NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 55, 578 |
| AccB1I | GGYRCC | 2 cut(s) | 784, 1069 |
| AccI | GTMKAC | 2 cut(s) | 151, 825 |
| AciI | CCGC | 2 cut(s) | 644, 1367 |
| AclWI | GGATC | 2 cut(s) | 271, 401 |
| AcsI | RAATTY | 3 cut(s) | 510, 1165, 1360 |
| AcuI | CTGAAG | 6 cut(s) | 566, 701, 745, 857, 945, 1290 |
| AfaI | GTAC | 5 cut(s) | 228, 255, 696, 978, 1346 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 661, 718, 1102 |
| AgsI | TTSAA | 9 cut(s) | 73, 212, 220, 233, 542, 920, 968, 1218, 1313 |
| AluBI | AGCT | 8 cut(s) | 424, 548, 628, 715, 971, 1013, 1091, 1352 |
| AluI | AGCT | 8 cut(s) | 424, 548, 628, 715, 971, 1013, 1091, 1352 |
| Alw21I | GWGCWC | 3 cut(s) | 366, 550, 610 |
| Alw26I | GTCTC | 3 cut(s) | 178, 263, 477 |
| Alw44I | GTGCAC | 1 cut(s) | 606 |
| AlwI | GGATC | 2 cut(s) | 271, 401 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 724 |
| Ama87I | CYCGRG | 1 cut(s) | 1060 |
| AoxI | GGCC | 1 cut(s) | 984 |
| ApaLI | GTGCAC | 1 cut(s) | 606 |
| ApeKI | GCWGC | 5 cut(s) | 133, 161, 628, 832, 1088 |
| ApoI | RAATTY | 3 cut(s) | 510, 1165, 1360 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 869 |
| AspLEI | GCGC | 1 cut(s) | 175 |
| AspS9I | GGNCC | 2 cut(s) | 1036, 1211 |
| AsuHPI | GGTGA | 3 cut(s) | 644, 700, 1340 |
| AvaI | CYCGRG | 1 cut(s) | 1060 |
| AvaII | GGWCC | 2 cut(s) | 1036, 1211 |
| BaeGI | GKGCMC | 2 cut(s) | 610, 787 |
| BanI | GGYRCC | 2 cut(s) | 784, 1069 |
| BanII | GRGCYC | 1 cut(s) | 550 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 960, 992 |
| BauI | CACGAG | 1 cut(s) | 567 |
| Bbv12I | GWGCWC | 3 cut(s) | 366, 550, 610 |
| BbvI | GCAGC | 5 cut(s) | 120, 148, 615, 819, 1100 |
| BccI | CCATC | 3 cut(s) | 188, 418, 821 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 22, 56 |
| BclI | TGATCA | 1 cut(s) | 274 |
| BcoDI | GTCTC | 3 cut(s) | 178, 263, 477 |
| BfaI | CTAG | 3 cut(s) | 1224, 1277, 1320 |
| BfmI | CTRYAG | 2 cut(s) | 938, 1113 |
| BfuAI | ACCTGC | 2 cut(s) | 55, 578 |
| BisI | GCNGC | 6 cut(s) | 134, 162, 629, 645, 833, 1089 |
| BlpI | GCTNAGC | 1 cut(s) | 365 |
| BlsI | GCNGC | 6 cut(s) | 135, 163, 630, 646, 834, 1090 |
| BmcAI | AGTACT | 2 cut(s) | 228, 1346 |
| Bme18I | GGWCC | 2 cut(s) | 1036, 1211 |
| BmeT110I | CYCGRG | 1 cut(s) | 1060 |
| BmgT120I | GGNCC | 2 cut(s) | 1036, 1211 |
| BmiI | GGNNCC | 2 cut(s) | 786, 1071 |
| BmsI | GCATC | 5 cut(s) | 340, 946, 1075, 1241, 1279 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 382, 414 |
| BpmI | CTGGAG | 2 cut(s) | 409, 644 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 365 |
| BpuEI | CTTGAG | 1 cut(s) | 539 |
| Bsa29I | ATCGAT | 1 cut(s) | 453 |
