NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 521 |
| AccB1I | GGYRCC | 2 cut(s) | 243, 997 |
| AccI | GTMKAC | 1 cut(s) | 768 |
| AciI | CCGC | 2 cut(s) | 133, 587 |
| AclWI | GGATC | 2 cut(s) | 124, 388 |
| AcsI | RAATTY | 3 cut(s) | 278, 453, 1093 |
| AcuI | CTGAAG | 6 cut(s) | 509, 644, 688, 800, 873, 1218 |
| AfaI | GTAC | 4 cut(s) | 81, 108, 906, 1274 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 661, 1030 |
| AgsI | TTSAA | 7 cut(s) | 65, 73, 485, 848, 896, 1146, 1241 |
| AluBI | AGCT | 8 cut(s) | 28, 319, 411, 491, 571, 658, 899, 941 |
| AluI | AGCT | 8 cut(s) | 28, 319, 411, 491, 571, 658, 899, 941 |
| Alw21I | GWGCWC | 3 cut(s) | 219, 493, 553 |
| Alw26I | GTCTC | 3 cut(s) | 31, 116, 330 |
| Alw44I | GTGCAC | 1 cut(s) | 549 |
| AlwI | GGATC | 2 cut(s) | 124, 388 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 125, 322, 1016 |
| Ama87I | CYCGRG | 1 cut(s) | 988 |
| AoxI | GGCC | 1 cut(s) | 912 |
| ApaLI | GTGCAC | 1 cut(s) | 549 |
| ApeKI | GCWGC | 5 cut(s) | 14, 316, 571, 775, 1016 |
| ApoI | RAATTY | 3 cut(s) | 278, 453, 1093 |
| AspS9I | GGNCC | 1 cut(s) | 964 |
| AsuHPI | GGTGA | 3 cut(s) | 449, 643, 1268 |
| AvaI | CYCGRG | 1 cut(s) | 988 |
| AvaII | GGWCC | 1 cut(s) | 964 |
| BaeGI | GKGCMC | 2 cut(s) | 553, 730 |
| BanI | GGYRCC | 2 cut(s) | 243, 997 |
| BanII | GRGCYC | 1 cut(s) | 493 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 888, 920 |
| BauI | CACGAG | 1 cut(s) | 510 |
| Bbv12I | GWGCWC | 3 cut(s) | 219, 493, 553 |
| BbvI | GCAGC | 4 cut(s) | 328, 558, 762, 1003 |
| BccI | CCATC | 3 cut(s) | 41, 405, 764 |
| BclI | TGATCA | 2 cut(s) | 127, 693 |
| BcoDI | GTCTC | 3 cut(s) | 31, 116, 330 |
| BfaI | CTAG | 2 cut(s) | 1152, 1205 |
| BfmI | CTRYAG | 2 cut(s) | 866, 1041 |
| BfuAI | ACCTGC | 1 cut(s) | 521 |
| BisI | GCNGC | 6 cut(s) | 15, 317, 572, 588, 776, 1017 |
| BlpI | GCTNAGC | 1 cut(s) | 218 |
| BlsI | GCNGC | 6 cut(s) | 16, 318, 573, 589, 777, 1018 |
| BmcAI | AGTACT | 2 cut(s) | 81, 1274 |
| Bme18I | GGWCC | 1 cut(s) | 964 |
| BmeT110I | CYCGRG | 1 cut(s) | 988 |
| BmgT120I | GGNCC | 1 cut(s) | 964 |
| BmiI | GGNNCC | 2 cut(s) | 245, 999 |
| BmrI | ACTGGG | 1 cut(s) | 740 |
| BmsI | GCATC | 6 cut(s) | 193, 850, 874, 1003, 1169, 1207 |
| BmuI | ACTGGG | 1 cut(s) | 740 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 367, 399 |
| BpmI | CTGGAG | 1 cut(s) | 587 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 218 |
