NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 560, 641 |
| AccB1I | GGYRCC | 1 cut(s) | 1126 |
| AccI | GTMKAC | 1 cut(s) | 882 |
| AciI | CCGC | 3 cut(s) | 262, 707, 1373 |
| AclWI | GGATC | 2 cut(s) | 253, 383 |
| AcsI | RAATTY | 4 cut(s) | 8, 492, 1222, 1366 |
| AcuI | CTGAAG | 7 cut(s) | 548, 629, 764, 805, 914, 1002, 1296 |
| AfaI | GTAC | 5 cut(s) | 210, 237, 630, 759, 1035 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 724, 781, 1159 |
| AflIII | ACRYGT | 1 cut(s) | 631 |
| AgsI | TTSAA | 9 cut(s) | 194, 202, 215, 524, 601, 977, 1025, 1242, 1319 |
| AluBI | AGCT | 8 cut(s) | 15, 406, 530, 607, 691, 778, 1028, 1070 |
| AluI | AGCT | 8 cut(s) | 15, 406, 530, 607, 691, 778, 1028, 1070 |
| Alw21I | GWGCWC | 4 cut(s) | 348, 532, 609, 673 |
| Alw26I | GTCTC | 4 cut(s) | 160, 245, 459, 941 |
| Alw44I | GTGCAC | 1 cut(s) | 669 |
| AlwI | GGATC | 2 cut(s) | 253, 383 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 787, 1145 |
| Ama87I | CYCGRG | 1 cut(s) | 1117 |
| AoxI | GGCC | 1 cut(s) | 1041 |
| ApaLI | GTGCAC | 1 cut(s) | 669 |
| ApeKI | GCWGC | 3 cut(s) | 143, 691, 1145 |
| ApoI | RAATTY | 4 cut(s) | 8, 492, 1222, 1366 |
| AspS9I | GGNCC | 2 cut(s) | 1093, 1235 |
| AsuHPI | GGTGA | 3 cut(s) | 707, 763, 1346 |
| AvaI | CYCGRG | 1 cut(s) | 1117 |
| AvaII | GGWCC | 2 cut(s) | 1093, 1235 |
| BaeGI | GKGCMC | 2 cut(s) | 673, 847 |
| BanI | GGYRCC | 1 cut(s) | 1126 |
| BanII | GRGCYC | 2 cut(s) | 532, 609 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1017, 1049 |
| Bbv12I | GWGCWC | 4 cut(s) | 348, 532, 609, 673 |
| BbvI | GCAGC | 3 cut(s) | 130, 678, 1132 |
| BccI | CCATC | 3 cut(s) | 170, 400, 878 |
| BclI | TGATCA | 1 cut(s) | 256 |
| BcoDI | GTCTC | 4 cut(s) | 160, 245, 459, 941 |
| BfaI | CTAG | 3 cut(s) | 1248, 1283, 1326 |
| BfmI | CTRYAG | 2 cut(s) | 995, 1170 |
| BfuAI | ACCTGC | 2 cut(s) | 560, 641 |
| BisI | GCNGC | 4 cut(s) | 144, 692, 708, 1146 |
| BlpI | GCTNAGC | 1 cut(s) | 347 |
| BlsI | GCNGC | 4 cut(s) | 145, 693, 709, 1147 |
| BmcAI | AGTACT | 1 cut(s) | 210 |
| Bme18I | GGWCC | 2 cut(s) | 1093, 1235 |
| BmeT110I | CYCGRG | 1 cut(s) | 1117 |
| BmgT120I | GGNCC | 2 cut(s) | 1093, 1235 |
| BmiI | GGNNCC | 1 cut(s) | 1128 |
| BmsI | GCATC | 7 cut(s) | 322, 574, 879, 1003, 1132, 1265, 1285 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 364, 396 |
| BpmI | CTGGAG | 2 cut(s) | 391, 707 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 347 |
| BpuEI | CTTGAG | 1 cut(s) | 521 |
| Bsa29I | ATCGAT | 1 cut(s) | 435 |
| BsaAI | YACGTR | 1 cut(s) | 632 |
