NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 105 |
| AccB1I | GGYRCC | 1 cut(s) | 598 |
| AccI | GTMKAC | 2 cut(s) | 170, 580 |
| AciI | CCGC | 2 cut(s) | 533, 547 |
| AcoI | YGGCCR | 1 cut(s) | 241 |
| AcsI | RAATTY | 3 cut(s) | 36, 422, 694 |
| AcuI | CTGAAG | 5 cut(s) | 93, 284, 328, 474, 819 |
| AfaI | GTAC | 2 cut(s) | 134, 507 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 301, 631 |
| AgsI | TTSAA | 6 cut(s) | 68, 449, 497, 708, 747, 841 |
| AjnI | CCWGG | 1 cut(s) | 243 |
| AluBI | AGCT | 6 cut(s) | 74, 214, 298, 500, 542, 809 |
| AluI | AGCT | 6 cut(s) | 74, 214, 298, 500, 542, 809 |
| Alw21I | GWGCWC | 1 cut(s) | 76 |
| Alw26I | GTCTC | 2 cut(s) | 26, 199 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 307 |
| Ama87I | CYCGRG | 1 cut(s) | 589 |
| AoxI | GGCC | 1 cut(s) | 241 |
| ApeKI | GCWGC | 3 cut(s) | 214, 436, 617 |
| ApoI | RAATTY | 3 cut(s) | 36, 422, 694 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 562, 594 |
| AspS9I | GGNCC | 2 cut(s) | 565, 740 |
| AsuHPI | GGTGA | 1 cut(s) | 32 |
| AvaI | CYCGRG | 1 cut(s) | 589 |
| AvaII | GGWCC | 2 cut(s) | 565, 740 |
| BalI | TGGCCA | 1 cut(s) | 243 |
| BanI | GGYRCC | 1 cut(s) | 598 |
| BanII | GRGCYC | 1 cut(s) | 76 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 489, 521 |
| BauI | CACGAG | 1 cut(s) | 93 |
| Bbv12I | GWGCWC | 1 cut(s) | 76 |
| BbvI | GCAGC | 3 cut(s) | 201, 448, 604 |
| BciT130I | CCWGG | 1 cut(s) | 245 |
| BcoDI | GTCTC | 2 cut(s) | 26, 199 |
| BfaI | CTAG | 4 cut(s) | 405, 753, 806, 848 |
| BfmI | CTRYAG | 1 cut(s) | 642 |
| BfuAI | ACCTGC | 1 cut(s) | 105 |
| BisI | GCNGC | 3 cut(s) | 215, 437, 618 |
| BlsI | GCNGC | 3 cut(s) | 216, 438, 619 |
| Bme1390I | CCNGG | 1 cut(s) | 245 |
| Bme18I | GGWCC | 2 cut(s) | 565, 740 |
| BmeT110I | CYCGRG | 1 cut(s) | 589 |
| BmgT120I | GGNCC | 2 cut(s) | 565, 740 |
| BmiI | GGNNCC | 1 cut(s) | 600 |
| BmrFI | CCNGG | 1 cut(s) | 245 |
| BmsI | GCATC | 7 cut(s) | 21, 346, 405, 475, 640, 763, 770 |
| BpmI | CTGGAG | 1 cut(s) | 230 |
| BpuEI | CTTGAG | 2 cut(s) | 65, 795 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 301, 631 |
| Bse1I | ACTGG | 1 cut(s) | 213 |
| Bse3DI | GCAATG | 1 cut(s) | 687 |
| BseBI | CCWGG | 1 cut(s) | 245 |
| BseGI | GGATG | 2 cut(s) | 381, 823 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 301, 631 |
| BseMI | GCAATG | 1 cut(s) | 687 |
| BseMII | CTCAG | 2 cut(s) | 39, 621 |
| BseNI | ACTGG | 1 cut(s) | 213 |
| BseXI | GCAGC | 3 cut(s) | 201, 448, 604 |
| BsgI | GTGCAG | 2 cut(s) | 141, 261 |