| BpuEI | CTTGAG | 1 cut(s) | 482 |
| Bsa29I | ATCGAT | 1 cut(s) | 440 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 303, 333 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 661, 1030 |
| Bse1I | ACTGG | 3 cut(s) | 245, 570, 735 |
| Bse3DI | GCAATG | 3 cut(s) | 53, 213, 1086 |
| BseCI | ATCGAT | 1 cut(s) | 440 |
| BseGI | GGATG | 2 cut(s) | 676, 741 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 661, 1030 |
| BseMI | GCAATG | 3 cut(s) | 53, 213, 1086 |
| BseMII | CTCAG | 3 cut(s) | 15, 232, 1020 |
| BseNI | ACTGG | 3 cut(s) | 245, 570, 735 |
| BseRI | GAGGAG | 1 cut(s) | 242 |
| BseSI | GKGCMC | 2 cut(s) | 553, 730 |
| BseXI | GCAGC | 4 cut(s) | 328, 558, 762, 1003 |
| BseYI | CCCAGC | 1 cut(s) | 654 |
| BsgI | GTGCAG | 1 cut(s) | 621 |
| BshFI | GGCC | 1 cut(s) | 914 |
| BshNI | GGYRCC | 2 cut(s) | 243, 997 |
| BshVI | ATCGAT | 1 cut(s) | 440 |
| BsiHKAI | GWGCWC | 3 cut(s) | 219, 493, 553 |
| BsiHKCI | CYCGRG | 1 cut(s) | 988 |
| BslFI | GGGAC | 2 cut(s) | 970, 1005 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 661, 1030 |
| BsmAI | GTCTC | 3 cut(s) | 31, 116, 330 |
| BsmFI | GGGAC | 2 cut(s) | 970, 1005 |
| BsnI | GGCC | 1 cut(s) | 914 |
| BsoBI | CYCGRG | 1 cut(s) | 988 |
| Bsp1286I | GDGCHC | 4 cut(s) | 219, 493, 553, 730 |
| Bsp143I | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 218 |
| BspACI | CCGC | 2 cut(s) | 133, 587 |
| BspANI | GGCC | 1 cut(s) | 914 |
| BspCNI | CTCAG | 3 cut(s) | 16, 231, 1021 |
| BspDI | ATCGAT | 1 cut(s) | 440 |
| BspHI | TCATGA | 1 cut(s) | 141 |
| BspLI | GGNNCC | 2 cut(s) | 245, 999 |
| BspMAI | CTGCAG | 1 cut(s) | 1045 |
| BspMI | ACCTGC | 1 cut(s) | 521 |
| BspPI | GGATC | 2 cut(s) | 124, 388 |
| BspT107I | GGYRCC | 2 cut(s) | 243, 997 |
| BsrDI | GCAATG | 3 cut(s) | 53, 213, 1086 |
| BsrI | ACTGG | 3 cut(s) | 245, 570, 735 |
| BssMI | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| BssNAI | GTATAC | 1 cut(s) | 769 |
| BssSI | CACGAG | 1 cut(s) | 510 |
| Bst1107I | GTATAC | 1 cut(s) | 769 |
| Bst2BI | CACGAG | 1 cut(s) | 510 |
| Bst4CI | ACNGT | 4 cut(s) | 599, 625, 802, 870 |
| Bst6I | CTCTTC | 3 cut(s) | 29, 95, 511 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 884, 1040, 1049 |
| BstC8I | GCNNGC | 2 cut(s) | 135, 549 |
| BstDEI | CTNAG | 5 cut(s) | 24, 218, 937, 1029, 1281 |
| BstF5I | GGATG | 2 cut(s) | 676, 741 |
| BstKTI | GATC | 5 cut(s) | 119, 130, 396, 696, 926 |
