NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 112 |
| AciI | CCGC | 2 cut(s) | 241, 632 |
| AclWI | GGATC | 2 cut(s) | 232, 410 |
| AcsI | RAATTY | 1 cut(s) | 519 |
| AcuI | CTGAAG | 1 cut(s) | 576 |
| AfaI | GTAC | 6 cut(s) | 89, 189, 216, 453, 684, 747 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 719 |
| AgsI | TTSAA | 5 cut(s) | 85, 173, 181, 194, 551 |
| AluBI | AGCT | 5 cut(s) | 44, 343, 433, 557, 616 |
| AluI | AGCT | 5 cut(s) | 44, 343, 433, 557, 616 |
| Alw21I | GWGCWC | 1 cut(s) | 327 |
| Alw26I | GTCTC | 4 cut(s) | 139, 224, 354, 486 |
| AlwI | GGATC | 2 cut(s) | 232, 410 |
| ApeKI | GCWGC | 4 cut(s) | 94, 122, 340, 616 |
| ApoI | RAATTY | 1 cut(s) | 519 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 11 |
| AspLEI | GCGC | 1 cut(s) | 136 |
| AsuHPI | GGTGA | 1 cut(s) | 688 |
| Bbv12I | GWGCWC | 1 cut(s) | 327 |
| BbvI | GCAGC | 4 cut(s) | 81, 109, 352, 603 |
| BccI | CCATC | 1 cut(s) | 149 |
| BclI | TGATCA | 1 cut(s) | 235 |
| BcoDI | GTCTC | 4 cut(s) | 139, 224, 354, 486 |
| BisI | GCNGC | 5 cut(s) | 95, 123, 341, 617, 633 |
| BlpI | GCTNAGC | 1 cut(s) | 326 |
| BlsI | GCNGC | 5 cut(s) | 96, 124, 342, 618, 634 |
| BmcAI | AGTACT | 2 cut(s) | 89, 189 |
| BmsI | GCATC | 1 cut(s) | 301 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 391, 423 |
| BpmI | CTGGAG | 1 cut(s) | 632 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 326 |
| BpuEI | CTTGAG | 1 cut(s) | 548 |
| Bsa29I | ATCGAT | 1 cut(s) | 462 |
| BsaJI | CCNNGG | 1 cut(s) | 737 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 719 |
| Bse1I | ACTGG | 3 cut(s) | 95, 506, 615 |
| Bse3DI | GCAATG | 2 cut(s) | 161, 321 |
| BseCI | ATCGAT | 1 cut(s) | 462 |
| BseDI | CCNNGG | 1 cut(s) | 737 |
| BseGI | GGATG | 1 cut(s) | 709 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 719 |
| BseMI | GCAATG | 2 cut(s) | 161, 321 |
| BseMII | CTCAG | 4 cut(s) | 36, 130, 340, 522 |
| BseNI | ACTGG | 3 cut(s) | 95, 506, 615 |
| BseRI | GAGGAG | 1 cut(s) | 350 |
| BseXI | GCAGC | 4 cut(s) | 81, 109, 352, 603 |
| BshVI | ATCGAT | 1 cut(s) | 462 |
| BsiHKAI | GWGCWC | 1 cut(s) | 327 |
| BsiSI | CCGG | 1 cut(s) | 98 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 719 |
| BsmAI | GTCTC | 4 cut(s) | 139, 224, 354, 486 |
| Bsp1286I | GDGCHC | 1 cut(s) | 327 |
| Bsp1407I | TGTACA | 1 cut(s) | 682 |
| Bsp143I | GATC | 3 cut(s) | 224, 235, 415 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 326 |
| BspACI | CCGC | 2 cut(s) | 241, 632 |
| BspCNI | CTCAG | 4 cut(s) | 37, 129, 339, 521 |
| BspDI | ATCGAT | 1 cut(s) | 462 |
| BspHI | TCATGA | 1 cut(s) | 249 |
| BspPI | GGATC | 2 cut(s) | 232, 410 |
| BsrDI | GCAATG | 2 cut(s) | 161, 321 |
| BsrGI | TGTACA | 1 cut(s) | 682 |
| BsrI | ACTGG | 3 cut(s) | 95, 506, 615 |
| BssECI | CCNNGG | 1 cut(s) | 737 |
| BssMI | GATC | 3 cut(s) | 224, 235, 415 |
