NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 814 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 547 |
| AccI | GTMKAC | 2 cut(s) | 73, 108 |
| AciI | CCGC | 2 cut(s) | 256, 473 |
| AclWI | GGATC | 3 cut(s) | 26, 247, 368 |
| AcsI | RAATTY | 3 cut(s) | 7, 554, 910 |
| AcuI | CTGAAG | 5 cut(s) | 417, 562, 614, 690, 1035 |
| AfaI | GTAC | 3 cut(s) | 231, 723, 1091 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 132, 547, 579, 847 |
| AgsI | TTSAA | 7 cut(s) | 26, 188, 196, 665, 713, 963, 1058 |
| AjnI | CCWGG | 1 cut(s) | 540 |
| AleI | CACNNNNGTG | 1 cut(s) | 542 |
| AluBI | AGCT | 8 cut(s) | 22, 391, 457, 576, 585, 716, 758, 1037 |
| AluI | AGCT | 8 cut(s) | 22, 391, 457, 576, 585, 716, 758, 1037 |
| Alw21I | GWGCWC | 1 cut(s) | 342 |
| Alw26I | GTCTC | 1 cut(s) | 154 |
| AlwI | GGATC | 3 cut(s) | 26, 247, 368 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 547 |
| Ama87I | CYCGRG | 1 cut(s) | 805 |
| AoxI | GGCC | 2 cut(s) | 42, 729 |
| ApeKI | GCWGC | 6 cut(s) | 19, 137, 457, 589, 655, 833 |
| ApoI | RAATTY | 3 cut(s) | 7, 554, 910 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 101, 133 |
| AspLEI | GCGC | 1 cut(s) | 151 |
| AspS9I | GGNCC | 1 cut(s) | 781 |
| AsuHPI | GGTGA | 2 cut(s) | 529, 1085 |
| AvaI | CYCGRG | 1 cut(s) | 805 |
| AvaII | GGWCC | 1 cut(s) | 781 |
| BanI | GGYRCC | 1 cut(s) | 814 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 705, 737 |
| BbsI | GAAGAC | 1 cut(s) | 98 |
| Bbv12I | GWGCWC | 1 cut(s) | 342 |
| BbvI | GCAGC | 6 cut(s) | 31, 124, 444, 576, 667, 820 |
| BccI | CCATC | 2 cut(s) | 164, 385 |
| BceAI | ACGGC | 1 cut(s) | 71 |
| BciT130I | CCWGG | 1 cut(s) | 542 |
| BclI | TGATCA | 1 cut(s) | 250 |
| BcoDI | GTCTC | 1 cut(s) | 154 |
| BfaI | CTAG | 2 cut(s) | 969, 1022 |
| BfmI | CTRYAG | 2 cut(s) | 683, 858 |
| BisI | GCNGC | 7 cut(s) | 20, 138, 458, 474, 590, 656, 834 |
| BlpI | GCTNAGC | 1 cut(s) | 341 |
| BlsI | GCNGC | 7 cut(s) | 21, 139, 459, 475, 591, 657, 835 |
| BmcAI | AGTACT | 1 cut(s) | 1091 |
| Bme1390I | CCNGG | 1 cut(s) | 542 |
| Bme18I | GGWCC | 1 cut(s) | 781 |
| BmeT110I | CYCGRG | 1 cut(s) | 805 |
| BmgT120I | GGNCC | 1 cut(s) | 781 |
| BmiI | GGNNCC | 1 cut(s) | 816 |
| BmrFI | CCNGG | 1 cut(s) | 542 |
| BmsI | GCATC | 6 cut(s) | 73, 316, 667, 691, 820, 986 |
| BpiI | GAAGAC | 1 cut(s) | 98 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 347, 379 |
| BpmI | CTGGAG | 1 cut(s) | 473 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 341 |
| Bsa29I | ATCGAT | 1 cut(s) | 420 |
| BsaJI | CCNNGG | 1 cut(s) | 540 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 132, 547, 579, 847 |
| Bse1I | ACTGG | 2 cut(s) | 126, 456 |
| Bse3DI | GCAATG | 4 cut(s) | 60, 176, 336, 903 |
| BseBI | CCWGG | 1 cut(s) | 542 |
| BseCI | ATCGAT | 1 cut(s) | 420 |
| BseDI | CCNNGG | 1 cut(s) | 540 |
| BseGI | GGATG | 1 cut(s) | 556 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 132, 547, 579, 847 |