| BsaI | GGTCTC | 1 cut(s) | 941 |
| BsaJI | CCNNGG | 1 cut(s) | 718 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 724, 781, 1159 |
| Bse1I | ACTGG | 3 cut(s) | 374, 479, 690 |
| Bse3DI | GCAATG | 4 cut(s) | 65, 182, 342, 1215 |
| BseCI | ATCGAT | 1 cut(s) | 435 |
| BseDI | CCNNGG | 1 cut(s) | 718 |
| BseGI | GGATG | 2 cut(s) | 796, 858 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 724, 781, 1159 |
| BseMI | GCAATG | 4 cut(s) | 65, 182, 342, 1215 |
| BseMII | CTCAG | 4 cut(s) | 361, 495, 839, 1149 |
| BseNI | ACTGG | 3 cut(s) | 374, 479, 690 |
| BseRI | GAGGAG | 1 cut(s) | 371 |
| BseSI | GKGCMC | 2 cut(s) | 673, 847 |
| BseXI | GCAGC | 3 cut(s) | 130, 678, 1132 |
| BseYI | CCCAGC | 1 cut(s) | 774 |
| BshFI | GGCC | 1 cut(s) | 1043 |
| BshNI | GGYRCC | 1 cut(s) | 1126 |
| BshVI | ATCGAT | 1 cut(s) | 435 |
| BsiHKAI | GWGCWC | 4 cut(s) | 348, 532, 609, 673 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1117 |
| BslFI | GGGAC | 2 cut(s) | 1099, 1134 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 724, 781, 1159 |
| BsmAI | GTCTC | 4 cut(s) | 160, 245, 459, 941 |
| BsmFI | GGGAC | 2 cut(s) | 1099, 1134 |
| BsnI | GGCC | 1 cut(s) | 1043 |
| Bso31I | GGTCTC | 1 cut(s) | 941 |
| BsoBI | CYCGRG | 1 cut(s) | 1117 |
| Bsp1286I | GDGCHC | 5 cut(s) | 348, 532, 609, 673, 847 |
| Bsp1407I | TGTACA | 1 cut(s) | 757 |
| Bsp143I | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 347 |
| BspACI | CCGC | 3 cut(s) | 262, 707, 1373 |
| BspANI | GGCC | 1 cut(s) | 1043 |
| BspCNI | CTCAG | 4 cut(s) | 360, 494, 840, 1150 |
| BspDI | ATCGAT | 1 cut(s) | 435 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 1 cut(s) | 1128 |
| BspMAI | CTGCAG | 1 cut(s) | 1174 |
| BspMI | ACCTGC | 2 cut(s) | 560, 641 |
| BspPI | GGATC | 2 cut(s) | 253, 383 |
| BspT107I | GGYRCC | 1 cut(s) | 1126 |
| BspTNI | GGTCTC | 1 cut(s) | 941 |
| BsrDI | GCAATG | 4 cut(s) | 65, 182, 342, 1215 |
| BsrGI | TGTACA | 1 cut(s) | 757 |
| BsrI | ACTGG | 3 cut(s) | 374, 479, 690 |
| BssECI | CCNNGG | 1 cut(s) | 718 |
| BssMI | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| BssNAI | GTATAC | 1 cut(s) | 883 |
| Bst1107I | GTATAC | 1 cut(s) | 883 |
| Bst4CI | ACNGT | 4 cut(s) | 154, 719, 916, 999 |
| Bst6I | CTCTTC | 2 cut(s) | 158, 627 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1013, 1169, 1178 |
| BstAUI | TGTACA | 1 cut(s) | 757 |
| BstBAI | YACGTR | 1 cut(s) | 632 |
| BstC8I | GCNNGC | 2 cut(s) | 264, 669 |
| BstDEI | CTNAG | 6 cut(s) | 347, 461, 481, 848, 1066, 1158 |
