NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 218 |
| AccB1I | GGYRCC | 2 cut(s) | 424, 709 |
| AccI | GTMKAC | 1 cut(s) | 465 |
| AciI | CCGC | 2 cut(s) | 284, 1007 |
| AclWI | GGATC | 1 cut(s) | 41 |
| AcsI | RAATTY | 3 cut(s) | 150, 805, 1000 |
| AcuI | CTGAAG | 6 cut(s) | 206, 341, 385, 497, 585, 930 |
| AfaI | GTAC | 3 cut(s) | 336, 618, 986 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 301, 358, 742 |
| AgsI | TTSAA | 5 cut(s) | 182, 560, 608, 858, 953 |
| AluBI | AGCT | 8 cut(s) | 64, 188, 268, 355, 611, 653, 731, 992 |
| AluI | AGCT | 8 cut(s) | 64, 188, 268, 355, 611, 653, 731, 992 |
| Alw21I | GWGCWC | 3 cut(s) | 17, 190, 250 |
| Alw26I | GTCTC | 1 cut(s) | 117 |
| Alw44I | GTGCAC | 1 cut(s) | 246 |
| AlwI | GGATC | 1 cut(s) | 41 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 364 |
| Ama87I | CYCGRG | 1 cut(s) | 700 |
| AoxI | GGCC | 1 cut(s) | 624 |
| ApaLI | GTGCAC | 1 cut(s) | 246 |
| ApeKI | GCWGC | 3 cut(s) | 268, 472, 728 |
| ApoI | RAATTY | 3 cut(s) | 150, 805, 1000 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 509 |
| AspS9I | GGNCC | 2 cut(s) | 676, 851 |
| AsuHPI | GGTGA | 3 cut(s) | 284, 340, 980 |
| AvaI | CYCGRG | 1 cut(s) | 700 |
| AvaII | GGWCC | 2 cut(s) | 676, 851 |
| BaeGI | GKGCMC | 2 cut(s) | 250, 427 |
| BanI | GGYRCC | 2 cut(s) | 424, 709 |
| BanII | GRGCYC | 1 cut(s) | 190 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 600, 632 |
| BauI | CACGAG | 1 cut(s) | 207 |
| Bbv12I | GWGCWC | 3 cut(s) | 17, 190, 250 |
| BbvI | GCAGC | 3 cut(s) | 255, 459, 740 |
| BccI | CCATC | 2 cut(s) | 58, 461 |
| BcoDI | GTCTC | 1 cut(s) | 117 |
| BfaI | CTAG | 3 cut(s) | 864, 917, 960 |
| BfmI | CTRYAG | 2 cut(s) | 578, 753 |
| BfuAI | ACCTGC | 1 cut(s) | 218 |
| BisI | GCNGC | 4 cut(s) | 269, 285, 473, 729 |
| BlpI | GCTNAGC | 1 cut(s) | 16 |
| BlsI | GCNGC | 4 cut(s) | 270, 286, 474, 730 |
| BmcAI | AGTACT | 1 cut(s) | 986 |
| Bme18I | GGWCC | 2 cut(s) | 676, 851 |
| BmeT110I | CYCGRG | 1 cut(s) | 700 |
| BmgT120I | GGNCC | 2 cut(s) | 676, 851 |
| BmiI | GGNNCC | 2 cut(s) | 426, 711 |
| BmsI | GCATC | 4 cut(s) | 586, 715, 881, 919 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 22, 54 |
| BpmI | CTGGAG | 1 cut(s) | 284 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 16 |
