NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 413 |
| AccB1I | GGYRCC | 1 cut(s) | 868 |
| AccI | GTMKAC | 1 cut(s) | 660 |
| AciI | CCGC | 2 cut(s) | 178, 479 |
| AclWI | GGATC | 2 cut(s) | 169, 290 |
| AcsI | RAATTY | 1 cut(s) | 931 |
| AcuI | CTGAAG | 6 cut(s) | 57, 401, 536, 580, 768, 1056 |
| AfaI | GTAC | 4 cut(s) | 126, 153, 801, 1112 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 553 |
| AgsI | TTSAA | 6 cut(s) | 110, 118, 743, 791, 984, 1079 |
| AjnI | CCWGG | 1 cut(s) | 30 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 140, 172 |
| AluBI | AGCT | 6 cut(s) | 18, 313, 383, 550, 794, 812 |
| AluI | AGCT | 6 cut(s) | 18, 313, 383, 550, 794, 812 |
| Alw21I | GWGCWC | 2 cut(s) | 264, 445 |
| Alw26I | GTCTC | 3 cut(s) | 76, 161, 293 |
| Alw44I | GTGCAC | 1 cut(s) | 441 |
| AlwI | GGATC | 2 cut(s) | 169, 290 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 170, 559 |
| Ama87I | CYCGRG | 1 cut(s) | 859 |
| ApaLI | GTGCAC | 1 cut(s) | 441 |
| ApeKI | GCWGC | 4 cut(s) | 59, 463, 667, 733 |
| ApoI | RAATTY | 1 cut(s) | 931 |
| AspLEI | GCGC | 1 cut(s) | 73 |
| AspS9I | GGNCC | 1 cut(s) | 835 |
| AsuHPI | GGTGA | 2 cut(s) | 535, 1106 |
| AvaI | CYCGRG | 1 cut(s) | 859 |
| AvaII | GGWCC | 1 cut(s) | 835 |
| BaeGI | GKGCMC | 1 cut(s) | 445 |
| BanI | GGYRCC | 1 cut(s) | 868 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 783, 815 |
| BauI | CACGAG | 1 cut(s) | 402 |
| Bbv12I | GWGCWC | 2 cut(s) | 264, 445 |
| BbvI | GCAGC | 4 cut(s) | 46, 450, 654, 745 |
| BccI | CCATC | 4 cut(s) | 35, 86, 307, 656 |
| BciT130I | CCWGG | 1 cut(s) | 32 |
| BclI | TGATCA | 2 cut(s) | 172, 585 |
| BcoDI | GTCTC | 3 cut(s) | 76, 161, 293 |
| BfaI | CTAG | 2 cut(s) | 990, 1043 |
| BfmI | CTRYAG | 1 cut(s) | 761 |
| BfuAI | ACCTGC | 1 cut(s) | 413 |
| BisI | GCNGC | 5 cut(s) | 60, 464, 480, 668, 734 |
| BlpI | GCTNAGC | 1 cut(s) | 263 |
| BlsI | GCNGC | 5 cut(s) | 61, 465, 481, 669, 735 |
| BmcAI | AGTACT | 2 cut(s) | 126, 1112 |
| Bme1390I | CCNGG | 1 cut(s) | 32 |
| Bme18I | GGWCC | 1 cut(s) | 835 |
| BmeT110I | CYCGRG | 1 cut(s) | 859 |
| BmgT120I | GGNCC | 1 cut(s) | 835 |
| BmiI | GGNNCC | 1 cut(s) | 870 |
| BmrFI | CCNGG | 1 cut(s) | 32 |
| BmrI | ACTGGG | 2 cut(s) | 467, 632 |
| BmsI | GCATC | 5 cut(s) | 238, 745, 769, 1007, 1045 |