| BstDSI | CCRYGG | 1 cut(s) | 718 |
| BstF5I | GGATG | 2 cut(s) | 796, 858 |
| BstKTI | GATC | 4 cut(s) | 248, 259, 391, 1055 |
| BstMAI | GTCTC | 4 cut(s) | 160, 245, 459, 941 |
| BstMBI | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 784, 1013, 1067, 1169, 1178 |
| BstNSI | RCATGY | 1 cut(s) | 173 |
| BstSFI | CTRYAG | 2 cut(s) | 995, 1170 |
| BstSLI | GKGCMC | 2 cut(s) | 673, 847 |
| BstV1I | GCAGC | 3 cut(s) | 130, 678, 1132 |
| BstZ17I | GTATAC | 1 cut(s) | 883 |
| Bsu15I | ATCGAT | 1 cut(s) | 435 |
| BsuRI | GGCC | 1 cut(s) | 1043 |
| BsuTUI | ATCGAT | 1 cut(s) | 435 |
| BtgI | CCRYGG | 1 cut(s) | 718 |
| BtsCI | GGATG | 2 cut(s) | 796, 858 |
| BtsI | GCAGTG | 1 cut(s) | 1109 |
| BtsIMutI | CAGTG | 6 cut(s) | 489, 845, 921, 1004, 1109, 1302 |
| BveI | ACCTGC | 2 cut(s) | 560, 641 |
| Cac8I | GCNNGC | 2 cut(s) | 264, 669 |
| CaiI | CAGNNNCTG | 2 cut(s) | 787, 1145 |
| CciI | TCATGA | 1 cut(s) | 270 |
| Cfr13I | GGNCC | 2 cut(s) | 1093, 1235 |
| ClaI | ATCGAT | 1 cut(s) | 435 |
| Csp6I | GTAC | 5 cut(s) | 209, 236, 629, 758, 1034 |
| CviAII | CATG | 7 cut(s) | 170, 271, 352, 457, 533, 610, 1039 |
| CviQI | GTAC | 5 cut(s) | 209, 236, 629, 758, 1034 |
| DdeI | CTNAG | 6 cut(s) | 347, 461, 481, 848, 1066, 1158 |
| DpnI | GATC | 4 cut(s) | 247, 258, 390, 1054 |
| DpnII | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| Eam1104I | CTCTTC | 2 cut(s) | 158, 627 |
| EarI | CTCTTC | 2 cut(s) | 158, 627 |
| Ecl136II | GAGCTC | 2 cut(s) | 530, 607 |
| Eco24I | GRGCYC | 2 cut(s) | 532, 609 |
| Eco31I | GGTCTC | 1 cut(s) | 941 |
| Eco47I | GGWCC | 2 cut(s) | 1093, 1235 |
| Eco53kI | GAGCTC | 2 cut(s) | 530, 607 |
| Eco57I | CTGAAG | 7 cut(s) | 548, 629, 764, 805, 914, 1002, 1296 |
| Eco88I | CYCGRG | 1 cut(s) | 1117 |
| EcoICRI | GAGCTC | 2 cut(s) | 530, 607 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1235 |
| EcoRI | GAATTC | 2 cut(s) | 492, 1366 |
| EcoT38I | GRGCYC | 2 cut(s) | 532, 609 |
| FaeI | CATG | 7 cut(s) | 173, 274, 355, 460, 536, 613, 1042 |
| FaqI | GGGAC | 2 cut(s) | 1099, 1134 |
| FatI | CATG | 7 cut(s) | 169, 270, 351, 456, 532, 609, 1038 |
| FauI | CCCGC | 2 cut(s) | 255, 1366 |
| FbaI | TGATCA | 1 cut(s) | 256 |
| FblI | GTMKAC | 1 cut(s) | 882 |
| Fnu4HI | GCNGC | 4 cut(s) | 144, 692, 708, 1146 |
| FokI | GGATG | 2 cut(s) | 803, 865 |
| FriOI | GRGCYC | 2 cut(s) | 532, 609 |
| Fsp4HI | GCNGC | 4 cut(s) | 144, 692, 708, 1146 |
| FspBI | CTAG | 3 cut(s) | 1248, 1283, 1326 |
| GluI | GCNGC | 4 cut(s) | 144, 692, 708, 1146 |
| GsaI | CCCAGC | 1 cut(s) | 778 |
