NEDD8 ultimate buster
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 454 |
| AccI | GTMKAC | 1 cut(s) | 92 |
| AciI | CCGC | 2 cut(s) | 88, 152 |
| AclWI | GGATC | 1 cut(s) | 79 |
| AcsI | RAATTY | 1 cut(s) | 550 |
| AcuI | CTGAAG | 3 cut(s) | 254, 330, 675 |
| AfaI | GTAC | 4 cut(s) | 36, 63, 363, 731 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 487 |
| AgsI | TTSAA | 5 cut(s) | 25, 305, 353, 603, 698 |
| AluBI | AGCT | 4 cut(s) | 136, 225, 356, 398 |
| AluI | AGCT | 4 cut(s) | 136, 225, 356, 398 |
| Alw26I | GTCTC | 1 cut(s) | 71 |
| AlwI | GGATC | 1 cut(s) | 79 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 80, 473 |
| Ama87I | CYCGRG | 1 cut(s) | 445 |
| AoxI | GGCC | 1 cut(s) | 369 |
| ApeKI | GCWGC | 4 cut(s) | 136, 229, 295, 473 |
| ApoI | RAATTY | 1 cut(s) | 550 |
| AspS9I | GGNCC | 1 cut(s) | 421 |
| AsuC2I | CCSGG | 1 cut(s) | 220 |
| AsuHPI | GGTGA | 2 cut(s) | 208, 725 |
| AvaI | CYCGRG | 1 cut(s) | 445 |
| AvaII | GGWCC | 1 cut(s) | 421 |
| BanI | GGYRCC | 1 cut(s) | 454 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 345, 377 |
| BbvI | GCAGC | 4 cut(s) | 123, 216, 307, 460 |
| BclI | TGATCA | 1 cut(s) | 82 |
| BcnI | CCSGG | 1 cut(s) | 220 |
| BcoDI | GTCTC | 1 cut(s) | 71 |
| BfaI | CTAG | 2 cut(s) | 609, 662 |
| BfmI | CTRYAG | 2 cut(s) | 323, 498 |
| BisI | GCNGC | 5 cut(s) | 137, 153, 230, 296, 474 |
| BlsI | GCNGC | 5 cut(s) | 138, 154, 231, 297, 475 |
| BmcAI | AGTACT | 2 cut(s) | 36, 731 |
| Bme1390I | CCNGG | 1 cut(s) | 220 |
| Bme18I | GGWCC | 1 cut(s) | 421 |
| BmeT110I | CYCGRG | 1 cut(s) | 445 |
| BmgT120I | GGNCC | 1 cut(s) | 421 |
| BmiI | GGNNCC | 1 cut(s) | 456 |
| BmrFI | CCNGG | 1 cut(s) | 220 |
| BmsI | GCATC | 5 cut(s) | 307, 331, 460, 626, 664 |
| BpmI | CTGGAG | 1 cut(s) | 152 |
| BpuMI | CCSGG | 1 cut(s) | 220 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 487 |
| Bse1I | ACTGG | 1 cut(s) | 135 |
| Bse3DI | GCAATG | 2 cut(s) | 13, 543 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 487 |
| BseMI | GCAATG | 2 cut(s) | 13, 543 |
| BseMII | CTCAG | 1 cut(s) | 477 |
| BseNI | ACTGG | 1 cut(s) | 135 |
| BseXI | GCAGC | 4 cut(s) | 123, 216, 307, 460 |
| BsgI | GTGCAG | 1 cut(s) | 186 |
| BshFI | GGCC | 1 cut(s) | 371 |
| BshNI | GGYRCC | 1 cut(s) | 454 |
| BsiHKCI | CYCGRG | 1 cut(s) | 445 |
| BsiSI | CCGG | 1 cut(s) | 220 |
| BslFI | GGGAC | 2 cut(s) | 427, 462 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 487 |
| BsmAI | GTCTC | 1 cut(s) | 71 |
| BsmFI | GGGAC | 2 cut(s) | 427, 462 |
| BsnI | GGCC | 1 cut(s) | 371 |
| BsoBI | CYCGRG | 1 cut(s) | 445 |
| Bsp143I | GATC | 3 cut(s) | 71, 82, 380 |
| BspACI | CCGC | 2 cut(s) | 88, 152 |
| BspANI | GGCC | 1 cut(s) | 371 |
| BspCNI | CTCAG | 1 cut(s) | 478 |
| BspLI | GGNNCC | 1 cut(s) | 456 |
| BspMAI | CTGCAG | 1 cut(s) | 502 |
| BspPI | GGATC | 1 cut(s) | 79 |
| BspT107I | GGYRCC | 1 cut(s) | 454 |