| BsaJI | CCNNGG | 2 cut(s) | 655, 788 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 661, 718, 1102 |
| Bse1I | ACTGG | 3 cut(s) | 392, 497, 627 |
| Bse3DI | GCAATG | 3 cut(s) | 200, 360, 1158 |
| BseCI | ATCGAT | 1 cut(s) | 453 |
| BseDI | CCNNGG | 2 cut(s) | 655, 788 |
| BseGI | GGATG | 2 cut(s) | 733, 798 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 661, 718, 1102 |
| BseMI | GCAATG | 3 cut(s) | 200, 360, 1158 |
| BseMII | CTCAG | 4 cut(s) | 169, 379, 513, 1092 |
| BseNI | ACTGG | 3 cut(s) | 392, 497, 627 |
| BseRI | GAGGAG | 1 cut(s) | 389 |
| BseSI | GKGCMC | 2 cut(s) | 610, 787 |
| BseXI | GCAGC | 5 cut(s) | 120, 148, 615, 819, 1100 |
| BseYI | CCCAGC | 1 cut(s) | 711 |
| BshFI | GGCC | 1 cut(s) | 986 |
| BshNI | GGYRCC | 2 cut(s) | 784, 1069 |
| BshVI | ATCGAT | 1 cut(s) | 453 |
| BsiHKAI | GWGCWC | 3 cut(s) | 366, 550, 610 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1060 |
| BsiSI | CCGG | 1 cut(s) | 137 |
| BslFI | GGGAC | 2 cut(s) | 1042, 1077 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 661, 718, 1102 |
| BsmAI | GTCTC | 3 cut(s) | 178, 263, 477 |
| BsmFI | GGGAC | 2 cut(s) | 1042, 1077 |
| BsmI | GAATGC | 1 cut(s) | 869 |
| BsnI | GGCC | 1 cut(s) | 986 |
| BsoBI | CYCGRG | 1 cut(s) | 1060 |
| Bsp1286I | GDGCHC | 4 cut(s) | 366, 550, 610, 787 |
| Bsp1407I | TGTACA | 1 cut(s) | 694 |
| Bsp143I | GATC | 4 cut(s) | 263, 274, 406, 995 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 365 |
| BspACI | CCGC | 2 cut(s) | 644, 1367 |
| BspANI | GGCC | 1 cut(s) | 986 |
| BspCNI | CTCAG | 4 cut(s) | 168, 378, 512, 1093 |
| BspDI | ATCGAT | 1 cut(s) | 453 |
| BspHI | TCATGA | 1 cut(s) | 288 |
| BspLI | GGNNCC | 2 cut(s) | 786, 1071 |
| BspMAI | CTGCAG | 1 cut(s) | 1117 |
| BspMI | ACCTGC | 2 cut(s) | 55, 578 |
| BspPI | GGATC | 2 cut(s) | 271, 401 |
| BspT107I | GGYRCC | 2 cut(s) | 784, 1069 |
| BsrDI | GCAATG | 3 cut(s) | 200, 360, 1158 |
| BsrGI | TGTACA | 1 cut(s) | 694 |
| BsrI | ACTGG | 3 cut(s) | 392, 497, 627 |
| BssECI | CCNNGG | 2 cut(s) | 655, 788 |
| BssMI | GATC | 4 cut(s) | 263, 274, 406, 995 |
| BssNAI | GTATAC | 1 cut(s) | 826 |
| BssSI | CACGAG | 1 cut(s) | 567 |
| Bst1107I | GTATAC | 1 cut(s) | 826 |
| Bst2BI | CACGAG | 1 cut(s) | 567 |
| Bst4CI | ACNGT | 4 cut(s) | 65, 656, 859, 942 |
| Bst6I | CTCTTC | 2 cut(s) | 176, 568 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 956, 1112, 1121 |
| BstAUI | TGTACA | 1 cut(s) | 694 |
| BstC8I | GCNNGC | 2 cut(s) | 282, 606 |
| BstDEI | CTNAG | 7 cut(s) | 155, 365, 479, 499, 1009, 1101, 1348 |
| BstDSI | CCRYGG | 1 cut(s) | 655 |
| BstF5I | GGATG | 2 cut(s) | 733, 798 |
| BstHHI | GCGC | 1 cut(s) | 175 |
| BstKTI | GATC | 4 cut(s) | 266, 277, 409, 998 |