| BmuI | ACTGGG | 2 cut(s) | 467, 632 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 263 |
| Bsa29I | ATCGAT | 1 cut(s) | 342 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 553 |
| Bse1I | ACTGG | 3 cut(s) | 462, 627, 803 |
| Bse3DI | GCAATG | 2 cut(s) | 98, 258 |
| BseBI | CCWGG | 1 cut(s) | 32 |
| BseCI | ATCGAT | 1 cut(s) | 342 |
| BseGI | GGATG | 2 cut(s) | 568, 633 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 553 |
| BseMI | GCAATG | 2 cut(s) | 98, 258 |
| BseMII | CTCAG | 1 cut(s) | 277 |
| BseNI | ACTGG | 3 cut(s) | 462, 627, 803 |
| BseRI | GAGGAG | 2 cut(s) | 287, 290 |
| BseSI | GKGCMC | 1 cut(s) | 445 |
| BseXI | GCAGC | 4 cut(s) | 46, 450, 654, 745 |
| BseYI | CCCAGC | 1 cut(s) | 546 |
| BsgI | GTGCAG | 1 cut(s) | 513 |
| BshNI | GGYRCC | 1 cut(s) | 868 |
| BshVI | ATCGAT | 1 cut(s) | 342 |
| BsiHKAI | GWGCWC | 2 cut(s) | 264, 445 |
| BsiHKCI | CYCGRG | 1 cut(s) | 859 |
| BslFI | GGGAC | 2 cut(s) | 841, 876 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 553 |
| BsmAI | GTCTC | 3 cut(s) | 76, 161, 293 |
| BsmFI | GGGAC | 2 cut(s) | 841, 876 |
| BsoBI | CYCGRG | 1 cut(s) | 859 |
| Bsp1286I | GDGCHC | 2 cut(s) | 264, 445 |
| Bsp143I | GATC | 4 cut(s) | 161, 172, 295, 585 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 263 |
| BspACI | CCGC | 2 cut(s) | 178, 479 |
| BspCNI | CTCAG | 1 cut(s) | 276 |
| BspDI | ATCGAT | 1 cut(s) | 342 |
| BspHI | TCATGA | 1 cut(s) | 186 |
| BspLI | GGNNCC | 1 cut(s) | 870 |
| BspMI | ACCTGC | 1 cut(s) | 413 |
| BspPI | GGATC | 2 cut(s) | 169, 290 |
| BspT107I | GGYRCC | 1 cut(s) | 868 |
| BsrDI | GCAATG | 2 cut(s) | 98, 258 |
| BsrI | ACTGG | 3 cut(s) | 462, 627, 803 |
| BssMI | GATC | 4 cut(s) | 161, 172, 295, 585 |
| BssNAI | GTATAC | 1 cut(s) | 661 |
| BssSI | CACGAG | 1 cut(s) | 402 |
| Bst1107I | GTATAC | 1 cut(s) | 661 |
| Bst2BI | CACGAG | 1 cut(s) | 402 |
| Bst2UI | CCWGG | 1 cut(s) | 32 |
| Bst4CI | ACNGT | 4 cut(s) | 491, 517, 694, 765 |
| Bst6I | CTCTTC | 2 cut(s) | 74, 403 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 779 |
| BstC8I | GCNNGC | 2 cut(s) | 180, 441 |
| BstDEI | CTNAG | 3 cut(s) | 263, 808, 1119 |
| BstF5I | GGATG | 2 cut(s) | 568, 633 |
| BstHHI | GCGC | 1 cut(s) | 73 |
| BstKTI | GATC | 4 cut(s) | 164, 175, 298, 588 |