| Bsh1285I | CGRYCG | 1 cut(s) | 537 |
| BshFI | GGCC | 1 cut(s) | 243 |
| BshNI | GGYRCC | 1 cut(s) | 598 |
| BsiEI | CGRYCG | 1 cut(s) | 537 |
| BsiHKAI | GWGCWC | 1 cut(s) | 76 |
| BsiHKCI | CYCGRG | 1 cut(s) | 589 |
| BslFI | GGGAC | 2 cut(s) | 571, 606 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 301, 631 |
| BsmAI | GTCTC | 2 cut(s) | 26, 199 |
| BsmFI | GGGAC | 2 cut(s) | 571, 606 |
| BsnI | GGCC | 1 cut(s) | 243 |
| BsoBI | CYCGRG | 1 cut(s) | 589 |
| Bsp1286I | GDGCHC | 1 cut(s) | 76 |
| Bsp143I | GATC | 1 cut(s) | 524 |
| BspACI | CCGC | 2 cut(s) | 533, 547 |
| BspANI | GGCC | 1 cut(s) | 243 |
| BspCNI | CTCAG | 2 cut(s) | 38, 622 |
| BspLI | GGNNCC | 1 cut(s) | 600 |
| BspMAI | CTGCAG | 1 cut(s) | 646 |
| BspMI | ACCTGC | 1 cut(s) | 105 |
| BspT107I | GGYRCC | 1 cut(s) | 598 |
| BsrDI | GCAATG | 1 cut(s) | 687 |
| BsrI | ACTGG | 1 cut(s) | 213 |
| BssMI | GATC | 1 cut(s) | 524 |
| BssSI | CACGAG | 1 cut(s) | 93 |
| Bst2BI | CACGAG | 1 cut(s) | 93 |
| Bst2UI | CCWGG | 1 cut(s) | 245 |
| Bst4CI | ACNGT | 2 cut(s) | 20, 471 |
| Bst6I | CTCTTC | 1 cut(s) | 94 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 485, 641, 650 |
| BstC8I | GCNNGC | 2 cut(s) | 296, 434 |
| BstDEI | CTNAG | 4 cut(s) | 5, 25, 161, 630 |
| BstF5I | GGATG | 2 cut(s) | 381, 823 |
| BstKTI | GATC | 1 cut(s) | 527 |
| BstMAI | GTCTC | 2 cut(s) | 26, 199 |
| BstMBI | GATC | 1 cut(s) | 524 |
| BstMCI | CGRYCG | 1 cut(s) | 537 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 304, 365, 485, 539, 641, 650, 718 |
| BstNI | CCWGG | 1 cut(s) | 245 |
| BstSCI | CCNGG | 1 cut(s) | 243 |
| BstSFI | CTRYAG | 1 cut(s) | 642 |
| BstV1I | GCAGC | 3 cut(s) | 201, 448, 604 |
| BsuRI | GGCC | 1 cut(s) | 243 |
| BtsCI | GGATG | 2 cut(s) | 381, 823 |
| BtsI | GCAGTG | 1 cut(s) | 375 |
| BtsIMutI | CAGTG | 4 cut(s) | 33, 244, 375, 476 |
| BveI | ACCTGC | 1 cut(s) | 105 |
| Cac8I | GCNNGC | 2 cut(s) | 296, 434 |
| CaiI | CAGNNNCTG | 1 cut(s) | 307 |
| Cfr13I | GGNCC | 2 cut(s) | 565, 740 |
| Csp6I | GTAC | 2 cut(s) | 133, 506 |
| CviAII | CATG | 3 cut(s) | 77, 337, 511 |
| CviQI | GTAC | 2 cut(s) | 133, 506 |
| DdeI | CTNAG | 4 cut(s) | 5, 25, 161, 630 |
| DpnI | GATC | 1 cut(s) | 526 |
| DpnII | GATC | 1 cut(s) | 524 |
| EaeI | YGGCCR | 1 cut(s) | 241 |
| Eam1104I | CTCTTC | 1 cut(s) | 94 |
| EarI | CTCTTC | 1 cut(s) | 94 |
| Ecl136II | GAGCTC | 1 cut(s) | 74 |
| Eco24I | GRGCYC | 1 cut(s) | 76 |
| Eco47I | GGWCC | 2 cut(s) | 565, 740 |
| Eco53kI | GAGCTC | 1 cut(s) | 74 |
