NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 199 |
| AcsI | RAATTY | 1 cut(s) | 295 |
| AcuI | CTGAAG | 2 cut(s) | 78, 420 |
| AfaI | GTAC | 2 cut(s) | 111, 476 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 232 |
| AgsI | TTSAA | 4 cut(s) | 53, 101, 348, 443 |
| AluBI | AGCT | 2 cut(s) | 104, 143 |
| AluI | AGCT | 2 cut(s) | 104, 143 |
| Ama87I | CYCGRG | 1 cut(s) | 190 |
| AoxI | GGCC | 1 cut(s) | 117 |
| ApeKI | GCWGC | 1 cut(s) | 218 |
| ApoI | RAATTY | 1 cut(s) | 295 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 120, 152 |
| AspS9I | GGNCC | 1 cut(s) | 166 |
| AsuHPI | GGTGA | 1 cut(s) | 470 |
| AvaI | CYCGRG | 1 cut(s) | 190 |
| AvaII | GGWCC | 1 cut(s) | 166 |
| BanI | GGYRCC | 1 cut(s) | 199 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 93, 125 |
| BbvI | GCAGC | 1 cut(s) | 205 |
| BfaI | CTAG | 2 cut(s) | 354, 407 |
| BfmI | CTRYAG | 2 cut(s) | 71, 243 |
| BisI | GCNGC | 1 cut(s) | 219 |
| BlsI | GCNGC | 1 cut(s) | 220 |
| BmcAI | AGTACT | 1 cut(s) | 476 |
| Bme18I | GGWCC | 1 cut(s) | 166 |
| BmeT110I | CYCGRG | 1 cut(s) | 190 |
| BmgT120I | GGNCC | 1 cut(s) | 166 |
| BmiI | GGNNCC | 1 cut(s) | 201 |
| BmsI | GCATC | 3 cut(s) | 79, 371, 409 |
| BsaJI | CCNNGG | 1 cut(s) | 210 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 232 |
| Bse3DI | GCAATG | 1 cut(s) | 288 |
| BseDI | CCNNGG | 1 cut(s) | 210 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 232 |
| BseMI | GCAATG | 1 cut(s) | 288 |
| BseMII | CTCAG | 1 cut(s) | 222 |
| BseXI | GCAGC | 1 cut(s) | 205 |
| BshFI | GGCC | 1 cut(s) | 119 |
| BshNI | GGYRCC | 1 cut(s) | 199 |
| BsiHKCI | CYCGRG | 1 cut(s) | 190 |
| BslFI | GGGAC | 2 cut(s) | 172, 207 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 232 |
| BsmFI | GGGAC | 2 cut(s) | 172, 207 |
| BsnI | GGCC | 1 cut(s) | 119 |
| BsoBI | CYCGRG | 1 cut(s) | 190 |
| BspANI | GGCC | 1 cut(s) | 119 |
| BspCNI | CTCAG | 1 cut(s) | 223 |
| BspLI | GGNNCC | 1 cut(s) | 201 |
| BspMAI | CTGCAG | 1 cut(s) | 247 |
| BspT107I | GGYRCC | 1 cut(s) | 199 |
| BsrDI | GCAATG | 1 cut(s) | 288 |
| BssECI | CCNNGG | 1 cut(s) | 210 |
| Bst4CI | ACNGT | 2 cut(s) | 7, 75 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 89, 242, 251 |
| BstDEI | CTNAG | 3 cut(s) | 139, 231, 483 |
| BstDSI | CCRYGG | 1 cut(s) | 210 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 89, 242, 251 |
| BstSFI | CTRYAG | 2 cut(s) | 71, 243 |
| BstV1I | GCAGC | 1 cut(s) | 205 |
| BsuRI | GGCC | 1 cut(s) | 119 |
| BtgI | CCRYGG | 1 cut(s) | 210 |
| BtsIMutI | CAGTG | 4 cut(s) | 12, 80, 315, 426 |
| Cfr13I | GGNCC | 1 cut(s) | 166 |
| CseI | GACGC | 2 cut(s) | 224, 294 |
| Csp6I | GTAC | 2 cut(s) | 110, 475 |
| CviAII | CATG | 1 cut(s) | 115 |
| CviJI | RGCY | 5 cut(s) | 104, 119, 143, 390, 482 |
| CviKI_1 | RGCY | 5 cut(s) | 104, 119, 143, 390, 482 |
| CviQI | GTAC | 2 cut(s) | 110, 475 |
| DdeI | CTNAG | 3 cut(s) | 139, 231, 483 |
| Eco47I | GGWCC | 1 cut(s) | 166 |
| Eco57I | CTGAAG | 2 cut(s) | 78, 420 |
| Eco88I | CYCGRG | 1 cut(s) | 190 |
