NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 270, 321 |
| AccI | GTMKAC | 2 cut(s) | 320, 335 |
| AcsI | RAATTY | 1 cut(s) | 201 |
| AcuI | CTGAAG | 1 cut(s) | 258 |
| AfaI | GTAC | 3 cut(s) | 120, 147, 299 |
| AgsI | TTSAA | 4 cut(s) | 73, 104, 112, 233 |
| AjnI | CCWGG | 1 cut(s) | 465 |
| AluBI | AGCT | 2 cut(s) | 239, 379 |
| AluI | AGCT | 2 cut(s) | 239, 379 |
| Alw21I | GWGCWC | 1 cut(s) | 241 |
| Alw26I | GTCTC | 5 cut(s) | 72, 155, 168, 191, 364 |
| AoxI | GGCC | 1 cut(s) | 487 |
| ApeKI | GCWGC | 4 cut(s) | 29, 53, 56, 379 |
| ApoI | RAATTY | 1 cut(s) | 201 |
| AspLEI | GCGC | 1 cut(s) | 67 |
| AspS9I | GGNCC | 1 cut(s) | 487 |
| AsuHPI | GGTGA | 1 cut(s) | 454 |
| BanII | GRGCYC | 1 cut(s) | 241 |
| BauI | CACGAG | 1 cut(s) | 258 |
| Bbv12I | GWGCWC | 1 cut(s) | 241 |
| BbvI | GCAGC | 4 cut(s) | 16, 40, 43, 366 |
| BccI | CCATC | 1 cut(s) | 80 |
| BciT130I | CCWGG | 1 cut(s) | 467 |
| BclI | TGATCA | 2 cut(s) | 127, 166 |
| BcoDI | GTCTC | 5 cut(s) | 72, 155, 168, 191, 364 |
| BfuAI | ACCTGC | 2 cut(s) | 270, 321 |
| BisI | GCNGC | 4 cut(s) | 30, 54, 57, 380 |
| BlsI | GCNGC | 4 cut(s) | 31, 55, 58, 381 |
| BmcAI | AGTACT | 1 cut(s) | 120 |
| Bme1390I | CCNGG | 1 cut(s) | 467 |
| BmgT120I | GGNCC | 1 cut(s) | 487 |
| BmrFI | CCNGG | 1 cut(s) | 467 |
| BpmI | CTGGAG | 1 cut(s) | 395 |
| BpuEI | CTTGAG | 1 cut(s) | 230 |
| BsaJI | CCNNGG | 1 cut(s) | 465 |
| Bse1I | ACTGG | 1 cut(s) | 378 |
| BseBI | CCWGG | 1 cut(s) | 467 |
| BseDI | CCNNGG | 1 cut(s) | 465 |
| BseMII | CTCAG | 2 cut(s) | 61, 204 |
| BseNI | ACTGG | 1 cut(s) | 378 |
| BseXI | GCAGC | 4 cut(s) | 16, 40, 43, 366 |
| BsgI | GTGCAG | 1 cut(s) | 432 |
| BshFI | GGCC | 1 cut(s) | 489 |
| BsiHKAI | GWGCWC | 1 cut(s) | 241 |
| BsiSI | CCGG | 1 cut(s) | 33 |
| BsmAI | GTCTC | 5 cut(s) | 72, 155, 168, 191, 364 |
| BsmBI | CGTCTC | 1 cut(s) | 72 |
| BsnI | GGCC | 1 cut(s) | 489 |
| Bsp1286I | GDGCHC | 1 cut(s) | 241 |
| Bsp143I | GATC | 3 cut(s) | 127, 166, 412 |
| BspANI | GGCC | 1 cut(s) | 489 |
| BspCNI | CTCAG | 2 cut(s) | 60, 203 |
| BspMI | ACCTGC | 2 cut(s) | 270, 321 |
| BsrI | ACTGG | 1 cut(s) | 378 |
| BssECI | CCNNGG | 1 cut(s) | 465 |
| BssMI | GATC | 3 cut(s) | 127, 166, 412 |
| BssSI | CACGAG | 1 cut(s) | 258 |
| Bst2BI | CACGAG | 1 cut(s) | 258 |
| Bst2UI | CCWGG | 1 cut(s) | 467 |
| Bst4CI | ACNGT | 2 cut(s) | 185, 406 |
| Bst6I | CTCTTC | 2 cut(s) | 68, 259 |
| BstDEI | CTNAG | 3 cut(s) | 47, 190, 326 |
| BstHHI | GCGC | 1 cut(s) | 67 |
| BstKTI | GATC | 3 cut(s) | 130, 169, 415 |
| BstMAI | GTCTC | 5 cut(s) | 72, 155, 168, 191, 364 |