| Eco57I | CTGAAG | 5 cut(s) | 93, 284, 328, 474, 819 |
| Eco88I | CYCGRG | 1 cut(s) | 589 |
| EcoICRI | GAGCTC | 1 cut(s) | 74 |
| EcoO109I | RGGNCCY | 1 cut(s) | 740 |
| EcoRI | GAATTC | 1 cut(s) | 36 |
| EcoRII | CCWGG | 1 cut(s) | 243 |
| EcoT22I | ATGCAT | 1 cut(s) | 763 |
| EcoT38I | GRGCYC | 1 cut(s) | 76 |
| FaeI | CATG | 3 cut(s) | 80, 340, 514 |
| FaiI | YATR | 8 cut(s) | 78, 195, 329, 338, 512, 604, 833, 865 |
| FaqI | GGGAC | 2 cut(s) | 571, 606 |
| FatI | CATG | 3 cut(s) | 76, 336, 510 |
| FauI | CCCGC | 1 cut(s) | 540 |
| FblI | GTMKAC | 2 cut(s) | 170, 580 |
| Fnu4HI | GCNGC | 3 cut(s) | 215, 437, 618 |
| FokI | GGATG | 2 cut(s) | 388, 830 |
| FriOI | GRGCYC | 1 cut(s) | 76 |
| Fsp4HI | GCNGC | 3 cut(s) | 215, 437, 618 |
| FspBI | CTAG | 4 cut(s) | 405, 753, 806, 848 |
| GluI | GCNGC | 3 cut(s) | 215, 437, 618 |
| GsuI | CTGGAG | 1 cut(s) | 230 |
| HaeIII | GGCC | 1 cut(s) | 243 |
| Hin1II | CATG | 3 cut(s) | 80, 340, 514 |
| HincII | GTYRAC | 2 cut(s) | 270, 581 |
| HindII | GTYRAC | 2 cut(s) | 270, 581 |
| HindIII | AAGCTT | 1 cut(s) | 540 |
| HinfI | GANTC | 5 cut(s) | 86, 103, 185, 314, 449 |
| HphI | GGTGA | 1 cut(s) | 32 |
| Hpy166II | GTNNAC | 5 cut(s) | 133, 171, 270, 464, 581 |
| Hpy188I | TCNGA | 5 cut(s) | 145, 190, 631, 673, 766 |
| Hpy188III | TCNNGA | 6 cut(s) | 65, 126, 386, 446, 591, 848 |
| Hpy8I | GTNNAC | 5 cut(s) | 133, 171, 270, 464, 581 |
| HpyAV | CCTTC | 7 cut(s) | 53, 117, 215, 256, 753, 780, 847 |
| HpyCH4III | ACNGT | 2 cut(s) | 20, 471 |
| HpyCH4IV | ACGT | 1 cut(s) | 749 |
| HpyCH4V | TGCA | 9 cut(s) | 158, 278, 359, 396, 436, 644, 692, 761, 776 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 304, 365, 485, 539, 641, 650, 718 |
| HpyF3I | CTNAG | 4 cut(s) | 5, 25, 161, 630 |
| HpySE526I | ACGT | 1 cut(s) | 749 |
| Hsp92II | CATG | 3 cut(s) | 80, 340, 514 |
| Kzo9I | GATC | 1 cut(s) | 524 |
| LmnI | GCTCC | 1 cut(s) | 211 |
| Lsp1109I | GCAGC | 3 cut(s) | 201, 448, 604 |
| LweI | GCATC | 7 cut(s) | 21, 346, 405, 475, 640, 763, 770 |
| MaeI | CTAG | 4 cut(s) | 405, 753, 806, 848 |
| MaeII | ACGT | 1 cut(s) | 749 |
| MalI | GATC | 1 cut(s) | 526 |
| MboI | GATC | 1 cut(s) | 524 |
| MboII | GAAGA | 2 cut(s) | 81, 812 |
| MhlI | GDGCHC | 1 cut(s) | 76 |
| MlsI | TGGCCA | 1 cut(s) | 243 |
| MluCI | AATT | 4 cut(s) | 36, 422, 636, 694 |
| MluNI | TGGCCA | 1 cut(s) | 243 |
| MlyI | GAGTC | 2 cut(s) | 80, 97 |
| MnlI | CCTC | 4 cut(s) | 366, 548, 625, 731 |
| Mox20I | TGGCCA | 1 cut(s) | 243 |
| Mph1103I | ATGCAT | 1 cut(s) | 763 |