| BstMAI | GTCTC | 3 cut(s) | 31, 116, 330 |
| BstMBI | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 781, 884, 938, 1040, 1049 |
| BstNSI | RCATGY | 1 cut(s) | 44 |
| BstSFI | CTRYAG | 2 cut(s) | 866, 1041 |
| BstSLI | GKGCMC | 2 cut(s) | 553, 730 |
| BstV1I | GCAGC | 4 cut(s) | 328, 558, 762, 1003 |
| BstZ17I | GTATAC | 1 cut(s) | 769 |
| Bsu15I | ATCGAT | 1 cut(s) | 440 |
| BsuRI | GGCC | 1 cut(s) | 914 |
| BsuTUI | ATCGAT | 1 cut(s) | 440 |
| BtsCI | GGATG | 2 cut(s) | 676, 741 |
| BtsIMutI | CAGTG | 7 cut(s) | 604, 624, 728, 807, 875, 1113, 1224 |
| BveI | ACCTGC | 1 cut(s) | 521 |
| Cac8I | GCNNGC | 2 cut(s) | 135, 549 |
| CaiI | CAGNNNCTG | 3 cut(s) | 125, 322, 1016 |
| CciI | TCATGA | 1 cut(s) | 141 |
| Cfr13I | GGNCC | 1 cut(s) | 964 |
| ClaI | ATCGAT | 1 cut(s) | 440 |
| CseI | GACGC | 1 cut(s) | 1092 |
| Csp6I | GTAC | 4 cut(s) | 80, 107, 905, 1273 |
| CviAII | CATG | 7 cut(s) | 41, 142, 223, 303, 494, 697, 910 |
| CviQI | GTAC | 4 cut(s) | 80, 107, 905, 1273 |
| DdeI | CTNAG | 5 cut(s) | 24, 218, 937, 1029, 1281 |
| DpnI | GATC | 5 cut(s) | 118, 129, 395, 695, 925 |
| DpnII | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| Eam1104I | CTCTTC | 3 cut(s) | 29, 95, 511 |
| EarI | CTCTTC | 3 cut(s) | 29, 95, 511 |
| Ecl136II | GAGCTC | 1 cut(s) | 491 |
| Eco24I | GRGCYC | 1 cut(s) | 493 |
| Eco47I | GGWCC | 1 cut(s) | 964 |
| Eco53kI | GAGCTC | 1 cut(s) | 491 |
| Eco57I | CTGAAG | 6 cut(s) | 509, 644, 688, 800, 873, 1218 |
| Eco88I | CYCGRG | 1 cut(s) | 988 |
| EcoICRI | GAGCTC | 1 cut(s) | 491 |
| EcoRI | GAATTC | 1 cut(s) | 453 |
| EcoT22I | ATGCAT | 1 cut(s) | 1162 |
| EcoT38I | GRGCYC | 1 cut(s) | 493 |
| FaeI | CATG | 7 cut(s) | 44, 145, 226, 306, 497, 700, 913 |
| FaqI | GGGAC | 2 cut(s) | 970, 1005 |
| FatI | CATG | 7 cut(s) | 40, 141, 222, 302, 493, 696, 909 |
| FauI | CCCGC | 1 cut(s) | 126 |
| FauNDI | CATATG | 1 cut(s) | 273 |
| FbaI | TGATCA | 2 cut(s) | 127, 693 |
| FblI | GTMKAC | 1 cut(s) | 768 |
| Fnu4HI | GCNGC | 6 cut(s) | 15, 317, 572, 588, 776, 1017 |
| FokI | GGATG | 2 cut(s) | 683, 748 |
| FriOI | GRGCYC | 1 cut(s) | 493 |
| Fsp4HI | GCNGC | 6 cut(s) | 15, 317, 572, 588, 776, 1017 |
| FspBI | CTAG | 2 cut(s) | 1152, 1205 |
| GluI | GCNGC | 6 cut(s) | 15, 317, 572, 588, 776, 1017 |
| GsaI | CCCAGC | 1 cut(s) | 658 |
| GsuI | CTGGAG | 1 cut(s) | 587 |
| HaeIII | GGCC | 1 cut(s) | 914 |
| HgaI | GACGC | 1 cut(s) | 1092 |