| BstMBI | GATC | 3 cut(s) | 127, 166, 412 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 62 |
| BstNI | CCWGG | 1 cut(s) | 467 |
| BstSCI | CCNGG | 1 cut(s) | 465 |
| BstV1I | GCAGC | 4 cut(s) | 16, 40, 43, 366 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 473 |
| BsuRI | GGCC | 1 cut(s) | 489 |
| BtsI | GCAGTG | 1 cut(s) | 92 |
| BtsIMutI | CAGTG | 4 cut(s) | 92, 198, 411, 499 |
| BveI | ACCTGC | 2 cut(s) | 270, 321 |
| CfoI | GCGC | 1 cut(s) | 67 |
| Cfr13I | GGNCC | 1 cut(s) | 487 |
| CseI | GACGC | 1 cut(s) | 89 |
| Csp6I | GTAC | 3 cut(s) | 119, 146, 298 |
| CviAII | CATG | 3 cut(s) | 242, 491, 496 |
| CviJI | RGCY | 5 cut(s) | 29, 53, 239, 379, 489 |
| CviKI_1 | RGCY | 5 cut(s) | 29, 53, 239, 379, 489 |
| CviQI | GTAC | 3 cut(s) | 119, 146, 298 |
| DdeI | CTNAG | 3 cut(s) | 47, 190, 326 |
| DpnI | GATC | 3 cut(s) | 129, 168, 414 |
| DpnII | GATC | 3 cut(s) | 127, 166, 412 |
| Eam1104I | CTCTTC | 2 cut(s) | 68, 259 |
| EarI | CTCTTC | 2 cut(s) | 68, 259 |
| Ecl136II | GAGCTC | 1 cut(s) | 239 |
| Eco24I | GRGCYC | 1 cut(s) | 241 |
| Eco53kI | GAGCTC | 1 cut(s) | 239 |
| Eco57I | CTGAAG | 1 cut(s) | 258 |
| EcoICRI | GAGCTC | 1 cut(s) | 239 |
| EcoRI | GAATTC | 1 cut(s) | 201 |
| EcoRII | CCWGG | 1 cut(s) | 465 |
| EcoT38I | GRGCYC | 1 cut(s) | 241 |
| Esp3I | CGTCTC | 1 cut(s) | 72 |
| FaeI | CATG | 3 cut(s) | 245, 494, 499 |
| FaiI | YATR | 7 cut(s) | 116, 243, 303, 305, 360, 492, 497 |
| FatI | CATG | 3 cut(s) | 241, 490, 495 |
| FbaI | TGATCA | 2 cut(s) | 127, 166 |
| FblI | GTMKAC | 2 cut(s) | 320, 335 |
| Fnu4HI | GCNGC | 4 cut(s) | 30, 54, 57, 380 |
| FriOI | GRGCYC | 1 cut(s) | 241 |
| Fsp4HI | GCNGC | 4 cut(s) | 30, 54, 57, 380 |
| GlaI | GCGC | 1 cut(s) | 66 |
| GluI | GCNGC | 4 cut(s) | 30, 54, 57, 380 |
| GsuI | CTGGAG | 1 cut(s) | 395 |
| HaeIII | GGCC | 1 cut(s) | 489 |
| HapII | CCGG | 1 cut(s) | 33 |
| HgaI | GACGC | 1 cut(s) | 89 |
| HhaI | GCGC | 1 cut(s) | 67 |
| Hin1II | CATG | 3 cut(s) | 245, 494, 499 |
| Hin6I | GCGC | 1 cut(s) | 65 |
| HinP1I | GCGC | 1 cut(s) | 65 |
| HincII | GTYRAC | 1 cut(s) | 441 |
| HindII | GTYRAC | 1 cut(s) | 441 |
| HinfI | GANTC | 4 cut(s) | 45, 251, 268, 350 |
| HpaII | CCGG | 1 cut(s) | 33 |
| HphI | GGTGA | 1 cut(s) | 454 |
| Hpy166II | GTNNAC | 6 cut(s) | 92, 298, 321, 336, 441, 462 |
| Hpy188I | TCNGA | 3 cut(s) | 132, 310, 355 |
| Hpy188III | TCNNGA | 2 cut(s) | 230, 291 |
| Hpy8I | GTNNAC | 6 cut(s) | 92, 298, 321, 336, 441, 462 |
| HpyAV | CCTTC | 4 cut(s) | 30, 282, 380, 478 |