| BstMAI | GTCTC | 3 cut(s) | 178, 263, 477 |
| BstMBI | GATC | 4 cut(s) | 263, 274, 406, 995 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 56, 170, 721, 838, 956, 1010, 1112, 1121 |
| BstNSI | RCATGY | 1 cut(s) | 191 |
| BstSFI | CTRYAG | 2 cut(s) | 938, 1113 |
| BstSLI | GKGCMC | 2 cut(s) | 610, 787 |
| BstV1I | GCAGC | 5 cut(s) | 120, 148, 615, 819, 1100 |
| BstZ17I | GTATAC | 1 cut(s) | 826 |
| Bsu15I | ATCGAT | 1 cut(s) | 453 |
| BsuRI | GGCC | 1 cut(s) | 986 |
| BsuTUI | ATCGAT | 1 cut(s) | 453 |
| BtgI | CCRYGG | 1 cut(s) | 655 |
| BtsCI | GGATG | 2 cut(s) | 733, 798 |
| BtsI | GCAGTG | 1 cut(s) | 1052 |
| BtsIMutI | CAGTG | 6 cut(s) | 61, 507, 864, 947, 1052, 1296 |
| BveI | ACCTGC | 2 cut(s) | 55, 578 |
| Cac8I | GCNNGC | 2 cut(s) | 282, 606 |
| CaiI | CAGNNNCTG | 1 cut(s) | 724 |
| CciI | TCATGA | 1 cut(s) | 288 |
| CfoI | GCGC | 1 cut(s) | 175 |
| Cfr13I | GGNCC | 2 cut(s) | 1036, 1211 |
| ClaI | ATCGAT | 1 cut(s) | 453 |
| CseI | GACGC | 1 cut(s) | 1164 |
| Csp6I | GTAC | 5 cut(s) | 227, 254, 695, 977, 1345 |
| CviAII | CATG | 6 cut(s) | 188, 289, 370, 475, 551, 982 |
| CviQI | GTAC | 5 cut(s) | 227, 254, 695, 977, 1345 |
| DdeI | CTNAG | 7 cut(s) | 155, 365, 479, 499, 1009, 1101, 1348 |
| DpnI | GATC | 4 cut(s) | 265, 276, 408, 997 |
| DpnII | GATC | 4 cut(s) | 263, 274, 406, 995 |
| Eam1104I | CTCTTC | 2 cut(s) | 176, 568 |
| EarI | CTCTTC | 2 cut(s) | 176, 568 |
| Ecl136II | GAGCTC | 1 cut(s) | 548 |
| Eco24I | GRGCYC | 1 cut(s) | 550 |
| Eco32I | GATATC | 1 cut(s) | 20 |
| Eco47I | GGWCC | 2 cut(s) | 1036, 1211 |
| Eco53kI | GAGCTC | 1 cut(s) | 548 |
| Eco57I | CTGAAG | 6 cut(s) | 566, 701, 745, 857, 945, 1290 |
| Eco88I | CYCGRG | 1 cut(s) | 1060 |
| EcoICRI | GAGCTC | 1 cut(s) | 548 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1211 |
| EcoRI | GAATTC | 2 cut(s) | 510, 1360 |
| EcoRV | GATATC | 1 cut(s) | 20 |
| EcoT22I | ATGCAT | 1 cut(s) | 1234 |
| EcoT38I | GRGCYC | 1 cut(s) | 550 |
| FaeI | CATG | 6 cut(s) | 191, 292, 373, 478, 554, 985 |
| FaqI | GGGAC | 2 cut(s) | 1042, 1077 |
| FatI | CATG | 6 cut(s) | 187, 288, 369, 474, 550, 981 |
| FauI | CCCGC | 1 cut(s) | 1360 |
| FbaI | TGATCA | 1 cut(s) | 274 |
| FblI | GTMKAC | 2 cut(s) | 151, 825 |
| Fnu4HI | GCNGC | 6 cut(s) | 134, 162, 629, 645, 833, 1089 |
| FokI | GGATG | 2 cut(s) | 740, 805 |
| FriOI | GRGCYC | 1 cut(s) | 550 |
| Fsp4HI | GCNGC | 6 cut(s) | 134, 162, 629, 645, 833, 1089 |
| FspBI | CTAG | 3 cut(s) | 1224, 1277, 1320 |
| GlaI | GCGC | 1 cut(s) | 174 |
| GluI | GCNGC | 6 cut(s) | 134, 162, 629, 645, 833, 1089 |
| GsaI | CCCAGC | 1 cut(s) | 715 |
| GsuI | CTGGAG | 2 cut(s) | 409, 644 |
| HaeIII | GGCC | 1 cut(s) | 986 |