| BssT1I | CCWWGG | 1 cut(s) | 737 |
| Bst4CI | ACNGT | 2 cut(s) | 644, 670 |
| Bst6I | CTCTTC | 2 cut(s) | 137, 577 |
| BstAUI | TGTACA | 1 cut(s) | 682 |
| BstC8I | GCNNGC | 1 cut(s) | 243 |
| BstDEI | CTNAG | 5 cut(s) | 45, 116, 326, 488, 508 |
| BstF5I | GGATG | 1 cut(s) | 709 |
| BstHHI | GCGC | 1 cut(s) | 136 |
| BstKTI | GATC | 3 cut(s) | 227, 238, 418 |
| BstMAI | GTCTC | 4 cut(s) | 139, 224, 354, 486 |
| BstMBI | GATC | 3 cut(s) | 224, 235, 415 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 131 |
| BstNSI | RCATGY | 1 cut(s) | 152 |
| BstV1I | GCAGC | 4 cut(s) | 81, 109, 352, 603 |
| Bsu15I | ATCGAT | 1 cut(s) | 462 |
| BsuTUI | ATCGAT | 1 cut(s) | 462 |
| BtsCI | GGATG | 1 cut(s) | 709 |
| BtsIMutI | CAGTG | 2 cut(s) | 516, 649 |
| Cac8I | GCNNGC | 1 cut(s) | 243 |
| CciI | TCATGA | 1 cut(s) | 249 |
| CfoI | GCGC | 1 cut(s) | 136 |
| ClaI | ATCGAT | 1 cut(s) | 462 |
| Csp6I | GTAC | 6 cut(s) | 88, 188, 215, 452, 683, 746 |
| CviAII | CATG | 5 cut(s) | 149, 250, 331, 484, 560 |
| CviQI | GTAC | 6 cut(s) | 88, 188, 215, 452, 683, 746 |
| DdeI | CTNAG | 5 cut(s) | 45, 116, 326, 488, 508 |
| DpnI | GATC | 3 cut(s) | 226, 237, 417 |
| DpnII | GATC | 3 cut(s) | 224, 235, 415 |
| Eam1104I | CTCTTC | 2 cut(s) | 137, 577 |
| EarI | CTCTTC | 2 cut(s) | 137, 577 |
| Eco130I | CCWWGG | 1 cut(s) | 737 |
| Eco57I | CTGAAG | 1 cut(s) | 576 |
| EcoRI | GAATTC | 1 cut(s) | 519 |
| EcoT14I | CCWWGG | 1 cut(s) | 737 |
| ErhI | CCWWGG | 1 cut(s) | 737 |
| FaeI | CATG | 5 cut(s) | 152, 253, 334, 487, 563 |
| FatI | CATG | 5 cut(s) | 148, 249, 330, 483, 559 |
| FauI | CCCGC | 1 cut(s) | 234 |
| FbaI | TGATCA | 1 cut(s) | 235 |
| FblI | GTMKAC | 1 cut(s) | 112 |
| Fnu4HI | GCNGC | 5 cut(s) | 95, 123, 341, 617, 633 |
| FokI | GGATG | 1 cut(s) | 696 |
| Fsp4HI | GCNGC | 5 cut(s) | 95, 123, 341, 617, 633 |
| GlaI | GCGC | 1 cut(s) | 135 |
| GluI | GCNGC | 5 cut(s) | 95, 123, 341, 617, 633 |
| GsuI | CTGGAG | 1 cut(s) | 632 |
| HapII | CCGG | 1 cut(s) | 98 |
| HhaI | GCGC | 1 cut(s) | 136 |
| Hin1II | CATG | 5 cut(s) | 152, 253, 334, 487, 563 |
| Hin6I | GCGC | 1 cut(s) | 134 |
| HinP1I | GCGC | 1 cut(s) | 134 |
| HincII | GTYRAC | 1 cut(s) | 675 |
| HindII | GTYRAC | 1 cut(s) | 675 |
| HinfI | GANTC | 4 cut(s) | 114, 569, 586, 703 |
| HpaII | CCGG | 1 cut(s) | 98 |
| HphI | GGTGA | 1 cut(s) | 688 |
| Hpy166II | GTNNAC | 3 cut(s) | 113, 675, 696 |
| Hpy188I | TCNGA | 2 cut(s) | 201, 229 |
| Hpy188III | TCNNGA | 2 cut(s) | 250, 548 |
| Hpy8I | GTNNAC | 3 cut(s) | 113, 675, 696 |
| HpyAV | CCTTC | 5 cut(s) | 95, 99, 456, 600, 617 |
| HpyCH4III | ACNGT | 2 cut(s) | 644, 670 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 20, 278, 314, 457 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 131 |
| HpyF3I | CTNAG | 5 cut(s) | 45, 116, 326, 488, 508 |
| Hsp92II | CATG | 5 cut(s) | 152, 253, 334, 487, 563 |