| BstMAI | GTCTC | 3 cut(s) | 76, 161, 293 |
| BstMBI | GATC | 4 cut(s) | 161, 172, 295, 585 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 68, 556, 779 |
| BstNI | CCWGG | 1 cut(s) | 32 |
| BstNSI | RCATGY | 1 cut(s) | 89 |
| BstSCI | CCNGG | 1 cut(s) | 30 |
| BstSFI | CTRYAG | 1 cut(s) | 761 |
| BstSLI | GKGCMC | 1 cut(s) | 445 |
| BstV1I | GCAGC | 4 cut(s) | 46, 450, 654, 745 |
| BstZ17I | GTATAC | 1 cut(s) | 661 |
| Bsu15I | ATCGAT | 1 cut(s) | 342 |
| BsuTUI | ATCGAT | 1 cut(s) | 342 |
| BtsCI | GGATG | 2 cut(s) | 568, 633 |
| BtsIMutI | CAGTG | 5 cut(s) | 496, 699, 770, 951, 1062 |
| BveI | ACCTGC | 1 cut(s) | 413 |
| Cac8I | GCNNGC | 2 cut(s) | 180, 441 |
| CaiI | CAGNNNCTG | 2 cut(s) | 170, 559 |
| CciI | TCATGA | 1 cut(s) | 186 |
| CfoI | GCGC | 1 cut(s) | 73 |
| Cfr13I | GGNCC | 1 cut(s) | 835 |
| ClaI | ATCGAT | 1 cut(s) | 342 |
| CseI | GACGC | 1 cut(s) | 930 |
| Csp6I | GTAC | 4 cut(s) | 125, 152, 800, 1111 |
| CviAII | CATG | 5 cut(s) | 86, 187, 268, 386, 589 |
| CviQI | GTAC | 4 cut(s) | 125, 152, 800, 1111 |
| DdeI | CTNAG | 3 cut(s) | 263, 808, 1119 |
| DpnI | GATC | 4 cut(s) | 163, 174, 297, 587 |
| DpnII | GATC | 4 cut(s) | 161, 172, 295, 585 |
| Eam1104I | CTCTTC | 2 cut(s) | 74, 403 |
| EarI | CTCTTC | 2 cut(s) | 74, 403 |
| Eco47I | GGWCC | 1 cut(s) | 835 |
| Eco57I | CTGAAG | 6 cut(s) | 57, 401, 536, 580, 768, 1056 |
| Eco88I | CYCGRG | 1 cut(s) | 859 |
| EcoRII | CCWGG | 1 cut(s) | 30 |
| EcoT22I | ATGCAT | 1 cut(s) | 1000 |
| FaeI | CATG | 5 cut(s) | 89, 190, 271, 389, 592 |
| FaqI | GGGAC | 2 cut(s) | 841, 876 |
| FatI | CATG | 5 cut(s) | 85, 186, 267, 385, 588 |
| FauI | CCCGC | 1 cut(s) | 171 |
| FbaI | TGATCA | 2 cut(s) | 172, 585 |
| FblI | GTMKAC | 1 cut(s) | 660 |
| Fnu4HI | GCNGC | 5 cut(s) | 60, 464, 480, 668, 734 |
| FokI | GGATG | 2 cut(s) | 575, 640 |
| Fsp4HI | GCNGC | 5 cut(s) | 60, 464, 480, 668, 734 |
| FspBI | CTAG | 2 cut(s) | 990, 1043 |
| GlaI | GCGC | 1 cut(s) | 72 |
| GluI | GCNGC | 5 cut(s) | 60, 464, 480, 668, 734 |
| GsaI | CCCAGC | 1 cut(s) | 550 |
| HgaI | GACGC | 1 cut(s) | 930 |
| HhaI | GCGC | 1 cut(s) | 73 |
| Hin1II | CATG | 5 cut(s) | 89, 190, 271, 389, 592 |
| Hin6I | GCGC | 1 cut(s) | 71 |
| HinP1I | GCGC | 1 cut(s) | 71 |
| HincII | GTYRAC | 2 cut(s) | 522, 851 |