| HapII | CCGG | 1 cut(s) | 137 |
| HgaI | GACGC | 1 cut(s) | 1164 |
| HhaI | GCGC | 1 cut(s) | 175 |
| Hin1II | CATG | 6 cut(s) | 191, 292, 373, 478, 554, 985 |
| Hin6I | GCGC | 1 cut(s) | 173 |
| HinP1I | GCGC | 1 cut(s) | 173 |
| HincII | GTYRAC | 1 cut(s) | 687 |
| HindII | GTYRAC | 1 cut(s) | 687 |
| HindIII | AAGCTT | 1 cut(s) | 969 |
| HinfI | GANTC | 6 cut(s) | 153, 560, 576, 731, 920, 1244 |
| HpaII | CCGG | 1 cut(s) | 137 |
| HphI | GGTGA | 3 cut(s) | 644, 700, 1340 |
| Hpy166II | GTNNAC | 7 cut(s) | 152, 608, 687, 708, 826, 935, 1052 |
| Hpy188I | TCNGA | 8 cut(s) | 87, 240, 268, 764, 1102, 1144, 1237, 1249 |
| Hpy188III | TCNNGA | 6 cut(s) | 289, 539, 599, 803, 917, 1062 |
| Hpy8I | GTNNAC | 7 cut(s) | 152, 608, 687, 708, 826, 935, 1052 |
| HpyAV | CCTTC | 9 cut(s) | 134, 138, 447, 527, 590, 629, 673, 859, 1224 |
| HpyCH4III | ACNGT | 4 cut(s) | 65, 656, 859, 942 |
| HpyCH4IV | ACGT | 1 cut(s) | 1220 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 56, 170, 721, 838, 956, 1010, 1112, 1121 |
| HpyF3I | CTNAG | 7 cut(s) | 155, 365, 479, 499, 1009, 1101, 1348 |
| HpySE526I | ACGT | 1 cut(s) | 1220 |
| Hsp92II | CATG | 6 cut(s) | 191, 292, 373, 478, 554, 985 |
| HspAI | GCGC | 1 cut(s) | 173 |
| Ksp22I | TGATCA | 1 cut(s) | 274 |
| Kzo9I | GATC | 4 cut(s) | 263, 274, 406, 995 |
| LmnI | GCTCC | 2 cut(s) | 169, 625 |
| Lsp1109I | GCAGC | 5 cut(s) | 120, 148, 615, 819, 1100 |
| LweI | GCATC | 5 cut(s) | 340, 946, 1075, 1241, 1279 |
| MaeI | CTAG | 3 cut(s) | 1224, 1277, 1320 |
| MaeII | ACGT | 1 cut(s) | 1220 |
| MaeIII | GTNAC | 3 cut(s) | 303, 383, 1315 |
| MalI | GATC | 4 cut(s) | 265, 276, 408, 997 |
| MboI | GATC | 4 cut(s) | 263, 274, 406, 995 |
| MboII | GAAGA | 7 cut(s) | 48, 193, 224, 259, 555, 891, 1283 |
| MfeI | CAATTG | 2 cut(s) | 207, 1152 |
| MhlI | GDGCHC | 4 cut(s) | 366, 550, 610, 787 |
| MluCI | AATT | 8 cut(s) | 207, 340, 510, 895, 1107, 1152, 1165, 1360 |
| MlyI | GAGTC | 3 cut(s) | 147, 554, 570 |
| MmeI | TCCRAC | 2 cut(s) | 191, 1186 |
| MnlI | CCTC | 9 cut(s) | 367, 370, 384, 783, 841, 918, 1019, 1096, 1202 |
| Mph1103I | ATGCAT | 1 cut(s) | 1234 |
| MroXI | GAANNNNTTC | 1 cut(s) | 869 |
| MseI | TTAA | 2 cut(s) | 330, 420 |
| MslI | CAYNNNNRTG | 2 cut(s) | 192, 312 |
| MspA1I | CMGCKG | 1 cut(s) | 1367 |
| MspI | CCGG | 1 cut(s) | 137 |
| MunI | CAATTG | 2 cut(s) | 207, 1152 |
| Mva1269I | GAATGC | 1 cut(s) | 869 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 56, 170, 721, 838, 956, 1010, 1112, 1121 |
| NdeII | GATC | 4 cut(s) | 263, 274, 406, 995 |
| NlaIII | CATG | 6 cut(s) | 191, 292, 373, 478, 554, 985 |
| NlaIV | GGNNCC | 2 cut(s) | 786, 1071 |
| NsiI | ATGCAT | 1 cut(s) | 1234 |