| BpuEI | CTTGAG | 1 cut(s) | 179 |
| Bsa29I | ATCGAT | 1 cut(s) | 93 |
| BsaJI | CCNNGG | 2 cut(s) | 295, 428 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 301, 358, 742 |
| Bse1I | ACTGG | 2 cut(s) | 137, 267 |
| Bse3DI | GCAATG | 2 cut(s) | 11, 798 |
| BseCI | ATCGAT | 1 cut(s) | 93 |
| BseDI | CCNNGG | 2 cut(s) | 295, 428 |
| BseGI | GGATG | 2 cut(s) | 373, 438 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 301, 358, 742 |
| BseMI | GCAATG | 2 cut(s) | 11, 798 |
| BseMII | CTCAG | 3 cut(s) | 30, 153, 732 |
| BseNI | ACTGG | 2 cut(s) | 137, 267 |
| BseRI | GAGGAG | 2 cut(s) | 40, 43 |
| BseSI | GKGCMC | 2 cut(s) | 250, 427 |
| BseXI | GCAGC | 3 cut(s) | 255, 459, 740 |
| BseYI | CCCAGC | 1 cut(s) | 351 |
| BshFI | GGCC | 1 cut(s) | 626 |
| BshNI | GGYRCC | 2 cut(s) | 424, 709 |
| BshVI | ATCGAT | 1 cut(s) | 93 |
| BsiHKAI | GWGCWC | 3 cut(s) | 17, 190, 250 |
| BsiHKCI | CYCGRG | 1 cut(s) | 700 |
| BslFI | GGGAC | 2 cut(s) | 682, 717 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 301, 358, 742 |
| BsmAI | GTCTC | 1 cut(s) | 117 |
| BsmFI | GGGAC | 2 cut(s) | 682, 717 |
| BsmI | GAATGC | 1 cut(s) | 509 |
| BsnI | GGCC | 1 cut(s) | 626 |
| BsoBI | CYCGRG | 1 cut(s) | 700 |
| Bsp1286I | GDGCHC | 4 cut(s) | 17, 190, 250, 427 |
| Bsp1407I | TGTACA | 1 cut(s) | 334 |
| Bsp143I | GATC | 2 cut(s) | 46, 635 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 16 |
| BspACI | CCGC | 2 cut(s) | 284, 1007 |
| BspANI | GGCC | 1 cut(s) | 626 |
| BspCNI | CTCAG | 3 cut(s) | 29, 152, 733 |
| BspDI | ATCGAT | 1 cut(s) | 93 |
| BspLI | GGNNCC | 2 cut(s) | 426, 711 |
| BspMAI | CTGCAG | 1 cut(s) | 757 |
| BspMI | ACCTGC | 1 cut(s) | 218 |
| BspPI | GGATC | 1 cut(s) | 41 |
| BspT107I | GGYRCC | 2 cut(s) | 424, 709 |
| BsrDI | GCAATG | 2 cut(s) | 11, 798 |
| BsrGI | TGTACA | 1 cut(s) | 334 |
| BsrI | ACTGG | 2 cut(s) | 137, 267 |
| BssECI | CCNNGG | 2 cut(s) | 295, 428 |
| BssMI | GATC | 2 cut(s) | 46, 635 |
| BssNAI | GTATAC | 1 cut(s) | 466 |
| BssSI | CACGAG | 1 cut(s) | 207 |
| Bst1107I | GTATAC | 1 cut(s) | 466 |
| Bst2BI | CACGAG | 1 cut(s) | 207 |
| Bst4CI | ACNGT | 3 cut(s) | 296, 499, 582 |
| Bst6I | CTCTTC | 1 cut(s) | 208 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 596, 752, 761 |
| BstAUI | TGTACA | 1 cut(s) | 334 |