| HindII | GTYRAC | 2 cut(s) | 522, 851 |
| HindIII | AAGCTT | 1 cut(s) | 792 |
| HinfI | GANTC | 6 cut(s) | 395, 411, 566, 673, 743, 1010 |
| HpaI | GTTAAC | 1 cut(s) | 522 |
| HphI | GGTGA | 2 cut(s) | 535, 1106 |
| Hpy166II | GTNNAC | 6 cut(s) | 443, 522, 543, 661, 758, 851 |
| Hpy188I | TCNGA | 5 cut(s) | 138, 166, 599, 1003, 1015 |
| Hpy188III | TCNNGA | 5 cut(s) | 187, 434, 638, 740, 861 |
| Hpy8I | GTNNAC | 6 cut(s) | 443, 522, 543, 661, 758, 851 |
| HpyAV | CCTTC | 3 cut(s) | 336, 425, 694 |
| HpyCH4III | ACNGT | 4 cut(s) | 491, 517, 694, 765 |
| HpyCH4IV | ACGT | 2 cut(s) | 924, 986 |
| HpyCH4V | TGCA | 9 cut(s) | 215, 251, 337, 443, 530, 817, 917, 929, 998 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 68, 556, 779 |
| HpyF3I | CTNAG | 3 cut(s) | 263, 808, 1119 |
| HpySE526I | ACGT | 2 cut(s) | 924, 986 |
| Hsp92II | CATG | 5 cut(s) | 89, 190, 271, 389, 592 |
| HspAI | GCGC | 1 cut(s) | 71 |
| Ksp22I | TGATCA | 2 cut(s) | 172, 585 |
| KspAI | GTTAAC | 1 cut(s) | 522 |
| Kzo9I | GATC | 4 cut(s) | 161, 172, 295, 585 |
| LmnI | GCTCC | 1 cut(s) | 67 |
| Lsp1109I | GCAGC | 4 cut(s) | 46, 450, 654, 745 |
| LweI | GCATC | 5 cut(s) | 238, 745, 769, 1007, 1045 |
| MaeI | CTAG | 2 cut(s) | 990, 1043 |
| MaeII | ACGT | 2 cut(s) | 924, 986 |
| MaeIII | GTNAC | 1 cut(s) | 201 |
| MalI | GATC | 4 cut(s) | 163, 174, 297, 587 |
| MboI | GATC | 4 cut(s) | 161, 172, 295, 585 |
| MboII | GAAGA | 7 cut(s) | 50, 91, 122, 157, 390, 714, 1049 |
| MfeI | CAATTG | 3 cut(s) | 105, 155, 918 |
| MhlI | GDGCHC | 2 cut(s) | 264, 445 |
| MluCI | AATT | 6 cut(s) | 105, 155, 238, 718, 918, 931 |
| MlyI | GAGTC | 2 cut(s) | 389, 405 |
| MmeI | TCCRAC | 2 cut(s) | 89, 952 |
| MnlI | CCTC | 7 cut(s) | 265, 268, 271, 361, 676, 818, 968 |
| Mph1103I | ATGCAT | 1 cut(s) | 1000 |
| MseI | TTAA | 5 cut(s) | 228, 309, 375, 521, 1083 |
| MslI | CAYNNNNRTG | 2 cut(s) | 90, 210 |
| MspA1I | CMGCKG | 1 cut(s) | 178 |
| MspR9I | CCNGG | 1 cut(s) | 32 |
| MunI | CAATTG | 3 cut(s) | 105, 155, 918 |
| MvaI | CCWGG | 1 cut(s) | 32 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 68, 556, 779 |
| NdeII | GATC | 4 cut(s) | 161, 172, 295, 585 |
| NlaIII | CATG | 5 cut(s) | 89, 190, 271, 389, 592 |
| NlaIV | GGNNCC | 1 cut(s) | 870 |
| NsiI | ATGCAT | 1 cut(s) | 1000 |
| NspI | RCATGY | 1 cut(s) | 89 |