| NspI | RCATGY | 1 cut(s) | 191 |
| PagI | TCATGA | 1 cut(s) | 288 |
| PctI | GAATGC | 1 cut(s) | 869 |
| PdmI | GAANNNNTTC | 1 cut(s) | 869 |
| PfeI | GAWTC | 3 cut(s) | 731, 920, 1244 |
| PkrI | GCNGC | 6 cut(s) | 135, 163, 630, 646, 834, 1090 |
| PleI | GAGTC | 3 cut(s) | 147, 554, 570 |
| PpsI | GAGTC | 3 cut(s) | 147, 554, 570 |
| PpuMI | RGGWCCY | 1 cut(s) | 1211 |
| Psp124BI | GAGCTC | 1 cut(s) | 550 |
| Psp5II | RGGWCCY | 1 cut(s) | 1211 |
| PspFI | CCCAGC | 1 cut(s) | 711 |
| PspN4I | GGNNCC | 2 cut(s) | 786, 1071 |
| PspPI | GGNCC | 2 cut(s) | 1036, 1211 |
| PspPPI | RGGWCCY | 1 cut(s) | 1211 |
| PstI | CTGCAG | 1 cut(s) | 1117 |
| PstNI | CAGNNNCTG | 1 cut(s) | 724 |
| RsaI | GTAC | 5 cut(s) | 228, 255, 696, 978, 1346 |
| RsaNI | GTAC | 5 cut(s) | 227, 254, 695, 977, 1345 |
| RseI | CAYNNNNRTG | 2 cut(s) | 192, 312 |
| SacI | GAGCTC | 1 cut(s) | 550 |
| SaqAI | TTAA | 2 cut(s) | 330, 420 |
| SatI | GCNGC | 6 cut(s) | 134, 162, 629, 645, 833, 1089 |
| Sau3AI | GATC | 4 cut(s) | 263, 274, 406, 995 |
| Sau96I | GGNCC | 2 cut(s) | 1036, 1211 |
| ScaI | AGTACT | 2 cut(s) | 228, 1346 |
| SchI | GAGTC | 3 cut(s) | 147, 554, 570 |
| SduI | GDGCHC | 4 cut(s) | 366, 550, 610, 787 |
| SfaNI | GCATC | 5 cut(s) | 340, 946, 1075, 1241, 1279 |
| SfcI | CTRYAG | 2 cut(s) | 938, 1113 |
| SinI | GGWCC | 2 cut(s) | 1036, 1211 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 192, 312 |
| SmlI | CTYRAG | 1 cut(s) | 518 |
| SmoI | CTYRAG | 1 cut(s) | 518 |
| Sse9I | AATT | 8 cut(s) | 207, 340, 510, 895, 1107, 1152, 1165, 1360 |
| SsiI | CCGC | 2 cut(s) | 644, 1367 |
| SspMI | CTAG | 3 cut(s) | 1224, 1277, 1320 |
| SstI | GAGCTC | 1 cut(s) | 550 |
| TaaI | ACNGT | 4 cut(s) | 65, 656, 859, 942 |
| TaiI | ACGT | 1 cut(s) | 1223 |
| TaqI | TCGA | 6 cut(s) | 93, 179, 429, 453, 873, 923 |
| TasI | AATT | 8 cut(s) | 207, 340, 510, 895, 1107, 1152, 1165, 1360 |
| TatI | WGTACW | 4 cut(s) | 226, 253, 694, 1344 |
| TauI | GCSGC | 1 cut(s) | 647 |
| TfiI | GAWTC | 3 cut(s) | 731, 920, 1244 |
| Tru1I | TTAA | 2 cut(s) | 330, 420 |
| Tru9I | TTAA | 2 cut(s) | 330, 420 |
| TscAI | CASTG | 6 cut(s) | 68, 507, 864, 947, 1059, 1303 |
| TseI | GCWGC | 5 cut(s) | 133, 161, 628, 832, 1088 |
| TspDTI | ATGAA | 4 cut(s) | 189, 463, 692, 744 |
| TspRI | CASTG | 6 cut(s) | 68, 507, 864, 947, 1059, 1303 |
| VneI | GTGCAC | 1 cut(s) | 606 |
| VpaK11BI | GGWCC | 2 cut(s) | 1036, 1211 |
| XapI | RAATTY | 3 cut(s) | 510, 1165, 1360 |
| XceI | RCATGY | 1 cut(s) | 191 |
| XmiI | GTMKAC | 2 cut(s) | 151, 825 |
| XmnI | GAANNNNTTC | 1 cut(s) | 869 |
| XspI | CTAG | 3 cut(s) | 1224, 1277, 1320 |
| ZrmI | AGTACT | 2 cut(s) | 228, 1346 |
| Zsp2I | ATGCAT | 1 cut(s) | 1234 |