| BseMI | GCAATG | 4 cut(s) | 60, 176, 336, 903 |
| BseMII | CTCAG | 3 cut(s) | 126, 355, 837 |
| BseNI | ACTGG | 2 cut(s) | 126, 456 |
| BseRI | GAGGAG | 2 cut(s) | 365, 368 |
| BseXI | GCAGC | 6 cut(s) | 31, 124, 444, 576, 667, 820 |
| BsgI | GTGCAG | 1 cut(s) | 507 |
| BshFI | GGCC | 2 cut(s) | 44, 731 |
| BshNI | GGYRCC | 1 cut(s) | 814 |
| BshVI | ATCGAT | 1 cut(s) | 420 |
| BsiHKAI | GWGCWC | 1 cut(s) | 342 |
| BsiHKCI | CYCGRG | 1 cut(s) | 805 |
| BslFI | GGGAC | 2 cut(s) | 787, 822 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 132, 547, 579, 847 |
| BsmAI | GTCTC | 1 cut(s) | 154 |
| BsmFI | GGGAC | 2 cut(s) | 787, 822 |
| BsnI | GGCC | 2 cut(s) | 44, 731 |
| BsoBI | CYCGRG | 1 cut(s) | 805 |
| Bsp1286I | GDGCHC | 1 cut(s) | 342 |
| Bsp143I | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 341 |
| BspACI | CCGC | 2 cut(s) | 256, 473 |
| BspANI | GGCC | 2 cut(s) | 44, 731 |
| BspCNI | CTCAG | 3 cut(s) | 125, 354, 838 |
| BspDI | ATCGAT | 1 cut(s) | 420 |
| BspHI | TCATGA | 1 cut(s) | 264 |
| BspLI | GGNNCC | 1 cut(s) | 816 |
| BspMAI | CTGCAG | 1 cut(s) | 862 |
| BspPI | GGATC | 3 cut(s) | 26, 247, 368 |
| BspT107I | GGYRCC | 1 cut(s) | 814 |
| BsrDI | GCAATG | 4 cut(s) | 60, 176, 336, 903 |
| BsrI | ACTGG | 2 cut(s) | 126, 456 |
| BssECI | CCNNGG | 1 cut(s) | 540 |
| BssMI | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| Bst2UI | CCWGG | 1 cut(s) | 542 |
| Bst4CI | ACNGT | 4 cut(s) | 485, 511, 616, 687 |
| Bst6I | CTCTTC | 1 cut(s) | 152 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 701, 857, 866 |
| BstC8I | GCNNGC | 2 cut(s) | 258, 587 |
| BstDEI | CTNAG | 5 cut(s) | 112, 341, 754, 846, 1098 |
| BstF5I | GGATG | 1 cut(s) | 556 |
| BstHHI | GCGC | 1 cut(s) | 151 |
| BstKTI | GATC | 5 cut(s) | 34, 242, 253, 376, 743 |
| BstMAI | GTCTC | 1 cut(s) | 154 |
| BstMBI | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 50, 146, 582, 595, 701, 755, 857, 866 |
| BstNI | CCWGG | 1 cut(s) | 542 |
| BstNSI | RCATGY | 1 cut(s) | 167 |
| BstSCI | CCNGG | 1 cut(s) | 540 |
| BstSFI | CTRYAG | 2 cut(s) | 683, 858 |
| BstV1I | GCAGC | 6 cut(s) | 31, 124, 444, 576, 667, 820 |
| BstV2I | GAAGAC | 1 cut(s) | 98 |
| Bsu15I | ATCGAT | 1 cut(s) | 420 |
| BsuRI | GGCC | 2 cut(s) | 44, 731 |
| BsuTUI | ATCGAT | 1 cut(s) | 420 |
| BtsCI | GGATG | 1 cut(s) | 556 |
| BtsIMutI | CAGTG | 4 cut(s) | 490, 621, 692, 1041 |
| Cac8I | GCNNGC | 2 cut(s) | 258, 587 |
| CaiI | CAGNNNCTG | 1 cut(s) | 547 |
| CciI | TCATGA | 1 cut(s) | 264 |
| CfoI | GCGC | 1 cut(s) | 151 |
| Cfr13I | GGNCC | 1 cut(s) | 781 |
| ClaI | ATCGAT | 1 cut(s) | 420 |
| Csp6I | GTAC | 3 cut(s) | 230, 722, 1090 |
| CviAII | CATG | 4 cut(s) | 164, 265, 346, 727 |
| CviQI | GTAC | 3 cut(s) | 230, 722, 1090 |
| DdeI | CTNAG | 5 cut(s) | 112, 341, 754, 846, 1098 |
| DpnI | GATC | 5 cut(s) | 33, 241, 252, 375, 742 |
| DpnII | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| Eam1104I | CTCTTC | 1 cut(s) | 152 |