| PagI | TCATGA | 1 cut(s) | 186 |
| PfeI | GAWTC | 4 cut(s) | 566, 673, 743, 1010 |
| PkrI | GCNGC | 5 cut(s) | 61, 465, 481, 669, 735 |
| PleI | GAGTC | 2 cut(s) | 389, 405 |
| PpsI | GAGTC | 2 cut(s) | 389, 405 |
| Psp6I | CCWGG | 1 cut(s) | 30 |
| PspFI | CCCAGC | 1 cut(s) | 546 |
| PspGI | CCWGG | 1 cut(s) | 30 |
| PspN4I | GGNNCC | 1 cut(s) | 870 |
| PspPI | GGNCC | 1 cut(s) | 835 |
| PstNI | CAGNNNCTG | 2 cut(s) | 170, 559 |
| RsaI | GTAC | 4 cut(s) | 126, 153, 801, 1112 |
| RsaNI | GTAC | 4 cut(s) | 125, 152, 800, 1111 |
| RseI | CAYNNNNRTG | 2 cut(s) | 90, 210 |
| SaqAI | TTAA | 5 cut(s) | 228, 309, 375, 521, 1083 |
| SatI | GCNGC | 5 cut(s) | 60, 464, 480, 668, 734 |
| Sau3AI | GATC | 4 cut(s) | 161, 172, 295, 585 |
| Sau96I | GGNCC | 1 cut(s) | 835 |
| ScaI | AGTACT | 2 cut(s) | 126, 1112 |
| SchI | GAGTC | 2 cut(s) | 389, 405 |
| ScrFI | CCNGG | 1 cut(s) | 32 |
| SduI | GDGCHC | 2 cut(s) | 264, 445 |
| SfaNI | GCATC | 5 cut(s) | 238, 745, 769, 1007, 1045 |
| SfcI | CTRYAG | 1 cut(s) | 761 |
| SinI | GGWCC | 1 cut(s) | 835 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 90, 210 |
| Sse9I | AATT | 6 cut(s) | 105, 155, 238, 718, 918, 931 |
| SsiI | CCGC | 2 cut(s) | 178, 479 |
| SspMI | CTAG | 2 cut(s) | 990, 1043 |
| StyD4I | CCNGG | 1 cut(s) | 30 |
| TaaI | ACNGT | 4 cut(s) | 491, 517, 694, 765 |
| TaiI | ACGT | 2 cut(s) | 927, 989 |
| TaqI | TCGA | 3 cut(s) | 77, 318, 342 |
| TasI | AATT | 6 cut(s) | 105, 155, 238, 718, 918, 931 |
| TatI | WGTACW | 3 cut(s) | 124, 151, 1110 |
| TauI | GCSGC | 1 cut(s) | 482 |
| TfiI | GAWTC | 4 cut(s) | 566, 673, 743, 1010 |
| Tru1I | TTAA | 5 cut(s) | 228, 309, 375, 521, 1083 |
| Tru9I | TTAA | 5 cut(s) | 228, 309, 375, 521, 1083 |
| TscAI | CASTG | 5 cut(s) | 496, 699, 770, 951, 1069 |
| TseI | GCWGC | 4 cut(s) | 59, 463, 667, 733 |
| TspDTI | ATGAA | 3 cut(s) | 87, 352, 579 |
| TspRI | CASTG | 5 cut(s) | 496, 699, 770, 951, 1069 |
| VneI | GTGCAC | 1 cut(s) | 441 |
| VpaK11BI | GGWCC | 1 cut(s) | 835 |
| XapI | RAATTY | 1 cut(s) | 931 |
| XceI | RCATGY | 1 cut(s) | 89 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 38 |
| XmiI | GTMKAC | 1 cut(s) | 660 |
| XspI | CTAG | 2 cut(s) | 990, 1043 |
| ZrmI | AGTACT | 2 cut(s) | 126, 1112 |
| Zsp2I | ATGCAT | 1 cut(s) | 1000 |