| Hin1II | CATG | 7 cut(s) | 44, 145, 226, 306, 497, 700, 913 |
| HincII | GTYRAC | 2 cut(s) | 630, 980 |
| HindII | GTYRAC | 2 cut(s) | 630, 980 |
| HindIII | AAGCTT | 1 cut(s) | 897 |
| HinfI | GANTC | 5 cut(s) | 503, 519, 674, 848, 1172 |
| HphI | GGTGA | 3 cut(s) | 449, 643, 1268 |
| Hpy166II | GTNNAC | 6 cut(s) | 551, 630, 651, 769, 863, 980 |
| Hpy188I | TCNGA | 7 cut(s) | 93, 121, 707, 1030, 1072, 1165, 1177 |
| Hpy188III | TCNNGA | 6 cut(s) | 142, 482, 542, 746, 845, 990 |
| Hpy8I | GTNNAC | 6 cut(s) | 551, 630, 651, 769, 863, 980 |
| HpyAV | CCTTC | 4 cut(s) | 434, 533, 572, 802 |
| HpyCH4III | ACNGT | 4 cut(s) | 599, 625, 802, 870 |
| HpyCH4IV | ACGT | 1 cut(s) | 1148 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 781, 884, 938, 1040, 1049 |
| HpyF3I | CTNAG | 5 cut(s) | 24, 218, 937, 1029, 1281 |
| HpySE526I | ACGT | 1 cut(s) | 1148 |
| Hsp92II | CATG | 7 cut(s) | 44, 145, 226, 306, 497, 700, 913 |
| Ksp22I | TGATCA | 2 cut(s) | 127, 693 |
| Kzo9I | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| LmnI | GCTCC | 3 cut(s) | 22, 324, 568 |
| Lsp1109I | GCAGC | 4 cut(s) | 328, 558, 762, 1003 |
| LweI | GCATC | 6 cut(s) | 193, 850, 874, 1003, 1169, 1207 |
| MaeI | CTAG | 2 cut(s) | 1152, 1205 |
| MaeII | ACGT | 1 cut(s) | 1148 |
| MaeIII | GTNAC | 5 cut(s) | 156, 236, 287, 309, 619 |
| MalI | GATC | 5 cut(s) | 118, 129, 395, 695, 925 |
| MboI | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| MboII | GAAGA | 7 cut(s) | 46, 77, 112, 344, 498, 822, 1211 |
| MfeI | CAATTG | 2 cut(s) | 60, 1080 |
| MhlI | GDGCHC | 4 cut(s) | 219, 493, 553, 730 |
| MluCI | AATT | 8 cut(s) | 60, 193, 278, 453, 826, 1035, 1080, 1093 |
| MlyI | GAGTC | 2 cut(s) | 497, 513 |
| MmeI | TCCRAC | 2 cut(s) | 44, 1114 |
| MnlI | CCTC | 8 cut(s) | 220, 223, 369, 469, 784, 947, 1024, 1130 |
| Mph1103I | ATGCAT | 1 cut(s) | 1162 |
| MseI | TTAA | 3 cut(s) | 183, 407, 1245 |
| MslI | CAYNNNNRTG | 2 cut(s) | 45, 165 |
| MspA1I | CMGCKG | 1 cut(s) | 133 |
| MunI | CAATTG | 2 cut(s) | 60, 1080 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 781, 884, 938, 1040, 1049 |
| NdeI | CATATG | 1 cut(s) | 273 |
| NdeII | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| NlaIII | CATG | 7 cut(s) | 44, 145, 226, 306, 497, 700, 913 |
| NlaIV | GGNNCC | 2 cut(s) | 245, 999 |
| NmuCI | GTSAC | 1 cut(s) | 619 |
| NsiI | ATGCAT | 1 cut(s) | 1162 |
| NspI | RCATGY | 1 cut(s) | 44 |
| PagI | TCATGA | 1 cut(s) | 141 |