| GsuI | CTGGAG | 2 cut(s) | 391, 707 |
| HaeIII | GGCC | 1 cut(s) | 1043 |
| Hin1II | CATG | 7 cut(s) | 173, 274, 355, 460, 536, 613, 1042 |
| HincII | GTYRAC | 1 cut(s) | 750 |
| HindII | GTYRAC | 1 cut(s) | 750 |
| HindIII | AAGCTT | 1 cut(s) | 1026 |
| HinfI | GANTC | 9 cut(s) | 56, 92, 542, 558, 619, 639, 794, 866, 977 |
| HphI | GGTGA | 3 cut(s) | 707, 763, 1346 |
| Hpy166II | GTNNAC | 7 cut(s) | 671, 750, 771, 883, 992, 1109, 1268 |
| Hpy188I | TCNGA | 7 cut(s) | 112, 222, 250, 824, 1159, 1201, 1261 |
| Hpy188III | TCNNGA | 9 cut(s) | 271, 521, 581, 598, 662, 863, 944, 974, 1119 |
| Hpy8I | GTNNAC | 7 cut(s) | 671, 750, 771, 883, 992, 1109, 1268 |
| HpyAV | CCTTC | 8 cut(s) | 429, 509, 572, 653, 692, 736, 916, 1248 |
| HpyCH4III | ACNGT | 4 cut(s) | 154, 719, 916, 999 |
| HpyCH4IV | ACGT | 2 cut(s) | 631, 1244 |
| HpyCH4V | TGCA | 9 cut(s) | 299, 335, 430, 671, 1075, 1172, 1208, 1220, 1256 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 784, 1013, 1067, 1169, 1178 |
| HpyF3I | CTNAG | 6 cut(s) | 347, 461, 481, 848, 1066, 1158 |
| HpySE526I | ACGT | 2 cut(s) | 631, 1244 |
| Hsp92II | CATG | 7 cut(s) | 173, 274, 355, 460, 536, 613, 1042 |
| Ksp22I | TGATCA | 1 cut(s) | 256 |
| Kzo9I | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| LmnI | GCTCC | 2 cut(s) | 151, 688 |
| Lsp1109I | GCAGC | 3 cut(s) | 130, 678, 1132 |
| LweI | GCATC | 7 cut(s) | 322, 574, 879, 1003, 1132, 1265, 1285 |
| MaeI | CTAG | 3 cut(s) | 1248, 1283, 1326 |
| MaeII | ACGT | 2 cut(s) | 631, 1244 |
| MaeIII | GTNAC | 3 cut(s) | 285, 365, 1321 |
| MalI | GATC | 4 cut(s) | 247, 258, 390, 1054 |
| MboI | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| MboII | GAAGA | 6 cut(s) | 175, 206, 241, 614, 948, 1289 |
| MfeI | CAATTG | 2 cut(s) | 189, 1209 |
| MhlI | GDGCHC | 5 cut(s) | 348, 532, 609, 673, 847 |
| MluCI | AATT | 9 cut(s) | 8, 189, 322, 492, 952, 1164, 1209, 1222, 1366 |
| MlyI | GAGTC | 4 cut(s) | 536, 552, 613, 633 |
| MmeI | TCCRAC | 1 cut(s) | 173 |
| MseI | TTAA | 3 cut(s) | 134, 312, 402 |
| MslI | CAYNNNNRTG | 2 cut(s) | 174, 294 |
| MspA1I | CMGCKG | 2 cut(s) | 262, 1373 |
| MunI | CAATTG | 2 cut(s) | 189, 1209 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 784, 1013, 1067, 1169, 1178 |
| NdeII | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| NlaIII | CATG | 7 cut(s) | 173, 274, 355, 460, 536, 613, 1042 |
| NlaIV | GGNNCC | 1 cut(s) | 1128 |
| NspI | RCATGY | 1 cut(s) | 173 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PfeI | GAWTC | 5 cut(s) | 56, 92, 794, 866, 977 |