| EarI | CTCTTC | 1 cut(s) | 152 |
| Eco147I | AGGCCT | 1 cut(s) | 44 |
| Eco47I | GGWCC | 1 cut(s) | 781 |
| Eco57I | CTGAAG | 5 cut(s) | 417, 562, 614, 690, 1035 |
| Eco88I | CYCGRG | 1 cut(s) | 805 |
| EcoRII | CCWGG | 1 cut(s) | 540 |
| EcoT22I | ATGCAT | 1 cut(s) | 979 |
| FaeI | CATG | 4 cut(s) | 167, 268, 349, 730 |
| FaqI | GGGAC | 2 cut(s) | 787, 822 |
| FatI | CATG | 4 cut(s) | 163, 264, 345, 726 |
| FauI | CCCGC | 1 cut(s) | 249 |
| FbaI | TGATCA | 1 cut(s) | 250 |
| FblI | GTMKAC | 2 cut(s) | 73, 108 |
| Fnu4HI | GCNGC | 7 cut(s) | 20, 138, 458, 474, 590, 656, 834 |
| FokI | GGATG | 1 cut(s) | 563 |
| Fsp4HI | GCNGC | 7 cut(s) | 20, 138, 458, 474, 590, 656, 834 |
| FspBI | CTAG | 2 cut(s) | 969, 1022 |
| GlaI | GCGC | 1 cut(s) | 150 |
| GluI | GCNGC | 7 cut(s) | 20, 138, 458, 474, 590, 656, 834 |
| GsuI | CTGGAG | 1 cut(s) | 473 |
| HaeIII | GGCC | 2 cut(s) | 44, 731 |
| HhaI | GCGC | 1 cut(s) | 151 |
| Hin1II | CATG | 4 cut(s) | 167, 268, 349, 730 |
| Hin6I | GCGC | 1 cut(s) | 149 |
| HinP1I | GCGC | 1 cut(s) | 149 |
| HincII | GTYRAC | 2 cut(s) | 516, 797 |
| HindII | GTYRAC | 2 cut(s) | 516, 797 |
| HindIII | AAGCTT | 2 cut(s) | 714, 1035 |
| HinfI | GANTC | 5 cut(s) | 110, 427, 569, 665, 989 |
| HphI | GGTGA | 2 cut(s) | 529, 1085 |
| Hpy166II | GTNNAC | 6 cut(s) | 74, 109, 516, 537, 680, 797 |
| Hpy188I | TCNGA | 5 cut(s) | 216, 847, 889, 982, 994 |
| Hpy188III | TCNNGA | 5 cut(s) | 265, 580, 662, 807, 1013 |
| Hpy8I | GTNNAC | 6 cut(s) | 74, 109, 516, 537, 680, 797 |
| HpyAV | CCTTC | 5 cut(s) | 95, 414, 441, 458, 616 |
| HpyCH4III | ACNGT | 4 cut(s) | 485, 511, 616, 687 |
| HpyCH4IV | ACGT | 1 cut(s) | 965 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 50, 146, 582, 595, 701, 755, 857, 866 |
| HpyF3I | CTNAG | 5 cut(s) | 112, 341, 754, 846, 1098 |
| HpySE526I | ACGT | 1 cut(s) | 965 |
| Hsp92II | CATG | 4 cut(s) | 167, 268, 349, 730 |
| HspAI | GCGC | 1 cut(s) | 149 |
| Ksp22I | TGATCA | 1 cut(s) | 250 |
| Kzo9I | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| LmnI | GCTCC | 3 cut(s) | 145, 454, 582 |
| Lsp1109I | GCAGC | 6 cut(s) | 31, 124, 444, 576, 667, 820 |
| LweI | GCATC | 6 cut(s) | 73, 316, 667, 691, 820, 986 |
| MaeI | CTAG | 2 cut(s) | 969, 1022 |
| MaeII | ACGT | 1 cut(s) | 965 |
| MaeIII | GTNAC | 2 cut(s) | 121, 279 |
| MalI | GATC | 5 cut(s) | 33, 241, 252, 375, 742 |
| MboI | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| MboII | GAAGA | 6 cut(s) | 103, 169, 200, 235, 636, 1028 |
| MfeI | CAATTG | 2 cut(s) | 183, 897 |
| MhlI | GDGCHC | 1 cut(s) | 342 |
| MluCI | AATT | 8 cut(s) | 7, 183, 316, 554, 640, 852, 897, 910 |
| MlyI | GAGTC | 2 cut(s) | 104, 421 |
| MmeI | TCCRAC | 2 cut(s) | 167, 931 |
| MnlI | CCTC | 7 cut(s) | 343, 346, 349, 598, 764, 841, 947 |
| Mph1103I | ATGCAT | 1 cut(s) | 979 |
| MseI | TTAA | 3 cut(s) | 306, 387, 1062 |
| MslI | CAYNNNNRTG | 3 cut(s) | 168, 288, 542 |
| MspA1I | CMGCKG | 1 cut(s) | 256 |