| BstC8I | GCNNGC | 1 cut(s) | 246 |
| BstDEI | CTNAG | 6 cut(s) | 16, 119, 139, 649, 741, 988 |
| BstDSI | CCRYGG | 1 cut(s) | 295 |
| BstF5I | GGATG | 2 cut(s) | 373, 438 |
| BstKTI | GATC | 2 cut(s) | 49, 638 |
| BstMAI | GTCTC | 1 cut(s) | 117 |
| BstMBI | GATC | 2 cut(s) | 46, 635 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 361, 478, 596, 650, 752, 761 |
| BstSFI | CTRYAG | 2 cut(s) | 578, 753 |
| BstSLI | GKGCMC | 2 cut(s) | 250, 427 |
| BstV1I | GCAGC | 3 cut(s) | 255, 459, 740 |
| BstZ17I | GTATAC | 1 cut(s) | 466 |
| Bsu15I | ATCGAT | 1 cut(s) | 93 |
| BsuRI | GGCC | 1 cut(s) | 626 |
| BsuTUI | ATCGAT | 1 cut(s) | 93 |
| BtgI | CCRYGG | 1 cut(s) | 295 |
| BtsCI | GGATG | 2 cut(s) | 373, 438 |
| BtsI | GCAGTG | 1 cut(s) | 692 |
| BtsIMutI | CAGTG | 5 cut(s) | 147, 504, 587, 692, 936 |
| BveI | ACCTGC | 1 cut(s) | 218 |
| Cac8I | GCNNGC | 1 cut(s) | 246 |
| CaiI | CAGNNNCTG | 1 cut(s) | 364 |
| Cfr13I | GGNCC | 2 cut(s) | 676, 851 |
| ClaI | ATCGAT | 1 cut(s) | 93 |
| CseI | GACGC | 1 cut(s) | 804 |
| Csp6I | GTAC | 3 cut(s) | 335, 617, 985 |
| CviAII | CATG | 4 cut(s) | 21, 115, 191, 622 |
| CviQI | GTAC | 3 cut(s) | 335, 617, 985 |
| DdeI | CTNAG | 6 cut(s) | 16, 119, 139, 649, 741, 988 |
| DpnI | GATC | 2 cut(s) | 48, 637 |
| DpnII | GATC | 2 cut(s) | 46, 635 |
| Eam1104I | CTCTTC | 1 cut(s) | 208 |
| EarI | CTCTTC | 1 cut(s) | 208 |
| Ecl136II | GAGCTC | 1 cut(s) | 188 |
| Eco24I | GRGCYC | 1 cut(s) | 190 |
| Eco47I | GGWCC | 2 cut(s) | 676, 851 |
| Eco53kI | GAGCTC | 1 cut(s) | 188 |
| Eco57I | CTGAAG | 6 cut(s) | 206, 341, 385, 497, 585, 930 |
| Eco88I | CYCGRG | 1 cut(s) | 700 |
| EcoICRI | GAGCTC | 1 cut(s) | 188 |
| EcoO109I | RGGNCCY | 1 cut(s) | 851 |
| EcoRI | GAATTC | 2 cut(s) | 150, 1000 |
| EcoT22I | ATGCAT | 1 cut(s) | 874 |
| EcoT38I | GRGCYC | 1 cut(s) | 190 |
| FaeI | CATG | 4 cut(s) | 24, 118, 194, 625 |
| FaqI | GGGAC | 2 cut(s) | 682, 717 |
| FatI | CATG | 4 cut(s) | 20, 114, 190, 621 |
| FauI | CCCGC | 1 cut(s) | 1000 |
| FblI | GTMKAC | 1 cut(s) | 465 |
| Fnu4HI | GCNGC | 4 cut(s) | 269, 285, 473, 729 |
| FokI | GGATG | 2 cut(s) | 380, 445 |
| FriOI | GRGCYC | 1 cut(s) | 190 |
| Fsp4HI | GCNGC | 4 cut(s) | 269, 285, 473, 729 |
| FspBI | CTAG | 3 cut(s) | 864, 917, 960 |
