NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 287 |
| AcsI | RAATTY | 2 cut(s) | 85, 280 |
| AcuI | CTGAAG | 1 cut(s) | 210 |
| AfaI | GTAC | 1 cut(s) | 266 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 18 |
| AgsI | TTSAA | 4 cut(s) | 99, 138, 233, 251 |
| AjnI | CCWGG | 1 cut(s) | 267 |
| AluBI | AGCT | 1 cut(s) | 254 |
| AluI | AGCT | 1 cut(s) | 254 |
| ApeKI | GCWGC | 1 cut(s) | 4 |
| ApoI | RAATTY | 2 cut(s) | 85, 280 |
| AspS9I | GGNCC | 1 cut(s) | 131 |
| AvaII | GGWCC | 1 cut(s) | 131 |
| BciT130I | CCWGG | 1 cut(s) | 269 |
| BfaI | CTAG | 2 cut(s) | 144, 197 |
| BfmI | CTRYAG | 1 cut(s) | 29 |
| BisI | GCNGC | 1 cut(s) | 5 |
| BlsI | GCNGC | 1 cut(s) | 6 |
| Bme1390I | CCNGG | 1 cut(s) | 269 |
| Bme18I | GGWCC | 1 cut(s) | 131 |
| BmgT120I | GGNCC | 1 cut(s) | 131 |
| BmrFI | CCNGG | 1 cut(s) | 269 |
| BmsI | GCATC | 4 cut(s) | 27, 154, 161, 199 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 18 |
| Bse3DI | GCAATG | 1 cut(s) | 78 |
| BseBI | CCWGG | 1 cut(s) | 269 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 18 |
| BseMI | GCAATG | 1 cut(s) | 78 |
| BseMII | CTCAG | 1 cut(s) | 8 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 18 |
| BspACI | CCGC | 1 cut(s) | 287 |
| BspCNI | CTCAG | 1 cut(s) | 9 |
| BspMAI | CTGCAG | 1 cut(s) | 33 |
| BsrDI | GCAATG | 1 cut(s) | 78 |
| Bst2UI | CCWGG | 1 cut(s) | 269 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 28, 37 |
| BstDEI | CTNAG | 1 cut(s) | 17 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 28, 37 |
| BstNI | CCWGG | 1 cut(s) | 269 |
| BstSCI | CCNGG | 1 cut(s) | 267 |
| BstSFI | CTRYAG | 1 cut(s) | 29 |
| BtsIMutI | CAGTG | 1 cut(s) | 216 |
| Cfr13I | GGNCC | 1 cut(s) | 131 |
| Csp6I | GTAC | 1 cut(s) | 265 |
| CviJI | RGCY | 4 cut(s) | 53, 180, 254, 272 |
| CviKI_1 | RGCY | 4 cut(s) | 53, 180, 254, 272 |
| CviQI | GTAC | 1 cut(s) | 265 |
| DdeI | CTNAG | 1 cut(s) | 17 |
| Eco47I | GGWCC | 1 cut(s) | 131 |
| Eco57I | CTGAAG | 1 cut(s) | 210 |
| EcoO109I | RGGNCCY | 1 cut(s) | 131 |
| EcoRI | GAATTC | 1 cut(s) | 280 |
| EcoRII | CCWGG | 1 cut(s) | 267 |
| EcoT22I | ATGCAT | 1 cut(s) | 154 |
| FaiI | YATR | 2 cut(s) | 62, 225 |
| FauI | CCCGC | 1 cut(s) | 280 |
| Fnu4HI | GCNGC | 1 cut(s) | 5 |
| Fsp4HI | GCNGC | 1 cut(s) | 5 |
| FspBI | CTAG | 2 cut(s) | 144, 197 |
| GluI | GCNGC | 1 cut(s) | 5 |
| Hpy188I | TCNGA | 3 cut(s) | 18, 128, 157 |
| HpyAV | CCTTC | 1 cut(s) | 144 |
| HpyCH4V | TGCA | 5 cut(s) | 31, 67, 83, 152, 167 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 28, 37 |
| HpyF3I | CTNAG | 1 cut(s) | 17 |
| LpnPI | CCDG | 3 cut(s) | 41, 254, 281 |
| LweI | GCATC | 4 cut(s) | 27, 154, 161, 199 |
| MaeI | CTAG | 2 cut(s) | 144, 197 |
| MboII | GAAGA | 1 cut(s) | 203 |
| MluCI | AATT | 5 cut(s) | 23, 68, 85, 114, 280 |
| MmeI | TCCRAC | 1 cut(s) | 106 |
| MnlI | CCTC | 2 cut(s) | 12, 122 |
| Mph1103I | ATGCAT | 1 cut(s) | 154 |
| MseI | TTAA | 1 cut(s) | 71 |
| MspA1I | CMGCKG | 1 cut(s) | 287 |
| MspR9I | CCNGG | 1 cut(s) | 269 |
| MvaI | CCWGG | 1 cut(s) | 269 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 28, 37 |
| NsiI | ATGCAT | 1 cut(s) | 154 |
| PkrI | GCNGC | 1 cut(s) | 6 |
| PpuMI | RGGWCCY | 1 cut(s) | 131 |
| Psp5II | RGGWCCY | 1 cut(s) | 131 |
| Psp6I | CCWGG | 1 cut(s) | 267 |
| PspGI | CCWGG | 1 cut(s) | 267 |
| PspPI | GGNCC | 1 cut(s) | 131 |
| PspPPI | RGGWCCY | 1 cut(s) | 131 |
| PstI | CTGCAG | 1 cut(s) | 33 |
| RsaI | GTAC | 1 cut(s) | 266 |
| RsaNI | GTAC | 1 cut(s) | 265 |
| SaqAI | TTAA | 1 cut(s) | 71 |
| SatI | GCNGC | 1 cut(s) | 5 |
| Sau96I | GGNCC | 1 cut(s) | 131 |
| ScrFI | CCNGG | 1 cut(s) | 269 |
| SetI | ASST | 2 cut(s) | 133, 256 |
| SfaNI | GCATC | 4 cut(s) | 27, 154, 161, 199 |
| SfcI | CTRYAG | 1 cut(s) | 29 |
| SinI | GGWCC | 1 cut(s) | 131 |
| Sse9I | AATT | 5 cut(s) | 23, 68, 85, 114, 280 |
| SsiI | CCGC | 1 cut(s) | 287 |
| SspMI | CTAG | 2 cut(s) | 144, 197 |
| StyD4I | CCNGG | 1 cut(s) | 267 |
| TaqI | TCGA | 1 cut(s) | 35 |
| TasI | AATT | 5 cut(s) | 23, 68, 85, 114, 280 |
| Tru1I | TTAA | 1 cut(s) | 71 |
| Tru9I | TTAA | 1 cut(s) | 71 |
| TscAI | CASTG | 1 cut(s) | 223 |
| TseI | GCWGC | 1 cut(s) | 4 |
| TspRI | CASTG | 1 cut(s) | 223 |
| VpaK11BI | GGWCC | 1 cut(s) | 131 |
| XapI | RAATTY | 2 cut(s) | 85, 280 |
| XspI | CTAG | 2 cut(s) | 144, 197 |
| Zsp2I | ATGCAT | 1 cut(s) | 154 |