| HspAI | GCGC | 1 cut(s) | 134 |
| Ksp22I | TGATCA | 1 cut(s) | 235 |
| Kzo9I | GATC | 3 cut(s) | 224, 235, 415 |
| LmnI | GCTCC | 3 cut(s) | 130, 337, 613 |
| Lsp1109I | GCAGC | 4 cut(s) | 81, 109, 352, 603 |
| LweI | GCATC | 1 cut(s) | 301 |
| MaeIII | GTNAC | 1 cut(s) | 264 |
| MalI | GATC | 3 cut(s) | 226, 237, 417 |
| MboI | GATC | 3 cut(s) | 224, 235, 415 |
| MboII | GAAGA | 6 cut(s) | 154, 185, 220, 368, 564, 698 |
| MfeI | CAATTG | 1 cut(s) | 168 |
| MhlI | GDGCHC | 1 cut(s) | 327 |
| MluCI | AATT | 4 cut(s) | 168, 301, 519, 754 |
| MlyI | GAGTC | 3 cut(s) | 108, 563, 580 |
| MmeI | TCCRAC | 1 cut(s) | 152 |
| MnlI | CCTC | 4 cut(s) | 328, 393, 535, 723 |
| MroXI | GAANNNNTTC | 1 cut(s) | 11 |
| MseI | TTAA | 5 cut(s) | 291, 354, 429, 582, 757 |
| MslI | CAYNNNNRTG | 2 cut(s) | 153, 273 |
| MspA1I | CMGCKG | 1 cut(s) | 241 |
| MspI | CCGG | 1 cut(s) | 98 |
| MunI | CAATTG | 1 cut(s) | 168 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 131 |
| NdeII | GATC | 3 cut(s) | 224, 235, 415 |
| NlaIII | CATG | 5 cut(s) | 152, 253, 334, 487, 563 |
| NspI | RCATGY | 1 cut(s) | 152 |
| PagI | TCATGA | 1 cut(s) | 249 |
| PdmI | GAANNNNTTC | 1 cut(s) | 11 |
| PfeI | GAWTC | 1 cut(s) | 703 |
| PkrI | GCNGC | 5 cut(s) | 96, 124, 342, 618, 634 |
| PleI | GAGTC | 3 cut(s) | 108, 563, 580 |
| PpsI | GAGTC | 3 cut(s) | 108, 563, 580 |
| RsaI | GTAC | 6 cut(s) | 89, 189, 216, 453, 684, 747 |
| RsaNI | GTAC | 6 cut(s) | 88, 188, 215, 452, 683, 746 |
| RseI | CAYNNNNRTG | 2 cut(s) | 153, 273 |
| SaqAI | TTAA | 5 cut(s) | 291, 354, 429, 582, 757 |
| SatI | GCNGC | 5 cut(s) | 95, 123, 341, 617, 633 |
| Sau3AI | GATC | 3 cut(s) | 224, 235, 415 |
| ScaI | AGTACT | 2 cut(s) | 89, 189 |
| SchI | GAGTC | 3 cut(s) | 108, 563, 580 |
| SduI | GDGCHC | 1 cut(s) | 327 |
| SetI | ASST | 7 cut(s) | 12, 46, 345, 435, 559, 618, 654 |
| SfaNI | GCATC | 1 cut(s) | 301 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 153, 273 |
| SmlI | CTYRAG | 1 cut(s) | 527 |
| SmoI | CTYRAG | 1 cut(s) | 527 |
| Sse9I | AATT | 4 cut(s) | 168, 301, 519, 754 |
| SsiI | CCGC | 2 cut(s) | 241, 632 |
| StyI | CCWWGG | 1 cut(s) | 737 |
| TaaI | ACNGT | 2 cut(s) | 644, 670 |
| TaqI | TCGA | 4 cut(s) | 54, 140, 438, 462 |
| TasI | AATT | 4 cut(s) | 168, 301, 519, 754 |
| TatI | WGTACW | 6 cut(s) | 87, 187, 214, 451, 682, 745 |
| TauI | GCSGC | 1 cut(s) | 635 |
| TfiI | GAWTC | 1 cut(s) | 703 |
| Tru1I | TTAA | 5 cut(s) | 291, 354, 429, 582, 757 |
| Tru9I | TTAA | 5 cut(s) | 291, 354, 429, 582, 757 |
| TscAI | CASTG | 2 cut(s) | 516, 649 |
| TseI | GCWGC | 4 cut(s) | 94, 122, 340, 616 |
| TspDTI | ATGAA | 4 cut(s) | 150, 377, 472, 698 |
| TspRI | CASTG | 2 cut(s) | 516, 649 |
| XapI | RAATTY | 1 cut(s) | 519 |
| XceI | RCATGY | 1 cut(s) | 152 |
| XmiI | GTMKAC | 1 cut(s) | 112 |
| XmnI | GAANNNNTTC | 1 cut(s) | 11 |
| ZrmI | AGTACT | 2 cut(s) | 89, 189 |