| GluI | GCNGC | 4 cut(s) | 269, 285, 473, 729 |
| GsaI | CCCAGC | 1 cut(s) | 355 |
| GsuI | CTGGAG | 1 cut(s) | 284 |
| HaeIII | GGCC | 1 cut(s) | 626 |
| HgaI | GACGC | 1 cut(s) | 804 |
| Hin1II | CATG | 4 cut(s) | 24, 118, 194, 625 |
| HincII | GTYRAC | 1 cut(s) | 327 |
| HindII | GTYRAC | 1 cut(s) | 327 |
| HindIII | AAGCTT | 1 cut(s) | 609 |
| HinfI | GANTC | 5 cut(s) | 200, 216, 371, 560, 884 |
| HphI | GGTGA | 3 cut(s) | 284, 340, 980 |
| Hpy166II | GTNNAC | 6 cut(s) | 248, 327, 348, 466, 575, 692 |
| Hpy188I | TCNGA | 5 cut(s) | 404, 742, 784, 877, 889 |
| Hpy188III | TCNNGA | 5 cut(s) | 179, 239, 443, 557, 702 |
| Hpy8I | GTNNAC | 6 cut(s) | 248, 327, 348, 466, 575, 692 |
| HpyAV | CCTTC | 7 cut(s) | 87, 167, 230, 269, 313, 499, 864 |
| HpyCH4III | ACNGT | 3 cut(s) | 296, 499, 582 |
| HpyCH4IV | ACGT | 1 cut(s) | 860 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 88, 248, 658, 728, 755, 791, 803, 872 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 361, 478, 596, 650, 752, 761 |
| HpyF3I | CTNAG | 6 cut(s) | 16, 119, 139, 649, 741, 988 |
| HpySE526I | ACGT | 1 cut(s) | 860 |
| Hsp92II | CATG | 4 cut(s) | 24, 118, 194, 625 |
| Kzo9I | GATC | 2 cut(s) | 46, 635 |
| LmnI | GCTCC | 1 cut(s) | 265 |
| Lsp1109I | GCAGC | 3 cut(s) | 255, 459, 740 |
| LweI | GCATC | 4 cut(s) | 586, 715, 881, 919 |
| MaeI | CTAG | 3 cut(s) | 864, 917, 960 |
| MaeII | ACGT | 1 cut(s) | 860 |
| MaeIII | GTNAC | 1 cut(s) | 955 |
| MalI | GATC | 2 cut(s) | 48, 637 |
| MboI | GATC | 2 cut(s) | 46, 635 |
| MboII | GAAGA | 3 cut(s) | 195, 531, 923 |
| MfeI | CAATTG | 1 cut(s) | 792 |
| MhlI | GDGCHC | 4 cut(s) | 17, 190, 250, 427 |
| MluCI | AATT | 6 cut(s) | 150, 535, 747, 792, 805, 1000 |
| MlyI | GAGTC | 2 cut(s) | 194, 210 |
| MmeI | TCCRAC | 1 cut(s) | 826 |
| MnlI | CCTC | 9 cut(s) | 18, 21, 24, 423, 481, 558, 659, 736, 842 |
| Mph1103I | ATGCAT | 1 cut(s) | 874 |
| MroXI | GAANNNNTTC | 1 cut(s) | 509 |
| MseI | TTAA | 1 cut(s) | 60 |
| MspA1I | CMGCKG | 1 cut(s) | 1007 |
| MunI | CAATTG | 1 cut(s) | 792 |
| Mva1269I | GAATGC | 1 cut(s) | 509 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 361, 478, 596, 650, 752, 761 |
| NdeII | GATC | 2 cut(s) | 46, 635 |
| NlaIII | CATG | 4 cut(s) | 24, 118, 194, 625 |
| NlaIV | GGNNCC | 2 cut(s) | 426, 711 |