| HpyCH4III | ACNGT | 2 cut(s) | 185, 406 |
| HpyCH4V | TGCA | 3 cut(s) | 59, 449, 499 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 62 |
| HpyF3I | CTNAG | 3 cut(s) | 47, 190, 326 |
| Hsp92II | CATG | 3 cut(s) | 245, 494, 499 |
| HspAI | GCGC | 1 cut(s) | 65 |
| Ksp22I | TGATCA | 2 cut(s) | 127, 166 |
| Kzo9I | GATC | 3 cut(s) | 127, 166, 412 |
| LmnI | GCTCC | 1 cut(s) | 376 |
| Lsp1109I | GCAGC | 4 cut(s) | 16, 40, 43, 366 |
| MalI | GATC | 3 cut(s) | 129, 168, 414 |
| MboI | GATC | 3 cut(s) | 127, 166, 412 |
| MboII | GAAGA | 4 cut(s) | 85, 116, 151, 246 |
| MfeI | CAATTG | 1 cut(s) | 99 |
| MhlI | GDGCHC | 1 cut(s) | 241 |
| MluCI | AATT | 3 cut(s) | 99, 201, 471 |
| MlyI | GAGTC | 3 cut(s) | 39, 245, 262 |
| MseI | TTAA | 1 cut(s) | 264 |
| MspI | CCGG | 1 cut(s) | 33 |
| MspR9I | CCNGG | 1 cut(s) | 467 |
| MunI | CAATTG | 1 cut(s) | 99 |
| MvaI | CCWGG | 1 cut(s) | 467 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 62 |
| NdeII | GATC | 3 cut(s) | 127, 166, 412 |
| NlaIII | CATG | 3 cut(s) | 245, 494, 499 |
| PfeI | GAWTC | 1 cut(s) | 350 |
| PkrI | GCNGC | 4 cut(s) | 31, 55, 58, 381 |
| PleI | GAGTC | 3 cut(s) | 39, 245, 262 |
| PpsI | GAGTC | 3 cut(s) | 39, 245, 262 |
| Psp124BI | GAGCTC | 1 cut(s) | 241 |
| Psp6I | CCWGG | 1 cut(s) | 465 |
| PspGI | CCWGG | 1 cut(s) | 465 |
| PspPI | GGNCC | 1 cut(s) | 487 |
| RsaI | GTAC | 3 cut(s) | 120, 147, 299 |
| RsaNI | GTAC | 3 cut(s) | 119, 146, 298 |
| SacI | GAGCTC | 1 cut(s) | 241 |
| SaqAI | TTAA | 1 cut(s) | 264 |
| SatI | GCNGC | 4 cut(s) | 30, 54, 57, 380 |
| Sau3AI | GATC | 3 cut(s) | 127, 166, 412 |
| Sau96I | GGNCC | 1 cut(s) | 487 |
| ScaI | AGTACT | 1 cut(s) | 120 |
| SchI | GAGTC | 3 cut(s) | 39, 245, 262 |
| ScrFI | CCNGG | 1 cut(s) | 467 |
| SduI | GDGCHC | 1 cut(s) | 241 |
| SetI | ASST | 6 cut(s) | 241, 284, 335, 381, 420, 471 |
| SmlI | CTYRAG | 1 cut(s) | 209 |
| SmoI | CTYRAG | 1 cut(s) | 209 |
| Sse9I | AATT | 3 cut(s) | 99, 201, 471 |
| SstI | GAGCTC | 1 cut(s) | 241 |
| StyD4I | CCNGG | 1 cut(s) | 465 |
| TaaI | ACNGT | 2 cut(s) | 185, 406 |
| TaqI | TCGA | 1 cut(s) | 411 |
| TasI | AATT | 3 cut(s) | 99, 201, 471 |
| TatI | WGTACW | 3 cut(s) | 118, 145, 297 |
| TfiI | GAWTC | 1 cut(s) | 350 |
| Tru1I | TTAA | 1 cut(s) | 264 |
| Tru9I | TTAA | 1 cut(s) | 264 |
| TscAI | CASTG | 3 cut(s) | 99, 198, 411 |
| TseI | GCWGC | 4 cut(s) | 29, 53, 56, 379 |
| TspDTI | ATGAA | 1 cut(s) | 446 |
| TspRI | CASTG | 3 cut(s) | 99, 198, 411 |
| XapI | RAATTY | 1 cut(s) | 201 |
| XmiI | GTMKAC | 2 cut(s) | 320, 335 |
| ZrmI | AGTACT | 1 cut(s) | 120 |