| PkrI | GCNGC | 4 cut(s) | 145, 693, 709, 1147 |
| PleI | GAGTC | 4 cut(s) | 536, 552, 613, 633 |
| PpsI | GAGTC | 4 cut(s) | 536, 552, 613, 633 |
| Ppu21I | YACGTR | 1 cut(s) | 632 |
| PpuMI | RGGWCCY | 1 cut(s) | 1235 |
| Psp124BI | GAGCTC | 2 cut(s) | 532, 609 |
| Psp5II | RGGWCCY | 1 cut(s) | 1235 |
| PspFI | CCCAGC | 1 cut(s) | 774 |
| PspN4I | GGNNCC | 1 cut(s) | 1128 |
| PspPI | GGNCC | 2 cut(s) | 1093, 1235 |
| PspPPI | RGGWCCY | 1 cut(s) | 1235 |
| PstI | CTGCAG | 1 cut(s) | 1174 |
| PstNI | CAGNNNCTG | 2 cut(s) | 787, 1145 |
| RsaI | GTAC | 5 cut(s) | 210, 237, 630, 759, 1035 |
| RsaNI | GTAC | 5 cut(s) | 209, 236, 629, 758, 1034 |
| RseI | CAYNNNNRTG | 2 cut(s) | 174, 294 |
| SacI | GAGCTC | 2 cut(s) | 532, 609 |
| SaqAI | TTAA | 3 cut(s) | 134, 312, 402 |
| SatI | GCNGC | 4 cut(s) | 144, 692, 708, 1146 |
| Sau3AI | GATC | 4 cut(s) | 245, 256, 388, 1052 |
| Sau96I | GGNCC | 2 cut(s) | 1093, 1235 |
| ScaI | AGTACT | 1 cut(s) | 210 |
| SchI | GAGTC | 4 cut(s) | 536, 552, 613, 633 |
| SduI | GDGCHC | 5 cut(s) | 348, 532, 609, 673, 847 |
| SfaNI | GCATC | 7 cut(s) | 322, 574, 879, 1003, 1132, 1265, 1285 |
| SfcI | CTRYAG | 2 cut(s) | 995, 1170 |
| SinI | GGWCC | 2 cut(s) | 1093, 1235 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 174, 294 |
| SmlI | CTYRAG | 1 cut(s) | 500 |
| SmoI | CTYRAG | 1 cut(s) | 500 |
| Sse9I | AATT | 9 cut(s) | 8, 189, 322, 492, 952, 1164, 1209, 1222, 1366 |
| SsiI | CCGC | 3 cut(s) | 262, 707, 1373 |
| SspMI | CTAG | 3 cut(s) | 1248, 1283, 1326 |
| SstI | GAGCTC | 2 cut(s) | 532, 609 |
| TaaI | ACNGT | 4 cut(s) | 154, 719, 916, 999 |
| TaiI | ACGT | 2 cut(s) | 634, 1247 |
| TaqI | TCGA | 4 cut(s) | 161, 411, 435, 980 |
| TasI | AATT | 9 cut(s) | 8, 189, 322, 492, 952, 1164, 1209, 1222, 1366 |
| TatI | WGTACW | 3 cut(s) | 208, 235, 757 |
| TauI | GCSGC | 1 cut(s) | 710 |
| TfiI | GAWTC | 5 cut(s) | 56, 92, 794, 866, 977 |
| Tru1I | TTAA | 3 cut(s) | 134, 312, 402 |
| Tru9I | TTAA | 3 cut(s) | 134, 312, 402 |
| TscAI | CASTG | 6 cut(s) | 489, 852, 921, 1004, 1116, 1309 |
| TseI | GCWGC | 3 cut(s) | 143, 691, 1145 |
| TspDTI | ATGAA | 7 cut(s) | 17, 35, 54, 129, 171, 755, 807 |
| TspRI | CASTG | 6 cut(s) | 489, 852, 921, 1004, 1116, 1309 |
| VneI | GTGCAC | 1 cut(s) | 669 |
| VpaK11BI | GGWCC | 2 cut(s) | 1093, 1235 |
| XapI | RAATTY | 4 cut(s) | 8, 492, 1222, 1366 |
| XceI | RCATGY | 1 cut(s) | 173 |
| XmiI | GTMKAC | 1 cut(s) | 882 |
| XspI | CTAG | 3 cut(s) | 1248, 1283, 1326 |
| ZrmI | AGTACT | 1 cut(s) | 210 |