| MspR9I | CCNGG | 1 cut(s) | 542 |
| MunI | CAATTG | 2 cut(s) | 183, 897 |
| MvaI | CCWGG | 1 cut(s) | 542 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 50, 146, 582, 595, 701, 755, 857, 866 |
| NdeII | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| NlaIII | CATG | 4 cut(s) | 167, 268, 349, 730 |
| NlaIV | GGNNCC | 1 cut(s) | 816 |
| NmuCI | GTSAC | 1 cut(s) | 121 |
| NsiI | ATGCAT | 1 cut(s) | 979 |
| NspI | RCATGY | 1 cut(s) | 167 |
| OliI | CACNNNNGTG | 1 cut(s) | 542 |
| PagI | TCATGA | 1 cut(s) | 264 |
| PceI | AGGCCT | 1 cut(s) | 44 |
| PfeI | GAWTC | 3 cut(s) | 569, 665, 989 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 547 |
| PkrI | GCNGC | 7 cut(s) | 21, 139, 459, 475, 591, 657, 835 |
| PleI | GAGTC | 2 cut(s) | 104, 421 |
| PpsI | GAGTC | 2 cut(s) | 104, 421 |
| Psp6I | CCWGG | 1 cut(s) | 540 |
| PspGI | CCWGG | 1 cut(s) | 540 |
| PspN4I | GGNNCC | 1 cut(s) | 816 |
| PspPI | GGNCC | 1 cut(s) | 781 |
| PstI | CTGCAG | 1 cut(s) | 862 |
| PstNI | CAGNNNCTG | 1 cut(s) | 547 |
| RsaI | GTAC | 3 cut(s) | 231, 723, 1091 |
| RsaNI | GTAC | 3 cut(s) | 230, 722, 1090 |
| RseI | CAYNNNNRTG | 3 cut(s) | 168, 288, 542 |
| SaqAI | TTAA | 3 cut(s) | 306, 387, 1062 |
| SatI | GCNGC | 7 cut(s) | 20, 138, 458, 474, 590, 656, 834 |
| Sau3AI | GATC | 5 cut(s) | 31, 239, 250, 373, 740 |
| Sau96I | GGNCC | 1 cut(s) | 781 |
| ScaI | AGTACT | 1 cut(s) | 1091 |
| SchI | GAGTC | 2 cut(s) | 104, 421 |
| ScrFI | CCNGG | 1 cut(s) | 542 |
| SduI | GDGCHC | 1 cut(s) | 342 |
| SfaNI | GCATC | 6 cut(s) | 73, 316, 667, 691, 820, 986 |
| SfcI | CTRYAG | 2 cut(s) | 683, 858 |
| SinI | GGWCC | 1 cut(s) | 781 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 168, 288, 542 |
| Sse9I | AATT | 8 cut(s) | 7, 183, 316, 554, 640, 852, 897, 910 |
| SseBI | AGGCCT | 1 cut(s) | 44 |
| SsiI | CCGC | 2 cut(s) | 256, 473 |
| SspMI | CTAG | 2 cut(s) | 969, 1022 |
| StuI | AGGCCT | 1 cut(s) | 44 |
| StyD4I | CCNGG | 1 cut(s) | 540 |
| TaaI | ACNGT | 4 cut(s) | 485, 511, 616, 687 |
| TaiI | ACGT | 1 cut(s) | 968 |
| TaqI | TCGA | 3 cut(s) | 155, 396, 420 |
| TasI | AATT | 8 cut(s) | 7, 183, 316, 554, 640, 852, 897, 910 |
| TatI | WGTACW | 2 cut(s) | 229, 1089 |
| TauI | GCSGC | 1 cut(s) | 476 |
| TfiI | GAWTC | 3 cut(s) | 569, 665, 989 |
| Tru1I | TTAA | 3 cut(s) | 306, 387, 1062 |
| Tru9I | TTAA | 3 cut(s) | 306, 387, 1062 |
| TscAI | CASTG | 4 cut(s) | 490, 621, 692, 1048 |
| TseFI | GTSAC | 1 cut(s) | 121 |
| TseI | GCWGC | 6 cut(s) | 19, 137, 457, 589, 655, 833 |
| Tsp45I | GTSAC | 1 cut(s) | 121 |
| TspDTI | ATGAA | 3 cut(s) | 71, 165, 567 |
| TspRI | CASTG | 4 cut(s) | 490, 621, 692, 1048 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 547 |
| VpaK11BI | GGWCC | 1 cut(s) | 781 |
| XapI | RAATTY | 3 cut(s) | 7, 554, 910 |
| XceI | RCATGY | 1 cut(s) | 167 |
| XmiI | GTMKAC | 2 cut(s) | 73, 108 |
| XspI | CTAG | 2 cut(s) | 969, 1022 |
| ZrmI | AGTACT | 1 cut(s) | 1091 |
| Zsp2I | ATGCAT | 1 cut(s) | 979 |