| BsrDI | GCAATG | 2 cut(s) | 13, 543 |
| BsrI | ACTGG | 1 cut(s) | 135 |
| BssMI | GATC | 3 cut(s) | 71, 82, 380 |
| Bst4CI | ACNGT | 4 cut(s) | 164, 190, 256, 327 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 341, 497, 506 |
| BstC8I | GCNNGC | 1 cut(s) | 227 |
| BstDEI | CTNAG | 3 cut(s) | 394, 486, 738 |
| BstKTI | GATC | 3 cut(s) | 74, 85, 383 |
| BstMAI | GTCTC | 1 cut(s) | 71 |
| BstMBI | GATC | 3 cut(s) | 71, 82, 380 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 235, 341, 395, 497, 506 |
| BstSCI | CCNGG | 1 cut(s) | 218 |
| BstSFI | CTRYAG | 2 cut(s) | 323, 498 |
| BstV1I | GCAGC | 4 cut(s) | 123, 216, 307, 460 |
| BsuRI | GGCC | 1 cut(s) | 371 |
| BtsI | GCAGTG | 1 cut(s) | 437 |
| BtsIMutI | CAGTG | 6 cut(s) | 169, 261, 332, 437, 570, 681 |
| Cac8I | GCNNGC | 1 cut(s) | 227 |
| CaiI | CAGNNNCTG | 2 cut(s) | 80, 473 |
| Cfr13I | GGNCC | 1 cut(s) | 421 |
| CseI | GACGC | 1 cut(s) | 549 |
| Csp6I | GTAC | 4 cut(s) | 35, 62, 362, 730 |
| CviAII | CATG | 1 cut(s) | 367 |
| CviJI | RGCY | 7 cut(s) | 136, 225, 356, 371, 398, 645, 737 |
| CviKI_1 | RGCY | 7 cut(s) | 136, 225, 356, 371, 398, 645, 737 |
| CviQI | GTAC | 4 cut(s) | 35, 62, 362, 730 |
| DdeI | CTNAG | 3 cut(s) | 394, 486, 738 |
| DpnI | GATC | 3 cut(s) | 73, 84, 382 |
| DpnII | GATC | 3 cut(s) | 71, 82, 380 |
| Eco47I | GGWCC | 1 cut(s) | 421 |
| Eco57I | CTGAAG | 3 cut(s) | 254, 330, 675 |
| Eco88I | CYCGRG | 1 cut(s) | 445 |
| EcoT22I | ATGCAT | 1 cut(s) | 619 |
| FaeI | CATG | 1 cut(s) | 370 |
| FaiI | YATR | 6 cut(s) | 117, 243, 290, 368, 690, 722 |
| FaqI | GGGAC | 2 cut(s) | 427, 462 |
| FatI | CATG | 1 cut(s) | 366 |
| FbaI | TGATCA | 1 cut(s) | 82 |
| FblI | GTMKAC | 1 cut(s) | 92 |
| Fnu4HI | GCNGC | 5 cut(s) | 137, 153, 230, 296, 474 |
| Fsp4HI | GCNGC | 5 cut(s) | 137, 153, 230, 296, 474 |
| FspBI | CTAG | 2 cut(s) | 609, 662 |
| GluI | GCNGC | 5 cut(s) | 137, 153, 230, 296, 474 |
| GsuI | CTGGAG | 1 cut(s) | 152 |
| HaeIII | GGCC | 1 cut(s) | 371 |
| HapII | CCGG | 1 cut(s) | 220 |
| HgaI | GACGC | 1 cut(s) | 549 |
| Hin1II | CATG | 1 cut(s) | 370 |
| HincII | GTYRAC | 1 cut(s) | 195 |
| HindII | GTYRAC | 1 cut(s) | 195 |
| HindIII | AAGCTT | 1 cut(s) | 354 |
| HinfI | GANTC | 3 cut(s) | 107, 305, 629 |
| HpaII | CCGG | 1 cut(s) | 220 |
| HphI | GGTGA | 2 cut(s) | 208, 725 |
| Hpy166II | GTNNAC | 5 cut(s) | 93, 195, 216, 320, 437 |
| Hpy188I | TCNGA | 6 cut(s) | 48, 76, 487, 529, 622, 634 |
| Hpy188III | TCNNGA | 2 cut(s) | 302, 447 |
| Hpy8I | GTNNAC | 5 cut(s) | 93, 195, 216, 320, 437 |
| HpyAV | CCTTC | 2 cut(s) | 137, 256 |
| HpyCH4III | ACNGT | 4 cut(s) | 164, 190, 256, 327 |
| HpyCH4IV | ACGT | 1 cut(s) | 605 |
| HpyCH4V | TGCA | 6 cut(s) | 203, 403, 500, 536, 548, 617 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 235, 341, 395, 497, 506 |
| HpyF3I | CTNAG | 3 cut(s) | 394, 486, 738 |