| EcoT22I | ATGCAT | 1 cut(s) | 364 |
| FaeI | CATG | 1 cut(s) | 118 |
| FaiI | YATR | 4 cut(s) | 41, 116, 435, 467 |
| FaqI | GGGAC | 2 cut(s) | 172, 207 |
| FatI | CATG | 1 cut(s) | 114 |
| Fnu4HI | GCNGC | 1 cut(s) | 219 |
| Fsp4HI | GCNGC | 1 cut(s) | 219 |
| FspBI | CTAG | 2 cut(s) | 354, 407 |
| GluI | GCNGC | 1 cut(s) | 219 |
| HaeIII | GGCC | 1 cut(s) | 119 |
| HgaI | GACGC | 2 cut(s) | 224, 294 |
| Hin1II | CATG | 1 cut(s) | 118 |
| HincII | GTYRAC | 1 cut(s) | 182 |
| HindII | GTYRAC | 1 cut(s) | 182 |
| HindIII | AAGCTT | 1 cut(s) | 102 |
| HinfI | GANTC | 2 cut(s) | 53, 374 |
| HphI | GGTGA | 1 cut(s) | 470 |
| Hpy166II | GTNNAC | 2 cut(s) | 68, 182 |
| Hpy188I | TCNGA | 4 cut(s) | 232, 274, 367, 379 |
| Hpy188III | TCNNGA | 1 cut(s) | 192 |
| Hpy8I | GTNNAC | 2 cut(s) | 68, 182 |
| HpyAV | CCTTC | 1 cut(s) | 7 |
| HpyCH4III | ACNGT | 2 cut(s) | 7, 75 |
| HpyCH4V | TGCA | 5 cut(s) | 148, 245, 281, 293, 362 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 89, 242, 251 |
| HpyF3I | CTNAG | 3 cut(s) | 139, 231, 483 |
| Hsp92II | CATG | 1 cut(s) | 118 |
| LpnPI | CCDG | 3 cut(s) | 134, 182, 255 |
| Lsp1109I | GCAGC | 1 cut(s) | 205 |
| LweI | GCATC | 3 cut(s) | 79, 371, 409 |
| MaeI | CTAG | 2 cut(s) | 354, 407 |
| MboII | GAAGA | 2 cut(s) | 27, 413 |
| MfeI | CAATTG | 1 cut(s) | 282 |
| MluCI | AATT | 4 cut(s) | 31, 237, 282, 295 |
| MmeI | TCCRAC | 1 cut(s) | 316 |
| MnlI | CCTC | 3 cut(s) | 149, 226, 332 |
| Mph1103I | ATGCAT | 1 cut(s) | 364 |
| MseI | TTAA | 1 cut(s) | 447 |
| MunI | CAATTG | 1 cut(s) | 282 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 89, 242, 251 |
| NlaIII | CATG | 1 cut(s) | 118 |
| NlaIV | GGNNCC | 1 cut(s) | 201 |
| NsiI | ATGCAT | 1 cut(s) | 364 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 218 |
| PfeI | GAWTC | 2 cut(s) | 53, 374 |
| PkrI | GCNGC | 1 cut(s) | 220 |
| PspN4I | GGNNCC | 1 cut(s) | 201 |
| PspPI | GGNCC | 1 cut(s) | 166 |
| PstI | CTGCAG | 1 cut(s) | 247 |
| RsaI | GTAC | 2 cut(s) | 111, 476 |
| RsaNI | GTAC | 2 cut(s) | 110, 475 |
| SaqAI | TTAA | 1 cut(s) | 447 |
| SatI | GCNGC | 1 cut(s) | 219 |
| Sau96I | GGNCC | 1 cut(s) | 166 |
| ScaI | AGTACT | 1 cut(s) | 476 |
| SetI | ASST | 5 cut(s) | 51, 106, 145, 343, 460 |
| SfaNI | GCATC | 3 cut(s) | 79, 371, 409 |
| SfcI | CTRYAG | 2 cut(s) | 71, 243 |
| SinI | GGWCC | 1 cut(s) | 166 |
| Sse9I | AATT | 4 cut(s) | 31, 237, 282, 295 |
| SspMI | CTAG | 2 cut(s) | 354, 407 |
| TaaI | ACNGT | 2 cut(s) | 7, 75 |
| TasI | AATT | 4 cut(s) | 31, 237, 282, 295 |
| TatI | WGTACW | 1 cut(s) | 474 |
| TfiI | GAWTC | 2 cut(s) | 53, 374 |
| Tru1I | TTAA | 1 cut(s) | 447 |
| Tru9I | TTAA | 1 cut(s) | 447 |
| TscAI | CASTG | 4 cut(s) | 12, 80, 315, 433 |
| TseI | GCWGC | 1 cut(s) | 218 |
| TspGWI | ACGGA | 1 cut(s) | 227 |
| TspRI | CASTG | 4 cut(s) | 12, 80, 315, 433 |
| VpaK11BI | GGWCC | 1 cut(s) | 166 |
| XapI | RAATTY | 1 cut(s) | 295 |
| XspI | CTAG | 2 cut(s) | 354, 407 |
| ZrmI | AGTACT | 1 cut(s) | 476 |
| Zsp2I | ATGCAT | 1 cut(s) | 364 |