| PfeI | GAWTC | 3 cut(s) | 674, 848, 1172 |
| PkrI | GCNGC | 6 cut(s) | 16, 318, 573, 589, 777, 1018 |
| PleI | GAGTC | 2 cut(s) | 497, 513 |
| PpsI | GAGTC | 2 cut(s) | 497, 513 |
| Psp124BI | GAGCTC | 1 cut(s) | 493 |
| PspFI | CCCAGC | 1 cut(s) | 654 |
| PspN4I | GGNNCC | 2 cut(s) | 245, 999 |
| PspPI | GGNCC | 1 cut(s) | 964 |
| PstI | CTGCAG | 1 cut(s) | 1045 |
| PstNI | CAGNNNCTG | 3 cut(s) | 125, 322, 1016 |
| RsaI | GTAC | 4 cut(s) | 81, 108, 906, 1274 |
| RsaNI | GTAC | 4 cut(s) | 80, 107, 905, 1273 |
| RseI | CAYNNNNRTG | 2 cut(s) | 45, 165 |
| SacI | GAGCTC | 1 cut(s) | 493 |
| SaqAI | TTAA | 3 cut(s) | 183, 407, 1245 |
| SatI | GCNGC | 6 cut(s) | 15, 317, 572, 588, 776, 1017 |
| Sau3AI | GATC | 5 cut(s) | 116, 127, 393, 693, 923 |
| Sau96I | GGNCC | 1 cut(s) | 964 |
| ScaI | AGTACT | 2 cut(s) | 81, 1274 |
| SchI | GAGTC | 2 cut(s) | 497, 513 |
| SduI | GDGCHC | 4 cut(s) | 219, 493, 553, 730 |
| SfaNI | GCATC | 6 cut(s) | 193, 850, 874, 1003, 1169, 1207 |
| SfcI | CTRYAG | 2 cut(s) | 866, 1041 |
| SinI | GGWCC | 1 cut(s) | 964 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 45, 165 |
| SmlI | CTYRAG | 1 cut(s) | 461 |
| SmoI | CTYRAG | 1 cut(s) | 461 |
| Sse9I | AATT | 8 cut(s) | 60, 193, 278, 453, 826, 1035, 1080, 1093 |
| SsiI | CCGC | 2 cut(s) | 133, 587 |
| SspMI | CTAG | 2 cut(s) | 1152, 1205 |
| SstI | GAGCTC | 1 cut(s) | 493 |
| TaaI | ACNGT | 4 cut(s) | 599, 625, 802, 870 |
| TaiI | ACGT | 1 cut(s) | 1151 |
| TaqI | TCGA | 3 cut(s) | 32, 416, 440 |
| TasI | AATT | 8 cut(s) | 60, 193, 278, 453, 826, 1035, 1080, 1093 |
| TatI | WGTACW | 3 cut(s) | 79, 106, 1272 |
| TauI | GCSGC | 1 cut(s) | 590 |
| TfiI | GAWTC | 3 cut(s) | 674, 848, 1172 |
| Tru1I | TTAA | 3 cut(s) | 183, 407, 1245 |
| Tru9I | TTAA | 3 cut(s) | 183, 407, 1245 |
| TscAI | CASTG | 7 cut(s) | 604, 624, 735, 807, 875, 1113, 1231 |
| TseFI | GTSAC | 1 cut(s) | 619 |
| TseI | GCWGC | 5 cut(s) | 14, 316, 571, 775, 1016 |
| Tsp45I | GTSAC | 1 cut(s) | 619 |
| TspDTI | ATGAA | 5 cut(s) | 42, 260, 290, 353, 687 |
| TspRI | CASTG | 7 cut(s) | 604, 624, 735, 807, 875, 1113, 1231 |
| VneI | GTGCAC | 1 cut(s) | 549 |
| VpaK11BI | GGWCC | 1 cut(s) | 964 |
| XapI | RAATTY | 3 cut(s) | 278, 453, 1093 |
| XceI | RCATGY | 1 cut(s) | 44 |
| XmiI | GTMKAC | 1 cut(s) | 768 |
| XspI | CTAG | 2 cut(s) | 1152, 1205 |
| ZrmI | AGTACT | 2 cut(s) | 81, 1274 |
| Zsp2I | ATGCAT | 1 cut(s) | 1162 |