| HpySE526I | ACGT | 1 cut(s) | 605 |
| Hsp92II | CATG | 1 cut(s) | 370 |
| Ksp22I | TGATCA | 1 cut(s) | 82 |
| Kzo9I | GATC | 3 cut(s) | 71, 82, 380 |
| LmnI | GCTCC | 2 cut(s) | 133, 222 |
| LpnPI | CCDG | 7 cut(s) | 116, 125, 155, 233, 389, 437, 510 |
| Lsp1109I | GCAGC | 4 cut(s) | 123, 216, 307, 460 |
| LweI | GCATC | 5 cut(s) | 307, 331, 460, 626, 664 |
| MaeI | CTAG | 2 cut(s) | 609, 662 |
| MaeII | ACGT | 1 cut(s) | 605 |
| MalI | GATC | 3 cut(s) | 73, 84, 382 |
| MboI | GATC | 3 cut(s) | 71, 82, 380 |
| MboII | GAAGA | 4 cut(s) | 37, 67, 276, 668 |
| MfeI | CAATTG | 2 cut(s) | 20, 537 |
| MluCI | AATT | 5 cut(s) | 20, 280, 492, 537, 550 |
| MmeI | TCCRAC | 1 cut(s) | 571 |
| MnlI | CCTC | 4 cut(s) | 238, 404, 481, 587 |
| Mph1103I | ATGCAT | 1 cut(s) | 619 |
| MseI | TTAA | 1 cut(s) | 702 |
| MspA1I | CMGCKG | 1 cut(s) | 88 |
| MspI | CCGG | 1 cut(s) | 220 |
| MspR9I | CCNGG | 1 cut(s) | 220 |
| MunI | CAATTG | 2 cut(s) | 20, 537 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 235, 341, 395, 497, 506 |
| NciI | CCSGG | 1 cut(s) | 220 |
| NdeII | GATC | 3 cut(s) | 71, 82, 380 |
| NlaIII | CATG | 1 cut(s) | 370 |
| NlaIV | GGNNCC | 1 cut(s) | 456 |
| NsiI | ATGCAT | 1 cut(s) | 619 |
| PfeI | GAWTC | 3 cut(s) | 107, 305, 629 |
| PkrI | GCNGC | 5 cut(s) | 138, 154, 231, 297, 475 |
| PspN4I | GGNNCC | 1 cut(s) | 456 |
| PspPI | GGNCC | 1 cut(s) | 421 |
| PstI | CTGCAG | 1 cut(s) | 502 |
| PstNI | CAGNNNCTG | 2 cut(s) | 80, 473 |
| RsaI | GTAC | 4 cut(s) | 36, 63, 363, 731 |
| RsaNI | GTAC | 4 cut(s) | 35, 62, 362, 730 |
| SaqAI | TTAA | 1 cut(s) | 702 |
| SatI | GCNGC | 5 cut(s) | 137, 153, 230, 296, 474 |
| Sau3AI | GATC | 3 cut(s) | 71, 82, 380 |
| Sau96I | GGNCC | 1 cut(s) | 421 |
| ScaI | AGTACT | 2 cut(s) | 36, 731 |
| ScrFI | CCNGG | 1 cut(s) | 220 |
| SetI | ASST | 8 cut(s) | 138, 174, 227, 358, 400, 598, 608, 715 |
| SfaNI | GCATC | 5 cut(s) | 307, 331, 460, 626, 664 |
| SfcI | CTRYAG | 2 cut(s) | 323, 498 |
| SinI | GGWCC | 1 cut(s) | 421 |
| Sse9I | AATT | 5 cut(s) | 20, 280, 492, 537, 550 |
| SsiI | CCGC | 2 cut(s) | 88, 152 |
| SspMI | CTAG | 2 cut(s) | 609, 662 |
| StyD4I | CCNGG | 1 cut(s) | 218 |
| TaaI | ACNGT | 4 cut(s) | 164, 190, 256, 327 |
| TaiI | ACGT | 1 cut(s) | 608 |
| TasI | AATT | 5 cut(s) | 20, 280, 492, 537, 550 |
| TatI | WGTACW | 3 cut(s) | 34, 61, 729 |
| TauI | GCSGC | 1 cut(s) | 155 |
| TfiI | GAWTC | 3 cut(s) | 107, 305, 629 |
| Tru1I | TTAA | 1 cut(s) | 702 |
| Tru9I | TTAA | 1 cut(s) | 702 |
| TscAI | CASTG | 6 cut(s) | 169, 261, 332, 444, 570, 688 |
| TseI | GCWGC | 4 cut(s) | 136, 229, 295, 473 |
| TspRI | CASTG | 6 cut(s) | 169, 261, 332, 444, 570, 688 |
| VpaK11BI | GGWCC | 1 cut(s) | 421 |
| XapI | RAATTY | 1 cut(s) | 550 |
| XmiI | GTMKAC | 1 cut(s) | 92 |
| XspI | CTAG | 2 cut(s) | 609, 662 |
| ZrmI | AGTACT | 2 cut(s) | 36, 731 |
| Zsp2I | ATGCAT | 1 cut(s) | 619 |