| NsiI | ATGCAT | 1 cut(s) | 874 |
| PctI | GAATGC | 1 cut(s) | 509 |
| PdmI | GAANNNNTTC | 1 cut(s) | 509 |
| PfeI | GAWTC | 3 cut(s) | 371, 560, 884 |
| PkrI | GCNGC | 4 cut(s) | 270, 286, 474, 730 |
| PleI | GAGTC | 2 cut(s) | 194, 210 |
| PpsI | GAGTC | 2 cut(s) | 194, 210 |
| PpuMI | RGGWCCY | 1 cut(s) | 851 |
| Psp124BI | GAGCTC | 1 cut(s) | 190 |
| Psp5II | RGGWCCY | 1 cut(s) | 851 |
| PspFI | CCCAGC | 1 cut(s) | 351 |
| PspN4I | GGNNCC | 2 cut(s) | 426, 711 |
| PspPI | GGNCC | 2 cut(s) | 676, 851 |
| PspPPI | RGGWCCY | 1 cut(s) | 851 |
| PstI | CTGCAG | 1 cut(s) | 757 |
| PstNI | CAGNNNCTG | 1 cut(s) | 364 |
| RsaI | GTAC | 3 cut(s) | 336, 618, 986 |
| RsaNI | GTAC | 3 cut(s) | 335, 617, 985 |
| SacI | GAGCTC | 1 cut(s) | 190 |
| SaqAI | TTAA | 1 cut(s) | 60 |
| SatI | GCNGC | 4 cut(s) | 269, 285, 473, 729 |
| Sau3AI | GATC | 2 cut(s) | 46, 635 |
| Sau96I | GGNCC | 2 cut(s) | 676, 851 |
| ScaI | AGTACT | 1 cut(s) | 986 |
| SchI | GAGTC | 2 cut(s) | 194, 210 |
| SduI | GDGCHC | 4 cut(s) | 17, 190, 250, 427 |
| SfaNI | GCATC | 4 cut(s) | 586, 715, 881, 919 |
| SfcI | CTRYAG | 2 cut(s) | 578, 753 |
| SinI | GGWCC | 2 cut(s) | 676, 851 |
| SmlI | CTYRAG | 1 cut(s) | 158 |
| SmoI | CTYRAG | 1 cut(s) | 158 |
| Sse9I | AATT | 6 cut(s) | 150, 535, 747, 792, 805, 1000 |
| SsiI | CCGC | 2 cut(s) | 284, 1007 |
| SspMI | CTAG | 3 cut(s) | 864, 917, 960 |
| SstI | GAGCTC | 1 cut(s) | 190 |
| TaaI | ACNGT | 3 cut(s) | 296, 499, 582 |
| TaiI | ACGT | 1 cut(s) | 863 |
| TaqI | TCGA | 4 cut(s) | 69, 93, 513, 563 |
| TasI | AATT | 6 cut(s) | 150, 535, 747, 792, 805, 1000 |
| TatI | WGTACW | 2 cut(s) | 334, 984 |
| TauI | GCSGC | 1 cut(s) | 287 |
| TfiI | GAWTC | 3 cut(s) | 371, 560, 884 |
| Tru1I | TTAA | 1 cut(s) | 60 |
| Tru9I | TTAA | 1 cut(s) | 60 |
| TscAI | CASTG | 5 cut(s) | 147, 504, 587, 699, 943 |
| TseI | GCWGC | 3 cut(s) | 268, 472, 728 |
| TspDTI | ATGAA | 3 cut(s) | 103, 332, 384 |
| TspRI | CASTG | 5 cut(s) | 147, 504, 587, 699, 943 |
| VneI | GTGCAC | 1 cut(s) | 246 |
| VpaK11BI | GGWCC | 2 cut(s) | 676, 851 |
| XapI | RAATTY | 3 cut(s) | 150, 805, 1000 |
| XmiI | GTMKAC | 1 cut(s) | 465 |
| XmnI | GAANNNNTTC | 1 cut(s) | 509 |
| XspI | CTAG | 3 cut(s) | 864, 917, 960 |
| ZrmI | AGTACT | 1 cut(s) | 986 |
| Zsp2I | ATGCAT | 1 cut(s) | 874 |