNGC | 8 cut(s) | 5, 668, 871, 946, 1124, 1151, 1154, 1292 |
| PleI | GAGTC | 8 cut(s) | 351, 367, 428, 444, 528, 544, 605, 625 |
| PpsI | GAGTC | 8 cut(s) | 351, 367, 428, 444, 528, 544, 605, 625 |
| PscI | ACATGT | 1 cut(s) | 623 |
| Psp124BI | GAGCTC | 4 cut(s) | 347, 424, 524, 601 |
| PspFI | CCCAGC | 1 cut(s) | 733 |
| PspN4I | GGNNCC | 2 cut(s) | 1105, 1273 |
| PspPI | GGNCC | 2 cut(s) | 1070, 1238 |
| PstI | CTGCAG | 2 cut(s) | 1151, 1319 |
| PstNI | CAGNNNCTG | 3 cut(s) | 746, 1122, 1290 |
| RsaI | GTAC | 6 cut(s) | 70, 97, 622, 718, 1015, 1183 |
| RsaNI | GTAC | 6 cut(s) | 69, 96, 621, 717, 1014, 1182 |
| RseI | CAYNNNNRTG | 2 cut(s) | 34, 154 |
| SacI | GAGCTC | 4 cut(s) | 347, 424, 524, 601 |
| SaqAI | TTAA | 1 cut(s) | 172 |
| SatI | GCNGC | 8 cut(s) | 4, 667, 870, 945, 1123, 1150, 1153, 1291 |
| Sau3AI | GATC | 4 cut(s) | 105, 116, 1032, 1200 |
| Sau96I | GGNCC | 2 cut(s) | 1070, 1238 |
| ScaI | AGTACT | 1 cut(s) | 70 |
| SchI | GAGTC | 8 cut(s) | 351, 367, 428, 444, 528, 544, 605, 625 |
| SduI | GDGCHC | 6 cut(s) | 208, 347, 424, 524, 601, 807 |
| SfaNI | GCATC | 9 cut(s) | 182, 389, 466, 489, 566, 983, 1109, 1151, 1277 |
| SfcI | CTRYAG | 3 cut(s) | 975, 1147, 1315 |
| SinI | GGWCC | 2 cut(s) | 1070, 1238 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 34, 154 |
| SmlI | CTYRAG | 1 cut(s) | 315 |
| SmoI | CTYRAG | 1 cut(s) | 315 |
| Sse9I | AATT | 8 cut(s) | 49, 182, 307, 932, 1141, 1309, 1354, 1367 |
| SsiI | CCGC | 4 cut(s) | 122, 659, 666, 870 |
| SstI | GAGCTC | 4 cut(s) | 347, 424, 524, 601 |
| TaaI | ACNGT | 4 cut(s) | 14, 678, 896, 979 |
| TaqI | TCGA | 4 cut(s) | 21, 226, 250, 960 |
| TasI | AATT | 8 cut(s) | 49, 182, 307, 932, 1141, 1309, 1354, 1367 |
| TatI | WGTACW | 4 cut(s) | 68, 95, 620, 716 |
| TauI | GCSGC | 2 cut(s) | 669, 872 |
| TfiI | GAWTC | 3 cut(s) | 753, 826, 957 |
| Tru1I | TTAA | 1 cut(s) | 172 |
| Tru9I | TTAA | 1 cut(s) | 172 |
| TscAI | CASTG | 6 cut(s) | 304, 812, 901, 984, 1093, 1261 |
| TseI | GCWGC | 6 cut(s) | 3, 944, 1122, 1149, 1152, 1290 |
| TspDTI | ATGAA | 3 cut(s) | 31, 714, 766 |
| TspRI | CASTG | 6 cut(s) | 304, 812, 901, 984, 1093, 1261 |
| VpaK11BI | GGWCC | 2 cut(s) | 1070, 1238 |
| XapI | RAATTY | 2 cut(s) | 307, 1367 |
| XceI | RCATGY | 2 cut(s) | 33, 627 |
| ZrmI | AGTACT | 1 cut(s) | 70 |