| MscI | TGGCCA | 1 cut(s) | 243 |
| MseI | TTAA | 2 cut(s) | 99, 420 |
| Msp20I | TGGCCA | 1 cut(s) | 243 |
| MspR9I | CCNGG | 1 cut(s) | 245 |
| MvaI | CCWGG | 1 cut(s) | 245 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 304, 365, 485, 539, 641, 650, 718 |
| NdeII | GATC | 1 cut(s) | 524 |
| NlaIII | CATG | 3 cut(s) | 80, 340, 514 |
| NlaIV | GGNNCC | 1 cut(s) | 600 |
| NsiI | ATGCAT | 1 cut(s) | 763 |
| PfeI | GAWTC | 3 cut(s) | 185, 314, 449 |
| PkrI | GCNGC | 3 cut(s) | 216, 438, 619 |
| PleI | GAGTC | 2 cut(s) | 80, 97 |
| PpsI | GAGTC | 2 cut(s) | 80, 97 |
| PpuMI | RGGWCCY | 1 cut(s) | 740 |
| Psp124BI | GAGCTC | 1 cut(s) | 76 |
| Psp5II | RGGWCCY | 1 cut(s) | 740 |
| Psp6I | CCWGG | 1 cut(s) | 243 |
| PspGI | CCWGG | 1 cut(s) | 243 |
| PspN4I | GGNNCC | 1 cut(s) | 600 |
| PspPI | GGNCC | 2 cut(s) | 565, 740 |
| PspPPI | RGGWCCY | 1 cut(s) | 740 |
| PstI | CTGCAG | 1 cut(s) | 646 |
| PstNI | CAGNNNCTG | 1 cut(s) | 307 |
| RsaI | GTAC | 2 cut(s) | 134, 507 |
| RsaNI | GTAC | 2 cut(s) | 133, 506 |
| SacI | GAGCTC | 1 cut(s) | 76 |
| SalI | GTCGAC | 1 cut(s) | 579 |
| SaqAI | TTAA | 2 cut(s) | 99, 420 |
| SatI | GCNGC | 3 cut(s) | 215, 437, 618 |
| Sau3AI | GATC | 1 cut(s) | 524 |
| Sau96I | GGNCC | 2 cut(s) | 565, 740 |
| SchI | GAGTC | 2 cut(s) | 80, 97 |
| ScrFI | CCNGG | 1 cut(s) | 245 |
| SduI | GDGCHC | 1 cut(s) | 76 |
| SfaNI | GCATC | 7 cut(s) | 21, 346, 405, 475, 640, 763, 770 |
| SfcI | CTRYAG | 1 cut(s) | 642 |
| SinI | GGWCC | 2 cut(s) | 565, 740 |
| SmlI | CTYRAG | 2 cut(s) | 44, 810 |
| SmoI | CTYRAG | 2 cut(s) | 44, 810 |
| Sse9I | AATT | 4 cut(s) | 36, 422, 636, 694 |
| SsiI | CCGC | 2 cut(s) | 533, 547 |
| SspMI | CTAG | 4 cut(s) | 405, 753, 806, 848 |
| SstI | GAGCTC | 1 cut(s) | 76 |
| StyD4I | CCNGG | 1 cut(s) | 243 |
| TaaI | ACNGT | 2 cut(s) | 20, 471 |
| TaiI | ACGT | 1 cut(s) | 752 |
| TaqI | TCGA | 3 cut(s) | 537, 580, 648 |
| TasI | AATT | 4 cut(s) | 36, 422, 636, 694 |
| TatI | WGTACW | 1 cut(s) | 132 |
| TfiI | GAWTC | 3 cut(s) | 185, 314, 449 |
| Tru1I | TTAA | 2 cut(s) | 99, 420 |
| Tru9I | TTAA | 2 cut(s) | 99, 420 |
| TscAI | CASTG | 4 cut(s) | 33, 244, 375, 476 |
| TseI | GCWGC | 3 cut(s) | 214, 436, 617 |
| TspDTI | ATGAA | 4 cut(s) | 275, 327, 425, 813 |
| TspRI | CASTG | 4 cut(s) | 33, 244, 375, 476 |
| VpaK11BI | GGWCC | 2 cut(s) | 565, 740 |
| XapI | RAATTY | 3 cut(s) | 36, 422, 694 |
| XbaI | TCTAGA | 1 cut(s) | 847 |
| XmiI | GTMKAC | 2 cut(s) | 170, 580 |
| XspI | CTAG | 4 cut(s) | 405, 753, 806, 848 |
| Zsp2I | ATGCAT | 1 cut(s) | 763 |